KR102767028B1 - 핵산 라이브러리의 생성 방법 및 이를 실행하기 위한 조성물 및 키트 - Google Patents

핵산 라이브러리의 생성 방법 및 이를 실행하기 위한 조성물 및 키트 Download PDF

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KR102767028B1
KR102767028B1 KR1020207036874A KR20207036874A KR102767028B1 KR 102767028 B1 KR102767028 B1 KR 102767028B1 KR 1020207036874 A KR1020207036874 A KR 1020207036874A KR 20207036874 A KR20207036874 A KR 20207036874A KR 102767028 B1 KR102767028 B1 KR 102767028B1
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리차드 그린
조슈아 카프
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더 리젠츠 오브 더 유니버시티 오브 캘리포니아
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
    • C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
    • C12Q2533/107—Probe or oligonucleotide ligation

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  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
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  • Analytical Chemistry (AREA)
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  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
KR1020207036874A 2018-06-06 2019-06-05 핵산 라이브러리의 생성 방법 및 이를 실행하기 위한 조성물 및 키트 Active KR102767028B1 (ko)

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US201862681524P 2018-06-06 2018-06-06
US62/681,524 2018-06-06
PCT/US2019/035617 WO2019236726A1 (en) 2018-06-06 2019-06-05 Methods of producing nucleic acid libraries and compositions and kits for practicing same

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KR20210016560A KR20210016560A (ko) 2021-02-16
KR102767028B1 true KR102767028B1 (ko) 2025-02-13

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US (2) US11629345B2 (https=)
EP (1) EP3802864A1 (https=)
JP (2) JP7537748B2 (https=)
KR (1) KR102767028B1 (https=)
CN (1) CN112243462B (https=)
AU (1) AU2019280712B2 (https=)
CA (1) CA3100983A1 (https=)
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JP7537748B2 (ja) 2018-06-06 2024-08-21 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 核酸ライブラリを生成する方法ならびにそれを実施するための組成物およびキット
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US20230014607A1 (en) 2019-10-09 2023-01-19 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
US20240395358A1 (en) 2020-10-08 2024-11-28 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
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US20240240174A1 (en) * 2021-07-22 2024-07-18 Mgi Tech Co., Ltd. Nick-ligate stlfr
AU2022422775A1 (en) * 2021-12-24 2024-08-08 Pacbridge Partners II Investment Co. Ltd. Compositions and methods for identification of gene fusions

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JP2021526798A (ja) 2021-10-11
KR20210016560A (ko) 2021-02-16
CA3100983A1 (en) 2019-12-12
CN112243462B (zh) 2025-02-25
JP2024161413A (ja) 2024-11-19
US11629345B2 (en) 2023-04-18
AU2019280712A1 (en) 2021-01-07
EP3802864A1 (en) 2021-04-14
WO2019236726A1 (en) 2019-12-12
JP7537748B2 (ja) 2024-08-21
CN112243462A (zh) 2021-01-19
US20210222161A1 (en) 2021-07-22
AU2019280712B2 (en) 2025-11-20
US20230242907A1 (en) 2023-08-03

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