KR102585880B1 - A cosmetic composition for promoting skin beneficial bacteria - Google Patents

Abstract

The present invention promotes beneficial skin bacteria comprising as an active ingredient one or more substances selected from the group consisting of centella asiatica extract, oat extract, arnica extract, allantoin, black ginseng extract or fractions thereof, red ginseng extract or fractions thereof, tyrosine, and combinations thereof. It relates to a composition for use.
Centella asiatica extract, oat extract, arnica extract, allantoin, black ginseng extract or fractions thereof, red ginseng extract or fractions thereof, tyrosine, and combinations thereof according to the present invention exhibit an effect of promoting beneficial skin bacteria, and are therefore used as cosmetics or pharmaceutical compositions for promoting beneficial skin bacteria. can be provided.

Description

Cosmetic composition for promoting skin beneficial bacteria {A cosmetic composition for promoting skin beneficial bacteria}

The present invention relates to one or more substances selected from the group consisting of Centella asiatica extract or fractions thereof, oat extract or fractions thereof, arnica extract or fractions thereof, allantoin, black ginseng extract or fractions thereof, red ginseng extract or fractions thereof, tyrosine, and combinations thereof. It relates to a composition for promoting beneficial skin bacteria containing as an active ingredient. The composition may be provided as a cosmetic or pharmaceutical product.

Proper functioning of the skin barrier is essential for human health (Prescott et al. World Allergy Organization Journal (2017) 10:29). Keratinocytes in the stratum corneum, a component of the skin barrier, play an important role in the adaptation of the entire body by producing hormones, neurotransmitters and cytokines when the external environment changes. In this role, skin microbes help keratinocytes mature and influence the regulation of the body's entire immune system. Therefore, changes in microorganisms in the skin can cause not only skin diseases but also other inflammatory diseases (non-communicable diseases, NCDs). In fact, it is known that microbial disturbance in the stratum corneum is associated with skin allergies, psoriasis, flushing, acne, and skin aging.

The strains that mainly inhabit human skin are four species: Firmicutes , Bacteroidetes , Proteobacteria , and Actinobacteria . However, even within a single individual, the composition of skin microorganisms varies greatly depending on the environment. For example, areas with a lot of oil inhabit a lot of Propionibacteria and Staphylococci species, areas with a lot of moisture inhabit Corynebacteria and Staphylococcus species, and dry areas have several species of beta. -Proteobacteria ( beta-Proteobacteria ) and Flavobacteriale ( Flavobacteriale ) species mainly inhabit. In this way, the strains that inhabit the skin are different for each individual and may vary depending on each individual's nutritional status, health, age, and surrounding environment.

Among these , Staphylococcus epidermidis constitutes more than 90% of the aerobic environment. This bacterium has anti-inflammatory properties and helps act as a barrier that prevents the growth of pathogenic bacteria, such as Staphylococcus aureus strains. The mechanism of action of Stephylococcus epidermidis is to produce anti-bacterial peptides, bacteriocins, to prevent the production of cytokines, which are inflammatory substances, and to strengthen the skin barrier by supporting the expression of tight junction proteins. It helps with immunity. These actions are mediated by innate immune receptors on keratinocytes and other immune cells. Therefore, like other organs, the innate immune system of the skin can be said to be formed due to the interaction between humans and microorganisms, which is an important factor in the homeostatic control of skin immunity.

Accordingly, if the growth of Staphylococcus epidermidis, a skin flora and beneficial bacteria, can be promoted, the skin condition can be improved, and there is an increasing demand for the development of substances that can exhibit this beneficial bacteria promoting effect as cosmetic ingredients. .

Against this background, the present inventors made diligent efforts to develop a composition that promotes beneficial skin bacteria such as Staphylococcus epidermidis, and as a result identified natural products that have an effect of promoting beneficial skin bacteria and completed the present invention.

The object of the present invention is any one selected from the group consisting of Centella asiatica extract or fractions thereof, oat extract or fractions thereof, arnica extract or fractions thereof, allantoin, black ginseng extract or fractions thereof, red ginseng extract or fractions thereof, tyrosine, and combinations thereof. The object is to provide a composition for promoting beneficial skin bacteria containing the above as active ingredients.

Another object of the present invention is to provide a cosmetic composition containing the above composition.

Another object of the present invention is to provide a pharmaceutical composition for promoting beneficial skin bacteria comprising the composition.

This is explained in detail as follows. Meanwhile, each description and embodiment disclosed in the present invention may also be applied to each other description and embodiment. That is, all combinations of the various elements disclosed in the present invention fall within the scope of the present invention. Additionally, the scope of the present invention cannot be considered limited by the specific description described below.

One aspect of the present invention for achieving the above object is Centella asiatica extract or fractions thereof, oat extract or fractions thereof, arnica extract or fractions thereof, allantoin, black ginseng extract or fractions thereof, red ginseng extract or fractions thereof, tyrosine, and combinations thereof. Provided is a composition for promoting beneficial skin bacteria, comprising as an active ingredient at least one selected from the group consisting of.

The composition may contain 1 or more, 2 or more, 3 or more, 4 or more, 5 or more, or 6 of the above six substances, but is not limited thereto.

In one embodiment, the composition may include Centella asiatica extract or a fraction thereof, but is not limited thereto.

In one embodiment, the composition may include oat extract or a fraction thereof, but is not limited thereto.

In one embodiment, the composition may include arnica extract or a fraction thereof, but is not limited thereto.

In one embodiment, the composition may include allantoin, but is not limited thereto.

In one embodiment, the composition may include black ginseng extract or a fraction thereof, but is not limited thereto.

In one embodiment, the composition may include red ginseng extract or a fraction thereof, but is not limited thereto.

In one embodiment, the composition may contain tyrosine, but is not limited thereto.

The term " Centella asiatica " in the present invention is a perennial herb of the Umbrella Apiaceae family of the Ipanhwa group. In the present invention, the centella asiatica can be used in whole plants including roots, stems, leaves, fruits, flowers, and dried or processed products thereof, and in particular, leaf parts can be used, but is not limited thereto. The term "oats" of the present invention ( Avena sativa , Oat) is a biennial herb from the plant family, which is eaten cooked, used as a raw material for alcohol and snacks, or as feed for livestock. The kernel of oats is named oat kernel ( Avena sativa kernel). In the present invention, the oats can be used as whole plants including roots, stems, leaves, fruits (grains), flowers, and dried or processed products thereof. In particular, oat kernels, which are grains, can be used, but are not limited thereto.

The term "Arnica ( Arnica montana )" in the present invention is a perennial plant belonging to the Asteraceae family, and the components contained in its flowers are known to facilitate blood circulation. In the present invention, the arnica can be used as whole plants, including roots, stems, leaves, fruits, and flowers, and dried or processed products thereof, and in particular, flower parts can be used, but is not limited thereto.

The term "Allantoin" in the present invention is a natural substance extracted from the perennial grass comfrey, oak, wheat sprouts, etc., and is also called 5-ureidohydantoin. Ureide of glyoxylic acid It is a compound.

The term "black ginseng" of the present invention refers to ginseng that has turned black after several steaming and drying processes. Not only does the basically existing ginseng saponin not disappear through several growth and drying processes. In addition, it is known that the content of ginseng saponin, which only exists in processed ginseng, has been increased. In the present invention, the black ginseng may be black ginseng itself or fermented black ginseng, but is not limited thereto.

The fermented black ginseng may be manufactured by fermenting with a strain that can be used in conventional fermentation. The strain may be used without limitation as long as it is a strain used for fermentation known in the art, and may be, for example, lactic acid bacteria, yeast, or mold.

Specifically, the strains include Bifidobacterium genus strains, Lactobacillus genus strains, Lactococcus genus strains, Leuconostoc genus strains, Pediococcus genus strains, and Strepto. Lactic acid bacteria such as strains of the genus Streptococcus ; Saccharomyces genus strains, Shizosaccharomyces genus strains, Hygosaccharomyces genus strains, Hansenula genus strains, Brettanomyces genus strains, Decera genus strains, Streptomyces genus strains, etc. leaven; Or it may be a mold such as an Aspergillus genus strain or a Monascus genus strain, and may be a Lactobacillus genus or a Saccharomyces genus strain, but is not limited thereto.

More specifically, the strain is Bifidobacterium bifidum, Bifidobacterium breve , Bifidobacterium longum , Bifidobacterium animalis , Bifidobacterium Bifidobacterium lactis, Lactobacillus acidophilus, Lactobacillus casei , Lactobacillus gasseri , Lactobacillus delbrueckii spp . , Lactobacillus bulgaricus , Lactobacillus helveticus, Lactobacillus fermentum , Lactobacillus paracasei, Lactobacillus plantarum , Lactobacillus Terry ( Lactobacillus reuteri ), Lactobacillus rhamnosus ( Lactobacillus rhamnosus ), Lactobacillus salivarius ( Lactobacillus salivarius ), Lactococcus lactis, Leuconostoc mesenteroides ( Leuconostoc mesenteroides ), Pediococcus cerevi Lactic acid bacteria such as Pediococcus cerevisiae , Pediococcus acidilactici , Streptococcus lactis, Streptococcus thermophiles, and Streptococcus cremoris ; Saccharomyces cerevisiae, Saccharomyces ellipsoideus, Saccharomyces coreanus , Saccharomyces carlsbergensis, Saccharomyces carlsbergensis Saccharomyces pastorianus , Saccharomyces lactis, Saccharomyces rouxii , Schizosaccharomyces pombe , Zygosaccharomyces major ), Hansenula anomala , Brettanomyces bruxellensis , Brettanomyces custersianus , Dekkera anomala , Streptomyces oliboque Yeast such as Streptomyces olivochromogenes and Streptomyces griseus ; Or Aspergillus orizae, Aspergillus sojae , Monascus albidulus , Monascus pilosus , Monascus purpureus , etc. Mold; can be used alone or in combination. The strain may be an isolated strain or a commercially available strain without limitation.

In the present invention, when the black ginseng is fermented black ginseng, the filtrate or unfiltered product of the fermented black ginseng extract can be used, but is not limited thereto.

The term “filtrate” in the present invention refers to material from which sediment or particles have been filtered. The method of obtaining the filtrate is not particularly limited, and filtration may be performed using methods known in the art, for example, membranes, filters, etc.

The term "ferment" in the present invention refers to a material that has not been filtered, but does not exclude unnecessary components contained in the material, such as bacteria or proteins, that have been removed by methods such as centrifugation and precipitation. .The filtrate or unfiltered product may be a filtrate or unfiltered product of fermented material, but is not limited thereto.

The term "Red ginseng" of the present invention refers to ginseng that has turned red by applying a special steaming, drying and processing process to fresh ginseng. Due to changes in composition due to heat treatment, red ginseng contains ginsenosides and antioxidant active substances. It contains a large amount compared to fresh or white ginseng, and is known to produce unique ginsenosides. In the present invention, the red ginseng may be red ginseng itself or fermented red ginseng, but is not limited thereto.

The fermented red ginseng may be manufactured by fermenting with a strain that can be used in conventional fermentation. The strain may be used without limitation as long as it is a strain used for fermentation known in the art, and may be, for example, lactic acid bacteria, yeast, or mold. The strain is as described above.

In the present invention, when the red ginseng is fermented red ginseng, the filtrate or unfiltered product of the fermented red ginseng extract can be used, but is not limited thereto.

The term "Tyrosine" in the present invention refers to an aromatic amino acid that has a benzene ring in the molecule as a non-essential amino acid. The tyrosine may be either D-type or L-type, and for the purpose of the present invention, L-type is preferred, but is not limited thereto.

The term "extract" in the present invention refers to a result such as a liquid component obtained by immersing the desired substance in various solvents and then extracting it at room temperature, low temperature, or heated state for a certain period of time, and a solid component obtained by removing the solvent from the liquid component. means. In addition, in addition to the above results, it can be comprehensively interpreted to include all dilutions of the results, concentrates thereof, crude preparations, purifications, etc. of the above results.

In the present invention, the extract may be centella asiatica extract, oat extract, arnica extract, black ginseng extract, or red ginseng extract. The extract can be extracted from various organs of natural, hybrid, and mutated plants of the target plant, for example, roots, aerial parts, stems, leaves, flowers, fruit bodies, fruit skins, as well as plant tissues, It can be appropriately selected depending on the type of target plant, but is not limited to this. The extract can be obtained by extraction with water or various organic solvents. At this time, the organic solvent used is not particularly limited as long as it can obtain an extract, but may specifically be water, a polar solvent, or a non-polar solvent, and more specifically, water, a lower alcohol having 1 to 6 carbon atoms ( It may be a C1 to C6 alcohol, such as methanol, ethanol, propanol, or butanol) or a mixed solvent thereof.

In addition, the method for obtaining the extract is not particularly limited as long as the extract can be obtained, but specifically, the roots, stems, leaves, fruits, flowers, and Methods such as cold immersion extraction, heating extraction, extraction by applying ultrasonic waves, and reflux extraction using a reflux condenser can be used by immersing dried products, processed products, etc. in the above solvent and extracting at room temperature.

The term “fraction” in the present invention refers to the result obtained by performing fractionation to separate a specific component or specific group of components from a mixture containing various components. In the present invention, the fraction may be a fraction of Centella asiatica extract, a fraction of an oat extract, a fraction of an arnica extract, a fraction of a black ginseng extract, or a fraction of a red ginseng extract.

The fractionation method for obtaining the fraction in the present invention is not particularly limited as long as the fraction can exhibit an effect of promoting beneficial skin bacteria, but may be performed according to a method commonly used in the art. For example, solvent fractionation performed by treatment with various solvents, ultrafiltration fractionation performed by passing ultrafiltration membranes with a certain molecular weight cut-off value, and various chromatographic methods (separation based on size, charge, hydrophobicity, or affinity). It can be a chromatographic fractionation method that performs (produced for), and a combination thereof.

The type of fractionation solvent used to obtain the fraction in the present invention is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the fractionating solvent include polar solvents such as water, distilled water, and alcohol; Nonpolar solvents such as hexane, ethyl acetate, chloroform, and dichloromethane may be included. These may be used individually or in combination of two or more types. When alcohol is used among the fractionating solvents, lower alcohols having 1 to 4 carbon atoms (C1 to C4 alcohols) can be used.

The above centella asiatica, oats, arnica, black ginseng, and red ginseng can be used without limitation, whether cultivated or commercially available.

The composition of the present invention is any selected from the group consisting of Centella asiatica extract or fractions thereof, oat extract or fractions thereof, arnica extract or fractions thereof, allantoin, black ginseng extract or fractions thereof, red ginseng extract or fractions thereof, tyrosine, and combinations thereof. One or more substances may be included in an amount of 0.0005 to 90% by weight (w/w), specifically 0.001 to 30% by weight (w/w), based on the total weight of the composition, but are not limited thereto.

The term "skin beneficial bacteria" of the present invention refers to strains that help relieve inflammation, control psoriasis-related inflammation, inhibit the growth of harmful bacteria, and inhibit tumor growth among skin flora. The skin beneficial bacteria may be, for example, Staphylococcus epidermidis , but are not limited thereto. The Staphylococcus epidermidis improves skin defense by inducing the expression of antimicrobial peptide (AMP) from skin epidermal cells and enhancing skin regeneration effect; When treated with PSM (Phenol soluble modulin), a component secreted by Stephylococcus epidermidis, it promotes skin regeneration by inhibiting the destruction (death) of cell membranes and growth of harmful bacteria; Butyric acid, a component secreted by Stephylococcus epidermidis, suppresses the expression of inflammatory factors induced by ultraviolet rays, showing a skin soothing effect; When applying live bacteria to the skin, it has the effect of increasing skin moisture and suppressing moisture loss; When live bacteria are applied to the skin, the pH of the skin becomes slightly acidic and the pH environment can be improved; therefore, substances that have an effect of promoting the beneficial bacteria, Staphylococcus epidermidis, have a beneficial effect as a cosmetic composition. The "harmful bacteria" refers to bacteria that cause inflammatory reactions or acne on the skin, for example, Propionibacterium acnes , Corynebacterium minutissimum , and Stephylococcus aureus. It may be Staphlococcus aureus , etc., but is not limited thereto.

The term "enhancing skin beneficial bacteria" in the present invention means promoting or improving the growth of the above-mentioned skin beneficial bacteria. For the purpose of the present invention, the promotion of beneficial skin bacteria may be the promotion of Staphylococcus epidermidis, but is not limited thereto.

Another aspect of the present invention provides a cosmetic composition containing the composition.

In the present invention, the cosmetic composition includes a solution, external ointment, cream, foam, nutritional lotion, softening lotion, pack, softening water, emulsion, makeup base, essence, soap, liquid cleanser, bath salt, sunscreen cream, sun oil, Can be prepared in a formulation selected from the group consisting of suspensions, emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansers, oils, powder foundations, emulsion foundations, wax foundations, patches and sprays. Not limited.

In the present invention, the cosmetic composition may further include one or more cosmetically acceptable carriers that are blended with general skin cosmetics, and common ingredients include, for example, oil, water, surfactant, moisturizer, and lower alcohol. , thickeners, chelating agents, pigments, preservatives, fragrances, etc. may be appropriately mixed, but are not limited thereto.

Cosmetically acceptable carriers included in the cosmetic composition of the present invention vary depending on the formulation of the cosmetic composition.

When the formulation of the present invention is an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc. are used as carrier ingredients. may be used, but is not limited to this. These may be used individually or in combination of two or more types.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silcate, polyamide powder, etc. may be used as carrier ingredients, and especially in the case of a spray, chlorofluorohydride may be additionally used. May include, but are not limited to, propellants such as low carbon, propane/butane or dimethyl ether. These may be used individually or in combination of two or more types.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent, or emulsifying agent may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil, sesame oil, glycerol aliphatic esters, polyethylene glycol, or fatty acid esters of sorbitan may be used, but are limited to these. It doesn't work. These may be used individually or in combination of two or more types.

When the formulation of the present invention is a suspension, the carrier components include water, liquid diluents such as ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, and miso. Crystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used, but are not limited thereto. These may be used individually or in combination of two or more types.

When the formulation of the present invention is a soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolyzates, isethionates, lanolin derivatives, fatty alcohols, vegetable oils, glycerol, sugars, etc. may be used as carrier ingredients. may, but is not limited to this. These may be used individually or in combination of two or more types.

The cosmetic composition of the present invention is selected from the group consisting of Centella asiatica extract or fractions thereof, oat extract or fractions thereof, arnica extract or fractions thereof, allantoin, black ginseng extract or fractions thereof, red ginseng extract or fractions thereof, tyrosine, and combinations thereof. One or more substances may be included in an amount of 0.0005 to 90% by weight (w/w) based on the total weight of the composition, and specifically may be included in an amount of 0.001 to 30% by weight (w/w), but is not limited thereto.

Another aspect of the present invention provides a pharmaceutical composition for promoting beneficial skin bacteria, comprising the composition.

The pharmaceutical composition of the present invention may further include a pharmaceutically acceptable carrier, excipient, or diluent commonly used in the preparation of the pharmaceutical composition, and the carrier may include a non-naturally occurring carrier. . The carriers, excipients, and diluents include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, and microcrystalline. Examples include cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil.

In addition, the pharmaceutical composition can be manufactured into tablets, pills, powders, granules, capsules, suspensions, oral solutions, emulsions, syrups, sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and transdermal preparations according to conventional methods. It can be formulated and used in the form of an absorbent, gel, lotion, ointment, cream, patch, cataplasma, paste, spray, skin emulsion, skin suspension, transdermal delivery patch, drug-containing bandage, or suppository.

Specifically, when formulating, it may be prepared using commonly used diluents or excipients such as fillers, weighting agents, binders, wetting agents, disintegrants, and surfactants. Solid preparations for oral administration include, but are not limited to, tablets, pills, powders, granules, capsules, etc. These solid preparations may be prepared by mixing at least one excipient, such as starch, calcium carbonate, sucrose, lactose, gelatin, etc. Additionally, in addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. In addition to oral liquid and liquid paraffin, it can be prepared by adding various excipients, such as wetting agents, sweeteners, fragrances, and preservatives. Preparations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized preparations and suppositories. Non-aqueous solvents and suspensions may include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate. As a base for suppositories, wethepsol, macrogol, Tween 61, cacao, laurin, glycerogelatin, etc. can be used.

The pharmaceutical composition of the present invention is selected from the group consisting of Centella asiatica extract or fractions thereof, oat extract or fractions thereof, arnica extract or fractions thereof, allantoin, black ginseng extract or fractions thereof, red ginseng extract or fractions thereof, tyrosine, and combinations thereof. One or more substances may be included in an amount of 0.0005 to 90% by weight (w/w) based on the total weight of the composition, and specifically may be included in an amount of 0.001 to 30% by weight (w/w), but is not limited thereto.

The pharmaceutical composition of the present invention can be administered in a pharmaceutically effective amount. The term "pharmaceutically effective amount" means an amount sufficient to treat the disease with a reasonable benefit/risk ratio applicable to medical treatment, and the effective dose level is determined by the type and severity of the subject, age, sex, activity of the drug, It can be determined based on factors including sensitivity to the drug, time of administration, route of administration and excretion rate, duration of treatment, concurrently used drugs, and other factors well known in the field of medicine. For example, the pharmaceutical composition can be administered at a dose of 0.01 to 500 mg/kg per day, specifically 10 to 100 mg/kg, and the administration may be administered once a day or in divided doses.

The pharmaceutical composition may be administered as an individual therapeutic agent or in combination with other therapeutic agents, and may be administered sequentially or simultaneously with conventional therapeutic agents. And it can be administered single or multiple times. Considering all of the above factors, it is important to administer an amount that can achieve maximum effect with the minimum amount without side effects, and can be easily determined by a person skilled in the art.

In addition, the pharmaceutical composition can be administered orally or parenterally (e.g., intravenously, subcutaneously, intraperitoneally, or topically) depending on the desired method, and the dosage is determined by the patient's condition and weight, and the degree of the disease. , it varies depending on the drug form, administration route and time, but can be appropriately selected by a person skilled in the art.

In one embodiment of the present invention, it was confirmed that the Centella asiatica extract, oat kernel extract, arnica flower extract, allantoin, black ginseng extract, red ginseng extract, and/or tyrosine according to the present invention exhibit a skin beneficial bacteria promotion effect of up to 23.3%, 2 Not only was it confirmed that when mixing different materials, the effect of promoting beneficial bacteria increases compared to a single material, but also when mixing 7 types of materials, it was confirmed that the effect of promoting beneficial skin bacteria was up to 30.3%. Since it was confirmed that when mixing the above materials, they show a synergistic effect without attenuating each other, it can be applied as a composition for promoting beneficial skin bacteria.

Centella asiatica extract, oat extract, arnica extract, allantoin, black ginseng extract, red ginseng extract, tyrosine, and combinations thereof according to the present invention exhibit an effect of promoting beneficial skin bacteria, and can therefore be provided as a cosmetic composition or pharmaceutical composition for promoting beneficial skin bacteria.

Hereinafter, the present invention will be described in more detail by examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited by these examples, and it will be clear to those skilled in the art to which the present invention pertains.

Example 1. Selection of beneficial skin bacteria

Among various skin flora, Staphylococcus epidermidis ( S. epidermidis ) has the effects shown in Table 1 below, so it was selected as a skin beneficial bacteria and materials that increase it were selected in the examples below.

skin flora human effects mechanism of action indicator substance references S. epidermidis Relieve inflammation Regulates the FFAR2 (Free fatty acid receptor 2) mechanism to suppress cytokine enhancement caused by UVB Butyric acid One) Controls psoriasis-related inflammation Regulates the expression of A20, an inflammation-regulating protein in the host. N/A 2) Inhibition of tumor growth Selective inhibition of DNA polymerase activity in mesangial cells 6-N-hydroxyaminopurine (6-HAP) 3)

1) Butyric acid from probiotic　Staphylococcus epidermidis　in the skin microbiome down-regulates the ultraviolet-induced pro-inflammatory IL-6 cytokine via short-chain fatty acid receptor (2019) International Journal of Molecular sciences 20:4477.

2) Staphylococcus epidermidis-induced Interleukin-1 beta and human beta-defensin-2 expression in human keratinocytes is regulated by the host molecule A20 (TNFAIP3) (2019) Acta Dermato-Venereologica 99:181-187.

3) A commensal strain of Staphylococcus epidermidis protects against skin neoplasia (2018) Science Advances 4:eaao4502.

Example 2. Testing the effect of screening materials on promoting beneficial skin bacteria

Centella asiatica, oats, arnica, allantoin, black ginseng, red ginseng, and tyrosine were selected as materials capable of promoting beneficial skin bacteria and prepared as follows.

Specifically, Centella asiatica extract was obtained by drying and chopping Centella asiatica leaves (purchased from a medicinal product market), placing 100 g of dry weight in a flask, and extracting with 1000 g of extraction solvent (distilled water: ethanol = 3:7) at room temperature for 3 days. Centella asiatica extract was prepared by filtering the extract through a filter with a pore size of 0.2㎛.

The oat extract was made by drying and chopping oat kernels (purchased from a pharmaceutical market), placing 100 g of the dry weight in a flask, and extracting it with 1000 g of extraction solvent (distilled water: ethanol = 3:7) at room temperature for 3 days. The extract was filtered through a filter with a pore size of 0.2㎛ to prepare an oat kernel extract.

Arnica extract was made by drying and chopping Arnica flowers (purchased from a medicinal product market), placing 100 g of the dry weight in a flask, and extracting it with 1000 g of extraction solvent (distilled water: ethanol = 3:7) at room temperature for 3 days. Arnica flower extract was prepared by filtering the extract through a filter with a pore size of 0.2㎛.

For the black ginseng extract, black ginseng was dried and cut into pieces, then 100 g of the dry weight was placed in a flask and extracted with 1000 g of extraction solvent (distilled water: ethanol = 3:7) at room temperature for 3 days. The extract was filtered through a filter with a pore size of 0.2㎛ to prepare a black ginseng extract.

For the red ginseng extract, red ginseng was dried and cut into pieces, then 100 g of dry weight was placed in a flask and extracted with 1000 g of extraction solvent (distilled water: ethanol = 3:7) at room temperature for 3 days. A red ginseng extract was prepared by filtering the extract through a filter with a pore size of 0.2㎛.

Allantoin or tyrosine was used by purchasing commercially available reagents.

Sterile liquid medium was prepared using commercial TSB (Tryptic soy broth) medium. S. epidermidis glycerol-preserved solution was inoculated into sterile liquid TSB medium and pre-cultured at 30-37°C for 6-18 hours. This preculture was performed 1-2 times as needed. 0.1-10% of each of the previously prepared samples (centella extract, oat kernel extract, arnica flower extract, allantoin, black ginseng, red ginseng, and tyrosine) to be evaluated was added to the sterile liquid TSB medium, and the pre-culture solution was inoculated, Cultured at 30-37°C for 6-18 hours. Glucose was added as a positive control. After culturing, the absorbance immediately after inoculation and the absorbance during the stationary phase of growth were measured, and the ratio of the values (growth rate) was used as a statistical variable. The growth rate of the experimental group treated with the sample was compared with that of the control group that did not treat the sample, to determine whether the sample had a significant effect on the growth of bacteria. The evaluation was conducted at least 3-6 times, the average value and standard deviation of the growth rate of multiple samples were calculated, and significance was verified through t-test.

As a result, as shown in Table 2 below, centella asiatica extract, oat kernel extract, arnica flower extract, allantoin, black ginseng extract, red ginseng extract or tyrosine showed an effect of promoting skin beneficial bacteria by more than 4.5% and up to 23.3%.

Material Effect of promoting beneficial bacteria (%) Glucose (positive control) 12.5 Centella asiatica extract 15.2 Oat kernel extract 13.9 Arnica Flower Extract 23.3 allantoin 20.3 black ginseng extract 10.2 Red Ginseng Extract 6.8 tyrosine 4.5%

Example 3. Testing the effect of material mixture on promoting beneficial skin bacteria

The effect of promoting beneficial bacteria according to the combination of centella asiatica extract, oat kernel extract, arnica flower extract, allantoin, black ginseng extract, red ginseng extract, or tyrosine prepared in Example 2 was confirmed in the same manner as Example 2.

As a result, as shown in Table 3 below, when two or more types of materials were mixed, the effect of promoting beneficial bacteria was more than 15%, up to a maximum of 27.7%, and when seven types of materials were mixed in the same ratio, the effect was 30.3%, with seven types of When the materials were mixed at a ratio of extract:single ingredient = 2:1, a 28.8% beneficial bacteria promotion effect was observed.

material mixture Effect of promoting beneficial bacteria (%) material mixture Effect of promoting beneficial bacteria (%) Centella asiatica extract + arnica flower extract (same ratio) 23.7 Arnica flower extract + black ginseng extract (equal ratio) 26.8 Centella asiatica extract + allantoin (same ratio) 22.0 Arnica flower extract + red ginseng extract (same ratio) 26.8 Centella asiatica extract + black ginseng extract (same ratio) 15.8 Arnica flower extract + tyrosine (same ratio) 26.6 Centella asiatica extract + red ginseng extract (same ratio) 15.9 Allantoin + black ginseng extract (same ratio) 22.5 Centella asiatica extract + tyrosine (same ratio) 16.0 Allantoin + red ginseng extract (same ratio) 22.4 Oat kernel extract + Arnica flower extract (equal ratio) 25.0 Allantoin + Tyrosine (same ratio) 22.6 Oat kernel extract + allantoin (same ratio) 22.2 Black ginseng extract + red ginseng extract (same ratio) 15.7 Oat kernel extract + black ginseng extract (same ratio) 15.9 Black ginseng extract + tyrosine (same ratio) 12.3 Oat kernel extract + red ginseng (same ratio) 15.7 Red ginseng extract + tyrosine (same ratio) 12.7 Oat kernel extract + tyrosine (same ratio) 15.0 Mix of 7 types (equal ratio) 30.3 Arnica flower extract + allantoin (equal ratio) 27.7 Mix of 7 types (extract: single ingredient = 2:1 ratio) 28.8

From the results of the above examples, it was confirmed that centella asiatica extract, oat kernel extract, arnica flower extract, allantoin, black ginseng extract, red ginseng extract, or tyrosine showed up to 23.3% of the effect of promoting beneficial skin bacteria. In addition, it was confirmed that when mixing two types of materials, the effect of promoting beneficial bacteria increases compared to a single material, and when mixing seven types of materials, it was confirmed that the effect of promoting beneficial skin bacteria was up to 30.3%, showing that not only the single material but also the two It was confirmed that when mixing more than one type of material, it shows a synergistic effect without attenuating each other. These results suggest that the materials of the present invention can be applied alone or in combination as a composition for promoting beneficial skin bacteria.

The composition for promoting skin beneficial bacteria according to the present invention promotes Staphylococcus epidermidis, a skin beneficial bacteria, and Stephylococcus epidermidis induces the expression of antimicrobial peptide (AMP) from skin epidermal cells and has a skin regenerative effect. Improves skin defense by enhancing (Staphylococcus epidermidis: A potential new player in the physiopathology of acne? (2019) Dermatology 235:287-294); When treated with PSM (Phenol soluble modulin), a component secreted by Stephylococcus epidermidis, it promotes skin regeneration by inhibiting the destruction (death) of cell membranes and growth of harmful bacteria (Selective antimicrobial action is provided by phenol-soluble modulins derived from Staphylococcus epidermidis, a normal resident of the skin (2010) Journal of Investigative Dermatology 130:192-200); Butyric acid, a component secreted by Staphylococcus epidermidis, suppresses the expression of inflammatory factors induced by ultraviolet rays and has a skin soothing effect. pro-inflammatory IL-6 cytokine via short-chain fatty acid receptor (2019) International Journal of Molecular sciences 20:4477); When applying live bacteria to the skin, it has the effect of increasing skin moisture and suppressing moisture loss (Pilot study on novel skin care method by augmentation with Staphylococcus epidermidis, an autologous skin microbe - A blinded randomized clinical trial (2015) Journal of Dermatological Science 79: 119-126 (doi: 10.1016/j.jdermsci.2015.05.001)); When applying live bacteria to the skin, the pH of the skin becomes slightly acidic, improving the pH environment (Pilot study on novel skin care method by augmentation with Staphylococcus epidermidis, an autologous skin microbe - A blinded randomized clinical trial (2015) Journal of Dermatological Science 79: 119-126 (doi: 10.1016/j.jdermsci.2015.05.001)), it has a beneficial effect as a cosmetic composition.

From the above description, those skilled in the art to which the present invention pertains will understand that the present invention can be implemented in other specific forms without changing its technical idea or essential features. In this regard, the embodiments described above should be understood in all respects as illustrative and not restrictive. The scope of the present invention should be construed as including the meaning and scope of the claims described below rather than the detailed description above, and all changes or modified forms derived from the equivalent concepts thereof.

Claims (5)

Red ginseng extract or fractions thereof; and a composition for promoting beneficial skin bacteria, comprising tyrosine as an active ingredient.
The composition of claim 1, wherein the skin beneficial bacteria are Staphylococcus epidermidis .
delete A cosmetic composition comprising the composition of claim 1 or 2.
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