KR102431443B1 - Composition for improving skin wrinkles and elasticity containing the extract of Hibiseu Smanihot L. - Google Patents

Abstract

The present invention relates to a composition for improving skin wrinkles and elasticity comprising a Geumhwagyu extract, wherein the Geumhwagyu extract according to the present invention has an excellent effect of promoting collagen production in skin fibroblasts, and is useful for improving skin wrinkles and elasticity When using a mixture of Geumhwagyu root extract and radish extract, there is a synergistic effect in improving skin wrinkles and elasticity.

Description

Composition for improving skin wrinkles and elasticity containing the extract of Hibiseu Smanihot L.}

The present invention relates to a composition for improving skin wrinkles and elasticity, comprising an extract of Geumhwagyu.

Cosmetics are deeply related to our daily life and are used by many people. Before the development of human civilization, the purpose of makeup was to protect the body from nature, but with the development of science and civilization, the purpose of makeup is to clean and beautify the body and delay aging to lead a beautiful and comfortable life. changed The functions of cosmetics are not only cleanliness and aesthetic functions, but also free radicals, free radicals, free radicals, and peroxides caused by external factors such as pollutants, stress, and ultraviolet rays, and internal factors such as decreased immune cell function and cell activity. It is to remove or inhibit the causes of skin aging, such as destruction of proteins, nucleic acids, and cell membrane lipids caused by such factors. In addition, cosmetics having whitening, skin wrinkle improvement and suppression, and UV blocking functions are continuously being developed to satisfy consumers' desire for high-functional cosmetics. Among them, anti-wrinkle and anti-wrinkle products are receiving a lot of attention from consumers who want to maintain their youthful appearance.

Wrinkles caused by aging can be defined as bones that occur on the skin surface due to the loss of collagen and elastic fibers in the dermal layer of the skin. Skin wrinkles are determined by collagen collagen fibers present in the dermal layer, and collagen molecules are produced in fibroblasts and secreted into the dermal layer, and then formed into collagen fibers by auto-association (Implant dentistry. 2002. 11(3):280). -285). Collagen fibers are involved in the mechanical firmness of the skin in the dermal layer of the skin, the binding force of tissues, adhesion to cells and cell differentiation, etc. (Journal of the American Academy of Dermatology, 2001, 17(4):610-613). When the production of such collagen is reduced by internal factors and external factors or deformation is continuously accumulated, it causes wrinkle formation (Clinics in geriatric medicine, 2001, 17(4):617-630).

Collagen shows a quantitative decrease with increasing age and exposure to UV rays. Collagen is classified according to its morphological and structural characteristics, of which the most well-known are types I, II, III and IV. Collagen types I and III constitute a component of the intercellular matrix of the dermal layer, and collagen type IV is a major component of DEJ (Dermal Epidermal Junction). DEJ is located between the epidermal and dermal layers of the skin and controls the permeation and transport of substances between the two layers, controls the differentiation of adjacent skin cells, and maintains the firmness of the skin. Collagen and elastin, which are the main structural components of the skin, are denatured or biosynthesized by natural or external stimuli, which accelerates skin aging. In addition, the expression level and activity of collagenase is also a major factor in accelerating skin aging.

As a collagen synthesis promoting material, retinoic acid, epidermal growth factor (EGF), trans forming growth factor (TGF); Cardinale G. et al., Adv. Enzymol., 41, p. 425, 1974), an animal placental-derived protein (JP8-231370), betulinic acid (JP8-208424), chlorella extract, and the like are known. In particular, epidermal growth factor acts as a strong promoter of epithelial cell, endothelial and fibroblast cell proliferation, and has excellent wound healing effect because it promotes epithelial cell migration and proliferation in case of epithelial cell defect. In addition, retinol is very effective in inhibiting collagen synthesis and degradation, and the effect of improving skin wrinkles is specified in US Patent Nos. 4,603,146 and 4,877,805. However, in the case of retinoic acid, it is unstable, and when applied to the skin, there is a limit on the amount of use due to safety problems such as irritation and redness, and chlorella extract, etc., have insignificant effect, so it can not be expected to improve skin function by actually promoting collagen synthesis in the skin. Therefore, there is an urgent need to develop a new collagen synthesis promoter that is safe for the living body and has a higher effect than the existing collagen synthesis promoting material.

On the other hand, Geumhwagyu ( Hibiseu Smanihot L. ) is a rare medicinal plant, an annual herbaceous plant, also called wild bouillon. It is said that the flowers and leaves of Geumhwagyu, as well as the flower stalks and roots, are used to treat various malignant pains, such as antipyretic, detoxifying, anti-inflammatory, and analgesic effects. It is also rich in natural phytoestrogens, which are known to help reduce or prolong menopause syndrome.

However, to date, there has been no study on the collagen production enhancing effect of Geumhwagyu.

Laid-Open Patent Publication No. 10-2018-0004367

It is an object of the present invention to provide a cosmetic composition for improving skin wrinkles.

An object of the present invention is to provide a cosmetic composition for improving skin elasticity.

An object of the present invention is to provide a health food composition for improving skin wrinkles.

An object of the present invention is to provide a health food composition for improving skin elasticity.

It is an object of the present invention to provide a health functional food composition for improving skin wrinkles.

An object of the present invention is to provide a health functional food composition for improving skin elasticity.

In order to achieve the above object,

The present invention provides a cosmetic composition for improving skin wrinkles comprising an extract of Geumhwagyu ( Hibiseu Smanihot L. ).

The present invention provides a cosmetic composition for improving skin elasticity comprising an extract of Geumhwagyu ( Hibiseu Smanihot L. ).

The present invention provides a health food composition for improving skin wrinkles comprising an extract of Geumhwagyu ( Hibiseu Smanihot L. ).

The present invention provides a health food composition for improving skin elasticity comprising an extract of Geumhwagyu ( Hibiseu Smanihot L. ).

The present invention provides a health functional food composition for improving skin wrinkles comprising an extract of Geumhwagyu ( Hibiseu Smanihot L. ).

The present invention provides a health functional food composition for improving skin elasticity comprising an extract of Geumhwagyu ( Hibiseu Smanihot L. ).

In the composition according to the present invention, the Geumhwagyu extract is water, lower alcohol having 1 to 4 carbon atoms, butylene glycol, propylene glycol, glycerin, hexane, methylene chloride, ethyl acetate, n-butanol, etc. alone as an extraction solvent Or two or more types may be mixed and used. Preferably, ethanol may be used as an extraction solvent at a concentration of 100% or diluted with an aqueous ethanol solution of various concentrations.

Geumhwagyu ( Hibiseu Smanihot L. ) extract according to the present invention has an excellent effect of promoting collagen production in skin fibroblasts, and has a useful effect in improving skin wrinkles and elasticity, and can be used by mixing the Geumhwagyu root extract and radish extract It has a synergistic effect in improving skin wrinkles and elasticity.

1 is a result of evaluating the cytotoxicity in human skin fibroblasts (Hs68 cell) according to the extraction site, extraction solvent, and treatment concentration of a Geumhwagyu extract.
2 is a result of quantitative evaluation of collagen production-related gene expression in human skin fibroblasts (Hs68 cells) according to the treatment with Geumhwagyu extract through real-time PCR (****p<0.0001, ***p <0.0005, **p<0.005, *p<0.05).
3 is a result of quantitative evaluation through real-time PCR of the expression levels of collagen-degrading proteins (MMP1, MMP14) in human skin fibroblasts (Hs68 cells) according to the treatment with Geumhwagyu extract (****p<0.0001, ***p<0.0005, **p<0.005, *p<0.05).
Figure 4 (A) is a result of evaluating the amount of collagen production in human dermal fibroblasts (Hs68 cells) according to the treatment with Geumhwagyu extract (*p<0.05).
Figure 4 (B) is the result of confirming the expression level of the COL1A1 gene in human skin fibroblasts (Hs68 cells) according to the treatment with Geumhwagyu extract by western blotting.
5 is a result of confirming the expression level of the COL1A1 gene in human skin fibroblasts (Hs68 cells) according to the treatment of Geumhwagyu root and radish extract by western blotting.

Hereinafter, the present invention will be described in detail.

cosmetic composition

The present invention provides a cosmetic composition comprising an active substance.

The cosmetic composition is, for example, a solution, a gel, a solid or kneaded anhydrous product, an emulsion obtained by dispersing an oil phase in an aqueous phase, a suspension, a microemulsion, a microcapsule, a microgranule or an ionic (liposome), a nonionic vesicle dispersant It may be provided in the form of a cream, toner, lotion, powder, ointment, spray or concealer stick. In addition, it may be prepared in the form of a foam or an aerosol composition further containing a compressed propellant.

In addition, the cosmetic composition comprises a fatty substance, an organic solvent, a solubilizer, a thickening agent and a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, Commonly used in water, ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or cosmetics It may contain adjuvants commonly used in the cosmetic field, such as any other ingredients that are used.

In the cosmetic composition containing the active substance of the present invention, the active substance of the present invention may be added in an amount of 0.1 to 50% by weight, preferably 1 to 10% by weight, to the cosmetic composition usually contained.

When the active substance of the present invention is used as an external preparation for skin, additionally, a fatty substance, an organic solvent, a solubilizer, a thickening agent and a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant , water, ionic or non-ionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or for external use on the skin It may contain adjuvants commonly used in the field of dermatology, such as any other ingredients used as a skin care product. In addition, the above ingredients may be introduced in an amount generally used in the field of dermatology.

Health food and health functional food composition

There are no special restrictions on the type of food. Examples of foods to which the active substance of the present invention can be added include drinks, meat, sausage, bread, biscuits, rice cakes, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, There are various soups, beverages, alcoholic beverages and vitamin complexes, dairy products and processed dairy products, and includes all health food and health functional food in the ordinary sense.

The health food and health functional food composition containing the active substance according to the present invention may be added to food as it is or used together with other food or food ingredients, and may be appropriately used according to a conventional method. The mixing amount of the active substance may be appropriately determined depending on the purpose of its use (for prevention or improvement). In general, the amount of the composition in health food and health functional food may be added in an amount of 0.1 to 90 parts by weight based on the total weight of the food. However, in the case of long-term intake for health maintenance or health control, the above amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range.

The health food and health functional food composition of the present invention is not particularly limited in other ingredients except that it contains the active substance of the present invention as an essential ingredient in the indicated ratio, and various flavoring agents or natural carbohydrates are added as additional ingredients like conventional beverages. may contain. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; disaccharides such as maltose, sucrose and the like; and polysaccharides such as conventional sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (taumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g, per 100 nutraceuticals of the present invention.

In addition to the above, health food and health functional food composition containing the active substance of the present invention are various nutrients, vitamins, minerals (electrolytes), synthetic flavoring agents and flavoring agents such as natural flavoring agents, coloring agents and thickening agents (cheese, chocolate, etc.) ), pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, the health food and health functional food composition of the present invention may contain natural fruit juice, fruit juice, and fruit for the production of a vegetable drink.

These components may be used independently or in combination. The proportion of these additives is not so important, but is generally selected in the range of 0.1 to about 20 parts by weight per 100 parts by weight of the health food and health functional food composition containing the active substance of the present invention.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples only illustrate the present invention, and the content of the present invention is not limited by the following examples.

<Example 1> Geumhwagyu ( Hibiseu Smanihot L. ) preparation of extracts

Geumhwagyu grown in Korea was dried by roots, stems, and leaves, and then extracted with 100% ethanol or 70% ethanol as an extraction solvent. In the case of Geumhwagyu leaves, the yield was very low when extracted with 70% ethanol, and extraction was performed with 100% ethanol. The extract obtained above was freeze-dried and then dissolved in DMSO solvent to prepare samples of various concentrations (0.01, 0.1, 1, 10, 100 μg/mL) and used in the following experiments.

<Experimental Example 1> Geumhwagyu ( Hibiseu Smanihot L. ) evaluation of the cytotoxicity of the extract

Human skin fibroblasts (Hs68 cells) were treated with the sample prepared in Example 1 for each concentration for 24 hours, and then toxicity of the extract was evaluated in skin cells through CCK-8 assy, and the results are shown in FIG. 1 . Here, the control group (Cont) was not treated with the sample.

1 is a result of evaluating the cytotoxicity in human skin fibroblasts (Hs68 cells) according to the extraction site, extraction solvent and treatment concentration of Geumhwagyu extract.

As shown in FIG. 1 , no cytotoxicity was observed in any of the samples, and it was confirmed that the Geumhwagyu extract was safe for the skin.

<Experimental Example 2> Evaluation of the effect of Geumhwagyu extract on collagen gene expression

It occupies the largest portion of skin collagen, which increases the expression level of COL1A1 (Collagen, type I, alpha 1) gene, COL5A1 (Collagen, type V, alpha 1) gene, and COL6A1 (Collagen, type VI, alpha 1) gene expression. An experiment was conducted to find out.

Specifically, the effect of 10 μg/mL of the Geumhwagyu extract sample prepared in Example 1 on the expression of collagen genes after 24 hours of treatment with human skin fibroblasts (Hs68 cells) was confirmed through real-time PCR, and the The results are shown in FIG. 2 . Here, the control group (Cont) was not treated with the sample.

2 is a result of quantitative evaluation of collagen production-related gene expression in human skin fibroblasts (Hs68 cells) according to the treatment with Geumhwagyu extract through real-time PCR (****p<0.0001, ***p <0.0005, **p<0.005, *p<0.05).

As shown in Figure 2 (A), in the case of Geumhwagyu root and stem extracted with 100% EtOH, and Geumhwagyu root extract extracted with 70% EtOH, it occupies the most portion in skin collagen, which is COL1A1 It was confirmed that the gene expression of the control group was increased more than 2 times compared to the control group.

As shown in FIG. 2 (B), the Geumhwagyu root extract extracted with 100% EtOH and the Keumhwagyu root extract extracted with 70% EtOH seemed to slightly increase the gene expression of COL5A1 compared to the control group, but it was not statistically significant. It was found that there was no significant effect on the expression of the COL5A1 gene by all regions of Keumhwagyu.

As shown in Fig. 2 (C), it was confirmed that all extracts of each part of Geumhwagyu increased the expression of the COL6A1 gene.

Combining the results of FIG. 2 , it is expected that the Geumhwagyu root extract extracted with 100% EtOH solvent, the large extract, and the Geumhwagyu root extract extracted with the 70% EtOH solvent will promote collagen synthesis.

<Experimental Example 3> Evaluation of the effect of Geumhwagyu extract on MMP protein expression

MMP (matrix metalloproteinase) protein is a protein that decomposes collagen, and an experiment was conducted to investigate the effect of Geumhwagyu extract on the reduction of MMP gene expression.

Specifically, the effect on the expression of MMP1 and MMP14 genes after 24 hours of treatment with 10 μg/mL of the Geumhwagyu extract sample prepared in Example 1 in human skin fibroblasts (Hs68 cells) was confirmed through real-time PCR. , the results are shown in FIG. 3 . Here, the control group (Cont) was not treated with the sample.

3 is a result of quantitative evaluation through real-time PCR of the expression levels of collagen-degrading proteins (MMP1, MMP14) in human skin fibroblasts (Hs68 cells) according to the treatment with Geumhwagyu extract (****p<0.0001, ***p<0.0005, **p<0.005, *p<0.05).

As shown in Figure 3 (A), 100% EtOH root, large, 70% EtOH root extract significantly decreased the expression level of MMP1 protein, whereas in the case of 100% EtOH leaf extract, the expression level of MMP1 protein increased inversely. confirmed that.

As shown in FIG. 3 (B), in the case of Geumhwagyu root extract extracted with 70% EtOH, the expression level of MMP14 protein increased, resulting in negative results, but in all other experimental groups, there was a statistically significant difference in the expression of MMP14 protein. did not appear

3, it is expected that the 100% solvent-extracted Geumhwagyu root and large extracts reduce the expression of MMP1 and do not affect the expression of MMP14, so these two extracts are expected to inhibit collagen degradation. do.

<Experimental Example 4> Evaluation of collagen synthesis increase in Geumhwagyu extract

An experiment was conducted to find out whether Geumhwagyu extract actually increases collagen production in human skin fibroblasts.

Specifically, the amount of soluble collagen was measured using Sirius red staining method after 48 hours of treatment with 10 μg/mL of the Geumhwagyu extract sample prepared in Example 1 in human skin fibroblasts (Hs68 cells), and the results are shown in Fig. 4 (A) shows. In addition, the result of confirming the expression level of the COL1A1 gene by western blotting method after 48 hours of treatment with 10 μg/mL of the Geumhwagyu extract sample prepared in Example 1 in human skin fibroblasts (Hs68 cells) is shown in FIG. 4 (B). indicated.

Figure 4 (A) is a result of evaluating the amount of collagen production in human dermal fibroblasts (Hs68 cells) according to the treatment with Geumhwagyu extract (*p<0.05).

Figure 4 (B) is the result of confirming the expression level of the COL1A1 gene in human dermal fibroblasts (Hs68 cells) according to the treatment with Geumhwagyu extract by western blotting.

As shown in FIG. 4 (A), it was confirmed that the amount of soluble collagen was increased by about 3 times compared to the control in the case of Geumhwagyu root and soybean extract extracted with 100% EtOH.

As shown in FIG. 4 (B), it was confirmed that the COL1A1 gene expression level was increased in the case of Geumhwagyu root and soybean extracts extracted with 100% EtOH compared to the control group.

Combining the results of Figure 4, it was confirmed that the Geumhwagyu root and large extract extracted with 100% EtOH increased the COL1A1 gene expression and at the same time increased the overall soluble collagen synthesis.

<Experimental Example 5> Evaluation of collagen synthesis synergistic effect of Geumhwagyu root and radish mixed extract

After drying domestic organic radish ( Raphanus sativus var. hortensis for. Acanthiformis MAKINO ) and Geumhwagyu ( Hibiseu Smanihot L. ) roots, each was extracted with 100% ethanol (EtOH) solvent, and then each lyophilized extract was dissolved in DMSO solvent. After that, a mixed extract sample was prepared at the weight ratio specified in FIG. 5 .

The COL1A1 gene expression pattern was confirmed after treatment with the thus prepared mixed extract solution in human skin fibroblast Hs68 cells at a concentration of 10ug/ml for 24 hours, and the results are shown in FIG. 5 .

5 is a result of confirming the expression level of the COL1A1 gene in human dermal fibroblasts (Hs68 cells) according to the treatment of Geumhwagyu root and radish extract by western blotting.

As shown in FIG. 5 , a significant synergistic effect was selectively observed only in the sample in which the radish extract and the Geumhwagyu root extract were mixed in an 80:20 weight ratio, and no synergistic effect was observed in the sample mixed in different ratios.

On the other hand, unusually, such a synergistic effect was not observed in the mixed extract of the radish extract and the extract from other parts than the root of Geumhwagyu, which is expected to produce a synergistic effect between the specific component of the root of Geumhwagyu and the specific component of the radish.

Preparation example of cosmetics

The active substance according to the present invention can be manufactured into various types of cosmetics depending on the purpose. The following exemplifies the manufacturing method of several cosmetics containing the active ingredient according to the present invention as an active ingredient, but the present invention is not limited thereto.

<Cosmeceutical Preparation Example 1> Preparation of softened lotion

10.0 parts by weight of active substance

1,3-butylene glycol 1.00 parts by weight

Disodium EDTA 0.05 parts by weight

Allantoin 0.10 parts by weight

Dipotassium glycyrrhizate 0.05 parts by weight

citric acid 0.01 parts by weight

Sodium citrate 0.02 parts by weight

Glycereth-26 1.00 parts by weight

Arbutin 2.00 parts by weight

1.00 parts by weight of hydrogenated castor oil

Ethanol 30.0 parts by weight

preservative trace

trace amount of colorant

Trace amount of flavoring agent

Purified water remaining

<Cosmeceutical Preparation Example 2> Preparation of nourishing cream

10.0 parts by weight of active substance

1,3-butylene glycol 7.00 parts by weight

1.00 parts by weight of glycerin

D-panthenol 0.10 parts by weight

3.20 parts by weight of plant extract

0.30 parts by weight of magnesium aluminum silicate

PEG-40 stearate 1.20 parts by weight

2.00 parts by weight of stearic acid

1.50 parts by weight of polysorbate 60

2.00 parts by weight of lipophilic glyceryl stearate

1.50 parts by weight of sorbitan sesquioleate

3.00 parts by weight of cetearyl alcohol

4.00 parts by weight of mineral oil

3.80 parts by weight of squalane

2.80 parts by weight of carlylic/capric triglyceride

1.80 parts by weight of vegetable oil

Dimethicone 0.40 parts by weight

Trace amount of dipotassium glycyrrhizate

allantoin traces

Sodium Hyaluronate Trace

Tocopheryl Acetate Appropriate amount

Appropriate amount of triethanolamine

Appropriate amount of preservative

Appropriate amount of flavoring agent

Purified water remaining

Manufacturing example of health food

The active substance according to the present invention can be manufactured into various types of health food depending on the purpose. The following exemplifies the manufacturing method of several health foods containing the active ingredient according to the present invention as an active ingredient, but the present invention is not limited thereto.

<Health food production example 1> Production of dairy products

0.01-1 parts by weight of the active substance of the present invention was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

<Health food production example 2> Preparation of wire food

Brown rice, barley, glutinous rice, and barley radish were pregelatinized by a known method and dried, and then roasted and prepared as a powder having a particle size of 60 mesh with a grinder. Black soybeans, black sesame, and perilla were also steamed and dried by a known method, and then roasted and prepared into powder having a particle size of 60 mesh with a grinder. The active material of the present invention was concentrated under reduced pressure in a vacuum concentrator to obtain a dry powder. The above-prepared grains, seeds, and dry powders of active substances were prepared by blending them in the following ratios.

Grains (34 parts by weight of brown rice, 19 parts by weight of barley, 20 parts by weight of barley),

Seeds (7 parts by weight of perilla, 8 parts by weight of black beans, 7 parts by weight of black sesame),

active substance (2 parts by weight),

Reishi (1.5 parts by weight), and

Rehmannia (1.5 parts by weight).

Manufacturing example of health functional food

The active substance according to the present invention can be manufactured into various types of health functional food depending on the purpose. The following exemplifies the manufacturing method of several health functional foods containing the active ingredient according to the present invention as an active ingredient, but the present invention is not limited thereto.

<Health functional food manufacturing example 1> Manufacture of health functional food

100 mg of active substance

appropriate amount of vitamin mixture

Vitamin A Acetate 70 μg

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

0.5 mg of vitamin B6

0.2 μg of vitamin B12

Vitamin C 10 mg

Biotin 10 μg

Nicotinamide 1.7 mg

50 μg of folic acid

Calcium pantothenate 0.5 mg

Appropriate amount of inorganic mixture

ferrous sulfate 1.75 mg

Zinc Oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Dibasic calcium phosphate 55 mg

Potassium citrate 90 mg

100 mg calcium carbonate

Magnesium chloride 24.8 mg

Although the composition ratio of the vitamin and mineral mixture is relatively suitable for health functional food in a preferred embodiment, the composition ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health functional food manufacturing method. Then, the granules can be prepared and used in the preparation of a health functional food composition according to a conventional method.

<Health functional food production example 2> Preparation of health functional beverage

100 mg of active substance

100 mg citric acid

100 mg of oligosaccharides

Plum Concentrate 2 mg

Taurine 100 mg

Add purified water to total 500 mL

After mixing the above ingredients according to a conventional health drink manufacturing method, stirring and heating at 85° C. for about 1 hour, the resulting solution is filtered and obtained in one sterilized container, sealed and sterilized, and then refrigerated. used in the manufacture of health beverage compositions of Although the composition ratio is prepared by mixing ingredients suitable for relatively favorite beverages in a preferred embodiment, the mixing ratio may be arbitrarily modified according to regional and national preferences such as demand class, demand country, and use purpose.

Claims (18)

Geumhwagyu ( Hibiseu Smanihot L. ) root extract and radish ( Raphanus sativus var. hortensis for. acanthiformis MAKINO ) extract,
A cosmetic composition for improving skin wrinkles, comprising 20 parts by weight of the Geumhwagyu root extract based on 80 parts by weight of the radish extract.
delete The method of claim 1,
The extract is water, a lower alcohol having 1 to 4 carbon atoms, butylene glycol, propylene glycol, glycerin, hexane, methylene chloride, ethyl acetate and n-butanol, characterized in that obtained using one or more extraction solvents, skin A cosmetic composition for improving wrinkles.
Geumhwagyu ( Hibiseu Smanihot L. ) root extract and radish ( Raphanus sativus var. hortensis for. acanthiformis MAKINO ) extract,
A cosmetic composition for improving skin elasticity, comprising 20 parts by weight of the Geumhwagyu root extract based on 80 parts by weight of the radish extract.
delete 5. The method of claim 4,
The extract is water, a lower alcohol having 1 to 4 carbon atoms, butylene glycol, propylene glycol, glycerin, hexane, methylene chloride, ethyl acetate and n-butanol, characterized in that obtained using one or more extraction solvents, skin A cosmetic composition for improving elasticity.
Geumhwagyu ( Hibiseu Smanihot L. ) root extract and radish ( Raphanus sativus var. hortensis for. acanthiformis MAKINO ) extract,
A health food composition for improving skin wrinkles, comprising 20 parts by weight of the Geumhwagyu root extract based on 80 parts by weight of the radish extract.
delete 8. The method of claim 7,
The extract is water, a lower alcohol having 1 to 4 carbon atoms, butylene glycol, propylene glycol, glycerin, hexane, methylene chloride, ethyl acetate and n-butanol, characterized in that obtained using one or more extraction solvents, skin A health food composition for wrinkle improvement.
Geumhwagyu ( Hibiseu Smanihot L. ) root extract and radish ( Raphanus sativus var. hortensis for. acanthiformis MAKINO ) extract,
A health food composition for improving skin elasticity, comprising 20 parts by weight of the Geumhwagyu root extract based on 80 parts by weight of the radish extract.
delete 11. The method of claim 10,
The extract is water, a lower alcohol having 1 to 4 carbon atoms, butylene glycol, propylene glycol, glycerin, hexane, methylene chloride, ethyl acetate and n-butanol, characterized in that obtained using one or more extraction solvents, skin A health food composition for improving elasticity.
Geumhwagyu ( Hibiseu Smanihot L. ) root extract and radish ( Raphanus sativus var. hortensis for. acanthiformis MAKINO ) extract,
A health functional food composition for improving skin wrinkles, characterized in that it contains 20 parts by weight of the Geumhwagyu root extract based on 80 parts by weight of the radish extract.
delete 14. The method of claim 13,
The extract is water, a lower alcohol having 1 to 4 carbon atoms, butylene glycol, propylene glycol, glycerin, hexane, methylene chloride, ethyl acetate and n-butanol, characterized in that obtained using one or more extraction solvents, skin A health functional food composition for wrinkle improvement.
Geumhwagyu ( Hibiseu Smanihot L. ) root extract and radish ( Raphanus sativus var. hortensis for. acanthiformis MAKINO ) extract,
A health functional food composition for improving skin elasticity, comprising 20 parts by weight of the Geumhwagyu root extract based on 80 parts by weight of the radish extract.
delete 17. The method of claim 16,
The extract is water, a lower alcohol having 1 to 4 carbon atoms, butylene glycol, propylene glycol, glycerin, hexane, methylene chloride, ethyl acetate and n-butanol, characterized in that obtained using one or more extraction solvents, skin A health functional food composition for improving elasticity.

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