KR20240044148A - Cosmetic composition for anti-oxidative or anti-inflammatory comprising fermented extract of Moringa oleifera Leaf, Acorus calamus var. angustatus BESS. plant and Lycium chinense MILL. Fruit - Google Patents

Abstract

The present invention provides an antioxidant or anti-inflammatory agent comprising fermented extracts of Moringa oleifera (Leaf), Acorus calamus var. angustatus BESS. ( plant), and Lycium chinense MILL. ( Fruit) as active ingredients. It relates to a cosmetic composition for use.
The composition containing fermented extracts of Moringa oleifera (Leaf), Acorus calamus var. angustatus BESS. ( plant), and Lycium chinense MILL. ( Fruit) according to the present invention as active ingredients has antioxidant properties. It not only has excellent anti-inflammatory effects but also has almost no toxicity and side effects, so it can be effectively used in cosmetic compositions or external skin agents.

Description

Cosmetic composition for antioxidant or anti-inflammatory comprising fermented extract of Moringa oleifera Leaf, Acorus calamus var. angustatus BESS. plant and Lycium chinense MILL. Fruit}

The present invention provides an antioxidant or anti-inflammatory agent comprising fermented extracts of Moringa oleifera (Leaf), Acorus calamus var. angustatus BESS. ( plant), and Lycium chinense MILL. ( Fruit) as active ingredients. It relates to a cosmetic composition for use.

Active oxygen introduced from outside the body or generated within the body causes many problems, such as accelerating aging of the body or causing cancer. Therefore, much development and research is being conducted on antioxidant substances that inhibit oxidation by active oxygen. Antioxidants are widely distributed in the animal and plant kingdoms, and many phenolic compounds, flavonoids, tocopherols, vitamin C, and selenium are known to be present in fruits and vegetables. However, antioxidant substances that exist in nature cannot be expected to have a substantially sufficient effect when applied to the skin. Therefore, synthetic antioxidants that have excellent antioxidant power and are inexpensive are widely used, but their use is limited due to safety concerns such as side effects on the human body.

In addition, inflammation is the body's immune response to wounds or diseases, and oxidative stress such as ultraviolet rays, active oxygen, and free radicals activate inflammatory factors, causing various diseases and skin aging. Vasoactive polypeptides such as kinin, plasmin, and complement have vasodilation and constriction and chemotaxis effects, and lymphocytes such as interleukin-6 (IL-6) Phosphorus and arachidonic acid are responsible for the inflammatory response. Arachidonic acid is metabolized through two pathways, cyclooxygenase or lipooxygenase, into prostaglandins and leukotrienes, which are inflammatory mediators, and mediates various inflammatory reactions. Meanwhile, anti-inflammatory agents act to eliminate inflammation by removing the inflammatory source and reducing biological reactions and symptoms. Substances currently used for anti-inflammatory purposes include non-steroids such as flufenamic acid, ibuprofen, benzydamine, and indomethacin, and steroids such as prednisolone. , dexamethasone, etc. In addition, allantoin, Azn, hydrocortisone, etc. are known to be effective in anti-inflammatory, but the amount of use of these substances is limited due to safety issues on the skin, or the effect is so minimal that it is not possible to expect a practical effect of alleviating inflammation. There is.

Therefore, there is a need to develop materials of natural origin that have excellent antioxidant and anti-inflammatory effects while having no or low risk of side effects or cytotoxicity. In particular, recently, research and development on cosmetics or cosmetic compositions using natural raw materials has been actively conducted to meet the needs of consumers.

Prior literature

1. Registered Patent KR101845798B1

As a result of our diligent efforts to develop materials derived from natural products that are harmless to the human body and have antioxidant and anti-inflammatory effects, the present inventors have developed moringa leaves ( Moringa oleifera , Leaf), calamus whole plant ( Acorus calamus var. angustatus BESS., plant) and Goji berry. After confirming that the fermented extract of Lycium chinense MILL. ( Fruit) exhibits excellent radical scavenging activity as well as NO, TNF-a, IL-6, and COX-2 inhibitory activity, the present invention was completed.

The present invention provides an antioxidant or anti-inflammatory agent comprising fermented extracts of Moringa oleifera (Leaf), Acorus calamus var. angustatus BESS. ( plant), and Lycium chinense MILL. ( Fruit) as active ingredients. The purpose is to provide a cosmetic composition for use.

In the cosmetic composition according to the present invention, the fermented extract may be obtained by inoculating yeast into a medium containing dried moringa leaves, irises, and goji berries and then culturing them. The yeast may be Saccharomyces cerevisiae. The culturing may be performed at a temperature of 20°C to 40°C for 12 to 60 hours.

In the cosmetic composition according to the present invention, the weight ratio of Moringa oleifera (Leaf), Acorus calamus var. angustatus BESS., plant, and Lycium chinense MILL., Fruit is 1:1. :1.

In the cosmetic composition according to the present invention, the fermented extract may be contained at a concentration of 0.001 to 20 mM in the cosmetic composition.

In the cosmetic composition according to the present invention, the formulation of the cosmetic composition includes lotion (skin lotion), skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutritional lotion, massage cream, nutritional cream, eye cream, and moisture. One or more may be selected from the group consisting of cream, hand cream, foundation, essence, nutritional essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, and body cleanser.

The present invention also provides an antioxidant or anti-oxidant agent comprising fermented extracts of Moringa oleifera (Leaf), Acorus calamus var. angustatus BESS. ( plant), and Lycium chinense MILL. ( Fruit) as active ingredients. The purpose is to provide an external skin preparation for inflammation.

In the topical skin preparation according to the present invention, the fermented extract may be obtained by inoculating yeast into a medium containing dried moringa leaves, iris sprouts, and goji berry fruits and then culturing them. The yeast may be Saccharomyces cerevisiae. The culturing may be performed at a temperature of 20°C to 40°C for 12 to 60 hours.

In the skin external preparation according to the present invention, the weight ratio of Moringa oleifera (Leaf), Acorus calamus var. angustatus BESS. ( plant), and Lycium chinense MILL. ( Fruit) is 1:1. :1.

In the skin external preparation according to the present invention, the formulation of the external preparation may be one or more selected from the group consisting of creams, gels, patches, sprays, ointments, warning agents, lotions, liniment agents, pasta agents, and cataplasma agents. there is.

The composition containing fermented extracts of Moringa oleifera (Leaf), Acorus calamus var. angustatus BESS. ( plant), and Lycium chinense MILL. ( Fruit) according to the present invention as active ingredients has antioxidant properties. It is expected to be effectively used in cosmetic compositions or external skin agents because it not only has excellent anti-inflammatory effects but also has almost no toxicity and side effects.

Meanwhile, the scope of the present invention is not limited by the effects described above.

Figure 1 shows the DPPH scavenging activity of extracts according to Preparation Examples 1 to 7.
Figure 2 shows the NO inhibitory activity of extracts according to Preparation Examples 1 to 7.
Figure 3 shows the TNF-a inhibitory activity of extracts according to Preparation Examples 1 to 7.
Figure 4 shows the IL-6 inhibitory activity of extracts according to Preparation Examples 1 to 7.
Figure 5 shows the COX-2 inhibitory activity of extracts according to Preparation Examples 1 to 7.
Figure 6 shows the cytotoxicity of the mixed extract of moringa leaves, calamus whole plant, and goji berry fruit according to Preparation Example 7 (left: Raw264.7 cytotoxicity test results, right: HDP cytotoxicity test results).

Hereinafter, an antioxidant or anti-inflammatory cosmetic composition containing fermented extracts of moringa leaves, calamus whole plant, and goji berry fruit according to specific embodiments of the invention as active ingredients will be described in detail. However, this is presented as an example of the invention, and the scope of the invention is not limited thereby, and it is obvious to those skilled in the art that various modifications to the embodiment are possible within the scope of the invention. Throughout this specification, unless otherwise specified, “include” or “contains” refers to the inclusion of a certain component (or component) without particular limitation, excluding the addition of other components (or components). cannot be interpreted as

In the present invention, “Moringa leaf” refers to the leaf of the Moringa tree. Moringa tree is a plant of the Moringa Genus, Moringaceae Family, and its scientific name is Moringa Oleifera (synonym Moringa Pterygosperma), and the scientific name is derived from it. It is also called Murunggai in Tamil. Moringa is a legume, a tropical tree whose leaves and fruits as well as the entire tree are edible. It contains more than 90 types of nutrients, including large amounts of amino acids, minerals, and vitamins. In particular, moringa leaves prevent cholesterol, have anti-inflammatory properties, improve vision, increase energy, normalize blood pressure, restore skin health, improve digestive function, strengthen the immune system, prevent wrinkles/aging, improve wound healing, prevent tumors, normalize blood sugar, and prevent ulcers. Moringa plant contains more than 46 antioxidants and 36 anti-inflammatory compounds.

In the present invention, “Calamus Outpost” belongs to the Araceae family and contains essential oil components such as eugenol, asarylaldehyde, and asarone, tannin, axorin, It contains ingredients such as palmitic acid and is known to have sedative, analgesic, antispasmodic, antitussive and expectorant effects.

In the present invention, “Goji berry” is a woody plant in the leaves of the Goji berry of the Solanaceae family and is a herbal medicine that grows or is cultivated in Korea, China, Taiwan, Japan, Europe, etc. The fruits, leaves, and roots of goji berry are used for each part, and the wolfberry fruits, leaves, and roots contain substances such as betaine, rutin, kukoamine A, and -sitosterol. Contains a large amount of functional ingredients.

The term “skin” used herein refers to the tissue that covers the body surface of an animal, and is the broadest concept that includes not only the tissue that covers the body surface of the face or body, but also the scalp and hair.

As used herein, the term “fermentation” refers to any activity or process involving enzymatic or metabolic decomposition of organic substances using microorganisms. The microorganism may be at least one selected from the group consisting of yeast, lactic acid bacteria, bacteria, and mold. The yeast is, for example, Saccharomyces cerevisiae, S. ellipsoideus , S. coreanus , Saccharomyces carlsbergensis ( S. carlsbergensis ), Saccharomyces pastorianus ( S. pastorianus ), Saccharomyces lactis ( S. lactis ), S. rouxii , Schizosaccharomyces pombe ), Zygosaccharomyces major , Hansenula anomala , Brettanomyces bruxellensis , B. custersianus , Decera Anomala ( Dekkera anomala ), Streptomyces olivochromogenes ( Streptomyces olivochromogenes ), or Streptomyces griseus ( S. griseus ). The lactic acid bacteria include, for example, Bifidobacterium sp., Lactobacillus sp., Lactococcus sp., Pediococcus sp., and Streptococcus genus. ( Streptococcus. sp), or Leuconostoc sp. The fungi used in the fermentation may include, but are not limited to, mushrooms, such as shiitake mushrooms, oyster mushrooms, enoki mushrooms, pine mushrooms, button mushrooms, wood ear mushrooms, oyster mushrooms, and reishi mushrooms. , or it could be a situational mushroom.

The term "extract" used in this specification refers to a substance obtained by extracting components of a natural product, regardless of the extraction method, extraction solvent, extracted component, or form of the extract. It can include all substances that can be obtained by processing or processing in other ways. The extract can be obtained using extraction methods and extraction solvents known in the art. The extraction solvent is water, methanol, ethanol, isopropanol, butanol, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloromethane, N, N-dimethylformamide (DMF), and dimethyl sulfoxide (DMSO). , 1,3-butylene glycol, propylene glycol, or a mixed solvent thereof. Depending on the extraction solvent used, the degree of extraction and loss of the active ingredients of the plant extract may vary, so alcohol such as methanol, ethanol, isopropanol, and butanol is preferably used, and more preferably 70 to 100%. Ethanol is used as a solvent. The extraction method is not particularly limited, and for example, cold extraction, ultrasonic extraction, reflux cooling extraction, etc. can be used, and extraction can be performed at room temperature or by heating under conditions in which the active ingredients of the extraction mixture are not destroyed or minimized. In addition, the extract can be obtained through a conventional purification process in addition to extraction using the extraction solvent. For example, through various purification processes, such as ultrafiltration with a certain molecular weight cut-off value, cryofiltration, centrifugation, and separation by various chromatographies (chromatography according to size, charge, hydrophobicity, or affinity), etc. It can also be obtained through the obtained fraction, but is not limited to this.

The term “antioxidant” used herein refers to an action that inhibits, improves, or controls the destruction or damage of skin cells, biolipids, biomembrane phospholipids, proteins, DNA, etc. caused by active oxygen.

The term “anti-inflammatory” used herein refers to the inhibition of inflammation formed by the invasion of external infectious agents (bacteria, mold, viruses, various types of allergy-causing substances), delaying the onset of inflammation, improving the inflammatory state, or inflammatory state. It refers to any action that changes something beneficially.

As used herein, the term “improvement” means (a) inhibition of the development (b) alleviation and (c) elimination of skin inflammation in a subject, and includes the meaning of “prevention”. The term “prevention” refers to suppressing the occurrence of a disease or disease in an individual who has not been diagnosed as having the disease or disease but is prone to the disease or disease.

The term "individual" used herein refers to monkeys, cows, horses, pigs, sheep, dogs, cats, rats, mice, chimpanzees, etc., including humans, whose symptoms can be improved by administering the composition of the present invention. means mammals.

According to the first implementation example,

The present invention provides an antioxidant or anti-inflammatory agent comprising fermented extracts of Moringa oleifera (Leaf), Acorus calamus var. angustatus BESS. ( plant), and Lycium chinense MILL. ( Fruit) as active ingredients. Provides a cosmetic composition for use.

In the cosmetic composition according to the present invention, the weight ratio of Moringa oleifera (Leaf), Acorus calamus var. angustatus BESS., plant, and Lycium chinense MILL., Fruit is 1:1. :1.

In the cosmetic composition of the present invention, the fermentation extract will be contained in an amount of 0.5 to 20% by weight, preferably 1 to 10% by weight, and more preferably 2 to 6% by weight, based on the total weight of the cosmetic composition. You can. If the amount is less than 0.5% by weight, it is difficult to obtain the desired antioxidant and anti-inflammatory effects, and if it exceeds 20% by weight, there is no clear increase in effect as the content increases, resulting in low production economics.

In addition to the fermented extract, the cosmetic composition according to the present invention may additionally contain other ingredients that can have a synergistic effect on the effect of the fermented extract, preferably within a range that does not impair the effect aimed at by the present invention. there is. For example, the cosmetic composition of the present invention may contain conventional auxiliaries such as stabilizers, solubilizers, vitamins, pigments and fragrances, or carriers, as well as any other substances known to have antioxidant and anti-inflammatory effects. .

The cosmetic composition according to the present invention is not particularly limited in its formulation and can be prepared in any formulation commonly manufactured in the art, for example, solution, suspension, emulsion, paste, gel, cream, It can be formulated as lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray. On the other hand, scalp treatments, soaps, hair tonics, shampoos, rinses, hair packs, hair gels, lotions, conditioners, hair oils, mousses, creams, solids, solutions, emulsions, dispersants, micelles, liposomes, ointments, It may also be manufactured in the form of lotion, essence, patch, or spray.

When the formulation of the cosmetic composition is paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide are used as carrier ingredients. In the case of powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as carrier ingredients, and especially in the case of spray, chlorofluorohydrocarbon, propane/ It may contain a propellant such as butane or dimethyl ether.

When the formulation of the cosmetic composition is a solution or emulsion, a solvent, solubilizing agent, or emulsifying agent may be used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, and benzyl benzoate. , propylene glycol, 1,3-butyl glycol oil, glycerol fatty esters, polyethylene glycol, or fatty acid esters of sorbitan.

When the formulation of the cosmetic composition is a suspension, the carrier ingredients include water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, and miso. Crystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant can be used, and in the case of cleansing containing surfactants, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, and isethionate are used as carrier ingredients. , imidazolinium derivatives, methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester, etc. It can be used.

According to a specific embodiment of the present invention, fermentation comprising Moringa oleifera (Leaf), Acorus calamus var. angustatus BESS., plant, and Lycium chinense MILL., Fruit of the present invention The extract has excellent biocompatibility and not only exhibits radical scavenging activity but also has NO, TNF-a, IL-6, and COX-2 inhibitory effects. Therefore, the fermented extract of the present invention can be usefully used as an active ingredient in cosmetic compositions for antioxidant and anti-inflammatory purposes.

According to the second implementation,

The present invention provides an antioxidant or anti-inflammatory agent comprising fermented extracts of Moringa oleifera (Leaf), Acorus calamus var. angustatus BESS. ( plant), and Lycium chinense MILL. ( Fruit) as active ingredients. Provided is a pharmaceutical composition for use.

In the pharmaceutical composition according to the present invention, the weight ratio of Moringa oleifera (Leaf), Acorus calamus var. angustatus BESS., plant, and Lycium chinense MILL., Fruit is 1: It is characterized as 1:1.

The pharmaceutical composition according to the present invention comprises the extraction mixture in an amount of 0.0001 to 50% by weight, preferably 0.001 to 30% by weight, more preferably 0.01 to 20% by weight, based on the total weight of the pharmaceutical composition. do. If the amount is less than 0.0001% by weight, it is difficult to obtain the desired antioxidant and anti-inflammatory effects, and if it exceeds 50% by weight, there is no clear increase in effect as the content increases, resulting in low production economics.

The pharmaceutical composition according to the present invention includes dermal, oral, intravenous, intramuscular, intraarterial, transdermal, subcutaneous, intraperitoneal, intranasal, enteral, topical, sublingual or rectal administration, and oral or parenteral administration is preferred. However, it is not limited to this. The parenteral administration includes subcutaneous, intradermal, intravenous, intramuscular, and intralesional injection or infusion techniques.

The pharmaceutical composition according to the present invention may contain one or more active ingredients that exhibit the same or similar functions in addition to the fermented extract according to the present invention. The composition can be formulated and used in the form of oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, external preparations, suppositories, and sterile injection solutions according to conventional methods.

Solid preparations for oral administration of the pharmaceutical composition include powders, granules, tablets, capsules, soft capsules, pills, etc. Liquid preparations for oral use include suspensions, oral solutions, emulsions, syrups, etc. In addition to the commonly used simple diluents such as water and liquid paraffin, various excipients such as wetting agents, sweeteners, fragrances, and preservatives may be included. . Preparations for parenteral administration include external preparations such as powders, granules, tablets, capsules, sterilized aqueous solutions, solutions, non-aqueous solvents, suspensions, emulsions, syrups, suppositories, aerosols, etc. and sterilized injection preparations according to conventional methods. It can be formulated and used in the form of a cream, gel, patch, spray, ointment, warning agent, lotion, liniment agent, pasta agent, or cataplasma agent. It can be prepared and used as a pharmaceutical composition for external use on the skin. , but is not limited to this. Non-aqueous solvents and suspensions may include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate. As a base for suppositories, witepsol, macrogol, tween 61, cacao, laurin, glycerogeratin, etc. can be used.

The pharmaceutical composition according to the present invention may further contain preservatives, stabilizers, hydrating agents or emulsification accelerators, auxiliaries such as salts and/or buffering agents for adjusting osmotic pressure, and other therapeutically useful substances, and may be prepared by conventional methods. It can be formulated according to mixing, granulating or coating methods.

The pharmaceutical composition according to the present invention may vary depending on various factors, including the subject's age, weight, general health, gender, administration time, administration route, excretion rate, drug combination, and severity of a specific disease.

The pharmaceutical composition according to the present invention can be used alone or in combination with surgery, radiation therapy, hormone therapy, chemotherapy, and methods using biological response regulators.

According to the third implementation example,

The present invention provides a cosmetically or pharmaceutically effective amount of Moringa oleifera , Leaf, Acorus calamus var. angustatus BESS., plant, and Lycium chinense MILL., Fruit. Provided is a skin antioxidant and skin anti-inflammatory method comprising treating or administering a fermented extract to a subject.

In the method according to the present invention, the cosmetically or pharmaceutically effective amount is 0.0001 to 100 mg/kg, preferably 0.001 to 10 mg/kg, but is not limited thereto. Treatment or dosage can be adjusted depending on the specific patient's weight, age, gender, health condition, diet, administration period, administration method, clearance rate, disease severity, etc. Treatment or administration may be administered once a day or may be divided into several times.

In the method according to the present invention, the subject is a vertebrate, preferably a mammal, more preferably a human or laboratory animal such as a rat, rabbit, guinea pig, hamster, dog, or cat, and most preferably a human. It may be, but is not limited to this.

In the method according to the present invention, the treatment or administration method can be applied, orally or parenterally, and when administered parenterally, intraperitoneal injection, intrarectal injection, subcutaneous injection, intravenous injection, intramuscular injection, intrauterine dura. It may be administered by injection, intracerebrovascular injection, or intrathoracic injection.

Hereinafter, the configuration and effects of the present specification will be described in more detail through examples, test examples, and production examples. However, these examples, test examples, and manufacturing examples are provided only for illustrative purposes to aid understanding of the present specification, and the scope and scope of the present specification are not limited by the examples below.

<Manufacturing example>

Preparation Example 1. Moringa leaves ( Moringa oleifera , Leaf) Preparation of extract

300 g of dried Moringa leaves were extracted with 40 times the volume of tertiary distilled water for 2 hours (85 ± 2 °C, 1.2 Pa) and then concentrated to 50%. The extract was inoculated with yeast ( Saccharomyces cerevisiae ) at a concentration of 5 × 10 ⁶ CFU/mL, co-cultured at 37 ± 1°C for 24 hours, and then sterilized (30 minutes at 120°C). Next, the resulting culture was filtered through 4 layers of gauze, centrifuged (6,000 G/10 min), the supernatant was taken, freeze-dried, and the resulting dried powder was stored frozen (-20°C) for use.

Preparation Example 2. Calamus Outpost ( Acorus calamus var. angustatus BESS., Preparation of plant extract

Iris alla extract was prepared using the same method as Preparation Example 1, except that dried irises alla was used instead of dried moringa leaves.

Preparation Example 3. Goji berry fruit ( Lycium chinense MILL., Manufacture of fruit extract

Goji berry extract was prepared using the same method as Preparation Example 1, except that dried goji berry fruit was used instead of dried moringa leaves.

Preparation Examples 4 to 7. Preparation of mixed extract of moringa leaves, calamus whole plant, and goji berry fruit

A mixed extract of Moringa leaves, iris whole plant, and Goji berry fruit was prepared using the same method as Preparation Example 1, except that the mixed extract (unit: g) according to Table 1 below was used instead of the dried Moringa leaves. did.

No. moringa leaves Iris Outpost goji berries Production example 4 150 150 - Production example 5 - 150 150 Production example 6 150 - 150 Production example 7 100 100 100

<Experimental example>

Experimental Example 1. Antioxidant effect

To confirm the antioxidant activity of the extracts prepared in Preparation Examples 1 to 7, a DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging assay was performed. DPPH reagent was prepared by dissolving it in methanol to 0.5mM, and each of the extracts according to Preparation Examples 1 to 7 above was diluted in DPBS and placed in 100ul of a 96-well plate. 100ul of the prepared DPPH reagent was added and reacted in the dark for 30 minutes. I ordered it. It was analyzed at a wavelength of 517 nm using a microplate reader (SpectraMax ABS, Molecular Devices Co.) and compared with Ascrobic acid, a positive control.

As a result, Preparation Examples 4 to 5, which are mixed extracts of moringa leaves, iris sprouts, and goji berry fruits according to the present invention, were found to have superior free radical scavenging rate (%) compared to the individual extracts of moringa leaves, iris sprouts, and goji berry fruits. In particular, Preparation Example 7 containing moringa leaves, iris sprouts, and goji berry fruits at a weight ratio of 1:1:1 was found to have excellent antioxidant activity, almost similar to that of ascorbic acid (Figure One).

Experimental Example 2. Anti-inflammatory effect

In order to determine the anti-inflammatory effect of the extracts prepared in Preparation Examples 1 to 7, an inflammatory cytokine production inhibition activity analysis was performed. First, Raw 264.7 was inoculated into a 96-well plate with DMEM medium containing 10% FBS (fetal bovine serum) at 4×10 ⁴ per well, and then cultured in an incubator at 5% CO ₂ and 37°C for 24 hours. After culturing, the medium was removed, and the extracts according to Preparation Examples 1 to 7 were dissolved in DPBS at 100 mg/ml, diluted with DMEM medium, and cultured for 24 hours. The cultured 96-well plate was centrifuged to collect other suspended matter in the culture medium at the bottom of the plate and harvest the clean culture medium. Next, the anti-inflammatory efficacy was evaluated using the harvested culture fluid using NO assay and ELISA kit for TNF-a, IL-6, and COX-2.

As a result, Preparation Examples 4 to 5, which are mixed extracts of moringa leaves, iris sprouts, and goji berry fruits according to the present invention, have NO, TNF-a, IL-6, and It was shown to have excellent COX-2 inhibitory activity. In particular, Preparation Example 7 containing moringa leaves, iris sprouts, and goji berry fruits at a weight ratio of 1:1:1 showed a similar effect to the LPS-stimulated untreated group by inhibiting NO activity by 93.66%, and TNF at 61.79%. -a inhibition rate, IL-6 inhibition rate of 99.56%, and COX-2 inhibition rate of 38.64%, demonstrating that it has an excellent anti-inflammatory effect by inhibiting the production of various inflammatory cytokines (Figures 2 to 5).

Experimental Example 3. Cytotoxicity

To determine the cytotoxicity of the fermented extract prepared in Preparation Example 7, Raw264.7 cytotoxicity and HDP cytotoxicity experiments were performed. Raw 264.7, a mouse macrophage cell line, was inoculated into a 96-well plate with DMEM medium containing 10% FBS (fetal bovine serum) at 4×10 ⁴ cells per well, and then cultured in an incubator at 5% CO ₂ at 37°C for 24 hours. did. In addition, human hair follicle dermal papilla cells (HDP), which are human hair papilloma cells, were inoculated into a 96-well plate at 2×10 ⁴ cells per well with DMEM medium containing 10% FBS (fetal bovine serum), and then incubated with 5% FBS (fetal bovine serum). CO ₂ , cultured in a 37°C incubator for 24 hours. After culturing, each medium was removed, and the fermented extract according to Preparation Example 7 was dissolved in DPBS at 100 mg/ml, diluted using DMEM medium, and cultured for 24 hours. After incubation, MTT (3-(4,5-Dimethylthiazol-2-yl_-2,5,-Diphenyltetrazolium Bromide) reagent was dissolved in Serum Free DMEM medium, incubated for 1 hour and 30 minutes, and incubated with a Microplate reader (SpectraMax ABS, Molecular Devices Co.). ) Cytotoxicity was confirmed by comparing the sample to the untreated control group by analyzing it at a wavelength of 570 nm using equipment.

As a result, Preparation Example 7 according to the present invention, especially containing moringa leaves, calamus whole plant, and goji berry fruit at a weight ratio of 1:1:1, showed excellent cell survival rate (FIG. 6).

We looked at the examples mainly. A person skilled in the art to which the present invention pertains will understand that the present invention may be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered from an illustrative rather than a restrictive perspective. The scope of the present invention is indicated in the claims rather than the foregoing description, and all differences within the equivalent scope should be construed as being included in the present invention.

Claims (7)

Antioxidant or anti-inflammatory cosmetic composition containing fermented extracts of Moringa oleifera (Leaf), Acorus calamus var. angustatus BESS., plant, and Lycium chinense MILL., Fruit as active ingredients .
According to paragraph 1,
The fermented extract is an antioxidant or anti-inflammatory cosmetic composition, characterized in that it is obtained by inoculating yeast in a medium containing dried moringa leaves, iris apricots, and goji berry fruits and then culturing them.
According to paragraph 2,
An antioxidant or anti-inflammatory cosmetic composition, wherein the yeast is Saccharomyces cerevisiae.
According to paragraph 2,
An antioxidant or anti-inflammatory cosmetic composition, wherein the culturing is performed at a temperature of 20°C to 40°C for 12 to 60 hours.
According to paragraph 1,
The weight ratio of the Moringa oleifera (Leaf), Acorus calamus var. angustatus BESS., plant, and Lycium chinense MILL., Fruit is 1:1:1. , Antioxidant or anti-inflammatory cosmetic composition.
According to paragraph 1,
An antioxidant or anti-inflammatory cosmetic composition, characterized in that the fermented extract is contained in a concentration of 0.001 to 20 mM in the cosmetic composition.
According to paragraph 1,
The formulation of the cosmetic composition includes lotion (skin lotion), skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutritional lotion, massage cream, nutritional cream, eye cream, moisture cream, hand cream, foundation, essence, An antioxidant or anti-inflammatory cosmetic composition characterized by one or more selected from the group consisting of nutritional essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.