KR102499077B1 - Method for producing a wild ginseng cultured root extract and a cosmetic composition for wrinkle improvement using a ginsenoside complex obtained from a wild ginseng cultured root extract - Google Patents

Abstract

The present invention is a peptide complex prepared from a plurality of peptides contained in a microalgae extract and a ginsenoside complex obtained from wild ginseng cultured root extract with increased specific ginsenoside content as main components to exhibit moisturizing, whitening and wrinkle improvement effects It relates to a method for producing cultured wild ginseng cultured root extract and a cosmetic composition for improving wrinkles using a ginsenoside complex obtained from cultured wild ginseng cultured root extract.

Description

Method for producing a wild ginseng cultured root extract and a cosmetic composition for wrinkle improvement using a ginsenoside complex obtained from a wild ginseng cultured root extract}

The present invention is a peptide complex prepared from a plurality of peptides contained in a microalgae extract and a ginsenoside complex obtained from wild ginseng cultured root extract with increased specific ginsenoside content as main components to exhibit moisturizing, whitening and wrinkle improvement effects It relates to a method for producing cultured wild ginseng cultured root extract and a cosmetic composition for improving wrinkles using a ginsenoside complex obtained from cultured wild ginseng cultured root extract.

Human skin is composed of an epidermis layer, a dermis layer, and skin tissue, and the dermis layer below the epidermis is composed of collagen, elastin, and hyaluronic acid. In particular, collagen is a connective tissue composed of amino acids, a type of protein. As human aging progresses, the amount of dense collagen fibers decreases, and as they become loose, elasticity decreases, resulting in sagging skin or deep wrinkles on the face, making the face look rugged and wide. do. Of course, aging is a phenomenon common to all humans over time, but for many humans who value beauty, aging is like a disaster.

Methods for suppressing aging are constantly being studied, and in particular, methods for increasing the collagen are also being studied together. There are also various ingredients that are attracting attention in anti-aging research. In particular, even in the case of peptides, which are substances made by combining two or more amino acids, they are attracting attention in that they can directly supplement collagen in the dermal layer. In the case of peptides, the particle size is smaller than that of protein, and it has the advantage of penetrating deeply into the dermal layer, so a better skin improvement effect can be expected.

For reference, in the case of Korean Patent Laid-open Publication No. 10-2017-0003903, a cosmetic composition with excellent anti-aging or anti-wrinkle effects was provided through complementary actions of vitamins and peptides. However, in the case of niacinamide (vitamin B) used in the above patent, when more than a certain component is included, it may precipitate in the composition and reduce the stability of external preparations, and in the case of ascorbyl glucoside (vitamin C) as a vitamin C derivative There was a problem that the activity was weak.

Korean Patent Registration No. 10-1803283

The present invention is to solve the above problems, a peptide complex prepared by mixing each of the peptides obtained from microalgae, and a ginsenoside complex obtained from wild ginseng cultured root extract with increased specific ginsenoside content as main components The purpose is to provide a cosmetic composition for improving wrinkles using a method for preparing wild ginseng cultured root extract and a ginsenoside complex obtained from wild ginseng cultured root extract, which can exhibit moisturizing, whitening and wrinkle-improving effects.

As a means for solving the above problems, the configuration of the present invention comprises 0.5 to 10.6 parts by weight of the peptide complex, 40 to 90 parts by weight of purified water, 0.1 to 5 parts by weight of a moisturizer, 0.001 to 10 parts by weight of ceramide and 0.01 to 2 parts by weight of a preservative. include

The peptide complex,

A first peptide obtained from a Chlorella vulgaris extract, a second peptide obtained from a Dunaliella salina extract, a third peptide obtained from a Schizochytrium aggregatum extract, Skizokit A fourth peptide obtained from a Schizochytrium limacinum extract, a fifth peptide obtained from a Schizochytrium minutum extract, a sixth peptide obtained from a Crypthecodinium cohnii extract, and Characterized in that the seventh peptide obtained from the Haematococus pluviallis extract was mixed in a weight ratio of 1: 2: 2: 1: 1: 2: 2,

The cosmetic composition further comprises 0.001 to 15 parts by weight of the ginsenoside complex,

The ginsenoside complex,

Ginsenosides Rg1 and Rg2 are obtained from wild ginseng cultured roots, respectively, and the obtained ginsenosides Rg1 and Rg2 are mixed in a weight ratio of 1 to 3: 1 to 3, respectively.

The ginsenoside complex,

Wild ginseng cultured roots are crushed and subjected to primary steaming for 8 hours at a temperature of 30°C and humidity of 40%. The primary steamed cultured wild ginseng cultured roots are placed in a freeze dryer and first dried at -50°C for 10 hours, followed by primary drying. The cultured wild ginseng cultured roots were subjected to secondary steaming for 8 hours at a temperature of 30 ° C and humidity of 40%, and the secondary steamed cultured wild ginseng cultured roots were placed in a freeze dryer and dried a second time at -50 ° C for 10 hours, and then 60 to 120 mesh (mesh) to produce wild ginseng cultured root powder,

An extraction mixture was prepared by adding 1 part by weight of wild ginseng cultured root powder to 100 parts by weight of an aqueous solution of pH 4.8 citric acid, and the extraction mixture was put into a high-pressure reactor for 8 hours, and then the extraction mixture was recovered. From the recovered extraction mixture, ginsenoside Rg1, It is characterized in that each of Rg2 is obtained and mixed.

The present invention is a peptide complex prepared from a plurality of peptides contained in a microalgae extract and a ginsenoside complex obtained from wild ginseng cultured root extract with increased specific ginsenoside content as main components to prevent aging, moisturize, whiten and improve wrinkles effect can be shown.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings in order to explain in detail enough that a person skilled in the art to which this invention pertains can easily practice this invention. Other objects and operational advantages, including the purpose, operation, and effect of this invention, will become clearer from the description of the preferred embodiments.

For reference, the embodiments disclosed herein are only presented by selecting the most preferred embodiments to help those skilled in the art to understand among various possible examples, and the technical spirit of the present invention is not necessarily limited or limited only by the presented embodiments. No, and various changes, additions, and changes including equivalents and substitutes are possible within the scope without departing from the technical spirit of the present invention.

In addition, the terms or expressions of words used in the specification and claims are defined based on the principle that the inventor can appropriately define the concept of terms in order to best explain his or her invention. It should not be construed as limited only to the dictionary meaning, and should be interpreted as meaning and concept corresponding to the technical spirit of the present invention. As an example, singular expressions include plural expressions unless the context clearly dictates otherwise.

A cosmetic composition for whitening and wrinkle improvement comprising a peptide complex obtained from a microalgae extract and a ginsenoside complex obtained from wild ginseng cultured root extract according to the present invention contains 0.5 to 10.6 parts by weight of the peptide complex and 0.001 to 15 parts by weight of the ginsenoside complex. parts by weight, 40 to 90 parts by weight of purified water, 0.1 to 5 parts by weight of a moisturizer, 0.001 to 10 parts by weight of ceramide, and 0.01 to 2 parts by weight of a preservative.

The peptide complex is Chlorella vulgaris extract, Dunaliella salina extract, Schizochytrium aggregatum extract, Schizochytrium limacinum extract, Schizochytrium It is characterized in that each of the peptides obtained from Schizochytrium minutum extract, Crypthecodinium cohnii extract and Haematococus pluviallis extract is a mixed peptide complex.

Schizochytrium aggregatum, Schizochytrium limacinum, and Schizochytrium minutum are Schizochytrium sp., used for DNA production, and Thecodinium cohnii (Crypthecodinium cohnii) is also used for DNA production.

Haematococus pluviallis is a Haematococus sp., and is known to produce astaxanthin, an antioxidant, and is known to be effective in improving blood circulation.

Peptides extracted from these 7 types of microalgae are known to induce activity by acting on nerve cells and induce activation of skin tissue through various signal transduction processes.

Ginsenoside is a type of saponin, a kind of glycoside. there is. Saponin is known as a component that enhances the body's immunity, and ginsenoside, a type of saponin contained in ginseng, red ginseng, wild ginseng, gosam, and black ginseng, is especially famous.

In particular, rare ginsenoside is a word that refers to saponin contained only in ginseng, and is used to distinguish it from saponin of other plants. Rare ginsenosides are smaller in size than general ginsenosides and have structural characteristics that are relatively easy to absorb into the body. In addition, when normal ginsenoside is ingested, a small amount is converted to rare ginsenoside by intestinal microorganisms and absorbed. However, since individual differences are large, taking it in the form of rare ginsenoside is more efficient in terms of absorption rate and bioavailability in the body. there is.

Ginsenosides include ginsenosides Rg1, Rg2, Rg3, Rg4. There are various types such as Rg6, Rb1, Rb2, Rh1, Rh2, Rc, Rd, Re, Ro, etc., and the amount of ginsenoside present depending on the type of plant, cultivation conditions, extraction method, and part of ginseng, red ginseng, and wild ginseng Different types and contents.

Ginsenoside Rg1 is known as a substance that enhances immune function and acts as an anti-fatigue, and Rg2 is known as a substance that inhibits platelet aggregation.

The ginsenoside complex according to the present invention is obtained by obtaining ginsenosides Rg1 and Rg2 from any one of wild ginseng cultured roots, red ginseng, ginseng, black ginseng or high ginseng, which have been subjected to high-pressure enzyme degradation after two times of steaming, respectively, and the obtained ginseng It is characterized in that it is made by mixing senosides Rg1 and Rg2 in a weight ratio of 1 to 3: 1 to 3, respectively.

Hereinafter, methods for preparing the peptide complex and the ginsenoside complex will be described.

(Preparation of peptide complex)

7 species of microalgae Chlorella vulgaris, Dunaliella salina, Schizochytrium aggregatum, Schizochytrium limacinum, Schizochytrium minantum ( Schizochytrium minutum), Crypthecodinium cohnii, and Haematococus pluviallis were subcultured in flasks for 10-30 days by preparing culture conditions and media suitable for each growth temperature and pH. did

In the culture incubator, a light source was installed to facilitate photosynthesis to create a culture environment, and flask culture was performed using a carbon dioxide incubator to maintain the concentration of carbon dioxide in the culture medium at an appropriate level.

Thereafter, the cultured microalgae are centrifuged at 5,000 to 10,000 rpm for 20 to 30 minutes to first remove moisture, and then freeze-dry the microalgae from which moisture has been removed to some extent through a centrifugal separator to secondarily remove residual moisture. did

Methanol, ethanol, isopropanol, butanol, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloromethane, N,N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butylene glycol, propylene glycol, or a mixture of these organic solvents are used, and the active ingredients of herbal medicines can be extracted at room temperature or heated under conditions that are not destroyed or minimized. Depending on the organic solvent to be extracted, the degree of extraction and loss of the active ingredient of the drug may differ, so select and use an appropriate organic solvent. In addition, it can be obtained through various known extraction methods not specifically mentioned herein, such as physical methods, hot water extraction, ethanol extraction, and the like.

In order to extract the peptide absorbed in the organic solvent, it was put into a separator having a filter membrane suitable for the size of the peptide to obtain only the peptide, and a peptide complex was prepared by mixing 7 peptides extracted from each microalgae extract.

Specifically, the peptide complex according to the present invention is a first peptide obtained from Chlorella vulgaris extract, a second peptide obtained from Dunaliella salina extract, Schizochytrium aggregatum ) The third peptide obtained from the extract, the fourth peptide obtained from the Schizochytrium limacinum extract, the fifth peptide obtained from the Schizochytrium minutum extract, Cryptecodinium cony ( The sixth peptide obtained from Crypthecodinium cohnii) extract and the seventh peptide obtained from Haematococus pluviallis extract were added to the stirrer at a weight ratio of 1: 2: 2: 1: 1: 2: 2, respectively. After adding, it can be obtained by stirring at 500 to 1000 rpm for 5 hours to 8 hours.

(Preparation of ginsenoside complex)

First, 5 kg of wild ginseng cultured roots were crushed and subjected to primary steaming for 8 hours at a temperature of 30 ° C and a humidity of 40%. Next, the firstly steamed wild ginseng cultured roots were put in a freeze dryer and first dried at -50 ° C for 10 hours. Subsequently, the firstly dried wild ginseng cultured root was subjected to a second steaming treatment for 8 hours at a temperature of 30 ° C and a humidity of 40%. Next, the wild ginseng cultured roots subjected to secondary steaming were placed in a freeze dryer and secondary drying was performed at -50 ° C for 10 hours.

A natural product powder was prepared by pulverizing each sample dried by a lyophilizer into a size of 60 to 120 mesh. In addition, an aqueous solution of citric acid for use as an extraction solvent was prepared by diluting with distilled water until the pH of a 0.2M citric acid solution reached 4.8. Then, 1 part by weight of natural product powder (cultured wild ginseng cultured root powder) was added to 100 parts by weight of the pH 4.8 citric acid aqueous solution prepared above to prepare an extraction mixture (sample 30mg/citric acid solution 30mL). Based on 30 mL of the above extraction mixture, 50 to 100 μL of Viscozyme (KTN02214) or Pectinex Ultra SP-L (KRN05658) from Novozymes (Bagsvaerd, Denmark), or a mixture of the two enzymes (Viscozyme + Pectinex) is added to the extraction mixture.

The prepared mixture for extraction was administered to a polypropylene bag (Nasco, WHIRL-PAK-4oz.), which is a plastic container for ultra-high pressure reactors, and sealed so that there were no air bubbles to prepare samples for high-pressure enzyme reaction treatment. The high-pressure reactor-only vessel containing the extraction mixture containing the enzyme was placed in a high-pressure reactor (Toyo Koatsu, TFS-2L), and the treatment conditions of the high-pressure reactor were set to a pressure of 100 MPa, a temperature of 50 ° C, and a high-pressure treatment time of 8 hours. and started operating. After 8 hours, the extraction mixture was recovered, and a sample was prepared by adding 45 mL of ethanol based on 30 mL of the extraction mixture. The comparative group was an extract solution of cultured wild ginseng cultured roots extracted by immersion in a water-bath at 45 ° C under atmospheric pressure (0.1 MPa) for 4 hours.

In order to detect the type and content of ginsenoside included in the experimental group, which is the sample prepared by the above method and ultra-high pressure treated sample, and the comparative sample, which is sample treated at atmospheric pressure, the index component of each sample was analyzed according to the method below. .

Analytical procedure

1. Preparation of test solution

1) Precisely weigh about 1 g of the sample, put it in a 250 mL reflux flask, add 50 mL of 50% methanol solution, extract under reflux for 1 hour in a 70-80oC water bath, cool, and centrifuge to take the supernatant (supernatant liquid A) .

2) Add 50 mL of 50% methanol solution to the residue, extract under reflux for 1 hour in a 70-80oC water bath, cool, and centrifuge to obtain supernatant (supernatant B).

3) Combine supernatant A and supernatant B, put them in a 250 mL concentrating flask, and concentrate under reduced pressure at 60℃ or lower.

4) After dissolving the concentrate in 2 mL of a water-acetonitrile (80:20 V/V) mixed solvent, filter it through a 0.45 μm membrane filter and use it as a test solution for HPLC analysis.

2. HPLC analysis

20 μL of the test solution and 20 μL of the standard solution were injected into HPLC and analyzed according to the following conditions to obtain the peak area of each ginsenoside.

1) Column: ODS (4.6 mm × 250 mm) or equivalent or higher

2) Detector: UV (measurement wavelength: 203 nm)

3) Mobile phase: water-acetonitrile mixed solvent (flow rate: 1.6 mL/min)

3) Calculation of ginsenoside content

The amount of each ginsenoside contained in the sample is calculated according to the following formula.

[Equation 1]

Y=S(a*b) / A*0.001

Here, Y is the ginsenoside content, S is the concentration of individual ginsenosides in the test solution (μg/mL), a is the total amount of the test solution (mL), b is the dilution factor, and A is the sample amount (g) do.

The total content of ginsenosides in the sample treated with enzyme treatment and ultra-high pressure, calculated in this way, was 28.34mg/g, which was 22.4% higher than the content of ginsenoside (23.15mg/g) in the sample treated with atmospheric pressure (comparison group). did

In the same way as above, after obtaining ginsenosides Rg1 and Rg2 from wild ginseng cultured roots, respectively, ginsenosides Rg1 and Rg2 were added to a stirrer at a weight ratio of 1-3: 1-3, respectively, and stirred at 500 to 1000 rpm for 5 hours. A ginsenoside complex was prepared by stirring for 8 to 8 hours.

(Preparation of cosmetic composition containing peptide complex and ginsenoside complex)

0.5 to 10.6 parts by weight of the peptide complex, 0.001 to 15 parts by weight of the ginsenoside complex, 40 to 90 parts by weight of purified water, 0.1 to 5 parts by weight of a moisturizer, 0.001 to 10 parts by weight of ceramide and 0.01 to 2 parts by weight of a preservative. manufactured.

The composition of the present invention preferably contains the peptide complex in an amount of 0.5 to 10.6 parts by weight based on the total weight. If the content of the peptide complex is less than 0.5 parts by weight, there is a problem that the melanin production inhibitory effect is lowered, and if it exceeds 10.6 parts by weight, problems in skin safety or formulation may occur.

In this invention, it is preferable to contain the ginsenoside complex in an amount of 0.001 to 15 parts by weight based on the total weight. If the content of the ginsenoside complex is less than 0.001 parts by weight, the efficacy and effect of the above components are insignificant, and if it exceeds 15 parts by weight, problems in skin safety or formulation may occur.

The ceramide is a substance belonging to lipids, and is the most important component among oils and fats contained in skin keratin, and is said to play a large role in forming a skin protective film that acts against the external environment, and also has a function of strengthening the skin lipid barrier. It is known that there is

On the other hand, the cosmetic composition containing the peptide complex derived from the microalgae extract of the present invention is not particularly limited in its formulation, for example, toner, lotion, lotion, gel, water-soluble liquid, cream, essence, mist, water It may have formulations such as heavy oil (O/W) type and water-in-oil (W/O) type. And, in the cosmetic composition of each dosage form, other components other than the peptide complex derived from the microalgae extract can be selected and blended without difficulty by those skilled in the art according to the formulation or purpose of use of other cosmetics.

A cosmetic composition was prepared in the composition ratio shown in Table 1 below using the peptide complex, ginsenoside complex and other auxiliary ingredients according to the present invention.

(Experimental Example 1) Skin improvement effect

In order to confirm the skin improvement effect of the cosmetic composition according to the present invention, a cosmetic composition (Examples 1 to 4, Comparative Examples 1 to 4) was prepared, and a skin improvement evaluation experiment was conducted on a total of 22 subjects in their 20s to 50s. did The subjects were divided into 8 groups (Examples 1 to 4, Comparative Examples 1 to 4), and used once a day for 8 weeks.

First, the moisturizing effect was calculated according to Adolf Fick's Law by calculating the amount of moisture emitted from the skin using a Tewameter. The amount of change before and after using the cosmetic composition was quantified.

Next, the whitening and anti-wrinkle effect was evaluated by having the subject describe the degree of improvement as a score according to the opinion of the subject.

Subsequently, the melanin production inhibitory effect was confirmed through an experiment measuring melanin biosynthesis of the zalatin peptide compound in skin melanocytes.

The cut skin tissue was washed with phosphate buffered saline containing antibiotics (50mg/ml gentamicin, 50mg/ml amphotericin, 50U/ml penicillin, 50μg/streptomycin) and treated with 0.25% Trypsin/EDTA for 5 minutes. After culturing for 15 days in M2 medium (Promocell), a melanocyte selective medium, in an incubator under 5% CO ² and 37 ° C conditions, melanocyte cells were detached with 0.25% Trypsin/EDTA and counted using a hemocytometer to obtain M2 After inoculating 1×10 ⁴ per well in 24-well containing medium (Promocell), incubate at 37℃ until more than 80%d of cells are attached to the well dock. After culturing for 1 day, dopa, a precursor of melanin pigment, was added to 0.08 mM, and then gelatin peptide compounds were added at a test concentration of 0.5% by weight, respectively, and cultured for 3 days, and saline was added to the control group. The cells from which the medium was removed were washed with phosphate buffered saline (PBS), and treated with trypsin to recover the cells. The recovered cells are counted using a hematocytometer, centrifuged at 5,000 to 10,000 for 10 minutes, and then the supernatant is removed to obtain a precipitate. This cell precipitate is dried at 60°C and 10% Intracellular melanin was obtained by adding 100 μl of 1M sodium hydroxide solution containing the mixture in a thermostat at 60°C. The absorbance was measured at 490 using a microplate with this solution, and the melanin content per cell was compared between the control group and the experimental group not treated with the gelatin peptide compound and expressed as %. The results are shown in Table 2 below.

+++: very good improvement effect

++: significant improvement

+: slight improvement

±: no improvement, but no deterioration

-: No improvement effect, rather worsened

As the above results, in the case of the subjects using Examples 1 to 4, an excellent moisturizing effect was exhibited through a high water content, and the degree of skin improvement was evaluated as excellent. Subjects who used the cosmetic compositions of Comparative Examples 1 to 3 showed an evaluation that they did not clearly feel the degree of skin improvement, and in the case of Comparative Example 4, the degree of skin improvement was similar to that of Example.

In addition, in the melanin inhibitory effect test, the melanin content was found to be significantly high only in Comparative Example 1, which did not contain the peptide complex. In particular, in the case of Example 4 containing the peptide complex and the ginsenoside complex, respectively, it was found to be significantly reduced, which increases the skin absorption rate when the contents of the peptide complex and the ginsenoside complex are mixed in a preferred ratio according to the present invention it is judged to have been Therefore, the cosmetic composition of the present invention exhibits a skin improving effect.

(Comparison of efficacy according to mixing ratio of microalgae-derived peptide complex)

In order to more in-depth compare the skin improvement effect of the peptide complex according to the present invention, the efficacy according to the mixing ratio of the microalgae-derived peptide complex was compared. Specifically, the peptide complex according to the present invention is a first peptide obtained from Chlorella vulgaris extract, a second peptide obtained from Dunaliella salina extract, Schizochytrium aggregatum ) The third peptide obtained from the extract, the fourth peptide obtained from the Schizochytrium limacinum extract, the fifth peptide obtained from the Schizochytrium minutum extract, Cryptecodinium cony ( The sixth peptide obtained from Crypthecodinium cohnii) extract and the seventh peptide obtained from Haematococus pluviallis extract were mixed in a weight ratio of 1: 2: 2: 1: 1: 2: 2, respectively. Cosmetic compositions were prepared in Examples, and cosmetic compositions containing a peptide complex mixed in a ratio different from the above ratio were prepared in Comparative Examples (see Table 3).

Thereafter, a total of 50 adult male and female subjects aged 20 to 50 were divided into 5 groups of 10 subjects, and each cosmetic composition was used on the designated test site for 2 weeks to confirm the degree of skin improvement. The results are shown in Table 4 below.

+++: very good improvement effect

++: significant improvement

+: slight improvement

±: no improvement, but no deterioration

-: No improvement effect, rather worsened

As the above results, the subjects using Comparative Examples 1 to 4 showed evaluation that they did not clearly feel the skin improvement effect, and the subjects using Examples generally evaluated the skin improvement effect as excellent. Therefore, it can be seen that the cosmetic composition according to the present invention has an excellent skin improvement effect when each peptide is mixed in the ratio according to the embodiment.

(Comparison of melanin production inhibitory effect according to the components of the ginsenoside complex)

On the other hand, in order to compare the skin improvement effect improvement performance of the ginsenoside complex according to the present invention more in-depth, according to the present invention, ginsenosides Rg1 and Rg2 were mixed in a weight ratio of 1: 2, respectively. A cosmetic composition was prepared as an experimental example, and a cosmetic composition containing a composite in which ginsenosides Rg1 and Rg2 were mixed in a weight ratio of 1: 1 was prepared as Comparative Example 1, and ginsenosides Rg1 and Rg2 were mixed in a weight ratio of 1: 3, respectively. In Comparative Example 2, a cosmetic composition containing a composite obtained by mixing in a weight ratio, and in Comparative Example 3, a cosmetic composition containing a composite obtained by mixing ginsenosides Rg1 and Rg2 in a weight ratio of 2: 1, A cosmetic composition containing a compound in which sides Rg1 and Rg2 were mixed at a weight ratio of 3:1 was prepared in Comparative Example 4, and a cosmetic composition containing a common red ginseng extract was prepared in Comparative Example 5.

Thereafter, the melanin production inhibitory function was assayed at the cellular level by culturing B16 melanoma cells. B16 melanoma cells were cultured in Dulbecco's modified Eagle's Medium (DMEM) containing 10% bovine serum under 5% CO ₂ and 37 °C for 2 days until the number of cells reached 1.0 X 10 ⁸ cells/T25 flask. Then, samples according to Examples and Comparative Examples 1-5 were administered to the cells and cultured for 2 days at 5% CO ₂ and 37 °C.

Thereafter, trypsin was added to collect the cells, and the degree of darkening of the cells was observed. The supernatant of the T25 flask from which melanin was produced and discharged was taken and the absorbance was measured at 475 nm to quantify melanin. It counts and quantifies the amount of melanin produced per unit cell to show the inhibitory effect.

As described above, it can be seen that Examples and Comparative Examples 1-4 containing ginsenosides Rg1 and Rg2 have a higher melanin production inhibition rate than Comparative Example 5 containing only general red ginseng extract. In particular, ginsenosides Rg1 and Rg2 are It can be seen that the melanin production inhibition rate of the example obtained by mixing each in a weight ratio of 1: 2 is significantly high.

Therefore, a cosmetic for whitening and wrinkle improvement comprising a specific ginsenoside complex in which the peptide complex obtained from the microalgae extract according to the present invention and ginsenosides Rg1 and Rg2 are mixed in a weight ratio of 1 to 3: 1 to 3, respectively It can be seen that the composition has an excellent skin improvement effect.

Although the embodiments of the present invention have been described above, those skilled in the art will understand that the present invention can be implemented in other specific forms without changing the technical spirit or essential features. Therefore, the embodiments described above should be understood as illustrative in all respects and not limiting.

Claims (3)

0.5 to 10.6 parts by weight of peptide complex, 40 to 90 parts by weight of purified water, 0.1 to 5 parts by weight of moisturizer, 0.001 to 10 parts by weight of ceramide, 0.01 to 2 parts by weight of preservative, and 0.001 to 15 parts by weight of ginsenoside complex,
The peptide complex,
A first peptide obtained from a Chlorella vulgaris extract, a second peptide obtained from a Dunaliella salina extract, a third peptide obtained from a Schizochytrium aggregatum extract, Skizokit A fourth peptide obtained from a Schizochytrium limacinum extract, a fifth peptide obtained from a Schizochytrium minutum extract, a sixth peptide obtained from a Crypthecodinium cohnii extract, and Characterized in that the seventh peptide obtained from the Haematococus pluviallis extract was mixed in a weight ratio of 1: 2: 2: 1: 1: 2: 2,
The ginsenoside complex,
Ginsenosides Rg1 and Rg2 are obtained from cultured wild ginseng roots, respectively, and the obtained ginsenosides Rg1 and Rg2 are mixed in a weight ratio of 1 to 3: 1 to 3, respectively. Cosmetic composition for wrinkle improvement using ginsenoside complex obtained from cultured root extract.
delete According to claim 1,
The ginsenoside complex,
Wild ginseng cultured roots are crushed and subjected to primary steaming for 8 hours at a temperature of 30°C and humidity of 40%. The primary steamed cultured wild ginseng cultured roots are placed in a freeze dryer and first dried at -50°C for 10 hours, followed by primary drying. The cultured wild ginseng cultured roots were subjected to secondary steaming for 8 hours at a temperature of 30 ° C and humidity of 40%, and the secondary steamed cultured wild ginseng cultured roots were placed in a freeze dryer and dried a second time at -50 ° C for 10 hours, and then 60 to 120 mesh (mesh) to produce wild ginseng cultured root powder,
An extraction mixture was prepared by adding 1 part by weight of wild ginseng cultured root powder to 100 parts by weight of an aqueous solution of pH 4.8 citric acid, and the extraction mixture was put into a high-pressure reactor for 8 hours, and then the extraction mixture was recovered. From the recovered extraction mixture, ginsenoside Rg1, A cosmetic composition for improving wrinkles using a method for preparing wild ginseng cultured root extract and a ginsenoside complex obtained from wild ginseng cultured root extract, characterized in that Rg2 is obtained and mixed.