KR102323878B1 - Cosmetic composition for anti-inflammation and manufacturing method for the same - Google Patents

Abstract

The present invention relates to a cosmetic composition for anti-inflammation and a method for manufacturing the same, and specifically relates to a cosmetic composition for anti-inflammation capable of improving an anti-inflammation effect by adjusting components included in the cosmetic composition and contents thereof, and a method for manufacturing the same. The cosmetic composition for anti-inflammation includes a complex extract including extracts extracted from dried products of Angelica sinensis, Mentha arvensis, Morus Alba leaves, Sasa borealis, Gardeniae Fructus, and Liriope muscari.

Description

Cosmetic composition for anti-inflammatory and manufacturing method thereof

The present invention relates to an anti-inflammatory cosmetic composition and a method for manufacturing the same, and more particularly, to an anti-inflammatory cosmetic composition capable of improving the anti-inflammatory effect by adjusting the components and content contained in the cosmetic composition, and to a method for preparing the same.

Recently, due to changes in diet and living environment, interest in free radicals involved in various adult diseases and aging is increasing. Oxygen in a stable state in the human body is a highly reactive reactive oxygen species such as superoxide anion radical, hydrogen peroxide radical, and singlet oxygen through biochemical mechanisms. ) is converted to

^{Superoxide radical anion (O 2 -} ·), hydrogen peroxide (H ₂ O ₂ ), hydroxyl radical generated in the course of normal intracellular activity in vivo or by environmental pollution or exposure to chemicals (hydroxylradicals, ·OH) are called reactive oxygen species (ROS). Active oxygen means oxygen with free radicals, has an unstable nature, and is known to cause oxidative stress in cells due to its strong reactivity with surrounding substances. Active oxygen, such as superoxide anion radical, hydrogen peroxide radical, and singlet oxygen, promotes oxidation in vivo, causes peroxidation of unsaturated fatty acids in phospholipids, enhances cell membrane permeability, and causes cytotoxicity, leading to aging, inflammation and various induce the pathology of the disease. Currently, various studies are being conducted on natural materials that protect cells from oxidative promotion of free radicals and materials that can protect living bodies by regulating the expression of proteins involved in inflammation.

The plant complex extract was confirmed to have excellent antioxidant activity in vitro and anti-inflammatory effect in cell model experiments through cosmetic pharmacological activity tests, but its activity was not significantly noticed.

Accordingly, the present invention provides a composition and a method for preparing the same, which can be used as a cosmetic to prevent skin aging while improving the anti-inflammatory effect by controlling the components included in the plant complex extract.

The technical problem to be achieved by the present invention is to provide an anti-inflammatory cosmetic composition capable of improving the anti-inflammatory effect by controlling the components and content of the extract included in the cosmetic composition, and a method for preparing the same.

However, the problems to be solved by the present invention are not limited to the above-mentioned problems, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

One embodiment of the present invention is Angelicasinensis ), peppermint ( Mentha arvensis var. piperascens ), upper leaves ( Morusalba leaves ), bamboo leaves ( Sasa borealis ), gardenia ( Gardeniae Fructus ) Plant complex extract comprising an extract extracted from each dried product It provides an anti-inflammatory cosmetic composition comprising as an active ingredient.

According to an exemplary embodiment of the present invention, the extract may be extracted with an extraction solvent selected from the group consisting of water, an organic solvent, and a mixed solvent thereof.

According to an exemplary embodiment of the present invention, the organic solvent may be one selected from the group consisting of alcohols having 1 to 4 carbon atoms, ethyl acetate, glycerol, ethylene glycol, propylene glycol, butylene glycol, and combinations thereof.

According to an exemplary embodiment of the present invention, the Angelica extract may be extracted from the dried Angelica plant in an amount of 50 parts by weight or more and 200 parts by weight or less based on 100 parts by weight of the dried Gardenia dried product.

According to an exemplary embodiment of the present invention, the mint extract may be extracted from the dried mint in an amount of 150 parts by weight or more and 250 parts by weight or less based on 100 parts by weight of the dried gardenia.

According to an exemplary embodiment of the present invention, the upper leaf extract may be extracted from the dried upper leaves in an amount of 50 parts by weight or more and 150 parts by weight or less based on 100 parts by weight of the dried gardenia.

According to an exemplary embodiment of the present invention, the bamboo leaf extract may be extracted from the dried bamboo leaves in an amount of 150 parts by weight or more and 250 parts by weight or less based on 100 parts by weight of the dried gardenia.

According to an exemplary embodiment of the present invention, it may further include water, and the content of the plant complex extract may be 1% by weight or more and 10% by weight or less.

According to an exemplary embodiment of the present invention, it may further include a cosmetic base composition.

An exemplary embodiment of the present invention is a process for preparing a cosmetic composition for the anti-inflammatory, Angelica (Angelicasinensis), mint (Mentha arvensis var. Piperascens), the upper lobe (Morusalba leaves), jukyeop (Sasa borealis), gardenia (GardeniaeFructus) preparing each mixture by mixing each dried product with an extraction solvent; heating each mixture to extract each extract; preparing a plant complex extract by mixing the respective extracts; and mixing the plant complex extract with a cosmetic base composition to provide a method for producing a cosmetic composition for anti-inflammatory comprising the step of preparing a cosmetic composition.

According to an exemplary embodiment of the present invention, the temperature of the heating step may be 40 ℃ or more and 80 ℃ or less.

According to an exemplary embodiment of the present invention, the heating step may be applying an ultrasonic wave of 700 kHz or more and 1,200 kHz or less.

The anti-inflammatory cosmetic composition according to an exemplary embodiment of the present invention can improve the anti-inflammatory effect.

The method for producing a cosmetic composition for anti-inflammatory according to an exemplary embodiment of the present invention can easily prepare the cosmetic composition while maximizing the anti-inflammatory effect implemented in the cosmetic.

Effects of the present invention are not limited to the above-described effects, and effects not mentioned will be clearly understood by those skilled in the art from the present specification and accompanying drawings.

1 is a flowchart of a method for manufacturing an anti-inflammatory cosmetic composition according to an exemplary embodiment of the present invention.
2 is a graph showing the relative comparison of cell viability according to the amount of sample added in Example.
3 is a graph showing the amount of inflammatory cytokines generated according to the amount of sample added in Example.

Throughout this specification, when a part "includes" a certain element, it means that other elements may be further included, rather than excluding other elements, unless otherwise stated.

Throughout this specification, the unit “part by weight” may mean a ratio of weight between each component.

Throughout this specification, "A and/or B" means "A and B, or A or B."

Throughout this specification, the term "extract" includes all substances obtained by extracting components therein from natural products, regardless of the extraction method or types of components. For example, it may mean extracting a component soluble in a solvent from a natural product using water or an organic solvent.

Hereinafter, the present invention will be described in more detail.

One embodiment of the present invention is Angelicasinensis ), peppermint ( Mentha arvensis var. piperascens ), upper leaves ( Morusalba leaves ), bamboo leaves ( Sasa borealis ), gardenia ( Gardeniae Fructus ) Plant complex extract comprising an extract extracted from each dried product It provides an anti-inflammatory cosmetic composition comprising as an active ingredient.

The anti-inflammatory cosmetic composition according to an exemplary embodiment of the present invention can improve the anti-inflammatory effect.

According to an exemplary embodiment of the present invention, the anti-inflammatory cosmetic composition includes an extract extracted from a dried product of Angelicasinensis. Specifically, Angelica (Angelicasinensis) is a perennial plant belonging to the Apiaceae, one shot, we used the roots in medicine. Angelica is effective for people who do not have blood on their face, often get dizziness, and do not have gloss on their eyes and lips, because it has the effect of replenishing blood. Therefore, Angelica quai is often used for various diseases before or after childbirth because of its excellent efficacy in controlling headaches and menstruation in women, and it treats menstrual irregularities and abdominal pain. In addition, it is effective in treating constipation due to weak stomach function. As described above, since the anti-inflammatory cosmetic composition contains an extract extracted from the dried angelica root, it is possible to alleviate inflammation occurring in the body.

According to an exemplary embodiment of the present invention, the anti-inflammatory cosmetic composition includes an extract extracted from the dried matter of mint (Mentha arvensis var. piperascens). Specifically, peppermint ( Mentha arvensis var. piperascens ) is a dicotyledonous perennial succulent plant in the order Lamiaceae. The main components of mint are alpha-pinene (+, -), anisaldehyde, Daucosterol, Eugenol, Linalool, Menthone, (+)-Neomenthol ((+)-Neomenthol) and the like. Mint emits a refreshing and refreshing scent, and has sterilizing and antibacterial action against pathogenic fungi, Escherichia coli, and Staphylococcus aureus that cause eczema and scabies on the skin and scalp. As described above, since the anti-inflammatory cosmetic composition contains the extract extracted from the dried mint of mint, it is possible to improve the sterilization and antibacterial action of the cosmetic composition, thereby alleviating inflammation occurring in the body.

According to an exemplary embodiment of the present invention, the anti-inflammatory cosmetic composition includes an extract extracted from the dried matter of Morusalba leaves. Specifically, "upper leaf" refers to the leaves of Morusalba leaves , and in oriental medicine may refer to drugs obtained by drying the leaves of Morusalba leaves. The upper leaves are used in folk remedies to treat various diseases such as beriberi, edema, and diabetes. show the effect. As described above, the anti-inflammatory cosmetic composition includes an extract extracted from the dried matter of the upper leaf, thereby improving the antibacterial activity of the cosmetic composition, thereby alleviating inflammation occurring in the body or skin.

According to an exemplary embodiment of the present invention, the anti-inflammatory cosmetic composition includes an extract extracted from the dried matter of bamboo leaves ( Sasa borealis ). Specifically, the bamboo leaf ( Sasa borealis ) is a dried leaf of chamdae (or royal daisy), which is bitter in taste and has the effect of lowering body heat as a cold medicine. The bitter and cold nature of bamboo leaves lowers heat in the heart and lungs, and the spicy taste disturbs the wind and heat of sage and prevents hair loss due to stress in the body. As described above, by including the extract extracted from the dried bamboo leaves of the anti-inflammatory cosmetic composition, the cosmetic composition lowers the heat of the skin to prevent the inflammation occurring in the skin from worsening.

According to an exemplary embodiment of the present invention, the anti-inflammatory cosmetic composition includes an extract extracted from a dried product of Gardeniae Fructus. Specifically, "Gardenia ( GardeniaeFructus )" is a plant belonging to the family Rubiaceae, and the main components are Crocin, Crocetin, Gardonin, Genipin, Geniposide, etc. The yellow pigment contains components such as alpha crotine, xanthyltin, psoralen, fauchedanolmethyl, and esters. Gardenia is used in folk remedies for bruises and wounds, and it is effective for fever, blood loss, anti-inflammation, hematemesis, hemoptysis, and jaundice. As described above, since the anti-inflammatory cosmetic composition includes an extract extracted from the dried matter of Gardeniae Fructus , it is possible to minimize inflammation in the skin due to the anti-inflammatory effect.

According to an exemplary embodiment of the present invention, the term cosmetic composition for inflammation Angelica (Angelicasinensis) extract, peppermint (Mentha arvensis var. Piperascens) extract, mulberry leaf (Morusalba leaves) extract, jukyeop (Sasa borealis) extract, gardenia (GardeniaeFructus) plant complex extracts including extracts. Wherein the anti-inflammatory cosmetic composition for Angelica (Angelicasinensis) as described above extract, peppermint (Mentha arvensis var. Piperascens) extract, mulberry leaf (Morusalba leaves) extract, jukyeop (Sasa borealis) extract, gardenia (GardeniaeFructus) plant containing the extract By including the complex extract, it is possible to prevent the anti-inflammatory effect of the anti-inflammatory cosmetic composition from being lowered by other ingredients and minimize inflammation occurring on the skin.

According to an exemplary embodiment of the present invention, the anti-inflammatory cosmetic composition may further include one selected from the group consisting of maekmundong extract, mulberry extract, cornus extract, self-inflicted extract, cheonmundong extract, hwangbaek extract, and combinations thereof. By further including the components of the anti-inflammatory cosmetic composition from the above-mentioned extract, the effect to be additionally implemented in the anti-inflammatory cosmetic composition can be implemented.

According to an exemplary embodiment of the present invention, the extract may be extracted with an extraction solvent selected from the group consisting of water, an organic solvent, and a mixed solvent thereof. The by extracting from those described above by selecting the extraction solvent, the Angelica (Angelicasinensis) Structures, mint (Mentha arvensis var. Piperascens) dried material, reconstituted tobacco (Morusalba leaves) and Structures, jukyeop (Sasa borealis) dried material and gardenia (GardeniaeFructus) dry matter The included active ingredients can be extracted as much as possible.

According to an exemplary embodiment of the present invention, the organic solvent may be one selected from the group consisting of alcohols having 1 to 4 carbon atoms, ethyl acetate, glycerol, ethylene glycol, propylene glycol, butylene glycol, and combinations thereof. The alcohol having 1 to 4 carbon atoms may be methanol, ethanol, propanol or butanol. By selecting the organic solvent from the above, it is possible to improve the efficiency of extracting the active ingredient from the dried product, and it is possible to prevent other components from being extracted other than the active ingredient.

According to an exemplary embodiment of the present invention, the angelica extract may be extracted from the dried angelica plant in an amount of 50 parts by weight or more and 200 parts by weight or less based on 100 parts by weight of the dried gardenia plant. Specifically, the angelica extract is 60 parts by weight or more and 190 parts by weight or less, 70 parts by weight or more and 180 parts by weight or less, 80 parts by weight or more and 170 parts by weight or less, 90 parts by weight or more and 160 parts by weight or less, based on 100 parts by weight of the dried gardenia. 100 parts by weight or more and 150 parts by weight or less, 110 parts by weight or more and 140 parts by weight or less, or 120 parts by weight or more and 130 parts by weight or less, may be extracted from the dried angelfish. More specifically, the Angelica root extract is preferably extracted from 130 parts by weight of the dried Angelica plant per 100 parts by weight of the dried gardenia. By adjusting the content of the dried Angelica lily in the above range, by improving the blood retention function, it is possible to improve the anti-inflammatory effect, and it is possible to prevent side effects caused by excessive use of the Angelica kei.

According to an exemplary embodiment of the present invention, the mint extract may be extracted from the dried mint in an amount of 150 parts by weight or more and 250 parts by weight or less based on 100 parts by weight of the dried gardenia. Specifically, the mint extract is contained in an amount of 160 parts by weight or more and 240 parts by weight or less, 170 parts by weight or more and 230 parts by weight or less, 180 parts by weight or more and 220 parts by weight or less, or 190 parts by weight or more and 210 parts by weight or less with respect to 100 parts by weight of the dried gardenia. It may be extracted from the dried mint. More specifically, the mint extract is preferably extracted from the dried mint in an amount of 200 parts by weight or less based on 100 parts by weight of the dried gardenia. By adjusting the content of the dried mint in the above range, sterilization and antibacterial action can be improved, and excessive antibacterial action can be prevented from occurring, thereby maximizing the anti-inflammatory effect.

According to an exemplary embodiment of the present invention, the upper leaf extract may be extracted from the dried upper leaves in an amount of 50 parts by weight or more and 150 parts by weight or less based on 100 parts by weight of the dried gardenia. Specifically, the upper leaf extract contains 60 parts by weight or more and 140 parts by weight or less, 70 parts by weight or more and 130 parts by weight or less, 80 parts by weight or more and 120 parts by weight or less, or 90 parts by weight or more and 110 parts by weight or less with respect to 100 parts by weight of the dried gardenia. It may be extracted from the dried upper leaves. More specifically, it is preferable that the upper leaf extract is extracted from the dried upper leaf in 100 parts by weight based on 100 parts by weight of the dried gardenia. By adjusting the content of the dried upper leaves in the above range, it is possible to prevent hyperglycemia by controlling the blood sugar level in the blood, and to maximize the effect of relieving inflammation in the body.

According to an exemplary embodiment of the present invention, the bamboo leaf extract may be extracted from the dried bamboo leaves in an amount of 150 parts by weight or more and 250 parts by weight or less based on 100 parts by weight of the dried gardenia. Specifically, the bamboo leaf extract is present in an amount of 160 parts by weight or more and 240 parts by weight or less, 170 parts by weight or more and 230 parts by weight or less, 180 parts by weight or more and 220 parts by weight or less, or 190 parts by weight or more and 210 parts by weight or less with respect to 100 parts by weight of the dried gardenia. It may be extracted from the dried bamboo leaves. More specifically, the bamboo leaf extract is preferably extracted from the dried bamboo leaf in 200 parts by weight based on 100 parts by weight of the dried gardenia. By adjusting the content of the bamboo leaves in the above-described range, it is possible to maximize the effect of alleviating inflammation by controlling the heat generated in the body.

According to an exemplary embodiment of the present invention, the anti-inflammatory cosmetic composition may further include water. As described above, since the anti-inflammatory cosmetic composition further contains water, the concentration of the anti-inflammatory cosmetic composition can be adjusted, and the content of the active ingredient in the anti-inflammatory cosmetic composition can be adjusted to maximize the inflammatory relief effect. can

According to an exemplary embodiment of the present invention, the content of the plant complex extract in the anti-inflammatory cosmetic composition may be 1% by weight or more and 10% by weight or less. Specifically, the content of the plant complex extract in the anti-inflammatory cosmetic composition is 2 wt% or more and 9 wt% or less, 3 wt% or more and 8 wt% or less, 4 wt% or more and 7 wt% or less, or 5 wt% or more and 6 wt% may be below. By adjusting the content of the plant complex extract in the anti-inflammatory cosmetic composition in the above range, the concentration of the anti-inflammatory cosmetic composition can be adjusted, and inflammation is relieved by adjusting the content of the active ingredient in the anti-inflammatory cosmetic composition effect can be maximized.

According to an exemplary embodiment of the present invention, the anti-inflammatory cosmetic composition may further include a cosmetic base composition. Specifically, the anti-inflammatory cosmetic composition may further include one selected from the group consisting of a cosmetic base composition, such as essence, cream, lotion, toner, and combinations thereof. By further including the cosmetic base composition at the same time as selecting the cosmetic base composition as described above, the active ingredient contained in the cosmetic can be well absorbed into the body, and the basic function of cosmetics by further including the ingredient can have it

An exemplary embodiment of the present invention is a process for preparing a cosmetic composition for the anti-inflammatory, Angelica (Angelicasinensis), mint (Mentha arvensis var. Piperascens), the upper lobe (Morusalba leaves), jukyeop (Sasa borealis), gardenia (GardeniaeFructus) preparing each mixture by mixing each dried product with an extraction solvent; heating each mixture to extract each extract; preparing a plant complex extract by mixing the respective extracts; and mixing the plant complex extract with a cosmetic base composition to provide a method for producing a cosmetic composition for anti-inflammatory comprising the step of preparing a cosmetic composition.

The method for producing a cosmetic composition for anti-inflammatory according to an exemplary embodiment of the present invention can easily prepare the cosmetic composition while maximizing the anti-inflammatory effect implemented in the cosmetic.

1 is a flowchart of a method for manufacturing an anti-inflammatory cosmetic composition according to an exemplary embodiment of the present invention. Hereinafter, a method of manufacturing the anti-inflammatory cosmetic composition of the present invention will be described with reference to FIG. 1 . In the manufacturing method of the anti-inflammatory cosmetic composition according to an exemplary embodiment of the present invention, a portion overlapping with the description of the anti-inflammatory cosmetic composition will be omitted.

According to an exemplary embodiment of the present invention, Angelicasinensis ), peppermint ( Mentha arvensis var. piperascens ), upper leaves ( Morusalba leaves ), bamboo leaves ( Sasa borealis ), gardeniae ( GardeniaeFructus ) Each dried product is mixed with an extraction solvent and each and preparing a mixture (S10). As described above, by extracting each dried product of Angelica chia, mint, sangyeop, bamboo leaf, and gardenia, the active ingredient contained in each dried product can be efficiently extracted.

According to an exemplary embodiment of the present invention, heating each mixture comprises the step of extracting each extract (S30). It is possible to efficiently extract the active ingredients contained in each of the dried substances. As described above, by extracting each extract by heating each mixture as described above, the inflammatory effect can be maximized by extracting at a suitable temperature so that the active ingredient of each dried product is not denatured.

According to an exemplary embodiment of the present invention, it includes the step (S50) of preparing a plant complex extract by mixing each of the extracts. By mixing the respective extracts as described above to prepare a plant complex extract, the components in the plant complex extract do not react with each other and the active ingredients are maintained while improving compatibility.

According to an exemplary embodiment of the present invention, a plant complex extract can be prepared by preparing a dry mixture of Angelica basil, dried mint, dried upper leaves, dried bamboo leaves, and dried gardenia, and heating the dried mixture. As described above, by preparing a mixture of dry matter in which all of the dry matter is mixed and heating it to prepare a plant complex extract, it is possible to provide a manufacturing method suitable for mass production by shortening the manufacturing time of the plant complex extract.

According to an exemplary embodiment of the present invention, a step of preparing a cosmetic composition by mixing the plant complex extract with a cosmetic base composition (S70) is included. As described above, by mixing the plant complex extract with the cosmetic base composition to prepare a cosmetic composition, it is possible to prepare a cosmetic with improved anti-inflammatory effect.

According to an exemplary embodiment of the present invention, the temperature of the heating step may be 40 ℃ or more and 80 ℃ or less. Specifically, the temperature of the heating step is 41 ℃ or more and 79 ℃ or less, 42 ℃ or more and 78 ℃ or less, 43 ℃ or more and 77 ℃ or less, 44 ℃ or more and 76 ℃ or less, 45 ℃ or more and 75 ℃ or less, 46 ℃ or more and 74 ℃ or less. , 47 °C or higher and 73 °C or lower, 48 °C or higher 72 °C or lower, 49 °C or higher 71 °C or lower, 50 °C or higher 70 °C or lower, 51 °C or higher 69 °C or lower, 52 °C or higher 68 °C or lower, 53 °C or higher 67 °C or lower, 54 ℃ or more and 66 ℃ or less, 55 ℃ or more and 65 ℃ or less. More specifically, the temperature of the heating step is preferably 50 ℃ By controlling the temperature of the heating step in the above-described range, it is possible to maximize the extraction efficiency of the active ingredient while preventing deterioration of each extract.

According to an exemplary embodiment of the present invention, the heating step may be applying an ultrasonic wave of 700 kHz or more and 1,200 kHz or less. Specifically, in the heating step, ultrasonic waves of 750 kHz or more and 1,150 kHz or less, 800 kHz or more and 1,100 kHz or less, 850 kHz or more and 1,050 kHz or less, or 900 kHz or more and 1,000 kHz or less may be applied. By adjusting the frequency of the ultrasonic wave applied in the heating step in the above range, it is possible to maximize the extraction efficiency of the active ingredient extracted from the dried material.

According to an exemplary embodiment of the present invention, the heating step may be performed for 24 hours or more and 96 hours or less. Specifically, 25 hours or more and 95 hours or less, 30 hours or more and 90 hours or less, 35 hours or more and 85 hours or less, 40 hours or more and 80 hours or less, 45 hours or more and 75 hours or less, 50 hours or more and 70 hours or less, or 55 hours or more and 65 hours or less. may be performed with More specifically, the heating step is preferably performed for 72 hours. By controlling the execution time of the heating step in the above range, it is possible to maximize the extraction efficiency without alteration of the active ingredient.

Hereinafter, examples will be given to describe the present invention in detail. However, the embodiments according to the present invention may be modified in various other forms, and the scope of the present invention is not to be construed as being limited to the embodiments described below. The embodiments of the present specification are provided to more completely explain the present invention to those of ordinary skill in the art.

Example

For each of 13 g of dried angelfish, 5 g of dried maekmundong, 20 g of mint, 10 g of dried leaves, 20 g of dried bamboo leaves, and 10 g of dried gardenia leaves, using propylene glycol as an extraction solvent, a frequency of 50 kHz at 1 atmosphere and 50 ℃ was applied. Ultrasound was applied for 72 hours to extract the extract.

After that, each extract was mixed to prepare a solution containing the plant complex extract, and then water was added to the plant complex extract to prepare an anti-inflammatory cosmetic composition having a concentration of about 5%.

The anti-inflammatory cosmetic composition was used after drying for 3 days in a freeze dryer (FD8508S, Busan, Korea) at -80°C. The dried sample was dissolved in distilled dimethyl sulfoxide (DMSO) and sterilized by passing through a 0.22 μm syringe filter (Millipore, MA, USA).

<Experimental Example 1 - Measurement of cell viability>

RAW264.7 cells were heat-inactivated at 5% CO ₂ , 37° C. with heat-inactivated fetal bovine serum (FBS), 100 U/ml penicillin, and 100 μg/ml streptomycin (streptomycin, Invitrogen). , Carlsbad, CA, America) supplemented with high glucose DMEM (Dulbecco's modified Eagle's medium).

After culturing the RAW264.7 cells seeded in the 96-well plate for one day, the plate to which the sample prepared in Example was not added and the sample prepared in Example (hereinafter referred to as “Example sample”) were 10 Each plate treated with μg/ml, 100 μg/ml and 1000 μg/ml was incubated for 24 hours. After incubation, 50 μl of 2,3-Bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) solution was treated and incubated again for 4 hours. After completion of the culture, the absorbance was repeatedly measured at 450 nm with a microplate reader (Molecular Devices, Sunnyvale, CA, USA) three times and summarized in Table 1 below, and the average of the values obtained by measuring the absorbance of the Example sample in Table 1 It is summarized in Table 2 by dividing by %.

round 1 round 2 round 3 Example sample-free plate (NC) 3.254 3.642 3.768 Example sample 10 μg/ml addition plate (10 μg/ml) 3.25 3.678 3.548 Example sample 100 μg/ml addition plate (100 μg/ml) 3.372 3.53 3.581 Example sample 1000 μg/ml addition plate (1000 μg/ml) 3.159 3.454 3.297

1st round (%) 2nd round (%) 3rd round (%) average(%) Standard Deviation T test value Example sample
Unadded plate (NC) 91.54164 102.4569 106.0015 100 7.536515 - Example sample 10 μg/ml
addition plate (10 μg/ml) 91.42911 103.4696 99.81245 98.23706 6.172914 0.76961 Example sample 100 μg/ml addition plate (100 μg/ml) 94.86122 99.30608 100.7408 98.3027 3.065531 0.736131 Example sample 1000 μg/ml addition plate (1000 μg/ml) 88.86909 97.16804 92.75131 92.92948 4.152343 0.227758

2 is a graph showing the relative comparison of cell viability according to the amount of sample added in Example. Referring to Table 1 and Table 2 and FIG. 2, when a toxic XTT solution is added to a plate in which RAW264.7 cells are cultured, the absorbance is maintained even when the sample prepared in Example, which is the sample prepared in Example, is added. It was confirmed that the Example sample was not toxic.

<Experimental Example 2 - Anti-inflammatory experiment>

RAW264.7 cells were heat-inactivated at 5% CO ₂ , 37° C. with heat-inactivated fetal bovine serum (FBS), 100 U/ml penicillin, and 100 μg/ml streptomycin (streptomycin, Invitrogen). , Carlsbad, CA, America) supplemented with high glucose DMEM (Dulbecco's modified Eagle's medium).

After culturing RAW264.7 cells seeded in 96 well plate for one day, the samples prepared in Examples were treated at concentrations of 10 μg/ml, 100 μg/ml and 1000 μg/ml, respectively. After 1 hour, 1ug/ml of LPS (Lipopolysaccharide) was treated and cultured for 23 hours, and the supernatant was recovered. The supernatant was reacted with IL-1β, IL-6, TNF-α mouse elisa kit (koma biotech. korea) and absorbance was measured at 450 nm with a microplate reader (Molecular Devices, Sunnyvale, CA, USA), and summarized in Table 3. , The concentrations of each of IL-1β, IL-6, and TNF-α were measured from the supernatant and summarized in Table 4.

IL-1β IL-6 TNF-α Example Sample-free and LPS-free plate (NC) 0.204 0.188 0.474 Example No sample addition and LPS (1 μg/ml) addition plate (LPS) 0.41 1.462 1.411 Example sample 10 μg/ml added and LPS (1 μg/ml) added
plate (LPS+10 μg/ml) 0.356 1.366 1.408 Example Sample 100 μg/ml Addition and LPS (1 μg/ml) Addition
plate (LPS+100 μg/ml) 0.374 1.196 1.007 Example sample 1000 μg/ml added and LPS (1 μg/ml) added
plate (LPS+1000 μg/ml) 0.287 1.064 1.031 standard 0.778 0.216 0.389

IL-1β
(pg/ml) IL-6
(pg/ml) TNF-α
(pg/ml) Example Sample-free and LPS-free plate (NC) 6.746594 46.07766 161.9268 Example No sample addition and LPS (1 μg/ml) addition plate (LPS) 94.0466 793.2939 568.9901 Example sample 10 μg/ml added and LPS (1 μg/ml) added
plate (LPS+10 μg/ml) 71.16213 736.9888 567.6868 Example Sample 100 μg/ml Addition and LPS (1 μg/ml) Addition
plate (LPS+100 μg/ml) 78.79029 637.2817 393.4793 Example sample 1000 μg/ml added and LPS (1 μg/ml) added
plate (LPS+1000 μg/ml) 41.92087 559.8622 403.9057 standard 250 62.5 125

3 is a graph showing the generation amount of inflammatory cytokines according to the sample addition amount of Example. Referring to Tables 3 and 4 and FIG. 3, it was confirmed that the example sample inhibited inflammatory cytokines (IL-1β, IL-6, TNF-α) in a concentration-dependent manner as a result of measurement of inflammatory cytokines using macrophages. .

Therefore, the anti-inflammatory cosmetic composition and its manufacturing method according to an embodiment of the present invention can improve the anti-inflammatory effect and facilitate the anti-inflammatory effect by controlling the components included in the cosmetic composition and at the same time adjusting the ratio thereof. It was confirmed that a cosmetic composition can be prepared.

In the above, although the present invention has been described by way of limited embodiments, the present invention is not limited thereto, and the technical idea of the present invention and the claims to be described below by those of ordinary skill in the art to which the present invention pertains Of course, various modifications and variations are possible within the equivalent range of the range.

S10: mixture preparation step
S30: extract extraction step
S50: Plant complex extract manufacturing step
S70: Cosmetic composition manufacturing step

Claims (12)

Angelicasinensis ), peppermint ( Mentha arvensis var. piperascens ), upper leaves ( Morusalba leaves ), bamboo leaves ( Sasa borealis ), gardenia ( GardeniaeFructus ) and plant complex extracts containing extracts from each dried plant as an active ingredient. ,
The Angelica extract is
It is extracted from 130 parts by weight of the dried angelica root with respect to 100 parts by weight of the dried gardenia,
The mint extract is
It is extracted from the dried mint of 200 parts by weight based on 100 parts by weight of the dried gardenia,
The upper leaf extract,
It is extracted from the dried upper leaves of 100 parts by weight based on 100 parts by weight of the dried gardenia,
The bamboo leaf extract,
It is extracted from the dried bamboo leaves of 200 parts by weight based on 100 parts by weight of the dried gardenia,
The maekmundong extract,
It is extracted from the dried maekmundong of 50 parts by weight based on 100 parts by weight of the dried gardenia,
The content of the plant complex extract is 5% by weight of the anti-inflammatory cosmetic composition. The method according to claim 1,
The extract is an anti-inflammatory cosmetic composition that is extracted with an extraction solvent selected from the group consisting of water, an organic solvent, and a mixed solvent thereof. 3. The method according to claim 2,
The organic solvent is one selected from the group consisting of alcohols having 1 to 4 carbon atoms, ethyl acetate, glycerol, ethylene glycol, propylene glycol, butylene glycol, and combinations thereof. delete delete delete delete The method according to claim 1,
A cosmetic composition for anti-inflammatory that further comprises water. The method according to claim 1,
A cosmetic composition for anti-inflammatory that further comprises a cosmetic base composition. In the method for preparing the anti-inflammatory cosmetic composition of claim 1,
Angelicasinensis ), peppermint ( Mentha arvensis var. piperascens ), upper leaves ( Morusalba leaves ), bamboo leaves ( Sasa borealis ), gardenia ( Gardeniae Fructus ) and maekmundong each dried product is mixed with an extraction solvent to prepare a respective mixture;
heating each mixture to extract each extract;
preparing a plant complex extract by mixing the respective extracts; and
Mixing the plant complex extract with a cosmetic base composition to prepare a cosmetic composition,
The Angelica extract is
It is extracted from 130 parts by weight of the dried angelica root with respect to 100 parts by weight of the dried gardenia,
The mint extract is
It is extracted from the dried mint of 200 parts by weight based on 100 parts by weight of the dried gardenia,
The upper leaf extract,
It is extracted from the dried upper leaves of 100 parts by weight based on 100 parts by weight of the dried gardenia,
The bamboo leaf extract,
It is extracted from the dried bamboo leaves of 200 parts by weight based on 100 parts by weight of the dried gardenia,
The maekmundong extract,
It is extracted from the dried maekmundong of 50 parts by weight based on 100 parts by weight of the dried gardenia,
The method for producing a cosmetic composition for anti-inflammatory that the content of the plant complex extract is 5% by weight. 11. The method of claim 10,
The temperature of the heating step is 40 ℃ or more and 80 ℃ or less of the method of manufacturing an anti-inflammatory cosmetic composition. 11. The method of claim 10,
The heating step is a method for producing a cosmetic composition for anti-inflammatory that is to apply an ultrasonic wave of 700 kHz or more and 1,200 kHz or less.

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