CA3175414A1 - Novel serine protease variant - Google Patents

Novel serine protease variant

Info

Publication number
CA3175414A1
CA3175414A1 CA3175414A CA3175414A CA3175414A1 CA 3175414 A1 CA3175414 A1 CA 3175414A1 CA 3175414 A CA3175414 A CA 3175414A CA 3175414 A CA3175414 A CA 3175414A CA 3175414 A1 CA3175414 A1 CA 3175414A1
Authority
CA
Canada
Prior art keywords
amino acid
seq
serine protease
variant
present disclosure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3175414A
Other languages
English (en)
French (fr)
Inventor
Joo Hee Lee
A-Ra Cho
Chang Jun Ji
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CJ CheilJedang Corp
Original Assignee
CJ CheilJedang Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CJ CheilJedang Corp filed Critical CJ CheilJedang Corp
Publication of CA3175414A1 publication Critical patent/CA3175414A1/en
Pending legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/189Enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14Pretreatment of feeding-stuffs with enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21Serine endopeptidases (3.4.21)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/02Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/04Libraries containing only organic compounds
    • C40B40/06Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Physics & Mathematics (AREA)
  • Animal Husbandry (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Physiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
CA3175414A 2021-03-12 2022-03-11 Novel serine protease variant Pending CA3175414A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR1020210032885A KR102613549B1 (ko) 2021-03-12 2021-03-12 신규 세린 프로테아제 변이체
KR10-2021-0032885 2021-03-12
PCT/KR2022/003415 WO2022191653A1 (ko) 2021-03-12 2022-03-11 신규 세린 프로테아제 변이체

Publications (1)

Publication Number Publication Date
CA3175414A1 true CA3175414A1 (en) 2022-09-15

Family

ID=83228190

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3175414A Pending CA3175414A1 (en) 2021-03-12 2022-03-11 Novel serine protease variant

Country Status (8)

Country Link
US (1) US20230174964A1 (enExample)
EP (1) EP4116416A4 (enExample)
JP (2) JP7676439B2 (enExample)
KR (1) KR102613549B1 (enExample)
CN (1) CN115698280A (enExample)
CA (1) CA3175414A1 (enExample)
TW (1) TWI887529B (enExample)
WO (1) WO2022191653A1 (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116904428B (zh) * 2023-09-11 2024-01-02 中国农业科学院北京畜牧兽医研究所 一种改良高温碱性蛋白酶AprThc的稳定性的方法及突变体
KR20250054900A (ko) * 2023-10-16 2025-04-24 씨제이제일제당 (주) 신규 세린 프로테아제 변이체

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL114615A0 (en) * 1995-07-16 1995-11-27 Yeda Res & Dev Modulators of the function of fas receptors and other proteins
KR100534660B1 (ko) 2003-12-30 2005-12-08 주식회사 해찬들 단백질 분해효소 생성량이 증가된 메주의 제조방법 및 된장
KR100620092B1 (ko) 2004-12-16 2006-09-08 씨제이 주식회사 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법
US7618801B2 (en) * 2007-10-30 2009-11-17 Danison US Inc. Streptomyces protease
US9801398B2 (en) * 2012-01-26 2017-10-31 Novozymes A/S Use of polypeptides having protease activity in animal feed and detergents
WO2014122161A2 (en) * 2013-02-06 2014-08-14 Novozymes A/S Polypeptides having protease activity
EP3227456A1 (en) * 2014-12-01 2017-10-11 Novozymes A/S Method for producing a protein hydrolysate
KR101632642B1 (ko) 2015-01-29 2016-06-22 씨제이제일제당 주식회사 신규한 프로모터 및 그의 용도
ES2907694T3 (es) 2016-08-31 2022-04-26 Cj Cheiljedang Corp Nuevo promotor y uso del mismo
EP3558026B1 (en) * 2016-12-21 2025-10-15 International N&H Denmark ApS Methods of using thermostable serine proteases
KR102316445B1 (ko) * 2019-11-29 2021-10-26 씨제이제일제당 주식회사 신규 세린 프로테아제 변이체

Also Published As

Publication number Publication date
US20230174964A1 (en) 2023-06-08
JP2023521203A (ja) 2023-05-23
EP4116416A4 (en) 2023-10-11
JP2025011087A (ja) 2025-01-23
KR20220128171A (ko) 2022-09-20
TW202237835A (zh) 2022-10-01
JP7676439B2 (ja) 2025-05-14
WO2022191653A1 (ko) 2022-09-15
CN115698280A (zh) 2023-02-03
TWI887529B (zh) 2025-06-21
EP4116416A1 (en) 2023-01-11
KR102613549B1 (ko) 2023-12-13

Similar Documents

Publication Publication Date Title
JP2025011087A (ja) 新規セリンプロテアーゼ変異体
AU2015341922B2 (en) Polypeptides having serine protease activity and polynucleotides encoding same and their application in animal feed
CA2481489A1 (en) Cysteine protease inhibitor
JP2024096719A (ja) 新規セリンプロテアーゼ変異体
JP6086614B2 (ja) 生理活性分子の融合
US10932481B2 (en) Thermostable protease and methods of making and using the same
CN101631855B (zh) 具有肌醇六磷酸酶活性且该酶活性的温度稳定性增强的多肽及编码所述多肽的核苷酸序列
EP4061935A1 (en) Thermostable phytase variants
CN101080491B (zh) 具有植酸酶活性的多肽和编码多肽的核苷酸序列
CN117529554A (zh) 包含转谷氨酰胺酶的酶剂及其用途
CN110621777A (zh) 胰蛋白酶样丝氨酸蛋白酶及其用途
JPWO2022191653A5 (enExample)
KR20250000056A (ko) 신규 세린 프로테아제 변이체
TW202513795A (zh) 新穎的絲胺酸蛋白酶變體
KR20250054900A (ko) 신규 세린 프로테아제 변이체
KR102744336B1 (ko) 점액질 생성능이 저감된 미생물 및 이를 이용한 대두 발효물
KR102885757B1 (ko) 점액질 생성능이 저감된 미생물 및 이를 이용한 대두 발효물
US20060127982A1 (en) Development of an asporogenic bacillus licheniformis and production of keratinase therefrom
MX2007011479A (es) Polipeptidos y acidos nucleicos que codifican a los mismos.
KR102010836B1 (ko) 흑염소 반추위 미생물 유래의 프로테아제-kg01 유전자 및 이의 용도

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20221012

EEER Examination request

Effective date: 20221012

EEER Examination request

Effective date: 20221012

EEER Examination request

Effective date: 20221012

EEER Examination request

Effective date: 20221012

EEER Examination request

Effective date: 20221012