JP7676439B2 - 新規セリンプロテアーゼ変異体 - Google Patents

新規セリンプロテアーゼ変異体 Download PDF

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JP7676439B2
JP7676439B2 JP2022562328A JP2022562328A JP7676439B2 JP 7676439 B2 JP7676439 B2 JP 7676439B2 JP 2022562328 A JP2022562328 A JP 2022562328A JP 2022562328 A JP2022562328 A JP 2022562328A JP 7676439 B2 JP7676439 B2 JP 7676439B2
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amino acid
seq
serine protease
acid sequence
present
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JP2023521203A (ja
JPWO2022191653A5 (enExample
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イ，ジュヒ
チョ，アラ
チ，チャンジュン
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シージェイ チェイルジェダン コーポレイション
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14—Pretreatment of feeding-stuffs with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Physiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
JP2022562328A 2021-03-12 2022-03-11 新規セリンプロテアーゼ変異体 Active JP7676439B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2024159122A JP2025011087A (ja) 2021-03-12 2024-09-13 新規セリンプロテアーゼ変異体

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR1020210032885A KR102613549B1 (ko) 2021-03-12 2021-03-12 신규 세린 프로테아제 변이체
KR10-2021-0032885 2021-03-12
PCT/KR2022/003415 WO2022191653A1 (ko) 2021-03-12 2022-03-11 신규 세린 프로테아제 변이체

Related Child Applications (1)

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JP2024159122A Division JP2025011087A (ja) 2021-03-12 2024-09-13 新規セリンプロテアーゼ変異体

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JP2023521203A JP2023521203A (ja) 2023-05-23
JPWO2022191653A5 JPWO2022191653A5 (enExample) 2024-04-17
JP7676439B2 true JP7676439B2 (ja) 2025-05-14

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JP2024159122A Withdrawn JP2025011087A (ja) 2021-03-12 2024-09-13 新規セリンプロテアーゼ変異体

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US (1) US20230174964A1 (enExample)
EP (1) EP4116416A4 (enExample)
JP (2) JP7676439B2 (enExample)
KR (1) KR102613549B1 (enExample)
CN (1) CN115698280A (enExample)
CA (1) CA3175414A1 (enExample)
TW (1) TWI887529B (enExample)
WO (1) WO2022191653A1 (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116904428B (zh) * 2023-09-11 2024-01-02 中国农业科学院北京畜牧兽医研究所 一种改良高温碱性蛋白酶AprThc的稳定性的方法及突变体
KR20250054900A (ko) * 2023-10-16 2025-04-24 씨제이제일제당 (주) 신규 세린 프로테아제 변이체

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150344860A1 (en) 2013-02-06 2015-12-03 Novozymes A/S Polypeptides Having Protease Activity
WO2018118815A1 (en) 2016-12-21 2018-06-28 Dupont Nutrition Biosciences Aps Methods of using thermostable serine proteases

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL114615A0 (en) * 1995-07-16 1995-11-27 Yeda Res & Dev Modulators of the function of fas receptors and other proteins
KR100534660B1 (ko) 2003-12-30 2005-12-08 주식회사 해찬들 단백질 분해효소 생성량이 증가된 메주의 제조방법 및 된장
KR100620092B1 (ko) 2004-12-16 2006-09-08 씨제이 주식회사 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법
US7618801B2 (en) * 2007-10-30 2009-11-17 Danison US Inc. Streptomyces protease
CN104350149A (zh) * 2012-01-26 2015-02-11 诺维信公司 具有蛋白酶活性的多肽在动物饲料和洗涤剂中的用途
NZ731727A (en) * 2014-12-01 2019-12-20 Novozymes As Method for producing a protein hydrolysate
KR101632642B1 (ko) 2015-01-29 2016-06-22 씨제이제일제당 주식회사 신규한 프로모터 및 그의 용도
CN110283823B (zh) 2016-08-31 2023-05-12 Cj第一制糖株式会社 新型启动子及其应用
KR102316445B1 (ko) * 2019-11-29 2021-10-26 씨제이제일제당 주식회사 신규 세린 프로테아제 변이체

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150344860A1 (en) 2013-02-06 2015-12-03 Novozymes A/S Polypeptides Having Protease Activity
WO2018118815A1 (en) 2016-12-21 2018-06-28 Dupont Nutrition Biosciences Aps Methods of using thermostable serine proteases

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
Database RefSeq [online], Accession No. WP_078763344， <https://www.ncbi.nlm.nih.gov/protein/1154855838?sat=49&satkey=64090185> 2020.04.17, [検索日2023.11.22] Definition: S1 family peptidase [Marinactinospora thermotolerans]
Database RefSeq [online],Accession No.WP_067965505 ， <https://www.ncbi.nlm.nih.gov/protein/1056532615?sat=49&satkey=64064097> 2020.04.17, [検索日2023.11.22] Definition:S1 family peptidase [Nocardiopsis trehalosi]
Database UniProtKB/TrEMBL [online], Accession No.A0A368T194，<https://rest.uniprot.org/unisave/A0A368T194?format=txt&versions=7> 2020.08.12, [検索日2023.11.22] Definition: Serineprotease {ECO:0000313|EMBL:RCV53867.1}
Database UniProtKB/TrEMBL [online], Accession No.A0A3N1ATL0，<https://rest.uniprot.org/unisave/A0A3N1ATL0?format=txt&versions=8> 2020.08.12, [検索日2023.11.22] Definition: StreptogrisinC {ECO:0000313|EMBL:ROO59928.1}
Database UniProtKB/TrEMBL [online], Accession No.A0A4P6QAJ7 ，<https://rest.uniprot.org/unisave/A0A4P6QAJ7?format=txt&versions=12> 2021.02.10, [検索日2023.11.22] Definition: Alpha-lyticprotease {ECO:0000313|EMBL:QBI56569.1}

Also Published As

Publication number Publication date
TWI887529B (zh) 2025-06-21
KR20220128171A (ko) 2022-09-20
JP2023521203A (ja) 2023-05-23
EP4116416A1 (en) 2023-01-11
US20230174964A1 (en) 2023-06-08
KR102613549B1 (ko) 2023-12-13
JP2025011087A (ja) 2025-01-23
CN115698280A (zh) 2023-02-03
TW202237835A (zh) 2022-10-01
WO2022191653A1 (ko) 2022-09-15
CA3175414A1 (en) 2022-09-15
EP4116416A4 (en) 2023-10-11

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