KR102428010B1 - Skin external composition - Google Patents

Abstract

The present invention relates to a composition for external application for skin comprising a Tamarix chinensis extract as an active ingredient. One embodiment provides the composition for external application for skin, which is excellent in maintaining scalp barrier functions and suppressing inflammation of the scalp while being safe and having few side effects on human body.

Description

Composition for external skin application {SKIN EXTERNAL COMPOSITION}

It relates to a composition for external application for skin.

The dermal sebaceous glands are glands located in the dermis, adjacent to the hair follicles, and are complete glands that secrete a mixture of lipids known as sebum. It is present all over the body except for the palms and soles of the feet, and is most commonly distributed on the face and scalp. The term 'sebaceous' refers to secretions of the sebaceous glands containing triglycerides, free fatty acids, wax esters, scalene, cholesterol and cholesteryl esters.

The skin is composed of the epidermis, dermis, and subcutis, and the stratum corneum of the epidermis provides an environment in which the skin can maintain normal biological functions by suppressing the loss of moisture and electrolytes through the skin. When external harmful factors such as toxic substances, microorganisms, mechanical stimuli, and UV rays invade through the skin, it serves as the primary defense line. In particular, keratinocyte intercellular lipids in the stratum corneum are known to play an important role in performing skin barrier functions, such as suppressing excessive loss of body fluids and electrolytes through the skin and controlling absorption and permeation of foreign substances.

Sebum produced excessively in the sebaceous glands can cause skin diseases such as making the skin oily and causing acne and seborrheic dermatitis. Sebum is produced by sebaceous gland cells and secreted to the skin surface through the hair follicle duct. It is known that excessively generated sebum changes the structure of lipid between keratinocytes on the skin surface, thereby lowering the skin barrier function. In addition, it is known that overgrowth of bacteria causing scalp inflammation including seborrheic dermatitis causes scalp inflammation including seborrheic dermatitis. Therefore, it is considered an important task to suppress excessive production of sebum and inflammation in the scalp.

One embodiment provides a composition for external application for skin that is safe and has fewer side effects to the human body and is excellent in maintaining the scalp barrier function and inhibiting inflammation of the scalp.

One embodiment provides a composition for external application for skin comprising a tamarisk extract as an active ingredient.

The composition for external application for skin may be for improving the condition of the scalp.

The tamarisk extract may be extracted from leaves, flowers, fruits, stems, roots, seeds, or combinations thereof.

The concentration of the tamarisk extract may be 1 ppm to 1000 ppm.

The tamarisk extract may be included in an amount of 0.001% to 10% by weight based on the total amount of the composition for external application for skin.

The tamarisk extract can suppress excessive production of sebum caused by bacteria that cause scalp inflammation.

The tamarisk extract can inhibit the expression of inflammatory cytokines by bacteria causing scalp inflammation.

The tamarisk extract may inhibit lipase activity of the causative agent of scalp inflammation.

The causative agent of scalp inflammation may include Malassezia restricta, Malassezia globosa, Cutibacterium acnes, or a combination thereof.

The tamarisk extract may be extracted using water, an organic solvent, or a mixture thereof.

The tamarisk extract may be extracted using the organic solvent, and the organic solvent may include an alcohol having 1 to 10 carbon atoms.

The composition for external application for skin includes solutions, suspensions, emulsions, pastes, lotions, powders, soaps, surfactant-containing cleansing oils, powder foundations, emulsion foundations, wax foundations, shampoos, rinses, tonics, sprays, or combinations thereof. It may be in the form

The composition for external application for skin may be a cosmetic composition for improving scalp condition, a quasi-drug composition for improving scalp condition, or a pharmaceutical composition for improving scalp condition.

The composition for external application for skin according to one embodiment has few side effects and is safe to the human body, and has excellent effects of maintaining the scalp barrier function and inhibiting inflammation of the scalp, thereby exhibiting an excellent effect of improving the scalp condition.

Hereinafter, the embodiments will be described in detail so that those of ordinary skill in the art can easily implement them. However, the embodiments may be embodied in many different forms and are not limited to the embodiments described herein.

The composition for external application for skin according to an embodiment may include a tamarisk extract as an active ingredient.

The tamarisk ( Tamarix chinensis ) used to obtain the tamarisk extract is a deciduous small arboreous tree of the dicotyledonous Violet family, mainly grown in the south of central Korea. The height is 5m, the flowers bloom twice a year, the fruits are capsules (), ripen in October, and the seeds have hairs. Originating from China, it is planted as an ornamental in gardens, and the leaves are used for medicinal purposes.

The composition for external application for skin according to an embodiment may be for improving the condition of the scalp. When the composition for external application for skin containing the tamarisk extract as an active ingredient is used on the scalp, excessive production of sebum induced by bacteria causing scalp inflammation and the expression of inflammatory cytokines are suppressed, and the activity of lipase By inhibiting the scalp barrier function and the effect of inhibiting inflammation of the scalp is improved, an excellent effect of improving the scalp condition can be obtained.

The tamarisk extract may be extracted from leaves, flowers, fruits, stems, roots, seeds, or a combination thereof, and may be extracted using, for example, whole plants.

The concentration of the tamarisk extract may be 1 ppm to 1000 ppm, for example, 1 ppm to 900 ppm, 1 ppm to 800 ppm, and 5 ppm to 700 ppm . When the concentration of the tamarisk extract is within the above range, the excessive production of sebum and the expression of inflammatory cytokines are suppressed, and the activity of lipase is inhibited, so that the scalp barrier function and the effect of inhibiting inflammation of the scalp can be improved.

The tamarisk extract may be included in an amount of 0.001 wt% to 10 wt% based on the total amount of the composition for external application for skin, for example, 0.01 wt% to 10 wt%, 0.1 wt% to 10 wt%, 0.1 wt% to 5 wt% It may be included in weight percent . When the tamarisk extract is included within the above content range, the stability of the formulation is improved, and the effect of maintaining the scalp barrier function and inhibiting inflammation of the scalp is excellent, thereby exhibiting an excellent effect of improving the condition of the scalp. It can be usefully used as a pharmaceutical composition.

The tamarisk extract can suppress excessive production of sebum caused by bacteria that cause scalp inflammation. That is, when human sebocytes are treated with the culture solution of the bacteria causing scalp inflammation and the tamarisk extract according to one embodiment, it is possible to effectively inhibit the production of sebum induced by the bacteria causing scalp inflammation.

The tamarisk extract can suppress the expression of inflammatory cytokines caused by bacteria causing scalp inflammation. That is, when the human sebaceous gland cells (Human Sebocyte) are treated with the culture medium of the bacteria causing scalp inflammation and the tamarisk extract according to one embodiment, the inflammatory cytokines IL-1β (interleukin-1β), IL-6 ( Interleukin-6), IL-8 (interleukin-8) and TNF-α (tumor necrosis factor-α) can be effectively inhibited.

The tamarisk extract can inhibit the activity of lipase by the causative agent of scalp inflammation. That is, when the tamarisk extract according to an embodiment is treated in the culture medium of the bacteria causing scalp inflammation, the activity of lipase secreted by the bacteria causing scalp inflammation can be effectively inhibited.

The scalp inflammation causative agent may include Malassezia restricta , Malassezia globosa , Cutibacterium acnes , or a combination thereof. The Malassezia restricta corresponds to a bacterium that induces excessive production of sebum in human sebaceous gland cells. The Malassezia globosa secretes lipase. Lipase decomposes triglyceride and ester, which are components of sebum, to produce linoleic acid and oleic acid, which are unsaturated fatty acids that are irritating to the skin. The Cutibacterium acnes induces the expression of inflammatory cytokines IL-1β, IL-6, IL-8 and TNF-α. In addition, by secreting lipase, linoleic acid and oleic acid are produced, which are unsaturated fatty acids that are irritating to the skin.

The tamarisk extract may be extracted using water, an organic solvent, or a mixture thereof. Methods for extracting the tamarisk extract include hot water extraction, cold extraction, solvent extraction, steam distillation, elution, compression, ultrasonic extraction, filtration, reflux extraction, and the like, either alone or in combination of two or more methods. can do. Among the above methods, for example, the tamarisk extract may be extracted by a solvent extraction method. It can be obtained by a solvent extraction method in which an extraction solvent is added to the tamarisk outpost, cooled for a certain period of time, filtered and concentrated under reduced pressure to recover the extract.

The amount of the extraction solvent may be 20 ml to 80 ml, for example, 30 ml to 70 ml per 1 g of dry weight of the tamarisk outpost . and Chilling may be carried out at room temperature for 1 to 5 days, or, for example, at 15° C. to 25° C. for 1 to 3 days. When extracted within the above temperature range, it is effective in preventing decay, discoloration and odor, and when extracted within the above time range, extraction is performed well to secure sufficient active ingredients.

The solvent extraction method is to extract using water, an organic solvent, or a mixture thereof, for example, an organic solvent may be used.

The organic solvent may include an alcohol having 1 to 10 carbon atoms, for example, methanol, ethanol, propyl alcohol, butyl alcohol, glycerin, propylene glycol, butylene glycol, amyl alcohol, n-hexyl alcohol, n-heptane It may include one or more selected from the group consisting of ol and n-octanol, and for example, ethanol may be included.

The composition for external application for skin according to an embodiment is a solution, suspension, emulsion, paste, lotion, powder, soap, surfactant-containing cleansing oil, powder foundation, emulsion foundation, wax foundation, shampoo, rinse, tonic, spray, or any of these It may have a formulation comprising a combination, but is not limited thereto.

The composition for external application for skin according to an embodiment may further include additives according to uses in addition to the tamarisk extract. The additives may be used alone or two or more types of moisturizers, thickeners, surfactants, oil agents, preservatives, antioxidants, alcohols, fragrances, pH adjusters, natural extracts, and the like.

The composition for external application for skin according to an embodiment may be a cosmetic composition for improving scalp condition, a quasi-drug composition for improving scalp condition, or a pharmaceutical composition for improving scalp condition.

The formulation of the cosmetic composition for improving scalp condition is not particularly limited, and may be appropriately selected according to the purpose. For example, flexible lotion, astringent lotion, nourishing lotion, massage cream, essence, nourishing cream, pack, powder, waxy cream, etc. may be prepared in the formulation, but is not limited thereto.

The quasi-drug composition for improving scalp condition may be in the form of shampoo, rinse, treatment, hair pack, spray, etc., but is not limited thereto.

The pharmaceutical composition for improving scalp condition may be a transdermal dosage form such as a lotion, ointment, gel, cream, patch, etc., but is not limited thereto.

The pharmaceutical composition can be used for diseases related to the scalp barrier, for example, it can be used for diseases such as atopic dermatitis, seborrheic dermatitis, acne, psoriasis, alopecia areata, hair loss on the scalp.

Hereinafter, specific embodiments of the present invention are presented. However, the examples described below are only for specifically illustrating or explaining the present invention, and the present invention is not limited thereto. In addition, since the contents not described herein can be technically inferred sufficiently by those skilled in the art, the description thereof will be omitted.

Preparation Example 1: Preparation of tamarisk extract

100 g of tamarisk (obtaining place: Jeollabuk-do) whole grass was collected, put in 5 L of 70% aqueous ethanol solution, cooled at room temperature (15 ° C. ~ 25 ° C) for 48 hours, and filtered with Whatman #5 filter paper. The filtered extract was concentrated under reduced pressure at 45° C. or lower to obtain 15 g of the extract. In order to proceed with the cell experiment, the extract was dissolved in DMSO (dimethyl sulfoxide) to prepare a sample.

Culture conditions of human sebocytes and normal human epidermal keratinocytes (NHEK) - Cell culture conditions

Human sebocytes were purchased from Celprogen (USA) and used. The culture medium was cultured at 37° C. and 5% CO ₂ condition using human sebocyte complete media with serum (M36079-01S, Celprogen, USA).

Normal human epidermal keratinocytes (NHEK) were purchased from the American type culture collection (ATCC, USA) and used. The culture medium was cultured at 37° C. and 5% CO ₂ conditions using a dermal cell basal medium (ATCC, USA) to which a keratinocyte growth kit (ATCC, USA) was added.

Culture conditions of Malassezia restricta (M.restricta), Malassezia globosa (M.globosa) and Cutibacterium acnes (C.acnes) - strain culture conditions

Malassezia restricta (M.restricta, KCTC 27527), Malassezia globosa (Malassezia globosa, M.globosa, KCTC 27535), and Cutibacterium acnes, C .acnes, KCTC 3314) are strains that cause scalp inflammation, and were distributed from the Korean Collection for Type Cultures (KCTC) of the Korea Research Institute of Bioscience and Biotechnology. Malassezia restricta and Malassezia globosa were cultured at 32°C using leeming and notman agar (LNA, Kisanbio, Korea) medium, and subcultured once every 3 days. Cutibacterium acnes was cultured at 37°C using brain heart infusion (BHI) medium, and subcultured once every 2 days.

Experimental Example 1: Malassezia restricta ( Malassezia restricta, M.restricta) Evaluation of inhibition of excessive sebum production induced by

In order to evaluate the inhibition of excessive inflammatory sebum production by the tamarisk extract according to Preparation Example 1, a human sebocyte culture medium was added to a 24-well plate at a concentration of 1X10 ⁵ cells/well. After dispensing, the cells were cultured for 24 hours under the above cell culture conditions.

After removing the culture medium, the human sebocytes were transferred to a serum-free medium (human sebocyte serum free media, M36079-01, Celprogen, USA), and Malassezia restrick according to the culture conditions of the strain in a serum-free medium containing human sebaceous gland cells. Sebum production was induced by treating other cultures with 2.5% (v/v). Then, the tamarisk extract according to Preparation Example 1 was simultaneously treated at concentrations of 12.5 ppm and 25 ppm, respectively, to measure the sebum production inhibitory effect. Oil Red O (Oil Red O) staining method was used to measure the sebum production inhibitory effect, and more specifically, The following process was performed. The Malassezia restricta culture medium and the tamarisk extract were treated at the same time, and then left for 48 hours. Then, the human sebaceous gland cells were washed with PBS (phosphate buffer saline), and the washed human sebaceous gland cells were fixed with 10% formaldehyde solution for 30 minutes. The fixed human sebaceous gland cells were washed twice with PBS and once with 50% isopropanol, and then stained with 0.4% Oil Red O solution for 45 minutes. The stained human sebaceous gland cells were washed once with 70% ethanol and twice with PBS. After washing, the well plate was sufficiently dried, and the Oil Red O solution stained with human sebaceous gland cells was dissolved again with 100% isopropanol. Then, the absorbance was measured at a wavelength of 520 nm with a micro plate reader to measure the amount of triglycerides produced in the cells. At this time, a group in which human sebaceous gland cells were treated with a culture medium of Malassezia restraint and not treated with a tamarisk extract was treated as a control group, and a group in which human sebaceous gland cells were not treated with both a culture medium of Malassezia restricta and a tamarisk extract was treated as a control group. It was used as an untreated control, and the results are shown in Table 1 below.

sample Sebum production rate (%) Inhibition rate of increase in sebum production
(%, compared to control (M)) untreated control 100 - Control (M.restricta culture solution 2.5%) (M) 142.02 0 M + satellite extract 12.5 ppm 116.26 18.14 25 ppm of M+Satellite Extract 111.96 21.17

Through Table 1, the control group treated only with the Malassezia restricta culture medium on human sebaceous gland cells significantly induced sebum production compared to the untreated control group, and the human sebaceous gland cells were treated with the Malassezia restricta culture medium and in one embodiment. It was confirmed that when the tamarisk extract was treated, it effectively suppressed the production of sebum induced by the Malassezia restricta culture medium.

Experimental Example 2: Cutibacterium acnes ( Cutibacterium acnes, C. acnes ) Evaluation of inhibition of inflammatory cytokine expression in human sebaceous gland cells induced by

For evaluation of inhibition of inflammatory cytokine expression by the tamarisk extract according to Preparation Example 1, human sebocytes were dispensed in a 6-well plate at a concentration of 1X10 ⁶ cells/well, and then the cells The culture conditions were incubated for 24 hours. After removing the culture medium, human sebaceous gland cells were transferred to a serum-free medium, and an inflammatory response was induced by treating a serum-free medium with human sebaceous gland cells with 10% (v/v) of Cutibacterium acnes according to the culture conditions of the strain. . Then, the tamarisk extract according to Preparation Example 1 was simultaneously treated at a concentration of 25 ppm for 24 hours, and then intracellular inflammatory cytokines IL-1β (interleukin-1β), IL-6 (interleukin-6), IL-8 (interleukin-8) and TNF-α (tumor necrosis factor-α) gene expression levels were confirmed through quantitative real time PCR (polymerase chain reaction) (quantitative real time-PCR, qRT-PCR) experiments. The specific process is as follows.

RNA was extracted from the cells using an easy-spin total RNA extraction kit (iNtRON BIOTECHNOLOGY, Korea). After taking a certain amount of RNA, cDNA was synthesized using Maxime ^TM RT Premix Oligo dt (iNtRON BIOTECHNOLOGY, Korea). To measure gene expression, the ⁷⁵⁰⁰ Fast Real-time PC system (7500 Fast Real -Time PCR System, Applied Biosystems, USA) was reacted in the instrument. The PCR process was performed for 40 cycles, and the results were analyzed and quantified using Excel, and a cycle of threshold (CT) (comparative CT) method was used as the analysis method. At this time, a group in which human sebaceous gland cells were treated with a culture solution of Cutibacterium acnes and not treated with a tamarisk extract was treated as a control group, and a group in which human sebaceous gland cells were not treated with both a culture solution of Cutibacterium acnes and a tamarisk extract was not treated. It was used as a control, and the results are shown in Table 2 below.

sample IL-1β Ratio IL-6 Ratio IL-8 Ratio TNF-α ratio untreated control 1.0 1.0 1.0 1.0 Control ( C. acnes culture 10%) 1.95 6.61 1.35 29.00 C+ satellites 25 ppm 1.12 3.16 1.09 7.60

- Ratio: Corresponds to the inflammatory cytokine gene expression ratio compared to the untreated control group.

From Table 2, it was confirmed that the tamarisk extract according to one embodiment effectively reduced the gene expression of the inflammatory cytokines IL-1β, IL-6, IL-8 and TNF-α induced by Cutibacterium acnes. did.

The control group in which only the human sebaceous gland cells were treated with the culture medium of Cutibacterium acnes significantly induced the expression of inflammatory cytokines compared to the control group without treatment, and the culture medium of Cutibacterium acnes and the tamarisk extract according to one embodiment were applied to the human sebaceous gland cells. When treated, it was confirmed that the expression of inflammatory cytokines induced by the culture medium of Cutibacterium acnes was effectively suppressed.

Experimental Example 3: Malassezia globosa ( Malassezia globosa, M. globosa) and Cutibacterium acnes ( Cutibacterium acnes, C. acnes ) lipase activity inhibition evaluation

Lipase secreted by Malassezia globosa and Cutibacterium acnes breaks down triglyceride and ester, which are components of sebum, to produce unsaturated fatty acids, linoleic acid and oleic acid, which are irritating to the skin. acid) is formed. Of the tamarisk extract according to Preparation Example 1, Malassezia Globosa In order to evaluate the inhibitory effect of lipase secreted by Cutibacterium acnes, a lipase assay was performed by measuring lipolytic enzyme activity, and specifically, the following procedure was performed.

In order to evaluate the effect of inhibiting lipase activity by the tamarisk extract according to Preparation Example 1, first, 30 μl of 90 mM citrate buffer, 10 mM 4-Nite in 200 μl of Malassezia globosa culture according to the strain culture conditions. 30 μl of lophenyl dodecanoate (p-NPD), 30 μl of 5% triton X-100, and 610 μl of Tris-HCl buffer (pH 8.0) were added. An enzyme buffer was prepared. 100 μl of the tamarisk extract according to Preparation Example 1 at a concentration of 500 ppm was added to the enzyme buffer and reacted at 30° C. for 1 hour. At this time, Malassezia globosa A group in which the culture medium was not treated with the tamarisk extract was used as a control group. And the degree of hydrolysis of 4-nitrophenyl dodecanoate (p-NPD) to 4-nitrophenol (p-nitrophenol) was measured by absorbance at a wavelength of 405 nm. The effect of inhibiting the lipase activity of Cutibacterium acnes was also confirmed in the same way as above, and a group in which the tamarisk extract was not treated to Cutibacterium acnes was used as a control group. The lipase activity rate was expressed as a relative value of the lipase activity when the tamarisk extract was treated with the lipase activity of the control group not treated with the tamarisk extract being 100. The lipase activity rate was calculated using the following formula (1).

Formula (1) Lipase activity rate (%) = (B / A) X 100

- A: Absorbance of the control group not treated with the tamarisk extract sample

- B: Absorbance of the experimental group treated with the tamarisk extract sample

The measurement results are shown in Table 3 below.

sample M.globosa secretion
Inhibition of lipase activity C.acnes secretion
Inhibition of lipase activity A B lipase
Activity rate (%) A B lipase
Activity rate (%) tamarisk extract
500 ppm 2.29 1.39 60.63 2.17 2.01 92.44

As a result, as shown in Table 3, it was confirmed that the tamarisk extract effectively inhibited the activity of lipase secreted by Malassezia globosa and Cutibacterium acnes. That is, it was found that the tamarisk extract according to one embodiment is effective in inhibiting the production of unsaturated fatty acids that cause skin irritation and cause scalp inflammation.

Experimental Example 4: Safety test for skin

In order to confirm the skin safety of the composition for external application for skin containing the tamarisk extract according to one embodiment, a patch test applied with the tamarisk extract was conducted on 30 adult males and females to evaluate the skin safety of the composition of the present invention. . A specific measurement method is as follows.

After attaching the patch coated with the tamarisk extract to the skin, the patch was removed after 24 hours. The first reading was performed 1 hour after the patch was removed, and the second reading was performed 24 hours after the patch was removed. In order to determine the skin irritation intensity of the tamarisk extract sample, weights were given according to the degree of positive skin reaction, and the average skin reaction score of the skin was obtained, and the skin irritation of the extract sample was visually determined, and the results are shown in Table 4 below. .

Sample name
1 hour later


after 24 hours
average reaction score of the skin Judgment tamarisk extract
1000 ppm Skin positivity weight 0.5 One 2 3 0.5 One 2 3
0
non-irritant Number of people (persons) 0 0 0 0 0 0 0 0

As shown in Table 4 above, no one showed a positive skin reaction corresponding to a weight of 0.5 to 3 at the first and second readings after removing the patch coated with 1000 ppm of the tamarisk extract, and through this, It was confirmed that there was no irritation to the skin when the ryu extract was applied to the skin. That is, it was found that there was no irritation to the skin in the case of the composition for external application for skin including the tamarisk extract according to one embodiment.

Hereinafter, based on the results of the above-described experimental examples, various formulation examples of the external skin composition including the tamarisk extract are formulated and presented. However, these formulation examples are for describing one embodiment, and it is obvious to those of ordinary skill in the art that the formulation in one embodiment is not limited to these formulation examples.

Formulation Example 1: Rinse

Ingredient name Content (wt%) Tamarisk extract (Preparation Example 1) 1.0 cetyl alcohol 2 stearyl alcohol 2.5 behenyl alcohol 0.5 Spices 0.4 silicone emulsion 0.4 cyclomethicone 1.0 Dimethyldistearylammonium chloride 0.1 citric acid 0.1 - 1 Purified water remaining amount

Formulation Example 2: Ampoule

Ingredient name Content (wt%) Tamarisk extract (Preparation Example 1) 1.0 ethanol 10.0 propylene glycol 2.0 1,3-butylene glycol 1.0 cetyltrimethylammonium chloride 3 Dimethyldistearylammonium chloride 0.1 lauryl alcohol 3 myristyl alcohol 5 cetyl alcohol One Spices 0.5 dyes 0.0001 Purified water remaining amount

Formulation Example 3: Tonic

Ingredient name Content (wt%) Tamarisk extract (Preparation Example 1) 1.0 ethanol 40 propylene glycol 10 Polysorbate 20 5 Spices 0.5 dyes 0.0001 Purified water remaining amount

As shown in Tables 5 to 7, in the case of the composition for external application for skin composed of various formulations including the tamarisk extract according to one embodiment, it is safe with few side effects to the human body, and excessive production of sebum caused by bacteria causing scalp inflammation and inflammatory cytokines By inhibiting the expression of Cain and inhibiting the activity of lipase, it is possible to maintain the scalp barrier function and improve the effect of inhibiting inflammation of the scalp, that is, it can exhibit an excellent effect of improving the scalp condition.

Although preferred embodiments of the present invention have been described above, the present invention is not limited thereto, and it is possible to carry out various modifications within the scope of the claims, the detailed description of the invention, and the accompanying drawings. It goes without saying that it falls within the scope of the invention.

Claims (13)

containing tamarisk extract as an active ingredient,
The tamarisk extract inhibits scalp inflammation caused by bacteria causing scalp inflammation,
The scalp inflammation causative agent is Malassezia restricta ( Malassezia restricta ), Malassezia globosa ( Malassezia globosa ), Cutibacterium acnes ), or a combination thereof. composition. delete In claim 1,
wherein the tamarisk extract is extracted from leaves, flowers, fruits, stems, roots, seeds, or a combination thereof of tamarisk. In claim 1,
The concentration of the tamarisk extract is 1 ppm to 1000 ppm, the composition for external application for skin for improving scalp condition. In claim 1,
The tamarisk extract is included in an amount of 0.001% to 10% by weight based on the total amount of the composition for external application for skin. In claim 1,
The tamarisk extract inhibits excessive production of sebum by Malassezia restricta , which is a causative agent of scalp inflammation. In claim 1,
The tamarisk extract inhibits the expression of inflammatory cytokines by Cutibacterium acnes , a bacterium that causes scalp inflammation. In claim 1,
The tamarisk extract inhibits the lipase activity of Malassezia globosa and Cutibacterium acnes , which are bacteria that cause scalp inflammation. delete In claim 1,
The composition for external application for skin for improving scalp condition, wherein the tamarisk extract is extracted using water, an organic solvent, or a mixture thereof. In claim 10,
The tamarisk extract is extracted using the organic solvent,
The organic solvent is an external skin composition for improving scalp condition comprising alcohol having 1 to 10 carbon atoms. In claim 1,
The composition for external application for scalp condition improvement is a solution, suspension, emulsion, paste, lotion, powder, soap, surfactant-containing cleansing oil, powder foundation, emulsion foundation, wax foundation, shampoo, rinse, tonic, spray, or any of these A composition for external application for skin for improving scalp condition that has a formulation comprising a combination. In claim 1,
The composition for external application for scalp condition improvement is a cosmetic composition for improving scalp condition, a quasi-drug composition for improving scalp condition, or a pharmaceutical composition for preventing or treating atopic dermatitis, seborrheic dermatitis, acne or psoriasis occurring on the scalp. A composition for external application for skin.