KR20010069333A - Manufacturing method of microbial preparation for fermentation - Google Patents
Manufacturing method of microbial preparation for fermentation Download PDFInfo
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Abstract
Description
본 발명은 느타리버섯 재배 시 사용되는 배지의 발효에 유용한 미생물 제재의 제조방법에 관한 것으로서, 좀 더 상세하게는 현재 행하여지고 있는 자연퇴적발효과정 중에 문제가 되고 있는 유해성 곰팡이 및 혐기성세균에 의한 오염을 방지하고 버섯균사의 증식촉진 및 자실체의 수량증대를 목적으로 효소분해활성 및 느타리버섯 증식촉진효과가 우수한 미생물의 조합으로 고초균(Bacillussp.), 효모(Saccharomycessp.), 방선균(Streptomycessp.), 광합성세균(Photosynthetic bacteria) 및 고온성방선균(Thermoactinomyces) 등 5종의 미생물을 각각의 증식에 유리한 배양조건, 배지조성으로 준비한 액체배지에서 3∼6일간 배양한 후, 팽연왕겨, wood chip 등의 다공성 식물소재에 균체를 담지시겨 혼합, 건조한 느타리버섯 배지발효용 미생물제재의 제조방법에 관한 것이다.The present invention relates to a method for producing a microbial agent useful for fermentation of a medium used for cultivating oyster mushrooms, and more particularly, to prevent contamination by harmful fungi and anaerobic bacteria, which are a problem in the present natural sedimentation effect tablets. Bacillus sp., Saccharomyces sp., Streptomyces sp. Are a combination of microorganisms with excellent enzyme degrading activity and oyster mushroom growth promoting effect for the purpose of preventing and promoting the growth of mushroom mycelia and increasing the yield of fruiting bodies. , photosynthetic bacteria (photosynthetic bacteria) and thermophilic actinomycetes (Thermoactinomyces) including five species of microorganisms favorable culture conditions for each of the proliferation, 3-6 days in liquid medium prepared with the medium composition and incubated, paengyeon rice husk, wood chip, etc. The present invention relates to a method for preparing a microbial material for fermenting mixed and dried oyster mushroom medium by loading microbial cells in a porous plant material.
국내 느타리버섯의 재배농가호수는 1999년 현재 1만 여호가 넘으며 전체 버섯생산량 중 70% 이상을 차지하는 주요작물이다. 배지원료로는 볏짚, 폐솜등 섬유소함유 재료가 사용되고 있으며, 수분함량의 균일화, 연화에 의한 물리화학성의 개선 및 방선균 등 길항성 미생물의 증식에 의한 오염균의 증식억제를 목적으로 10여일간 자연퇴적발효를 실시하고 있다. 느타리버섯배지의 제조는 작물에 대한 우량토양의 역할과 같이 느타리버섯 재배에 가장 큰 영향을 주게되므로 이 발효과정의 적합 여부에 따라 버섯재배의 성공, 실패가 좌우되고 있다.The number of cultivated farm lakes in Korea is more than 10,000 as of 1999, and it is a major crop that accounts for more than 70% of the total mushroom production. Fiber medium-containing materials such as rice straw and waste cotton are used as media raw materials, and they are naturally deposited for 10 days for the purpose of equalizing water content, improving physicochemical properties by softening, and suppressing the growth of contaminating bacteria by proliferation of antagonistic microorganisms such as actinomycetes. Fermentation is carried out. The production of Pleurotus eryngii medium has the greatest effect on cultivation of Pleurotus erythritis, such as the role of good soil for crops, and the success or failure of mushroom cultivation depends on the suitability of this fermentation process.
퇴적발효의 원리는 볏짚, 폐솜 등에 함유된 탄수화물, 당류 및 단백질 등 오염미생물에 의해 쉽게 대사 될 수 있는 화합물을 고갈시키고 고온호기성 발효를 통해 방선균증식에 의한 오염미생물에 대한 길항효과를 증대시켜 느타리버섯균사가 우점적으로 증식 가능한 lignocellulose를 조성해 주는 것이다.The principle of sedimentation fermentation is the depletion of compounds that can be easily metabolized by contaminating microorganisms such as carbohydrates, sugars and proteins contained in rice straw, waste cotton, etc. Mycelium forms a lignocellulose that can proliferate predominantly.
그러나 현재 느타리재배시 사용되는 퇴적발효의 방법을 수작업 또는 경운기, 트랙터 등을 이용하며 물을 가하여 6ton정도의 퇴적더미를 쌓고 10여 일간 자연퇴적발효를 실시함으로서 수분조정이 어렵고, 혼합, 교반의 미흡으로 불완전발효에 의한 오염발생이 심각한 실정으로 배양실패율이 30∼50% 정도에 달해 느타리재배 농가의 수익성 악화는 물론 특용작물로서의 매력을 상실케 하는 주원인이 되고 있다.However, the sediment fermentation method currently used in cultivation is carried out by hand or tiller, tractor, etc. by adding water to accumulate piles of about 6 tons and performing natural sediment fermentation for 10 days. As a result of serious fermentation due to incomplete fermentation, the cultivation failure rate is about 30-50%, which is the main cause of deterioration of profitability of cultivated farmers and loss of special crops.
자연퇴적발효는 백화현상(white lime-like coating)을 지표로 고온호기성발효의 진행여부를 판단하고 있으나 다양한 미생물상(microflora) 및 미생물천이 등에 대한 제어방법이 없어 발효과정 중에 작용하는 유용미생물의 작용에 관한 구체적인 사실은 전혀 알려져 있지 않다. 또한 작업의 용이성으로 폐솜을 선호하여 원료의 90%이상을 수입하고 있는 실정으로 금후 가격의 상승, 조달문제 등이 발생할 소지가 많아 국내의 유휴농산부산물인 톱밥, 볏짚, 주박, 팽연왕겨, 미강 등으로의 대체가 요구되고 있다.Natural sedimentation fermentation is based on white lime-like coating to determine whether high-temperature aerobic fermentation is in progress, but there is no control method for various microflora and microbial transitions. The specific facts about it are not known at all. In addition, they prefer to use waste cotton for ease of operation, and import more than 90% of raw materials.Therefore, there is a high possibility of future price increase and procurement problems.Therefore, domestic idle agricultural by-products such as sawdust, rice straw, sake lees, rice husk, rice bran, etc. Replacement is required.
이런한 자연퇴적발효과정 중의 문제점들을 해결하기 위하여 본 발명자들은 발효과정 중에 유용한 작용을 하며, 균사증식촉진 및 자실체 수량증대를 꾀할 수 있는 미생물들을 연구해온 결과 탄수화물, 단백질 및 섬유소의 분해효소를 분비하고 vitamine, 아미노산 등의 버섯증식촉진 물질을 제공하는 미생물을 분리하였고, 이들의 효과적인 조합을 통해 느타리버섯 배지의 발효 및 버섯증식촉진 목적을 달성할 수 있는 새로운 실험결과를 얻을 수 있었다. 따라서 본 발명의 목적은 느타리버섯배지의 오염방지와 수량증대에 적합한 미생물제재의 제조방법을 제공하는데 있다.In order to solve the problems of spontaneous sedimentation effect, the present inventors have a useful function during the fermentation process, and as a result of studying microorganisms that can promote mycelial growth and increase the yield of fruiting bodies, secreting carbohydrate, protein and fibrinolytic enzymes Microorganisms that provide mushroom growth promoting substances, such as vitamins and amino acids, were isolated and their effective combinations yielded new experimental results to achieve fermentation and mushroom growth promotion objectives of oyster mushroom media. Accordingly, an object of the present invention is to provide a method for preparing a microbial preparation suitable for preventing contamination and increasing yield of oyster mushroom medium.
상기 목적을 달성하기 위한 본 발명의 방법은 중온성 영역에서 탄수화물, 당 및 단백질 등 대사 되기 쉬운 영양원을 유해성 곰팡이류 및 혐기성 세균에 비해 증식속도가 빨라 우점적으로 이용할 수 있는 활성미생물인 고초균, 효모균과 미생물 증식자체가 증식촉진 물질로 작용하는 광합성세균을 이용하고, 중온성방선균 및 고온성방선균의 항생물질 분비기능을 이용하여 유해성 곰팡이류의 발생을 억제하고 섬유소의 분해효소활성을 이용하여 버섯균사의 증식에 유리한 lignocellulose化를 조장하는 것이다.The method of the present invention for achieving the above object is an active microorganism that can be used predominantly because of the rapid growth rate of nutrients such as carbohydrates, sugars and proteins in the mesophilic region compared to harmful fungi and anaerobic bacteria, such as Bacillus subtilis, yeast and By using photosynthetic bacteria where the microbial growth itself acts as a growth-promoting substance, it suppresses the occurrence of harmful fungi by using the antibiotic secretion function of mesophilic actinomycetes and thermophilic actinomycetes, and uses the degrading enzyme activity of fibrin to proliferate mushroom mycelia. It is to promote lignocellulose that is beneficial to.
따라서 상기 5종의 미생물을 각각의 증식최적조건의 배지조성, 배양 조건으로 준비한 액체, 고체배지에 3∼6일간 배양하고 팽연왕겨, wood chip등의 다공성 식물소재의 담체에 균체를 담지시켜 50℃이하의 온도에서 통풍건조시킨 분말인 미생물제재를 퇴적발효 시 첨가해 줌으로서 기존의 발효과정중에 발생하고 있는 오염의 방지와 버섯수량 증대에 유용하게 쓰일 수 있다.Therefore, the five microorganisms were cultured for 3 to 6 days in a medium and liquid medium prepared for optimal growth conditions and culture conditions for each growth condition, and the microorganisms were supported on a carrier of porous plant material such as bulrush chaff and wood chips to 50 ° C. By adding a microbial material, which is a powder dried at the following temperature during deposition and fermentation, it can be useful for preventing the contamination occurring during the existing fermentation process and increasing the amount of mushrooms.
이하 본 발명의 방법을 좀 더 구체적으로 살펴보면 다음과 같다.Hereinafter, the method of the present invention will be described in more detail.
본 발명에 사용되는 중온성미생물은 30℃부근의 온도영역에서 amylase, protease등의 효소활성을 지닌 고초균(Bacillus subtilis)과 항생 물질을 분비하여 느타리버섯배지의 주오염균인Tricodermasp., 곰팡이류에 대한 길항작용을 지닌 방선균(Streptomyces griceus) 및 균체의 성분 중 vitamine, 아미노산의 함유량이 높아 느타리버섯균사 및 자실체의 촉진물질로 이용될 수 있는 효모 (Saccharomyces cerevisiae), 광합성세균(Rhodobacter)로 구성되며, 증식력이 우수한 특성이 있다. 또한 고온성미생물로는 50∼55℃부근의 온도에서 섬유소분해활성을 지닌 방선균(Thermoactinomyces)으로서 이 미생물들을 자연계에서 분리하기 위한 방법으로서는 山里一英 등의 일반적인 미생물분리법(微生物の分離法 1986, R&D planning)으로 간단히 분리 ·동정하였다.A mesophilic microorganism which secrete the Bacillus subtilis (Bacillus subtilis) and antibiotics, with the enzyme activity, such as amylase, protease at a temperature region in the vicinity of 30 ℃ main contaminants of Pleurotus medium Tricoderma sp., Fungi used in the present invention It consists of Streptomyces griceus , which has an antagonistic action against it , and yeast ( Saccharomyces cerevisiae ) and photosynthetic bacterium ( Rhodobacter ), which can be used as a stimulant for Pleurotus mycelia and fruiting bodies because of its high content of vitamine and amino acids It has excellent properties of proliferation. In addition, high-temperature microorganisms are thermoactinomyces having fibrinolytic activity at a temperature near 50 to 55 ° C. As a method for separating these microorganisms in nature, a general microbial separation method such as Sanrio Ibe (1986, R & D) Simple separation and identification by planning).
또한 이들 선택된 균들은 유전자원으로서 KAIST유전자은행, 한국종균협회등 국내 균주보존기관에서 보존되고 있는 미생물들로서 자유롭게 입수가 가능하며 각각의 효소활성 및 균종을 확인한 후에 사용하였다.In addition, these selected organisms are freely available as microorganisms preserved in domestic strain preservation institutions such as KAIST Gene Bank, Korea Spawn Association, etc. and used after confirming their respective enzyme activity and species.
상기의 활성미생물들을 대량으로 취득하는 기술은 탄소원, 질소원 및 무기원소 등이 함유된 액체배지에 통기, 교반 배양하는 방법이 사용된다.As a technique for acquiring the active microorganisms in large quantities, a method of aeration and stirring culture in a liquid medium containing a carbon source, a nitrogen source, an inorganic element, and the like is used.
탄소원으로는 전분, 포도당 등이 사용되며 질소원으로서는 옥수수 침지액, 대두박 등이, 영양물질로서는 Yeast extract, Malt extract, 기타 염류 등이 사용된다. 따라서 조성된 액체배지에서 적정온도에서 3∼6일간 배양하면 1010cells/㎖ 이상의 고농도 균체배양액을 얻을 수 있다. 또한 고체배양도 조작이 간편하고 경비가 저렴한 이점이 있어 좋은 배양수단의 하나이다. 이 고체배양은 일반적인 코지(국)의 제조방법에 준하면 되고 밀기울, 미강, 곡류등의 배지로 손쉽게 균체배양물을 얻을 수 있다. 상기 배양된 균체는 팽연왕겨, wood chip 등 다공성 식물소재에 담지시켜 50℃ 이하의 온도에서 통풍·건조하여 수분함량을 13% 이하로 조정하면 장시 보존에도 안정한 미생물 제재의 제조가 가능하다.Starch, glucose, etc. are used as carbon sources, corn steep liquor, soybean meal, etc. are used as nitrogen sources, and yeast extract, malt extract, and other salts are used as nutrients. Therefore, high concentration cell culture solution of 10 10 cells / ㎖ can be obtained by incubating for 3 to 6 days at the appropriate temperature in the liquid medium. In addition, solid culture is one of the good culture means because it has the advantage of easy operation and low cost. This solid culture should follow the manufacturing method of a common koji (soy), and cell culture can be easily obtained with a medium such as bran, rice bran, and cereals. The cultured cells are supported on a porous plant material such as bulrush chaff, wood chip and ventilated and dried at a temperature of 50 ° C. or lower to adjust the moisture content to 13% or less, thereby enabling the production of a stable microorganism product even in long-term preservation.
이하 실시 예 및 비교 예를 통하여 본 발명을 좀 더 구체적으로 살펴보지만 하기 예에 본 발명의 범주가 한정되는 것은 아니다.Hereinafter, the present invention will be described in more detail with reference to Examples and Comparative Examples, but the scope of the present invention is not limited to the following Examples.
비교 예 1)Comparative example 1)
면실피 ·톱밥 및 맥주박을 용량(%) 40:40:20의 비율로 혼합하고 수분함량을 65∼70% 조정하기 위해 Mixer를 이용하여 2시간 정도 충분히 교반하였다. 이 혼합된 배지를 폭 2m, 높이 1.5m의 더미형태로 쌓고 상단 30cm 깊이에 봉상의 온도계를 꽂아 품온이 60℃에 도달하면 뒤집기를 실시하여 내 ·외부 및 상하부가 골고루 섞이도록 하였으며 약 10일간 3회 뒤집기를 행한후 발효를 완료하고 40x40x15cm 상자에 배양비닐을 깔고 8kg 정도의 배지를 담아 살균실로 입상하였다. 살균은 65℃에서 10시간 실시하였고 55℃로 하온시킨 후 3일간 1일 1회 환기시키면서 3일간 후발효를 행하여 느타리재배용 발효배지를 제조하였다.Cotton silk and sawdust and beer foil were mixed at a ratio of 40:40:20 in volume (%) and sufficiently stirred for 2 hours using a mixer to adjust the water content of 65 to 70%. The mixed medium was stacked in a pile shape of 2m in width and 1.5m in height, and a rod-shaped thermometer was inserted at a depth of 30cm at the top. When the temperature reached 60 ℃, the inside and outside and the top and bottom were mixed evenly. After inverting the ashes, fermentation was completed and cultured in a 40x40x15cm box and placed in a sterilization chamber containing about 8kg of medium. Sterilization was performed at 65 ° C. for 10 hours, and after fermentation was carried out for 3 days, while lowering the temperature to 55 ° C., once a day for 3 days, to prepare fermentation medium for cultivation.
실시 예 1)Example 1
- 미생물 제재의 제조-Preparation of microbial products
효모(Saccharomyces cerevisiaeKCTC 7245)균을 YM 한천배지에 접종하고 30℃에서 3일간 배양한 후 500㎖ 삼각플라스크에 YM 액체배지 100㎖씩 분주하여 121℃에서 15분간 살균한 후 상기의 YM 한천배지 상의 균체를 1-2 백금이 량씩 접종하여 30℃에서 3일간 배양하였다. 또한 scale up 실험은 30ℓ발효조(Jar fermenter)에 20ℓ의 YM 액체 배지를 살균한 후 동일한 방법으로 배양하였다. 배양의 완료된 균체 배양액은 3℃냉장고에 보관하며 필요시마다 사용하였다.Inoculate yeast ( Saccharomyces cerevisiae KCTC 7245) to YM agar medium, incubate at 30 ° C for 3 days, dispense 100 ml of YM liquid medium into 500 ml Erlenmeyer flask and sterilize for 15 minutes at 121 ° C. Cells were inoculated with 1-2 platinum aliquots and incubated at 30 ° C. for 3 days. In addition, the scale up experiment was sterilized 20 L YM liquid medium in a 30 L fermenter (Jar fermenter) and then cultured in the same manner. Completed cell culture of the culture was stored in a 3 ℃ refrigerator and used whenever necessary.
중온성방선균(Streptomyces grices subsp.griseusKCTC 9135)은 상기의 효모균의 배양방법과 동일하게 YM 배지를 사용하였고 28℃에서 6일간 배양하였다.Mesophilic actinomycetes (Streptomyces grices subsp. Griseus KCTC 9135) was the same as the culture method of the yeast using a YM medium and incubated at 28 ℃ 6 days.
고초균(Bacillus subtilisKCTC 1028)은 Nutrient 배지를 사용하였고 30℃에서 4일간 상기의 방법과 동일하게 배양하였다. Bacillus subtilis KCTC 1028 was used for Nutrient medium and incubated in the same manner as above for 4 days at 30 ℃.
고온성방선균(Thermoactinomyces glaucusKCTC 9645)은 YGM 배지를 사용하였고 50℃에서 6일간 상기와 동일하게 배양하였다. Thermoactinomyces glaucus KCTC 9645 was used for YGM medium and incubated at 50 ° C. for 6 days.
광합성세균(RhodobacterKCTC 1437)은 Van Niel's Yeast 배지를 사용하였고 광을 조사하며 28℃에서 6일간 혐기적으로 배양하였다.Photosynthetic bacteria ( Rhodobacter KCTC 1437) used Van Niel's Yeast medium and was incubated anaerobicly for 6 days at 28 ℃ with light.
상기의 조건으로 배양하여 얻어진 배양액은 각각 배양액 ㎖당 1010cell 이상의 균체를 얻을 수 있었다. 5종의 균체배양액을 용량비 10배의 건조된 팽연왕겨에 동량씩 첨가하고 교반기로 잘 혼합한 후, 50℃ 이하의 온도에서 통풍 ·건조시켜 수분함량이 13% 이하가 되도록 조정하였다.The culture medium obtained by culturing under the above conditions was able to obtain more than 10 10 cells per ml of culture medium. Five cell culture fluids were added in equal amounts to the dried expanded rice husks with a volume ratio of 10 times and mixed well with a stirrer, followed by ventilation and drying at a temperature of 50 ° C. or lower to adjust the water content to 13% or less.
제조된 미생물제재를 비교 예1의 느타리버섯 배지원료에 2%(w/w)의 비율로 골고루 혼합이 되도록 첨가하여 동일한 방법으로 퇴적발효를 실시하며 발효 성적을비교하였다.The prepared microbial material was added to the oyster mushroom medium raw material of Comparative Example 1 to be evenly mixed at a ratio of 2% (w / w), and the fermentation results were compared in the same manner.
미생물제재를 첨가하여 발효시킨 경우, 발효기간이 4일 정도 단축되었으며 미생물이 왕성한 증식을 나타내 총 미생물수도 109으로 높게 나타났고, 혐기성세균의 오염에 의한 악취의 발생도 없었다. 또한 고온호기성발효의 지표인 백화현상도 매우 양호하게 나타나 안정적이고 양호한 발효성적을 얻을 수 있었다.When fermentation with microbial agent was added, the fermentation period was shortened by about 4 days, the growth of microorganisms was high, and the total number of microorganisms was 10 9 , and there was no odor due to contamination of anaerobic bacteria. In addition, the whitening phenomenon, which is an indicator of high temperature aerobic fermentation, was also very good, resulting in stable and good fermentation performance.
비교 예 2)Comparative example 2)
상기 비교 예1의 후 발효가 완료된 배양상자를 25℃로 냉각한 후 잘게 분쇄한 느타리버섯 고체종균(원형 2호)을 4%(w/w)정도로 혼합 ·접종하였다. 22∼23℃로 유지하면서 20∼25일간 버섯균사를 활착시켰고, 바닥까지 균사활착이 완료된 후에는 15℃의 재배실로 이전하여 가습 및 환기를 시키면서 버섯발이를 유도하고 생육된 버섯을 수확하여 수량을 비교하였다.After the fermentation was completed after Comparative Example 1, the culture box was cooled to 25 ° C., and then mixed and inoculated with finely ground pulverized Pleurotus eryngii (round No. 2) at about 4% (w / w). After maintaining mycelial activity at 20 to 25 days, the mycelial activity to the bottom was completed and transferred to the 15 ℃ planting room. Compared.
실시 예 2)Example 2)
실시 예1의 발효배지를 비교 예2와 동일한 방법으로 냉각, 접종한 후 재배실로 이전하여 균사의 배양기간, 초발이 소요기간, 수확량, 오염율을 비교한 결과는표 2와 같다.The fermentation broth of Example 1 was cooled and inoculated in the same manner as in Example 2, and then transferred to the cultivation room to compare the incubation period of the hyphae, the first incubation period, the yield, and the contamination rate.
미생물제재를 첨가한 경우, 초발이에 소요되는 기간이 4일정도 단축되는 효과를 나타냈으며 4주기까지의 상자 당 전체 수확량의 20%정도 증가되었다. 또한 오염율이 2%정도로 매우 낮아 안정적인 느타리버섯 재배가 가능한 것으로 나타났다.The addition of microbial agents reduced the time required for initial shoots by about four days and increased by 20% of the total yield per box up to four cycles. In addition, the contamination rate was about 2%, showing that stable cultivation of oyster mushroom was possible.
상기와 같이 본 발명의 느타리버섯 배지발효용 미생물제재를 사용한 경우, 자연퇴적발효에 비해 오염도를 낮추어 재배 시 실패율을 낮출 수 있으며, 증수효과를 나타내 느타리버섯 재배 시 수익성제고에 매우 큰 역할을 기대할수 있고, 또한 수입에 의존하고 있는 폐솜을 국내 유휴농산부산물인 톱밥, 맥주박, 볏짚 등으로 대체할 수 있어 금후 발생될 원료조달문제를 해결할 수 있다고 판단된다.As described above, when using the microorganisms for fermentation of the oyster mushroom medium of the present invention, it is possible to lower the failure rate during cultivation by lowering the pollution degree compared to the natural sedimentation fermentation, exhibiting a increase effect can be expected to play a very large role in improving profitability when cultivating oyster mushroom In addition, waste cotton, which is dependent on imports, can be replaced by domestic idle agricultural by-products such as sawdust, beer foil, and rice straw.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20030034306A (en) * | 2001-10-22 | 2003-05-09 | 영농조합법인 착한농부들 | Methods manufacturing media for mushroom cultivation using thermophilic microorganisms |
| KR20030055106A (en) * | 2002-11-13 | 2003-07-02 | 지.티.바이오텍 주식회사 | Method of manufavturing useful microbe |
| KR20040009808A (en) * | 2002-07-23 | 2004-01-31 | 지니스생명공학 주식회사 | Cholesterol-lowering and diet mushroom and its processed |
| KR100435072B1 (en) * | 2002-08-22 | 2004-06-11 | 주식회사 한국식물병원 | medical treatment method of rotted and necrotied parts of tree using Rhodobacter sphaeroides MH-1002(KFCC-11312 |
| KR100661128B1 (en) * | 2005-04-27 | 2006-12-22 | 박미연 | Cultivation method of mushroom mycelium |
| KR100971063B1 (en) * | 2009-09-14 | 2010-07-20 | 대전광역시 | Produce and producing system for non-fermentation agaricus biscorus compost and composting method |
| WO2011030995A1 (en) * | 2009-09-14 | 2011-03-17 | Shin Byoung-Sook | Non-fermented button mushroom culture medium and a production method therefor |
| CN103387447A (en) * | 2013-07-07 | 2013-11-13 | 邬金飞 | Oyster mushroom cultivation material combination and production method thereof |
| CN115426872A (en) * | 2020-05-12 | 2022-12-02 | 株式会社乐乐 | Culture medium, fungal bed, bagged fungal bed, culture medium production method, fungal bed production method, and bagged fungal bed production method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02195822A (en) * | 1989-01-24 | 1990-08-02 | Setsuo Hattori | Cultivation of mushrooms of agaricaceae and method for increasing yield of cultured mushrooms |
| KR0153879B1 (en) * | 1995-12-27 | 1998-10-01 | 설호길 | The method of cultivating mushrooms |
| JPH1175540A (en) * | 1996-11-06 | 1999-03-23 | Asao Tekkosho:Kk | Artificial culture soil for cultivating edible mushrooms |
| KR100222090B1 (en) * | 1997-07-12 | 1999-10-01 | 설호길 | The method of cultivating mushroom |
-
2001
- 2001-03-09 KR KR1020010012372A patent/KR20010069333A/en not_active Application Discontinuation
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02195822A (en) * | 1989-01-24 | 1990-08-02 | Setsuo Hattori | Cultivation of mushrooms of agaricaceae and method for increasing yield of cultured mushrooms |
| KR0153879B1 (en) * | 1995-12-27 | 1998-10-01 | 설호길 | The method of cultivating mushrooms |
| JPH1175540A (en) * | 1996-11-06 | 1999-03-23 | Asao Tekkosho:Kk | Artificial culture soil for cultivating edible mushrooms |
| KR100222090B1 (en) * | 1997-07-12 | 1999-10-01 | 설호길 | The method of cultivating mushroom |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20030034306A (en) * | 2001-10-22 | 2003-05-09 | 영농조합법인 착한농부들 | Methods manufacturing media for mushroom cultivation using thermophilic microorganisms |
| KR20040009808A (en) * | 2002-07-23 | 2004-01-31 | 지니스생명공학 주식회사 | Cholesterol-lowering and diet mushroom and its processed |
| KR100435072B1 (en) * | 2002-08-22 | 2004-06-11 | 주식회사 한국식물병원 | medical treatment method of rotted and necrotied parts of tree using Rhodobacter sphaeroides MH-1002(KFCC-11312 |
| KR20030055106A (en) * | 2002-11-13 | 2003-07-02 | 지.티.바이오텍 주식회사 | Method of manufavturing useful microbe |
| KR100661128B1 (en) * | 2005-04-27 | 2006-12-22 | 박미연 | Cultivation method of mushroom mycelium |
| KR100971063B1 (en) * | 2009-09-14 | 2010-07-20 | 대전광역시 | Produce and producing system for non-fermentation agaricus biscorus compost and composting method |
| WO2011030995A1 (en) * | 2009-09-14 | 2011-03-17 | Shin Byoung-Sook | Non-fermented button mushroom culture medium and a production method therefor |
| CN103387447A (en) * | 2013-07-07 | 2013-11-13 | 邬金飞 | Oyster mushroom cultivation material combination and production method thereof |
| CN115426872A (en) * | 2020-05-12 | 2022-12-02 | 株式会社乐乐 | Culture medium, fungal bed, bagged fungal bed, culture medium production method, fungal bed production method, and bagged fungal bed production method |
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