KR102504317B1 - 재조합 단백질을 생성시키는 방법 - Google Patents

재조합 단백질을 생성시키는 방법 Download PDF

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KR102504317B1
KR102504317B1 KR1020187034736A KR20187034736A KR102504317B1 KR 102504317 B1 KR102504317 B1 KR 102504317B1 KR 1020187034736 A KR1020187034736 A KR 1020187034736A KR 20187034736 A KR20187034736 A KR 20187034736A KR 102504317 B1 KR102504317 B1 KR 102504317B1
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recombinant
sequence
expression
signal sequence
recombinant protein
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KR20190003694A (ko
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로빈 알렉산드라 엠민스
개리 브라이언 핀카
미셸 호어
앨런 피터 루이스
윌리엄 존 케네스 루이스
아담 다니엘 매키날리
마크 우덴
이오아니스 불가리스
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글락소스미스클라인 인털렉츄얼 프로퍼티 디벨로프먼트 리미티드
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
KR1020187034736A 2016-05-06 2017-05-04 재조합 단백질을 생성시키는 방법 Active KR102504317B1 (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201662332636P 2016-05-06 2016-05-06
US62/332,636 2016-05-06
US201662338045P 2016-05-18 2016-05-18
US62/338,045 2016-05-18
PCT/EP2017/060654 WO2017191255A1 (en) 2016-05-06 2017-05-04 Method of producing a recombinant protein

Publications (2)

Publication Number Publication Date
KR20190003694A KR20190003694A (ko) 2019-01-09
KR102504317B1 true KR102504317B1 (ko) 2023-02-24

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KR1020187034736A Active KR102504317B1 (ko) 2016-05-06 2017-05-04 재조합 단백질을 생성시키는 방법

Country Status (14)

Country Link
US (1) US11098312B2 (https=)
EP (1) EP3452496A1 (https=)
JP (2) JP2019516371A (https=)
KR (1) KR102504317B1 (https=)
CN (1) CN109071611B (https=)
AU (1) AU2017260277A1 (https=)
BR (1) BR112018072043A2 (https=)
CA (1) CA3023025A1 (https=)
IL (1) IL262544A (https=)
MX (1) MX2018013562A (https=)
RU (1) RU2742006C2 (https=)
SG (1) SG11201808976WA (https=)
TW (1) TWI794170B (https=)
WO (1) WO2017191255A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7517707B2 (ja) * 2019-02-15 2024-07-17 オンコシミス バイオテック プライベート リミテッド タンパク質分泌増強のための細菌発現ベクター
KR102094480B1 (ko) 2019-05-27 2020-04-01 (주)엘에스케이화인텍스 재사용 가능한 미세 먼지 항균 마스크
CN110551199B (zh) * 2019-09-26 2022-01-25 杭州纽龙日尚生物制品有限公司 一种提高工程菌分泌表达重组人表皮生长因子的方法
KR20240021234A (ko) * 2021-06-10 2024-02-16 펠리칸 테크놀로지 홀딩스, 인크. 재조합 단백질 발현을 위한 박테리아 숙주

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009021548A1 (en) * 2007-08-10 2009-02-19 Wacker Chemie Ag Expression of full length igg and secretion into the culture medium of prokaryotic cells
US20140244228A1 (en) 2012-09-19 2014-08-28 Agency For Science, Technology And Research Codon optimization of a synthetic gene(s) for protein expression

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005538731A (ja) * 2002-09-20 2005-12-22 シーワイエム１ピー エイ／エス 宿主細胞からの組換えポリペプチドの産生を高める方法
GB0426160D0 (en) * 2004-11-29 2004-12-29 Era Plantech S L Production of proteins
WO2007089093A1 (en) * 2006-01-31 2007-08-09 Republic Of Korea (Republic Of National Fisheries Research And Development Institute) Production of a soluble native form of recombinant protein by the signal sequence and secretional enhancer
GB0602173D0 (en) 2006-02-03 2006-03-15 Avecia Ltd Expression system
RU2488635C1 (ru) 2012-03-22 2013-07-27 Государственное бюджетное образовательное учреждение высшего профессионального образования Первый Московский государственный медицинский университет им. И.М. Сеченова Министерства здравоохранения и социального развития Российской Федерации (ГБОУ ВПО Первый МГМУ им. И.М. Сеченова Минздравсоцразвития Способ получения рекомбинантного антиангиогенного полипептида

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009021548A1 (en) * 2007-08-10 2009-02-19 Wacker Chemie Ag Expression of full length igg and secretion into the culture medium of prokaryotic cells
US20140244228A1 (en) 2012-09-19 2014-08-28 Agency For Science, Technology And Research Codon optimization of a synthetic gene(s) for protein expression

Also Published As

Publication number Publication date
US11098312B2 (en) 2021-08-24
CN109071611A (zh) 2018-12-21
JP2022070902A (ja) 2022-05-13
EP3452496A1 (en) 2019-03-13
BR112018072043A2 (pt) 2019-02-12
RU2018137305A3 (https=) 2020-06-08
RU2018137305A (ru) 2020-06-08
KR20190003694A (ko) 2019-01-09
SG11201808976WA (en) 2018-11-29
WO2017191255A1 (en) 2017-11-09
TW201805298A (zh) 2018-02-16
IL262544A (en) 2018-12-31
RU2742006C2 (ru) 2021-02-01
US20190177734A1 (en) 2019-06-13
JP2019516371A (ja) 2019-06-20
CA3023025A1 (en) 2017-11-09
TWI794170B (zh) 2023-03-01
CN109071611B (zh) 2022-11-01
AU2017260277A1 (en) 2018-11-22
MX2018013562A (es) 2019-03-14

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