KR102152407B1 - Cosmetic composition containing phycocyanobilin and its derivative derived from spirulina - Google Patents

Abstract

The cosmetic composition according to an aspect of the present invention comprises phycocyanobilin derived from spirulina and derivatives thereof as an active ingredient. Accordingly, the present invention exhibits remarkable effects in the treatment of skin inflammatory diseases, anti-ultraviolet effect, skin wrinkle improvement, and skin whitening improvement.

Description

Cosmetic composition containing phycocyanoblin derived from spirulina and its derivatives as active ingredients {COSMETIC COMPOSITION CONTAINING PHYCOCYANOBILIN AND ITS DERIVATIVE DERIVED FROM SPIRULINA}

The present invention relates to a cosmetic composition, and more particularly, to a cosmetic composition comprising a phycocyanoblin derived from spirulina and a derivative thereof as an active ingredient.

Spirulina (Spirulina) is the oldest algae (藻類 · algae) on Earth, a multicellular organism with thin cell walls. Spirulina was used in ancient Africa and Mexico, but only recently was introduced to the modern industrial society.

The color of Spirulina is emitted by the phycocyanin (blue) of chlorophyll (green) in the cell, and the blue-green algae is also called blue-green algae (藍藻). The scientific name of Spirulina was given in 1827 by Turpin, a German ornithologist.

Spirulina means spiral (D) in Latin. It grows naturally in salt lakes in tropical regions and is not widely distributed worldwide, but the optimum water temperature is 32~42℃, and it breeds in a strong alkaline environment.

Spirulina is a high-protein food that contains more protein (69.5%) than chlorella (50%), which is famous for being high in protein, and contains all essential amino acids in a good balance. It is easy to see how much protein Spirulina has compared to 39% of soybeans (soybeans), 20% of beef, and 12% of eggs. In addition, Spirulina is multi-celled and has a high digestibility and absorption rate (95.1%) because it does not have a cell wall, but chlorella is a single cell and has a low digestibility (73.6%) due to its thick cell membrane.

Spirulina's pigment components contain yellow carotenoids, green chlorophyll, and blue phycocyanin. Pycocyanin is a bile pigment contained in Spirulina, which is not present in chlorella, and like animal bile pigments, it helps digest fat and exhibits anti-inflammatory and antioxidant properties. It also contains a large amount of antioxidants such as tocopherol and beta-carotene.

Accordingly, Prior Art Document 1 discloses a method of manufacturing a cosmetic product having a moisturizing and anti-inflammatory effect using Spirulina. However, the prior art document 1 is merely a situation where spirulina is pulverized and filtered.

In addition, phycocyanin contained in Spirulina is excellent in physiological activity, but is a very unstable substance. That is, there is a disadvantage that the structural destruction of phycocyanin occurs due to a strong interaction that occurs according to factors such as light and temperature after extraction, leading to a decrease in the concentration of phycocyanin contained in the extract. Therefore, it is necessary to derive a substance with a more stable structure than phycocyanin.

[Prior technical literature]

Korean Patent Registration No. 10-1127392 (2012. 03. 23)

The present invention is to solve the problems of the prior art, and an object of the present invention is to provide a cosmetic composition by preparing phycocyanobilin from spirulina.

In addition, the present invention has a more stable structure in order to overcome the disadvantages leading to a decrease in the concentration of phycocyanin contained in the extract due to the structural destruction of phycocyanin due to the strong interaction that occurs depending on factors such as light and temperature. It is to provide a cosmetic composition converted to a substance, phycocyanobilin.

The cosmetic composition according to an aspect of the present invention comprises phycocyanobilin derived from spirulina and derivatives thereof as an active ingredient.

At this time, the spirulina may be selected from spirulina planters (spirulina platensis) or spirulina maxima (spirulina maxima).

In addition, the phycocyanobilin may be prepared by centrifuging an aqueous spirulina solution, adding TCA to the separated supernatant, stirring, and then concentrating and co-drying the reaction product to which MeOH was added to the precipitate.

In addition, the phycocyanobilin and derivatives thereof may be (3E)-PCB or (3Z)-PCB.

In addition, the phycocyanobilin and derivatives thereof are contained in an amount of 0.0001 to 20% by weight based on the total weight of the cosmetic composition, and may be used to improve skin inflammatory diseases.

In addition, preferably the phycocyanobilin and derivatives thereof may be contained in an amount of 0.4 to 17% by weight based on the total weight of the cosmetic composition,

More preferably, the phycocyanobilin and derivatives thereof may be contained in an amount of 4 to 11% by weight based on the total weight of the cosmetic composition.

In addition, the skin inflammatory disease at this time may be acne dermatitis or atopic dermatitis.

In addition, the cosmetic composition has a dermatitis improvement effect through antibacterial activity against Propionebacterium acne (Propionebacterium acne), which is a trigger of acne dermatitis, and Staphylococcus aureus, which is a pyogenic bacteria.

Meanwhile, the phycocyabiline and derivatives thereof are contained in an amount of 0.0001 to 20% by weight based on the total weight of the cosmetic, and may be used to improve skin wrinkles and skin whitening. In this case, the phycocyaviline and derivatives thereof may be contained in an amount of 2 to 10% by weight based on the total weight of the cosmetic.

In addition, the phycocyaviline and derivatives thereof are contained in an amount of 1 to 20% by weight based on the total weight of the cosmetic, and may be used for anti-ultraviolet purposes.

In addition, the cosmetic composition is skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, soap, shampoo , Cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, lipstick, makeup base, foundation, press powder, and loose powder.

The present invention prepares a composition comprising a stable substance, phycocyanobilin and derivatives thereof, and exhibits remarkable effects in treating skin inflammatory diseases, anti-ultraviolet rays, skin wrinkles, and skin whitening.

In addition, the present invention provides a further effect of obtaining a composition that is safe against heat and light and has excellent cosmetic activity through the first and second processing processes of spirulina simple extracts.

1 is a graph measuring the fibroblast proliferation effect of a composition containing phycocyanobrin (PCB) from Spirulina.
Figure 2 is a graph measuring the elastase activity inhibitory effect of a composition containing phycocyanobrin (PCB) from Spirulina.
3 is a graph showing the results of measuring the melanin production inhibitory effect of a composition containing phycocyanobrin (PCB) from spirulina.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art can easily implement them. The present invention will be described in detail by exemplifying specific embodiments in the drawings, since various modifications may be made and various embodiments may be provided. However, this is not intended to limit the present invention to a specific embodiment, it is to be understood to include all changes, equivalents, and substitutes included in the spirit and scope of the present invention.

Terms including ordinal numbers, such as first and second, may be used to describe various elements, but the elements are not limited by the terms. These terms are used only for the purpose of distinguishing one component from another component.

For example, without departing from the scope of the present invention, a first element may be referred to as a second element, and similarly, a second element may be referred to as a first element. The term and/or includes a combination of a plurality of related listed items or any of a plurality of related listed items.

Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention belongs. Terms such as those defined in a commonly used dictionary should be interpreted as having a meaning consistent with the meaning in the context of the related technology, and should not be interpreted as an ideal or excessively formal meaning unless explicitly defined in this application. Does not.

Hereinafter, an embodiment of the present invention will be described.

<Example 1>

From Spirulina Phycocyanobilin Method for preparing the contained composition

To prepare a composition containing phycocyanobilin (PCB) from the spirulina used in the experiment, 50g of spirulina powder was added to 1.5L of water and stirred for 10 minutes. Then, put into six 250mL centrifuge bottles and centrifuged for 1 hour at 8000 RPM and 4°C. After centrifugation, the obtained supernatant is transferred to a 2 L flask, and then 15 g of TCA is added.

Stir for 1 hour in dark conditions (covered with aluminum foil), and then centrifuge for 10 minutes at 8000 RPM and 4°C to collect the precipitate. Thereafter, this precipitate was placed in a 500 mL Erlenmeyer flask, 25 mL of pure methanol (MeOH) was added thereto, stirred slowly, and then pure methanol was continuously added until the final total volume reached 250 mL.

After centrifugation at 8000 RPM and 4℃ for 10 minutes, discard the supernatant. Repeat the above steps four times or until the MeOH wash is colorless, and then store at -20°C while wrapped in foil. Thereafter, the pellet obtained from the extraction flask and 1 L of MeOH were added, followed by reaction at 60° C. for 12 hours. Thereafter, the obtained final reactant was concentrated and freeze-dried to obtain a powder containing PCB and used in the experiment.

<Experimental Example 1>

From Spirulina Phycocyanobilin Measurement of PCB content of the contained composition

The PCB content of the powder obtained in the above process was measured. For the analysis of the concentration of the PCB from the PCB powder obtained in Example 1, 10 mL of distilled water was added to 10 mg of powder and mixed, and the test solution was compared with distilled water to measure absorbance at a liquid layer of 1 cm and a wavelength of 680 nm.

-Analysis and calculation

PCB content (mM) = E680nm/37.9 × dilution factor

PCB content analysis result PCB content (mM) Sample 0.004

As shown in Table 1, in the method for preparing phycocyanobilin according to the present example, it was confirmed that the phycocyanobilin has a content of about 0.004 mM.

<Experimental Example 2>

From Spirulina Phycocyanobilin Measurement of the effect of the contained composition to inhibit acne formation

In order to confirm the effect of inhibiting acne generation of the composition of the present invention prepared in Example 1, propionebacterium acne, which is an acne-causing bacteria, and Staphylococcus aureus, which is a pyogenic bacteria, Antibacterial activity was measured, and the presence or absence of acne improvement/sebum secretion/irritation was measured through clinical trials.

Acne and purulent bacteria were cultured on a brain heart infusion (BHI) medium for 18 hours, and then the acne-causing bacteria, Propionebacterium, were added to the cultured medium to which a sample diluted step by step in a 96 well plate was added. Acne (Propionebacterium acne) and Pneumococcal Staphylococcus aureus were separately inoculated at a concentration of 5% (10ul). After inoculation, culture was performed at 37° C. for 24 hours, and the number of cells was measured through microscopic observation of the strain to measure antibacterial activity.

Measurement of antibacterial activity of Propionebacterium acne and Staphylococcus aureus according to the concentration of the composition containing Phycocyanobilin (PCB) from Spirulina Sample concentration (%) Propionebacterium acne (ppm) Propionebacterium acne (ppm) 0.1 <17 <13 0.5 <8 <11 One <6 <8 2 <5 <2 5 <2 <1 10 <1 <1 15 <0.7 <0.7 20 <0.6 <0.6

As a result, as shown in [Table 2], propionebacterium acne, an acne-causing bacteria, and Staphylococcus au, a pyogenic bacteria, depending on the concentration of the composition containing phycocyanobilin (PCB) from Spirulina. The concentration of reus (Staphylococcus aureus) was found to decrease rapidly, and it was confirmed that the strain that may cause acne and inflammation in the skin can be effectively suppressed. At this time, when the concentration of phycocyanobilin was 0.5 or more, it could be confirmed that the concentration of both strains was significantly reduced, and when the concentration of phycocyanobilin was greater than or equal to 15, it was confirmed that the concentration reduction range of the strain was saturated.

<Experimental Example 3>

From Spirulina Phycocyanobilin Acne improvement / sebum secretion reduction / irritation of the contained composition

In order to measure the presence or absence of acne improvement, sebum secretion, and irritation of the composition of the present invention prepared in Example 1, 15 people with acne were allowed to use the prepared cosmetic composition for one month. The acne improvement scale was set from 1 to 5 points, with 1 point as ‘No,’, 3 points as ‘normal’, and 5 points as ‘very yes.’

The experimental results were expressed as the average score of 12 people in the following [Table 4]. Acne disappearance time was based on the number of days that disappearance was read, and acne recurrence was based on the result after 1 month with or without acne. The reduction in sebum secretion was set from 1 to 5 points, 1 point was'no', 3 points were'normal', and 5 points were marked as'very yes'. The experimental results are expressed as the average score of 15 people in Table 4 below. The presence or absence of skin irritation was considered as (number of persons with irritation reaction)/(total number of test subjects). The results are shown in Table 3 below.

Acne improvement, sebum secretion and irritation according to the concentration of the composition containing phycocyanobilin from Spirulina Sample concentration (%) Acne improvement Reduction of sebum secretion Presence or absence of stimulation 0.1 3.1 4.0 0/15 0.5 4.2 4.4 0/15 One 3.3 3.6 2/15 2 3.5 3.8 1/15 5 4.8 4.2 2/15 10 4.6 4.0 2/15 15 4.6 3.8 2/15 20 4.4 3.6 4/15

As a result, as shown in [Table 3], when developing a cosmetic for improving acne, it was confirmed that it is effective in improving acne. In addition, it was confirmed that there was a partial decrease in sebum secretion, and it was confirmed that irritation appeared only at very high concentrations for skin irritation. By obtaining an average of 4 points or more for acne improvement and an average of 4 points or more for sebum secretion reduction, it can be seen that there is an excellent effect on acne improvement. In addition, as the most suitable concentration, when the concentration of the composition containing phycocyanobilin was about 5 to 10%, it was confirmed that the effect was excellent in improving acne, but it was found that skin irritation was intensified at more than 20%.

<Experimental Example 4>

From Spirulina Phycocyanobilin Measurement of the effect of improving atopic dermatitis of the composition containing (PCB)

In order to confirm the atopic dermatitis improvement effect of the present invention prepared in Example 1, 10 out of 20 adult men and women with atopic dermatitis were applied from Spirulina at a concentration of 1% of the composition containing Phycocyanobilin (PCB). , The remaining 10 people were applied with water as a control. It was tested for 10 days by applying 1 ml or 1 g twice a day on a cotton pad and applying it to the affected area. After 10 days of application, the improvement effect was quantified in four stages: very effect, slight effect, no effect, and worse.

Measurement result of atopic dermatitis improvement effect according to the concentration of composition containing phycocyanobilin from Spirulina division Atopic dermatitis opening effect Average (%) Water Very good 0 0 good 0 0 no effect 8 80 worse 2 20 Sample Very good 2 20 good 6 60 no effect 2 20 worse 0 0

As shown in [Table 4], when the composition containing phycocyanobilin from Spirulina was used as 1%, about 80% answered that it was effective, and it was confirmed that there is a significant effect on atopic dermatitis. Relatively, most of the control formulations using water were found to be ineffective. Therefore, it could be confirmed that the composition containing Phycocyanobilin (PCB) from Spirulina of the present invention is effective in atopic dermatitis when the composition is used in an amount of 1% or more.

<Experimental Example 5>

From Spirulina Phycocyanobilin Elastase activity of compositions containing (PCB) Inhibition Measure effectiveness

Porcine pancreas elastase (porcine pancreas elastase, Sigma Co., Ltd. USA) and N-substrate of the elastase activity inhibitory effect to confirm the wrinkle improvement effect by the composition of the present invention prepared in Example 1 above. It was investigated by enzymatic reaction using succinyl-(L-Ala)3-ρ-nitroanilide (Sigma Co., Ltd USA).

Add 0.5 ml of the solution of the composition to a test tube, add 0.5 ml of porcine pancreas elastase (1U/ml) solution dissolved in 100mM Tris-HCl buffer (pH 8.0), and then dissolve in DMSO to prepare 3.2μM N-succinyl-(L-Ala). )3-ρ-nitroanilide was added 0.1 ml each, mixed well, and reacted at room temperature for 20 minutes. Elastase activity was investigated by measuring the amount of ρ-nitroanilide produced from the substrate at 410 nm using a spectrophotometer. The inhibitory ability of elastase activity was expressed as the rate of decrease in absorbance between the addition and no addition of the sample solution.

The above results are shown in FIG. 2, and as a result, a relatively high elastase inhibitory effect was shown when the composition of the present invention was treated. Particularly, when the concentration of phycocyanobilin exceeded 5, a saturation phenomenon was observed. Therefore, it was more preferable to set the concentration to 10 or less.

<Experimental Example 6>

From Spirulina Phycocyanobilin Melanin synthesis inhibition activity measurement of a composition containing (PCB)

In order to confirm the skin whitening effect of the composition of the present invention prepared in Example 1 above, an activity test of melanin synthesis inhibition activity was performed. Clone-M3 cells, which are melanocytes derived from experimental mice, produce melanin.

Melanin is biosynthesized in the melanosome, an organelle in melanocytes in the base layer of the epidermis, which can be compared with the amount produced by measuring the absorbance in the visible region. After inoculation of CloneM-3 cells, the sample is treated for 3 days, the medium is removed, the cells are washed with PBS, 1 ml of 1N NaOH is added to each well, and the membrane is melted by stirring to dissolve the melanin component. Visible light The amount of production was compared by measuring absorbance at 400 nm, which is the blue visible light region with the shortest wavelength among the bands.

3 is a result of showing the melanin synthesis inhibition activity. In order to observe the effect on melanin biosynthesis and cell viability when a sample is administered using Clon-M3 cells, which are melanocytes, a composition containing Phycocyanobilin (PCB) from Spirulina was treated by concentration. After 24 hours, the rate of inhibition of melanogenesis was measured. As a result of the measurement, it was confirmed that the inhibition rate increased from 0.1% to 10%, and finally, the inhibition rate reached about 30.8%. At this time, preferably, it can be seen that the greatest inhibition rate is shown when the concentration of phycocyanobilin is 2 to 10, and when it exceeds 10, the increase in the inhibition rate is reduced.

<Experimental Example 7>

Measurement of anti-ultraviolet effect of composition containing Phycocyanobilin (PCB) from Spirulina

In order to measure the anti-ultraviolet effect for the composition of the present invention prepared through Example 1, the following solution was prepared to measure the anti-ultraviolet effect. First, dissolve 40 mg of paraphenylenediamine in 20 ml of ethyl alcohol and 80 ml of water in a 100 ml volumetric flask, and then add 40 mg of paraphenylenediamine and about 30 mg of octylmethoxycinnamate in a separate 100 ml volumetric flask. 30 mg of the inventive composition was dissolved. After exposing the prepared solution to ultraviolet rays, the change in the content of paraphenylenediamine in each solution was measured using high-performance liquid chromatography (HPLC), and the change in content was as follows until 8 hours after the start of the experiment at 2 hour intervals. Measured under the analysis conditions and shown in Table 6 below.

Measurement result of anti-ultraviolet effect of composition containing Phycocyanobilin (PCB) from Spirulina Sample Immediately after the experiment 2 hours elapsed 4 hours elapsed 6 hours elapsed 8 hours elapsed PPD 100% 70.26 56.88 40.12 30.49 PPD+CMC 100% 88.54 81.20 76.62 70.13 PPD+ composition 100% 90.33 84.70 80.04 75.55

As a result, as shown in [Table 5], it was confirmed that the composition containing Phycocyanobilin (PCB) from Spirulina had excellent anti-ultraviolet effects.

<Example 2>

From Spirulina Phycocyanobilin (PCB) of the containing composition Cosmetic Composition preparation

Softening lotion, nutritional lotion, nutritional cream, and pack containing the composition of the present invention prepared through Example 1 were prepared in the following component ratios.

Flexible lotion composition ratio ingredient Manufacturing Example 1
(Unit: wt%) Manufacturing Example 2
(Unit: wt%) PCB containing composition 0.4 10.0 glycerin 5.0 5.0 1,3-butylene glycol 3.0 3.0 REG 1500 1.0 1.0 Allantoin 0.1 0.1 DL-panthenol 0.3 0.3 EDTA-2Na 0.02 0.02 Benzophenone-9 0.04 0.04 Sodium hyaluronate 5.0 5.0 ethanol 10.0 10.0 Octyldodeces-16 0.2 0.2 Polysorbate 20 0.2 0.2 Uniside-U 13 0.01 0.01 Distilled water Balance Balance Sum 100 100

Flexible lotion composition ratio ingredient Manufacturing Example 3
(Unit: wt%) Manufacturing Example 4
(Unit: wt%) PCB containing composition 0.4 10.0 Glyceryl stearate SE 1.5 1.5 Cetearyl alcohol 1.5 1.5 lanolin 1.5 1.5 Polysorbate 60 1.3 1.3 Sorbitastearate 0.5 0.5 Hydrogenated vegetable oil 4.0 4.0 Mineral oil 5.0 5.0 Trioctanoine 2.0 2.0 Dimethicone 0.8 0.8 Tocopherol acetate 0.5 0.5 Carboxyvinyl polymer 0.12 0.12 glycerin 5.0 5.0 1,3-butylene glycol 3.0 3.0 Sodium hyaluronate 5.0 5.0 Triethanolamine 0.12 0.12 Uniside-U 13 0.02 0.02 Distilled water Balance Balance Sum 100 100

Nutritional cream composition ratio ingredient Manufacturing Example 5
(Unit: wt%) Manufacturing Example 6
(Unit: wt%) PCB containing composition 0.4 10.0 Glycerin monostearate 1.5 1.5 Cetearyl alcohol 1.5 1.5 Stearic acid 1.0 1.0 Polysorbate 60 1.5 1.5 Sorbitastearate 0.6 0.6 Isostearyl isosterate 5.0 5.0 Squalane 5.0 5.0 Mineral oil 35.0 35.0 Dimethicone 0.5 0.5 Hydroxyethyl cellulose 0.12 0.12 glycerin 6.0 6.0 Triethanolamine 0.7 0.7 Uniside-U13 0.02 0.02 Distilled water Balance Balance Sum 100 100

Pack composition ratio ingredient Manufacturing Example 7
(Unit: wt%) Manufacturing Example 8
(Unit: wt%) PCB containing composition 0.4 10.0 glycerin 10.0 10.0 Betaine 5.0 5.0 REG 1500 2.0 2.0 Allantoin 0.1 0.1 DL-panthenol 0.3 0.3 EDTA-2Na 0.02 0.02 Benzophenone-9 0.04 0.04 Hydroxyethyl cellulose 0.1 0.1 Sodium hyaluronate 80. 80. Carboxyvinyl polymer 0.2 0.2 Triethanolamine 0.18 0.18 Octyldodecanol 0.3 0.3 Octyldodeces-16 0.4 0.4 ethanol 6.0 6.0 Uniside-U13 0.01 0.01 Distilled water Balance Balance Sum 100 100

As described above, in the present specification and drawings, a preferred embodiment of the present invention has been disclosed, and although specific terms are used, this is only used in a general meaning to easily explain the technical content of the present invention and to aid understanding of the invention. , It is not intended to limit the scope of the present invention. In addition to the embodiments disclosed herein, it is apparent to those of ordinary skill in the art that other modified examples based on the technical idea of the present invention may be implemented.

Claims (13)

In the cosmetic composition,
Contains phycocyanobilin derived from spirulina and derivatives thereof as an active ingredient,
The cosmetic composition, characterized in that the phycocyanobilin and derivatives thereof are contained in an amount of 4 to 11% by weight based on the total weight of the cosmetic composition.
The method of claim 1,
The spirulina cosmetic composition comprising at least one selected from spirulina planters (spirulina platensis) or spirulina maxima (spirulina maxima).
The method of claim 1,
The phycocyanobilin is prepared by centrifuging an aqueous spirulina solution, adding TCA to the separated supernatant, stirring, and then concentrating and co-drying the reaction product obtained by adding MeOH to the precipitate.
The method of claim 1,
The cosmetic composition, characterized in that the phycocyanobilin and derivatives thereof are (3E)-PCB or (3Z)-PCB.
The method of claim 1,
The cosmetic composition is a cosmetic composition, characterized in that used to improve skin inflammatory diseases.
delete delete The method of claim 5,
The cosmetic composition, characterized in that the skin inflammatory disease is acne dermatitis or atopic dermatitis.
The method of claim 5,
The cosmetic composition is a cosmetic composition characterized in that it has a dermatitis improvement effect through antibacterial activity against Propionebacterium acne (Propionebacterium acne), which is a trigger of acne dermatitis, and Staphylococcus aureus, which is a pyogenic bacteria.
The method of claim 1,
The cosmetic composition is a cosmetic composition, characterized in that used to improve skin wrinkles and skin whitening.
delete The method of claim 1,
The cosmetic composition is a cosmetic composition, characterized in that used for anti-ultraviolet purposes
The method of claim 1,
The cosmetic composition is skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, soap, shampoo, cleansing A cosmetic composition, characterized in that it is formulated with any one selected from the group consisting of foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, lipstick, makeup base, foundation, press powder, and loose powder.

Images