KR102429381B1 - 분석물을 막횡단 세공에 전달하는 방법 - Google Patents

분석물을 막횡단 세공에 전달하는 방법 Download PDF

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KR102429381B1
KR102429381B1 KR1020177013135A KR20177013135A KR102429381B1 KR 102429381 B1 KR102429381 B1 KR 102429381B1 KR 1020177013135 A KR1020177013135 A KR 1020177013135A KR 20177013135 A KR20177013135 A KR 20177013135A KR 102429381 B1 KR102429381 B1 KR 102429381B1
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클리브 브라운
다니엘 라이언 제럴드
앤디 헤론
다니엘 존 터너
제임스 화이트
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옥스포드 나노포어 테크놀로지즈 피엘씨
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    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
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    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

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KR1020177013135A 2014-10-17 2015-10-06 분석물을 막횡단 세공에 전달하는 방법 Active KR102429381B1 (ko)

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GBGB1418469.1A GB201418469D0 (en) 2014-10-17 2014-10-17 Method
GB1418469.1 2014-10-17
PCT/GB2015/052919 WO2016059375A1 (en) 2014-10-17 2015-10-06 Methods for delivering an analyte to transmembrane pores

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KR102429381B1 true KR102429381B1 (ko) 2022-08-03

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US (3) US10760114B2 (https=)
EP (1) EP3207157B2 (https=)
JP (1) JP6721581B2 (https=)
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CN (2) CN107002151B (https=)
GB (1) GB201418469D0 (https=)
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Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4737389A2 (en) 2011-05-27 2026-05-06 Oxford Nanopore Technologies PLC Coupling method
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
WO2015150786A1 (en) 2014-04-04 2015-10-08 Oxford Nanopore Technologies Limited Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
GB201418469D0 (en) * 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method
CA2976043A1 (en) 2015-02-10 2016-10-06 Multerra Bio, Inc. Apparatuses and methods for detecting molecules and binding energy
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
EP3464622B1 (en) * 2016-05-31 2021-04-28 F. Hoffmann-La Roche AG Methods and systems for nucleic acid sequencing by tunneling recognition
US10444179B2 (en) 2016-08-10 2019-10-15 Multerra Bio, Inc. Apparatuses and methods for detecting molecules and binding energy
US9816988B1 (en) * 2016-08-10 2017-11-14 Multerra Bio, Inc. Apparatuses and methods for detecting molecules and binding energy
GB201616590D0 (en) * 2016-09-29 2016-11-16 Oxford Nanopore Technologies Limited Method
GB201620450D0 (en) 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
GB201707140D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
GB2569977A (en) * 2018-01-05 2019-07-10 Oxford Nanopore Tech Ltd Method
GB201801768D0 (en) * 2018-02-02 2018-03-21 Oxford Nanopore Tech Ltd Synthesis method
US11980849B2 (en) 2018-02-09 2024-05-14 Ohio State Innovation Foundation Bacteriophage-derived nanopore sensors
GB201808554D0 (en) 2018-05-24 2018-07-11 Oxford Nanopore Tech Ltd Method
GB201809323D0 (en) 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method
WO2020084705A1 (ja) * 2018-10-24 2020-04-30 株式会社日立ハイテク 生体ポリマ分析デバイス及びそれを用いた分析装置、並びに分析方法
CN113166703A (zh) * 2018-11-21 2021-07-23 深圳华大生命科学研究院 核酸分子检测方法、检测装置和检测系统
US11624090B2 (en) 2019-01-16 2023-04-11 Armonica Technologies, Inc. Manipulating the translation of DNA strands across and through nanopore sequencing systems using Raman signatures to identify DNA bases and methods
GB201907244D0 (en) * 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
JP7590000B2 (ja) * 2019-05-28 2024-11-26 国立大学法人 東京大学 ポアデバイスを用いた分析装置および分析法
US11926819B2 (en) 2019-05-28 2024-03-12 The Regents Of The University Of California Methods of adding polymers to ribonucleic acids
GB201913997D0 (en) * 2019-09-27 2019-11-13 Oxford Nanopore Tech Ltd Method
WO2021113412A1 (en) * 2019-12-02 2021-06-10 The Regents Of The University Of California Lipid-DNA Labeling of Lipid Bilayer Particles for Amplification Quantitation
GB201917742D0 (en) * 2019-12-04 2020-01-15 Oxford Nanopore Tech Ltd Method
WO2022165276A1 (en) 2021-01-29 2022-08-04 Armonica Technologies, Inc. Enhancement structures for surface-enhanced raman scattering
CN113999291B (zh) * 2021-12-28 2022-04-15 北京齐碳科技有限公司 嵌入接头、锚定分子、分子膜、装置、方法及应用
GB202307494D0 (en) 2023-05-18 2023-07-05 Oxford Nanopore Tech Ltd Nanopore sequencing and preparation modular instrument

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012516146A (ja) * 2009-01-30 2012-07-19 オックスフォード ナノポア テクノロジーズ リミテッド 膜貫通配列決定における核酸構築物のためのアダプター
US20140134618A1 (en) * 2012-11-09 2014-05-15 Stratos Genomics, Inc. Concentrating a target molecule for sensing by a nanopore

Family Cites Families (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5198543A (en) 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
US5712126A (en) 1995-08-01 1998-01-27 Yale University Analysis of gene expression by display of 3-end restriction fragments of CDNA
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
US7527787B2 (en) 2005-10-19 2009-05-05 Ibc Pharmaceuticals, Inc. Multivalent immunoglobulin-based bioactive assemblies
EP2383776B1 (en) 1999-06-22 2015-02-25 President and Fellows of Harvard College Solid state nanopore device for evaluating biopolymers
US6849404B2 (en) 2001-05-07 2005-02-01 Bioneer Corporation Polymerase chain reaction of DNA of which base sequence is completely unidentified
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
ATE529734T1 (de) 2005-04-06 2011-11-15 Harvard College Molekulare charakterisierung mit kohlenstoff- nanoröhrchen-steuerung
KR100691806B1 (ko) * 2005-08-04 2007-03-12 삼성전자주식회사 비드 및 나노포어를 이용한 핵산 검출방법 및 검출장치
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
US8003319B2 (en) 2007-02-02 2011-08-23 International Business Machines Corporation Systems and methods for controlling position of charged polymer inside nanopore
US20110121840A1 (en) 2007-02-20 2011-05-26 Gurdial Singh Sanghera Lipid Bilayer Sensor System
EP2156179B1 (en) 2007-04-04 2021-08-18 The Regents of The University of California Methods for using a nanopore
WO2008124706A2 (en) 2007-04-06 2008-10-16 Arizona Board Of Regents Acting For And On Behalf Of Arizona State University Devices and methods for target molecule characterization
WO2009020682A2 (en) 2007-05-08 2009-02-12 The Trustees Of Boston University Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof
EP2014761B1 (en) 2007-06-22 2016-09-28 Sony Deutschland GmbH A device for processing an analyte and a method of processing and/or detecting an analyte using said device
WO2009035647A1 (en) 2007-09-12 2009-03-19 President And Fellows Of Harvard College High-resolution molecular graphene sensor comprising an aperture in the graphene layer
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
US8628940B2 (en) 2008-09-24 2014-01-14 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
WO2009151788A2 (en) * 2008-04-22 2009-12-17 The Washington University Universal anchor peptide for nanoparticles
WO2009132124A2 (en) 2008-04-24 2009-10-29 The Trustees Of Columbia University In The City Of New York Geometric patterns and lipid bilayers for dna molecule organization and uses thereof
EP2310534B1 (en) 2008-07-07 2018-09-05 Oxford Nanopore Technologies Limited Base-detecting pore
US20110229877A1 (en) 2008-07-07 2011-09-22 Oxford Nanopore Technologies Limited Enzyme-pore constructs
ATE544063T1 (de) 2008-07-17 2012-02-15 Koninkl Philips Electronics Nv Nanoporenvorrichtung und verfahren zur nukleinsäureanalyse
CA2774710C (en) * 2008-09-22 2016-08-02 University Of Washington Msp nanopores and related methods
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
EP2391726B1 (en) 2009-01-29 2015-09-23 Stratos Genomics Inc. High throughput nucleic acid sequencing by expansion and related methods
KR20110125226A (ko) 2009-01-30 2011-11-18 옥스포드 나노포어 테크놀로지즈 리미티드 혼성화 링커
US8986928B2 (en) 2009-04-10 2015-03-24 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods
CN102405410B (zh) 2009-04-20 2014-06-25 牛津楠路珀尔科技有限公司 脂质双层传感器阵列
US9127313B2 (en) 2009-12-01 2015-09-08 Oxford Nanopore Technologies Limited Biochemical analysis instrument
CN102834716B (zh) * 2010-02-23 2016-03-30 华盛顿大学 人工分枝菌酸膜
JP5764296B2 (ja) 2010-03-31 2015-08-19 株式会社日立ハイテクノロジーズ 生体ポリマーの特性解析法
WO2012033524A2 (en) 2010-09-07 2012-03-15 The Regents Of The University Of California Control of dna movement in a nanopore at one nucleotide precision by a processive enzyme
CN103502448B (zh) 2010-11-12 2017-03-29 Gen9股份有限公司 核酸合成的方法和设备
KR101939420B1 (ko) 2011-02-11 2019-01-16 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 세공
US9347929B2 (en) † 2011-03-01 2016-05-24 The Regents Of The University Of Michigan Controlling translocation through nanopores with fluid wall
EP2699903B1 (en) 2011-04-20 2018-07-18 Life Technologies Corporation Methods, compositions and systems for sample deposition
EP4737389A2 (en) 2011-05-27 2026-05-06 Oxford Nanopore Technologies PLC Coupling method
JP6298404B2 (ja) * 2011-07-25 2018-03-20 オックスフォード ナノポール テクノロジーズ リミテッド 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法
EP2573554A1 (en) 2011-09-21 2013-03-27 Nxp B.V. Apparatus and method for bead detection
JP6457811B2 (ja) 2011-09-23 2019-01-23 オックスフォード ナノポール テクノロジーズ リミテッド ポリマー単位を含むポリマーの解析
CN104039979B (zh) 2011-10-21 2016-08-24 牛津纳米孔技术公司 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法
US9267917B2 (en) 2011-11-04 2016-02-23 Pacific Biosciences Of California, Inc. Nanopores in zero mode waveguides
US9617591B2 (en) 2011-12-29 2017-04-11 Oxford Nanopore Technologies Ltd. Method for characterising a polynucleotide by using a XPD helicase
AU2012360244B2 (en) 2011-12-29 2018-08-23 Oxford Nanopore Technologies Limited Enzyme method
WO2013119784A1 (en) * 2012-02-08 2013-08-15 Brown University Methods of sequencing nucleic acids using nanopores and active kinetic proofreading
KR102083695B1 (ko) 2012-04-10 2020-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 리세닌 기공
JP6429773B2 (ja) 2012-07-19 2018-11-28 オックスフォード ナノポール テクノロジーズ リミテッド 酵素構築物
CA2879261C (en) 2012-07-19 2022-12-06 Oxford Nanopore Technologies Limited Modified helicases
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
US9551023B2 (en) * 2012-09-14 2017-01-24 Oxford Nanopore Technologies Ltd. Sample preparation method
JP6375301B2 (ja) 2012-10-26 2018-08-15 オックスフォード ナノポール テクノロジーズ リミテッド 液滴界面
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
RU2015139609A (ru) 2013-02-20 2017-03-24 Ив Байомедикал, Инк. Способы и композиции для секвенирования нуклеиновой кислоты, основанные на наноструктуре
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
CA2901545C (en) 2013-03-08 2019-10-08 Oxford Nanopore Technologies Limited Use of spacer elements in a nucleic acid to control movement of a helicase
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
CN117947149A (zh) 2013-10-18 2024-04-30 牛津纳米孔科技公开有限公司 经修饰的酶
JP6461943B2 (ja) * 2013-10-23 2019-01-30 ジェニア・テクノロジーズ・インコーポレイテッド ナノポアを備えた高速分子検知
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
WO2015150786A1 (en) 2014-04-04 2015-10-08 Oxford Nanopore Technologies Limited Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
GB201418469D0 (en) * 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012516146A (ja) * 2009-01-30 2012-07-19 オックスフォード ナノポア テクノロジーズ リミテッド 膜貫通配列決定における核酸構築物のためのアダプター
US20140134618A1 (en) * 2012-11-09 2014-05-15 Stratos Genomics, Inc. Concentrating a target molecule for sensing by a nanopore

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