CN101117622A - Yeast culture fermentation process - Google Patents
Yeast culture fermentation process Download PDFInfo
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- CN101117622A CN101117622A CNA200610150964XA CN200610150964A CN101117622A CN 101117622 A CN101117622 A CN 101117622A CN A200610150964X A CNA200610150964X A CN A200610150964XA CN 200610150964 A CN200610150964 A CN 200610150964A CN 101117622 A CN101117622 A CN 101117622A
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Abstract
The present invention relates to a fermentation process for barm culture, and can obviously reduce the loss of activity of the efficient nutrient content of the barm culture. The technical proposal is that a fermentation process of the barm culture is characterized in that the process is provided with the steps of the recovery cultivation, enlarged cultivation, ferment, and subsurface fermentation cleaving wall of bacterial strains, wherein the condition for the subsurface fermentation cleaving wall is 40 to 55 DEG C, ferment under the constant temperature is 20 to 32 hours. The biggest factor for the loss of activity of the efficient nutrient content of the barm culture is the ferment temperature and the ferment time, the ferment temperature and the ferment time chosen by the present invention can make the loss of activity of the efficient nutrient content of the barm culture lowest, and reach the cleaving wall rate over 80 percent.
Description
Technical field: the present invention relates to a kind of Yeast culture fermentation process.
Background technology: yeast culture is different from common solid state fermentation (Solid state fermentation) viable cell yeast product, it does not contain many active yeasts, be complicated leavened prod, comprise the carrier that yeast-leavened metabolite, yeast and yeast are rely and grown.Because the easy inactivation of viable cell yeast product, and in cud, also will be degraded, so the action effect instability.And yeast culture does not rely on the viable yeast bacterium because of its effect, long preservative period, and product performance are stable.
Obtaining yeast culture just needs outstanding bacterial screening is come out, and by fermentation propagation, the more important thing is to make yeast broken wall releasing product.People know that for a long time the autolysis of utilizing yeast cell disintegrates cell as the effective means (Nolf that obtains component in the cell, 1991), aqtocytolysis is owing to triggered (Chiu etc. 1997) due to the Decomposition of autolytic enzyme that cell can digest self structure under certain condition.Under proper temperature and salt concn, the breaking yeast cellule membrane of fermentation certain hour can self-dissolving.Cai Jun (2001) studies show that, the destruction that the chemistry broken wall can cause some nutritive ingredients, and add to the difficulties for the extraction of effective constituent, what have only on production technique at present that U.S. Diamond V (Da Nongwei) company claims that its yeast culture product benefit health " XP " takes is solid-state cultivation and the liquid process combined of cultivating.But the effective nutritive ingredient loss of activity of yeast culture that existing Yeast culture fermentation process obtains is all bigger.According to development of industry in China planning, the demand of China's mixed feed will reach hundred million tons of 1-2 (Sheng Yunhe, 2001) in 20 years of future.Protein fodder in short supply makes the development of China's new feed protein additive seem very urgent.And the production of the present protein fodder yeast culture of China is also irregular, does not reach required standard substantially.And U.S. Da Nongwei XP is also just impacting China feedstuff protein market.Therefore, can find out a kind of easy-regulating, can substitute the production technique of the yeast culture of Da Nongwei XP, concerning China protein fodder market, have the very meaning of important practical.
Summary of the invention: the technical problem to be solved in the present invention provides a kind of Yeast culture fermentation process that can significantly reduce the effective nutritive ingredient loss of activity of yeast culture.Prepare substratum with barley seed, stroke-physiological saline solution, agar powder, dregs of beans, wheat bran, Semen Maydis powder, distilled water.Can take the bacterial classification liquid-state fermentation technology to prepare yeast culture, select candida tropicalis and yeast saccharomyces cerevisiae for use, determine the condition of yeast culture liquid submerged fermentation.The factor of discovering the maximum that influences broken wall is a leavening temperature, secondly is salt adding amount, and what influence was minimum is fermentation time.But consider the influence to zymosan and vitamin contents, removed salt during optimization of fermentation conditions, leavening temperature that the present invention chooses and time can make the effective nutritive ingredient loss of activity of the yeast culture of acquisition minimum, reach 80% above sporoderm-broken rate.Technical scheme: a kind of Yeast culture fermentation process is characterized in that: processing step is, the recovery cultivation of bacterial strain, enlarged culturing, fermentation, submerged fermentation broken wall, and wherein the condition of submerged fermentation broken wall is 40-55 ℃ of ferment at constant temperature 20-32 hour.Beneficial effect: by the technology that the present invention proposes, the high-content of candida tropicalis and yeast saccharomyces cerevisiae polysaccharide can reach 200 μ g/ml respectively and more than the 150 μ g/ml; The rate of loss of VITAMIN is minimum, and candida tropicalis and yeast saccharomyces cerevisiae VITMAIN B1 rate of loss are not higher than 12% and 25% respectively, and the Wei ShengsuB2 rate of loss is not higher than 25% and 20% respectively, and the vitamin B6 rate of loss is not higher than 12% and 8% respectively.Adopt artificial rumen to study of the influence of different yeast cultures to sheep's hay fiber degradation rate.The result shows that the torrid zone false silk liquid culture, yeast saccharomyces cerevisiae liquid culture can improve neutral detergent fiber (NDF) degradation rate 8.02%, 6.77% (p＜0.05) respectively; Acid detergent fiber (ADF) degradation rate improves 7.73%, 9.13% (p＜0.05).In addition, add yeast culture and can improve the active and volatile fat acid yield of microorganism cellulase in the artificial rumen.Show that the yeast culture by explained hereafter of the present invention has promoter action to the cud fiber degradation, effect is close with internationally recognized beneficial health " XP ".
Embodiment:
Candida tropicalis and yeast saccharomyces cerevisiae are selected in fermentation for use.
1, the recovery of bacterial strain is cultivated
Inoculate on the inclined-plane, 30 ℃ of constant temperature culture 48 hours, 4 ℃ of preservations were transplanted once in 4-6 month.
2, enlarged culturing
Add aseptic liquid nutrient medium and shake up in cultured test tube, make bacteria suspension, the capacity of being inoculated into is in the Erlenmeyer flask that the 300ml liquid nutrient medium is housed of 1L, and places 30 ℃ of 180r/min of constant-temperature shaking culture case to cultivate 20 hours.
3, fermentation
Fermentation can solid state fermentation or liquid state fermentation.
Liquid state fermentation: above-mentioned nutrient solution is inoculated in the new aseptic liquid substratum with 20% inoculum size, 30 ℃ of liquid cultivations 72 hours of 180r/min.
Solid state fermentation: with enlarged culturing liquid with 30% inoculum size, be inoculated in the sterile grain substratum and mix, form wet compound, be tiled in the porcelain dish that is covered with three layers of sterile gauze, cover three layers of sterile gauze and lid again, constant temperature culture is 72 hours under 30 ℃ of conditions.By adjusting pH value, the acidity of change solid culture medium can significantly be optimized solid-state culture condition, and test shows, the solid-state cultivation of yeast culture is that the yeast upgrowth situation is best under 5.5 the acidity at pH.
4, the top condition of liquid state fermentation of submerged fermentation broken wall or solid state fermentation is 40-55 ℃ of ferment at constant temperature 20-32 hour, and test shows that 50 ℃ of fermentation 28h effects are best.
Claims (8)
1. Yeast culture fermentation process is characterized in that: processing step is, the recovery cultivation of bacterial strain, enlarged culturing, fermentation, submerged fermentation broken wall, and wherein the condition of submerged fermentation broken wall is 40-55 ℃ of ferment at constant temperature 20-32 hour.
2. Yeast culture fermentation process according to claim 1 is characterized in that: fermentation can be solid state fermentation or liquid state fermentation, and the condition of submerged fermentation broken wall is 50 ℃ of ferment at constant temperature 28 hours.
3. Yeast culture fermentation process according to claim 1 is characterized in that: candida tropicalis and yeast saccharomyces cerevisiae are selected in fermentation for use.
4. Yeast culture fermentation process according to claim 1 is characterized in that: the recovery culture process of bacterial strain can for, inoculate on the inclined-plane, 30 ℃ of constant temperature culture 48 hours, 4 ℃ of preservations were transplanted once in 4-6 month.
5. Yeast culture fermentation process according to claim 4, it is characterized in that: enlarged culturing technology can for, adding aseptic liquid nutrient medium in cultured test tube shakes up, make bacteria suspension, the capacity of being inoculated into is in the Erlenmeyer flask that the 300ml liquid nutrient medium is housed of 1L, and places 30 ℃ of 180r/min of constant-temperature shaking culture case to cultivate 20 hours.
6. Yeast culture fermentation process according to claim 5 is characterized in that: liquid-state fermentation technology can for, nutrient solution is inoculated in the new aseptic liquid substratum with 20% inoculum size, 30 ℃ of 180r/min are liquid to be cultivated 72 hours.
7. Yeast culture fermentation process according to claim 6, it is characterized in that: solid-state fermentation process can for, with enlarged culturing liquid with 30% inoculum size, be inoculated in the sterile grain substratum and mix, form wet compound, be tiled in the porcelain dish that is covered with three layers of sterile gauze, cover three layers of sterile gauze and lid again, constant temperature culture is 72 hours under 30 ℃ of conditions.
8. Yeast culture fermentation process according to claim 7 is characterized in that: by adjusting the pH value is under 5.5 the acidity condition, can significantly optimize solid-state culture condition.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200610150964XA CN101117622A (en) | 2006-10-31 | 2006-10-31 | Yeast culture fermentation process |
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| CNA200610150964XA CN101117622A (en) | 2006-10-31 | 2006-10-31 | Yeast culture fermentation process |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102643864A (en) * | 2012-04-27 | 2012-08-22 | 广州市富泉生物科技有限公司 | Process for preparing yeast cultures |
| CN106987530A (en) * | 2017-03-21 | 2017-07-28 | 高唐华农生物工程有限公司 | A kind of preparation method of yeast culture |
| CN110628654A (en) * | 2019-10-17 | 2019-12-31 | 嘉必优生物技术（武汉）股份有限公司 | Saccharomyces cerevisiae capable of being used for feed and preparation method and application thereof |
| CN111588656A (en) * | 2020-04-30 | 2020-08-28 | 杭州谦美化妆品有限公司 | Application of symbiotic fermentation product of hydrolyzed candida and saccharomyces cerevisiae |
| CN114680229A (en) * | 2022-03-09 | 2022-07-01 | 湖北丰甜生物科技有限公司 | Fermentation process of fishing yeast culture |
-
2006
- 2006-10-31 CN CNA200610150964XA patent/CN101117622A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102643864A (en) * | 2012-04-27 | 2012-08-22 | 广州市富泉生物科技有限公司 | Process for preparing yeast cultures |
| CN106987530A (en) * | 2017-03-21 | 2017-07-28 | 高唐华农生物工程有限公司 | A kind of preparation method of yeast culture |
| CN110628654A (en) * | 2019-10-17 | 2019-12-31 | 嘉必优生物技术（武汉）股份有限公司 | Saccharomyces cerevisiae capable of being used for feed and preparation method and application thereof |
| CN111588656A (en) * | 2020-04-30 | 2020-08-28 | 杭州谦美化妆品有限公司 | Application of symbiotic fermentation product of hydrolyzed candida and saccharomyces cerevisiae |
| CN114680229A (en) * | 2022-03-09 | 2022-07-01 | 湖北丰甜生物科技有限公司 | Fermentation process of fishing yeast culture |
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Open date: 20080206 |