CA3100983A1 - Methods of producing nucleic acid libraries and compositions and kits for practicing same - Google Patents

Methods of producing nucleic acid libraries and compositions and kits for practicing same Download PDF

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Publication number
CA3100983A1
CA3100983A1 CA3100983A CA3100983A CA3100983A1 CA 3100983 A1 CA3100983 A1 CA 3100983A1 CA 3100983 A CA3100983 A CA 3100983A CA 3100983 A CA3100983 A CA 3100983A CA 3100983 A1 CA3100983 A1 CA 3100983A1
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Canada
Prior art keywords
ssna
ssb
bound
oligonucleotide
adapter oligonucleotide
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CA3100983A
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English (en)
French (fr)
Inventor
Richard Green
Joshua KAPP
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University of California San Diego UCSD
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University of California San Diego UCSD
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Application filed by University of California San Diego UCSD filed Critical University of California San Diego UCSD
Publication of CA3100983A1 publication Critical patent/CA3100983A1/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
    • C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
    • C12Q2533/107—Probe or oligonucleotide ligation

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA3100983A 2018-06-06 2019-06-05 Methods of producing nucleic acid libraries and compositions and kits for practicing same Pending CA3100983A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862681524P 2018-06-06 2018-06-06
US62/681,524 2018-06-06
PCT/US2019/035617 WO2019236726A1 (en) 2018-06-06 2019-06-05 Methods of producing nucleic acid libraries and compositions and kits for practicing same

Publications (1)

Publication Number Publication Date
CA3100983A1 true CA3100983A1 (en) 2019-12-12

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA3100983A Pending CA3100983A1 (en) 2018-06-06 2019-06-05 Methods of producing nucleic acid libraries and compositions and kits for practicing same

Country Status (8)

Country Link
US (2) US11629345B2 (https=)
EP (1) EP3802864A1 (https=)
JP (2) JP7537748B2 (https=)
KR (1) KR102767028B1 (https=)
CN (1) CN112243462B (https=)
AU (1) AU2019280712B2 (https=)
CA (1) CA3100983A1 (https=)
WO (1) WO2019236726A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7537748B2 (ja) 2018-06-06 2024-08-21 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 核酸ライブラリを生成する方法ならびにそれを実施するための組成物およびキット
AU2020253471A1 (en) * 2019-04-05 2021-10-14 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
US20230014607A1 (en) 2019-10-09 2023-01-19 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
US20240395358A1 (en) 2020-10-08 2024-11-28 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
GB2606158B (en) * 2021-04-26 2023-12-27 Wobble Genomics Ltd Amplification of single stranded DNA
US20240240174A1 (en) * 2021-07-22 2024-07-18 Mgi Tech Co., Ltd. Nick-ligate stlfr
AU2022422775A1 (en) * 2021-12-24 2024-08-08 Pacbridge Partners II Investment Co. Ltd. Compositions and methods for identification of gene fusions

Family Cites Families (107)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2318288A (en) 1987-08-07 1989-03-09 Genelabs Incorporated Coincidence cloning method and library
US6261774B1 (en) 1990-06-11 2001-07-17 Gilead Sciences, Inc. Truncation selex method
US5744306A (en) 1993-04-19 1998-04-28 Emory University Methods for nucleic acid detection, sequencing, and cloning using exonuclease
US5871697A (en) 1995-10-24 1999-02-16 Curagen Corporation Method and apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing
US6994958B2 (en) 1995-11-29 2006-02-07 Anthony Nolan Bone Marrow Trust Methods for separating and/or identifying DNA molecules
US5861246A (en) 1996-01-24 1999-01-19 Yale University Multiple selection process for binding sites of DNA-binding proteins
AU2324997A (en) 1996-03-15 1997-10-01 Penn State Research Foundation, The Detection of extracellular tumor-associated nucleic acid in blood plasma or ser um using nucleic acid amplification assays
CA2256700A1 (en) 1996-06-06 1997-12-11 Lynx Therapeutics, Inc. Sequencing by ligation of encoded adaptors
US6083693A (en) 1996-06-14 2000-07-04 Curagen Corporation Identification and comparison of protein-protein interactions that occur in populations
US6013438A (en) 1996-11-27 2000-01-11 Baylor College Of Medicine Assay for detecting apoptotic cells
WO1999002725A1 (en) 1997-07-11 1999-01-21 Brax Group Limited Categorising nucleic acid
US6607878B2 (en) 1997-10-06 2003-08-19 Stratagene Collections of uniquely tagged molecules
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
WO2000039333A1 (en) 1998-12-23 2000-07-06 Jones Elizabeth Louise Sequencing method using magnifying tags
CA2367400A1 (en) 1999-04-06 2000-10-12 Yale University Fixed address analysis of sequence tags
JP2003527087A (ja) 1999-08-13 2003-09-16 イェール・ユニバーシティ バイナリーコード化配列タグ
ATE317024T1 (de) 1999-12-29 2006-02-15 Keygene Nv Verfahren zur erzeugung von oligonukleotiden, im besonderen zur detektion amplifizierter restriktionsfragmente, die durch aflp erhalten wurden
US7300751B2 (en) 2000-06-30 2007-11-27 Syngenta Participations Ag Method for identification of genetic markers
US6596490B2 (en) 2000-07-14 2003-07-22 Applied Gene Technologies, Inc. Nucleic acid hairpin probes and uses thereof
US20040166499A1 (en) 2000-10-05 2004-08-26 Yoshihide Hayashizaki Oligonucleotide linkers comprising a variable cohesive portion and method for the praparation of polynucleotide libraries by using said linkers
JP4542312B2 (ja) 2001-03-09 2010-09-15 ニューゲン テクノロジーズ， インコーポレイテッド Ｒｎａ配列の増幅のための方法および組成物
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
US20040006033A1 (en) 2001-08-06 2004-01-08 Zhu York Yuan-Yuan Methods for identifying low-abundance polynucleotides and related compositions
US6927028B2 (en) 2001-08-31 2005-08-09 Chinese University Of Hong Kong Non-invasive methods for detecting non-host DNA in a host using epigenetic differences between the host and non-host DNA
US20030219878A1 (en) 2002-03-13 2003-11-27 Large Scale Biology Corporation Sticky rice
PT2348125T (pt) 2002-10-30 2017-08-29 Nuevolution As Método para a síntese de um complexo bifuncional
US8114978B2 (en) 2003-08-05 2012-02-14 Affymetrix, Inc. Methods for genotyping selected polymorphism
DE60328193D1 (de) 2003-10-16 2009-08-13 Sequenom Inc Nicht invasiver Nachweis fötaler genetischer Merkmale
US8192937B2 (en) 2004-04-07 2012-06-05 Exiqon A/S Methods for quantification of microRNAs and small interfering RNAs
EP1817430B1 (en) 2004-11-29 2009-09-16 Klinikum der Universität Regensburg Means and methods for detecting methylated dna
EP1831401B1 (en) 2004-12-29 2010-02-10 Applied Biosystems, LLC Methods, compositions, and kits for forming self-complementary polynucleotides
WO2007018601A1 (en) 2005-08-02 2007-02-15 Rubicon Genomics, Inc. Compositions and methods for processing and amplification of dna, including using multiple enzymes in a single reaction
CA2618699C (en) 2005-08-11 2012-10-02 J. Craig Venter Institute, Inc. In vitro recombination method
CA2613136C (en) 2005-08-19 2011-11-08 Bioventures, Inc. Method and substances for isolating mirnas
US20090170713A1 (en) 2005-09-29 2009-07-02 Keygene N.V. High throughput screening of mutagenized populations
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
GB0524069D0 (en) 2005-11-25 2006-01-04 Solexa Ltd Preparation of templates for solid phase amplification
EP3260556B1 (en) 2006-05-31 2019-07-31 Sequenom, Inc. Methods for the extraction of nucleic acid from a sample
AU2007260750A1 (en) 2006-06-16 2007-12-21 Sequenom, Inc. Methods and compositions for the amplification, detection and quantification of nucleic acid from a sample
US8932994B2 (en) 2006-08-24 2015-01-13 Illumina, Inc. Method for retaining even coverage of short insert libraries
WO2008045575A2 (en) 2006-10-13 2008-04-17 J. Craig Venter Institute, Inc. Sequencing method
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
WO2009032781A2 (en) 2007-08-29 2009-03-12 Sequenom, Inc. Methods and compositions for universal size-specific polymerase chain reaction
WO2009032779A2 (en) 2007-08-29 2009-03-12 Sequenom, Inc. Methods and compositions for the size-specific seperation of nucleic acid from a sample
AU2009205523A1 (en) 2008-01-14 2009-07-23 Applied Biosystems, Llc Compositions, methods, and kits for detecting ribonucleic acid
US20090203531A1 (en) 2008-02-12 2009-08-13 Nurith Kurn Method for Archiving and Clonal Expansion
DK2268834T3 (en) 2008-03-17 2015-02-02 Stichting Genetwister Ip EXPRESSION-CONNECTED GENDISCOVERY
WO2009120808A2 (en) 2008-03-26 2009-10-01 Sequenom, Inc. Restriction endonuclease enhanced polymorphic sequence detection
US9115352B2 (en) 2008-03-31 2015-08-25 Sloning Biotechnology Gmbh Method for the preparation of a nucleic acid library
WO2009133466A2 (en) 2008-04-30 2009-11-05 Population Genetics Technologies Ltd. Asymmetric adapter library construction
EP3770255A1 (en) 2008-09-16 2021-01-27 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
EP3514244B1 (en) 2009-04-03 2021-07-07 Sequenom, Inc. Nucleic acid preparation methods
US9631227B2 (en) 2009-07-06 2017-04-25 Trilink Biotechnologies, Inc. Chemically modified ligase cofactors, donors and acceptors
GB0912909D0 (en) 2009-07-23 2009-08-26 Olink Genomics Ab Probes for specific analysis of nucleic acids
WO2011143659A2 (en) 2010-05-14 2011-11-17 Fluidigm Corporation Nucleic acid isolation methods
US8828688B2 (en) 2010-05-27 2014-09-09 Affymetrix, Inc. Multiplex amplification methods
EP2580378A4 (en) 2010-06-08 2014-01-01 Nugen Technologies Inc MULTIPLEX SEQUENCING METHODS AND COMPOSITION
US8575071B2 (en) 2010-11-03 2013-11-05 Illumina, Inc. Reducing adapter dimer formation
WO2012103154A1 (en) 2011-01-24 2012-08-02 Nugen Technologies, Inc. Stem-loop composite rna-dna adaptor-primers: compositions and methods for library generation, amplification and other downstream manipulations
GB201101621D0 (en) * 2011-01-31 2011-03-16 Olink Ab Method and product
SG10201605049QA (en) 2011-05-20 2016-07-28 Fluidigm Corp Nucleic acid encoding reactions
US9139874B2 (en) 2011-07-07 2015-09-22 Life Technologies Corporation Bi-directional sequencing compositions and methods
WO2013067489A1 (en) 2011-11-05 2013-05-10 President And Fellows Of Harvard College Nucleic acid-based linkers for detecting and measuring interactions
US9506113B2 (en) 2011-12-28 2016-11-29 Ibis Biosciences, Inc. Nucleic acid ligation systems and methods
US10227587B2 (en) 2012-01-10 2019-03-12 Berry Genomics Co., Ltd. Method for constructing a plasma DNA sequencing library
US20130184165A1 (en) 2012-01-13 2013-07-18 Data2Bio Genotyping by next-generation sequencing
HK1206055A1 (en) 2012-03-02 2015-12-31 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
KR101922124B1 (ko) 2012-08-27 2018-11-26 삼성전자주식회사 시료 중 ｒｎａ로부터 ｄｎａ를 증폭하는 방법
GB2533006B (en) 2012-09-04 2017-06-07 Guardant Health Inc Systems and methods to detect copy number variation
WO2014071322A1 (en) * 2012-11-02 2014-05-08 Life Technologies Corporation Small RNA Capture, Detection and Quantification
US9816120B2 (en) 2013-01-09 2017-11-14 The Penn State Research Foundation Low sequence bias single-stranded DNA ligation
US9982255B2 (en) 2013-03-11 2018-05-29 Kailos Genetics, Inc. Capture methodologies for circulating cell free DNA
RU2694686C2 (ru) 2013-03-12 2019-07-16 Е.И.Дюпон Де Немур Энд Компани Способы идентификации вариантных сайтов распознавания для редкощепящих сконструированных средств для индукции двунитевого разрыва, и композиции с ними, и их применения
US10017807B2 (en) 2013-03-15 2018-07-10 Verinata Health, Inc. Generating cell-free DNA libraries directly from blood
CN113337604A (zh) 2013-03-15 2021-09-03 莱兰斯坦福初级大学评议会 循环核酸肿瘤标志物的鉴别和用途
US9255265B2 (en) 2013-03-15 2016-02-09 Illumina, Inc. Methods for producing stranded cDNA libraries
BR112015032512A8 (pt) 2013-06-27 2020-01-07 10X Genomics Inc método de implementação de código de barras de materiais de amostra
US9765375B2 (en) 2013-06-28 2017-09-19 General Electric Company Methods for developing binding-elements and uses thereof
RU2698125C2 (ru) 2013-08-19 2019-08-22 Эбботт Молекьюлар Инк. Библиотеки для секвенирования нового поколения
CA2929596C (en) 2013-11-13 2022-07-05 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
WO2015094861A1 (en) 2013-12-17 2015-06-25 Clontech Laboratories, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
KR102321956B1 (ko) 2014-01-31 2021-11-08 스위프트 바이오사이언시스 인코포레이티드 Dna 기질을 처리하는 개선 방법
ES2730962T3 (es) 2014-02-05 2019-11-13 Fraunhofer Ges Forschung Secuenciación libre de errores de ADN
US10208338B2 (en) 2014-03-03 2019-02-19 Swift Biosciences, Inc. Enhanced adaptor ligation
EP2921556A1 (en) * 2014-03-21 2015-09-23 Lexogen GmbH Copy number preserving RNA analysis method
CN107002122B (zh) 2014-07-25 2023-09-19 华盛顿大学 确定导致无细胞dna的产生的组织和/或细胞类型的方法以及使用其鉴定疾病或紊乱的方法
US9890375B2 (en) 2014-09-12 2018-02-13 Bgi Shenzhen Co., Limited Isolated oligonucleotide and use thereof in nucleic acid sequencing
EP3207169B1 (en) 2014-10-14 2020-07-01 MGI Tech Co., Ltd. Mate pair library construction
EP3209403A4 (en) 2014-10-24 2018-09-12 Abbott Molecular Inc. Enrichment of small nucleic acids
WO2016081798A1 (en) 2014-11-20 2016-05-26 Children's Medical Center Corporation Methods relating to the detection of recurrent and non-specific double strand breaks in the genome
JP2018504899A (ja) 2014-12-24 2018-02-22 キージーン・エン・フェー 主鎖媒介性メイトペアシーケンシング
EP3277837A1 (en) * 2015-03-31 2018-02-07 Qiagen GmbH Efficiency improving ligation methods
US12043856B2 (en) 2016-05-24 2024-07-23 The Translational Genomics Research Institute Molecular tagging methods and sequencing libraries
US11306356B2 (en) 2016-06-01 2022-04-19 Roche Sequencing Solutions, Inc. Immuno-PETE
WO2017218979A1 (en) * 2016-06-17 2017-12-21 The Broad Institute, Inc. Unbiased detection of nucleic acid modifications
EP4257701A3 (en) 2016-06-30 2023-12-20 Grail, LLC Differential tagging of rna for preparation of a cell-free dna/rna sequencing library
US10590451B2 (en) 2016-07-01 2020-03-17 Personal Genomics, Inc. Methods of constructing a circular template and detecting DNA molecules
US11299780B2 (en) 2016-07-15 2022-04-12 The Regents Of The University Of California Methods of producing nucleic acid libraries
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
CA3046617C (en) 2017-01-27 2023-02-14 Integrated Dna Technologies, Inc. Construction of next generation sequencing (ngs) libraries using competitive strand displacement
JP7062681B2 (ja) 2017-03-20 2022-05-06 イルミナ インコーポレイテッド 核酸ライブラリを調製するための方法および組成物
EP4134444B1 (en) 2017-03-23 2024-10-02 University of Washington Methods for targeted nucleic acid sequence enrichment with applications to error corrected nucleic acid sequencing
US11198865B2 (en) 2017-11-02 2021-12-14 Amanda Raine Splinted ligation adapter tagging
US11584929B2 (en) 2018-01-12 2023-02-21 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
JP7537748B2 (ja) 2018-06-06 2024-08-21 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 核酸ライブラリを生成する方法ならびにそれを実施するための組成物およびキット
AU2020253471A1 (en) 2019-04-05 2021-10-14 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid

Also Published As

Publication number Publication date
KR102767028B1 (ko) 2025-02-13
JP2021526798A (ja) 2021-10-11
KR20210016560A (ko) 2021-02-16
CN112243462B (zh) 2025-02-25
JP2024161413A (ja) 2024-11-19
US11629345B2 (en) 2023-04-18
AU2019280712A1 (en) 2021-01-07
EP3802864A1 (en) 2021-04-14
WO2019236726A1 (en) 2019-12-12
JP7537748B2 (ja) 2024-08-21
CN112243462A (zh) 2021-01-19
US20210222161A1 (en) 2021-07-22
AU2019280712B2 (en) 2025-11-20
US20230242907A1 (en) 2023-08-03

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