KR20020010119A - 식물 리파제를 엔코딩하는 디엔에이, 형질전환된 식물과식물 노화를 조절하기 위한 방법 - Google Patents
식물 리파제를 엔코딩하는 디엔에이, 형질전환된 식물과식물 노화를 조절하기 위한 방법 Download PDFInfo
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- KR20020010119A KR20020010119A KR1020017010405A KR20017010405A KR20020010119A KR 20020010119 A KR20020010119 A KR 20020010119A KR 1020017010405 A KR1020017010405 A KR 1020017010405A KR 20017010405 A KR20017010405 A KR 20017010405A KR 20020010119 A KR20020010119 A KR 20020010119A
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- aging
- plant
- lipase
- polynucleotide
- dna molecule
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- C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
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- C12N15/8249—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving ethylene biosynthesis, senescence or fruit development, e.g. modified tomato ripening, cut flower shelf-life
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
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- Immunology (AREA)
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- Chemical Kinetics & Catalysis (AREA)
- Nutrition Science (AREA)
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US25028099A | 1999-02-16 | 1999-02-16 | |
| US09/250,280 | 1999-02-16 | ||
| PCT/US2000/003494 WO2000049164A1 (en) | 1999-02-16 | 2000-02-14 | Dna encoding a plant lipase, transgenic plants and a method for controlling senescence in plants |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20020010119A true KR20020010119A (ko) | 2002-02-02 |
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| JP (1) | JP4885359B2 (https=) |
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| CA (1) | CA2362546C (https=) |
| DE (1) | DE60024971D1 (https=) |
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| NZ (1) | NZ514123A (https=) |
| UA (1) | UA85528C2 (https=) |
| WO (1) | WO2000049164A1 (https=) |
Families Citing this family (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6936289B2 (en) | 1995-06-07 | 2005-08-30 | Danisco A/S | Method of improving the properties of a flour dough, a flour dough improving composition and improved food products |
| ES2168236T3 (es) | 1997-04-09 | 2005-04-16 | Danisco A/S | Utilizacion de lipasa para mejorar las pastas de pan y los productos de panaderia. |
| AU778437B2 (en) | 1999-02-16 | 2004-12-02 | Senesco, Inc. | DNA encoding a plant lipase, transgenic plants and a method for controlling senescence in plants |
| NZ523280A (en) * | 2000-06-19 | 2005-02-25 | Senesco Technologies Inc | DNA encoding a plant lipase, transgenic plants and a method for controlling senescence in plants |
| AUPQ994600A0 (en) | 2000-09-06 | 2000-09-28 | Agriculture Victoria Services Pty Ltd | Manipulation of plant senescene |
| BR0209154A (pt) | 2001-05-18 | 2004-07-20 | Danisco | Processo de preparação de uma massa com uma enzima |
| DE602004030000D1 (de) | 2003-01-17 | 2010-12-23 | Danisco | Verfahren zur in-situ-herstellung eines emulgators in einem nahrungsmittel |
| CN102210376B (zh) * | 2003-03-10 | 2014-12-31 | 波伊特研究股份有限公司 | 利用生淀粉生产乙醇的方法 |
| US20050233030A1 (en) * | 2004-03-10 | 2005-10-20 | Broin And Associates, Inc. | Methods and systems for producing ethanol using raw starch and fractionation |
| US20050239181A1 (en) * | 2004-03-10 | 2005-10-27 | Broin And Associates, Inc. | Continuous process for producing ethanol using raw starch |
| GB0405637D0 (en) | 2004-03-12 | 2004-04-21 | Danisco | Protein |
| CN101052702B (zh) | 2004-07-16 | 2013-01-09 | 杜邦营养生物科学有限公司 | 脂肪分解酶及其在食品工业中的应用 |
| US20070037267A1 (en) * | 2005-05-02 | 2007-02-15 | Broin And Associates, Inc. | Methods and systems for producing ethanol using raw starch and fractionation |
| US7919289B2 (en) | 2005-10-10 | 2011-04-05 | Poet Research, Inc. | Methods and systems for producing ethanol using raw starch and selecting plant material |
| US7309602B2 (en) * | 2006-04-13 | 2007-12-18 | Ambrozea, Inc. | Compositions and methods for producing fermentation products and residuals |
| US20070244719A1 (en) * | 2006-04-13 | 2007-10-18 | David Peter R | Compositions and methods for producing fermentation products and residuals |
| US20070243235A1 (en) * | 2006-04-13 | 2007-10-18 | David Peter R | Compositions and methods for producing fermentation products and residuals |
| CN101680035B (zh) | 2007-03-28 | 2017-05-31 | 孟山都技术有限公司 | 与主要大豆植物成熟期和生长习性基因组区域相关的snp标记的效用 |
| CN101815433B (zh) | 2007-09-11 | 2013-06-05 | 孟山都技术公司 | α-优化的β-伴大豆球蛋白增高的大豆 |
| US8450094B1 (en) | 2009-03-03 | 2013-05-28 | Poet Research, Inc. | System for management of yeast to facilitate the production of ethanol |
| MX2011009269A (es) | 2009-03-03 | 2011-09-26 | Poet Res Inc | Fermentacion de biomasa para la produccion de etanol. |
| WO2010102060A2 (en) * | 2009-03-03 | 2010-09-10 | Poet Research, Inc. | System for pre-treatment of biomass for the production of ethanol |
| US9068206B1 (en) | 2009-03-03 | 2015-06-30 | Poet Research, Inc. | System for treatment of biomass to facilitate the production of ethanol |
| US8839078B2 (en) * | 2010-03-05 | 2014-09-16 | Samsung Electronics Co., Ltd. | Application layer FEC framework for WiGig |
| WO2014041116A1 (en) | 2012-09-17 | 2014-03-20 | F. Hoffmann-La Roche Ag | Method for the production of polypeptides in the periplasm of prokaryotic cells |
| EP2958713B1 (en) | 2013-02-20 | 2019-05-08 | Benchmade Knife Co., Inc. | Folding knife with dual operational modes |
| WO2019055431A1 (en) * | 2017-09-12 | 2019-03-21 | The Regents Of The University Of California | PEPTIDE INHIBITORS OF INSULIN DEGRADATION ENZYME |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9318927D0 (en) * | 1993-09-13 | 1993-10-27 | Zeneca Ltd | Regulation of senescence |
| FR2722798B1 (fr) | 1994-07-25 | 1996-09-13 | Toulouse Inst Nat Polytech | 1procede de production d'acides gras2plantes oleagineuses |
| US5689042A (en) | 1995-03-29 | 1997-11-18 | Wisconsin Alumni Research Foundation | Transgenic plants with altered senescence characteristics |
| AUPN286295A0 (en) * | 1995-05-09 | 1995-07-06 | Allrad No.1 Pty Ltd | Transgenic carnations exhibit prolonged post-harvest life |
| WO1997013851A1 (en) * | 1995-10-13 | 1997-04-17 | Purdue Research Foundation | Improvement of fruit quality by inhibiting production of lipoxygenase in fruits |
| US5824875A (en) | 1996-10-01 | 1998-10-20 | Colorado State University Through Its Agent Colorado State University Research Foundation | 1-aminocyclopropane-1-carboxylate synthase genes from pelargonium |
| GB9817909D0 (en) | 1998-08-17 | 1998-10-14 | Zeneca Ltd | DNA constructs |
| AU778437B2 (en) | 1999-02-16 | 2004-12-02 | Senesco, Inc. | DNA encoding a plant lipase, transgenic plants and a method for controlling senescence in plants |
| EP1033405A3 (en) | 1999-02-25 | 2001-08-01 | Ceres Incorporated | Sequence-determined DNA fragments and corresponding polypeptides encoded thereby |
-
2000
- 2000-02-14 AU AU35942/00A patent/AU778437B2/en not_active Ceased
- 2000-02-14 KR KR1020017010405A patent/KR20020010119A/ko not_active Ceased
- 2000-02-14 JP JP2000599888A patent/JP4885359B2/ja not_active Expired - Fee Related
- 2000-02-14 AT AT00914569T patent/ATE313636T1/de not_active IP Right Cessation
- 2000-02-14 NZ NZ514123A patent/NZ514123A/en not_active IP Right Cessation
- 2000-02-14 CN CN00806296A patent/CN1354797A/zh active Pending
- 2000-02-14 WO PCT/US2000/003494 patent/WO2000049164A1/en not_active Ceased
- 2000-02-14 IL IL14483500A patent/IL144835A0/xx unknown
- 2000-02-14 DE DE60024971T patent/DE60024971D1/de not_active Expired - Lifetime
- 2000-02-14 CA CA2362546A patent/CA2362546C/en not_active Expired - Fee Related
- 2000-02-14 HK HK02108970.5A patent/HK1047299A1/zh unknown
- 2000-02-14 EP EP00914569A patent/EP1155134B1/en not_active Expired - Lifetime
- 2000-07-05 US US09/610,104 patent/US6774284B1/en not_active Expired - Fee Related
-
2001
- 2001-06-19 UA UA2003010421A patent/UA85528C2/ru unknown
- 2001-08-09 IL IL144835A patent/IL144835A/en not_active IP Right Cessation
-
2003
- 2003-10-01 US US10/674,540 patent/US7087419B2/en not_active Expired - Fee Related
-
2006
- 2006-07-14 US US11/486,250 patent/US20070011778A1/en not_active Abandoned
-
2007
- 2007-10-10 US US11/870,229 patent/US20080039335A1/en not_active Abandoned
-
2011
- 2011-04-04 US US13/079,563 patent/US20110196143A1/en not_active Abandoned
-
2012
- 2012-07-18 US US13/551,847 patent/US20130184449A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20130184449A1 (en) | 2013-07-18 |
| US20080039335A1 (en) | 2008-02-14 |
| AU778437B2 (en) | 2004-12-02 |
| US7087419B2 (en) | 2006-08-08 |
| UA85528C2 (ru) | 2009-02-10 |
| NZ514123A (en) | 2003-10-31 |
| DE60024971D1 (de) | 2006-01-26 |
| JP4885359B2 (ja) | 2012-02-29 |
| EP1155134A1 (en) | 2001-11-21 |
| US20110196143A1 (en) | 2011-08-11 |
| AU3594200A (en) | 2000-09-04 |
| WO2000049164A9 (en) | 2002-04-11 |
| US20040158891A1 (en) | 2004-08-12 |
| CA2362546A1 (en) | 2000-08-24 |
| HK1047299A1 (zh) | 2003-02-14 |
| ATE313636T1 (de) | 2006-01-15 |
| EP1155134B1 (en) | 2005-12-21 |
| WO2000049164A1 (en) | 2000-08-24 |
| US6774284B1 (en) | 2004-08-10 |
| JP2002536979A (ja) | 2002-11-05 |
| CN1354797A (zh) | 2002-06-19 |
| IL144835A (en) | 2014-07-31 |
| US20070011778A1 (en) | 2007-01-11 |
| IL144835A0 (en) | 2002-06-30 |
| CA2362546C (en) | 2012-05-15 |
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