KR102392547B1 - Preparation method for collagen powder with improved bioabsorbability and collagen powder prepared by the same - Google Patents

Abstract

The present invention relates to a method for producing a collagen powder with improved bioabsorbability and a collagen powder prepared therefrom, and the prepared collagen powder can be used for food or cosmetics.

Description

Preparation method for collagen powder with improved bioabsorbability and collagen powder prepared therefrom

The present invention relates to a method for producing a collagen powder with improved bioabsorbability and a collagen powder prepared therefrom, and the prepared collagen powder can be used for food or cosmetics.

In general, collagen is a material that fills the gaps between cells in an animal's body. It is a fibrous hard protein that is contained in the highest content in the body, and is a material made in multicellular animals rather than single cells.

In addition, collagen is a type of protein, has a long and thin fiber shape, and is a helical polymer protein composed of three polypeptide chains with a molecular weight of about 100,000. Collagen is the main component of the dermal layer and connective tissue of the skin and accounts for 70% of the skin, 90% of the protein constituting the bone, and 20% of the bone weight.

Collagen is an animal fibrous protein composed of about 18 amino acids such as proline, oxyproline, glycine, and glutamic acid. In the case of humans, it is a special structural protein that accounts for 30% of the 5,000 types of proteins that make up the human body. Collagen is known to be difficult to absorb due to its high molecular weight compared to most proteins, so it is known to make collagen peptides or low molecular weight collagen by decomposing collagen to a molecular weight of 5,000 Da and the like. Low molecular weight collagen is further degraded by proteolytic enzymes in the human body and finally absorbed into the human body in the form of amino acids.

Collagen administered by the oral route is absorbed by the peptide transporter (PEPT1) in the form of a low-molecular-weight peptide with between 2-5 amino acid residues and transferred into the blood. When applied by the transdermal route, it is known that the molecular weight that can be absorbed into the dermal layer containing fibroblasts synthesizing collagen is about 500 Da. Therefore, it would be desirable for a composition comprising orally and transdermally administered collagen to have a low molecular weight in order to substantially interact with fibroblasts in the body.

However, most of the previously commercialized collagen powders only mention improvement of bioabsorbability through lowering the molecular weight of collagen, and there has been no study on improvement of bioabsorbability through surface treatment of collagen powder.

Korean Patent No. 1218898 Korean Patent No. 1393403

H. A. E. Benson et al., Letters in Peptide Science, 10: 615-620, 2003

It is an object of the present invention to provide a method for producing collagen powder with improved bioabsorbability, comprising: treating the collagen powder with atmospheric pressure plasma for 1 to 5 minutes at a plasma intensity of 150 to 300 W.

Another object of the present invention is to provide a collagen powder with improved bioabsorbability prepared by the above method.

Another object of the present invention is to provide a food composition comprising the collagen powder with improved bioabsorption.

Another object of the present invention is to provide a cosmetic composition comprising collagen powder with improved bioabsorption.

The present invention provides a method for producing collagen powder with improved bioabsorbability, comprising: treating the collagen powder with an atmospheric pressure plasma for 1 to 5 minutes at a plasma intensity of 150 to 300 W.

Before the plasma treatment, adjusting the moisture content of the collagen powder to 8 to 20% by weight; may further include.

Adjusting the moisture content to 8 to 20 wt% may include adding an organic acid aqueous solution.

After the plasma treatment, drying the collagen powder with superheated steam; may include more.

The molecular weight of the collagen powder may be 300 to 5000 Da.

In addition, the present invention provides a collagen powder with improved bioabsorption prepared by the above method.

In addition, the present invention provides a food composition comprising the collagen powder with improved bioabsorption.

In addition, the present invention provides a cosmetic composition comprising collagen powder with improved bioabsorption.

The present invention relates to a method for producing a collagen powder with improved bioabsorbability and a collagen powder prepared therefrom, and the prepared collagen powder can be used for food or cosmetics.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a method for producing collagen powder with improved bioabsorbability, comprising: treating the collagen powder with an atmospheric pressure plasma for 1 to 5 minutes at a plasma intensity of 150 to 300 W.

The collagen is collagen extracted from raw materials obtained from terrestrial animals, plants, or marine animals. The terrestrial animals may be pigs, cattle, etc., and preferably, pig skin, cow skin, pork bone, and beef bone may be used. Carrots, red ginseng, pine needles, sesame seeds, enoki mushrooms, oyster mushrooms, etc. may be used as the plant, and the marine animal may be any one or more selected from the group consisting of fish, fish scales, fish skin, molluscs and crustaceans, preferably It is extracted from fish scales.

The collagen powder preferably includes a low molecular weight collagen powder prepared by ultrafiltration or gel filtration of the collagen raw material after acid treatment or enzyme treatment.

The acid treatment is to treat the collagen with a hydrochloric acid solution having a concentration of 0.003 to 3M. After the acid treatment, an alkali is added and neutralized by adding an alkali until the pH is about 7.0 to 7.5. It is preferable to use sodium hydroxide or calcium carbonate as the alkali.

The enzyme may be any one or more proteolytic enzymes selected from the group consisting of protamax, flavourzyme, alcalase, and neutrase.

In the case of the enzyme treatment, 0.1 to 1 part by weight of an enzyme is added to 100 parts by weight of collagen, and hydrolyzed collagen obtained by hydrolysis at 40 to 60° C. for 2 to 24 hours is subjected to ultrafiltration or gel filtration to obtain a specific molecular weight fraction. It may be manufactured according to the

After the enzyme treatment step is completed, the step of inactivating or sterilizing the enzyme may be further included. Specifically, the step of inactivating or sterilizing the enzyme may be performed by heating at 80° C. or higher for a predetermined time.

The average molecular weight of the low molecular weight collagen may be 300 to 5000 Da, preferably 300 to 1500 Da, more preferably 300 to 500 Da.

Plasma refers to a state in which the energy of a gas is parallel to each other as it is separated into ions and electrons when a gas has higher energy. It is a technology that is attracting attention as a microbial sterilization technology at the same time as chemical treatment. Atmospheric pressure plasma technology capable of generating plasma at low temperature under atmospheric pressure is divided into atmospheric pressure plasma and reduced pressure plasma. The reduced pressure plasma has advantages in that it is easy to control the plasma generation rate and can generate a uniform plasma, but it requires a facility to lower the pressure to a state close to vacuum, and has disadvantages in that continuous processing is difficult. On the other hand, atmospheric pressure plasma generates plasma under atmospheric pressure, so the advantages are simple equipment, continuous processing, and low equipment cost.

On the other hand, the discharge form of atmospheric pressure plasma includes dielectric barrier discharge (DBD), corona discharge, microwave discharge, arc discharge, etc., and in the present invention, there are very large criticisms. The dielectric barrier discharge method that operates under the type conditions, is capable of high-output discharge, does not have an electric shock, and can handle a large area was used.

In the present invention, the plasma intensity is 150 to 300 W/cm 2 , preferably 200 to 250 W/cm 2 . When the plasma intensity is less than the lower limit, the effect of improving bioabsorption is insignificant, and when the plasma intensity exceeds the upper limit, the sterilization effect may increase, but protein denaturation and bioabsorption according to the temperature increase may rather decrease.

In the present invention, the plasma treatment time, that is, the contact time of the plasma and the collagen powder is 1 to 5 minutes, preferably 2 to 4 minutes. When the contact time is less than the lower limit, the effect of improving bioabsorption is insignificant, and when the upper limit is exceeded, the sterilization effect may increase, but protein denaturation and bioabsorption according to the temperature increase may rather decrease.

The gas for discharging the atmospheric pressure plasma may be nitrogen, clean air, or a mixture thereof.

The gas flow rate of the atmospheric pressure plasma may be 5000 to 30000 sccm, and the electrode of the atmospheric pressure plasma may be a direct electrode.

The plasma intensity and time are based on a distance of 6 cm between the powder and the atmospheric pressure plasma electrode.

Before the plasma treatment, the step of adjusting the moisture content of the collagen powder to 8 to 20% by weight, preferably 12 to 16% by weight; may further include. The moisture content of a conventional collagen powder is 10% by weight or less, preferably 8% by weight or less, and more preferably 6% by weight or less. In the present invention, it is advantageous to increase the moisture content of the collagen powder to a certain level or more by adding water or an aqueous organic acid solution to the conventional collagen powder before plasma treatment to improve bioabsorption.

The method of adding water or an organic acid aqueous solution is to put the collagen powder in a tumbler or a fluidized bed granulator and spray water or organic acid aqueous solution. In the organic acid aqueous solution, citric acid, lactic acid, etc. that can be used for food may be used as the organic acid, and the organic acid aqueous solution is 1 to 5% by weight, preferably 1.5 to 3% by weight. It is preferable because it can increase the bioabsorption while shortening the.

After the plasma treatment, drying the collagen powder with superheated steam; may include more.

The drying with superheated steam improves bioabsorption while drying the moisture content of the powder to 10% by weight or less by contacting the collagen powder at a superheated steam temperature of 150 to 400° C. for 10 seconds to 3 minutes.

The present invention also relates to a food composition comprising the collagen powder with improved bioabsorption.

In the food composition of the present invention, the collagen powder may be formulated in the form of a capsule, tablet, powder, granule, liquid, pill, piece, paste, syrup, gel, jelly or bar, or beverage, tea , spices, gum, confectionery, etc., manufactured in the form of general food by adding it to food materials, and it means that when ingested, it brings a specific effect on health. It has the advantage that there are no side effects that may occur during operation.

The food composition is very useful because it can be ingested on a daily basis. The amount of collagen powder added in such a food composition varies depending on the type of target food and cannot be uniformly defined, but it may be added within a range that does not impair the original taste of the food, and is usually 0.01 to 50 weight by weight relative to the target food. %, preferably in the range of 0.1 to 20% by weight. In addition, in the case of food in the form of capsules, tablets, powders, granules, liquids, pills, flakes, pastes, syrups, gels, jellies or bars, usually 0.1 to 100% by weight, preferably 0.5 to 80% by weight It can be added within the range.

The food composition may include not only collagen powder as an active ingredient, but also ingredients commonly added during food production, for example, proteins, carbohydrates, fats, nutrients, seasonings and flavoring agents. Examples of the above-mentioned carbohydrates include monosaccharides such as glucose, fructose and the like; disaccharides such as maltose, sucrose, oligosaccharides and the like; and polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin, and the like, and sugar alcohols such as xylitol, sorbitol, and erythritol.

As flavoring agents, natural flavoring agents [taumatine, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.)) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used. For example, when the food composition of the present invention is prepared as a drink or beverage, citric acid, high fructose, sugar, glucose, acetic acid, malic acid, fruit juice, and various plant extracts may be additionally included in addition to the collagen powder of the present invention.

In addition, the present invention relates to a cosmetic composition comprising the collagen powder with improved bioabsorbability.

In the cosmetic composition of the present invention, the collagen powder may be included in an amount of 0.01 to 10% by weight based on the total weight of the cosmetic composition. When the content of the collagen powder falls within the above-described range, atopic relief and improvement effects can be significantly exhibited.

On the other hand, the cosmetic composition of the present invention includes ingredients commonly used in cosmetic compositions in addition to including the above-mentioned ingredients as active ingredients. For example, conventional antioxidants, stabilizers, solubilizers, vitamins, pH adjusters, colorants, flavoring agents, pigments, fragrances, bactericides, preservatives, humectants, thickeners, diluents, surfactants, alcohols, neutralizers, softeners, It further includes conventional adjuvants and carriers such as conditioning agents, excipients, inorganic salts, synthetic polymers, and the like.

In addition, their content may be appropriately adjusted according to the purpose of the final product and its purpose of use. For example, as the carrier, purified water, monohydric alcohols (ethanol or propyl alcohol), polyhydric alcohols (glycerol, 1,3-butyrene glycol or propylene glycol), higher fatty acids (palmitylic acid or linolenic acid), oils and fats (wheat) Germ oil, camellia oil, jojoba oil, olive oil, squalene, sunflower oil, macadamia peanut oil, avoguard oil, or fatty acid glyceride) may be used, but is not particularly limited thereto.

Also, non-limiting examples of surfactants that can be used include anionic surfactants, cationic surfactants, nonionic surfactants, amphoteric surfactants, and the like. More specifically, as the anionic surfactant, alkylbenzene sulfonate, polyoxyalkylene alkyl sulfate ester, alkyl sulfate ester, olefin sulfonate, alkyl phosphate, polyoxyalkylene alkyl ether phosphate, dialkyl sulfosuccinic acid salt and acid salts and fatty acid salts. Examples of the nonionic surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyhydric alcohol fatty acid partial ester, polyoxyethylene polyhydric alcohol fatty acid partial ester, polyglycerol fatty acid ester, and polyoxyethylene fatty acid ester. An ethylene hydrogenated castor oil derivative, fatty acid diethanolamide, etc. are mentioned. Examples of the cationic surfactant include tertiary aliphatic amine salts, alkyltrimethylammonium halide, and dialkyldimethylammonium halide, and examples of the amphoteric surfactant include amide betaine, imidazolinium betaine, and sulfobetaine. and the like.

As said moisturizing agent, glycerol, propylene glycol, 1, 3- butylene glycol, dipropylene glycol, sorbitol, etc. are mentioned. Benzoic acid, dehydroacetic acid, paraoxybenzoic acid ester (methyl paraoxybenzoate, butyl paraoxybenzoate, etc.), phenoxyethanol etc. are mentioned as said preservative. Moreover, as said antioxidant, ascorbic acid, BHA, etc. are mentioned, In addition, a ultraviolet absorber, an anti-inflammatory agent, a refreshing agent, etc. can be added.

The cosmetic composition may be prepared in any formulation conventionally prepared in the art, for example, lotion, emulsion, gel, cream, essence, pack, ampoule, lotion, cleaning agent, soap, body products, soap, oil, lipstick , may be in any one formulation selected from spray, powder and foundation. More specifically, it has the formulation of a flexible lotion (skin), astringent lotion, nourishing lotion (milk lotion), nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder. can

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additional chlorofluorohydrocarbon, propane /may contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol fatty esters, fatty acid esters of polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, and microcrystals Adult cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide as carrier components Ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester and the like may be used.

The cosmetic composition of the present invention can be used daily and can also be used for an indefinite period. Preferably, according to the age, skin condition or skin type of the user, and the concentration of collagen, the amount of use, the number of times of use, and the period can be appropriately adjusted.

Hereinafter, the present invention will be described in more detail through examples, etc., but the scope and content of the present invention cannot be construed as reduced or limited by the examples below.

Preparation Example: Preparation of plasma-treated collagen powder

As collagen powder extracted from fish scales, collagen powder 1 having an average molecular weight of 2000 Da and collagen powder 2 having an average molecular weight of 400 Da were used as raw materials. The collagen powders 1 and 2 have a water content of 5.4 to 5.8% by weight, a pH of a 10 w/w% aqueous solution of 5.6 to 6.0, and a refractive index (nD20) in the range of 1.32 to 1.36.

As the plasma processing apparatus, an atmospheric pressure plasma processing apparatus of the dielectric barrier discharge method was used, and clean air was used as the discharge gas.

After fixing the treatment time to 3 minutes in the plasma processing apparatus, the plasma intensity was treated at 0, 50, 150, 250, 350, 450, 550 and 650 W/cm 2 .

Meanwhile, the plasma intensity was fixed at 200 W/cm 2 and the treatment time was 0 sec, 30 sec, 1 min 30 sec, 3 min, 4 min 30 sec, 6 min, and 7 min 30 sec.

Experimental Example 1: Water solubility evaluation

The dissolution rate was evaluated in order to evaluate the solubility in water of the collagen powder samples of Preparation Example. The dissolution rate was a cylindrical tube with an inner diameter of 10 mm, a length of 40 mm, and a capacity of 2 mL, mixed with 10 mg of sample and 1 mL of distilled water, and sealed, and mixed while turning 180° 20 times per minute. Part of the samples were sampled at 2 minutes, 4 minutes, 6 minutes, 8 minutes, 10 minutes, 15 minutes, 20 minutes, and 30 minutes while mixing was started, and the collagen concentration of the supernatant was calculated by centrifugation at 5,000 rpm. Next, the dissolution rate (mg/min) was calculated from the relationship between the elapsed time and the collagen concentration. In addition, the dissolution rate was calculated as the linear time within 10 minutes from the start of mixing.

plasma intensity
(W/cm2) Collagen Powder 1
(average molecular weight 2000 Da) Collagen Powder 2
(average molecular weight 400 Da) 0 0.21 0.25 50 0.24 0.29 150 0.54 ^a 0.62 ^b 250 0.72 ^b 0.81 ^b 350 0.34 0.38 ^a 450 0.19 0.25 550 0.15 0.18 650 0.08 0.12

When the plasma treatment time was 3 minutes, it was confirmed that the dissolution rate at the plasma intensity of 150 and 250 W/cm 2 was significantly increased by 2-3 times compared to the collagen powder control (0 W) not treated with plasma.

processing time
(minute) Collagen Powder 1
(average molecular weight 2000 Da) Collagen Powder 2
(average molecular weight 400 Da) 0 0.21 0.25 0.5 0.35 0.38 1.5 0.52 ^a 0.58 ^a 3.0 0.66 ^b 0.74 ^b 4.5 0.62 ^b 0.58 ^a 6.0 0.42 0.42 7.5 0.24 0.33

When the plasma intensity is 200 W/cm2, it can be seen that the dissolution rate is significantly increased 2-3 times compared to the collagen powder control without plasma treatment (0 minutes) between 1.5 to 6.0 minutes, preferably 3.0 to 4.5 minutes. there was.

Experimental Example 2: Evaluation of the effect of promoting collagen synthesis in skin fibroblasts

The mechanism of collagen synthesis in skin fibroblasts is largely: ① Collagen gene transcription initiation, ② Transcription (mRNA synthesis) [Transcription (mRNA synthesis)] ③ Translation (Translation (protein synthesis)], ④ Translation Collagen is synthesized through the five-step protein gene expression process of post-translational modifications, ⑤ secretion and further modification.

When the bioabsorbability of collagen is increased, for example, when taken orally, it is immediately absorbed from the intestine to increase the blood transfer rate, and when applied to the skin, the skin permeability is increased to effectively promote collagen synthesis.

Therefore, in order to confirm the bioabsorption of collagen, Human Dermal Fibroblast (HDF) was treated with 10 v/v% Fetal Bovine Serum (FBS) and Dulbeccos's Minimum Essential Essential with 1 v/v% penicillin and streptomycin added. Culture in medium (DMEM) to maintain passage number 5 or less, and to test the change in the amount of collagen synthesis according to the collagen powder treatment of the preparation example in vitro , to evaluate the effect of promoting collagen synthesis in human skin fibroblasts did

For each sample, human skin fibroblasts were inoculated into a well plate at a density of 1 x 10 ⁴ cells/cm ² , and then placed in an incubator (37 °C, 5% CO ₂ ) for 24 hours for cell adhesion and environmental adaptation. cultured. And after removing the medium, the medium was treated with no serum and starvated for 8 hours. The collagen powder was dissolved in a medium at 100 μg/mL and treated for each sample. After sample treatment, human skin fibroblasts were cultured for 72 hours, and the supernatant of the medium was taken to quantify the synthesized collagen. Collagen quantification was performed using a Type I Procollagen C-Peptide EIA kit (Takara, Japan).

When the experimental group treated only with medium to which collagen powder was not added was taken as a negative control and the procollagen concentration of the negative control was 100.0%, the procollagen concentration of each sample treatment group was expressed as a relative percentage, and the The collagen content is shown in Table 3, and the collagen content according to the treatment time is shown in Table 4.

plasma intensity
(W/cm2) Collagen Powder 1
(average molecular weight 2000 Da) Collagen Powder 2
(average molecular weight 400 Da) 0 108.2 115.4 50 108.5 120.8 150 122.6 ^a 135.6 ^b 250 131.9 ^b 155.4 ^b 350 114.4 128.2 ^b 450 107.7 117.6 550 89.0 107.4 650 72.7 101.1

When the plasma treatment time was 3 minutes, it was confirmed that the procollagen concentration, that is, the collagen synthesis rate, was significantly increased at the plasma intensity of 150 and 250 W/cm 2 compared to the collagen powder control (0 W) not treated with plasma.

processing time
(minute) Collagen Powder 1
(average molecular weight 2000 Da) Collagen Powder 2
(average molecular weight 400 Da) 0 108.2 115.4 0.5 109.6 116.0 1.5 119.7 ^a 122.4 ^a 3.0 124.9 ^a 132.4 ^b 4.5 122.4 ^a 133.4 ^b 6.0 112.4 124.5 ^a 7.5 101.5 110.4

When the plasma intensity was 200 W/cm 2 , it was confirmed that the collagen synthesis rate significantly increased between 1.5 to 6.0 minutes, preferably 3.0 to 4.5 minutes, compared to the collagen powder control without plasma treatment (0 minutes).

Experimental Example 3: Cytotoxicity evaluation

In HEL 299, Clone M-3 and CCD-986sk 3 cells, a negative control (collagen powder 2) without plasma treatment and collagen powder 2 (Example 1) treated for 3 minutes when the plasma intensity was 200 W/cm 2 After treatment with various concentrations from 0.2 mg/mL to 1.0 mg/mL, and cultured for 48 hours, the cell viability was observed by MTT method, and is shown in Table 5.

division density
(mg/mL) HEL299
Cell viability % Melan-a
Cell viability % CCD-986sk
Cell viability % control - 100 100 100 Example 1 0.5 100.1 100.1 100.4 One 102.1 100.2 102.7 5 100.8 100.6 103.2 50 103.1 99.5 102.7

As shown in Table 5, it was confirmed that the plasma-treated collagen powder of Example 1 did not show cytotoxicity even at the highest concentration of 50 mg/mL. That is, it was confirmed that the plasma-treated collagen powder of Example 1 of the present invention did not induce other side effects and toxicity to cells.

Experimental Example 4: Evaluation of skin moisturizing power

In the essence of Formulation Example 8 (Example 2), in the essence of Formulation Example 8, a control in which collagen powder 2 without plasma treatment was added instead of plasma-treated collagen powder 2 of Example, and purified water was added instead of collagen powder at all In order to confirm the effect of increasing skin moisturizing power of one negative control group, the amount of skin moisture was measured.

After washing the faces of 20 adult men and women in their 40s and 50s classified as dry skin, they were dried with hot air and the cosmetic compositions of Example 2, negative control group and control group were applied. Immediately after washing, after hot air drying, and immediately after application, the skin moisture content was measured with a skin moisture meter (Corneometer CM825, C+K Electronic Co., Germany) under constant temperature and constant humidity conditions (24°C, 40% relative humidity) and shown in Table 6. it was

division skin moisture content immediately after washing After hot air drying immediately after application negative control 45.721 31.870 31.830 control 45.730 30.567 42.320 Example 2 45.723 32.567 56.794

It can be seen that the cosmetic composition containing the plasma-treated collagen powder of Example 2 of the present invention maintains a high level of moisture content even after drying.

The significance test of the experimental results was performed by one way analysis of variance (ANOVA), and Turkey's HDS method was used for post-hoc testing between groups. A P value of 0.05 or less was considered statistically significant. A p-value less than 0.05 was denoted as a superscript a, and a value less than 0.01 was denoted as a superscript b.

Hereinafter, a formulation example of a composition containing the extract of the present invention will be described, but the present invention is not intended to limit it, but to describe it in detail.

Formulation Example 1: Preparation of powder

20 mg of plasma-treated collagen powder of Example 1

Lactose 100 mg

talc 10 mg

The above ingredients are mixed and filled in an airtight bag to prepare a powder.

Formulation Example 2: Preparation of tablets

10 mg of plasma-treated collagen powder of Example 1

100 mg cornstarch

Lactose 100 mg

2 mg magnesium stearate

After mixing the above ingredients, tablets are prepared by tableting according to a conventional manufacturing method of tablets.

Formulation Example 3: Preparation of capsules

10 mg of plasma-treated collagen powder of Example 1

3 mg of crystalline cellulose

Lactose 14.8 mg

0.2 mg magnesium stearate

According to a conventional capsule preparation method, the above ingredients are mixed and filled in a gelatin capsule to prepare a capsule.

Formulation Example 4: Preparation of granules

1,000 mg of plasma-treated collagen powder of Example 1

appropriate amount of vitamin mixture

70 μg vitamin A acetate

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

0.5 mg of vitamin B6

0.2 μg of vitamin B12

Vitamin C 10 mg

Biotin 10 μg

Nicotinamide 1.7 mg

50 μg of folic acid

Calcium pantothenate 0.5 mg

Mineral mixture appropriate amount

ferrous sulfate 1.75 mg

Zinc Oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Dibasic calcium phosphate 55 mg

Potassium citrate 90 mg

100 mg of calcium carbonate

Magnesium chloride 24.8 mg

The composition ratio of the vitamin and mineral mixture is mixed with ingredients suitable for health functional food in a preferred embodiment, but the mixing ratio may be arbitrarily modified, and the ingredients are mixed according to a conventional health functional food manufacturing method. Next, the granules can be prepared and used in the preparation of a health functional food composition according to a conventional method.

Formulation Example 5: Preparation of beverage formulations

1,000 mg of plasma-treated collagen powder of Example 1

1,000 mg citric acid

100 g of oligosaccharides

2 g of plum concentrate

1 g taurine

Add purified water to total 900 mL

After mixing the above ingredients according to a conventional beverage manufacturing method, after stirring and heating at 85 ° C. for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed and sterilized, and then refrigerated. used in the manufacture of functional beverage compositions.

Formulation Example 6: Preparation of nutritional lotion

Plasma-treated collagen powder of Example 1 0.05 wt%

2.0 wt% petrolatum

Sesquioleate sorbitan 0.8 wt%

1.2 wt% of polyoxyethylene oleylethyl

Appropriate amount of methyl paraoxybenzoate

Propylene glycol 5.0 wt%

3.2% by weight of ethanol

18.0 wt% of carboxyvinyl polymer

Potassium hydroxide 0.1% by weight

proper amount of color

incense

Nutritional lotion was prepared by mixing the above ingredients according to a conventional nutritional lotion manufacturing method.

Formulation Example 7: Preparation of a mask pack

Plasma-treated collagen powder of Example 1 0.05 wt%

glycerin 5.0% by weight

propylene glycol 4.0 wt%

15.0 wt% of polyvinyl alcohol

Ethanol 8.0 wt%

1.0 wt% of polyoxyethylene oleyl ethyl

0.2 wt% of methyl paraoxybenzoate

appropriate amount of color

incense

A mask pack was prepared by mixing the above ingredients according to a conventional mask pack manufacturing method.

Formulation Example 8: Preparation of Essence

0.2 wt% of the plasma-treated collagen powder of Example 1

Propylene glycol 10.0 wt%

10.0 wt% glycerin

5.0 wt% of sodium hyaluronate aqueous solution (1%)

ethanol 5.0% by weight

1.0 wt% of polyoxyethylene hydrogenated castor oil

0.1 wt% of methyl paraoxybenzoate

incense

Purified water remaining

Essence was prepared by mixing the above ingredients according to a conventional essence preparation method.

Claims (8)

Putting the collagen powder into a tumbler or a fluidized bed granulator and spraying 1 to 5% by weight of an organic acid aqueous solution to adjust the moisture content of the collagen powder to 12 to 16% by weight;
Atmospheric pressure plasma treatment of the collagen powder with the moisture content controlled at 150 to 300 W plasma intensity for 1 to 5 minutes; and
Drying the plasma-treated collagen powder with superheated steam; A method for producing collagen powder with improved bioabsorbability, comprising: According to claim 1,
In the atmospheric pressure plasma treatment step,
The gas for discharge is nitrogen, clean air, or a mixture thereof,
The gas flow rate is 5000 to 30000 sccm,
The electrode is a direct-type electrode,
The plasma intensity and time is a method for producing collagen powder with improved bioabsorption, characterized in that the distance between the powder and the atmospheric pressure plasma electrode is 6 cm. According to claim 1,
The organic acid aqueous solution is citric acid or lactic acid aqueous solution, and the concentration of the organic acid aqueous solution is 1.5 to 3% by weight. According to claim 1,
In the step of drying with superheated steam, the collagen powder with improved bioabsorption, characterized in that the moisture content of the powder is dried to 10% by weight or less by contacting the collagen powder with a superheated steam temperature of 150 to 400° C. for 10 seconds to 3 minutes. manufacturing method. 5. The method according to any one of claims 1 to 4,
The molecular weight of the collagen powder is a method for producing a collagen powder with improved bioabsorption, characterized in that 300 to 5000 Da. delete delete delete