TWI887529B - 新穎絲胺酸蛋白酶變異體 - Google Patents

新穎絲胺酸蛋白酶變異體 Download PDF

Info

Publication number
TWI887529B
TWI887529B TW111108938A TW111108938A TWI887529B TW I887529 B TWI887529 B TW I887529B TW 111108938 A TW111108938 A TW 111108938A TW 111108938 A TW111108938 A TW 111108938A TW I887529 B TWI887529 B TW I887529B
Authority
TW
Taiwan
Prior art keywords
amino acid
seq
serine protease
variant
acid sequence
Prior art date
Application number
TW111108938A
Other languages
English (en)
Chinese (zh)
Other versions
TW202237835A (zh
Inventor
李周姬
曹亞羅
池昶俊
Original Assignee
南韓商Ｃｊ第一製糖股份有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 南韓商Ｃｊ第一製糖股份有限公司 filed Critical 南韓商Ｃｊ第一製糖股份有限公司
Publication of TW202237835A publication Critical patent/TW202237835A/zh
Application granted granted Critical
Publication of TWI887529B publication Critical patent/TWI887529B/zh

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14—Pretreatment of feeding-stuffs with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Plant Pathology (AREA)
  • Animal Husbandry (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
TW111108938A 2021-03-12 2022-03-11 新穎絲胺酸蛋白酶變異體 TWI887529B (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020210032885A KR102613549B1 (ko) 2021-03-12 2021-03-12 신규 세린 프로테아제 변이체
KR10-2021-0032885 2021-03-12

Publications (2)

Publication Number Publication Date
TW202237835A TW202237835A (zh) 2022-10-01
TWI887529B true TWI887529B (zh) 2025-06-21

Family

ID=83228190

Family Applications (1)

Application Number Title Priority Date Filing Date
TW111108938A TWI887529B (zh) 2021-03-12 2022-03-11 新穎絲胺酸蛋白酶變異體

Country Status (8)

Country Link
US (1) US12570967B2 (https=)
EP (1) EP4116416A4 (https=)
JP (2) JP7676439B2 (https=)
KR (1) KR102613549B1 (https=)
CN (1) CN115698280A (https=)
CA (1) CA3175414A1 (https=)
TW (1) TWI887529B (https=)
WO (1) WO2022191653A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116904428B (zh) * 2023-09-11 2024-01-02 中国农业科学院北京畜牧兽医研究所 一种改良高温碱性蛋白酶AprThc的稳定性的方法及突变体
KR20250054900A (ko) * 2023-10-16 2025-04-24 씨제이제일제당 (주) 신규 세린 프로테아제 변이체

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7618801B2 (en) * 2007-10-30 2009-11-17 Danison US Inc. Streptomyces protease
CN110381746A (zh) * 2016-12-21 2019-10-25 杜邦营养生物科学有限公司 使用热稳定丝氨酸蛋白酶的方法
US10619177B2 (en) * 2014-12-01 2020-04-14 Novozymes A/S Method for producing a protein hydrolysate

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL114615A0 (en) * 1995-07-16 1995-11-27 Yeda Res & Dev Modulators of the function of fas receptors and other proteins
AR016969A1 (es) 1997-10-23 2001-08-01 Procter & Gamble VARIANTE DE PROTEASA, ADN, VECTOR DE EXPRESIoN, MICROORGANISMO HUESPED, COMPOSICIoN DE LIMPIEZA, ALIMENTO PARA ANIMALES Y COMPOSICIoN PARA TRATAR UN TEXTIL
US7985569B2 (en) 2003-11-19 2011-07-26 Danisco Us Inc. Cellulomonas 69B4 serine protease variants
WO2005052146A2 (en) 2003-11-19 2005-06-09 Genencor International, Inc. Serine proteases, nucleic acids encoding serine enzymes and vectors and host cells incorporating same
KR100534660B1 (ko) 2003-12-30 2005-12-08 주식회사 해찬들 단백질 분해효소 생성량이 증가된 메주의 제조방법 및 된장
KR100620092B1 (ko) 2004-12-16 2006-09-08 씨제이 주식회사 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법
CN103667323B (zh) 2007-03-12 2016-02-03 丹尼斯科美国公司 经修饰的蛋白酶
CN102209778B (zh) 2008-11-11 2014-10-15 丹尼斯科美国公司 包含丝氨酸蛋白酶变体的组合物和方法
BR112014017919A2 (pt) * 2012-01-26 2017-06-27 Novozymes As uso de um poliptídeo variante, composição, polinucleotídeo isolado, constructo de ácido nucleico ou vetor de expressão, célula hospedeira de expressão recombinante, métodos de produção do pilipeptídeo, para melhoria do valor nutricional de uma ração animal, e para o tratamento de proteínas, planta, parte de planta ou célula de planta transgênica, aditivo de ração animal, ração animal, e, composição de ração animal ou detergente
AU2014214032A1 (en) * 2013-02-06 2015-07-09 Novozymes A/S Use of polypeptides having protease activity in animal feed
WO2014209789A1 (en) 2013-06-24 2014-12-31 Novozymes A/S Process of extracting oil from thin stillage
KR101632642B1 (ko) 2015-01-29 2016-06-22 씨제이제일제당 주식회사 신규한 프로모터 및 그의 용도
CN108350444A (zh) 2015-09-25 2018-07-31 诺维信公司 丝氨酸蛋白酶用于改进乙醇产量的用途
CA3028875A1 (en) 2016-07-21 2018-01-25 Novozymes A/S Serine protease variants and polynucleotides encoding same
MY186296A (en) 2016-08-31 2021-07-06 Cj Cheiljedang Corp Novel promoter and use thereof
KR20190086540A (ko) 2016-12-01 2019-07-22 바스프 에스이 조성물 중 효소의 안정화
KR102316445B1 (ko) 2019-11-29 2021-10-26 씨제이제일제당 주식회사 신규 세린 프로테아제 변이체

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7618801B2 (en) * 2007-10-30 2009-11-17 Danison US Inc. Streptomyces protease
US10619177B2 (en) * 2014-12-01 2020-04-14 Novozymes A/S Method for producing a protein hydrolysate
CN110381746A (zh) * 2016-12-21 2019-10-25 杜邦营养生物科学有限公司 使用热稳定丝氨酸蛋白酶的方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
網路文獻 NCBI, "S1 family peptidase [Nocardiopsis trehalosi]" 無, 2020/04/17 https://www.ncbi.nlm.nih.gov/protein/1056532615?sat=49&satkey=64064097 *

Also Published As

Publication number Publication date
CN115698280A (zh) 2023-02-03
US20230174964A1 (en) 2023-06-08
WO2022191653A1 (ko) 2022-09-15
JP2023521203A (ja) 2023-05-23
JP7676439B2 (ja) 2025-05-14
US12570967B2 (en) 2026-03-10
EP4116416A1 (en) 2023-01-11
CA3175414A1 (en) 2022-09-15
KR20220128171A (ko) 2022-09-20
KR102613549B1 (ko) 2023-12-13
JP2025011087A (ja) 2025-01-23
TW202237835A (zh) 2022-10-01
EP4116416A4 (en) 2023-10-11

Similar Documents

Publication Publication Date Title
JP2025011087A (ja) 新規セリンプロテアーゼ変異体
EP1568377A1 (en) Cysteine protease inhibitor
CN103533843A (zh) 饲料添加剂组合物
TW201234976A (en) Feed additive composition
TW201534221A (zh) 具增進發酵豆粉之產率的桿菌屬菌株及使用該菌株製造發酵豆粉的方法
JP2024096719A (ja) 新規セリンプロテアーゼ変異体
US10932481B2 (en) Thermostable protease and methods of making and using the same
EP4061935A1 (en) Thermostable phytase variants
CN101631855B (zh) 具有肌醇六磷酸酶活性且该酶活性的温度稳定性增强的多肽及编码所述多肽的核苷酸序列
JP7826219B2 (ja) 耐熱性プロテイングルタミナーゼ
JP2004504043A (ja) 複合体混合物中のガラクトース含有オリゴ糖の高温加水分解方法
CN101080491B (zh) 具有植酸酶活性的多肽和编码多肽的核苷酸序列
KR102958383B1 (ko) 신규 세린 프로테아제 변이체
JPWO2022191653A5 (https=)
TW202513795A (zh) 新穎的絲胺酸蛋白酶變體
KR20250000056A (ko) 신규 세린 프로테아제 변이체
KR20250054900A (ko) 신규 세린 프로테아제 변이체
KR102744336B1 (ko) 점액질 생성능이 저감된 미생물 및 이를 이용한 대두 발효물
KR20250004526A (ko) 아스파르트산 프로테아제 변이체
KR102885757B1 (ko) 점액질 생성능이 저감된 미생물 및 이를 이용한 대두 발효물
MX2007011479A (es) Polipeptidos y acidos nucleicos que codifican a los mismos.
KR102010836B1 (ko) 흑염소 반추위 미생물 유래의 프로테아제-kg01 유전자 및 이의 용도
CN121548638A (zh) 用于动物饲料的具有碱性磷酸酶活性的多肽