KR102552650B1 - Abalone Cordyceps and manufacturing method thereof - Google Patents

Abstract

The present invention is to grow Cordyceps sinensis by inoculating pretreated abalone with a spore suspension of the Cordyceps sinensis strain. According to the method of producing Cordyceps sinensis using abalone of the present invention, the abalone is blanched and then sterilized and the abalone medium is inoculated with a spore suspension of the Cordyceps sinensis strain to cultivate the Cordyceps sinensis strain in the abalone. When the Cordyceps sinensis strain is used to inoculate abalone and culture the same, the Cordyceps sinensis strain is cultured for 40 to 45 days at a temperature of 20 to 28℃ to grow Cordyceps sinensis. At this time, the culturing process comprises: a first step, which is a pre-culture, of culturing for 5 to 10 days at a temperature of 23 to 28℃; and a second step, which is a post-culture, of culturing for 30 to 35 days at a temperature of 20 to 22℃ after the first stage of pre-culture. The present invention can use the nutritional source of abalone to further increase the pharmacological effect of Cordyceps sinensis, thereby greatly increasing the demand for Cordyceps sinensis. In particular, the Cordyceps sinensis of the present invention contains a large amount of polysaccharides and phenolic ingredients, including beta-glucan, as well as cordycepin, a specific ingredient. Thus, immune-boosting and antibacterial effects can be obtained when the Cordyceps sinensis is added and used as food or other raw materials.

Description

Cordyceps sinensis and its cultivation method using abalone {Abalone Cordyceps and manufacturing method thereof}

The present invention relates to a method for producing Cordyceps sinensis using abalone, and more particularly, to growing Cordyceps sinensis by inoculating a spore suspension of a Cordyceps sinensis strain into pretreated abalone.

. Abalone, which is widely farmed in the Wando region of Korea, is rich in vitamins and minerals and is used in various ways as a raw material for food. The long oval shell of abalone has a thin, bumpy surface, and has 3 to 4 absorption holes through which water passes. When mature, the ovaries of females are dark green, and the testes of males are pale yellow or milky white.

Abalone has a high water content and low protein and fat content, and is well absorbed by the body, so it is often used as a healthy food for convalescent patients or the elderly. The innards of abalone are called crabs and are sometimes eaten with salted fish. However, during the spawning season, it is toxic to the intestines, so it is better to avoid raw food and eat it slightly cooked.

In this way, abalone has ingredients such as glutamic acid and glycine, so it has a savory and sweet taste, and it is recommended as a healthy food for middle-aged or older because it has much less fat and more protein than other fish. In addition, abalone contains 1100 mg of an amino acid called arginine, which is much more than other foods, and since the arginine is known to be deeply related to the expression of sexual power, its value can be said to be sufficiently meaningful.

Summarizing the efficacy of abalone as described above, if you boil abalone porridge when you are weak, you will have energy, make urine come out well, help with jaundice or cystitis, relieve symptoms of burning throat or chest pain, and strengthen the liver. there is In addition, the symptoms of headache, ringing in the ears, and dry tongue and throat due to excessive activity of the liver function are called liver yang.

Cordyceps militaris is a medicinal mushroom belonging to the family Clavicipitaceae, and the name Cordyceps is derived from its growth characteristics. It is used as a very important medicinal material in Asia, including Korea and China, and various attempts are being made to secure it. Cordyceps contains various active ingredients including cordycepin, and various pharmacological activities such as anticancer, antidiabetic, metabolic disease improvement, and antiviral activity have been reported.

Recently, Cordyceps sinensis is not only used as a medicinal raw material, but also used to provide nutrients and functional effects of food, and is further added to livestock feed to effectively provide livestock health.

In the meantime, various types of cordyceps that grow on the dead bodies of insects have been developed, but there is no cordyceps using abalone yet. In particular, abalone is rich in various nutrients as described above, and nothing has been known about the effect of this abalone when combined with Cordyceps sinensis. solved the problem.

Korean Patent Publication No. 2010-0061780 (2010. 06. 09)

An object of the present invention is to use abalone as a medium to increase the pharmacological effect of Cordyceps sinensis in order to solve the above problems. The purpose of cultivating abalone Cordyceps sinensis as described above is to increase the demand for abalone and to provide for use in the development of various foods by combining the nutritional source of abalone with the pharmacological effects of Cordyceps sinensis.

In addition, the purpose of the Cordyceps sinensis of the present invention is to cultivate Cordyceps sinensis containing a large amount of polysaccharides including beta-glucan and phenolic components as well as the specific component cordycepin.

When Cordyceps sinensis containing a large amount of the above components is used as food or other raw materials, it provides immunity enhancement and antibacterial effects.

In addition, when used as a feed additive for livestock using Cordyceps sinensis as described above, it promotes growth and improves quality.

In the method for producing Cordyceps sinensis using abalone of the present invention, the pretreated abalone is blanched and sterilized, and the spore suspension of the Cordyceps strain is inoculated therein to cultivate the Cordyceps sinensis strain in the abalone.

The Cordyceps sinensis strain is subcultured on PDA agar medium, and the Cordyceps sinensis strain subcultured on the solid medium is cultured in an incubator at 22-27 ° C for 20-25 days and used as an inoculum strain of liquid medium.

When the Cordyceps sinensis strain is inoculated into abalone and cultured, it is cultivated for 40 to 45 days at a temperature of 20 to 28 ° C. to grow Cordyceps sinensis.

At this time, the pre-cultivation step of culturing the culture step at a temperature of 23-28 ° C. for 5-10 days,

It is preferable to configure a second step of culturing after culturing for 30 to 35 days at a temperature of 20 to 22 ° C. after the first step of the pre-cultivation.

Cordyceps sinensis using abalone of the present invention greatly increases the demand for abalone, thereby increasing income from abalone farming and at the same time providing the effect of enabling it to be used as a raw material for various foods.

In addition, by inoculating and culturing abalone with a Cordyceps sinensis strain, there is an effect of enabling abalone to be consumed in various ways.

In addition, in order to further increase the pharmacological effect of Cordyceps sinensis, the nutritional source of abalone can be used, which provides an effect of greatly increasing the demand for Cordyceps sinensis.

In particular, Cordyceps sinensis of the present invention contains a large amount of polysaccharides and phenolic components including beta-glucan as well as cordycepin, which is a specific component, and provides immunity enhancement and antibacterial effects when used as food or other raw materials by adding them.

The present invention relates to Cordyceps sinensis using abalone and a method for culturing the same, and will be described in detail using examples.

First, when abalone is sold as a normal product, it grows to a size of about 100g after 2-3 years of farming. However, in the present invention, the size of the abalone is not important, so the cordyceps strain is cultured using abalone of an appropriate size.

First, the washed abalone is blanched for 10 to 30 seconds, and then the intestines are removed. Then, if the size is small, it is possible to culture the strain of Cordyceps sinensis as it is, but preferably, the size is divided to a certain extent to inoculate the strain of Cordyceps sinensis. At this time, it is preferable to segment in a size of 1 to 10 mm.

In the method for producing Cordyceps sinensis using abalone of the present invention, the pretreated abalone is blanched and sterilized, and the spore suspension in which the Cordyceps strain is cultured in a liquid medium is inoculated therein to cultivate the Cordyceps sinensis strain in the abalone. The temperature for boiling abalone is preferably 80 to 105 ° C.

In the sterilization process, sterilization is performed for 30 to 60 minutes at a temperature of 120 to 125 ° C and a pressure of 1 to 1.3, and then the spore suspension is inoculated. At the time of inoculation, the spore suspension is injected or sprayed to inoculate the strain of Cordyceps sinensis.

If not sterilized, the growth of pathogens or other microorganisms rather than the growth of Cordyceps strains may be contaminated.

First, the Cordyceps sinensis strain is subcultured on PDA agar medium, which is a solid medium, and the Cordyceps sinensis strain subcultured on the solid medium is cultured in an incubator at 22 to 27 ° C for 20 to 25 days and used as an inoculum strain of liquid medium. As described above, the Cordyceps sinensis strain is subcultured so that it can be used continuously to maintain the characteristics of the strain uniformly.

The liquid medium is prepared by mixing 0.2 to 0.7 parts by weight of yeast extract, 0.8 to 1.5 parts by weight of tryptone, and 0.8 to 1.5 parts by weight of sodium chloride in 100 parts by weight of water to make a medium solution, and dextrose 1 to the medium solution. Mix ~ 3 parts by weight to prepare a liquid medium.

A Cordyceps spore suspension is prepared by mixing the subcultured Cordyceps strains in the liquid medium. The Cordyceps spore suspension is mixed with 0.1 to 1 part by weight of Cordyceps sinensis strain in 100 parts by weight of liquid medium and inoculated into pretreated abalone to culture Cordyceps sinensis.

When inoculating abalone with a spore suspension, the spore suspension is inoculated by injection or spraying, and it is preferable to inoculate at a rate of 0.05 to 0.5 parts by weight of the spore suspension per 100 parts by weight of the abalone.

Cordyceps strains inoculated into the abalone medium are inoculated with a mixture of Paecilomyces japonica , Cordyceps militaris , and Cordyceps sinensis , or inoculated separately to cultivate Cordyceps sinensis.

In the case of mixed inoculation, inoculate a Cordyceps strain mixed with Paecilomyces japonica, Cordyceps militaris , and Cordyceps sinensis at a ratio of 2: 5: 2, and then culture By optimizing the temperature and period, the content of the cordycepin component in Cordyceps sinensis is increased.

The cordyceps strains, that is, Paecilomyces japonica , Cordyceps militaris , and Cordyceps sinensis were subcultured on PDA agar medium, respectively, and subcultured on the agar medium Each strain of Cordyceps sinensis is cultured in an incubator at 22-28 ° C for around 25 days and used as an inoculum strain of liquid medium. As described above, each strain of Cordyceps sinensis is subcultured so that it can be continuously used to maintain the characteristics of the strain uniformly.

Cordyceps strains each cultured in the liquid medium were mixed with Paecilomyces japonica , Cordyceps militaris , and Cordyceps sinensis at a ratio of 2: 5: 2 to obtain Cordyceps sinensis Produce mixed strains.

The Cordyceps sinensis mixture is inoculated into an abalone medium to culture Cordyceps sinensis. At this time, the inoculation is preferably inoculated at a ratio of 0.1 to 1 part by weight of the liquid medium mixed strain to 100 parts by weight of the abalone medium.

When the spore suspension using the Cordyceps strain is inoculated into abalone and cultured, it is cultured for 40 to 45 days at a temperature of 20 to 28 ° C. to grow Cordyceps sinensis.

If the incubation temperature is low, the growth of Cordyceps sinensis is delayed, which may result in high costs and poor growth. In addition, at a temperature higher than 28 ° C., the growth of the Cordyceps sinensis strain is poor due to the overgrowth of the Cordyceps sinensis strain or the drying of the abalone medium. When growing Cordyceps sinensis strains in abalone medium, it is preferable to maintain a relative humidity of 92 to 97%.

When culturing the Cordyceps sinensis strain, culturing in two stages under different temperature conditions leads to fast growth of Cordyceps sinensis, and the mycelium of Cordyceps sinensis grows healthily. That is, the pre-cultivation step of culturing the first step at a temperature of 23 to 28 ° C. for 5 to 10 days, and the post-cultivation step of culturing the first step of the pre-cultivation at a temperature of 20 to 22 ° C. for 30 to 35 days. It is preferable to do it in two stages consisting of.

Example

① After washing the harvested abalone, blanch it in boiling water at 100℃ for 25 seconds and remove the intestines.

② The abalone with the intestines removed is sterilized at 121°C and 1.2 atm for 60 minutes.

③ Prepare a spore suspension by mixing 0.5 parts by weight of Cordyceps sinensis strain with 100 parts by weight of water.

④ When inoculating abalone with spore suspension, inoculate 100 parts by weight of abalone with 0.1 part by weight of spore suspension.

⑤ In the culturing step, that is, culturing for 7 days at a temperature of 25 ° C in the first step, and then culturing for 33 days at a temperature of 20 ° C after the first step culture to grow Cordyceps sinensis.

Cordyceps sinensis of the present invention has a high content of beta-glucan and cordycepin, beta-glucan is effective in improving immunity, and cordycepin component has an antibacterial effect, so it is used as a natural antibiotic.

Natural products, including Cordyceps sinensis, vary greatly in their components and efficacy depending on the growing environment.

Cordyceps sinensis (冬蟲夏草) is a high value-added biological material that has been recognized for its biological material value since ancient times. After incubating in the body of insects in winter, absorbing nutrients and killing insects, cordyceps develops in a hot and humid environment in summer, and has been known as one of the three great medicines along with ginseng and antler.

Cordyceps sinensis is originally a medicinal mushroom derived from insects. When cultivated using insects, it is possible to produce enough useful substances using abundant protein sources, and it is a material that can further improve insects, and pharmacological effects can also be expected. It is very useful for food.

In relation to enoki mushrooms, which have recently caused deaths due to food poisoning in the United States, bottle cultivation of cordyceps can be safely grown from bacteria such as food poisoning bacteria because it is grown inside the bottle, unlike other bottle-grown mushrooms such as enoki and shiitake.

Cordycepin substances have various pharmacological properties, such as anti-inflammatory, antioxidant and anti-cancer, adult diseases such as diabetes and hypertension, as well as immune activation functions in vitro and in vivo .

Cordycepin, a representative indicator substance of Cordyceps sinensis, can only be obtained from Cordyceps sinensis for food use, and is a functional substance with various pharmacological effects (anti-cancer and antioxidant, immunity, various adult diseases, etc.). It is also possible to use as a feed additive by using.

Claims (5)

In the method for manufacturing Cordyceps sinensis using abalone,
Cordyceps sinensis using the abalone
After boiling the washed abalone at a temperature of 80 to 105℃ for 10 to 30 seconds, remove the intestines, cut into 1 to 10 mm pieces, and sterilize at a temperature of 120 to 125 ℃ and a pressure of 1 to 1.3 for 30 to 60 minutes. step,
Preparing a Cordyceps sinensis strain to be inoculated into a liquid medium by culturing the Cordyceps sinensis strain subcultured in a PDA agar medium, which is a solid medium, in an incubator at a temperature of 22 to 27 ° C for 20 to 25 days,
0.2 to 0.7 parts by weight of yeast extract, 0.8 to 1.5 parts by weight of tryptone, and 0.8 to 1.5 parts by weight of sodium chloride are mixed with 100 parts by weight of water to make a medium solution, and 1 to 3 parts by weight of dextrose is mixed with the medium solution to prepare a liquid medium steps to do,
A cordyceps spore suspension is prepared by mixing 0.1 to 1 part by weight of a cordyceps strain with 100 parts by weight of the liquid medium, but the cordyceps strains are Paecilomyces japonica , Cordyceps militaris , Cordyceps sinen Cis ( Cordyceps sinensis ) strain is used,
Inoculating 100 parts by weight of the sterilized abalone at a rate of 0.05 to 0.5 parts by weight of the cordyceps spore suspension;
Method for producing cordyceps using abalone, characterized in that the spore suspension using the cordyceps strain is incubated in abalone and cultured at a temperature of 20 to 28 ° C and a humidity of 92 to 97% for 40 to 45 days
delete According to claim 1,
Cordyceps strains each cultured in the liquid medium are Paecilomyces japonica ( Paecilomyces japonica ), Cordyceps militaris ( Cordyceps militaris ), Cordyceps sinensis ( Cordyceps sinensis ) Mixed at a ratio of 2: 5: 2 Method for producing Cordyceps sinensis using abalone, characterized in that the strain is inoculated and cultured in abalone
delete According to claim 1,
When the Cordyceps sinensis strain is inoculated into abalone and cultured, the pre-cultivation step of culturing for 5 to 10 days at a temperature of 23 to 28 ° C in step 1,
Method for producing Cordyceps sinensis using abalone, characterized in that it consists of a post-cultivation step of culturing for 30-35 days at a temperature of 20-22 ° C after the pre-cultivation step.