CA3023038C - Modulation of lipid metabolism for protein production - Google Patents
Modulation of lipid metabolism for protein production Download PDFInfo
- Publication number
- CA3023038C CA3023038C CA3023038A CA3023038A CA3023038C CA 3023038 C CA3023038 C CA 3023038C CA 3023038 A CA3023038 A CA 3023038A CA 3023038 A CA3023038 A CA 3023038A CA 3023038 C CA3023038 C CA 3023038C
- Authority
- CA
- Canada
- Prior art keywords
- cell
- lmm
- recombinant polypeptide
- nucleic acid
- fold
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0097—Oxidoreductases (1.) acting on reduced flavodoxin as donor (1.19)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4702—Regulators; Modulating activity
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
- C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
- C12N9/0083—Miscellaneous (1.14.99)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
- C12Y114/19—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)
- C12Y114/19001—Stearoyl-CoA 9-desaturase (1.14.19.1), i.e. DELTA9-desaturase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Reproductive Health (AREA)
- Cell Biology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662330973P | 2016-05-03 | 2016-05-03 | |
| US62/330,973 | 2016-05-03 | ||
| PCT/EP2017/060484 WO2017191165A1 (en) | 2016-05-03 | 2017-05-03 | Modulation of lipid metabolism for protein production |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA3023038A1 CA3023038A1 (en) | 2017-11-09 |
| CA3023038C true CA3023038C (en) | 2023-03-14 |
Family
ID=58745198
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3023038A Active CA3023038C (en) | 2016-05-03 | 2017-05-03 | Modulation of lipid metabolism for protein production |
Country Status (13)
| Country | Link |
|---|---|
| US (3) | US10655111B2 (enExample) |
| EP (1) | EP3452608B1 (enExample) |
| JP (1) | JP6959942B2 (enExample) |
| KR (1) | KR102536221B1 (enExample) |
| CN (1) | CN109415748A (enExample) |
| AU (1) | AU2017259010B2 (enExample) |
| CA (1) | CA3023038C (enExample) |
| EA (1) | EA201892503A1 (enExample) |
| ES (1) | ES2938626T3 (enExample) |
| IL (2) | IL262646B (enExample) |
| MX (1) | MX2018013499A (enExample) |
| SG (1) | SG11201809626PA (enExample) |
| WO (1) | WO2017191165A1 (enExample) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017191165A1 (en) | 2016-05-03 | 2017-11-09 | Lonza Ltd | Modulation of lipid metabolism for protein production |
| US12077786B2 (en) * | 2018-02-02 | 2024-09-03 | Lonza Ltd | Methods of cell selection and modifying cell metabolism |
| US20210324366A1 (en) * | 2018-07-11 | 2021-10-21 | Cornell University | Supported plant plasma membrane lipid bilayer on-a-chip |
| MY204234A (en) | 2018-08-27 | 2024-08-16 | Regeneron Pharma | Use of raman spectroscopy in downstream purification |
| US12331320B2 (en) | 2018-10-10 | 2025-06-17 | The Research Foundation For The State University Of New York | Genome edited cancer cell vaccines |
| US20230193341A1 (en) * | 2018-10-24 | 2023-06-22 | Selexis Sa | Expression systems, recombinant cells and uses thereof |
| JP7574206B2 (ja) | 2019-03-20 | 2024-10-28 | リジェネロン・ファーマシューティカルズ・インコーポレイテッド | ステロール調節エレメント結合転写因子１（ｓｒｅｂｆ１）阻害剤による上昇した脂質レベルの治療 |
| CN110317799B (zh) * | 2019-07-18 | 2026-04-03 | 江南大学 | 一种人源岩藻糖转移酶8的原核表达方法及其产品 |
| WO2021094461A1 (en) | 2019-11-14 | 2021-05-20 | Lonza Ltd | Methods of cell selection |
| CN115029307A (zh) * | 2022-07-28 | 2022-09-09 | 吉林大学 | 一种延缓MSCs衰老的方法 |
| WO2026062080A1 (en) | 2024-09-17 | 2026-03-26 | Ares Trading S.A. | Cell having increased secretion capabilities and method of use |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9022545D0 (en) | 1990-10-17 | 1990-11-28 | Wellcome Found | Culture medium |
| JP3614866B2 (ja) | 1997-06-12 | 2005-01-26 | リサーチ コーポレイション テクノロジーズ，インコーポレイティド | 人工抗体ポリペプチド |
| EP1235842A4 (en) | 1999-10-15 | 2003-04-23 | Univ Massachusetts | Rna interference pathway genes as tools for targeted genetic interference |
| US6326193B1 (en) | 1999-11-05 | 2001-12-04 | Cambria Biosciences, Llc | Insect control agent |
| WO2001096584A2 (en) | 2000-06-12 | 2001-12-20 | Akkadix Corporation | Materials and methods for the control of nematodes |
| WO2002095008A2 (en) * | 2001-02-02 | 2002-11-28 | Bayer Corporation | Methods of modulating surfactant phospholipid production |
| JP2007500016A (ja) * | 2003-06-11 | 2007-01-11 | バイオジェン・アイデック・エムエイ・インコーポレイテッド | 培養においてタンパク質生成を増大させるための方法 |
| CA2560205A1 (en) | 2004-04-16 | 2005-10-27 | Dsm Ip Assets B.V. | Fungal promoters for expressing a gene in a fungal cell |
| US20090215165A1 (en) * | 2005-05-20 | 2009-08-27 | James Rance | High-level expression of recombinant antibody in a mammalian host cell |
| US20080182249A1 (en) * | 2006-09-01 | 2008-07-31 | Fox Brian G | Expression systems for mammalian and mycobacterial desaturases |
| WO2009062789A1 (en) | 2007-11-13 | 2009-05-22 | Boehringer Ingelheim Pharma Gmbh & Co Kg | Improving the secretory capacity in host cells |
| DK2358882T3 (da) * | 2008-11-18 | 2017-11-06 | Commw Scient Ind Res Org | Enzymer og fremgangsmåder til fremstilling af omega-3 fedtsyrer |
| DK2951309T3 (en) * | 2013-02-01 | 2019-04-23 | Selexis Sa | INCREASED TRANSGEN EXPRESSION AND PROCESSING |
| EP3030656B1 (en) * | 2013-08-06 | 2018-08-22 | Lonza Biologics Plc. | Means and methods for the generation of mammalian producer cells for the production of recombinant proteins |
| WO2017191165A1 (en) | 2016-05-03 | 2017-11-09 | Lonza Ltd | Modulation of lipid metabolism for protein production |
-
2017
- 2017-05-03 WO PCT/EP2017/060484 patent/WO2017191165A1/en not_active Ceased
- 2017-05-03 CA CA3023038A patent/CA3023038C/en active Active
- 2017-05-03 EP EP17724770.7A patent/EP3452608B1/en active Active
- 2017-05-03 CN CN201780039286.6A patent/CN109415748A/zh active Pending
- 2017-05-03 AU AU2017259010A patent/AU2017259010B2/en active Active
- 2017-05-03 SG SG11201809626PA patent/SG11201809626PA/en unknown
- 2017-05-03 ES ES17724770T patent/ES2938626T3/es active Active
- 2017-05-03 EA EA201892503A patent/EA201892503A1/ru unknown
- 2017-05-03 IL IL262646A patent/IL262646B/en unknown
- 2017-05-03 MX MX2018013499A patent/MX2018013499A/es unknown
- 2017-05-03 IL IL294241A patent/IL294241B2/en unknown
- 2017-05-03 US US15/585,570 patent/US10655111B2/en active Active
- 2017-05-03 JP JP2018557851A patent/JP6959942B2/ja active Active
- 2017-05-03 KR KR1020187034453A patent/KR102536221B1/ko active Active
-
2020
- 2020-03-13 US US16/818,162 patent/US11434474B2/en active Active
-
2022
- 2022-07-27 US US17/815,465 patent/US20230047838A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| MX2018013499A (es) | 2019-09-11 |
| US20230047838A1 (en) | 2023-02-16 |
| KR20190003651A (ko) | 2019-01-09 |
| CA3023038A1 (en) | 2017-11-09 |
| AU2017259010B2 (en) | 2021-04-22 |
| IL262646B (en) | 2022-08-01 |
| IL294241B2 (en) | 2023-10-01 |
| KR102536221B1 (ko) | 2023-05-23 |
| US20170321194A1 (en) | 2017-11-09 |
| US10655111B2 (en) | 2020-05-19 |
| IL294241B1 (en) | 2023-06-01 |
| WO2017191165A1 (en) | 2017-11-09 |
| EA201892503A1 (ru) | 2019-09-30 |
| IL294241A (en) | 2022-08-01 |
| AU2017259010A1 (en) | 2018-11-22 |
| US11434474B2 (en) | 2022-09-06 |
| IL262646A (en) | 2018-12-31 |
| EP3452608A1 (en) | 2019-03-13 |
| JP6959942B2 (ja) | 2021-11-05 |
| BR112018072434A2 (pt) | 2019-02-19 |
| CN109415748A (zh) | 2019-03-01 |
| EP3452608B1 (en) | 2022-11-30 |
| SG11201809626PA (en) | 2018-11-29 |
| ES2938626T3 (es) | 2023-04-13 |
| US20200231944A1 (en) | 2020-07-23 |
| JP2019514399A (ja) | 2019-06-06 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20200707 |
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| MPN | Maintenance fee for patent paid |
Free format text: FEE DESCRIPTION TEXT: MF (PATENT, 8TH ANNIV.) - STANDARD Year of fee payment: 8 |
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| U00 | Fee paid |
Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED Effective date: 20250422 |
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| U11 | Full renewal or maintenance fee paid |
Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL Effective date: 20250422 |
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| MPN | Maintenance fee for patent paid |
Free format text: FEE DESCRIPTION TEXT: MF (PATENT, 9TH ANNIV.) - STANDARD Year of fee payment: 9 |
|
| U00 | Fee paid |
Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED Effective date: 20260421 |
|
| U11 | Full renewal or maintenance fee paid |
Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL Effective date: 20260421 |