CN110734346A - Fermentation process and preparation method of agricultural enzymes - Google Patents
Fermentation process and preparation method of agricultural enzymes Download PDFInfo
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- CN110734346A CN110734346A CN201910200747.4A CN201910200747A CN110734346A CN 110734346 A CN110734346 A CN 110734346A CN 201910200747 A CN201910200747 A CN 201910200747A CN 110734346 A CN110734346 A CN 110734346A
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- C05—FERTILISERS; MANUFACTURE THEREOF
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Abstract
The invention relates to a fermentation process and a preparation method of agricultural enzymes, wherein the specific formula comprises the following raw materials of agricultural animal enzymes, agricultural plant enzymes, agricultural fungus enzymes, diatomite, biochar, chitosan oligosaccharide, trehalose and carboxymethyl cellulose.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to a fermentation process and a preparation method of agricultural enzymes.
Background
The agricultural enzyme bacteria can secrete various enzymes (protease, lipase, cellulase and the like) and beneficial active substances (free amino acids, fatty acids, vitamins, nucleic acids and other active substances) required by crop production and development in life activities.
However, the agricultural enzyme products are produced by artificially adding enzymes, and the active substance can be provided by , and the using effect and the stability of the agricultural enzyme are greatly reduced.
Disclosure of Invention
The invention aims to improve steps of a fermentation production process and a preparation method of the agricultural enzyme, wherein the agricultural animal enzyme, the agricultural plant enzyme and the agricultural fungus enzyme are fermented in a normal-temperature, oxygen-introducing and independent fermentation mode, the fermented agricultural animal enzyme, the agricultural plant enzyme and the agricultural fungus enzyme are compounded and fused with diatomite, biochar and chitosan oligosaccharide in according to proportion, and the agricultural enzyme has the characteristics of nutrient diversity, balance and easiness in absorption.
In order to achieve the purpose, the technical scheme adopted by the scheme is as follows:
agricultural enzyme is prepared from agricultural animal enzyme, agricultural plant enzyme, agricultural fungus enzyme, diatomite, biochar, chitosan oligosaccharide, trehalose, and carboxymethyl cellulose.
, the agricultural enzyme comprises, by mass, 25-35 parts of agricultural animal enzyme, 25-35 parts of agricultural plant enzyme, 25-35 parts of agricultural fungus enzyme, 2-4 parts of diatomite, 2-4 parts of charcoal, 2-4 parts of trehalose, and 0.5-1 part of carboxymethyl cellulose.
, the fermentation process and the preparation method of the agricultural ferment can be processed into liquid, powder and solid particles according to requirements after being compounded according to the weight ratio.
, the preparation of the agricultural enzyme fermentation process and the agricultural enzyme preparation method comprises the following steps:
a. fermentation of the agricultural animal enzyme:
the method comprises the steps of mechanically smashing or more animal wastes including earthworm powder, termite powder, crab shells, shrimp shells, fishbone meal and the like, mixing, putting into a fermentation tank, adding white granulated sugar, biological selenium and magnetized micromolecular water into the fermentation tank, fully and uniformly mixing, and adding a composite fermentation bacterial liquid of bacillus subtilis, bacillus mucilaginosus, actinomycetes and filamentous fungi, wherein the mass parts of the animal wastes are 10-15, the mass parts of the white granulated sugar are 6-8, the mass parts of the bacterial liquid are 2-5, the mass parts of the biological selenium are 4-6 multiplied by 10-6, and the mass parts of the magnetized micromolecular water are 70-80.
The fermentation temperature is set to be 30-32 ℃, the ventilation volume is 4-6L air/min, the stirring rotation speed is 280-300 r/min, and the fermentation time is 15-20 days.
b. Fermentation of agricultural plant enzyme:
cleaning plant waste materials including kinds or more of fruits and vegetables such as potatoes, melons, grapes, garlic, onions, apples and the like, mechanically stirring the materials, mixing the materials, putting the materials into a fermentation tank, adding white granulated sugar, biological selenium and magnetized micromolecular water into the fermentation tank, fully and uniformly mixing the materials, and adding a composite fermentation bacteria solution of bacillus subtilis, bacillus amyloliquefaciens, bacillus megaterium and saccharomycetes, wherein the mass parts of the plant waste materials are 20-30, the mass parts of the white granulated sugar are 8-10, the mass parts of the bacteria solution are 2-5, the mass parts of the biological selenium are 4-6 x 10 < -6 >, and the mass parts of the magnetized micromolecular water are 60-70.
The fermentation temperature is set to be 28-30 ℃, the ventilation volume is 4-6L air/min, the stirring speed is 250-280 r/min, and the fermentation time is 10-15 days.
c. Fermentation of agricultural fungus ferment:
the method comprises the steps of cleaning or more kinds of edible fungi including shiitake mushrooms, straw mushrooms, oyster mushrooms, needle mushrooms, pleurotus eryngii and the like with water, mechanically stirring the materials, mixing the materials, putting the materials into a fermentation tank, adding white granulated sugar, biological selenium and magnetized micromolecular water into the fermentation tank, fully and uniformly mixing the materials, and adding a composite fermentation bacterial solution of bacillus subtilis, bacillus amyloliquefaciens, saccharomycetes and filamentous fungi, wherein the mass parts of fungus residues are 25-30, the mass parts of the white granulated sugar are 8-10, the mass parts of the bacterial solution are 2-5, the mass parts of the biological selenium are 4-6 x 10 < -6 >, and the mass parts of the magnetized micromolecular water are 60-70.
The fermentation temperature is set to be 28-30 ℃, the ventilation volume is 4-6L air/min, the stirring speed is 250-280 r/min, and the fermentation time is 10-15 days.
, the agricultural ferment fermentation process and the preparation method thereof comprise the following steps:
a. liquid formulation agricultural enzyme compounding:
respectively taking 25-35 parts by mass of agricultural animal enzyme, agricultural plant enzyme and agricultural fungus enzyme fermentation tank middle and upper fermentation liquor, 2-4 parts by mass of diatomite, 2-4 parts by mass of biochar, 3-5 parts by mass of chitosan oligosaccharide, 2-4 parts by mass of trehalose and 0.5-1 part by mass of carboxymethyl cellulose to effectively compound so as to prepare the liquid agricultural enzyme.
b. Compounding solid agricultural enzymes:
respectively taking 25-35 parts by mass of agricultural animal enzyme, agricultural plant enzyme and agricultural fungus enzyme fermentation tank middle and lower layer fermentation sediment, 2-4 parts by mass of diatomite, 2-4 parts by mass of biochar, 3-5 parts by mass of chitosan oligosaccharide, 2-4 parts by mass of trehalose and 0.5-1 part by mass of carboxymethyl cellulose, and effectively compounding and processing the materials into powdery or granular solid dosage form agricultural enzyme.
The invention has the beneficial effects that: the agricultural enzyme can be used as irrigation fertilizer or base fertilizer, is suitable for crops such as grain crops, economic crops, melons and fruits, vegetables, fruits and trees, and has the characteristics of improving soil microbial environment, improving disease resistance of crops, stimulating crop growth, loosening soil, keeping fertilizer, increasing crop yield and improving crop quality. Compared with the existing agricultural enzyme products, the types of the enzymes contained in the agricultural enzyme are increased, and the enzyme activity of the agricultural enzyme is improved. The product has comprehensive formula nutrients, balanced nutrition, environmental protection and no residue.
The achievement, functional features and advantages of the objects of the present invention are further illustrated in with reference to the examples.
Detailed Description
The agricultural ferment comprises the following steps:
, fermenting the agricultural ferment bacteria strain,
respectively inoculating agricultural ferment bacterium strains of bacillus subtilis, bacillus mucilaginosus, bacillus amyloliquefaciens, bacillus megaterium, actinomycetes, filamentous fungi and saccharomycetes to -grade culture media, performing activation culture to obtain -grade strains, respectively inoculating -grade strains to secondary culture media, and preparing secondary single strains.
The -grade culture medium of the bacillus subtilis, the bacillus mucilaginosus, the bacillus amyloliquefaciens and the bacillus megatherium comprises 10-20 parts by mass of cane sugar, 5-10 parts by mass of soybean meal, 1000 parts by mass of water and 15-20 parts by mass of agar, and the secondary culture medium comprises 10-20 parts by mass of cane sugar, 5-10 parts by mass of soybean meal, 0.5-1 part by mass of matrine, 0.5-1 part by mass of ascorbic acid, 0.5-1 part by mass of EDTA, and ZnCl20.5 to 1 part by mass of ZnSO40.2 to 0.5 parts by mass and 1000 parts by mass of water.
The -grade culture medium for actinomycetes and filamentous fungi comprises 1000 parts by mass of a potato juice culture medium, 30-40 parts by mass of sucrose and 15-20 parts by mass of agar, and the secondary culture medium comprises 1000 parts by mass of the potato juice culture medium, 30-40 parts by mass of sucrose, 0.5-1 part by mass of matrine, 0.5-1 part by mass of ascorbic acid, 0.5-1 part by mass of EDTA, and ZnCl20.5 to 1 part by mass of ZnSO40.2 to 0.5 parts by mass.
The -grade culture medium of the yeast comprises 1000 parts by mass of a malt wort culture medium and 15-20 parts by mass of agar, and the secondary culture medium comprises 15-20 parts by mass of bran, 0.5-1 part by mass of matrine, 0.5-1 part by mass of ascorbic acid, 0.5-1 part by mass of EDTA, and ZnCl20.5 to 1 part by mass of ZnSO40.2 to 0.5 parts by mass and 1000 parts by mass of water.
Step two: fermentation of the enzyme for agricultural animals,
selecting animal wasteThe feed additive comprises or more animal residues including earthworm powder, termite powder, crab shell, shrimp shell, fishbone powder and the like, which are mechanically stirred and mixed, the animal residues are filled into a fermentation tank, white granulated sugar, biological selenium and magnetized micromolecular water are added into the fermentation tank, the mixture is fully and uniformly mixed, and a composite fermentation bacteria solution of bacillus subtilis, bacillus mucilaginosus, actinomycetes and filamentous fungi is added, wherein the mass parts of the animal residues are 10-15, the mass parts of the white granulated sugar are 6-8, the mass parts of the bacteria solution are 2-5, and the mass parts of the biological selenium are 4-6 multiplied by 10-6The mass part of the magnetized small molecular water is 70-80. The fermentation temperature is set to be 30-32 ℃, the air flow is 4-6 Lair/min, the stirring speed is 280-300 r/min, and the fermentation time is 15-20 days.
Step three: fermentation of the enzyme of the agricultural plants,
selecting plant waste materials including kinds or more of fruits and vegetables such as potatoes, melons, grapes, garlic, onions, apples and the like, cleaning, mechanically stirring, mixing, filling into a fermentation tank, adding white granulated sugar, biological selenium and magnetized micromolecular water into the fermentation tank, fully and uniformly mixing, and adding a composite fermentation bacteria solution of bacillus subtilis, bacillus amyloliquefaciens, bacillus megaterium and saccharomycetes, wherein the mass parts of the plant waste materials are 20-30, the mass parts of the white granulated sugar are 8-10, the mass parts of the bacteria solution are 2-5, and the mass parts of the biological selenium are 4-6 multiplied by 10-6The mass part of the magnetized small molecular water is 60-70. The fermentation temperature is set to be 28-30 ℃, the ventilation volume is 4-6L air/min, the stirring speed is 250-280 r/min, and the fermentation time is 10-15 days.
Step four: fermentation of the enzyme of the agricultural fungi,
selecting any kinds or more of fungus waste materials including edible fungi such as shiitake mushrooms, straw mushrooms, oyster mushrooms, needle mushrooms, pleurotus eryngii and the like, cleaning the materials with water, mechanically stirring the materials, mixing the materials, putting the materials into a fermentation tank, adding white granulated sugar, biological selenium and magnetized small molecular water into the fermentation tank, fully and uniformly mixing the materials, and adding a composite fermentation bacteria solution of bacillus subtilis, bacillus amyloliquefaciens, saccharomycetes and filamentous fungi, wherein the mass parts of the fungus residues are 25-30, the mass parts of the white granulated sugar are 8-10, the mass parts of the bacteria solution are 2-5, and the mass parts of the biological selenium are 2-5The amount of the components is 4-6 multiplied by 10-6The mass part of the magnetized small molecular water is 60-70. The fermentation temperature is set to be 28-30 ℃, the ventilation volume is 4-6L air/min, the stirring speed is 250-280 r/min, and the fermentation time is 10-15 days.
Step five: the agricultural enzyme is compounded,
liquid formulation agricultural enzyme compounding: respectively taking 25-35 parts by mass of agricultural animal enzyme, agricultural plant enzyme and agricultural fungus enzyme fermentation tank middle and upper fermentation liquor, 2-4 parts by mass of diatomite, 2-4 parts by mass of biochar, 3-5 parts by mass of chitosan oligosaccharide, 2-4 parts by mass of trehalose and 0.5-1 part by mass of carboxymethyl cellulose to effectively compound so as to prepare the liquid agricultural enzyme.
The solid-dosage agricultural enzyme is compounded, wherein 25-35 parts by mass of agricultural animal enzyme, agricultural plant enzyme and agricultural fungus enzyme fermentation tank middle and lower layer fermentation precipitates are respectively taken to be effectively compounded with 2-4 parts by mass of diatomite, 2-4 parts by mass of biochar, 3-5 parts by mass of chitosan oligosaccharide, 2-4 parts by mass of trehalose and 0.5-1 part by mass of carboxymethyl cellulose to be processed into powdery or granular solid-dosage agricultural enzyme.
Experimental methods and data:
in order to embody the functions and effects of the agricultural enzyme, the field test is carried out on three dosage form products of the agricultural enzyme (liquid dosage form, powder dosage form and solid dosage form), and the test is as follows:
1. design of experiments
The garlic variety adopts 'Garlic Feng No. 6', the test sets 5 treatments of no bacterial fertilizer (A) applied in the bolting period, conventional bacterial fertilizer (B) applied in the bolting period, liquid agricultural ferment (C) applied in the bolting period, powdery agricultural ferment (D) applied in the bolting period and solid agricultural ferment (E) applied in the bolting period, the test sets 3 times of repetition, random block arrangement is adopted, and the planting density is 2.8 multiplied by 105Plant/ha, cell area 200m2The topdressing amount was 600 kg/ha.
2. Influence of Each treatment on the yield and quality of Garlic bulbs
TABLE 1 Effect of the treatments on the yield and quality of garlic bulbs
As can be seen from table 1, the additional application of the agricultural ferment in the bolting period of garlic can improve the yield of the bulbs, and the yield of the garlic bulbs obtained by the additional application of the agricultural ferment in a liquid dosage form, a powder dosage form and a solid dosage form is the highest, and is remarkably higher than the treatment of the additional application of conventional bacterial manure and the no additional application of bacterial manure.
The additional application of the agricultural enzyme in the bolting period of the garlic can also improve the quality of the garlic, and the content of soluble sugar, soluble protein, free amino acid, garlicin and vitamin C in the garlic bulbs of the additional application of the liquid dosage form, the powder dosage form and the solid dosage form of the agricultural enzyme are obviously higher than the treatment of the additional application of the conventional bacterial fertilizer and the non-additional application of the bacterial fertilizer.
Claims (5)
- The fermentation process of kinds of agricultural enzyme includes the following steps.
- 2. The fermentation process and the preparation method of agricultural enzymes according to claim 1, wherein the fermentation process and the preparation method of the agricultural enzymes comprise, by mass, 25-35 parts of agricultural animal enzymes, 25-35 parts of agricultural plant enzymes, 25-35 parts of agricultural fungus enzymes, 2-4 parts of diatomite, 2-4 parts of biochar, 2-4 parts of trehalose and 0.5-1 part of carboxymethyl cellulose.
- 3. The fermentation process and preparation method of agricultural enzymes according to claim 1, wherein the fermentation process and preparation method of agricultural enzymes can be formulated by weight and processed into liquid, powder and solid granules as required.
- 4. The fermentation process and preparation method of agricultural enzymes according to claim 1, wherein the preparation of the agricultural enzymes including the agricultural animal enzymes, the agricultural plant enzymes and the agricultural fungus enzymes comprises the following steps:a. fermentation of the agricultural animal enzyme:stirring any or more animal wastes including earthworm powder, termite powder, crab shells, shrimp shells, fishbone meal and the like by a machine, mixing, putting into a fermentation tank, adding white granulated sugar, biological selenium and magnetized micromolecular water into the fermentation tank, fully and uniformly mixing, and adding a composite fermentation bacterial liquid of bacillus subtilis, bacillus mucilaginosus, actinomycetes and filamentous fungi, wherein the mass part of the animal wastes is 10-15, the mass part of the white granulated sugar is 6-8, the mass part of the bacterial liquid is 2-5, the mass part of the biological selenium is 4-6 x 10 < -6 >, and the mass part of the magnetized micromolecular water is 70-80;setting the fermentation temperature to be 30-32 ℃, the ventilation volume to be 4-6L air/min, the stirring rotation speed to be 280-300 r/min, and the fermentation time to be 15-20 days;b. fermentation of agricultural plant enzyme:cleaning plant waste materials including kinds or more of fruits and vegetables such as potatoes, melons, grapes, garlic, onions, apples and the like, mechanically stirring the materials, mixing the materials, putting the materials into a fermentation tank, adding white granulated sugar, biological selenium and magnetized micromolecular water into the fermentation tank, fully and uniformly mixing the materials, and adding a composite fermentation bacteria solution of bacillus subtilis, bacillus amyloliquefaciens, bacillus megaterium and saccharomycetes, wherein the mass parts of the plant waste materials are 20-30, the mass parts of the white granulated sugar are 8-10, the mass parts of the bacteria solution are 2-5, the mass parts of the biological selenium are 4-6 multiplied by 10 < -6 >, and the mass parts of the magnetized micromolecular water are 60-70;setting the fermentation temperature to be 28-30 ℃, setting the ventilation quantity to be 4-6L air/min, setting the stirring rotation speed to be 250-280 r/min, and setting the fermentation time to be 10-15 days;c. fermentation of agricultural fungus ferment:cleaning kinds or more of edible fungi including shiitake mushrooms, straw mushrooms, oyster mushrooms, needle mushrooms, pleurotus eryngii and the like with water, mechanically stirring the materials, mixing the materials, putting the materials into a fermentation tank, adding white granulated sugar, biological selenium and magnetized micromolecular water into the fermentation tank, fully and uniformly mixing the materials, and adding a composite fermentation bacterial solution of bacillus subtilis, bacillus amyloliquefaciens, saccharomycetes and filamentous fungi, wherein the mass parts of fungus residues are 25-30, the mass parts of the white granulated sugar are 8-10, the mass parts of the bacterial solution are 2-5, the mass parts of the biological selenium are 4-6 x 10 < -6 >, and the mass parts of the magnetized micromolecular water are 60-70;the fermentation temperature is set to be 28-30 ℃, the ventilation volume is 4-6L air/min, the stirring speed is 250-280 r/min, and the fermentation time is 10-15 days.
- 5. The fermentation process and preparation method of agricultural enzymes according to claim 3, wherein the preparation method comprises the following steps:a. liquid formulation agricultural enzyme compounding:respectively taking 25-35 parts by mass of agricultural animal enzyme, agricultural plant enzyme and agricultural fungus enzyme fermentation tank middle and upper fermentation liquor, 2-4 parts by mass of diatomite, 2-4 parts by mass of biochar, 3-5 parts by mass of chitosan oligosaccharide, 2-4 parts by mass of trehalose and 0.5-1 part by mass of carboxymethyl cellulose to effectively compound to prepare liquid agricultural enzyme;b. compounding solid agricultural enzymes:respectively taking 25-35 parts by mass of agricultural animal enzyme, agricultural plant enzyme and agricultural fungus enzyme fermentation tank middle and lower layer fermentation sediment, 2-4 parts by mass of diatomite, 2-4 parts by mass of biochar, 3-5 parts by mass of chitosan oligosaccharide, 2-4 parts by mass of trehalose and 0.5-1 part by mass of carboxymethyl cellulose, and effectively compounding and processing the materials into powdery or granular solid dosage form agricultural enzyme.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111269054A (en) * | 2020-03-19 | 2020-06-12 | 广西壮族自治区林业科学研究院 | Agricultural enzyme taking agriculture and forestry processing residues as raw materials and preparation method thereof |
| CN112998018A (en) * | 2021-03-08 | 2021-06-22 | 领先生物农业股份有限公司 | Multi-effect stress-resistant agent for potatoes as well as preparation method and application of multi-effect stress-resistant agent |
| CN113136211A (en) * | 2021-03-03 | 2021-07-20 | 北京福缘地科技有限公司 | Irrigation type agricultural plant enzyme capable of reducing production cost and increasing yield in agricultural planting |
| CN114853536A (en) * | 2022-06-02 | 2022-08-05 | 广西起凤橘洲生态农业有限公司 | Preparation method and application of agricultural enzyme |
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| CN107814657A (en) * | 2017-11-09 | 2018-03-20 | 福建省恒祥渔业有限公司 | A kind of soil conditioner of oyster shell combination fish ferment and preparation method thereof |
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| CN103641528A (en) * | 2013-12-04 | 2014-03-19 | 厦门鹏原实业有限公司 | Special biological organic fertilizer for fruits and vegetables and preparation method thereof |
| CN107056546A (en) * | 2017-04-28 | 2017-08-18 | 邓和满 | Ferment fertilizer and preparation method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111269054A (en) * | 2020-03-19 | 2020-06-12 | 广西壮族自治区林业科学研究院 | Agricultural enzyme taking agriculture and forestry processing residues as raw materials and preparation method thereof |
| CN113136211A (en) * | 2021-03-03 | 2021-07-20 | 北京福缘地科技有限公司 | Irrigation type agricultural plant enzyme capable of reducing production cost and increasing yield in agricultural planting |
| CN112998018A (en) * | 2021-03-08 | 2021-06-22 | 领先生物农业股份有限公司 | Multi-effect stress-resistant agent for potatoes as well as preparation method and application of multi-effect stress-resistant agent |
| CN114853536A (en) * | 2022-06-02 | 2022-08-05 | 广西起凤橘洲生态农业有限公司 | Preparation method and application of agricultural enzyme |
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