KR102613549B1 - 신규 세린 프로테아제 변이체 - Google Patents

신규 세린 프로테아제 변이체 Download PDF

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Publication number
KR102613549B1
KR102613549B1 KR1020210032885A KR20210032885A KR102613549B1 KR 102613549 B1 KR102613549 B1 KR 102613549B1 KR 1020210032885 A KR1020210032885 A KR 1020210032885A KR 20210032885 A KR20210032885 A KR 20210032885A KR 102613549 B1 KR102613549 B1 KR 102613549B1
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KR20220128171A (ko
Inventor
이주희
조아라
지창준
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씨제이제일제당 주식회사
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Priority to KR1020210032885A priority Critical patent/KR102613549B1/ko
Application filed by 씨제이제일제당 주식회사 filed Critical 씨제이제일제당 주식회사
Priority to EP22767549.3A priority patent/EP4116416A4/en
Priority to TW111108938A priority patent/TWI887529B/zh
Priority to CA3175414A priority patent/CA3175414A1/en
Priority to PCT/KR2022/003415 priority patent/WO2022191653A1/ko
Priority to US17/997,455 priority patent/US20230174964A1/en
Priority to JP2022562328A priority patent/JP7676439B2/ja
Priority to CN202280004542.9A priority patent/CN115698280A/zh
Publication of KR20220128171A publication Critical patent/KR20220128171A/ko
Application granted granted Critical
Publication of KR102613549B1 publication Critical patent/KR102613549B1/ko
Priority to JP2024159122A priority patent/JP2025011087A/ja
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14—Pretreatment of feeding-stuffs with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Physics & Mathematics (AREA)
  • Animal Husbandry (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Physiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
KR1020210032885A 2021-03-12 2021-03-12 신규 세린 프로테아제 변이체 Active KR102613549B1 (ko)

Priority Applications (9)

Application Number Priority Date Filing Date Title
KR1020210032885A KR102613549B1 (ko) 2021-03-12 2021-03-12 신규 세린 프로테아제 변이체
TW111108938A TWI887529B (zh) 2021-03-12 2022-03-11 新穎絲胺酸蛋白酶變異體
CA3175414A CA3175414A1 (en) 2021-03-12 2022-03-11 Novel serine protease variant
PCT/KR2022/003415 WO2022191653A1 (ko) 2021-03-12 2022-03-11 신규 세린 프로테아제 변이체
EP22767549.3A EP4116416A4 (en) 2021-03-12 2022-03-11 Novel serine protease variant
US17/997,455 US20230174964A1 (en) 2021-03-12 2022-03-11 Novel serine protease variant
JP2022562328A JP7676439B2 (ja) 2021-03-12 2022-03-11 新規セリンプロテアーゼ変異体
CN202280004542.9A CN115698280A (zh) 2021-03-12 2022-03-11 新的丝氨酸蛋白酶变体
JP2024159122A JP2025011087A (ja) 2021-03-12 2024-09-13 新規セリンプロテアーゼ変異体

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020210032885A KR102613549B1 (ko) 2021-03-12 2021-03-12 신규 세린 프로테아제 변이체

Publications (2)

Publication Number Publication Date
KR20220128171A KR20220128171A (ko) 2022-09-20
KR102613549B1 true KR102613549B1 (ko) 2023-12-13

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KR1020210032885A Active KR102613549B1 (ko) 2021-03-12 2021-03-12 신규 세린 프로테아제 변이체

Country Status (8)

Country Link
US (1) US20230174964A1 (enExample)
EP (1) EP4116416A4 (enExample)
JP (2) JP7676439B2 (enExample)
KR (1) KR102613549B1 (enExample)
CN (1) CN115698280A (enExample)
CA (1) CA3175414A1 (enExample)
TW (1) TWI887529B (enExample)
WO (1) WO2022191653A1 (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116904428B (zh) * 2023-09-11 2024-01-02 中国农业科学院北京畜牧兽医研究所 一种改良高温碱性蛋白酶AprThc的稳定性的方法及突变体
KR20250054900A (ko) * 2023-10-16 2025-04-24 씨제이제일제당 (주) 신규 세린 프로테아제 변이체

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10619177B2 (en) 2014-12-01 2020-04-14 Novozymes A/S Method for producing a protein hydrolysate

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL114615A0 (en) * 1995-07-16 1995-11-27 Yeda Res & Dev Modulators of the function of fas receptors and other proteins
KR100534660B1 (ko) 2003-12-30 2005-12-08 주식회사 해찬들 단백질 분해효소 생성량이 증가된 메주의 제조방법 및 된장
KR100620092B1 (ko) 2004-12-16 2006-09-08 씨제이 주식회사 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법
US7618801B2 (en) * 2007-10-30 2009-11-17 Danison US Inc. Streptomyces protease
US9801398B2 (en) * 2012-01-26 2017-10-31 Novozymes A/S Use of polypeptides having protease activity in animal feed and detergents
WO2014122161A2 (en) * 2013-02-06 2014-08-14 Novozymes A/S Polypeptides having protease activity
KR101632642B1 (ko) 2015-01-29 2016-06-22 씨제이제일제당 주식회사 신규한 프로모터 및 그의 용도
ES2907694T3 (es) 2016-08-31 2022-04-26 Cj Cheiljedang Corp Nuevo promotor y uso del mismo
EP3558026B1 (en) * 2016-12-21 2025-10-15 International N&H Denmark ApS Methods of using thermostable serine proteases
KR102316445B1 (ko) * 2019-11-29 2021-10-26 씨제이제일제당 주식회사 신규 세린 프로테아제 변이체

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10619177B2 (en) 2014-12-01 2020-04-14 Novozymes A/S Method for producing a protein hydrolysate

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NCBI, GenBank accession no. WP_067965505.1, S1 family peptidase [Nocardiopsis trehalosi] 2020.04.17

Also Published As

Publication number Publication date
US20230174964A1 (en) 2023-06-08
JP2023521203A (ja) 2023-05-23
EP4116416A4 (en) 2023-10-11
JP2025011087A (ja) 2025-01-23
KR20220128171A (ko) 2022-09-20
TW202237835A (zh) 2022-10-01
JP7676439B2 (ja) 2025-05-14
WO2022191653A1 (ko) 2022-09-15
CN115698280A (zh) 2023-02-03
TWI887529B (zh) 2025-06-21
EP4116416A1 (en) 2023-01-11
CA3175414A1 (en) 2022-09-15

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