KR102463511B1 - Houttuynia cordata Cooling Spicule Shampoo Composition - Google Patents

Abstract

The present invention provides a Houttuynia cordata cooling spicule shampoo composition comprising 5 to 10 wt% of a carboxylate type anionic surfactant, 2 to 10 wt% of an amphoteric surfactant, 0.3 to 3 wt% of at least one cationic polymer selected from dimethyldiallylammonium chloride polymer and dimethyldiallylammonium chloride-acrylic acid copolymer, 0.2 to 2 wt% of an azole antifungal agent, 3 to 8 wt% of spicule, 2 to 5 wt% of menthol, 2 to 8 wt% of a natural extract, and the balance of water. The natural extract includes 2 to 5 parts by weight of a Houttuynia cordata ethanol extract, 2 to 5 parts by weight of a cypress leaf ethanol extract, 2 to 5 parts by weight of a ginger ethanol extract, 2 to 5 parts by weight of an eggplant ethanol extract, 2 to 5 parts by weight of a Dwarf juniper leaf ethanol extract, 2 to 5 parts by weight of an aloe ethanol extract, 2 to 5 parts by weight of a Machilus thunbergii leaf ethanol extract, 2 to 5 parts by weight of an Euscaphis japonica leaf ethanol extract, 2 to 5 parts by weight of a Cheongho ethanol extract, 2 to 5 parts by weight of a mulberry ethanol extract, 2 to 5 parts by weight of a Poncirus ethanol extract, and 2 to 5 parts by weight of a basil ethanol water extract. The present invention is to improve the immunity.

Description

Eoseongcho Cooling Spicule Shampoo Composition {Houttuynia cordata Cooling Spicule Shampoo Composition}

The present invention strengthens the scalp immunity and maintains the easy-to-use formulation of the shampoo, and by composing the ratio of strong antioxidants to the maximum content, strengthens hair follicle cells and induces normal activation of hair regenerative cells to prevent hair loss. it's about

The hair of modern people is contaminated by UV exposure, contact with the surrounding environment such as dust, and sebum secreted from the hair follicles. Scalp diseases such as keratin and dandruff and the resulting hair loss often damage the scalp and hair.

When the homeostasis of the scalp is lowered, the immunity of the scalp is lowered, scalp diseases appear, and the damage to the hair is accelerated due to the deterioration of the function of the regeneration cells of the scalp hair root area. Damaged hair, when wet or dry, is hard to handle due to tangles and stiffness, which makes it difficult to comb, and shows an unsightly appearance due to lack of luster. Accordingly, consumer demand and preference for hair care products (eg, shampoo, conditioner, hair conditioner, hair treatment, etc.) that improve the appearance and feel of hair and make it easier to handle are increasing day by day.

Conventionally, hair care products such as hair conditioner, hair treatment, and hair air conditioner have been used to give moisture or smoothness to hair. In general, anionic surfactants and polymers, silicone conditioning agents, hydrocarbon oils and fats You are using a hair conditioning agent such as alcohol.

Among them, silicone oil is widely used because of its low surface tension, excellent familiarity with hair, and good gloss. However, silicone oil has a problem of causing scalp troubles by clogging the scalp or pores and preventing the smooth secretion of sebum.

There are many types of shampoos, treatments, and hair packs sold in the market, but most of them contain a lot of chemical ingredients such as surfactants and silicone-derived ingredients to temporarily increase the shine and silkiness of hair. However, shampoos, treatments, and hair packs with these ingredients have better cleaning power as they contain a lot of strong alkaline ingredients, but the residue remains on the scalp and hair and penetrates into the body, causing adverse effects on the body, such as the accumulation of transdermal toxins and the generation of carcinogens. also do

Therefore, development of shampoos, treatments and hair pegs containing natural ingredients that have a good effect on hair or scalp is being made. In particular, as the number of hair loss populations increases in recent years, studies on shampoos having a hair loss prevention and hair growth function are being actively conducted. If the ratio of plant-derived surfactants, weakly acidified ingredients, and natural active ingredients is increased as much as possible, the formulation of the shampoo becomes unstable, resulting in an uncomfortable feeling of use. Shampoos made with a limited fragrance such as an uncomfortable feeling of use for scalp health or herbal medicines are also available on the market, but consumers are also limited in their consumption due to the uncomfortable feeling of use compared to the efficacy.

Republic of Korea Patent Publication No. 2014-0086673

The present invention has been devised to solve the above disadvantages of the prior art,

An object of the present invention is to provide a cooling spicule shampoo composition for hair loss prevention by strengthening scalp homeostasis and immunity, strengthening hair follicle cells with the maximum content ratio of strong antioxidant components, and normalizing and activating hair regeneration cell division.

In order to achieve the above object, the present invention

At least one cationic polymer selected from 5 to 10% by weight of carboxylate-type anionic surfactant, 2 to 10% by weight of amphoteric surfactant, dimethyldiallylammonium chloride polymer and dimethyldiallylammonium chloride-acrylic acid copolymer 0.3 to 3% by weight, 0.2 to 2% by weight of an azole antifungal agent, 3 to 8% by weight of spicule, 2 to 5% by weight of menthol, 2 to 8% by weight of a natural extract, and the balance of water,

The natural extract is eoseongcho ethanol extract 2 to 5 parts by weight, cypress leaf ethanol extract 2 to 5 parts by weight, ginger ethanol extract 2 to 5 parts by weight, eggplant ethanol extract 2 to 5 parts by weight, oleifera leaf ethanol extract 2 to 5 parts by weight Part, 2 to 5 parts by weight of aloe ethanol extract, 2 to 5 parts by weight of ethanol extract of sagebrush leaves, 2 to 5 parts by weight of ethanol extract of Eucapphis japonica leaf, 2 to 5 parts by weight of ethanol extract of Cheongho, ethanol extract of mulberry 2 to 5 parts by weight, 2 to 5 parts by weight of ethanol extract, and 2 to 5 parts by weight of basil ethanol water extract It provides a cooling spicule shampoo composition comprising eoseongcho.

The Eoseongcho cooling spicule shampoo composition of the present invention contains ingredients extracted from various kinds of natural products, thereby strengthening the hair follicle cells and promoting normalization and activation of hair follicle cell division and prevention of hair loss with strong antioxidant ingredients and excellent scalp homeostasis and immunity. function is provided.

Hereinafter, the present invention will be described in detail.

Eoseongcho cooling spicule shampoo composition of the present invention contains 5 to 10% by weight of a carboxylate-type anionic surfactant, 2 to 10% by weight of an amphoteric surfactant, a dimethyldiallylammonium chloride polymer and a dimethyldiallylammonium chloride-acrylic acid copolymer. 0.3 to 3% by weight of at least one cationic polymer selected from, 0.2 to 2% by weight of an azole antifungal agent, 3 to 8% by weight of spicule, 2 to 5% by weight of menthol, 2 to 8% by weight of natural extract, and the remainder contains water of

The natural extract is eoseongcho ethanol extract 2 to 5 parts by weight, cypress leaf ethanol extract 2 to 5 parts by weight, ginger ethanol extract 2 to 5 parts by weight, eggplant ethanol extract 2 to 5 parts by weight, oleifera leaf ethanol extract 2 to 5 parts by weight Part, 2 to 5 parts by weight of aloe ethanol extract, 2 to 5 parts by weight of ethanol extract of sagebrush leaves, 2 to 5 parts by weight of ethanol extract of Eucapphis japonica leaf, 2 to 5 parts by weight of ethanol extract of Cheongho, ethanol extract of mulberry It is characterized in that it contains 2 to 5 parts by weight, 2 to 5 parts by weight of an ethanol extract of oleic acid, and 2 to 5 parts by weight of an ethanol water extract of basil.

The carboxylate-type anionic surfactant may be included in order to improve the dandruff and itch improvement effect. The component is not particularly limited, and examples thereof include an ether carboxylic acid type surfactant and an amino acid type surfactant. These may be used individually by 1 type, and may use 2 or more types together. Among these, ethercarboxylic acid type surfactant is preferable.

As said ether carboxylic acid type surfactant, polyoxyethylene lauryl ether sodium acetate, laureth-4 carboxylic acid, sodium lauryl glycol carboxylate etc. are mentioned, for example.

As the amino acid-type surfactant, for example, N-acyl sarcosine salt, N-acyl alanine salt, N-acyl glutamate, N- acyl aspartate, etc. may be preferably used in terms of dandruff and itch improvement effects.

As the amphoteric surfactant, for example, an alkylbetaine-type amphoteric surfactant, an amidebetaine-type amphoteric surfactant, an imidazolinium betaine-type amphoteric surfactant, etc. are preferable in terms of dandruff and itch improvement effects, and lauryldimethylamino Acetate betaine, lauric acid amide propyl betaine, coconut oil fatty acid amide propyl betaine, 2-alkyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine, etc. are more preferable.

In the at least one cationic polymer selected from the dimethyldiallylammonium chloride polymer and the dimethyldiallylammonium chloride-acrylic acid copolymer, the dimethyldiallylammonium chloride-acrylic acid copolymer is a structural unit derived from dimethyldiallylammonium chloride. The molar ratio of is 65 mol% or more, and is represented by the following general formula (C1).

In the general formula (C1), n and m represent the molar ratio (mol%) of each structural unit, n+m=100, and m is 65 mol% or more.

The molar ratio of the structural unit derived from dimethyldiallylammonium chloride in the dimethyldiallylammonium chloride-acrylic acid copolymer is 65 mol% or more, preferably 95 mol% or more.

If the molar ratio is less than 65 mol%, the effect of improving dandruff and itching cannot be realized. The said dimethyldiallylammonium chloride-acrylic acid copolymer may be used individually by 1 type, and may use 2 or more types together.

10,000-1,000,000 are preferable and, as for the weight average molecular weight of the said cationic polymer, 15,000-450,000 are more preferable.

The azole antifungal agent may be included in order to improve the effect of improving dandruff and itching. The azole antifungal agent includes, for example, miconazole, clotrimazole, econazole, thioconazole, oxyconazole, biponazole, lanoconazole, luliconazole, isoconazole, ketoconazole, fluconazole, itraconazole, netico and nasol, sulconazole, phosfluconazole, voriconazole, croconazole, or salts thereof. These may be used individually by 1 type, and may use 2 or more types together. The salt thereof may be, for example, nitrate or hydrochloride.

As said azole antifungal agent, miconazole and/or its salt are preferable from the point of dandruff and itch improvement effect, and miconazole nitrate is more preferable.

The spicule is also called a sponge, and may be extracted from a sponge. Sponges are primitive marine organisms that do not have muscles, nerves, or organs. There are more than 5,000 species and they are found regardless of the depth of the sea. The bone fragments of sponges are needle-shaped tissues that exist in the body of invertebrates and are mainly composed of silicate and calcium carbonate. The fibrous skeleton of sponges has the ability to absorb according to the capillary phenomenon, so it is being applied in various fields such as medicine. Spongy bone fragments are divided into sponge bone fragments containing antibiotics for the treatment of osteomyelitis, treatment derived from sponges for the treatment and prevention of skin diseases, and the use of sponge bone fragments as a skin polishing device or skin regenerating means. These spicules have a substantially needle-like shape and have porous properties, so that they can effectively contain active ingredients to increase the functionality of the shampoo composition.

The spicule is also called hydrolyzed sponge (Hydrolyzed Sponge), and may be prepared by a chemical purification process using a generally known acid or base, obtained by adding a proteolytic enzyme such as protease to the sponge powder It can also be prepared by digesting a single protein. These spicules are sold in powder form in the market, and those having a particle size in the range of 50 to 100 μm may be used.

The natural extract is selected to provide an excellent scalp homeostasis and immunity enhancement in the shampoo composition, and is a strong antioxidant component that strengthens hair follicle cells, induces normalization and activation of hair regeneration cell division, and provides a hair loss prevention function. It is an ethanol extract made from a natural product.

In the natural extract, the Houttuynia cordata is hepatitis, cardiopulmonary agent, gaecal, expectorant, high blood pressure, coronary artery disease, arthritis, bronchitis, cold disease, hypothyroidism, arteriosclerosis, syphilis, athlete's foot, cystitis, edema, postpartum edema, edema, sore throat , cancer (esophageal cancer), laxativeness, urethritis, edema, silver dust, diuresis, dysentery, sore throat, sore throat, endometritis, endometritis, swelling, intoxication, otitis media, paralysis, sinusitis, fistula, tooth ulcer, prolapse, lung abscess, pneumonia, skin beautification (Rough skin - when you want a smooth texture), dermatitis, fever, and suppuration.

The cypress leaf is one of the representative species known to produce phytoncide, which itself generates anions, and is a material containing a large amount of vitamins and minerals beneficial to the human body. Phytoncide gives psychological stability, strengthens peripheral blood vessels, strengthens cardiopulmonary function, helps bronchial asthma and pulmonary tuberculosis, and exhibits pharmacological action to disinfect skin.

The ginger (ginger, gingembre) is slightly warm in nature, very tasty, and non-toxic. It enters the five intestines, removes walls, lowers qi, and stops vomiting. It also removes wind and dampness, and cures hiccups, bloating, shortness of breath, and coughing.

The snow juniper (Juniperus chinensis var. sargentii) is an evergreen coniferous shrub of the Arboraceae family. It is also called a cypress tree, a cypress tree, and a spruce tree. It grows in crevices of rocks in high mountains. The main stem stands obliquely or extends to the ground. It is similar to juniper, but grows sideways and has serpentine branches. The leaves are sharp needles when they are young, but they do not sting like cypress trees. When they get old, they become only scaly leaves. There are two white lines wider than veins, and the back side is bluish green. The scaly leaves are alternately opposite to each other on small branches and have a rhombic shape with a round tip. Flowers bloom in May and are single male and female. Male flowers are egg-shaped, 2mm long, and female flowers are ball-shaped and hang at the tip of branches. The fruits are conifers and ripen in October of the following year. It is bluish, spherical, and contains 1 to 3 egg-shaped seeds. It is mainly planted for ornamental purposes, and is distributed in Korea, Japan, Taiwan, and Sakhalin Island.

The Machilus thunbergii is an evergreen broad-leaved arboreous tree belonging to the Lauracecae family, and inhabits warm regions such as Korea, southern China, Japan, and Taiwan. It has been reported that the leaves of the spruce tree contain flavonoids such as quercetin, afzelin, rutin and trifolin.

In the case of the horse pee (Euscaphis japonica), it is a deciduous broad-leaved small arboreous tree distributed in Japan, China, and Taiwan as its origin in Korea. It is often grown in the mountains of the southern region and is weak to cold, and wintering is impossible in the central region. At the time of urination, yellow-green flowers are gathered in panicles at the ends of branches, and calyxes are arranged in a tile-like arrangement, and the fruits are enlarged and have the shape of a bud. The roots of horse urine are called ya-chun-geun, and the fruit is called ya-chun-ja, so it is said to be used for all kinds, ginseng, small bell, nausea, stomach pain, dryness, desulfurization, dysmenorrhea, dysentery, colic, rheumatism, etc. known to be effective.

The "Chungho (Artemisiae Annuae Herba)" refers to the above-ground part of the dicotyledonous plant, Artemisiaannua Linne or Artemisia apiacea Hance. Mugwort wormwood is also called mugwort mugwort, mugwort mugwort, chogo, hyanggo, wormwood, and wormwood, and is used in oriental medicine to treat fever, cold, dysentery, pediatric dysentery, indigestion, dysentery, etc. In addition,

The mulberry is a fruit of a mulberry tree, also called mulberry, and the skin is a little chewy, but the shape is similar to a strawberry, so it is better to use it immediately when it is ripe black. It begins to bear light green fruits in April or May and ripens to black purple in June. Like mulberry leaves, mulberry contains plenty of minerals. In addition to calcium, potassium, iron, and magnesium are contained in large amounts compared to other fruits.

The branch is a fruit of Citrus aurantium L. Poncirus trifoliata Rafin., an evergreen small tree belonging to the Rutaceae family, and immature fruits are collected in May and June, cut horizontally, or dry at low temperature. Ingredients of oilseed oil include essential oils such as limonene, linalool, camphene, etc., and pravos such as poncirin, naringin, hesperidin, neohesperidin, etc. Coumarins such as noid and umbelliferone, auraptene, and imperatorin have been reported. Ponkyrin is known to have anti-platelet action, intestinal bacteria suppression action, and anti-helicobacter pylori activity, and naringin has been reported to have cholesterol suppressive effect, change leukemia cells into normal cells, and inhibit breast cancer cell proliferation.

The shampoo composition may further include 2 to 5% by weight of the Arctic Stork extract. The Arctic Stork extract performs the function of further improving the antibacterial performance of the shampoo composition. The extract may be preferably extracted with a mixed solvent of ethanol and butylene glycol, and the mixing ratio may be 4:6 to 2:8 by weight.

Arctic stork grass is a grass commonly found in Arctic tundra wetlands, and has a characteristic that the seeds look like cotton wool. The extract extracted from white cotton, which is a seed, may be more preferably used as the Arctic Stork extract, but a component extracted from the stem may also be used.

When the Arctic Stork extract is included in the shampoo composition together with the above ingredients, it was found that antioxidant and hair loss prevention effects were also increased.

Arctic stork grass extract according to the present invention is extracted with a solvent mixed with ethanol and butylene glycol in a weight ratio of 1:9 to 5:5, preferably 2:8 to 4:6, of white cotton, a dry seed. do. The extraction may be carried out at 50 to 60 ℃.

After obtaining the extract extracted from the Arctic Stork, it is filtered, concentrated, and dried to obtain an Arctic Stork extract.

The shampoo composition may further include 0.1 to 1.5% by weight of fragrance oil.

The fragrance oils include Peppermint E.O, Rosemary E.O, Ylang Ylang E.O, Cedarwood E.O, Geranium E.O, Lavender E.O, Lemon E.O, Orange E.O, Citronella E.O, Eucalyptus E.O, Frankincense E.O, Tea Tree E.O, Melissa E.O, At least one selected from Lemongrass E.O, Pine E.O, Lilac F.O, Doson F.O, Bay F.O, Cherry F.O, Jasmine F.O, and Nenoli F.O may be used.

Components used in the shampoo composition of the present invention that are not described in detail above are components commonly used in this field. Therefore, it can be easily used by purchasing it from the market.

Hereinafter, the present invention will be described in more detail through examples. However, the following examples are provided to explain the present invention in more detail, and the scope of the present invention is not limited by the following examples. The following examples can be appropriately modified and changed by those skilled in the art within the scope of the present invention.

Preparation Example 1: Preparation of natural extract

300g each of dried eoseongcho, cypress leaf, ginger, eggplant, juniper leaf, aloe, sagebrush leaf, Eucapphis japonica leaf, cheongho, mulberry, oilseed fruit, and basil in an extraction container, and 5 times Ethanol (70% concentration) was added (by weight) and extracted while stirring at 50 to 60° C. for 4 hours. The extract was filtered under reduced pressure with a vacuum pump and Whatman No. 2 filter paper, and the filtered extract was concentrated with a vacuum rotary concentrator and dried to obtain a natural extract in powder form.

Preparation Example 2: Preparation of Arctic stork grass extract

After shredding 1 kg of dried Arctic Stork cotton, put it in 2L of a mixed solvent of ethanol (concentration 40% (v/v)) and butylene glycol (weight ratio 3:7), and at 50 to 60° C. for 4 hours It was extracted while stirring. The extract was filtered under reduced pressure with a vacuum pump and Whatman No. 2 filter paper, and the filtered extract was concentrated with a vacuum rotary concentrator and then spray-dried to obtain an extract of Arctic Stork in powder form.

Experimental Example 1: Safety evaluation

The stability of the extracts prepared in Preparation Examples 1 and 2 was tested through a 48-hour patch test using Guinea Pig. The degree of irritation was evaluated and the results are shown in Table 1 below.

<Evaluation criteria>

○: Non-irritating (there is almost no erythema and edema)

△: mild irritation (very mild erythema and edema)

×: moderate irritation (moderate edema and obvious erythema)

Properties Preparation Example 1 Preparation 2 pepper ○ ○

As shown in Table 1, the extracts of Preparation Examples 1 and 2 were confirmed to be safe for the skin.

Test Example 2: Measurement of antibacterial activity of Arctic stork grass

The antibacterial activity of the Arctic Stork extract (Preparation Example 2) contained in the shampoo composition of the present invention was evaluated as follows.

Representative food poisoning bacteria (Bacillus cereus, Bacillus subtilis, Campylobacter jejuni, Escherichia coli, Escherichia coli O-157, Listeria monocytogenes, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella enteritidis, Salmonella thypimurium, Vibrio parahaemolyticus) were used.

The bacteria were inoculated with 50 μl of the culture in 10 ml of sterile TSB medium and subcultured at 30° C. for 24±1 hours. The concentration of this culture was maintained at an Abs 0.6±0.1 at an absorption wavelength of 655 nm in a microplate reader. The subcultured test bacteria were diluted 10 ^-3 times with sterile distilled water, and the disc diffusion method was performed. That is, the diluted strain was put on a sterile cotton swab and spread on a 4 mm thick plate medium, and a sterilized 8 mm (thickness 1 mm) paper was raised and 50 μl of the Arctic Stork extract (Preparation Example 2) was injected, respectively. After sufficient inhalation for about 20 minutes, culture was performed at 30°C for 24±1 hours, and growth rings were confirmed.

The antibacterial activity was evaluated by measuring the size of the translucency including the filter paper, and the results are shown in Table 2 below.

strain name strain number ^a Antibacterial activity (mm) ^b Arctic Stork Extract bacteria Bacillus cereus KCTC 1012 16 Bacillus subtilis KCCM 1021 17 Campylobacter jejuni KCCM 41772 16 Escherichia coli ATCC 25922 17 Escherichia coli O-157 ATCC 43888 16 Listeria monocytogenes ATCC 15313 16 Pseudomonas aeruginosa KCTC 1750 17 Staphylococcus aureus ATCC 25923 16 Salmonella enteritidis KCCM 11862 16 Salmonella thypimurium ATCC 13076 17 Vibrio parahaemolyticus ATCC 17802 17 Yersinia enterocolitica ATCC 23715 17

Co., Ltd. ^a : ATCC American Type Culture Collection, KCTC Korea Research Institute of Bioscience and Biotechnology Biological Resources Center; KCCM Korea Sprout Association

^b : size of transparencies (diameter mm)

As can be seen in Table 1, the extract of Arctic Stork contained in the shampoo composition of the present invention (prepared in Preparation Example 2) exhibited very excellent antibacterial activity against all bacteria.

Example 1: Preparation of shampoo composition

A shampoo composition was prepared by mixing the ingredients of Table 3 below.

Ingredients Content (wt%) Carbonate type anionic surfactant
(Polyoxyethylene lauryl ether sodium acetate 8 amphoteric surfactant
(Lauric acid amide propyl betaine) 7 Cationic polymer dimethyldiallylammonium chloride-acrylic acid amide copolymer (molar ratio 95:15) 2 Azole-based antifungal drugs (miconazole nitrate) One spicule 6 menthol 4 Natural extracts 5 fragrance oil 0.5 water remaining amount

Example 2: Preparation of shampoo composition

A shampoo composition was prepared in the same manner as in Example 1, except that 4% by weight of the Arctic Stork extract prepared in Example 1 was further added and 4% by weight of water was reduced.

Comparative Example 1.

Head and Shoulders Itchy Scalp Total Solution Shampoo, which is a commercially available weak acid hair loss shampoo, was used as Comparative Example 1.

Experimental Example 3: Evaluation of scalp immunity improvement effect

1) Subject selection

Fifteen women aged 20-59 years with damaged hair and having a sensitive scalp with a scalp pH index of 5.6 or higher, scalp redness, itching, dandruff, and dead skin cells were selected as subjects.

2) Test method

After wetting the scalp and hair with lukewarm water, apply 3 ml to 10 ml of the shampoo of Example 1 (5 people), Example 2 (5 people) and Comparative Example 1 (5 people) to the hands and wash the hands Make bubbles by rubbing. Next, the shampoo with foam was applied to the scalp and hair, and then washed with lukewarm water.

3) Test result

(1) Scalp pH measurement

In order to check the change in scalp pH, the scalp pH was measured before, immediately after, and after 2 weeks of use of the shampoo, and the results of 5 people in each group were averaged and shown in Table 4 below.

Scalp pH (Mean±SD) Example 1 Example 2 Comparative Example 1 before use 5.72±0.21 5.81±0.15 5.79±0.11 immediately after use 5.55±0.11 5.42±0.16 5.72±0.13 2 weeks after use 5.52±0.13 5.37±0.11 5.72±0.15

* : P<0.05 by repeated measure ANOVA, post hoc Bonferroni correction Normal hair and scalp pH is 4.5~5.5, slightly acidic, and the possibility of weak acidity protects itself from harmful environments such as external bacteria, bacteria, dust, rapid temperature and humidity changes, and strong UV rays. Maintains protective homeostasis. Therefore, a shampoo that can lower the pH of the scalp as in the present name can contribute to maintaining homeostasis.

(2) Scalp keratin measurement

In order to confirm the change in scalp keratin, scalp keratin was measured before, immediately after, and after 2 weeks of use of the shampoo, and the results of 5 people in each group were averaged and shown in Table 5 below.

Scalp keratin (Mean±SD) Example 1 Example 2 Comparative Example 1 before use 14.65±3.58 14.98±2.35 14.90±3.65 immediately after use 11.32±2.51 9.8±2.52 12.78±211 2 weeks after use 8.31±2.21 7.44±2.11 12.12±3.21

* : P<0.05 by repeated measure ANOVA, post hoc Bonferroni correction

Experimental Example 4: Measurement of antioxidant activity

(1) Analysis of total polyphenol content

Examples 1, 2 and Comparative Example 1 To measure the antioxidant activity of shampoos, 50 μl of folin-ciocalteu reagent was added to 0.1 ml of each shampoo, mixed, and reacted for 4 minutes, followed by 1.5 ml of 20% saturated anhydrous sodium carbonate solution was added. After reaction for 2 minutes, absorbance was measured at 760 nm using a microplate reader (VERSAmax, Molecular Device, CA, USA). The total polyphenol content was calculated from a standard curve prepared using chlorogenic acid.

(2) DPPH radical scavenging activity measurement

160 μl of DPPH solution dissolved in methanol to a concentration of 0.4 mM and 40 μl of each shampoo sample were added, left in the dark for 30 minutes, and then absorbance was measured at 515 nm using a microplate reader (VERSAmax, Molecular Device, CA, USA). .

Example 1 Example 2 Comparative Example 1 Total polyphenol mg/ml 56.8 63.1 42.3 DPPH R S (%) 76 83 62.8

Experimental Example 5: Evaluation of hair loss prevention effect

Examples 1, 2 and Comparative Example 1 In order to confirm the hair loss prevention effect of the shampoo, 5 out of 15 male test subjects with alopecia were used in units of Example 1, Example 2 and Comparative Example 1 shampoo 3 The hair was washed once a day for months, and the results of use were evaluated and shown in Table 7.

<Evaluation criteria>

The amount of hair loss at the time of hair washing, subjective symptoms, and visual judgment were evaluated as 5 points if the effect was significant, 4 points if moderate, 3 points if mild, 2 points if unchanged, and 1 point if hair loss symptoms worsened. Comprehensive judgment was made as an average value.

Example 1 Example 2 Comparative Example 1 Amount of hair loss when washing hair 4.2 4.7 3.7 subjective symptoms 4.3 4.8 3.5 Visual judgment 4.2 4.7 3.4 Synthesis 4.23 4.73 3.53

Claims (4)

5 to 10% by weight of an anionic surfactant of the carboxylate type, 2 to 10% by weight of an amphoteric surfactant, 0.3 to 3% by weight of a cationic polymer that is a dimethyldiallylammonium chloride-acrylic acid copolymer, 0.2 to 2% by weight of an azole antifungal agent , 3 to 8% by weight of spicule, 2 to 5% by weight of menthol, 2 to 8% by weight of natural extract, and the balance of water,
The natural extract is eoseongcho ethanol extract 2 to 5 parts by weight, cypress leaf ethanol extract 2 to 5 parts by weight, ginger ethanol extract 2 to 5 parts by weight, eggplant ethanol extract 2 to 5 parts by weight, oleifera leaf ethanol extract 2 to 5 parts by weight Part, 2 to 5 parts by weight of aloe ethanol extract, 2 to 5 parts by weight of ethanol extract of sagebrush leaves, 2 to 5 parts by weight of ethanol extract of Eucapphis japonica leaf, 2 to 5 parts by weight of ethanol extract of Cheongho, ethanol extract of mulberry 2 to 5 parts by weight, 2 to 5 parts by weight of ethanol extract, and 2 to 5 parts by weight of basil ethanol water extract As a eosincho cooling spicule shampoo composition comprising:
The shampoo composition further comprises 2 to 5% by weight of the Arctic Stork Som extract extracted with a solvent mixed with ethanol and butylene glycol in a weight ratio of 2:8 to 4:6,
The shampoo composition further comprises 0.1 to 1.5% by weight of fragrance oil,
The fragrance oils are Peppermint EO, Rosemary EO, Ylang Ylang EO, Cedarwood EO, Geranium EO, Lavender EO, Lemon EO, Orange EO, Citronella EO, Eucalyptus EO, Frankincense EO, Tea Tree EO, Melissa EO, Lemongrass EO, Pine EO, Lilac FO, Dawson FO, Bay FO, Cherry FO, Jasmine FO, and Nenoli FO, each containing 0.02 to 0.06% by weight of Eoseongcho cooling spicule shampoo composition. delete delete delete