KR20000045575A - Antioxidant cosmetic composition containing the seed extract of arctium lappa - Google Patents

Abstract

PURPOSE: A cosmetic composition containing the seed extract of Arctium lappa is provided for preventing or delaying the aging of the skin. The composition is manufactured ib the form of lotion, nourishing cream, essence cream, massage cream or cosmetic pack. CONSTITUTION: A composition contains 0.001 to 10 wt% of the seed extract of Arctium lappa having an antioxidant activity. The method for preparing the seed extract of Arctium lappa comprises the steps of: drying the seeds of Arctium lappa; powdering the dried seeds of Arctium lappa; soaking the powder in an extract solvent at room temperature for 1 to 15 days; and concentrating such extract under reduced pressure, wherein the extract solvent is selected from the group consisting of water, lower alcohol, mixed solvents of water and lower alcohol, acetone, diethylether, chloroform, hexane, and ethylacetate.

Description

Antioxidant Cosmetics Containing Allium Extract

The present invention relates to a cosmetic containing an extract separated from the fruit of the locust (Arctium lappa), and specifically relates to a safe antioxidant cosmetic because it is excellent in antioxidant activity to prevent or delay skin aging and is a natural substance.

Aging of the skin is due to oxidative damage of biomaterials by free radicals. In other words, highly reactive free radicals such as hydrogen peroxide, hydroxy radicals, and superoxide radicals, such as free radicals, peroxidize lipids that make up cell membranes, destroy cell membranes, oxidize proteins, and attack nucleic acids, causing oxidative damage. Destroy and eventually kill the cells.

Thus, preventing or delaying aging of the skin is made possible by suppressing oxidative damage of the biomaterial.

The goal of cosmetics is to maintain beautiful and healthy skin, and to delay the aging of skin that occurs naturally with age. To this end, research is being actively carried out to develop materials having excellent safety and beauty effects by searching for skin physiological activity of various natural plant components.

Materials known to have antioxidant activity so far include ascorbic acid, DL-alpha-tocopherol, beta-carotene, dibutylhydroxytoluene (BHT), and the like.

However, since these substances are used as synthetic products, they may cause skin safety problems in long-term use or in large amounts, and thus, there is an urgent need for the development of new antioxidant cosmetics that can be applied to the skin more safely and usefully.

The present inventors studied various plant extracts to search for substances that prevent skin aging. As a result, the present inventors have found that extracts obtained from allies, which are the fruits of allies, have a large antioxidant activity, thereby completing the present invention.

Allium is a plant belonging to the family Apothecary (Compositae), which grows in Korea, Japan, and China.Arctii Fructus is the fruit of the ally, and its composition is lignan, actintigenin, and lignan glycoside. arctiin) and others (T. Namba, The encyclopedia of Wakan-Yaku with Color Pictures, Vol I, pp208, Hoikusha, Tokyo, 1993). It is used for species and changyang poison.

It is an object of the present invention to provide a cosmetic containing an allergy extract, which is a natural substance that prevents or delays skin aging due to antioxidant activity.

The present invention relates to an antioxidant cosmetic containing an extract obtained from allies.

The alligator extract contained in the cosmetic of the present invention is extracted from the alligator by a conventional extraction method. In other words, after drying and grinding allies to make a fine powder, the extraction solvent is added to immerse at room temperature for 1 to 15 days to extract the active ingredient. At this time, the extraction solvent may be water, lower alcohol, mixed solvent of water and lower alcohol, acetone, diethyl ether, chloroform, hexane or ethyl acetate, 1 ~ 15ℓ of extraction solvent for 1kg of the fine powder of allies It is desirable to. The extraction solution thus extracted is concentrated under reduced pressure to form an allium extract, which is added to the cosmetic. At this time, the allium extract added to the cosmetic is 0.001 to 10% by weight, preferably 0.01 to 5% by weight relative to the cosmetic.

Antioxidant cosmetics of the present invention can be prepared in the form of conventional cosmetics such as softening (skin), astringent cosmetics, nourishing cosmetics (lotion), nutrition cream, massage cream, essence, pack.

Hereinafter, the present invention will be described in detail, but the present invention is not limited thereto.

Example 1

After washing with purified water and drying, 1kg of pulverized milk powder fine powder was placed in 4.5L of water and immersed at room temperature for 15 days, and then filtered with a filter of Whatman 2 times. The filtrate was concentrated in a vacuum condenser with a cooling capacitor to obtain a dry weight of 111 g.

Examples 2-14

Except for using the extraction solvent of Table 1 was extracted in the same manner as in Example 1 to obtain an allium extract.

Experimental Results of Examples 2-14 Extraction solvent Dry weight of extract (unit: g) Example 2 25% ethanol 108 Example 3 50% ethanol 81 Example 4 75% ethanol 81 Example 5 100% ethanol 114 Example 6 80% methanol 81 Example 7 100% methanol 93 Example 8 Acetone 105 Example 9 chloroform 84 Example 10 Hexane 93 Example 11 Normal-propanol 75 Example 12 Isopropanol 85 Example 13 Normal-butanol 116 Example 14 Ethyl acetate 63

Experimental Example 1 Antioxidant Test of Allium Extract

Antioxidant activity was determined by the thiobarbituric acid method (TBA assay) using a lipid peroxidation system by the Fenton reaction.

That is, 10 μl of ethyl linoleate, 0.2% sodium dodecyl sulfoxide (SDS), ferrous chloride 10 μ mol, hydrogen peroxide 2 μ 5 ml of 25 mM Trizma hydrochloric acid / 0.75 mM potassium chloride buffer solution containing mol was added to the dimethyl sulfoxide (DMSO) test solution of allium extract, and then incubated at 55 ° C for 16 hours, followed by 4% dibutylhydroxytoluene. 50 μl (BHT) is added to stop the reaction. 0.3 ml of this solution is added to a test tube, and 0.05 ml hydrochloric acid (3.0 ml) and 0.67% thiobarbituric acid (TBA) solution are added and boiled for 30 minutes. After cooling the reaction solution, 4.0 ml of 85% butanol was added, and the absorbance of the butanol layer was measured at 535 nm after centrifugation.

The antioxidant effect of each extract was calculated by the following formula.

The results are shown in Table 2 below.

Antioxidant Activity of Allium Extracts extract Antioxidant activity (%) Experimental concentration (㎍ / ml) ^* 10 50 100 Example 1 15 58 82 Example 2 40 62 83 Example 3 48 59 77 Example 4 47 56 73 Example 5 30 54 79 Example 6 36 53 73 Example 7 41 55 66 Example 8 31 60 83 Example 9 26 45 64 Example 10 19 52 69 Example 11 28 47 73 Example 12 28 57 83 Example 13 33 57 72 Example 14 18 49 81

* 3 times average

Allium extracts showed high activity, and were effective in both nonpolar and polar solvent extracts.

Experimental Example 2 Comparison of Antioxidant Activity between Allium Extract and Existing Antioxidants

Antioxidants of Allium Extracts of Examples 1 to 14 and Ascorbic Acid, DL-alpha-tocopherol, Beta-carotene, and Dibutylhydroxyltoluene (BHT), Existing Antioxidants The action was measured in the same manner as in Experimental Example 1 to compare the IC ₅₀ of 50% inhibitory concentration.

The results are as shown in Table 3 below.

Antioxidant Activity of Allium Extracts and Existing Antioxidants Test substance IC ₅₀ (μg / ml) Example 1 34.7 Example 2 19.1 Example 3 13.8 Example 4 15.8 Example 5 29.7 Example 6 28.2 Example 7 25.1 Example 8 25.5 Example 9 50.6 Example 10 43.0 Example 11 38.2 Example 12 28.3 Example 13 28.9 Example 14 37.8 Ascorbic acid 219.0 DL-alpha-tocopherol 33.6 Beta-carotene (β-carotene) 〉 200.0 Dibutylhydroxytoluene (BHT) 〈10

Allium extracts of Examples 1-14 showed high activity comparable to other antioxidants. In particular, these allium extracts have significant antioxidant activity despite their natural mixture.

Experimental Example 3. Inhibitory effect of allium extract on hydrogen peroxide-induced cytotoxicity

The inhibitory effect on the hydrogen peroxide-induced cytotoxicity of the allium extract prepared in Example 6 was measured using mouse-derived skin fibroblasts.

Hydrogen peroxide produces hydroxyl radicals in cells, causing lipid peroxidation, leading to cell membrane damage and cytotoxicity. Antioxidant activity of allium extract was measured as follows to test at the skin cell level.

Inoculate mouse skin fibroblasts of logarithmic growth stage into a 96 well plate at 10,000 cells per well and incubate in 80 µl of DMEM medium for 24 hours, and then dilute the media to a final concentration of 10 ^-3 M in the medium. 10 μl of hydrogen peroxide was added thereto, and at the same time, 10 μl of the dimethyl sulfoxide (DMSO) solution of the allium extract of Example 6 was added to a final concentration of 0, 50, 100, 200, and 500 μg / ml, followed by further incubation for 20 hours. 15 μl of 5 mg / ml MTT solution was added, and after 4 hours, 150 μl of dimethyl sulfoxide solution was added to the well to dissolve, and the absorbance was measured at 570 nm using a microplate reader.

The inhibitory effect on H2O peroxide-induced cytotoxicity was calculated by the following formula.

The results are as shown in Table 4 below.

Inhibitory Effect of Allium Extracts on Hydrogen Peroxide-induced Cytotoxicity Treatment Concentrations of Allium Extracts (㎍ / ml) Cytotoxic inhibitory effect ^* (%) 0 0 50 5.6 100 14.7 200 31.0 500 65.0

* 3 times average

Allium extract was found to inhibit concentration-dependently on hydrogen peroxide-induced cytotoxicity. Therefore, it can be seen that the ovarian extract inhibits cytotoxicity by inhibiting lipid peroxidation generated in mouse-derived fibroblasts.

Experimental Example 4: Cytotoxicity Test

Cytotoxicity of the alligator extract itself prepared in Example 6 was measured using the mouse-derived dermal fibroblasts as follows.

Mouse skin fibroblasts of logarithmic growth stage were inoculated into a 96 well plate at 10,000 per well and incubated for 24 hours in 80 µl of DMEM medium, and then 10 µl of the dimethyl sulfoxide solution of the alligator extract of Example 6 was added to a final concentration of 0. , 50, 100, 200, 500 ㎍ / ㎖ added to incubation for 20 hours, 15 μl of 5 mg / ㎖ MTT solution was added, and after 4 hours to dissolve by adding 150 μl of DMSO per well to the microplate reader Absorbance was measured at 570 nm.

The cytotoxicity of the alligator extract itself was expressed as cell viability, and was calculated by the following formula.

The results are as shown in Table 5 below.

Cytotoxicity of Allium Extract Treatment Concentrations of Allium Extracts (㎍ / ml) Cell viability ^* (%) 0 100.0 50 89.8 100 90.9 200 95.0 500 98.2

* 3 times average

The alligator extract of Example 6 itself did not show significant cytotoxicity compared to the negative control.

Prescription Example 1

The following is an example of the formulation of the flexible cosmetics in the cosmetics containing the allergy extract.

Here, the alliance extract refers to that of Example 6.

Prescription Example 2

Prescription examples of astringent cosmetics in cosmetics containing allium extract are as follows.

Here, the alliance extract refers to that of Example 6.

Prescription Example 3

A prescription example of nutrient cosmetic lotion (lotion) in cosmetics containing allium extract is as follows.

Here, the alliance extract refers to that of Example 6.

Prescription Example 4

A prescription example of nourishing cream in cosmetics containing allium extract is as follows.

Here, the alliance extract refers to that of Example 6.

Prescription Example 5

A prescription example of a massage cream in cosmetics containing allium extract is as follows.

Here, the alliance extract refers to that of Example 6.

Prescription Example 6

Prescription examples of essences in cosmetics containing allium extract are as follows.

Here, the alliance extract refers to that of Example 6.

Prescription Example 7

Prescription examples of packs in cosmetics containing allium extract are as follows.

Here, the alliance extract refers to that of Example 6.

ingredient Content (unit: weight%) Allium extract Glycerin Sebum 1500 Allantoin DL- Panthenol Ethanol Cyclodextrin Glycyric Acid Monoammonium Nicotinamide Sebum 40 Cured Castor Breast Preservative Pigment 3.03.02.00.10.46.00.150.30.50.3Minor Trace Amount system 100

Antioxidant cosmetics of the present invention inhibit the oxidation of biological material by free radicals to prevent or delay aging of the skin.

Claims (5)

Antioxidant cosmetic containing Arctium fructus extract. The antioxidant cosmetic composition according to claim 1, wherein the extract of allergy is contained in an amount of 0.001 to 10% by weight based on the cosmetic. The antioxidant cosmetic composition according to claim 1, wherein the alligator extract is obtained by drying and crushing the alligator to form a fine powder, and then extracting the extracted solution by immersion at room temperature for 1 to 15 days by adding an extraction solvent. The antioxidant cosmetic composition according to claim 3, wherein the extraction solvent is selected from the group consisting of water, lower alcohol, mixed solvent of water and lower alcohol, acetone, diethyl ether, chloroform, hexane and ethyl acetate. The antioxidant cosmetic composition according to claim 1, which is selected from the group consisting of softening cream, converging cream, nutrient cream, nourishing cream, massage cream, essence and pack.