KR102436816B1 - Cosmetic composition - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a graviola herbal extract. More particularly, the present invention relates to a cosmetic composition having an antioxidant effect, a whitening effect, an anti-wrinkle effect, and an anti-inflammatory effect.
The cosmetic composition having antioxidant effect, whitening effect, wrinkle improvement effect and anti-inflammatory effect according to the present invention is a human-friendly natural cosmetic, and contains graviola water with antibacterial activity, so it is harmless to human skin and easily accessible even to people with sensitive skin. Can be used. In addition, it effectively acts to promote hair growth, stimulates the scalp hair root, helps prevent hair loss, and has the advantage of effectively growing hair.

Description

Cosmetic composition comprising graviola herbal extract {Cosmetic composition}

The present invention relates to a cosmetic composition comprising a graviola herbal extract, and to a cosmetic composition having an antioxidant effect, a whitening effect, an anti-wrinkle effect, and an anti-inflammatory effect.

About 80 to 100 hairs fall out a day normally, and new hairs are formed in the places where they fall out. Hair loss refers to a condition in which the number of hairs falling out is greater than the number of newly formed hairs. The causes of hair loss vary depending on the individual, but genetic factors, scalp and health care conditions, and stress are the main causes. When blood circulation is good, nutrients and oxygen are smoothly supplied to the hair roots, and hair grows healthy. However, if blood circulation is not smooth due to high fat diet or smoking, hair loss is promoted. In modern society, the number of people suffering from hair loss is increasing and the age group is getting lower.

Among the conventional drugs for promoting hair growth for the treatment of alopecia, the products that have been approved for the efficacy as a hair loss treatment by the US Food and Drug Administration (FDA) so far include oral finasteride (Finasteride, Merk, USA) and topical minoxidil (Minoxidil, Upjohn, United States), two are known. Minoxidil has a capillary dilatation function, so it has an effect of preventing hair loss by applying it to the scalp, but it has to be applied to the scalp continuously, and the effect is shown only when the drug stays on the scalp for a certain period of time, and side effects such as itching and irritation are appearing. Finasteride has a function of inhibiting the production of DHT (5α-dihydrotestosterone), a male hormone that causes hair loss, and has an effect of inhibiting hair loss when taken for a long time, but there is a problem that it returns to its original state when taking it is stopped. In addition, as side effects, there are decreased sexual function such as decreased libido and erectile dysfunction, and it is known that women should not take it because there is a risk of giving birth to a deformed child. That is, the conventionally used anti-hair loss agent uses a chemical substance, and because it stimulates the hair root by expanding capillaries by temporary blood flow circulation, the effect is not only temporary, but the effect is limited only to hair loss due to excessively secreted sebum. there is also

According to a recent study report, it is known that when chemically induced oxidative stress is applied to the scalp, the hair cycle is significantly shortened and hair loss induced by the oxidation of sebum occurs. In addition, it is known that various antioxidants are effective in preventing hair loss and promoting hair growth.

Attempts to prevent hair loss and promote hair growth by applying or taking herbal extracts to the scalp are also well known. Republic of Korea Patent Publication No. 10-780180 (when using sukjihwang, cornflower oil, texas, sanyak, bokryeong and mulberry), Korean Patent Publication No. 10-1103651 (6 kinds of arborvitae, ginseng, dansam, peppermint, hanryeoncho, and sagebrush) Many studies have been conducted to solve the above problems, such as when extracts of herbal herbal preparations are used) and Republic of Korea Patent Publication No. 10-1155502 (in the case of using extracts of sangbaekpi, licorice, uiin, brown algae, ginseng, ginseng and ginkgo leaves) Although progress has been made, various therapeutic agents for preventing hair loss and improving the scalp have been proposed, but to date, they are not suitable for fundamentally solving the above problems.

Accordingly, the present inventors have tried to develop a novel anti-hair loss composition that overcomes the limitations of the prior art. As a result, the antibacterial activity of graviola extract containing antioxidants phytochemicals and acetogenin, skin The present invention was completed by confirming the effect of improving the proliferation of cells and confirming the excellent effect of preventing hair loss and improving the scalp of a hair shampoo in which graviola extract, herbal extract and copper peptide were combined.

Republic of Korea Patent Publication No. 10-780180 Republic of Korea Patent Publication No. 10-1103651 Republic of Korea Patent Publication No. 10-1155502

Blois MS. 1958, Nature, 26, 1199-1120. Roberta R et al., 1999, Free radical biology & Medicine, 26(9/10), 1231-1237. Stirpe F et al., 1969, J. Biol. Chem., 244(14), 3855-3863. Marklund S et al., 1974, Eur J Biochem., 47(3), 469-474. Carmichael J et al., 1987, Cancer Res., 47(4), 936-942. Yagi A et al., 1986, 3981, 517-519. Hosoi J et al., 1985, Cancer Res., 45(4), 1474-1478.

As such, an object of the present invention is to provide a hair cosmetic composition for preventing hair loss and improving the scalp, which is based on the graviola extract and further comprising an herbal extract and copper tripeptide, and a method for preparing the same.

In addition, it has an antioxidant effect, a whitening effect, an anti-wrinkle effect, and an anti-inflammatory effect.

In order to achieve the object of the present invention, in one embodiment, the present invention provides a method for preparing a cosmetic composition having an antioxidant effect, a whitening effect, an anti-wrinkle effect and an anti-inflammatory effect, comprising the following steps: 1) purified water, carbo Filtration after dissolving the mer and disodium EDTA by heating to 90° C.; 2) cyclonic acid, pine needle fruit extract, glycerin, dimethicone, sodium lauryl sulfate and cocamidopropyl betaine were dissolved by heating to 70° C., followed by mixing and stirring with the filtrate of step 1); 3) Cocamide DEA, iodopropynyl butyl carbamate and glycol distearate were dissolved by heating to 70°C, filtered, mixed with the product of step 2), and cooled to 60°C; 4) Cetrimonium chloride, fragrance, triethanolamine, sodium chloride, hydrolyzed keratin, sewage extract, red ginseng extract, white paper extract, orchid extract, Angelica asiatica extract, pomegranate extract, rhubarb extract, copper tripeptide, graviola ethanol extract, A step of mixing and stirring sodium laureth ether sulfate and graviola hot water extract with the product of step 3) is provided, cooling to 60° C. and filtering. In the above embodiment, 13 to 17 parts by weight of purified water, 0.1 to 0.3 parts by weight of carbonner, 0.05 to 0.1 parts by weight of disodium EDTA, 0.1 to 0.3 parts by weight of citric acid, 0.1 to 0.3 parts by weight of pine needle fruit extract, Glycerin 1.5 to 2.0 parts by weight, dimethicone 1.5 to 2.5 parts by weight, sodium lauryl sulfate 8 to 12 parts by weight, cocamidopropyl betaine 6 to 8 parts by weight, cocamide DA 6 to 8 parts by weight, iodopropy 0.03-0.05 parts by weight of nylbutyl carbamate, 0.3-0.5 parts by weight of glycol distearate, 1.8-2.2 parts by weight of cetrimonium chloride, 0.3-0.7 parts by weight of fragrance, 0.3-0.7 parts by weight of triethanolamine, 0.3 parts by weight of sodium chloride to 0.7 parts by weight, hydrolyzed keratin 0.8 to 1.2 parts by weight, sewage extract 0.13 to 0.15 parts by weight, red ginseng extract 0.13 to 0.15 parts by weight, white paper extract 0.13 to 0.15 parts by weight, chrysanthemum extract 0.13 to 0.15 parts by weight, Angelica Root extract 0.13 to 0.15 parts by weight, pomegranate extract 0.13 to 0.15 parts by weight, rhubarb extract 0.13 to 0.15 parts by weight, copper tripeptide 0.15 to 0.25 parts by weight, graviola ethanol extract 0.4 to 0.6 parts by weight, sodium laureth ether sulfate 23 to 27 weight It provides a method, characterized in that it is mixed in parts and 23 to 27 parts by weight of the graviola hot water extract, more specifically, 15 parts by weight of purified water, 0.2 parts by weight of carboner, 0.07 parts by weight of disodium EDTA, cyclonic acid 0.2 parts by weight, pine needle fruit extract 0.2 parts by weight, glycerin 1.7 parts by weight, dimethicone 2 parts by weight, sodium lauryl sulfate 10 parts by weight, cocamidopropyl betaine 7 parts by weight, cocamide DA 7 parts by weight, IO 0.04 parts by weight of dopropynyl butyl carbamate, 0.4 parts by weight of glycol distearate, 2 parts by weight of cetrimonium chloride, 0.5 parts by weight of fragrance, 0.5 parts by weight of triethanolamine, 0.5 parts by weight of sodium chloride parts by weight, hydrolyzed keratin 1 part by weight, sewage extract 0.143 parts by weight, red ginseng extract 0.143 parts by weight, white paper extract 0.143 parts by weight, orchid extract 0.143 parts by weight, Angelica root extract 0.143 parts by weight, pomegranate extract 0.143 parts by weight, rhubarb extract 0.143 parts by weight, 0.2 parts by weight of copper tripeptide, 0.5 parts by weight of graviola ethanol extract, 25 parts by weight of sodium laureth ether sulfate and 25 parts by weight of graviola hot water extract.

In another embodiment of the present invention, there is provided a cosmetic composition having an antioxidant effect, a whitening effect, an anti-wrinkle effect, and an anti-inflammatory effect, comprising the product prepared in the above embodiment as an active ingredient.

The hair cosmetic composition for preventing hair loss and improving scalp according to the present invention is a human-friendly natural cosmetic, containing graviola extract with low cytotoxicity and antibacterial activity, so it is harmless to human skin and can be easily used even by people with sensitive skin. In addition, it effectively acts to promote hair growth, stimulates the scalp hair root, helps prevent hair loss, and has the advantage of effectively growing hair.

In addition, it has an antioxidant effect, a whitening effect, an anti-wrinkle effect, and an anti-inflammatory effect.

1 to 4 show the results of measuring the antioxidant effect of the graviola extract.
5 shows the results of measuring the cytotoxicity of the graviola extract.
6 shows the results of measuring the tyrosinase inhibitory ability of the graviola extract.
7 shows the effect of the graviola extract on procollagen synthesis.
8 shows the results of measuring the antimicrobial activity of the graviola extract.
9 shows the effect of the graviola extract on the synthesis of antibacterial peptides.
10 shows the results of measuring the wrinkle improvement effect of the graviola extract by the elastase inhibitory ability.
11 and 12 show the results of measuring the anti-inflammatory effect of the graviola extract.

Hereinafter, the components and technical features of the present invention will be described in more detail through the following examples. However, the following examples are merely illustrative of the content of the present invention, and the scope of the present invention is not limited by the examples.

Example

Preparation of extracts

1.1 Graviola Ethanol Extract

Graviola leaves and ethanol (70%) were mixed in a weight ratio of 1:100 to prepare an extract in a 24H rolling extractor. The prepared extract was dried and made into a powder.

1.2 Graviola Hot Water Extract

An extract was prepared by boiling graviola leaves and cosmetic purified water at a weight ratio of 1:200 to 100°C.

1.3 Danose Water Extract

Rhubarb, chrysanthemum, rhododendron, white pomegranate, ginseng and angelica were mixed with butylene glycol and glycerin to prepare an aqueous solution, followed by hot water bathing at 70°C to 80°C. After filtering with a 1 μm filter, it was allowed to cool and left at room temperature. After performing secondary filtration with 0.8 μm and 0.45 μm filters, preservatives were treated, and then tertiary filtration was performed with 0.2 μm filters.

efficacy test

2.1 Antioxidant effect

1) DPPH radical scavenging ability

DPPH electron donating ability was measured according to the method of Blois (Non-Patent Document 1). To 2 mL of each sample solution containing the graviola extract, 1 mL of 0.2 mM 1,1-diphenyl-2-picrylhydrazyl (DPPH) was added and stirred. After standing for 30 minutes, absorbance was measured at 517 nm. The electron donating ability was expressed as the absorbance decrease rate of the addition and non-addition groups of the sample solution. As a result of measuring the DPPH radical scavenging ability of the graviola hot water extract and the ethanol extract, 1,000 ug/ml of the graviola ethanol extract confirmed a superior efficacy than the positive control group vitamin-C (Vit-C) (Fig. 1, hot water extract 79%, Ethanol extract 91%, vitamin C 83%).

2) ABTS cation radical scavenging ability

The antioxidative power measurement using the ABTS (2,2'-azino-bis(3-ethylbenzthiazoline)-6-sulphonic acid) cation radical was measured by the ABTS cation decolorization test (Non-Patent Document 2). 7mM 2,2-azino-bis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) and 2.4mM potassium persulfate were mixed to a final concentration and left for 24 hours in a dark place at room temperature to add ABTS ⁺ After forming a phosphate buffered saline (PBS) diluted with ABTS ⁺ 100 uL was added, left for 1 minute, and then absorbance was measured at 732 nm. As a result of measuring the ABTS ⁺ radical scavenging ability of the graviola hot water extract and the graviola ethanol extract, 500 μg/ml of the graviola ethanol extract confirmed the superior efficacy than the positive control group vitamin-C (FIG. 2).

3) Superoxide anion radical scavenging ability

Superoxide anion radical scavenging ability was measured by NBT (nitrobule tetrazolium) reduction method (Non-Patent Document 3). To 0.1 mL of each sample solution and 0.4 mL of 0.1M potassium phosphate buffer (pH 7.5), add 1 mL of a substrate solution in which 0.4 mM Xanthine and NBT (0,24 mM) were dissolved, and xanthine oxidase (0,2 U/ ML) 1 ML was added, followed by reaction at 37° C. for 20 minutes, and then 1 mL of 1 N hydrochloric acid was added to terminate the reaction. The amount of superoxide anion radicals generated in the reaction solution was measured by absorbance at 560 nm. As a result of measuring the superoxide anion radical scavenging activity of the graviola hot water extract and the graviola ethanol extract, 1,000 μg/ml of the graviola hot water extract showed a superior efficacy than the positive control group Vit-C (FIG. 3).

4) Superoxide dismutase (SOD)-like activity

SOD-like activity was measured according to Marklund's method (Non-Patent Document 4). In 0.2 mL of each sample solution, 2.6 mL of Tris-HCl buffer (50 mM Tris+10 mM EDTA, pH 8.5) and 7.2 mM pyrogallol were measured at 420 nm. The SOD-like activity was expressed as the absorbance reduction rate of the experimental group and the control group of the sample solution. At 1,000 ug/ml of graviola hot water extract and graviola ethanol extract, efficacy effects of 10.27% and 22.2% were confirmed, respectively (FIG. 4). The activity of antioxidant enzymes that remove free radicals and toxins in the human body was measured. The antioxidant power was excellent, but the activity of SOD (antioxidant enzyme) was significantly lower than that of vitamin C.

2.2 Cytotoxicity

In the present invention, each cell was cultured using 10% fetal bovine serum (FBS) and Dulbeco's modified eagle's medium (DMEM) medium supplemented with , and subcultured at 37° C., adapted to a 5% CO2 incubator.

Cell viability was measured according to Camichael's method (Non-Patent Document 5). The MTT screening method to check the cell viability uses a 96-well plate and can easily read many samples using an ELISA reader (multiwell microplate reader), which is one of the methods widely used as a cytotoxicity and cell proliferation screening method. to be. Each cell melanoma (B16F10), fibroblast (CCRF), macrophage (Raw264.7) cells were dispensed in a 96-well plate at a volume of 0.6~8x10 ³ cells/well, and 0.18 mL of each sample was prepared by concentration, added 0.02 mL, and then added at 37°C. , 5% CO ₂ Incubated for 24 hours in an incubator. The control group was cultured under the same conditions by adding the same amount of sterile water as the sample. Here, 0.02 mL of MTT solution prepared at a concentration of 5 mg/mL was added, incubated for 4 hours, the culture medium was removed, and 0.15 mL of DMSO: Ethanol (1: 1) was added to each well, reacted at room temperature for 30 minutes, and then ELISA reader Absorbance was measured at 550 nm. Cell viability was measured as a decrease in absorbance of the group with and without the addition of the sample solution. As a result of the MTT assay (B16F10 melanoma) test, the graviola hot water extract showed a cell viability of 80% or more at a concentration of 10 ug/ml, and the graviola ethanol extract confirmed a cell viability of 80% or more at a concentration of 1 ug/ml (FIG. 5) .

2.3 Whitening effect

1) Inhibitory ability of mushroom-derived tyrosinase

Tyrosinase inhibitory ability was measured according to the method of Yagi et al. (Non-Patent Document 6). The reaction zone is a mixture of 0.2 mL of a substrate solution in which 10 mM L-DOPA is dissolved in 0.5 mL of 0.175 M sodium phosphate buffer (pH 6.8) and 0.1 mL of the sample solution by adding 0.2 mL of mushroom tyrosinase (110 U/mL) to 37? At 475 nm, the DOPA chrome produced in the reaction solution was measured at 475 nm by reacting for 2 minutes. The tyrosinase inhibitory ability was expressed as the absorbance reduction rate of the group with and without the addition of the sample solution (FIG. 6).

2) Inhibition of melanin biosynthesis in B16F10

Melanin biosynthesis inhibitory ability was measured according to the method of Hosoi (Non-Patent Document 7). The melanoma cells cultured in DMEM medium were aliquoted to 2 x 10 ⁶ cells/dish in a 100 mm culture dish. After culturing for 24 hours, samples were prepared by concentration, 2 mL was added, and washed with PBS (pH 7.4) after 48 hours. . Then, after detaching the cells with 0.25 M trypsin-EDTA solution, the harvested cells were treated with 1 mL of 5% TCA per 1 x 10 ⁶ cells, centrifuged twice at 2,500 rpm, and the separated melanin was washed with PBS. Then, 1 mL of ether: ethanol (1:3) was added, centrifuged twice, and washed and dried with 1 mL of ether. 1 mL of 1 N NaOH was added to the dried melanin, reacted at 80° C. for 1 hour, and absorbance was measured at 405 nm spectrophotometer. Inhibition of melanin biosynthesis was expressed as a decrease in absorbance in the group with and without the sample solution.

3) Pro-collagen synthesis rate

The effect of the graviola extract on procollagen synthesis was measured (FIG. 7).

2.4 Antibacterial

The liquid medium for pre-culture and main culture was nutrient broth (NB) as a liquid medium for Staphylococcus epidermidis (S. epidermidis) and Escherichia coli (E. coli) and tryptic as a liquid medium for Staphylococcus aureus (S. aureus). Soy broth (TSB) was used, and for Propionibacterium acnes (P. acnes), Gifu anaerobic medium (GAM) medium was used. Nutrient agar (NA) was used for the solid medium of S. epidermidis and E. coli, and tryptic soy agar (TSA) was used as the liquid medium for S. aureus, and P.acnes was cultured using Gifu anaerobic medium (GAM). ) was used in this experiment by adding agar to the medium. P.acnes bacteria were cultured at 37°C in a 5% CO2 incubator, and all other strains were cultured at 37°C in a BOD incubator.

The antibacterial activity of graviola hot water extract was tested. As a control, normal purified water was used. Staphylococcus aureus and E. coli were cultured in a medium containing graviola water and general purified water for 4 weeks to confirm the antibacterial activity. Antibacterial activity was measured by the paper disc method. 1 Platinum lice of each strain cultured as a single colony on a plate medium was taken and cultured in 10 mL of liquid medium for 18 to 24 hours for activation. The number of bacteria per plate medium was inoculated to about 1×107 cells and spread evenly with a sterile cotton swab. After placing a sterilized filter paper disc (8 mm, Whatman, Japan) on a solid plate medium, absorb the samples by concentration so as to become 50 μL/disc, incubate at 37°C for 18 to 24 hours, and clear zone (mm) around the disc. was measured (Fig. 8).

2.5 Expression of Antimicrobial Peptides

After the graviola extract was treated on human keratinocytes, the expression of Cathelicidin antimicrobial peptide (CAMP) and LL-37, which are antibacterial peptides expressed in the skin cells themselves, was confirmed. In order to check the activity of MITF, TRP-1, TRP-2, MMP-1, iNOS, and COX-2, cell lines (B16F10, CCRF, Raw246.7) were seeded in a tissue culture dish and then cultured for 24 hours. stabilized. After removing the medium, the sample was incubated for 24 to 48 hours with a medium treated for each concentration, the medium was removed again, and washed twice with PBS. Complete mini 1 tab was added to 10 ml of RIPA buffer, dissolved in 100 uL, and centrifuged at 4°C and 12,000 rpm for 20 minutes. Proteins obtained by centrifugation were quantified by Bradford assay using bovine serum albumin (BSA) as a standard, and electrophoresed at 120 V for 1 hour using 10% SDS-polyacrylamide gel. After loading, polyvinylidenedifluoriden (PVDF) was used for transfer at 30-40 V for 2 hours or more. After transfer, the band was checked after immersion in ponceau S, washed twice or more with TBST, and incubated overnight with 5% skin milk to remove the background. After washing 3 times, the primary antibody (1: 1000) was attached for 1 hour, the secondary antibody (1: 1000) was attached and transferred to the film using an ECL kit (Amersham Pharmacia, England) for measurement. Band density was confirmed by Gel doc (Bio-rad, America) (FIG. 9).

2.6 Wrinkle improvement effect

The wrinkle improvement effect of the graviola extract was measured by measuring the elastase inhibitory ability. Elastase inhibitory ability was measured according to the method of Cannell et al. (Non-Patent Document 7). Using N-succinyl-(L-Ala)3-p-nitroanilide as a substrate, the amount of p-nitroanilide produced from the substrate was measured at 405 nm at 37°C for 20 minutes. That is, prepare each test solution to a certain concentration, take 0.5 mL of each test tube, add 0.5 mL of porcine pancreas elastase (2.5 U/mL) solution dissolved in 50 mM tris-HCl buffer (pH 8.6), and then add 50 mM as a substrate N-succinyl-(L-Ala)3-p-nitroanilide (0.5 mg/mL) dissolved in tris-HCl buffer (pH 8.6) was added, followed by reaction for 20 minutes. The elastase inhibitory ability was expressed as the absorbance reduction rate of the group with and without the addition of the sample solution (FIG. 10).

2.7 Anti-inflammatory effect

Nitric oxide (NO) was measured as nitrite and nitrate in the amount of NO in the supernatant of the cell (Non-Patent Document 8). Griess reagent (Sigma, USA), which is a safe form after reduction with nitrate for nitrite, was used. When confluence was 80%, 2 × 106 cells in a 6 well plate were washed twice with PBS and then serum-free medium was used. After incubation for more than 12 hours, 10 μg/mL of lipopolysacchride (LPS) was added to all wells except the control group and stimulated. After 2 hours, samples were treated by concentration and tested. The amount of NO production was measured by absorbance at 540 nm after collecting the supernatant after 24 hours and reacting with griess regent for 10 minutes (FIG. 11). In addition, the iNOS expression inhibitory effect of the graviola extract was measured (FIG. 12).

Preparation of hair cosmetic composition

Based on 100 parts by weight of the hair cosmetic composition according to the present invention, 1) 0.2 parts by weight of Carbomer and 0.07 parts by weight of Disodium EDITA were placed in 15 parts by weight of purified water and dissolved by heating to 90°C. The lysate was filtered; 2) Mix 0.2 parts by weight of citric acid, 0.2 parts by weight of pine needle fruit extract, 1.7 parts by weight of glycerin, 2 parts by weight of dimethicone, 10 parts by weight of sodium lauryl sulfate and 7 parts by weight of cocamidopropyl betaine until 70° C. After dissolution by heating, the mixture was stirred with the filtrate of step 1); 3) 7 parts by weight of cocamide DA, 0.04 parts by weight of iodopropynyl butyl carbamate and 0.4 parts by weight of glycol distearate were dissolved by heating to 70° C., filtered, and mixed with the product of step 2) and stirred, cooled to 60° C.; 4) 2 parts by weight of cetrimonium chloride, 0.5 parts by weight of fragrance, 0.5 parts by weight of triethanolamine, 0.5 parts by weight of sodium chloride, 1 part by weight of hydrolyzed keratin, 0.143 parts by weight of sewage extract, 0.143 parts by weight of red ginseng extract, white paper extract 0.143 parts by weight, orchid extract 0.143 parts by weight, Angelica asiatica extract 0.143 parts by weight, pomegranate extract 0.143 parts by weight, rhubarb extract 0.143 parts by weight, copper tripeptide 0.2 parts by weight, graviola extract 0.5 parts by weight, sodium laureth ether sulfate 25 parts by weight And 25 parts by weight of purified water substitute (graviola water) was mixed and stirred with the mixture cooled to 60°C of step 3), cooled to 35°C, and filtered to prepare a hair cosmetic composition.

Claims (4)

A method for preparing a cosmetic composition having an antioxidant effect, a whitening effect, an anti-wrinkle effect, and an anti-inflammatory effect, comprising the following steps:
1) dissolving purified water, carbomer and disodium EDTA by heating to 90° C., followed by filtering;
2) cyclonic acid, pine needle fruit extract, glycerin, dimethicone, sodium lauryl sulfate and cocamidopropyl betaine were dissolved by heating to 70° C., followed by mixing and stirring with the filtrate of step 1);
3) Cocamide DEA, iodopropynyl butyl carbamate and glycol distearate were dissolved by heating to 70°C, filtered, mixed with the product of step 2), and cooled to 60°C;
4) Cetrimonium chloride, fragrance, triethanolamine, sodium chloride, hydrolyzed keratin, sewage extract, red ginseng extract, white paper extract, orchid extract, Angelica asiatica extract, pomegranate extract, rhubarb extract, copper tripeptide, graviola ethanol extract, A step of mixing and stirring sodium laureth ether sulfate and graviola hot water extract with the product of step 3), cooling to 60° C., and filtering.
The method of claim 1,
13 to 17 parts by weight of purified water, 0.1 to 0.3 parts by weight of carboner, 0.05 to 0.1 parts by weight of disodium EDTA, 0.1 to 0.3 parts by weight of cyclonic acid, 0.1 to 0.3 parts by weight of pine needle extract, 1.5 to 2.0 parts by weight of glycerin parts, dimethicone 1.5 to 2.5 parts by weight, sodium lauryl sulfate 8 to 12 parts by weight, cocamidopropyl betaine 6 to 8 parts by weight, cocamide DA 6 to 8 parts by weight, iodopropynyl butyl carbamate 0.03 to 0.05 parts by weight, glycol distearate 0.3 to 0.5 parts by weight, cetrimonium chloride 1.8 to 2.2 parts by weight, fragrance 0.3 to 0.7 parts by weight, triethanolamine 0.3 to 0.7 parts by weight, sodium chloride 0.3 to 0.7 parts by weight, 0.8 to 1.2 parts by weight of hydrolyzed keratin, 0.13 to 0.15 parts by weight of sewage extract, 0.13 to 0.15 parts by weight of red ginseng extract, 0.13 to 0.15 parts by weight of white paper extract, 0.13 to 0.15 parts by weight of orchid extract, 0.13 to 0.15 parts by weight of angelicae extract , pomegranate extract 0.13 to 0.15 parts by weight, rhubarb extract 0.13 to 0.15 parts by weight, copper tripeptide 0.15 to 0.25 parts by weight, graviola ethanol extract 0.4 to 0.6 parts by weight, sodium laureth ether sulfate 23 to 27 parts by weight, and graviola hot water Method, characterized in that the extract is mixed with 23 to 27 parts by weight.
The method of claim 1,
Purified water 15 parts by weight, Carboner 0.2 parts by weight, Disodium EDITA 0.07 parts by weight, Cyclic acid 0.2 parts by weight, Pine pine fruit extract 0.2 parts by weight, Glycerin 1.7 parts by weight, Dimethicone 2 parts by weight, Sodium lauryl sulfate 10 parts by weight, cocamidopropyl betaine 7 parts by weight, cocamide DA 7 parts by weight, iodopropynyl butyl carbamate 0.04 parts by weight, glycol distearate 0.4 parts by weight, cetrimonium chloride 2 parts by weight , fragrance 0.5 parts by weight, triethanolamine 0.5 parts by weight, sodium chloride 0.5 parts by weight, hydrolyzed keratin 1 part by weight, sewage extract 0.143 parts by weight, red ginseng extract 0.143 parts by weight, white paper extract 0.143 parts by weight, orchid extract 0.143 parts by weight , angelica extract 0.143 parts by weight, pomegranate extract 0.143 parts by weight, rhubarb extract 0.143 parts by weight, copper tripeptide 0.2 parts by weight, graviola ethanol extract 0.5 parts by weight, sodium laureth ether sulfate 25 parts by weight and graviola hot water extract 25 parts by weight A method comprising mixing.
A cosmetic composition having an antioxidant effect, a whitening effect, an anti-wrinkle effect, and an anti-inflammatory effect prepared by the method of any one of claims 1 to 3.

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