KR102437393B1 - Manufacturing method of growth promoters for livestock - Google Patents

Abstract

Disclosed content relates to a method for producing a growth promoter for livestock that can increase the amount of calcium absorbed into the body of livestock and produce a growth promoter for livestock that increases the bioavailability of calcium, and reduces the manufacturing cost of the growth promoter for livestock .
The disclosed contents are a first step of preparing a hydrolyzate of yeast by mixing dry inert yeast, process water, and an enzyme, a second step of mixing inorganic calcium, a peptide source, and process water with the hydrolyzate of the yeast, the second step A third step of drying the mixture made through A method of manufacturing a growth promoter for livestock is presented as an example.

Description

Manufacturing method of growth promoters for livestock {Manufacturing method of growth promoters for livestock}

The disclosed subject matter relates to a method of manufacturing a growth promoter for livestock to aid livestock feed.

Unless otherwise indicated herein, the contents described in this identification are not prior art to the claims of this application, and the description in this identification is not admitted to be prior art.

Yeast has been used as a raw material for various useful substances in the food field such as brewing and baking industry for a long time, and contains about 50% of nucleic acids such as proteins, lipids, RNA, and various vitamins and minerals in the cells. Yeast extract produced from yeast is used as a microbial fermentation medium and raw material for high-grade and health-oriented seasonings. It is a raw material that can be used as a substitute for or combined use of acid-decomposed HVP (hydroyzed vegetable protein), and is also used as a substitute for MSG (Manjin In, Heejeong Chae, etc.) , 2004)

The production and application of yeast extracts have been continuously studied and reported. (Hirosh, 1974, Izzo and Ho, 1992, Choi and Chung 1998)) The production of yeast extracts is largely divided into self-extinguishing methods and enzymatic digestion methods. is an economical yeast extract manufacturing method in which salt or ethanol is added to a 10-20% baker's yeast suspension and stirred at 30-70°C to obtain a decomposition product that is self-decomposed by the action of enzymes present in yeast cells. The enzymatic digestion method basically sequentially adds a cell wall lysing element, a proteolytic enzyme, a nucleolytic enzyme, and the like, and the yield is improved compared to the self-digestion method, but there is a disadvantage in economic terms due to the use of an additional enzyme. Various studies on the feeding of yeast extract to food and feed have been steadily reported. In particular, it is mentioned that many kinds of peptides and amino acids contained in yeast extract have anti-stress effects (Kim, 2003).

On the other hand, calcium is an essential mineral nutrient that is involved in many biological processes, including cell signaling, nerve conduction, enzyme activity regulation, muscle contraction, and skeletal tissue (Carafoli, 2002, Miller & McBean, 2001, Zhou & Yang, 2013). The action of calcium in the body is very wide. Life-sustaining and skeletal metabolic functions Calcium exists in body fluids including bones, teeth and blood, and performs major life-sustaining and skeletal metabolic functions, such as heartbeat, nerve transmission, blood coagulation, and regulation of movement of substances through cell membranes. To maintain these functions, it is essential to maintain the homeostasis of calcium concentration in cells and blood. The difference in concentration is very large. When the calcium concentration in the blood is 1, the calcium concentration in the cell must be maintained at 1/10,000. Only by maintaining such a large concentration difference, calcium can normally transmit information to cells throughout the body. When calcium is insufficient, the balance between the concentration difference between cells and blood is broken, and cells that do not receive normal information are the cause of various diseases (Conigrave & Brown, 2008).

As an example of a method of manufacturing such a feed for livestock containing calcium, Korean Patent Publication No. 10-2015-0113318 (published on Oct. 8, 2015) discloses that chitin is prepared using crab shells, and the general After analyzing the ingredients and chitin content, a supplementary feed for livestock containing the chitin prepared above was prepared and fed to Korean cattle to evaluate the grade and meat quality of Korean beef. By adding chitin and chitosan to livestock supplements and feeding them to livestock, the quality of meat is improved, which not only improves the value of livestock meat in terms of nutrition, functionality, and quality, but also contains calcium that can maximize the use of crab shells. Disclosed is a method for preparing an auxiliary feed for livestock containing a mixture of chitin and chitosan as an active ingredient.

As another example, Korean Patent Laid-Open No. 10-2005-0065856 (published on June 30, 2005) discloses the process of washing shells, drying/sterilizing them, grinding them to make shells or preparing limestone, and manufacturing green tea beverages. After properly pulverizing the discarded green tea leaves, mixing and kneading the powder or limestone prepared above through a binder as a medium to granulate them to a diameter of 1-4 mm, and drying the granulated auxiliary feed with hot air to form a pellet. In addition, after washing the shells, drying/sterilizing them, grinding them to make shells or preparing limestone, and coating the concentrate of green tea leaves on the shells or limestone granules or pulverized products prepared above The process of adding or adding granulated auxiliary feed is disclosed by drying the granulated auxiliary feed with hot air to prepare a pellet shape.

Although the supplementary feed prepared by the method for producing an auxiliary feed for livestock contains calcium, all of the calcium of these feed additives is difficult to be absorbed into the body of the livestock, and there is a problem in that the bioavailability of calcium is low. In addition, the prior literature does not specifically disclose a method for treating heat and by-products generated during the auxiliary feed manufacturing process.

Patent Document 1: Republic of Korea Patent Publication No. 10-2015-0113318 (published on Oct. 8, 2015) Patent Document 2: Korean Patent Publication No. 10-2005-0065856 (published on June 30, 2005)

The disclosed invention is to increase the amount of calcium absorbed into the body of livestock and to produce a growth promoter for livestock that increases the bioavailability of calcium, and to provide a method for producing a growth promoter for livestock that can reduce the manufacturing cost of the growth promoter for livestock do.

In addition, it is not limited to the technical problems as described above, and it is obvious that another technical problem may be derived from the following description.

The disclosed contents include a first step of preparing a yeast hydrolyzate by mixing inactive yeast, process water, and an enzyme, a second step of mixing inorganic calcium, a peptide source, and process water with the yeast hydrolyzate, the second step A third step of drying the mixture made through a third step, a fourth step of pulverizing the dry mixture, and a fifth step of completing the growth promoter for livestock by mixing an excipient, a dispersing agent, and a stabilizer with the pulverized pulverized product A method of manufacturing a growth promoter for livestock is presented as an example.

According to the characteristics of the disclosed contents, the sixth step, which is a quality analysis step of checking the binding of calcium and peptide of the completed growth promoter for livestock, properties, and moisture content, and packaging and storing the growth promoter for livestock analyzed for the quality It may further include a seventh step.

According to the method for producing a growth promoter for livestock according to an embodiment of the disclosed disclosure, there is an advantage in that it is possible to manufacture a growth promoter for livestock that increases the amount of calcium absorbed into the body of livestock and increases the bioavailability of calcium. In addition, there is an advantage that can reduce the manufacturing cost of the growth promoter for livestock.

The effects of the present invention are not limited to the above-mentioned effects, and other effects not mentioned will be clearly understood by those skilled in the art from the description of the claims.

1 is a flowchart of a method for manufacturing a growth promoter for livestock according to an embodiment of the disclosed subject matter.
Figure 2 is a flowchart of a method of manufacturing a growth promoter for livestock according to another embodiment of the disclosed subject matter.

Hereinafter, with reference to the accompanying drawings, the configuration and effects of preferred embodiments of the disclosed contents will be described. For reference, in the following drawings, each component is omitted or schematically illustrated for convenience and clarity, and the size of each component does not reflect the actual size. In addition, the same reference numerals refer to the same components throughout the specification, and reference numerals for the same components in individual drawings will be omitted.

As shown in FIG. 1, the method of manufacturing a growth promoter for livestock according to an embodiment of the disclosed content includes a first step (S1) of preparing a hydrolyzate of yeast, a second step (S2) of mixing the raw materials, A third step (S3) of drying the mixture that has undergone the above steps, a fourth step (S4) of pulverizing the dried mixture, and a fifth step (S5) of mixing an excipient and the like with the pulverized product are included.

The first step (S1) is a step of preparing a hydrolyzate of yeast, which is one of the raw materials of feed additives for livestock. Yeast hydrolyzate is a product obtained by hydrolyzing yeast, and is prepared by mixing dry inert yeast, process water, and enzymes. At this time, 1 to 4 parts by weight of the dry inert yeast, 3 to 24 parts by weight of process water, and 0.001 to 0.005 parts by weight of the enzyme are mixed. When the dry inert yeast is 20 kg, the process water is 100 kg, and the enzyme is 20 g, it is preferable to mix the raw materials at 40° C. for 4 hours.

In terms of temperature conditions, it is preferable to set it within the range of 40°C to 70°C. If mixing proceeds at a temperature higher than 70° C., yeast and enzymes mainly composed of protein cannot function in the disclosed growth promoter for livestock due to protein modification. Conversely, if the mixing proceeds at a temperature lower than 40° C., heat generated while hydrolyzing the yeast may be unnecessarily controlled, resulting in a problem of energy wastage or increased manufacturing cost. Considering this point, it is preferable to mix the amount of the raw material at 40°C.

In terms of time conditions, it is preferable to mix the above raw materials for 3 to 8 hours. If the raw materials are mixed in less than 3 hours, the raw materials are not sufficiently mixed and the yeast hydrolyzate is not sufficiently produced, and the gas generated during the yeast hydrolysis cannot be sufficiently discharged. Conversely, mixing raw materials for more than 8 hours wastes manufacturing time and energy. Considering this point, it is preferable to mix for 4 hours with respect to the amount of the raw material.

The enzyme refers to a proteolytic enzyme, and examples thereof include bromelain or papain.

The second step (S2) is a step of mixing the hydrolyzate of yeast prepared in the first step (S1), inorganic calcium, a peptide source, and process water. The second step (S2) is a process of binding calcium ions derived from the peptide source and inorganic calcium. Through this process, the growth promoter for livestock prepared from the disclosed method for producing a growth promoter for livestock can increase the amount of calcium absorption in the body of livestock. In the second step (S2), an excipient may be additionally mixed with the hydrolyzate of yeast in addition to inorganic calcium, a peptide source, and process water.

The yeast hydrolyzate, inorganic calcium, peptide source, process water, and excipients should be mixed in an appropriate ratio, and when the hydrolyzate of yeast is 140.02 kg, it is preferable to mix the raw material for 1 hour and then mature for 1 day. .

In the mixing time, if it is less than 30 minutes, mixing does not proceed sufficiently, so that the peptide source and calcium ions do not sufficiently bind. Conversely, more than 2 hours can waste energy and time. In consideration of this point, the hydrolyzate of yeast, inorganic calcium, peptide source, process water, and excipients are preferably mixed for 1 hour.

Aging of the mixed mixture is to ensure that the interaction between the above raw materials is sufficiently achieved so that the disclosed growth promoter for livestock can exert its efficacy better. In the aging period, 0.5 to 4 days are preferable. If the aging period is less than 0.5 days, the ripening period is short, so that the interaction between the raw materials is not sufficiently performed. Conversely, if the aging period exceeds 4 days, the manufacturing time of the disclosed growth promoter for livestock becomes long, which may cause inefficiency and problems in that raw materials may be damaged.

The third step (S3) is a step of drying the mixture made through the second step (S2). The third step (S3) is a process of reducing the moisture content of the mixture. At this time, the moisture content of the mixture is preferably dried to a level of 5 to 12%. If the water content of the mixture is less than 5%, it may not be easy for livestock to consume. Conversely, if the moisture content of the mixture exceeds 12%, it is entangled with the raw materials added later, and a normal product cannot be made.

The fourth step (S4) is a step of pulverizing the dry mixture. The grinding tool may include a hammer mill, a rod mill, a ball mill, a raymond mill, a jet mill, and the like. Among them, it is preferable to use a hammer mill, a cutter mill, a pin mill, or the like. In particular, it is preferable to use a hammer mill in which a disk on which a plurality of blades are disposed is subjected to shear impact grinding while rotating at a high speed. The pulverized mixture has a particle diameter of 0.1 to 3 mm, and the particle can pass through a 3 mm screen mesh. If the particle diameter of the mixture exceeds 3 mm, it may not be easy for livestock to consume, and it may not be sufficiently mixed with additionally added raw materials.

The fifth step (S5) is a step of mixing an excipient, a dispersing agent, and a stabilizer with the pulverized product. The fifth step (S5) is the final step in which the disclosed growth promoter for livestock is completed. If the excipient is additionally mixed in the second step (S2), it is not necessary to add the excipient in the fifth step (S5). The excipient serves to provide a protein, amino acid, or peptide capable of additionally binding to calcium ions derived from the inorganic calcium. Such materials include soybean meal, fish meal, meat bone meal, resin meal, and rapeseed meal. The dispersant enables the disclosed growth promoter for livestock to have the effect of promoting growth and strengthening immunity of livestock. A material that can be used as a dispersant is gelite. The stabilizer serves to prevent deterioration of the disclosed growth promoter for livestock.

Although the disclosed growth promoter for livestock is manufactured through the above steps, in order to actually commercialize the growth promoter for livestock, as shown in FIG. 2 , a quality analysis step and a step of packaging and storage are additionally required.

The sixth step (S6) is a step of analyzing the quality of the completed growth promoter for livestock. In the sixth step (S6), the binding of calcium and peptide in the growth promoter for livestock is checked, the properties are analyzed, or the moisture content is measured.

The binding of calcium and peptide in the growth promoter for livestock is checked by the following method.

[How to check whether calcium and peptide are bound]

1) Mix 10 g of the prepared livestock growth promoter sample with 100 g of distilled water and mix well.

2) Filter the mixture through a filter paper to separate the powder phase and the liquid phase, and additionally wash the powder phase with 100 g of distilled water.

3) Mix the filtrate phase and the washing liquid phase, and add 800 g of distilled water to it.

4) Dilute at a ratio of 1/10 and use as ICP test solution (solution containing water-soluble calcium).

5) Dry the powdered phase filtered on the filter paper at 60°C for 3 hours.

6) Mix 80g of nitric acid and 120g of distilled water. (Calcium extract)

7) Mix 100 g of calcium extract in the dried powder phase and mix well.

8) Filter the mixture through a filter paper to separate the liquid phase, and wash with 100 g of calcium extract in addition to the powder phase.

9) Mix the washing solution and the filtrate, and add 800 g of distilled water.

10) Dilute at a ratio of 1/10 and use as ICP test solution (solution containing non-aqueous calcium).

11) Analyze the two prepared ICP test solutions using ICP.

The results of the analysis through the above process are shown in <Table 1>.

<Table 1>

As a result of the analysis, if the calcium concentration of the non-aqueous calcium-containing solution and the water-soluble calcium-containing solution is 10 to 12 times, it is considered that calcium and the peptide are normally combined.

The seventh step (S7) is a step of packaging and storing the growth promoter for livestock when it is confirmed that the growth promoter for livestock is normal in the sixth step. The disclosed growth promoter for livestock should not be stored in a place exposed to high humidity or direct sunlight to prevent decay and deterioration. Also, the temperature of the storage area should not be too high. Therefore, it is preferable to store it in a dry and cool dark room.

Although the preferred embodiment of the present invention has been described with reference to the accompanying drawings, the embodiments described in the present specification and the configuration shown in the drawings are only the most preferred embodiment of the present invention and represent all of the technical spirit of the present invention. Therefore, it should be understood that there may be various equivalents and modifications that can be substituted for them at the time of filing the present application. Therefore, the embodiments described above are to be understood as illustrative and not restrictive in all respects, and the scope of the present invention is indicated by the following claims rather than the detailed description, and the meaning and scope of the claims and their All changes or modifications derived from the concept of equivalents should be construed as being included in the scope of the present invention.

S1 Step 1
S2 Step 2
S3 Step 3
S4 Step 4
S5 Step 5
S6 Step 6
S7 Step 7

Claims (6)

A first step of preparing a hydrolyzate of yeast by mixing dry inert yeast, process water, and an enzyme;
A second step of mixing the hydrolyzate of yeast with inorganic calcium, a peptide source, and process water;
a third step of drying the mixture made through the second step;
a fourth step of pulverizing the dry mixture;
a fifth step of mixing an excipient, a dispersing agent, and a stabilizer with the pulverized pulverized product to complete a growth promoter for livestock;
a sixth step, which is a quality analysis step of checking whether the calcium and peptides of the completed growth promoter for livestock are combined, their properties, and moisture content; and
A seventh step of packaging and storing the growth promoter for livestock whose quality has been analyzed;
The first step is characterized in that the yeast hydrolyzate is prepared by mixing 1 to 4 parts by weight of dry inert yeast, 3 to 24 parts by weight of process water, and 0.001 to 0.005 parts by weight of the enzyme at 40° C. for 4 hours,
The second step is characterized in that the yeast hydrolyzate is mixed with inorganic calcium, a peptide source, process water, and an excipient for 1 hour and then aged for 1 day,
The third step is characterized in that the mixture made through the second step is dried to a moisture content of 5 to 12%,
In the sixth step, the determination of whether the calcium and the peptide are bound is to check whether the ratio of the calcium concentration of the non-aqueous calcium-containing solution and the water-soluble calcium-containing solution is 10 to 12 times. Way.
delete delete delete The method according to claim 1,
The fourth step is a method for producing a growth promoter for livestock, characterized in that the dry mixture is pulverized to a particle size of 0.1 to 3 mm in diameter using any one of a hammer mill, a cutter mill and a pin mill.
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