KR20020032506A - Hematic green tea and method for preparation thereof - Google Patents

Abstract

PURPOSE: Provided is a process of preparing green tea(Camellia sinensis) by adding Chinese herbal medicine having erythropoietic and blood circulating activity to green tea as a main material. Whereby, the product has increased erythropoietic activity by a synergistic effect and no side effects and toxicity. CONSTITUTION: A mixture of 25 to 50 parts by weight of Angelicae gigantis radix, 25 to 50 parts by weight of Cnidii Rhizoma and 25 to 50 parts by weight of Paeonia lactiflora is heated and dried, followed by grinding to give a herbal erythropoietic agent, which is mixed with Camellia sinensis powder in a ratio of 6:4 to 8:2, added with roasted unpolished powder in a ratio of 2:1 to 4:1 and extracted at 100deg.C for 30 min to 1 hr. The extract is mixed with a Camellia sinensis extract extracted at 75 to 85deg.C for 20 to 30 min and then added with 0.02 to 0.04%(w/v%) and liquid spice.

Description

Hematopoietic green tea and method for preparing the same

The present invention relates to a hematopoietic green tea and a beverage production method for cans thereof. More specifically, the preparation of functional green tea herbal hematopoietic agent and its green tea herbal based on the results of hematopoietic efficacy test through animal experiments after preparing and blending green tea and herbal medicine in a suitable blending ratio with hematopoietic tea The present invention relates to a preparation method of hematopoietic green tea for liquid can beverage having functional and palatability by blending powdered brown rice which is a flavor enhancer based on a hematopoietic agent and adding a liquid flavoring agent.

The tea tree is a perennial evergreen shrub ( Camellia Sinensis ) belonging to the Camellia family. The first tea drink originated as a medicinal drink and then changed into a symbolic drink. Today, more than 50% of the world's drink is consumed. Tea, which has a long history and various pharmacological effects as a non-alcoholic beverage, has been found to be a functional food because many research results on the preference foods that enjoy flavor and taste according to the general classification of food and recent tea have been revealed.

Among the components of tea, there are three kinds of chemicals that are related to the efficacy of the human body: catechin, caffeine, and vitamins. Especially, catechin, which is a polyphenol component having a tertiary function, is 10-18% in tea leaves. It contains a lot of functions. In other words, catechins, which show various physiological activities of tea, have vascular strengthening, platelet aggregation suppression, and antithrombotic function in relation to blood circulation, prolong bleeding, coagulation time, and blood pressure strengthening (血壓 强化 作用) It is known to facilitate blood circulation. The reason for the above pharmacological action is that catechins have a large number of hydroxyl groups (OH) due to their chemical structure, and easily combine with other substances to change and inhibit the properties of the substances. In addition, the excellent detoxification effect of catechins is effective to neutralize the harm from overdose, accumulation and toxicity of medicines. And antioxidants.

Nerve stimulating caffeine activity has pharmacological effects such as excitement, cardiac, diuresis, antipyretic, convergence, etc. It is inhibited by catechin and selectively controlled by theanine component to stimulate central nervous system By acting, fast-acting (速效性) pharmacological action appears. Physiological effects such as fast action of caffeine have synergistic antagonism with medicinal and useful ingredients to increase the active effect. Especially, the results of animal experiments are known that the antagonism of caffeine in the hematopoietic herbal medicines for sedation ('90, Korean medicine, '90, Korean medicine).

Tea is also known to contain tea leaf folic acid, rich iron and vitamin B ₁₂ , which help erythrocyte growth and treat pernicious anemia. Vitamin C is known to increase resistance by preventing capillary rupture.

On the other hand, Samul-tang, which forms the basis of hemostasis and hematopoietic drugs, is a typical prescription medicine for hemophilia (인데), which is used for blood production, generalization, and storage. All blood-related diseases (血液 關聯 性 疾患), blood clots are prescribed to cure (agreement).

The basic ingredients of Samultang are Sukji Hwang, Angelica, Baekjak and Cheongung. Sukji Hwang (Mature 地 黄) to see the blood, donggui (當歸) blood and good blood flow, Baekjak (白 灼 藥) stops the blood and pain, Cheonggung (川 芎) to turn the blood well. In the basic medical research, Samul-tang has high efficacy in recovering anemia. Clinical studies have shown a clear therapeutic effect on dysmenorrhea and painful dysmenorrhea (Agreement, '90, Korean Remedy).

According to Chabo 茶 補 and Chakkyung 茶 經, "tea is used for the treatment of headaches and brain pains." Traditionally, tea leaves have been used to treat headaches. Thus, the treatment method using a combination of different herbal medicines in green tea is called "commercial tea room" and has been an important part of Chinese medicine since ancient times (옛 用 茶 療 方). It encompasses the characteristics of tea and the efficacy of tea leaves, but also exerts the effects that tea leaves do not possess.It helps to show the therapeutic effect of drugs by combining with other herbs, promotes the dissolution of drugs, and increases the fragrance to enhance the taste. The prescription is characterized by the fact that it is refined and convenient to drink, and the range of tea room using tea and herbal medicine is extensive.

However, functional foods made by adding herbal medicines to green tea are characterized by strong Chinese herbal smells that vary in preference according to human preference. In the case of tea using such herbal medicine, it was obvious that the smell was not good and there was a lot of difficulty in developing a favorite food using herbal medicine. In the meantime, numerous studies on the functionality of tea have been published, and the product development is diverse. However, the development of products incorporating green tea and herbal medicines is almost limited due to the efficacy and palatability of the developed tea, and the development of products using the same is rare.

The present inventors expect that the effect of the green tea as the main ingredient and mixed with the medicinal herbs that are effective for hematopoiesis and blood circulation will be further increased by their pharmacological activity and synergism, rather than the green tea or herbal hematopoietic agents alone. And as a result of animal experiments on hematopoiesis, it was confirmed that the effect on hematopoiesis and hematopoiesis was excellent, and based on these results, it was confirmed that a tea having excellent functionality and palatability was produced to complete the present invention.

In addition, these hematopoietic medicines are known to have more effective effects in complex prescriptions than in single prescriptions in clinical and pharmacological experiments, and the components of their efficacy are known by recent advances in instrumental analysis (91, 科 の 科學, 新茶 業 全書, '84, Ingredients and Uses of Herbs). The present invention is different from the conventional invention of simple extraction and liquid or granules by combining single medicines or medicines having the same efficacy as general inventions, and teas in which individual medicines are mixed and applied for inconsistency only for health purposes. Subsequently, existing herbal medicines, which are excluded from palatability and functionality, or by simple extraction and granulation of herbal medicines, have a strong therapeutic purpose and cannot be used as ordinary teas.

The present invention is related to the hematopoietic efficacy and expanded the application of commercial tea room with tea as a main medicine to the modern times the abdominal mass of the well known in the field is different from other inventions in that the maximum use of the efficacy, such as Samultang In addition, among the hematopoietic drugs that are the basis of Samul-tang, the feature of the present invention is that the hematopoietic efficacy is increased as much as possible by removing the sulfur of Sukji to minimize the side effects that may appear in the human body.

In addition, rather than just making a healthy tea, as the general invention, the green tea herbal hematopoietic treatment of the present invention has been proved to be excellent in blood circulation and hematopoietic activity through animal experiments, thereby focusing on the main functionality and palatability of the present invention. It is to contribute to the health of the people by making it possible to drink as a daily drink. Therefore, to improve the palatability by adding a flavor enhancer to remove the strong odor of herbal medicine that tends to appear in tea using herbal medicine, to manufacture a liquid canned hematopoietic green tea as a hematopoietic tea that solved the functionality and palatability of the present invention It proved to be a very good base material.

These teas are useful as treatment aids or preventives for general women, postpartum mothers, blood-deficiency diseases, elderly menopausal, poor general people and postoperative bleeding patients, such as functional, palatability, popularity, universality, etc. Considering the quality of bodhisattva blood of excellent quality.

Therefore, an object of the present invention is to provide a hematopoietic tea excellent in functionality as a green tea herbal hematopoietic agent mixed with green tea and herbal medicine having a hematopoietic effect.

In addition, another object of the present invention is to improve the flavor by relieving the strong aroma and taste peculiar to Chinese medicine to increase the palatability, and can drink easily drink anytime, anywhere as a highly functional palatability tea to increase the storage stability and hematopoietic efficacy of the product It is to provide a method for producing a liquid tea.

In addition, the main object of the present invention is to increase the hematopoietic effect by increasing the pharmacological synergism by the interaction of green tea and herbal medicine while maintaining the efficacy of the green tea and the efficacy of the herbal medicine as described above, on the other hand, Taking into consideration the side effects and toxicity of this tea, it is to provide functional health tea that contributes to national health by supplementing and preventing ischemia according to usual drinking as usual food.

1 is a process chart showing a process for preparing green tea herbal hematopoietic agent and powdered brown rice according to the present invention.

Figure 2 is a process chart showing the process for producing a hematopoietic green tea for cans beverages according to the present invention.

The present invention is not mixed with 25-50 parts by weight, 25-50 parts by weight of Cheongung and 25-50 parts by weight of Baekjeok, prepared and powdered herbal hematopoietic agents in green tea powder and dry weight ratio 6: 4-8: 2. It is made with green tea herbal hematopoietic agent, and brown rice is mixed with green tea herbal hematopoietic agent in a dry weight ratio of 2: 1 to 4: 1, followed by vitamin C 0.02 to 0.04% (w / v%) and liquid flavor 0.02 to 0.06% (v / v%). It is a hematopoietic green tea for liquid canned beverage added.

In addition, the present invention is the first step to obtain a herbal hematopoietic agent by mixing 25 to 50 parts by weight, 25 to 50 parts by weight and 25 to 50 parts by weight of the arch, and steaming, drying and powdered herbal medicine obtained in the first step The hematopoietic agent was quantified with green tea powder and dry weight ratio 6: 4 to 8: 2 to obtain unmixed green tea herbal blood preparation, steamed brown rice, and then roasted brown rice powder at 100 to 180 ° C. with dry weight ratio 2: In the second and second steps to quantify to 1 to 4: 1, the first extraction filtration of herbal hematopoietic agent and brown rice powder of green tea herbal hematopoietic agent, and the first extract and secondary extract obtained by the second extraction filtration of green tea After filtration under reduced pressure, vitamin C 0.02 to 0.04% (w / v%) and liquid chemical 0.02 to 0.06% (v / v%) are added. It is a manufacturing method of sex green tea.

Hereinafter, the present invention will be described in detail.

Herbal hematopoietic agent, the main component of the hematopoietic green tea of the present invention, is to remove only Suk-ji-sul, which is the base material of Samultang, Angelica, Cheon-gung, Suk-ji-hwang, and Baekjak. This is not used when Sukjihwang is not palatable and difficult to digest, and the medicine is put in a copper or iron bowl, and if it is legally or decocted, the condition is damaged and the hair is whitened, radish, garlic, green onions are excluded.

These Angelica sinensis, Cheongung and Baekjak respectively can be combined in 25 to 50 parts by weight can maximize their pharmacological activity. Preferably Angelica, Cheongung and Baekjak are formulated in a dry weight ratio of about 1: 1: 1.

In addition, as the green tea which is another main component of the hematopoietic green tea of the present invention, it is preferable to use general green tea powder, and to use the tea leaves collected in May.

In addition, it is preferable that the herbal hematopoietic agent and the green tea are blended in a dry weight ratio of 6: 4 to 8: 2 to increase the hematopoietic efficacy by pharmacological synergism between them.

That is, the present invention is a hematopoietic green tea excellent in functionality prepared by using green tea as a main medicine and a natural herbal medicine that is efficacious in hematopoiesis using a method of using high-availability commercial drugs as described above.

In addition, the hematopoietic green tea of the present invention is blended with brown rice powder in a dry weight ratio of 2: 1 to 4: 1 and powdered flavoring 0.02 to 0.06% (w / w%) is added.

Hematopoietic green tea, developed on the basis of the above-mentioned effects, has a lot of disorders of blood circulation due to various environmental pollution, pollution, stress, etc. for modern people, and especially prevents anemia of women and growing children, ) Is well applied to modern people because it applies the pathological characteristics of ischemic patients and the pharmacological characteristics of commercial medicines.

Hereinafter, a method of preparing canned hematopoietic green tea and an animal test for functional verification of blood circulation and hematopoietic efficacy will be described in detail.

The manufacturing method of hematopoietic green tea is demonstrated as follows for each process.

Step 1: Preparation of Herbal Hematopoietic Agents

The basic materials of herbal hematopoietic agent, Angelica, Cheongung, and Baekjak are quantitatively weighed 25 to 50 parts by weight, and then carefully selected and lightly washed. Each medicine is steamed until steam comes out of the steamer. The steamed medicines are spread on the foot for drying tea and dried to 80% or less at a drying temperature of 5% or less.

The herbal hematopoietic agents are pulverized with a mesh size of about 4.0 to 2.0 mm in a mill to obtain the herbal hematopoietic agents as powders.

Second Step: Preparation of Green Tea Herbal Hematopoietic Agent

end. Manufacture of Green Tea

Cultivated tea leaves are harvested and the tea is made from green tea raw materials according to the standard process of the first tea for steamed tea. The green tea is pulverized in a grinder with a mesh size of about 4.0 to 2.0 mm to obtain green tea as a powder.

I. Herbal Hematopoietic Agent and Green Tea Mixing Process

The herbal hematopoietic powder obtained in the first step and the green tea powder are quantified in dry weight ratio (w / w%) 6: 4 to 8: 2, respectively, to obtain a non-mixed green tea herbal hematopoietic agent for canned beverage.

All. Brown Rice Pretreatment and Powder Manufacturing Process

Green tea herbal hematopoietic obtained in the present invention tends to be strong herbal smell and bitter taste, astringent taste when hot water extraction when used as a liquid canned beverage product is an important problem to improve their taste and to improve the overall flavor harmony . Therefore, the pretreatment process of brown rice for blending the powdered roasted brown rice in the hematopoietic green tea to improve the flavor so that anyone can enjoy drinking without refusing when drinking.

After steaming and washing brown rice, steam the steamed rice at 100 ℃ for 30 minutes to 1 hour, and then make spicy rice. Spread it on the dryer's foot and hot-air-dried at 80 ℃ for drying for 3 to 4 hours. . In the above steaming process is usually a steaming process at home.

Set the temperature of the cauldron for simmering to 100-180 ° C. and fry until it reaches slightly yellow color so as not to burn the pretreated brown rice. Roasted brown rice is grind | pulverized by the mesh size of 4.0-2.0 mm in a grinder, and brown rice powder is obtained.

The green tea herbal hematopoietic agent and the pretreated powdered brown rice are quantified in a dry weight ratio of 2: 1 to 4: 1 to obtain a hematopoietic green tea having enhanced flavor.

In the conventional processing method of brown rice mixed with green tea, there are many examples of using brown rice immediately after washing and washing with brown rice, or drying and washing it after washing with water. In this method, the appearance density of brown rice is higher than that of green tea when processing brown rice and green tea, and when brown rice and green tea are mixed during processing, only brown rice tends to be driven to one side, so it is not mixed well. In addition, the leaching of brown rice and green tea prepared in this manner in hot water has a disadvantage in that the starch is less gelatinous, so that the loosening and leaching is poor and the deterioration of the flavor is greatly reduced.

However, since the processing method of brown rice used in the present invention is steamed and then roasted and roasted, the luxury of starch is increased, while the brown rice is slightly swollen and the surface color becomes pale yellow during roasting. have. In addition, the increase in gelatinization of starch has the advantage of being well mixed with other powdery mixtures after grinding by reducing the appearance density, and is characterized by high loosening and leaching properties for hot water and strong flavor when leaching. Therefore, the brown rice powder thus prepared is preferably used as an auxiliary ingredient which is a flavor enhancer.

3rd step: process for commercializing hematopoietic green tea for liquid can drink

Herbal hematopoietic powder and green tea powder are prepared by quantifying 6: 4 to 8: 2, and brown rice powder is prepared by quantifying the herbal hematopoietic agent and green tea at a ratio of 2: 1 to 4: 1. When the total composition ratio (w / w%) of each powder is 200 g before canned beverage extraction, the amount of hot water to be extracted is based on 17 to 22 liters.

As a primary extraction method, among the powdered green tea and herbal hematopoietic agent, first mix the herbal hematopoietic agent and brown rice into the extractor, add 12-15 liters of hot water with ion exchange resin, and stir at 100 ° C for 30 minutes to 1 hour (adjusted according to the extraction amount). Extraction and centrifugation in a conventional manner, followed by filtration. When the hot water temperature is 75 to 85 ° C. in the same manner as the primary extraction, the green tea is added by stirring 5 ~ 7 L of hot water and stirred for 20 to 30 minutes (adjusted according to the extraction amount), followed by centrifugation and filtration. The primary and secondary extracts are cooled to about 20 ° C. using a coolant, filtered through a vacuum filter, and mixed. Vitamin C 0.02 to 0.04% (w / v%) and liquid fragrance 0.02 to 0.06% (v / v%) are added to the mixed extract, and heated to 85 to 95 ° C in a planar heat exchanger. Charge when hot. After filling the mixed extract, nitrogen gas is injected into the headspace of the canned beverage to reduce the dissolved oxygen of the product and then sealed with a can lid. Sealed can products are sterilized and cooled to be commercialized.

Here, the first extraction of brown rice and herbal hematopoietic agent, and the second extraction of green tea is separately extracted because the flavor loss and chlorophyll and vitamins unique to green tea are destroyed at the temperature of the first extraction.

In addition, the extraction method of herbal tea or native traditional tea using the traditional herbal medicine has a problem that the extraction time takes about 4 to 5 hours, and the extraction is not easy because many examples of extracting the herbal medicine immediately without going through the pretreatment process. However, the extraction method according to the present invention went through the pre-treatment process of powdering the green tea and herbal medicine through the process of steaming, drying first, can be immediately entered into the extraction process for beverage extraction, processed powder state is easy to long-term storage and easy to extract It is a feature of the present invention that the extraction time is greatly shortened. The hematopoietic green tea for canned beverage obtained by carrying out in this manner was able to obtain a uniform quality beverage having good hot water leaching during processing, shortening extraction time and excellent functionality and palatability.

Loss of vitamin C by hot water during canned beverages causes physiological activity and antioxidant activity to decrease the tea's functionality and storage stability. This is because vitamin C has a synergistic effect and a useful ingredient that has the functions of green tea and herbal medicine as antioxidants, and has a physiological activity enhancing and antioxidative effect and helps to absorb Fe ²⁺ . In addition, the polyphenol component of green tea promotes antioxidant and physiological activity in the body when coexisting with caffeine, vitamin C, vitamin E, etc. There are many reports of pharmacological rise caused by caffeine.

Therefore, the supplementation strengthening of vitamin C, which forms an elevation with the polyphenol component of tea, and the filling of nitrogen gas in the head space just before the can lid closure minimizes the amount of dissolved oxygen in the beverage, thereby increasing the storage stability and preventing oxidation of the product. The addition of roasted brown rice and spices alleviated the strong odor of herbal medicines, which solved the palatability problem of herbal teas, and it was possible to obtain smooth and delicious flavored teas.

As described above, the liquid canned hematopoietic green tea prepared according to the present invention can produce hematopoietic supplements having excellent functionality as a health tea, and have excellent shelf life, palatability, functionality, and simplicity, and are popular foods or functionalities of modern people. It is very suitable as a health tea.

Preparation of hematopoietic dietary group and verification of hematopoietic effect through animal experiment

end. Research contents

A decrease in the amount of red blood cells and the concentration of hemoglobin due to a lack of iron as a raw material of hemoglobin, a lack of erythrocyte production due to abnormal and unstable production of erythrocytes, bleeding, and hemolysis results in a hypoxic state of the body tissues. Becomes Hepatic tissue is a major organ that stores iron, especially hemoglobin, which stimulates the liver and bone marrow after bleeding to release hepatic iron, and stimulation of bone marrow increases the synthesis of red blood cells. To increase the action of binding. Fast free storage of liver and stimulation of bone marrow in the liver is a factor in the rapid treatment of ischemic symptoms after bleeding. Among the treatments for anemia, it is important to stimulate metabolism by pharmacotherapy and balanced nutritional intake to promote red blood cell production, hemoglobin synthesis and liver absorption or free activity necessary for liver storage.

In order to investigate whether the green tea herbal preparation of the present invention has a hematopoietic effect, anemia should be induced in animal experiments. Among the various methods of inducing anemia in experimental animals, ischemia is a method of inducing anemia symptoms, such as lowering the concentration of hemoglobin in the blood or removing blood from a part of the body, resulting in hypoxia. Representative methods are hemolysis and bleeding. Among them, the bleeding method is a therapeutic method, which is a good way to promote the production of red blood cells by nutrient therapy and balanced nutrition in general.

In the present study, a group of hematopoietic group containing 1% of green tea herbal hematopoietic extract was added to a normal diet group (Table 1), and oral administration of medicinal herbs for 4 days before bleeding and oral administration for 7 days after bleeding. Hematopoietic effect was searched by. The ischemic induction method was induced by anemia using bleeding method and observed recovery stereotyping with erythrocyte count, hemoglobin concentration, erythrocyte volume percentage, and reticulocyte count. The control group was also orally administered to the normal diet group to search for the hematopoietic effect in the same manner as the hematopoietic diet group.

I. Experimental Materials and Methods

(1) Experimental animal: Sprague-Dawley male rat

Table 1. Composition of Normal Dietary Group (Control, g / kg)

Dietary castle Casein Corn starch Sucrose Corn Oil Cellulose DL-Methionine min mix * (AIN 76) vit mix ** (AIN 76) cholinebitartrate Metabolic Energy Consumption Compounding ratio 200 521 100 100 30 2 35 10 2 4.12 ㎉ / g

*: min mix: Multi Mineral, **: vit mix: Multi Vitamin

◆ hematopoietic dietary group: normal dietary group + 1% green tea herbal extracts

(2) Duration of experiment: 11 days (4 days before bleeding medicine + 7 days after bleeding)

(3) Measurement contents and method

(A) body weight gain,

(B) food intake,

(C) Red blood cell count: Use of coulter counter

Hemoglobin concentration: Hemoglobin concentration in blood reacts with potassium ferricyanide to be converted into methamolobin and reacts with potassium cyanide to convert to cyanmethemoglobin to measure absorbance at 540 nm. Hemoglobin was used to determine the amount of hemoglobin and measured using a commercially available reagent for measurement in Asan Pharmaceutical.

(E) Hematocrit percentage (hematocrit): Measured by withholding precipitation method.

Reticulocyte count: The blood was stained with fresh methylene blue and the reticulocyte count was examined under a microscope. The quantitative indication was expressed as the number of reticulocytes during counting 1,000 RBCs.

Hereinafter, the present invention will be described in detail with reference to Examples.

Example 1

Preparation of Liquid Hematopoietic Green Tea for Canned Drinks

Herbal hematopoietic agent was selected as the tangui, cheongung, Baekjak etc. according to the ratio (Table 2) exemplified below, quantified by dry weight ratio for each equal portion and washed with water. After washing, mix each medicine and put it in a pressure steamer and steam it. At this time, the amount of water was about twice the volume of the medicine volume, poured water and steamed in the pressure steamer. The steamed medicines were spread on the foot for drying the tea and dried to 80% or less at a drying temperature of 5% or less as a herbal hematopoietic agent. Be careful not to burn when steaming with steaming medicine in the pressure steamer.If there is a small amount of excess liquid left in the steaming steamer after steaming, concentrate it as it is or if it is more than 1/2 concentrated, re-absorb the dried medicine as it is. Rebuilt to be.

Table 2. Herbal Composition and Mixing Ratio of Herbal Hematopoietic Agents (w / w%)

Herbal Medicine Composition Party ear Celestial palace White peony Mixing ratio One One One

The herbal hematopoietic agent was then ground to a mesh size of 4.0 to 2.0 mm in a mill.

In addition, green tea was immediately removed from the leaves in the morning of the day to maintain the green tea's unique ingredients and fresh taste and aroma. After harvesting the first two leaves or first three leaves as the first water teas in May, the tea leaves were made of steamed tea (5% water content) based on the standard process of steamed teas. The green tea material was then ground in a mill to a mesh size of 4.0-2.0 mm.

Green tea powder to the herbal hematopoietic powder obtained above was quantified in a ratio of herbal hematopoietic: green tea = 6: 4 in dry weight ratio (w / w%) to obtain the powdered green tea herbal hematopoietic agent required.

Brown rice was steamed for 1 hour at 100 ° C. immediately after scouring and washing to make a spicy rice, spread out on a dryer foot, dried at 80 ° C. for 3 to 4 hours, and pretreated. Subsequently, the temperature of the cauldron for quenching was set to 100 to 180 ° C., and roasted until light yellow was obtained so as not to burn the pretreated brown rice. Roasted brown rice was pulverized to a mesh size of 4.0 ~ 2.0㎜ in a mill to obtain brown rice powder. Brown rice powder was prepared by quantifying the dry weight ratio (W / W%) of 2: 1 with respect to green tea herbal blood.

Brown rice, which has undergone pre-treatment such as roasting, has a strong taste and blends well with other powdery mixtures.It has good loosening and leachability for hot water and is a good ingredient to be used as a flavor enhancer in canned beverages. Can be

When extracting the prepared green tea, herbal hematopoietic agent and brown rice, based on the total amount of 200g (w / v) of the extract, the number of hot water to be extracted is 17 ~ 22ℓ. Extraction method is a primary extraction method by mixing the powdered herbal hematopoietic agent and brown rice, 12-15 L of ion-exchange resin is added, stirring and extraction at 100 ° C for 30 minutes to 1 hour (adjusted according to the extraction amount) After centrifugation at filtration. The second extraction is carried out in the same way as the first extraction, but the powdered green tea is added with 5 ~ 7 L of hot water with constant temperature of 75 ~ 85 ℃ and stirred for 20 ~ 30 minutes (adjusted according to the extraction amount). After extraction, the mixture was centrifuged and filtered. The primary and secondary extracts are cooled to about 20 ° C. using a coolant, filtered through a vacuum filter, and mixed. Vitamin C 0.02 ~ 0.04% (w / v%) and liquid flavor 0.02 ~ 0.06% (v / v%) are added to the mixed extract, heated to 90 ± 5 ℃ in a planar heat exchanger, and then the can is hot. Charged. Nitrogen gas was injected into the headspace of the filled can to reduce the amount of dissolved oxygen in the product, and then the lid was sealed. The sealed cans are sterilized and cooled to form liquid hematopoietic green tea for canned beverages.

Test Example 1

Evaluation of the general ingredient content, color and sensory evaluation between the green tea, herbal hematopoietic agent, green tea herbal hematopoietic agent obtained by Example 1 by a general method known in the art, and the results are shown in Tables 3, 4 and 5.

Table 3. Contents of General Components of Green Tea, Herbal Hematopoietic Agent, and Green Tea Herbal Hematopoietic Agent

Survey Details moisture(%) Tannins (%) Total Free Amino Acid (mg / 100g) Caffeine(%) Total nitrogen (%) Crude fat (%) Chlorophyll (mg / 100g) % Viewed Crude fiber (%) Energy (㎈ / g) green tea 6.45 14.18 2737.4 1.64 3.12 2.75 229.3 4.83 7.88 4655.1 Herbal Hematopoietic Agent 8.22 1.00 1554.6 0 1.44 5.16 8.4 4.22 6.10 3765.3 Green Tea Herbal Hematopoietic Agent 7.26 6.17 1941.9 0.54 1.78 3.82 129.4 4.37 7.19 4261.8

Table 4. Color Test (Powder Status) of Green Tea, Oriental Hematopoietic Agents and Green Tea Oriental Hematopoietic Agents

Chromaticity (Hunter value) ^* L a b b / a green tea 28.57 -7.5 9.18 -1.21 11.90 Herbal Hematopoietic Agent 45.62 2.91 11.98 4.12 12.33 Green Tea Herbal Hematopoietic Agent 36.73 -2.90 10.53 -3.63 10.92

* Chromaticity (Hunter value): 1. L, a, b values are actual values for indicating color and saturation.

2. b / a: color display. 3. : Saturation display.

Table 5. Sensory Evaluation Results of Green Tea, Oriental Hematopoietic Agents and Green Tea Oriental Hematopoietic Agents

Survey Item Whole incense Overall taste Overall flavor rating ^* green tea 25.0 35.0 60.0 Herbal Hematopoietic Agent 40.1 38.5 78.6 Green Tea Herbal Hematopoietic Agent 42.5 43.0 85.5

* The total score is based on 50 points for total flavors and 50 points for total flavors.

As shown in Tables 3, 4, and 5, the green tea herbal hematopoietic agent obtained according to the present invention is supplemented with ingredients, chromatic complements, and various flavors only in tea leaves or herbal hematopoietic agents, as shown in the above-mentioned components, color, and sensory evaluation results. The overall flavor was elevated but the smell of Chinese medicine was strong.

Test Example 2

In order to investigate whether the green tea herbal hematopoietic formulated with green tea powder in the herbal hematopoietic agent has a hematopoietic effect, the hematopoietic effect was searched by inducing anemia in a test animal. The hematopoietic diet prepared by adding% to the normal diet was prepared and administered to the experimental animal rats by oral administration. Experimental animals were conducted in Sprague-Dawley rats, and the experimental method was used to investigate the body weight and hematopoietic effects before and after bleeding using bleeding method to induce artificial ischemic symptoms. Green tea was extracted from distilled water at 75 ° C. for 3 minutes, and herbal medicines were extracted from boiling water for about 3 hours. The extract was filtered through Whatman filter paper No 41 and freeze-dried to form a powder. The water-soluble substance in powder form of green tea and medicinal herbs thus obtained was mixed and administered to 1% in the normal diet, wherein the ratio of green tea and medicinal herbs was 4: 6. In the experimental rats, the diet containing green tea and medicinal herbs was taken for 4 days before bleeding, and the diet containing green tea and medicinal herbs was taken for 7 days after bleeding. The bleeding method and the amount of bleeding were taken from the tail vein from 100 ml of body weight after 4 days of diet containing green tea and medicinal herbs, which is equivalent to 20% of the total blood volume. Measurements include body weight gain, food intake and hematopoietic index, including red blood cell count, hemoglobin concentration, hematocrit, and reticulocyte count. ) And restorative formation was observed.

The statistical significance of the results obtained in the experiment was analyzed using the SPSS computer program. The mean and standard error of each group were calculated. The statistical significance of the difference between the groups was ANOVA, and the difference was verified by LSD test at P <0.05 level.

The results of animal experiments on the hematopoietic effect of the green tea herbal hematopoietic agent obtained by the present invention are as follows.

In Table 6, the dietary intake after bleeding was significantly increased for 4 days before bleeding, compared to the control group, but after bleeding, there was no increase in dietary intake. Weight gain was also slightly increased compared to the control group, but it was not significant. Therefore, the effect of green tea herbal hematopoiesis on dietary intake, erythrocyte concentration, hemoglobin concentration, erythrocyte volume percentage, and reticulocyte count is insignificant. It was considered to be absent.

Table 1 shows the effect on the hematopoietic effect. After 1 day of bleeding, the green tea herbal hematopoietic agent (hematopoietic tea) induced an increase in erythrocyte count, hemoglobin concentration, and erythrocyte volume percentage with other single treatment groups compared with the control group, but affected the reticulocyte count. Did not. Even after 3 days of bleeding, green tea herbal blood cells induced significant increase in erythrocyte count, hemoglobin concentration and erythrocyte volume percentage compared to other treatment groups. It was recognized that the hemoglobin maintained a significant increase after 7 days, in particular, a sustained and excellent effect on hemoglobin. From these results, hematopoietic car was evaluated to be excellent in ischemia treatment and hematopoietic function.

As a result, the erythrocyte production rate and hemoglobin production rate were followed by Samul-tang and Baek-jak, followed by Baek-jak, Samul-tang, Cheon-gung-seon, and reticulocyte production rate was the best. The whole hematopoietic tea was recognized as a hematopoietic effect compared to the non-treated treatment, and the most significant hematopoietic effect was Samul-tang, followed by Baekjak and Cheongung-soon. Therefore, it was evaluated that the effect of the combination treatment is better than the effect of hematopoietic tea single treatment.

Table 6. Effect of Green Tea Herbal Hematopoietic Agents on Body Weight and Dietary Intake Before and After Bleeding

P Control Green tea + Sookjihwang Green tea + sugar ear Green Tea + Cheongung Green tea + Earl Green Tea Complex Hematopoietic Agent n 5 5 5 5 5 5 Weight on the day of bleeding 214 ± 3 216 ± 3 220 ± 4 221 ± 4 215 ± 6 215 ± 1.0 3 days after bleeding 12.6 ± 2.1 15.9 ± 0.7 16.9 ± 2.7 16.8 ± 1.8 17.2 ± 2.3 14.9 ± 1.5 Weight gain from 3 to 7 days after bleeding 19.9 ± 1.5 24.7 ± 1.6 ^* 29.1 ± 0.8 ^* 25.5 ± 2.2 ^* 22.9 ± 1.7 23.4 ± 1.3 Average weight gain after bleeding 16.3 ± 1.8 20.3 ± 1.2 23.0 ± 1.8 21.2 ± 2 20.1 ± 2 19.2 ± 1.4 Dietary Intake for 4 Days Before Bleeding 57.3 ± 11.2 74.7 ± 3.3 75.1 ± 2.0 ^* 75.3 ± 2.2 ^* 78.0 ± 8.6 ^* 76.2 ± 2.0 ^＊ Dietary intake from the day of bleeding to 3 days after bleeding 39.5 ± 4.1 47.3 ± 1.4 55.7 ± 6.6 ^* 47.5 ± 3.6 48.8 ± 5.0 46.2 ± 1.8 Dietary intake from 4 days to 7 days after bleeding 60.6 ± 1.0 72.1 ± 2.4 86.5 ± 8.3 ^* 72.1 ± 3.0 58.2 ± 9.5 68.6 ± 1.8 Average Dietary Intake 52.5 ± 5.4 97.1 ± 2.4 72.4 ± 5.6 65.0 ± 2.9 61.7 ± 7.7 63.7 ± 1.9

＊ p <0.05, vs control

Table 7. Effect of Green Tea Herbal Hematopoietic Agents on Erythrocyte Count, Hemoglobin Concentration, Erythrocyte Volume Percentage, and Reticulocyte Count

Survey Content Control Green tea + Sookjihwang Green tea + donkey Green Tea + Cheongung Green tea + Earl Green Tea Complex Hematopoietic Agent increase(%) n 5 5 5 5 5 5 - Erythrocyte count (× 10 ⁴ / μL) 1 day after bleeding 5.61 ± 0.24 6.02 ± 0.12 5.83 ± 0.23 6.13 ± 0.12 ^* 6.30 ± 0.11 ^* 6.39 ± 0.08 ＊ +23.8 3 days after bleeding 5.71 ± 0.25 6.08 ± 0.14 5.91 ± 0.19 5.89 ± 0.07 5.91 ± 0.10 6.27 ± 0.06 ＊ +9.8 7 days after bleeding 6.04 ± 0.10 6.04 ± 0.12 6.32 ± 0.14 6.32 ± 0.08 6.69 ± 0.22 ^* 6.39 ± 0.14 +5.8 Total average 5.79 ± 0.2 6.2 ± 0.1 6.0 ± 0.2 6.1 ± 0.1 6.3 ± 0.1 6.35 ± 0.1 +9.7 Hemoglobin concentration (g / dL) 1 day after bleeding 11.6 ± 0.6 12.4 ± 0.1 12.1 ± 0.5 13.5 ± 0.2 ^* 14.1 ± 0.3 ^* 14.6 ± 0.2 +25.9 3 days after bleeding 12.1 ± 0.4 12.5 ± 0.3 12.8 ± 0.1 ^* 12.9 ± 0.2 ^* 13.0 ± 0.2 ^* 13.2 ± 0.2 +9.1 7 days after bleeding 13.0 ± 0.2 14.0 ± 0.3 ^* 14.1 ± 0.3 ^* 14.2 ± 0.2 ^* 14.3 ± 0.2 ^* 14.2 ± 0.3 ＊ +9.2 Total average 12.2 ± 0.4 13.0 ± 0.23 13.0 ± 0.3 13.5 ± 0.2 13.8 ± 0.2 14.0 ± 0.2 +14.8 Erythrocyte volume percentage (%) 1 day after bleeding 33.3 ± 1.8 35.5 ± 0.5 34.7 ± 1.4 37.4 ± 0.6 ^* 37.7 ± 0.5 ^* 37.9 ± 0.7 +13.8 3 days after bleeding 34.4 ± 1.7 36.9 ± 0.8 35.9 ± 1.1 36.8 ± 0.3 36.2 ± 0.8 37.8 ± 0.5 ＊ +9.9 7 days after bleeding 35.2 ± 0.9 38.3 ± 1.0 ^* 38.1 ± 1.2 ^* 38.8 ± 0.8 ^* 39.8 ± 0.5 ^* 37.8 ± 1.0 +7.4 Total average 34.3 ± 1.5 36.9 ± 0.8 36.2 ± 1.2 37.7 ± 0.6 37.9 ± 0.6 37.8 ± 0.7 +10.2 Reticulocyte count (/ 1,000 RBC) 1 day after bleeding 20.5 ± 2.9 28.0 ± 2.3 26.6 ± 4.4 26.3 ± 5.3 23.8 ± 3.8 23.8 ± 6.3 +16.1 3 days after bleeding 28.0 ± 3.0 39.5 ± 7.5 43.0 ± 8.0 47.9 ± 6.1 ^* 42.8 ± 7.3 30.5 ± 4.6 +8.9 7 days after bleeding 17.8 ± 2.4 31.5 ± 1.8 ^* 30.5 ± 5.5 30.9 ± 4.7 ^* 28.7 ± 5.4 25.9 ± 5.1 +45.5 Total average 22.1 ± 2.8 33.0 ± 3.9 33.4 ± 6.0 35.0 ± 5.4 31.8 ± 5.5 26.7 ± 5.3 +20.8

＊ p <0.05 vs control

※ Change (%): [(Control + Hematopoietic) / Control] × 100-100; Increase (+), decrease (-)

In general, the polyphenols of tea, together with theaflavins, have the effect of inhibiting platelet aggregation, preventing thrombosis, ghost suppression of erythrocytes, and important pharmacological agents such as blood pressure lowering agents as antagonists of Ca ²⁺ . It is known to work. Green tea has been reported to have higher catechin component than fermented tea and has better suppression of lipid peroxidation, which effectively suppresses the decrease of α-tocopherol in plasma and red blood cells, thereby reducing the lipids of body fat and liver fat and improving liver function. . Similar effects have been reported to inhibit the vitamin E reduction in Angelica in the present invention, the blood and blood metabolism function of vitamin B ₁₂ contained in Angelica and green tea, blood donation of the uterus and liver function detoxification and blood detoxification It is known to be effective in protecting liver, preventing hepatic glycogen degradation and treating chronic hepatitis. The antioxidant effect of the green tea and Angelica as described above is synergistic effect when coexisted with tocopherol, vitamin C, caffeine and the like, and the effect is increased and antagonistic action with hematopoietic drugs such as Angelica, Countess, hematopoietic Similar results have been shown in animal studies on tea efficacy. Significantly increased RBC, hemoglobin, and red blood cell percentage compared to the control group, and the increase in the number of reticulocytes that appear only after bleeding as hemoglobin-synthesizing red blood cells demonstrate the above effects. As the components interact with the hematopoietic components of Chinese medicine, it is interpreted that the antagonistic synergism and antioxidant activity are increased, thereby enhancing the efficacy. The basis for this interpretation is that green tea herbal hematopoietic agent contains 30 to 40% of the amount of polyphenol-based tannins, and the caffeine component is 0.54%, as shown in the component content of Table 3 in Test Example 1. I contain it.

Therefore, the green tea herbal hematopoietic agent of the present invention has an excellent effect on hematopoiesis and hematopoiesis according to the results of the animal experiments. It is interpreted that the pharmacological antagonism, synergism, activation and antioxidant activity, and the whole body metabolism function of the herbal medicine performed hemostasis and bodhisattva, showing various actions on tissue and bone marrow.

Green tea herbal hematopoietic agent of the present invention has a greater overall hematopoietic effect than the control group, and thus, green tea and herbal medicines have a detoxification and neutralization effect, and thus, long-term drinking is possible. .

Green tea herbal hematopoiesis prepared according to the method of the present invention has a hematopoietic efficacy and palatability as a result of animal experiments, and is a functional tea for hematopoiesis containing various efficacy and ingredients in terms of nutrition, bioregulation and pharmacology. Can contribute.

Test Example 3

For the hematopoietic green tea for drink obtained in Example 2, 30 men and women were given preference ratings by morning and afternoon. Scoring was done using a 9-level symbol scale, which was scored from one point of "I hate the best" to nine points of "best." The results of the evaluation are shown in Table 9. A product is a pure canned hematopoietic tea product without flavor enhancer and B product is a canned hematopoietic tea product with added flavor enhancer. Expressed as a rating.

Table 9. Canned Hematopoietic Tea A and Gourmet Hematopoietic Tea B Grade Scores

score system morning afternoon south female south female A B A B A B A B A B It is very good Very good Normally It is slightly good It is not good or hates It is slightly dislikes. It is usually dislikes. It is dislikes very much. X987654321 f _A 0144106410 f _B 12124200000 f _A 005686320 f _B 11154000000 f _A 011569530 f _B 14133000000 f _A 004697310 f _B 14151000000 f _A 02142133281570 f _B 515512200000 system 30 30 30 30 30 30 30 30 120 120

According to the results shown in Table 9, both males and females were evaluated to have excellent flavor preference in the added B product compared to the A product without the flavor enhancer.

The hematopoietic green tea for the canned beverage obtained by the above method is well stored and processed by the processing of green tea herbal blood, which is the basic material of the canned beverage, and it is possible to extract hot water immediately. In particular, the leaching in hot water during processing and the leaching time are short, so the process time can be shortened and beverages with uniform quality can be obtained. And since the processed product is reinforced with vitamin C ingredient that acts as anti-oxidant and synergist, it can be expected to improve the shelf life and excellent hematopoietic effect of the product, and the strong smell of Chinese medicine is alleviated by the addition of brown rice's delicious taste and food flavoring. The soft and delicious flavor is increased, resulting in a tea with excellent functionality and palatability.

As such, the present invention may improve the palatability as well as pharmacology by mixing green tea and brown rice in the herbal medicine.

In addition, while maintaining the efficacy of the green tea and the medicinal herb, as well as increasing the pharmacological synergism by the interaction of green tea and herbal medicine, not only increases the hematopoietic efficacy, but also uses only natural ingredients of green tea and Chinese medicine, side effects and toxicity In general, it can be consumed like ordinary foods in general, so it can contribute to public health by supplementing and preventing ischemia.

Claims (3)

Green tea herbal hematopoietic agent consisting of 60 to 80% by weight of herbal hematopoietic agent and 25 to 50 parts by weight of Angelica 25-50 parts by weight, 25 to 50 parts by weight and Baekjak and 20 to 40% by weight of green tea and dry brown rice Hematopoietic green tea blended 4: 1. A first step of combining the Angelica, Cheongung, Baekjak with 25 to 50 parts by weight, steaming, drying and powdering to obtain a herbal hematopoietic agent, The herbal hematopoietic agent obtained in the first step and the green tea powder were quantified in a ratio of 6: 4 to 8: 2 to make unmixed green tea herbal hematopoietic agent, steamed and then roasted brown rice powder at 100 to 180 ° C. to the green tea herbal hematopoietic agent. A second step of quantifying the dry weight ratio so as to be 2: 1 to 4: 1, Extraction of the primary hematopoietic agent and brown rice powder quantified in the second step, and the primary extract and secondary extract obtained by the secondary extraction filtration of green tea under reduced pressure filtration, and then mixed, and vitamin C 0.02 ~ 0.04% ( w / v%) and a method for producing a liquid hematopoietic green tea for canned drinks, comprising a third step of adding a liquid chemical 0.02 to 0.06% (v / v%). According to claim 3, wherein the first extraction is herbal hematopoietic agent and brown rice extracted for 30 minutes to 1 hour at 100 ℃, the second extraction only green tea extracted at 75 to 85 ℃ 20 to 30 minutes, the total amount of the extraction When 200g of the amount of hot water to be extracted is a method for producing liquid hematopoietic green tea for canned beverages based on 17 to 22ℓ.