KR102613549B1 - 신규 세린 프로테아제 변이체 - Google Patents

신규 세린 프로테아제 변이체 Download PDF

Info

Publication number
KR102613549B1
KR102613549B1 KR1020210032885A KR20210032885A KR102613549B1 KR 102613549 B1 KR102613549 B1 KR 102613549B1 KR 1020210032885 A KR1020210032885 A KR 1020210032885A KR 20210032885 A KR20210032885 A KR 20210032885A KR 102613549 B1 KR102613549 B1 KR 102613549B1
Authority
KR
South Korea
Prior art keywords
gly
thr
ala
ser
val
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020210032885A
Other languages
English (en)
Korean (ko)
Other versions
KR20220128171A (ko
Inventor
이주희
조아라
지창준
Original Assignee
씨제이제일제당 주식회사
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to KR1020210032885A priority Critical patent/KR102613549B1/ko
Application filed by 씨제이제일제당 주식회사 filed Critical 씨제이제일제당 주식회사
Priority to CA3175414A priority patent/CA3175414A1/en
Priority to CN202280004542.9A priority patent/CN115698280A/zh
Priority to TW111108938A priority patent/TWI887529B/zh
Priority to PCT/KR2022/003415 priority patent/WO2022191653A1/ko
Priority to JP2022562328A priority patent/JP7676439B2/ja
Priority to EP22767549.3A priority patent/EP4116416A4/en
Priority to US17/997,455 priority patent/US12570967B2/en
Publication of KR20220128171A publication Critical patent/KR20220128171A/ko
Application granted granted Critical
Publication of KR102613549B1 publication Critical patent/KR102613549B1/ko
Priority to JP2024159122A priority patent/JP2025011087A/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14—Pretreatment of feeding-stuffs with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Plant Pathology (AREA)
  • Animal Husbandry (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
KR1020210032885A 2021-03-12 2021-03-12 신규 세린 프로테아제 변이체 Active KR102613549B1 (ko)

Priority Applications (9)

Application Number Priority Date Filing Date Title
KR1020210032885A KR102613549B1 (ko) 2021-03-12 2021-03-12 신규 세린 프로테아제 변이체
CN202280004542.9A CN115698280A (zh) 2021-03-12 2022-03-11 新的丝氨酸蛋白酶变体
TW111108938A TWI887529B (zh) 2021-03-12 2022-03-11 新穎絲胺酸蛋白酶變異體
PCT/KR2022/003415 WO2022191653A1 (ko) 2021-03-12 2022-03-11 신규 세린 프로테아제 변이체
CA3175414A CA3175414A1 (en) 2021-03-12 2022-03-11 Novel serine protease variant
JP2022562328A JP7676439B2 (ja) 2021-03-12 2022-03-11 新規セリンプロテアーゼ変異体
EP22767549.3A EP4116416A4 (en) 2021-03-12 2022-03-11 SERINE PROTEASE VARIANT
US17/997,455 US12570967B2 (en) 2021-03-12 2022-03-11 Serine protease variant
JP2024159122A JP2025011087A (ja) 2021-03-12 2024-09-13 新規セリンプロテアーゼ変異体

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020210032885A KR102613549B1 (ko) 2021-03-12 2021-03-12 신규 세린 프로테아제 변이체

Publications (2)

Publication Number Publication Date
KR20220128171A KR20220128171A (ko) 2022-09-20
KR102613549B1 true KR102613549B1 (ko) 2023-12-13

Family

ID=83228190

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020210032885A Active KR102613549B1 (ko) 2021-03-12 2021-03-12 신규 세린 프로테아제 변이체

Country Status (8)

Country Link
US (1) US12570967B2 (https=)
EP (1) EP4116416A4 (https=)
JP (2) JP7676439B2 (https=)
KR (1) KR102613549B1 (https=)
CN (1) CN115698280A (https=)
CA (1) CA3175414A1 (https=)
TW (1) TWI887529B (https=)
WO (1) WO2022191653A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116904428B (zh) * 2023-09-11 2024-01-02 中国农业科学院北京畜牧兽医研究所 一种改良高温碱性蛋白酶AprThc的稳定性的方法及突变体
KR20250054900A (ko) * 2023-10-16 2025-04-24 씨제이제일제당 (주) 신규 세린 프로테아제 변이체

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10619177B2 (en) 2014-12-01 2020-04-14 Novozymes A/S Method for producing a protein hydrolysate

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL114615A0 (en) * 1995-07-16 1995-11-27 Yeda Res & Dev Modulators of the function of fas receptors and other proteins
AR016969A1 (es) 1997-10-23 2001-08-01 Procter & Gamble VARIANTE DE PROTEASA, ADN, VECTOR DE EXPRESIoN, MICROORGANISMO HUESPED, COMPOSICIoN DE LIMPIEZA, ALIMENTO PARA ANIMALES Y COMPOSICIoN PARA TRATAR UN TEXTIL
US7985569B2 (en) 2003-11-19 2011-07-26 Danisco Us Inc. Cellulomonas 69B4 serine protease variants
WO2005052146A2 (en) 2003-11-19 2005-06-09 Genencor International, Inc. Serine proteases, nucleic acids encoding serine enzymes and vectors and host cells incorporating same
KR100534660B1 (ko) 2003-12-30 2005-12-08 주식회사 해찬들 단백질 분해효소 생성량이 증가된 메주의 제조방법 및 된장
KR100620092B1 (ko) 2004-12-16 2006-09-08 씨제이 주식회사 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법
CN103667323B (zh) 2007-03-12 2016-02-03 丹尼斯科美国公司 经修饰的蛋白酶
US7618801B2 (en) * 2007-10-30 2009-11-17 Danison US Inc. Streptomyces protease
CN102209778B (zh) 2008-11-11 2014-10-15 丹尼斯科美国公司 包含丝氨酸蛋白酶变体的组合物和方法
BR112014017919A2 (pt) * 2012-01-26 2017-06-27 Novozymes As uso de um poliptídeo variante, composição, polinucleotídeo isolado, constructo de ácido nucleico ou vetor de expressão, célula hospedeira de expressão recombinante, métodos de produção do pilipeptídeo, para melhoria do valor nutricional de uma ração animal, e para o tratamento de proteínas, planta, parte de planta ou célula de planta transgênica, aditivo de ração animal, ração animal, e, composição de ração animal ou detergente
AU2014214032A1 (en) * 2013-02-06 2015-07-09 Novozymes A/S Use of polypeptides having protease activity in animal feed
WO2014209789A1 (en) 2013-06-24 2014-12-31 Novozymes A/S Process of extracting oil from thin stillage
KR101632642B1 (ko) 2015-01-29 2016-06-22 씨제이제일제당 주식회사 신규한 프로모터 및 그의 용도
CN108350444A (zh) 2015-09-25 2018-07-31 诺维信公司 丝氨酸蛋白酶用于改进乙醇产量的用途
CA3028875A1 (en) 2016-07-21 2018-01-25 Novozymes A/S Serine protease variants and polynucleotides encoding same
MY186296A (en) 2016-08-31 2021-07-06 Cj Cheiljedang Corp Novel promoter and use thereof
KR20190086540A (ko) 2016-12-01 2019-07-22 바스프 에스이 조성물 중 효소의 안정화
MX2019007335A (es) 2016-12-21 2019-09-09 Dupont Nutrition Biosci Aps Métodos para usar serina proteasas termoestables.
KR102316445B1 (ko) 2019-11-29 2021-10-26 씨제이제일제당 주식회사 신규 세린 프로테아제 변이체

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10619177B2 (en) 2014-12-01 2020-04-14 Novozymes A/S Method for producing a protein hydrolysate

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NCBI, GenBank accession no. WP_067965505.1, S1 family peptidase [Nocardiopsis trehalosi] 2020.04.17

Also Published As

Publication number Publication date
CN115698280A (zh) 2023-02-03
US20230174964A1 (en) 2023-06-08
WO2022191653A1 (ko) 2022-09-15
JP2023521203A (ja) 2023-05-23
JP7676439B2 (ja) 2025-05-14
US12570967B2 (en) 2026-03-10
EP4116416A1 (en) 2023-01-11
CA3175414A1 (en) 2022-09-15
KR20220128171A (ko) 2022-09-20
JP2025011087A (ja) 2025-01-23
TWI887529B (zh) 2025-06-21
TW202237835A (zh) 2022-10-01
EP4116416A4 (en) 2023-10-11

Similar Documents

Publication Publication Date Title
AU782372B2 (en) Subtilase variants having an improved wash performance on egg stains
CN111556873A (zh) N-乙酰神经氨酸的发酵生产
KR20170035835A (ko) 푸코실레이티드 올리고당의 제조에 사용되는 알파(1,2)푸코실트랜스퍼라아제 동질 유전자형
KR102613549B1 (ko) 신규 세린 프로테아제 변이체
KR102402604B1 (ko) 신규 세린 프로테아제 변이체
AU2017384228B2 (en) Polypeptides having lysozyme activity, polynucleotides encoding same and uses and compositions thereof
CN1836035B (zh) 改进的蛋白酶及其制备方法
CN110607267B (zh) 一种绵羊李斯特菌平衡致死系统、构建方法及应用
CN102002509B (zh) 一种大肠杆菌-枯草芽孢杆菌穿梭表达载体及其应用
CN109251897A (zh) 一种猪圆环病毒3型毒株及其制备方法和应用
US20030199077A1 (en) Subtilase variants having an improved wash performance on egg stains
KR101756338B1 (ko) L-시스테인 생산용 변이미생물 및 이를 이용한 l-시스테인의 제조방법
CA2319096C (en) Method for treating acute intermittent porphyria (aip) and other porphyric diseases
CN114907997B (zh) 薯蓣皂素合成菌株构建及应用
US5700665A (en) Method for the extraction of periplasmic proteins from prokaryotic microorganisms in the presence of arginine
CN113046369B (zh) 一种新型冠状病毒的mRNA疫苗
KR102699731B1 (ko) 흰 반점 증후군 바이러스 외피 단백질을 발현하는 형질전환 미세조류를 유효성분으로 포함하는 흰 반점 증후군의 예방 또는 개선용 사료첨가제 조성물 및 이의 용도
KR102454110B1 (ko) ppGpp 생합성 관련 유전자 발현의 저해물질 탐색용 재조합 플라스미드 및 돌연변이 균주
CN114716520B (zh) 一种库德里阿兹威毕赤酵母三羧酸转运蛋白及其编码基因与应用
CN111206024B (zh) 一种表达果胶酸内切水解酶的工程菌及其应用
KR20120043657A (ko) 인간 혈액응고 7인자의 대량 생산 방법
CN111560392B (zh) miRNA表达载体及其应用
CN102653739A (zh) 利用酵母表达系统生产菊糖果糖转移酶的方法
KR20220140177A (ko) 혈관신생 관련 대사질환 치료용 약학적 조성물
JPWO2022191653A5 (https=)

Legal Events

Date Code Title Description
PA0109 Patent application

St.27 status event code: A-0-1-A10-A12-nap-PA0109

PA0201 Request for examination

St.27 status event code: A-1-2-D10-D11-exm-PA0201

R18-X000 Changes to party contact information recorded

St.27 status event code: A-3-3-R10-R18-oth-X000

R18-X000 Changes to party contact information recorded

St.27 status event code: A-3-3-R10-R18-oth-X000

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

St.27 status event code: A-1-2-D10-D22-exm-PE0701

PR0701 Registration of establishment

St.27 status event code: A-2-4-F10-F11-exm-PR0701

PR1002 Payment of registration fee

St.27 status event code: A-2-2-U10-U11-oth-PR1002

Fee payment year number: 1

PG1601 Publication of registration

St.27 status event code: A-4-4-Q10-Q13-nap-PG1601