KR101872340B1 - Skin whitening composition comprising Achyranthesjaponica Nakai extracts - Google Patents

Abstract

The present invention relates to a composition for skin whitening wherein an Achilleanthes japonica Nakai extract is further mixed with an Allium plant extract or a Poaceae plant extract. More particularly, the present invention relates to a composition for skin whitening, which contains an extract of Achyranthes japonica Nakai and an extract of Allium or Poaceae as an active ingredient and is excellent in tyrosinase and L-DOPA inhibitory effect.

Description

[0001] The present invention relates to a skin whitening composition comprising Achyranthes japonica Nakai extracts,

The present invention relates to a composition for skin whitening which is excellent as an inhibitor of tyrosinase and L-DOPA by including Achyranthes japonica Nakai as an active ingredient.

Environmental pollution caused by industrialization causes obstacles in general life. In particular, interest in skin related cosmetic and dermatological treatment materials is increasing due to changes in perception that not only the skin diseases increase but also the quality of life is improved as a body protection film. In general, with increasing environmental pollution, the preference for white skin is increasing due to skin damage due to ultraviolet rays, and there is a growing interest in a method for achieving a skin whitening effect in pursuit of clearer and cleaner skin by transparent make-up .

In addition, as the diversity and individualization of modern values have become prominent, the increase in interest has led to the development of functional cosmetics containing ingredients such as whitening, moisturizing, cell regeneration, and wrinkle improvement, and the cosmetics industry Research and development on natural materials are actively under way.

The epidermis, which is located at the outermost part of the body, is arranged in the order of the base layer, the polar layer, and the stratum corneum. Among the various cell types constituting it, melanocyte (melanocyte) is a biopolymer of phenol that determines hair color and skin color. It synthesizes melanin which is a complex of black protein and secretes it to prevent skin damage by ultraviolet But also the absorption of toxic drugs. However, excessive production of melanin causes skin diseases such as spots and freckles, and it is reported that it is also related to the induction of skin cancer in severe cases.

In general, skin color is determined by the content and distribution of melanin, and is related to the number and distribution of melanosomes released from the melanocytes and released to the outside of the cells. Melanin is produced in the organelle of melanocytes called melanosomes of skin cells and moves to epidermal cells called keratinocytes. Melanin is produced by oxidizing tyrosine by tyrosinase, which oxidizes tyrosine with tyrosine as a substrate. The hyperpigmentation of the skin can be caused by various factors such as hormonal disorders, genetic diseases, and ultraviolet irradiation after the inflammation reaction of the skin, and the main factor is the abnormal synthesis and distribution of melanin pigment.

From the viewpoint of importance of aesthetic function, studies on various materials for whitening by controlling the activity and expression of tyrosinase enzyme have been conducted, and hydroquinone, ascorbic acid, Kojic acid, retinole, arbutin, and the like.

However, most of the materials exhibiting a strong melanin biosynthesis inhibiting activity cause degeneration or lethality of the pigment cells and cause side effects such as impairing the inherent functions of the cells.

On the other hand, wax is a perennial plant belonging to Amaranthaceae, distributed in Korea, China, and Japan. It is known to be effective for arthritis in the private sector. Origin plants differ from country to country, using Achyranthes japonica Nakai for domestic wastes and Achyranthesbidentata Blume, Cyathula officinalis Kuan and Cyathulacapitata Moq in China (Kor J. Herbology 2007 22: 71-79, Kim et al.). However, in the current pharmacopoeia of Korea, two kinds of toxin, which are naturally occurring tobacco and Chinese origin, are defined as wax. It has been reported that there is no cytotoxicity and genotoxicity (13 weeks of repeated administration and genotoxicity test, Korea Research Institute for Chemical Technology, 2007) The development and commercialization of processed foods has been almost non-existent.

It is known that the womb in one room has the effect of lowering the efficacy and heat to generate blood, improve blood circulation, eliminate bitterness and sourness, functioning kidneys and liver, Journal of Gynecology, 2005. 18: 110-126, Kim, Kyung-Su and others). The main pharmacological components of wheat are oleanolic acid, inocosterone, ecdysterone, beta-sitosterol, stigmasterol and feruloyl tyramine glycoside (Chin, J., Nat. In recent years, research on the toxin has been carried out, and recently, it has been turned into a search for a useful physiological activity for efficient use of astaxanthin. The reported physiological activity is antioxidant activity (Kor. Herbology 2007. 22: 155-167, Park et al.), Antibacterial and anti-malarial activity (Korean J. Biotechnol. Bioeng 2002. 17: 537-542, Cai et al.), Anti-inflammatory activity, heavy metal removal and detoxification effect (Kor. J. Oriental Physiol. Pathol 2004. 18: 1784-1794, Kang et al.), Arthritis relief and hard tissue regeneration effect (The J. Appl. Pharmcol. 2002. 10: 253-257, Kim et al.), (Kor, J. Herbology 2012. 27: 77-83, Oh et al.), The effect of improving blood flow (see, for example, J. Orient Obster Gynecol, J. Tradit. Chin. Med. 2001. 21: 225-231, Xie et al.).

Allium hookeri is a plant belonging to the genus Allium and is also called root leek. It is a useful vegetable which is used in medicinal fields such as India, Bhutan, Southwest of Myanmar, . Allium plants which have been used for food and medicine for a long time in Korea include onion, leek, garlic, soleae, and persimmon. Allium spp., Which is used for daily dietary life, has been shown not only antioxidant activity and harmful oxygen scavenging activity but also lipid peroxidation inhibitory effect. It is called "三 菜" because it has sweetness, bitter taste and spicy taste. It is also called "玄 菜" because it has ginseng flavor. It is also used to study the quality characteristics and storage stability of beef patty, Studies on the preparation conditions of bread with triangular flour, the quality characteristics of kimchi with triplex root, and the inhibitory effect of triangular root extract on melanin formation have been carried out. However, studies on the identification of physiological activities and commercialization of functional products have not been conducted yet.

The root of reed is also called obi. Nogeun is a rhizome of the reed Phragmites communis Trin. (Gramineae), a tall perennial plant. The roots of the reeds are rough, large in length, stretch horizontally, have many beard roots in the nodes and are yellowish white. The stem is long, columnar, rigid, gathered, straight and 1-3 m high, with 2 rows of leaves, narrow and fulvic, 50 cm long, 4 cm wide, pointed with sharp edges, green with rough edges. The leaf has no hair, the tongue is short, the edge is hairy, and the flower is bloomed in September, bloomed in September, 15-50 cm in length, with a small number of small numbers attached closely. Few flowers have 5 flowers, and they are thin, long and sharp. The spines are 2 pieces, the length is different, and the fur of guns is long (1, 2). The dried squid is flat, cylindrical, glossy on the surface and yellowish white. The nodule is comparatively hard and bright yellow, with vertical wrinkles between the nodes. (3) It is removed in late spring or early summer or autumn to remove the clay and beard roots. Peel off the coating and use it when it is fresh or by drying it in the sun. It has been used to treat nausea due to fever, esophageal cancer, lung abscesses and to decipher the blowfish poison. (2004) Jang et al. (4) Because it contains coixol and vitamin C, it protects against ultraviolet rays and promotes the proliferation of melanocytes, so that the hair is black and shiny. When used as a whitening cream, .

Accordingly, the present inventors have made efforts to develop a skin whitening composition that minimizes adverse effects and improved whitening function. As a result, the present inventors have found that the extracts of raspberry extract, truffle extract, or truffle extract and reed root extract inhibit tyrosinase and L-DOPA And that the extract can be used as a composition for skin whitening, thereby completing the present invention.

It is an object of the present invention to provide a composition capable of inhibiting tyrosinase and L-DOPA by using the extract of Aspergillus oryzae, the extract of Aspergillus oryzae, and the extract of Gramineae.

Another object of the present invention is to provide a composition for skin whitening through inhibition of tyrosinase and L-DOPA by the extract of succulent extract and allium plant or the extract of astaxanthin and the extract of Ganoderma lucidum.

However, the technical problem to be solved by the present invention is not limited to the above-mentioned problems, and other matters not mentioned can be clearly understood by those skilled in the art from the following description.

To achieve the above object, according to the present invention, there is provided a composition for skin whitening comprising a water extract and an allium plant extract.

Wherein the extract of Allium plant contains at least one plant extract selected from the group consisting of soya bean, dumpling leek, garlic, mustard, onion, persimmon and tripe.

Preferably, the composition for skin whitening is characterized in that the allium plant extract is a triple-skin.

The composition of the present invention is a mixture of a raspberry extract and a mulberry extract at a weight ratio of 1:10 to 10: 1, preferably a raspberry extract and a mulberry extract at a weight ratio of 1: 1.

Also provided is a composition for skin whitening comprising a raspberry extract and a truffle extract.

The pine plant extract may be at least one plant extract selected from the group consisting of reeds, reed roots, giant roe deer, sorghum, barley, malt, oats, brown rice, corn, sorghum, yulmu, millet, rye and wheat A composition for skin whitening is provided.

Preferably, the pomegranate plant extract is a reed, and more preferably, the pomegranate plant extract is a reed root.

The composition of the present invention is a mixture of a wheat extract and a reed root extract at a weight ratio of 1:10 to 10: 1, preferably a wheat extract and a reed root extract at a weight ratio of 1: 1.

In another embodiment of the present invention, the composition according to the present invention can exhibit melanogenesis inhibitory effect by inhibiting tyrosinase and L-DOPA in the skin, and thus can be used as a skin whitening composition.

Further, in another embodiment of the present invention, the composition according to the present invention can be used as a skin whitening cosmetic composition. Herein, the cosmetic composition may be used in cosmetics, nutrition lotion, nutrition essence, massage cream, cosmetic bath additive, body lotion, body milk, bass oil, baby oil, baby powder, shower gel, shower cream, sunscreen lotion, Face and body lotions, Face and Body creams, Skin whitening creams, Hand lotions, Hair lotions, Cosmetic creams, Jasmine oils, Bath soaps, Skin creams, Skin lotions, Skin creams, Sunscreen cosmetics, , Soap, soap, shampoo, hand cleanser, medicinal soap medicine, cream soap, facial wash, whole body cleanser, scalp cleanser, hair rinse, cosmetic soap, tooth whitening gel, toothpaste . To this end, the composition of the present invention may further comprise a solvent commonly used in the production of a cosmetic composition, or a suitable carrier, excipient or diluent.

For example, water, saline solution, DMSO, or a combination thereof may be used. Examples of the carrier, excipient or diluent include purified water, oil, wax But are not limited to, fatty acids, fatty acid alcohols, fatty acid esters, surfactants, humectants, thickeners, antioxidants, viscosity stabilizers, chelating agents, buffers, lower alcohols and the like. Further, if necessary, it may contain a whitening agent, a moisturizing agent, a vitamin, an ultraviolet screening agent, a perfume, a dye, an antibiotic, an antibacterial agent, and an antifungal agent.

As the oil, hydrogenated vegetable oil, castor oil, cottonseed oil, olive oil, palm oil, jojoba oil and avocado oil may be used. Examples of the wax include wax, wax, carnauba, candelilla, montan, ceresin, liquid paraffin, Can be used.

As the fatty acid, stearic acid, linoleic acid, linolenic acid and oleic acid may be used. As the fatty acid alcohol, cetyl alcohol, octyldodecanol, oleyl alcohol, panthenol, lanolin alcohol, stearyl alcohol and hexadecanol may be used As fatty acid esters, isopropyl myristate, isopropyl palmitate, and butyl stearate may be used. As the surfactant, a cationic surfactant, an anionic surfactant and a nonionic surfactant known in the art can be used, and a surfactant derived from a natural material is preferably used as far as possible.

In addition, it may contain a hygroscopic agent, a thickening agent, an antioxidant and the like widely known in the field of cosmetics, and the kind and amount thereof are well known in the art.

In another embodiment of the present invention, the composition according to the present invention can be used as a food composition for skin whitening. The food composition may be prepared in the form of various foods such as beverages, gums, tea, vitamin complexes, powders, granules, tablets, capsules, confectionery, rice cakes and breads. Since the food composition of the present invention is composed of a plant extract having little toxicity and side effects, it can be safely used for prolonged use even for prophylactic purposes.

When the compound of the present invention is contained in the food composition, the amount thereof may be added in a proportion of 0.1 to 50% of the total weight.

Here, when the food composition is prepared in a beverage form, there are no particular limitations other than those containing the food composition at the indicated ratios and may contain various flavors or natural carbohydrates such as ordinary beverages as an additional ingredient. That is, natural carbohydrates include monosaccharides such as glucose, disaccharides such as fructose, sucrose and the like and sugar sugars such as polysaccharide, dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol can do. Examples of the above-mentioned flavors include natural flavors (such as tau martin and stevia extract (for example, rebaudioside A and glycyrrhizin) and synthetic flavors (for example, saccharine and aspartame).

In addition, the food composition of the present invention can be used as a flavoring agent such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, colorants, pectic acid and its salts, alginic acid and its salts, , a pH adjusting agent, a stabilizer, a preservative, a glycerin, an alcohol, a carbonating agent used in a carbonated drink, and the like.

These components may be used independently or in combination. The proportion of these additives is not a key element of the present invention, but is generally selected in the range of 0.1 to about 50 parts by weight per 100 parts by weight of the food composition of the present invention.

According to the present invention, the extracts of succulent and allium plants or the extracts of raspberry and rice plants and plant extracts inhibit tyrosinase and L-DOPA activity and inhibit melanin production in melanin-producing cells, , Cosmetics for the prevention, improvement and treatment of skin whitening and skin pigmentation diseases, pharmacy, external preparation for skin, quasi-drugs, food composition.

FIG. 1 shows the degree of inhibition of tyrosinase according to an embodiment of the present invention, with respect to the extracts of ryegrass, mugwort, and reed root.
FIG. 2 shows the synergistic effects of the tyrosinase inhibitory activity and the inhibitory activity on the tyrosinase inhibitory activity and the tyrosinase inhibitory activity, respectively, of the extract of Wasserliaceae and Thunb.
FIG. 3 shows the L-DOPA inhibition of each of the raspberry extract, tubercle leaf extract and reed root extract according to an embodiment of the present invention.
FIG. 4 shows the synergistic effects on the L-DOPA inhibition and inhibitory activity of the waxy and tubular extracts, the mussel and the reed root extract according to an embodiment of the present invention.

Hereinafter, the present invention will be described in more detail with reference to Examples. It will be apparent to those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

[ Manufacturing example ] Sample preparation

The extracts of ryegrass, tsutsuga and reed roots were lyophilized and used as extracts after pulverization.

[ Example  1] Measurement of tyrosinase inhibitory activity

In Example 1, tyrosinase inhibitory activity of each of the wax, triplicate and reed roots was measured. Tyrosinase inhibitory activity was measured by Mason and Peterson method. 20 μL (1 mg / mL) and a mushroom tyrosinase (500 units / mL, Sigma Co., St. Louis, MO) were added to 120 μL of 0.1 M potassium phophate buffer (pH 6.8). Louis, MO, USA) was added, and 40 μL of 1.5 mM tyrosin was added thereto, followed by reaction at 37 for 10 minutes. After the reaction, absorbance was measured at 450 nm and tyrosinase inhibitory activity was calculated as follows.

(%) = [{(A-B) - (C-D)} / (A-B)] 100

A: value of absorbance at 450 nm in the case of addition of tyrosinase and no sample added

B: value of absorbance at 450 nm in the absence of sample and tyrosinase

C: value of absorbance at 450 nm in the presence of sample and tyrosinase

 D: value of absorbance at 450 nm in the absence of tyrosinase, sample added

Fig. 1 and Table 1 show the results of inhibition of tyrosinase inhibition of each of the wax, triplex and reed roots.

sample The tyrosinase inhibition (inhibition rate at 1 mg / ml, % ) The 32.0 ± 0.7 Triptych 25.3 + 4.7 Reed roots 29.8 ± 4.2

[ Example  2] Synergistic Evaluation of Tyrosinase Inhibitory Activity

In Example 2, the synergistic evaluation of tyrosinase inhibitory activity of wisteria, thrush, wilt and reed roots was carried out on the basis of wax. (1 mg / ml concentration) and 1: 1 ratio (1 mg / ml concentration) of wastes to reed roots in the same manner as in Example 1 to prepare samples to measure tyrosinase inhibitory activity. The synergy effect was calculated by the following formula.

Synergistic efficacy increase rate (%) = {(inhibition rate% - predicted inhibition rate%) / predicted inhibition rate%} × 100

The expected inhibition rate was calculated as follows:

Expected inhibition rate of A extract + B extract = ((Inhibition rate of A obtained in Example 1) + (Inhibition rate of B obtained in example 1)) / 2

2 and Table 2 show the results of the synergistic effect on tyrosinase inhibitory activity and tyrosinase inhibitory activity of the wax, triplet, wilt and reed root, respectively.

sample Estimated inhibition rate ( % ) Inhibition rate % ) Synergistic Efficacy Growth Rate ( % ) The + Triptych (1: 1) 28.7 39.6 ± 0.8 38.2 The + Reed roots (1: 1) 30.9 42.9 ± 1.8 38.8

In the case of mixing 1: 1 of wax and triplicate on the basis of wax, tyrosinase inhibitory activity was increased by 38.2%, and when tyrosine and reed roots were mixed 1: 1, tyrosinase inhibitory activity was increased by 38.8% .

[ Example  3] L- DOPA  Measurement of inhibitory activity

In Example 3, the L-DOPA inhibitory activity of each of the wax, triplex and reed roots was measured. 10 μL of the sample solution and mushroom tyrosinase (500 units / mL, Sigma Co., St. Louis, Mo., USA) were added to 210 μL of 0.1 M potassium phophate buffer (pH 7.0) Solution, and 60 μL of 3 mM L-DOPA (L-3,4-dihydroxyphenylalanine) solution was added thereto, followed by reaction at 37 for 5 minutes. After the reaction, the absorbance at 450 nm was measured.

L-DOPA inhibition rate (%) = [{(A-B) - (C-D)} / (A-B)] 100

A: The value of absorbance at 450 nm in the absence of L-DOPA and no sample added

B: Absorbance value at 450 nm in the absence of the sample and L-DOPA

C: value of absorbance at 450 nm for samples and L-DOPA addition

D: value of absorbance at 450 nm in the absence of L-DOPA, addition of sample

Fig. 3 and Table 3 show the results of L-DOPA inhibition of wax, triplex and reed roots, respectively.

sample L- DOPA  The inhibition (inhibition rate at 0.33 mg / ml, % ) The 4.0 ± 1.4 Triptych 4.7 ± 0.5 Reed roots 7.2 ± 0.1

[ Example  4] L- DOPA  Synergistic evaluation of inhibitory activity

In Example 4, the synergy of the L-DOPA inhibitory activity of wisteria, triplet, wisteria and reed root was evaluated on the basis of wax. In the same manner as in Example 3, L-DOPA inhibitory activity was measured by mixing samples in a ratio of 1: 1 (0.33 mg / ml) and 1: 1 ratio (0.33 mg / ml) Respectively. The synergy effect was calculated by the following formula.

Synergistic efficacy increase rate (%) = {(inhibition rate% - predicted inhibition rate%) / predicted inhibition rate%} × 100

The expected inhibition rate was calculated as follows:

Expected inhibition rate of A extract + B extract = ((Inhibition rate of A obtained in Example 1) + (Inhibition rate of B obtained in example 1)) / 2

4 and Table 4 show the synergistic effects on tyrosinase inhibitory activity and L-DOPA inhibitory activity of the wax, triplet, wilt and reed root, respectively.

sample Estimated inhibition rate ( % ) Inhibition rate % ) Synergistic Efficacy Growth Rate ( % ) The + Triptych (1: 1) 4.3 8.3 91.0 The + Reed roots (1: 1) 5.6 11.9 > 100

When L-DOPA inhibitory activity was increased by 91.0% when 1: 1 mixture of wheat and triplex on the basis of wheat, L-DOPA inhibitory activity increased by more than 100% when 1: 1 mixture of wheat and reed roots Can be confirmed.

Claims (12)

delete delete delete delete delete delete A whisker extract and a reed root extract in a weight ratio of 1: 1. delete delete delete A cosmetic composition comprising the skin whitening composition according to claim 7. A food composition comprising the skin whitening composition according to claim 7.

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