CA3175414A1 - Novel serine protease variant - Google Patents

Novel serine protease variant

Info

Publication number
CA3175414A1
CA3175414A1 CA3175414A CA3175414A CA3175414A1 CA 3175414 A1 CA3175414 A1 CA 3175414A1 CA 3175414 A CA3175414 A CA 3175414A CA 3175414 A CA3175414 A CA 3175414A CA 3175414 A1 CA3175414 A1 CA 3175414A1
Authority
CA
Canada
Prior art keywords
amino acid
seq
serine protease
variant
present disclosure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3175414A
Other languages
English (en)
French (fr)
Inventor
Joo Hee Lee
A-Ra Cho
Chang Jun Ji
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CJ CheilJedang Corp
Original Assignee
CJ CheilJedang Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CJ CheilJedang Corp filed Critical CJ CheilJedang Corp
Publication of CA3175414A1 publication Critical patent/CA3175414A1/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14—Pretreatment of feeding-stuffs with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Animal Husbandry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
CA3175414A 2021-03-12 2022-03-11 Novel serine protease variant Pending CA3175414A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR1020210032885A KR102613549B1 (ko) 2021-03-12 2021-03-12 신규 세린 프로테아제 변이체
KR10-2021-0032885 2021-03-12
PCT/KR2022/003415 WO2022191653A1 (ko) 2021-03-12 2022-03-11 신규 세린 프로테아제 변이체

Publications (1)

Publication Number Publication Date
CA3175414A1 true CA3175414A1 (en) 2022-09-15

Family

ID=83228190

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3175414A Pending CA3175414A1 (en) 2021-03-12 2022-03-11 Novel serine protease variant

Country Status (7)

Country Link
US (1) US12570967B2 (https=)
EP (1) EP4116416A4 (https=)
JP (2) JP7676439B2 (https=)
KR (1) KR102613549B1 (https=)
CA (1) CA3175414A1 (https=)
TW (1) TWI887529B (https=)
WO (1) WO2022191653A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116904428B (zh) * 2023-09-11 2024-01-02 中国农业科学院北京畜牧兽医研究所 一种改良高温碱性蛋白酶AprThc的稳定性的方法及突变体
KR20250054900A (ko) * 2023-10-16 2025-04-24 씨제이제일제당 (주) 신규 세린 프로테아제 변이체

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR015977A1 (es) 1997-10-23 2001-05-30 Genencor Int Variantes de proteasa multiplemente substituida con carga neta alterada para su empleo en detergentes
US7985569B2 (en) 2003-11-19 2011-07-26 Danisco Us Inc. Cellulomonas 69B4 serine protease variants
US8535927B1 (en) 2003-11-19 2013-09-17 Danisco Us Inc. Micrococcineae serine protease polypeptides and compositions thereof
KR100534660B1 (ko) 2003-12-30 2005-12-08 주식회사 해찬들 단백질 분해효소 생성량이 증가된 메주의 제조방법 및 된장
KR100620092B1 (ko) 2004-12-16 2006-09-08 씨제이 주식회사 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법
EP2129779B2 (en) 2007-03-12 2018-12-26 Danisco US Inc. Modified proteases
US7618801B2 (en) 2007-10-30 2009-11-17 Danison US Inc. Streptomyces protease
US20100192985A1 (en) 2008-11-11 2010-08-05 Wolfgang Aehle Compositions and methods comprising serine protease variants
CN104350149A (zh) * 2012-01-26 2015-02-11 诺维信公司 具有蛋白酶活性的多肽在动物饲料和洗涤剂中的用途
US9441215B2 (en) * 2013-02-06 2016-09-13 Novozymes A/S Polypeptides having protease activity
US10035973B2 (en) 2013-06-24 2018-07-31 Novozymes A/S Process of extracting oil from thin stillage
CA2967538A1 (en) 2014-12-01 2016-06-09 Novozymes A/S Method for producing a protein hydrolysate
KR101632642B1 (ko) 2015-01-29 2016-06-22 씨제이제일제당 주식회사 신규한 프로모터 및 그의 용도
US10724054B2 (en) 2015-09-25 2020-07-28 Novozymes A/S Use of serine proteases for improving ethanol yield
EP3487996B1 (en) 2016-07-21 2024-04-10 Novozymes A/S Serine protease variants and polynucleotides encoding same
MY186296A (en) 2016-08-31 2021-07-06 Cj Cheiljedang Corp Novel promoter and use thereof
MX2019006425A (es) 2016-12-01 2019-08-14 Basf Se Estabilizacion de enzimas en composiciones.
DK3558026T3 (da) * 2016-12-21 2026-01-12 Int N&H Denmark Aps Fremgangsmåder til anvendelse af termostabile serinproteaser
KR102316445B1 (ko) 2019-11-29 2021-10-26 씨제이제일제당 주식회사 신규 세린 프로테아제 변이체

Also Published As

Publication number Publication date
TWI887529B (zh) 2025-06-21
CN115698280A (zh) 2023-02-03
EP4116416A1 (en) 2023-01-11
TW202237835A (zh) 2022-10-01
KR20220128171A (ko) 2022-09-20
EP4116416A4 (en) 2023-10-11
JP2023521203A (ja) 2023-05-23
KR102613549B1 (ko) 2023-12-13
WO2022191653A1 (ko) 2022-09-15
US12570967B2 (en) 2026-03-10
JP2025011087A (ja) 2025-01-23
US20230174964A1 (en) 2023-06-08
JP7676439B2 (ja) 2025-05-14

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