KR102527041B1 - Cosmetic composition for promoting lipid and collagen biosynthesis containing extracts of arctium lappa seed and lupine peptide - Google Patents

Abstract

The present invention is a cosmetic composition containing a lupine peptide extract and a raspberry extract to improve skin elasticity. More specifically, the lupine peptide extract promotes the synthesis of collagen and the extract promotes the synthesis of ceramide, cholesterol, and triglyceride By doing so, biosynthesis of proteins and lipids is performed simultaneously to provide a cosmetic composition that excellently improves the skin elasticity effect and prevents skin aging by suppressing the occurrence of wrinkles.

Description

COSMETIC COMPOSITION FOR PROMOTING LIPID AND COLLAGEN BIOSYNTHESIS CONTAINING EXTRACTS OF ARCTIUM LAPPA SEED AND LUPINE PEPTIDE}

The present invention relates to a cosmetic composition containing a lupine peptide extract and an extract of Raisinus japonica as active ingredients to impart elasticity to the skin. More specifically, the lupine peptide extract promotes the biosynthesis of collagen, and the extract of Astragalus chinensis promotes the biosynthesis of cholesterol and triglyceride, so that proteins and lipids are biosynthesized at the same time, and the effect of improving skin elasticity is more effective than using each extract alone. It relates to enhanced cosmetic compositions.

The skin is a body organ that plays an important role in maintaining homeostasis, such as regulating body temperature, while being a primary barrier that protects the skin from the external environment. Such skin is damaged by various internal and external stimuli such as aging, ultraviolet rays, and stress. As a result, skin elasticity is lost, skin atrophy, and skin regeneration ability is lowered.

Components that directly affect skin elasticity are protein components such as collagen and elastin, and lipid components such as cholesterol and ceramide. If these ingredients are sufficiently synthesized and function properly, the skin can also be maintained as elastic and healthy skin. This has a direct effect on the occurrence of wrinkles.

For this reason, a cosmetic composition that promotes collagen synthesis (Korean Patent Publication No. 10-2010-0061089, Korean Patent Publication No. 2000-0018467) or a cosmetic composition that promotes lipid biosynthesis (Korean Patent Publication No. 10-2014-0076758 Ho), efforts to improve skin elasticity and prevent skin aging have been steadily made.

The present inventors have completed the present invention by confirming a cosmetic composition that promotes collagen and lipid biosynthesis and has excellent skin elasticity improvement effect by containing a lupine peptide extract and a raspberry extract.

Therefore, the present invention prevents skin aging such as wrinkles by promoting the biosynthesis of protein components and lipid components constituting the skin at the same time using a lupine peptide extract and an extract of Raisinus japonica to greatly enhance the effect of improving skin elasticity, thereby preventing skin aging such as wrinkles and the symptoms It is an object to provide a cosmetic composition that further lasts.

The above object is achieved by a cosmetic composition for improving skin elasticity containing a lupine peptide extract and a raspberry extract as active ingredients.

Preferably, the cosmetic composition has an excellent skin elasticity improvement effect and anti-aging effect by increasing collagen and lipid biosynthesis.

Preferably, the cosmetic composition is characterized by increasing the expression level of collagen, cholesterol, or triglyceride.

In addition, preferably, each of the lupine peptide extract and the coriander extract is characterized in that they are contained in an amount of 0.00001 to 30.0% by weight based on the total weight of the cosmetic composition.

The formulation of the composition according to the present invention is not particularly limited, and may be in a form suitable for use. For example, it can be prepared in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, cleansing, oils, powder foundations, pack massage creams and sprays.

The cosmetic composition according to the present invention has excellent skin safety and has no side effects on the skin, and is excellent in preventing skin aging such as skin elasticity improvement effect and wrinkle improvement effect.

1 is a graph confirming lipid biosynthesis by a lupine peptide extract and a raspberry extract.
Figure 2 is a graph confirming the change in skin elasticity when using a cosmetic composition containing a lupine peptide extract and a raspberry extract.

All technical terms used in the present invention, unless otherwise defined, have the following definitions and correspond to the meanings commonly understood by those of ordinary skill in the art related to the present invention. In addition, although preferred methods or samples are described in this specification, those similar or equivalent thereto are also included in the scope of the present invention.

The term “about” means a reference amount, level, value, number, frequency, percentage, dimension, size, amount, weight or length of 30, 25, 20, 25, 10, 9, 8, 7, 6, 5, means an amount, level, value, number, frequency, percentage, dimension, size, quantity, weight or length that varies by 4, 3, 2 or 1%.

Throughout this specification, unless the context requires otherwise, the terms "comprise" and "comprising" include a given step or element, or group of steps or elements, but any other step or element, or It is to be understood that steps or groups of components are not excluded.

The present invention relates to a cosmetic composition for improving skin elasticity containing a lupine peptide extract and an extract of Raisinus japonica as active ingredients.

The cosmetic composition promotes the synthesis of collagen, which is a protein component, by the lupine peptide extract, and increases the biosynthesis of cholesterol, triglyceride, or ceramide, which are lipid components, by the extract of Azalea extract, thereby enhancing skin elasticity and suppressing the occurrence of wrinkles, thereby reducing skin aging prevent

Lupin is a leguminous plant that produces seeds (grains), and the cultivation history of lupin is a crop of more than 2,000 years. Because lupine grows well in barren or acidic soil, it is also cultivated for greening or silage, but most lupine is toxic or has a high alkaloid content that reduces palatability. Because these alkaloids taste bitter, they function to protect themselves from pests or livestock grazing. In the past, alkaloids were removed by soaking the seeds, but in the 1920s, a German breeder developed low alkaloid or sweet lupine varieties that can be eaten immediately by humans or livestock, and are still in development. Lupine seeds (grains) are rich in protein with high nutritional value, but relatively low in fat. Similar to those derived from soybeans (hereafter referred to as "soybean oil" and "soybean milk products"), there have been previous attempts to process lupine grains to produce livestock feed or dairy substitute products such as milk. In addition, it can replace many parts of human food, and it is used to increase the nutritional value of the product due to its high protein and carotene. The skin and powder of lupine are used as ingredients in pasta and bread, and are also used in crunches, snacks, baby formula, porridge and salads.

The lupine peptide extract used in the present invention used a hydrolyzed lupine peptide extract with the raw material name "ACTIMP® POWDER BIO" from EXPANSCIENCE, France. Preferably, the lupine peptide extract can be prepared by the method described in Korean Patent Publication No. 2017-0026401.

The content of the lupine peptide extract is contained in an amount of 0.00001 to 30.0% by weight based on the total weight of the composition, more preferably 0.001 to 1.0% by weight based on the total weight. If the content of the extract is less than 0.00001% by weight, no effective effect appears, and if it exceeds 30.0% by weight, the degree of improvement in the increase in the content is insignificant, and there are problems with safety and stability of the formulation, and it is not economical. .

Burdock is 50~150cm high, straight roots grow up to 30~60cm, and a stem comes out from the end. The outer surface is dark green, but white downy hairs grow densely on the back side, and there are tooth-shaped sawtooth edges. Flowers bloom in July-August, and are black purplish, and douhwa hangs in corymbose at the end of branches. The guns are round, the guns are needle-shaped, and the ends are like hooks. Flowers are tubular, seeds are black, and hairs are brown. The fruit is an achene and ripens in September. Burdock root is known to contain inulin and some palmitic acid. In Europe, it is used as a diuretic and diaphoretic, and the seeds are used as a diuretic when there is swelling, and as an antidote for sore throat and poisonous insects. The seeds of burdock are also called Woobangja, and are also used as medicinal materials that have the effect of dispersing wind heat, dispersing waste, causing headaches to sprout, relieving swelling, and detoxifying.

The extract used in the present invention was prepared with reference to the following specific preparation examples, and the manufacturing method is described as follows: After washing with purified water, and then drying and crushing into small pieces, 1 of its dry weight -Add 10 times lower alcohol. After extracting at a certain temperature for a certain period of time and filtering, it is dried with a rotary vacuum evaporator.

The coriander extract is characterized in that it contains 0.00001 to 30.0% by weight, respectively, more preferably, 0.001 to 10% by weight based on the total weight of the composition. If the content of the extract is less than 0.00001% by weight, no effective effect appears, and if it exceeds 30.0% by weight, the degree of improvement in the increase in the content is insignificant, and there are problems with safety and stability of the formulation, and it is not economical. .

According to one embodiment of the present invention, the cosmetic composition preferably comprises a lupine peptide extract and a bellflower extract in a weight ratio of 1:3 to 3:1. More preferably, it may include the lupine peptide extract and the chinensis extract in a weight ratio of 1:1. The cosmetic composition of the present invention has more excellent collagen biosynthesis and lipid biosynthesis effects than when the lupine peptide extract and the chinensis extract are mixed and included in the weight ratio above.

The cosmetic composition containing the lupine peptide extract and the Astragalus extract may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, It can be made into a formulation of soap, cleansing, oil, powder foundation, pack massage cream and spray.

Preparation Example 1 : Preparation of Woobangja Extract

The method for preparing the extract of Raisinus japonica used in the present invention is as follows. After washing 100 g of Brussels sprouts with purified water, drying and crushing into small pieces, 70% lower alcohol in an amount of 1 to 10 times its dry weight is added thereto. After extracting at 15-35° C. for 5 days, the mixture was filtered with a 300 mesh filter cloth, again filtered with a Whatman No. 5 filter paper, and then dried with a rotary vacuum evaporator to obtain an extract having a dry weight of 28.7 g.

Experimental Example 1 : Measurement of collagen biosynthesis promoting effect

In order to examine the effect of promoting collagen biosynthesis, human normal fibroblasts were inoculated into each well of a 96-well microplate to become 1X10 4 cells, and DMEM: dulbecco,s Modified Eagle's Medium) culture medium for 24 hours. After culturing, the extract and lupine peptide extract, or the extract obtained by mixing the extract and lupine peptide extract at a ratio of 1:1 (weight ratio) were replaced with serum-free DMEM medium adjusted to a final concentration of 100 μg/ml. 48 Incubate more time. 50 μg/ml of ascorbic acid was added 24 hours prior to the end of culture to promote collagen synthesis. After culturing, each well was washed, replaced with serum-free DMM medium, and cultured for another 24 hours. After culturing, the supernatant of each well was collected and the amount of newly synthesized collagen was measured as the amount of PICP using a kit (Kit, Takara, Kyoto, Japan) for the amount of procollagen type I C-peptide (PICP). The amount of PICP was converted into ng/2X10 4 cells, and the synthesis effect of collagen was shown in Table 1 below. As confirmed in Table 1 below, compared to the control group, the amount of collagen synthesis in the extract of the coriander extract did not significantly increase, but the amount of collagen synthesis in the lupine peptide extract increased significantly compared to the control group. ), it can be seen that the amount of collagen synthesis is increased compared to the case of treating the lupine peptide extract alone.

division Amount of collagen synthesis (ng/2X10 ⁴ ) control group 135 Woobangja Extract 140 Lupine Peptide Extract 184 Woobangja extract + lupine peptide extract (1:1 weight ratio) 215

Experimental Example 2 : Cholesterol Measurement

Human sebaceous cell line SZ95 cells were prepared by Dr. Benjamin Franklin, The Free University of Berlin University Medical center, Germany. Human immortalized sebocyte (SZ95) cells received from Zouboulis were cultured in a CO2 incubator (37°C, 5% CO2) using Dulbecco's modified eagle medium (DMEM)/F12 (1:1) supplemented with 10% FBS. And the culture medium was replaced every 48 hours.

SZ95 cells cultured in this way were dispensed into a 10 cm culture container, and samples separately treated with lupine peptide extract (30ug/ml) and chamomile extract (30ug/ml) or lupine peptide extract (15ug/ml) and chamomile extract (15ug/ml) ) were prepared and then cultured for 5 days and cholesterol was quantified. In the method for quantifying cholesterol, cells cultured for 5 days were treated with a lysis buffer (EDTA 10 μL, 100 mM SPB solution, PMSF 1 μL, Triton X-100 10 μL) and disaggregated by ultrasonic waves. A chloroform:methanol (2:1) solution was added to the cell lysate, mixed, centrifuged (2000 rpm, 15 minutes), and the lower layer was collected and concentrated with nitrogen gas (N2). After dissolving 300 μL of ethanol in the concentrated sample, total cholesterol kit (AM 202-K) was used to measure the total cholesterol. 100 μL of sample was added to 1 mL of enzyme solution, mixed, and incubated at 37° C. for 5 minutes, and then absorbance was measured with an ELISA reader at a wavelength of 500 nm. Cholesterol in the standard solution was set to 3 mg/mL by adding 20 μL of the standard solution to 3 mL of the enzyme solution. The amount of cholesterol was calculated by Equation 1 below.

The amount of cholesterol measured is shown in Table 2. Looking at Table 2, when the control group was 0.81mg / ml and treated with the extract of the raspberry extract alone (0.88 mg / ml), or when the lupine peptide extract was treated alone (0.83 mg / ml), the extract and the peptide extract of the lupine were more It can be seen that cholesterol synthesis increases when treated together (1.23 mg/ml).

division Total cholesterol (mg/ml protein) control group 0.81 Woobangja Extract 0.88 Lupine Peptide Extract 0.83 Woobangja extract + lupine peptide extract (1:1 weight ratio) 1.23

Experimental Example 3 : Triglyceride measurement

SZ95 cells cultured in Experimental Example 2 were dispensed into a 10 cm culture container, and samples separately treated with lupine peptide extract (30ug/ml) and chamomile extract (30ug/ml), lupine peptide extract (15ug/ml) and chamomile extract ( 15ug/ml) were prepared, and then cultured for 5 days and triglyceride was quantified. In the triglyceride quantification method, cells cultured for 5 days were treated with lysis buffer (EDTA 10uL, 100mM SPB solution, PMSF 1uL, Triton X-100 10uL) and disaggregated by ultrasonic waves. A chloroform:methanol (2:1) solution was added to the cell lysate, mixed, centrifuged (2000 rpm, 15 minutes), and the lower layer was collected and concentrated with nitrogen gas (N2). After dissolving the concentrated sample in 300 μl of ethanol, triglycerides were measured using a Triglycerides kit (GPO-PAP). 100 μL of the sample was added to 1 mL of the enzyme solution, mixed, and incubated at 37° C. for 5 minutes, and the absorbance was measured with an ElISA Reader at a wavelength of 546 nm. The triglyceride of the standard solution was set to 3 mg/ml by adding 20 μl of the standard solution to 3 mL of the enzyme solution. The amount of triglyceride was calculated by Equation 2 below.

The amount of triglyceride measured is shown in Table 3. Looking at Table 3, when the control group was 1.97 mg / ml and treated with woobangja alone (2.33 mg / ml), or when treated with lupine peptide alone (2.11 mg / ml), when treated with woobangja and lupine peptide together (2.98 mg/ml) it can be seen that triglyceride synthesis is increased.

division Total triglycerides (mg/ml protein) control group 1.97 Woobangja Extract 2.33 Lupine Peptide Extract 2.11 Woobangja extract + lupine peptide extract (1:1 weight ratio) 2.98

Experimental Example 4 : Measurement of lipid biosynthesis

Human normal skin cells, keratinocytes (Korea Cell Line Bank, Korea) were inoculated into 48-well microplates (Nunc, Denmark) to be 1X10 ⁶ cells per well, and cultured in DMEM medium (Sigma, United States of America) and at 37°C for 24 After culturing for a period of time, lipid production was measured by Nile red staning & flow cytometry.

The cultured keratinocytes were treated with 12.5 μg/ml of a 1:1 (weight ratio) mixture of the chinensis extract, the lupine peptide extract, and the two extracts and 200 μg/ml of linolenic acid as a positive control, respectively, and cultured for 48 hours. After collecting keratinocytes from each month, they were stained with 1 μM Nile red solution for 10 minutes, and fluorescence intensity was measured at 552/636 (Ex/Em) nm. The measurement results are shown in FIG. 1 . Referring to Figure 1, the lipid production (99.12) of Woobangja was superior to that of the positive control, linolenic acid 200 μg / ml (93.54), but the lupine peptide extract (40.12) had less lipid production than linolenic acid. In addition, it was confirmed that lipid production was more increased in case of mixing the coriander extract and the lupine peptide extract (109.33) than in the case of using the coriander extract alone.

Formulation Example 1 : Preparation of a Composition Containing Lupine Peptides and Corianja Extract

Formulation Example 1 containing a lupine peptide extract and a raspberry extract was prepared, and for comparison of efficacy, a composition containing the lupine peptide extract alone (Comparative Formulation Example 1) and a composition containing the Astragalus raspberry extract alone (Comparative Formulation Example 2 ), and a composition (Comparative Formulation Example 3) not including all of the extracts are shown in Table 4 below.

division Raw material (% by weight) Formulation Example 1 Comparative Formulation Example 1 Comparative Formulation Example 2 Comparative Formulation Example 3 One Lupine Peptide Extract 0.1 0.2 - - 2 Woobangja Extract 0.1 - 0.2 - 3 Purified water To 100 To 100 To 100 To 100 4 glycerin 2 2 2 2 5 1,3-butylene glycol 3 3 3 3 6 xanthan gum 0.02 0.02 0.02 0.02 7 antiseptic Appropriate amount Appropriate amount Appropriate amount Appropriate amount 8 Spices Appropriate amount Appropriate amount Appropriate amount Appropriate amount 9 dicaprylyl carbonate 2 2 2 2 10 Polyacrylate Crosspolymer-6 0.3 0.3 0.3 0.3 11 Polyglyceryl-3methylglucose distearate 2 2 2 2 12 Caprylic/Capric Triglyceride 3 3 3 3 Sum 100 100 100 100

Experimental Example 5 : Skin elasticity effect of lupine peptide extract and Azalea extract

The creams prepared in Formulation Example 1 and Comparative Formulation Examples 1 to 3 were applied to the skin to measure the skin elasticity effect. Skin elasticity measurement was measured in the same area before and after using the test product using Cutometer MPA580 (Courage+Khazaka electronic GmBH, Germany). R2 (skin re-deformation force) value was used as skin elasticity evaluation data. The R2 value is the overall elasticity, and the closer the value is to 1, the more elastic the skin is.

As shown in Figure 2, the creams prepared in Formulation Example 1 and Comparative Formulation Examples 1 to 3 were applied to the skin, and the skin elasticity was measured before, after 1 week, and after 2 weeks, respectively. Compared to Comparative Formulation Example 3, which did not contain only the lupine peptide extract, Comparative Formulation Example 1 or Comparative Formulation Example 2, which contained only the lupine peptide extract, showed an increase in skin elasticity after 1 week and 2 weeks, respectively. It can be seen that formulation example 1 containing all extracts increases skin elasticity more than comparative formulation examples 1 or 2 containing only each extract.

Claims (5)

A cosmetic composition for improving skin elasticity containing a mixture obtained by mixing a lupine peptide extract and a raspberry extract in a weight ratio of 1:3 to 3:1 as an active ingredient. The cosmetic composition for improving skin elasticity according to claim 1, wherein the cosmetic composition promotes the synthesis of collagen and lipid components. The cosmetic composition for improving skin elasticity according to claim 2, wherein the promotion of lipid component synthesis increases the expression level of cholesterol or triglyceride. The cosmetic composition for improving skin elasticity according to any one of claims 1 to 3, wherein the cosmetic composition comprises 0.00001 to 30.0% by weight of each of the lupine peptide extract and the coriander extract in an amount of 0.00001 to 30.0% by weight based on the total weight of the cosmetic composition . According to any one of claims 1 to 3, the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing, oil, powder foundation, pack massage cream and spray Cosmetic composition for improving skin elasticity, characterized in that the formulation of.

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