KR102536221B1 - 단백질 생산을 위한 지질 대사의 조정 - Google Patents

단백질 생산을 위한 지질 대사의 조정 Download PDF

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KR102536221B1
KR102536221B1 KR1020187034453A KR20187034453A KR102536221B1 KR 102536221 B1 KR102536221 B1 KR 102536221B1 KR 1020187034453 A KR1020187034453 A KR 1020187034453A KR 20187034453 A KR20187034453 A KR 20187034453A KR 102536221 B1 KR102536221 B1 KR 102536221B1
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ala
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cell
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KR20190003651A (ko
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제임스 버지
크리스토퍼 마크 스메일
타냐 나이트
로버트 영
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론자 리미티드
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0097—Oxidoreductases (1.) acting on reduced flavodoxin as donor (1.19)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/19—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)
    • C12Y114/19001—Stearoyl-CoA 9-desaturase (1.14.19.1), i.e. DELTA9-desaturase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Reproductive Health (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
KR1020187034453A 2016-05-03 2017-05-03 단백질 생산을 위한 지질 대사의 조정 Active KR102536221B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662330973P 2016-05-03 2016-05-03
US62/330,973 2016-05-03
PCT/EP2017/060484 WO2017191165A1 (en) 2016-05-03 2017-05-03 Modulation of lipid metabolism for protein production

Publications (2)

Publication Number Publication Date
KR20190003651A KR20190003651A (ko) 2019-01-09
KR102536221B1 true KR102536221B1 (ko) 2023-05-23

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US (3) US10655111B2 (enExample)
EP (1) EP3452608B1 (enExample)
JP (1) JP6959942B2 (enExample)
KR (1) KR102536221B1 (enExample)
CN (1) CN109415748A (enExample)
AU (1) AU2017259010B2 (enExample)
CA (1) CA3023038C (enExample)
EA (1) EA201892503A1 (enExample)
ES (1) ES2938626T3 (enExample)
IL (2) IL262646B (enExample)
MX (1) MX2018013499A (enExample)
SG (1) SG11201809626PA (enExample)
WO (1) WO2017191165A1 (enExample)

Families Citing this family (9)

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CA3023038C (en) * 2016-05-03 2023-03-14 Lonza Ltd Modulation of lipid metabolism for protein production
WO2020014483A1 (en) * 2018-07-11 2020-01-16 Cornell University Supported plant plasma membrane lipid bilayer on-a-chip
EA202092684A1 (ru) 2018-08-27 2021-03-11 Ридженерон Фармасьютикалз, Инк. Применение рамановской спектроскопии для последующей очистки
US12331320B2 (en) 2018-10-10 2025-06-17 The Research Foundation For The State University Of New York Genome edited cancer cell vaccines
US20230193341A1 (en) * 2018-10-24 2023-06-22 Selexis Sa Expression systems, recombinant cells and uses thereof
EP3942048A1 (en) 2019-03-20 2022-01-26 Regeneron Pharmaceuticals, Inc. Treatment of increased lipid levels with sterol regulatory element binding transcription factor 1 (srebf1) inhibitors
CN110317799A (zh) * 2019-07-18 2019-10-11 江南大学 一种人源岩藻糖转移酶8的原核表达方法及其产品
US20220403398A1 (en) 2019-11-14 2022-12-22 Lonza Ltd Methods of cell selection
CN115029307A (zh) * 2022-07-28 2022-09-09 吉林大学 一种延缓MSCs衰老的方法

Citations (1)

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Publication number Priority date Publication date Assignee Title
WO2009062789A1 (en) 2007-11-13 2009-05-22 Boehringer Ingelheim Pharma Gmbh & Co Kg Improving the secretory capacity in host cells

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GB9022545D0 (en) 1990-10-17 1990-11-28 Wellcome Found Culture medium
EP1958962A3 (en) 1997-06-12 2013-05-01 Novartis International Pharmaceutical Ltd. Artificial antibody polypeptides
JP2003516124A (ja) 1999-10-15 2003-05-13 ユニバーシティー オブ マサチューセッツ 標的とした遺伝的干渉の手段としてのｒｎａ干渉経路遺伝子
US6326193B1 (en) 1999-11-05 2001-12-04 Cambria Biosciences, Llc Insect control agent
WO2001096584A2 (en) 2000-06-12 2001-12-20 Akkadix Corporation Materials and methods for the control of nematodes
WO2002095008A2 (en) 2001-02-02 2002-11-28 Bayer Corporation Methods of modulating surfactant phospholipid production
CA2528418A1 (en) 2003-06-11 2004-12-23 Biogen Idec Ma Inc. Method to increase protein production in culture
CN102286483B (zh) 2004-04-16 2014-06-04 中化帝斯曼制药有限公司荷兰公司 用于在真菌细胞中表达基因的真菌启动子
AU2006249087B2 (en) * 2005-05-20 2012-05-17 Lonza Biologics Plc High-level expression of recombinant antibody in a mammalian host cell
US20080182249A1 (en) * 2006-09-01 2008-07-31 Fox Brian G Expression systems for mammalian and mycobacterial desaturases
CA3015426C (en) * 2008-11-18 2020-01-14 Commonwealth Scientific And Industrial Research Organisation Recombinant cells comprising exogenous .delta.5 and .delta.6 desaturases, .delta.5 and .delta.6 elongases, and .delta.4 desaturases
PL2951309T3 (pl) * 2013-02-01 2019-06-28 Selexis S.A. Wzmocniona ekspresja transgenu i przetwarzanie
SG11201600872UA (en) * 2013-08-06 2016-03-30 Lonza Biologics Plc Means and methods for the generation of mammalian producer cells for the production of recombinant proteins
CA3023038C (en) 2016-05-03 2023-03-14 Lonza Ltd Modulation of lipid metabolism for protein production

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009062789A1 (en) 2007-11-13 2009-05-22 Boehringer Ingelheim Pharma Gmbh & Co Kg Improving the secretory capacity in host cells

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Cell, Vol. 87, 415-426, November 1, 1996
J. Dairy Sci. 99:783-795

Also Published As

Publication number Publication date
IL262646B (en) 2022-08-01
SG11201809626PA (en) 2018-11-29
US10655111B2 (en) 2020-05-19
WO2017191165A1 (en) 2017-11-09
ES2938626T3 (es) 2023-04-13
US20230047838A1 (en) 2023-02-16
IL294241B1 (en) 2023-06-01
EP3452608B1 (en) 2022-11-30
US20200231944A1 (en) 2020-07-23
KR20190003651A (ko) 2019-01-09
BR112018072434A2 (pt) 2019-02-19
US11434474B2 (en) 2022-09-06
AU2017259010B2 (en) 2021-04-22
JP2019514399A (ja) 2019-06-06
CA3023038A1 (en) 2017-11-09
US20170321194A1 (en) 2017-11-09
IL262646A (en) 2018-12-31
EP3452608A1 (en) 2019-03-13
MX2018013499A (es) 2019-09-11
IL294241B2 (en) 2023-10-01
IL294241A (en) 2022-08-01
CN109415748A (zh) 2019-03-01
AU2017259010A1 (en) 2018-11-22
EA201892503A1 (ru) 2019-09-30
CA3023038C (en) 2023-03-14
JP6959942B2 (ja) 2021-11-05

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