KR102593843B1 - Oral composition for protection, improvement and treatment against UV-induced skin damages and method for protecting skin - Google Patents

Abstract

The present invention relates to a composition for preventing, improving or treating skin damage caused by ultraviolet rays and a method for protecting skin using the same, comprising as an active ingredient an extraction mixture containing green tea extract, bisuri extract and grape seed extract, and a composition for preventing, improving or treating skin damage caused by ultraviolet rays. Compositions for preventing, improving or treating damage; This relates to a method of protecting the skin using this.

Description

Composition for preventing, improving or treating skin damage caused by ultraviolet rays and method for protecting skin using the same {Oral composition for protection, improvement and treatment against UV-induced skin damages and method for protecting skin}

The present invention relates to a composition for preventing, improving or treating skin damage caused by ultraviolet rays and a skin protection method using the same. More specifically, it relates to a composition for preventing, improving or treating various symptoms and diseases that can be caused by ultraviolet rays. It relates to a composition that can protect the skin and a method of protecting the skin using the same.

The skin plays a role in protecting our body from external harmful factors. Ultraviolet rays (ultraviolet, UV) are a hazard transmitted directly to the skin and are classified into UVA (320-400 nm), UVB (290-320 nm), and UVC (200-290 nm) depending on the length of the wavelength. UVA and UVB directly damage DNA or generate reactive oxygen species, accelerating skin aging such as loss of skin elasticity and wrinkle formation. In particular, keratinocytes present in the skin epidermis express the aquaporin 3 protein, which transports moisture into cells when exposed to ultraviolet rays, and HAS2 (hyaluronan synthase 2), an enzyme that produces hyaluronic acid (HA). This decrease causes skin dryness.

Ultraviolet rays are an environmental factor that requires attention because they can not only cause skin aging but also cause skin diseases or worsen existing skin diseases. Even as the intensity of ultraviolet rays gets stronger year by year, skin problems and health expenditures due to them are also increasing every year. Representative skin diseases caused by ultraviolet rays include skin cancer such as squamous cell cancer and basal cell cancer. Although topical sunscreen is commonly used, it is realistically very difficult to apply sunscreen to all parts of the body exposed to ultraviolet rays every 2 to 3 hours.

Because of this, many studies have been conducted to protect against ultraviolet rays by eating them. For example, European Patent Application Publication EP0712630 A2 (JBC Cosmetics) describes an orally administered formulation containing carotenoids, tocopherol, ascorbic acid and selenium. This preparation is intended to prevent browning of the skin and sun allergy (photodermatosis). α-Carotene, β-carotene and lycopene are used as carotenoids in a daily dose of 60 to 150 mg. Thus, the prior art literature describes many 'oral' formulations that are supposed to increase the skin's own sun protection factors, many of which are based on α- or β-carotene, but supplementation with β-carotene is not recommended. Since research has shown that it may increase the risk of lung cancer, there is a need for a replacement or partial replacement for β-carotene in existing oral sun protection formulations.

Therefore, if an edible sunscreen supplement that is based on natural products and has no side effects is developed, it is expected to be widely applied.

One aspect of the present invention is to provide a composition for preventing, improving or treating skin damage caused by ultraviolet rays.

Another aspect of the present invention is to provide a method for protecting skin from ultraviolet rays.

According to one aspect of the present invention, there is provided a composition for preventing, improving or treating skin damage caused by ultraviolet rays, comprising as an active ingredient an extraction mixture containing green tea extract, bisuri extract and grape seed extract.

According to another aspect of the present invention, there is provided a method of protecting skin from ultraviolet rays, comprising the step of orally administering the composition of the present invention before, during, and after ultraviolet ray exposure.

The composition for preventing, improving or treating skin damage caused by ultraviolet rays of the present invention can be applied by oral and parenteral routes to not only prevent and improve skin damage caused by ultraviolet rays, but also obtain a treatment effect for diseases caused or worsened by ultraviolet rays. there is. Therefore, ultimately, various skin problems caused by ultraviolet rays can be resolved.

Figure 1 shows the results of a DPPH radical scavenging activity experiment.
Figure 2 shows the results of the ABTS radical scavenging activity experiment.
Figure 3 shows the evaluation results of IGA (Investigator's global assessment).
Figure 4 shows the results of TEWL (Transepidermal water loss) measurement.
Figure 5 shows the EI (Erythema index) measurement results.
Figure 6 shows the results of MI (Melasma index) measurement.
Figure 7 shows the skin hydration measurement results.
Figure 8 shows a photograph measuring the minimal erythema dose, and the related photograph is shown in Figure 9.

Hereinafter, preferred embodiments of the present invention will be described with reference to the attached drawings. However, the embodiments of the present invention may be modified into various other forms, and the scope of the present invention is not limited to the embodiments described below.

According to the present invention, a composition that can ultimately reduce various skin problems caused by ultraviolet rays and a method of protecting the skin from ultraviolet rays using the same are provided.

According to the present invention, a composition for preventing, improving or treating skin damage caused by ultraviolet rays is provided, and the composition contains an extraction mixture containing green tea extract, Visuri extract and grape seed extract as an active ingredient.

At this time, the green tea extract is obtained by extracting green tea leaves using water as an extraction solvent, and can be used in powder form after drying. For example, the extraction may be carried out at room temperature or may be hot water extraction, and if necessary, a concentration step may be performed and the filtered result may be used. Furthermore, the extract may be dried and used in the form of a dry powder.

The bisuri extract is obtained by drying at least some of the whole plant parts of bisurigi, such as stems and leaves, and then using water as a solvent at 100 to 150°C, more specifically 110 to 130°C, for example, 121°C. In this case, the extraction time may be 1 hour to 12 hours, for example, 2 hours to 8 hours, or 4 hours to 6 hours, and if necessary, after performing the concentration step, the filtered result is It can be used, and furthermore, the extract can be dried and used in powder form after drying.

Grape seed extract is obtained by grinding grape seeds and using water, alcohol, or an aqueous alcohol solution as an extraction solvent. The alcohol may be, for example, ethanol. For example, an aqueous ethanol solution may be used as an extraction solvent. Furthermore, if necessary, the filtered result can be used after performing a concentration step. Furthermore, the extract can be dried and used in powder form after drying.

In the composition of the present invention, the effect of preventing, improving or treating skin damage caused by ultraviolet rays is based on the active ingredients, and the extraction mixture contains 20 to 60% by weight of green tea extract and 10 to 30% by weight of bisuri extract based on the total weight of the extraction mixture. % and 20 to 60% by weight of grape seed extract, for example, 15 to 25% by weight of bisuri extract; and 75 to 85% by weight of a mixture of green tea extract and grape seed extract.

Meanwhile, the extraction mixture may include 1 to 3 parts by weight of green tea extract and 1 to 3 parts by weight of grape seed extract per 1 part by weight of the bisuri extract, for example, green tea extract, bisuri extract, and grape seed extract. and grape seed extract in a weight ratio of 1 to 3 parts by weight: 1 part by weight: 1 to 3 parts by weight, for example, 2:1:1, 3:1:1, or 1:1:3.

If the mixing ratio of the extraction mixture is outside the scope of the present invention, the antioxidant effect may be insufficient, and therefore the effect of preventing, improving, or treating skin damage caused by ultraviolet rays, which is the goal of the present invention, may be reduced.

Furthermore, the composition of the present invention may contain at least one additional ingredient selected from the group consisting of nicotinic acid amide, beta carotene, vitamins, and selenium, and the vitamin is selected from the group consisting of vitamin C, vitamin D, and vitamin E. It may contain at least one vitamin ingredient. For example, the composition of the present invention may further include nicotinic acid amide, beta carotene, vitamin D, and selenium, and may further include vitamins C and E.

The composition of the present invention, for example, based on 2000 mg of the total composition, contains 150 to 250 mg of green tea extract, 50 to 150 mg of Visuri extract, 50 to 150 mg of grape seed extract, 3 to 20 mg of nicotinic acid amide, and 0.1 to 10 mg of beta carotene. , 50 to 1500 IU of vitamin D and 15 to 60 cmg of selenium, 50 to 150 mg of vitamin C and 2 to 15 mg of vitamin E, along with excipients and/or additives. However, in the present invention, beta-carotene is not included in an amount exceeding 10 mg to prevent side effects.

The composition of the present invention may be a composition for oral administration for preventing or improving skin damage. More specifically, the composition for oral administration may be a pharmaceutical composition for oral administration, a health functional food composition, or a health supplement composition. It may be.

At this time, pharmaceutical compositions for oral administration for preventing, improving or treating skin damage caused by ultraviolet rays are used for preventing skin damage caused by ultraviolet rays, improving skin damage caused by ultraviolet rays, protecting skin from ultraviolet rays, or causing or worsening skin damage caused by ultraviolet rays. It may be for treating skin diseases.

The skin disease may be at least one skin disease selected from the group consisting of sunlight allergy, photosensitivity dermatitis, skin cancer, lupus, melasma, atopic dermatitis, folliculitis, eczema, and dyspigmentation.

A pharmaceutical composition for oral administration for preventing, improving or treating skin damage caused by ultraviolet rays is formulated with at least one solid preparation selected from the group consisting of tablets, pills, powders, granules, and capsules. , may be a composition for improvement or treatment.

The pharmaceutical composition of the present invention is prepared by a conventional method according to the purpose of use, at least one solid preparation selected from the group consisting of tablets, pills, powders, granules, and capsules, or as a suspension, emulsion, syrup, aerosol, etc. It can be administered through the oral route in an oral dosage form of at least one liquid preparation selected from the group consisting of, etc.

Examples of suitable carriers, excipients and diluents that may be included in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginates, gelatin, calcium phosphate, calcium silicate, cellulose, Examples include methyl cellulose, amorphous cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil.

In addition, the pharmaceutical composition may further include fillers, anti-coagulants, lubricants, wetting agents, flavorings, emulsifiers, preservatives, etc.

Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and these solid preparations include at least one excipient in the pharmaceutical composition, such as starch, calcium carbonate, sucrose, lactose, gelatin, etc. It can be formulated by mixing. Additionally, in addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. In the present invention, the solid preparation may contain 10 to 40% by weight of the active ingredient based on the total weight of the solid preparation.

Oral liquid preparations include suspensions, oral solutions, emulsions, and syrups. In addition to the commonly used simple diluents such as water and liquid paraffin, various excipients may be included, such as wetting agents, sweeteners, fragrances, and preservatives.

The pharmaceutical composition of the present invention is administered or applied in a pharmaceutically effective amount to a subject in need of preventing, improving, or treating skin damage caused by ultraviolet rays.

The composition of the present invention may be administered as an individual therapeutic agent or in combination with other therapeutic agents, may be administered sequentially or simultaneously with conventional therapeutic agents, and may be administered singly or multiple times. Considering all of the above factors, it is important to administer an amount that can achieve maximum effect with the minimum amount without side effects, and this can be easily determined by a person skilled in the art. However, since it may increase or decrease depending on the route of administration, severity of disease, gender, weight, age, etc., the above dosage does not limit the scope of the present invention in any way.

Furthermore, according to the present invention, a food containing as an active ingredient an extraction mixture containing green tea extract, bisuri extract, and grape seed extract is provided. The food of the present invention is a food composition and includes all health functional foods, health supplements, etc., and the form of the food composition is not particularly limited. Any food in any form such as liquid, solid, or other fluids is included. It is intended to be.

For example, when the food composition of the present invention is a food composition such as a functional beverage, the food composition may contain sweeteners, flavoring agents, physiologically active ingredients, minerals, etc. in addition to the active ingredients.

Sweeteners can be used in amounts to give foods an appropriate sweet taste and can be natural or synthetic. Preferably, a natural sweetener is used, and natural sweeteners include sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose, and maltose.

Flavoring agents can be used to improve taste or aroma, and both natural and synthetic ones can be used. Preferably, natural products are used. When using natural products, they can serve the purpose of enhancing nutrition in addition to flavor. Natural flavoring agents may be obtained from apples, lemons, tangerines, grapes, strawberries, peaches, etc., or may be obtained from green tea leaves, coriander leaves, bamboo leaves, cinnamon, chrysanthemum leaves, jasmine, etc. You can also use things obtained from ginseng (red ginseng), bamboo shoots, aloe vera, and ginkgo nuts. Natural flavoring agents may be liquid concentrates or solid extracts. In some cases, synthetic flavoring agents may be used, and the synthetic flavoring agents may include esters, alcohols, aldehydes, terpenes, etc.

As physiologically active substances, catechins such as catechin, epicatechin, gallocatechin, and epigallocatechin, and vitamins such as retinol, ascorbic acid, tocopherol, calciferol, thiamine, and riboflavin can be used. As minerals, calcium, magnesium, chromium, cobalt, copper, fluoride, germanium, iodine, iron, lithium, magnesium, manganese, molybdenum, phosphorus, potassium, selenium, silicon, sodium, sulfur, vanadium, zinc, etc. can be used.

In addition, the food composition of the present invention may contain preservatives, emulsifiers, acidulants, thickeners, etc., as necessary, in addition to the above-mentioned sweeteners. These preservatives, emulsifiers, etc. are preferably added in very small amounts as long as they can achieve the purpose for which they are added. When expressed numerically, trace amount means a range of 0.0005% by weight to about 0.5% by weight based on the total weight of the food composition.

Preservatives that can be used include calcium sodium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate, and EDTA (ethylenediaminetetraacetic acid). Emulsifiers that can be used include acacia gum, carboxymethylcellulose, xanthan gum, pectin, etc. Acidulants that can be used include acidic acid, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, and phosphoric acid. These acidulants may be added to ensure that the food composition has an appropriate acidity for the purpose of suppressing the growth of microorganisms in addition to improving taste. Thickening agents that can be used include suspending agents, settling agents, gel forming agents, bulking agents, etc.

Meanwhile, the composition may be a composition for external use for preventing, improving or treating skin damage caused by ultraviolet rays. More specifically, the composition for external use may be a cosmetic composition for preventing or improving skin damage caused by ultraviolet rays or skin damage caused by ultraviolet rays. It may be a pharmaceutical composition for prevention, improvement or treatment.

Pharmaceutical composition preparations for parenteral administration include sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried preparations, and suppositories. Non-aqueous solvents and suspensions may include propylene glycol, polyethylene glycol, and vegetable oil such as olive oil.

When the composition of the present invention is a cosmetic composition, the cosmetic composition can be prepared in various forms, such as emulsions, lotions, creams (oil-in-water type, water-in-oil type, multiphase), solutions, suspensions (anhydrous and aqueous), It can be prepared in the form of anhydrous products (oil and glycol-based), gels, masks, packs or powders.

In addition to the active ingredients, the composition of the present invention may contain additional ingredients, including carriers acceptable in cosmetic preparations and/or other additives usually blended into cosmetics as needed.

Here, “acceptable carrier in cosmetic preparations” refers to a compound or composition that is already known and used or a compound or composition to be developed in the future that can be included in cosmetic preparations and has no toxicity beyond that to which the human body can adapt upon contact with the skin. The carrier may be included in the composition of the present invention in an amount of about 1% by weight to about 99.99% by weight based on the total weight of the composition, preferably about 50% by weight to about 99% by weight of the total weight of the composition.

However, since the above ratio varies depending on the above-mentioned formulation of the cosmetic, as well as the specific application area and preferred application amount, the above ratio should not be understood as limiting the scope of the present invention in any respect.

Meanwhile, the additional ingredients include alcohol, oil, surfactant, fatty acid, silicone oil, wetting agent, moisturizer, viscosity modifier, emulsion, stabilizer, sunscreen, coloring agent, fragrance, antioxidant, plant extract, pH adjuster, colorant, etc. It can be exemplified. The compounds or compositions that can be used as alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, moisturizers, viscosity modifiers, emulsions, stabilizers, sunscreens, coloring agents, and fragrances are already known in the art, so those skilled in the art will An appropriate material or composition may be selected and used.

Furthermore, according to the present invention, a method of protecting the skin from ultraviolet rays is provided. The method of protecting the skin from ultraviolet rays of the present invention includes applying the composition of the present invention described above before, during, and after ultraviolet ray exposure. It includes the step of oral administration. For example, it can be administered orally within 12 hours before exposure to ultraviolet light, such as 6 hours, 3 hours, 1 hour and 30 minutes, during exposure to ultraviolet light, and/or within 12 hours after exposure to ultraviolet light, such as 6 hours. , can be administered orally within 3 hours, 1 hour, and 30 minutes.

The oral administration may be oral administration of the composition once to three times a day, but is not limited thereto.

The composition of the present invention can be especially formulated as an oral formulation, and therefore can be usefully used by patients suffering from or at risk of suffering from various skin diseases caused or worsened by ultraviolet rays, and furthermore, by general modern people who are aiming for skin health. It can also be widely applied to people to maintain and prevent skin health caused by ultraviolet rays. In this case, it can provide a skin protection effect against ultraviolet rays, especially in situations where it is difficult to use topical sunscreen.

Hereinafter, the present invention will be described in more detail through specific examples. The following examples are merely examples to aid understanding of the present invention, and the scope of the present invention is not limited thereto.

Example

1. Preparation of active ingredients of oral administration composition

(1) Preparation of extract

Grape seed extract, green tea extract, and bisuri (ya gwanmun) extract were obtained by commercially purchasing from Shaanxi Jiahe Phytochem co., Ltd, Anhui Redstar Pharmaceutical Co., Ltd, and Haram Co., Ltd., respectively.

(2) Preparation of oral administration composition samples

Five samples of the oral composition were prepared at a weight ratio as follows, so that the total weight was 0.1 g.

Sample 1: Green tea:Bisuri:Grape seed = 1:1:1

Sample 2: Green tea:Bisuri:Grape seed = 2:1:1

Sample 3: Green tea:Bisuri:Grape seed = 1:2:1

Sample 4: Green tea:Bisuri:Grape seed = 3:1:1

Sample 5: Green tea:Bisuri:Grape seed = 1:1:3

0.1 g of each sample obtained in this way was weighed and dissolved in 10 mL DMSO to make 10 ㎍/mL, then diluted according to concentration and used in the experiment.

2. Antioxidant ability evaluation

To evaluate the antioxidant ability of the samples, experiments were conducted to confirm DPPH radical scavenging activity and ABTS radical scavenging activity.

(1) DPPH radical scavenging activity

DPPH (1,1-diphenyl-2-picrylhydrazyl), a stable free radical, exhibits maximum wavelength absorption at 517 nm. When it encounters a substance with antioxidant activity, DPPH is reduced and changes from dark purple to light yellow, and is generally free. Molecules with greater reactivity with radicals scavenge DPPH radicals more effectively. The DPPH sample was dissolved in methanol to prepare a 0.2 mM solution, and each single sample and mixed sample were prepared by concentration using DMSO. Ascorbic acid dissolved in methanol was used as a positive control, and 50 μL of each sample and 150 μL of DPPH solution were mixed in a 96 well plate and reacted in the dark at 25°C for 30 minutes. Absorbance was measured with a microplate reader at 517 nm, and DPPH scavenging activity was calculated by dividing the difference in absorbance between the control and experimental groups by the absorbance of the control group and converting it into a percentage according to the formula below.

DPPH free radical scavenging activity (%) =

(D - C) - (B - A) / C × 100

A: Absorbance before reaction of experimental group

B: Absorbance after reaction of experimental group

C: Absorbance before reaction of control group

D: Absorbance after reaction of control group

DPPH (2,2-diphenyl-1-picrylhydrazyl) is a dark purple compound whose antioxidant efficacy was measured by the extent to which it discolors to yellow by scavenging free radicals. Ascorbic acid, a positive control, was dissolved in methanol and the results of the experiment were shown in Figure 1 at concentrations of 50 - 3.125 ㎍/mL.

(2) ABTS radical scavenging activity

ABTS+ reagent is dark blue-green in color and changes to transparent white when it comes into contact with a substance with antioxidant activity. ABTS samples are dissolved in PBS to prepare 7.4 mM, and potassium persulfate is dissolved in PBS to make 5 mM potassium persulfate. The two solutions were mixed in a 1:1 ratio. Afterwards, it was wrapped in foil and reacted at room temperature for 24 h to prepare ABTS+. The sample reacted for 24 h was diluted with PBS so that the absorbance was 0.7 to 0.75 at 732 nm using a microplate reader. Each sample was diluted with DMSO, and ascorbic acid dissolved in DMSO was used as a positive control. 20 μL of each sample and 180 μL of ABTS+ solution were mixed in a 96-well plate and reacted in the dark at 25°C for 10 minutes. The absorbance was measured with a microplate reader at 732 nm, and the ABTS+ scavenging activity was calculated by dividing the difference in absorbance between the control and experimental groups by the absorbance of the control group and converting it into a percentage according to the formula below. The results are shown in Figure 1.

ABTS free radical scavenging activity (%) =

(D - C) - (B - A) / C × 100

A: Absorbance before reaction of experimental group

B: Absorbance after reaction of experimental group

C: Absorbance before reaction of control group

D: Absorbance after reaction of control group

ABTS is a dark blue-green compound whose antioxidant efficacy was measured by the degree to which it turns transparent white by scavenging free radicals. Ascorbic acid, a positive control, was dissolved in DMSO and the results of the experiment at a concentration of 50 - 1.5625 ㎍/mL are shown in Figure 2.

3. Evaluation of response to ultraviolet light exposure after oral administration of an orally administered composition

(1) Preparation of oral administration composition

In the composition shown in Table 1 below, 847 mg of crystalline cellulose, 14 mg of seaweed calcium, and 14 mg of calcium carboxymethyl cellulose were mixed in a mixer, and then mixed with 3 mg of magnesium stearate and 2 mg of silicon dioxide, and then tabletted and coated for oral administration. A composition was prepared. Hereinafter referred to as 'tablet of the present invention'.

ingredient content Hyaluronic acid 240mg green tea extract 200 mg Bisuri Extract 100mg Grape Seed Extract 100mg beta carotene 0.12mg Vitamin C 100mg Vitamin D 100IU Vitamin E 2.73mg Nicotinic acid amide 3.76mgNE Selenium 20mcg

(2)Clinical research

This study utilized two light sources (Excimer laser, Narrowband UVB) to expose the backs of 20 subjects over 18 years of age (Fitzpatrick skin types III, IV) to ultraviolet rays, and on Day 0, 308 nm was applied to the upper left back of the subjects. -Excimer laser (Xtrac, Photomedex, Montgomeryville, PA) is irradiated at 6 locations at 150, 200, 250, 300, 350, 400 (mJ/cm ² ), and narrowband UVB is applied to the lower left back at 150, 200, 250, 300, Investigate 6 locations at 350, 400 (mJ/cm2). Evaluate each evaluation item after 24 hours (Day 1). This evaluation result is referred to as ‘Pre-MI’.

Starting from the day after the evaluation, take 2 tablets of the present invention per day for 4 weeks (Day 2-29). On Day 29, 308nm excimer laser was irradiated to the subject's upper right back at 6 locations at 150, 200, 250, 300, 350, 400 (mJ/cm ² ), and narrowband UVB was irradiated to the subject's lower right back at 150, 200, 250, 300, 350, 400 (mJ/cm ² ) Investigate in 6 locations. Evaluate each evaluation item after 24 hours (Day 16). This evaluation result is referred to as 'Post-MI'.

Each evaluation item is ①Investigator's global assessment (IGA) evaluation, ②Transepidermal water loss (TEWL) evaluation, ③Erythema Index (EI), Melanin Index (MI) evaluation, ④Skin hydration evaluation, ⑤Minimal erythema dose (MED) evaluation, and ⑥Safety evaluation. , The detailed evaluation method is as follows:

① IGA (Investigator's global assessment) - subjective evaluation

IGA was evaluated according to the standards in Table 2 below. The primary effectiveness evaluation item is to check the mean value of IGA (adding up the IGA values of all 12 zones and dividing by 12).

As a secondary validity evaluation item, paired t-test was used to compare significant differences in left and right back MI, EI, TEWL (values of all 12 regions divided by 12), and MED values. You can. However, if the variances are not equal in the variance equality test, the Wilcoxon signed-rank test is used. The IGA (Investigator's global assessment) evaluation results are shown in Figure 3.

IGA severity Features 0 Clear of erythema One Trace erythema 2 Visible, not confluent erythema, no sharp borders 3 Confluent erythema with 4 sharp borders 4 Intense erythema

② TEWL measurement (Transepidermal water loss) - skin barrier

In a room with constant temperature (20-24℃) and humidity (28-38%), measure the Tewameter ^® twice by attaching it to your back, calculate the average value, and calculate the trans epidermal water loss (TEWL) measurement results. It is shown in Figure 4.

③ EI (Erythema index) and MI (Melasma index) measurement - erythema and pigmentation

In a room with constant temperature (20-24°C) and humidity (28-38%), the Mexameter ^® was placed in close contact with the back, and the average value was calculated after measuring twice. The results are shown in Figures 5 and 6, respectively.

In more detail, Figures 5 and 6 show the Mexameter ^® , a sensor probe with 16 light sources in three different wavelength bands (green 568㎚, red 660㎚, infrared 880㎚) corresponding to melanin and hemoglobin arranged in a circle. The measured erythema index (EI) and melanin index (MI), which are pigment indices, are measured using the results, respectively. The unit at this time is the arbitrary unit (aribiturary unit (AU)).

④ Measurement of skin hydration

In a room with constant temperature (20-24°C) and humidity (28-38%), the Corneometer ^® was placed close to the back and measured twice, the average value was calculated, and the results are shown in Figure 7. More specifically, the Corneometer ^® is a device that measures the water content within a depth of 30 to 40㎛ in the stratum corneum, and the resulting 'water content' is shown in Figure 7, where the unit is an arbitrary unit (AU). .

⑤ MED (Minimal erythema dose) evaluation - Minimum erythema dose

Minimum Erythema Dose (MED) refers to the minimum amount of UV radiation that can cause erythema to appear in the entire irradiated area within the range of 16 to 24 hours after UVB is irradiated to human skin. IGA severity 1 (trace erythema). The results are shown in Figure 8, and related photos are shown in Figure 9.

⑥ Check for adverse reactions

Observe any abnormal physical reactions that occur during the study period.

(3)Clinical research results

Significantly improved results were confirmed in all six indicators in 3.(2) above. In more detail, the minimum amount of erythema increased by 10.3%, erythema decreased by 14.7%, pigment decreased by 15.2%, skin barrier improved by 21.6%, skin moisture increased by 24.3%, and subjective evaluation also confirmed a 23.2% improvement. It has been done.

Although the embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and variations are possible without departing from the technical spirit of the present invention as set forth in the claims. This will be self-evident to those with ordinary knowledge in the field.

Claims (15)

Nicotinic acid amide; beta carotene; vitamin; selenium; hyaluronic acid; and
Contains as active ingredients an extraction mixture comprising green tea extract, bisuri extract and grape seed extract,
The extraction mixture includes green tea extract, bisuri extract, and grape seed extract in a weight ratio of 2:1:1, 3:1:1, or 1:1:3,
A composition for preventing, improving or treating skin damage caused by ultraviolet rays by antioxidant, suppressing skin moisture loss and suppressing erythema.
The method of claim 1, wherein the extraction mixture includes 20 to 60% by weight of green tea extract, 10 to 30% by weight of bisuri extract, and 20 to 60% by weight of grape seed extract, based on the total weight of the extraction mixture. , composition for improvement or treatment.
delete delete The composition for preventing, improving or treating skin damage caused by ultraviolet rays according to claim 1, wherein the vitamin further includes at least one vitamin component selected from the group consisting of vitamin C, vitamin D and vitamin E.
The composition for preventing, improving or treating skin damage caused by ultraviolet rays according to claim 1, which is a composition for oral administration for preventing or improving skin damage.
The composition for preventing, improving or treating skin damage caused by ultraviolet rays according to claim 6, wherein the composition for oral administration is a pharmaceutical composition for oral administration.
The method of claim 7, wherein the pharmaceutical composition for oral administration for preventing, improving or treating skin damage caused by ultraviolet rays is for preventing skin damage caused by ultraviolet rays, improving skin damage caused by ultraviolet rays, or occurring or worsening skin damage caused by ultraviolet rays. A composition for preventing, improving or treating skin damage caused by ultraviolet rays, which is used to treat skin diseases.
The composition for preventing, improving or treating skin damage caused by ultraviolet rays according to claim 8, wherein the skin disease is at least one skin disease selected from the group consisting of sunlight allergy, photosensitivity dermatitis, and melasma.
The method of claim 7, wherein the pharmaceutical composition for oral administration for preventing, improving or treating skin damage caused by ultraviolet rays is formulated with at least one solid preparation selected from the group consisting of tablets, pills, powders, granules, and capsules. A composition for preventing, improving or treating skin damage caused by ultraviolet rays.
The composition for preventing, improving or treating skin damage caused by ultraviolet rays according to claim 10, wherein the solid preparation contains 10 to 40% by weight of the active ingredient based on the total weight of the solid preparation.
The composition for preventing, improving or treating skin damage caused by ultraviolet rays according to claim 1, wherein the composition is an external composition for preventing, improving or treating skin damage caused by ultraviolet rays.
Nicotinic acid amide; beta carotene; vitamin; selenium; hyaluronic acid; and
Contains as active ingredients an extraction mixture comprising green tea extract, bisuri extract and grape seed extract,
The extraction mixture includes green tea extract, bisuri extract, and grape seed extract in a weight ratio of 2:1:1, 3:1:1, or 1:1:3,
A cosmetic composition for improving skin damage caused by ultraviolet rays by anti-oxidation, suppressing skin moisture loss, and suppressing erythema.
Nicotinic acid amide; beta carotene; vitamin; selenium; hyaluronic acid; and
Contains as active ingredients an extraction mixture comprising green tea extract, bisuri extract and grape seed extract,
The extraction mixture includes green tea extract, bisuri extract, and grape seed extract in a weight ratio of 2:1:1, 3:1:1, or 1:1:3,
A health functional food composition for improving skin damage caused by ultraviolet rays by anti-oxidation, suppressing skin moisture loss, and suppressing erythema.
Nicotinic acid amide; beta carotene; vitamin; selenium; hyaluronic acid; and
Contains as active ingredients an extraction mixture comprising green tea extract, bisuri extract and grape seed extract,
The extraction mixture includes green tea extract, bisuri extract, and grape seed extract in a weight ratio of 2:1:1, 3:1:1, or 1:1:3,
A health supplement composition for improving skin damage caused by ultraviolet rays by anti-oxidation, suppressing skin moisture loss, and suppressing erythema.

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