KR20190086317A - A composition for prevention or improvement of atopic dermatitis containing a herbal complex extract - Google Patents

Abstract

The present invention relates to: a cosmetic composition for preventing or improving atopic dermatitis, which contains composite extracts of green tea leaves, houttuynia, gardenia, acanthopanax, mints, quince, cornus officinalis, Chinese matrimony vines, angelica, Sophora flavescens Aiton, elm roots, white chrysanthemum, yellow chrysanthemum, mulberries, burdocks, scutellaria, turmeric, gingers, licorice, and schizandra chinensis; a cosmetic composition for antibacterial, anti-inflammatory or antioxidation for skin containing the composite extract; a food composition for preventing or improving atopic dermatitis; a quasi-drug composition for preventing or improving atopic dermatitis; a food composition for antibacterial, anti-inflammatory or antioxidation for skin; and a quasi-drug composition for antibacterial, anti-inflammatory or antioxidation for skin. The present invention confirms that the composite extract is antioxidant and anti-inflammatory and has an excellent antibacterial activity against Staphylococcus aureus. Accordingly, the composition comprising the composite extract may be used as a functional cosmetic product for skin moisturization, antiaging and improvement of atopic skin and for preventing or alleviating a secondary infection of atopic dermatitis.

Description

TECHNICAL FIELD The present invention relates to a composition for prevention or improvement of atopic dermatitis,

The present invention relates to a composition comprising a green tea leaf, a green tea plant, a gardenia, an acacia, a peppermint, a coriander, a coriander oil, a Gugija, a Angelica gigas, a ginseng root, an elm roots, A cosmetic composition for preventing or improving atopic dermatitis; A cosmetic composition for skin antibiosis, anti-inflammation or antioxidation comprising the above-mentioned complex extract; A food composition for preventing or improving atopic dermatitis; A quasi-drug composition for preventing or improving atopic dermatitis; Food composition for skin antimicrobial, anti-inflammatory or antioxidant; And a quasi-drug composition for skin antibiosis, anti-inflammation or antioxidation.

Atopy is derived from ancient Greek 'atopia', meaning 'strange, abnormal', and refers to an allergic reaction in which the body becomes extremely sensitive without medically contacting allergens. Atopic symptoms can appear in different parts of the body. When atopic symptoms appear as skin symptoms, it is called atopic dermatitis. Atopic dermatitis is a chronic inflammatory skin disease that mainly causes itching and skin lesions. In severe cases, secondary infection by bacteria may cause symptoms such as blistering or scabs. Atopic dermatitis occurs mainly during infancy, but it is often persisted to adolescents and adults. According to a survey by the CDC, the number of patients with atopic dermatitis, which was 4% in 1995, increased by 3.2% to 13% in 2010, and in most countries, 10% of children and 1% of adults have prevalence rates (Larsen and Hanifin, 2002). The causes of these atopic dermatitis include environmental factors, genetic predisposition, or immune system abnormalities, but the major causes are not well known.

Since the underlying cause of the disease has not been identified, efforts to prevent long-term steady-state treatment and recurrence are required. However, currently, the method of preventing or treating atopic dermatitis is limited to treating steroids, immunomodulators, antihistamines and the like, or reducing the irritation of the skin, maintaining moisturization and improving lifestyle to improve immunity have. However, drugs such as steroids and antihistamines are limited in their use due to their side effects. In particular, steroid drugs most commonly used for the treatment of atopic dermatitis are known to have side effects such as tolerance and abnormal endocrine system. On the other hand, recently, gamma-linoleic acid, which is widely used for atopic dermatitis, is easily oxidized and not only low in stability but also has a strong skin irritation, making it difficult to use it for sensitive skin. In addition, improvement of lifestyle alone still limits the improvement of atopic dermatitis.

Accordingly, there is a desperate need to develop a composition for preventing or ameliorating atopic dermatitis produced using natural materials and a product utilizing the same, which are not accompanied by such problems or side effects. In this regard, The interest in functional cosmetics manufactured using the cosmetics has increased. Accordingly, a composition for improving atopic dermatitis (Korean Patent Laid-Open Publication No. 2016-0019190) containing ginsenoside Rd enhanced fermented ginseng or red ginseng extract, an atopic skin cosmetic composition containing at least two kinds of extracts, Have been developed.

On the other hand, medicinal plants used in oriental medicine such as green tea leaves, oriental herb, gardenia, ogapi, mint, mosquito, coriander, gugija, angelica, gosam, elm roots, white kingdom, It is known that there are effects such as improvement of immunity, acceleration of metabolism, detoxification, promotion of digestion, protection of liver function and the like. However, the above-mentioned combination was not used as a cosmetic composition, and the effect of the above-mentioned combination on atopic dermatitis was not known.

Under these circumstances, the present inventors have made extensive efforts to develop a composition for preventing or ameliorating atopic dermatitis using a natural-derived herbal material. As a result, the present inventors have found that a composition for preventing or ameliorating atopic dermatitis, , And elm roots, white kingdom, kingdom, odi, burdock, golden, turmeric, ginger, licorice and omija have excellent antimicrobial activity against antioxidant, anti-inflammatory and Staphylococcus aureus. Further, And atopic dermatitis, and to prevent or ameliorate secondary infection of atopic dermatitis. The present invention has been completed based on this finding.

One object of the present invention is to provide a green tea leaf, a green tea plant, a gardenia, an oak, a peppermint, a coriander, a coriander oil, a gujie, a ginseng, a ginseng, an elm roots, And to provide a cosmetic composition for preventing or improving atopic dermatitis.

Another object of the present invention is to provide a cosmetic composition for skin antibacterial, anti-inflammation or antioxidation comprising the above-mentioned complex extract.

It is still another object of the present invention to provide a food composition for preventing or improving atopic dermatitis, which comprises the above-mentioned complex extract.

It is still another object of the present invention to provide a quasi-drug composition for preventing or improving atopic dermatitis, which comprises the above-mentioned complex extract.

It is another object of the present invention to provide a skin-antibacterial, anti-inflammatory or antioxidant food composition containing the above-mentioned complex extract.

It is still another object of the present invention to provide a quasi-drug composition for skin antimicrobial, anti-inflammation or antioxidation comprising the above-mentioned complex extract.

This will be described in detail as follows. On the other hand, each description and embodiment disclosed in the present application can be applied to each other description and embodiment. That is, all combinations of the various elements disclosed in this application fall within the scope of the present application. Further, the scope of the present application is not limited by the specific description described below.

In accordance with one aspect of the present invention, there is provided a method for preparing a green tea leaf, a green tea plant, a gardenia, an oak, a peppermint, a coriander, a coriander, The present invention provides a cosmetic composition for prevention or improvement of atopic dermatitis comprising ginger, licorice and Omija complex extract.

In the present invention, a combination of green tea leaves, oriental herb, gardenia, ogapi, mint, mosquito, coriander, gugija, angelica, ginseng, elm roots, white kingdom, , Anti-inflammatory activity was confirmed, and in particular, the synergistic antimicrobial activity against Staphylococcus aureus of the complex extract was confirmed in comparison with the weight of each herbal material, thereby preventing or improving the secondary infection of atopic dermatitis and atopic dermatitis Can be used. It has not been previously known that the combined extract of the combination exhibits a preventive or ameliorative effect on atopic dermatitis, and it was first discovered by the present inventors.

In the present invention, 'green tea leaf' refers to a leaf of dried tea which is roasted without being fermented, and may be named in combination with green tea. Camellia sinensis is a perennial evergreen shrub belonging to Camellia sinensis . The major components of green tea are catechin, caffeine, theanin, etc., and have anti-cancer effect, prevention of hypertension and arteriosclerosis, , Prevention of obesity, and immunity enhancing effect.

In the present invention, '魚 草 草' means the upper part of the flowering period of the Hunchunia cordata Thunberg . The leaves of Hwasungcho have a characteristic odor, characterized by spicy taste and a slightly cold taste, and are known to have effects such as antipyretic, antidiuretic, vasodilating, and promoting drainage.

In the present invention, 'gardenia' is dried mature fruit of gardenia ( Gardenia jasminoides Ellis ) belonging to madder family and has distinctive smell and taste. In one room, the effects of fever, ejaculation, and hemostasis are known.

In the present invention, 'ogapi' refers to the root bark and stem bark of the Aralthopanax sessiliflorum Seeman or other alpine plants of Araliaceae, Aralia spp., With a characteristic odor, spicy and spicy, and warm in texture. Improvement of liver and kidney function, immunity enhancement, endocrine function control, detoxification, and blood pressure control have been reported.

In the present invention, 'peppermint' refers to the above-ground part of peppermint ( Mentha arvensis Linne var. Piperascens Malinvaud ex Holmes ). It is characterized in that menthol is the main component and peppermint is smelled throughout the plant. The effects of sedation, analgesia, and genomes are known.

In the present invention, 'quince' refers to fruit of the quince tree ( Chaenomeles sinensis Koebhne ), which is characterized by strong acidity, firmness and strong aroma. It promotes metabolism, promotes the secretion of digestive enzymes, improves digestive function, and is known to cause gangdam action.

In the present invention, 'mountain oil' refers to mature fruit of a dogwood tree and Cornus officinalis Siebold et Zuccarini , which is slightly sour and sweet with a strong acidity. The pulp contains glycosides such as cornin, morroniside, loganin and tannin, and organic acids such as wine and malic acid. In one room, it is known to be used as a medicinal product for tonic, dizziness, fever, tinnitus, and menstrual overgrowth.

In the present invention, 'Gugija' is a fruit of Lycium chinense Miller belonging to the branch family, which is initially sweet and has a bitter taste, and betaine, zeazanthin, carotene ), And it is known that it inhibits the accumulation of fat in the liver, decreases blood pressure, decreases visual acuity, and strengthens immunity.

In the present invention, ' Angelica gigas Nakai ' refers to the roots of Angelica gigas Nakai , which is characterized by its warm, spicy and spicy characteristics. The main effect of angelica is to produce blood, which is known to be a blood-producing effect. In addition, it promotes erythropoiesis, sedation, analgesia, hypotension, and dysfunction.

In the present invention, 'gamseng' means dried roots or roots of Sophora flavescens Solander ex Aiton with rosewood beans. There is a peculiar smell, and the weakness is very heavy and cold. In one room, it is known to prescribe to indigestion, neuralgia, jaundice.

In the present invention, the term "elm roots or 根 肌" refers to a root of an elm ( Ulmus davidiana var. Japonica ), a deciduous broad-leaved tree belonging to the nettle family Neeta , and is tasteless, Feature. When used as a medicinal product, it has an effect of helping excretion, and is effective for gastrointestinal diseases and insomnia.

In the present invention, 'white chrysanthemum' means dried flower of Chrysanthemum morifolium Ramatuelle . It has a heat- reducing effect and is used for protection of liver function, hypertension, congestion and physiological impurity.

In the present invention, 'Huangchu' means dried flower of Chrysanthemum indicum L. or Chrysanthemum morifolium Ramatuelle , and can be named as a true country or a country. There is a distinctive scent, a little feather is characterized by a little sweet and cold. It has the effect of lowering heat, and it is used for protection of liver function, hypertension, congestion, menstrual irregularity.

In the present invention, "audi" means a fruit of Morus or a medicament which is dried with it, and is also referred to as loss, heartache, or sickness. Weakness is mild, taste is sweet. Diuretic action, shrinking, tonic action, facilitating bowel movements, and the ability to detoxify alcohol poisoning is known.

"Burdock (牛蒡)" in the present invention can mean that the base of the plant belonging to the genus Compositae Burdock (Arctium), specifically burdock (Arctium lappa Linne), but is not limited thereto. It is known that it can decrypt toxins of the liver, purify blood, and exert diuretic action and digestion promoting action.

In the present invention, '黄 芩' means the removal of the roots or junks of the spiny grass ( Scutellaria baicalensis Georgi ). It is characterized by little odor and slightly bitter taste, and it is known that it has effects such as fever, diuretic, branch, and ejaculation.

In the present invention, '薑黄' means rootstocks of ginger ginger and Curcuma longa Linne . The taste is spicy and the quality is very warm. In one room, it is known that it promotes blood circulation, eliminates eosinophilia, alleviates pain, and improves menstruation.

In the present invention, 'ginger' refers to the roots of the ginger root ( Zingiber officinale Roscoe ) with ginger ginger. It is characterized by its light yellowish spots and fragrance. It is used because it has the effect of metabolic function, digestion deficiency, sore throat, diarrhea, vomiting and so on.

In the present invention, 'licorice' means the root and rootstock of licorice ( Glycyrrhiza uralensis Fischer ), European licorice ( Glycyrrhiza glabra L. ) or other inbred plants. It has a peculiar smell and taste is sweet. The pharmacological action is known to be detoxification, shrinkage, germane, diuretic action and suppression of peptic ulcer.

In the present invention, " Omiza " means mature fruit of Schizandra chinensis Baillon tree of Omiza. Major active ingredients include lignan, essential oil, etc. Liver protection, blood pressure reduction, immunity enhancement and shrinkage, and gadamer efficacy are known.

In the present invention, the 'extract' refers to a green tea leaf, a green tea plant, a gardenia, an oak, a peppermint, a coriander, a coriander oil, a gugija, a ginseng, a ginseng, an elm roots, Extracts of all the formulations which can be formed using the extract itself and the extract, such as an extract obtained by extracting the extract, a diluted or concentrated liquid of the extract, a dried product obtained by drying the extract, a preparation or a purified product of the extract, But are not limited thereto.

The term 'complex extract' in the present invention refers to an extract obtained by extracting a mixture containing at least two of the above-mentioned 20 herbal materials. Specifically, the extract may be a mixture of 20 kinds of herbal materials And can be used in combination with 'herbal composition extract' in the present invention.

The method for extracting the herbal composition extract of the present invention is not particularly limited and may be carried out according to a method commonly used in the art. Non-limiting examples of the extraction method include hydrothermal extraction, ultrasonic extraction, filtration, and reflux extraction. These may be performed alone or in combination with two or more methods.

In the present invention, the extraction solvent used for extracting the herbal composition extract may be any solvent known in the art. Preferably, the extraction solvent may be a mixture of water and butyleneglycol for daily mixing. Specifically, the mixed solvent may have a mixing ratio of water to butyleneglycol of 1: 1.

In the present invention, the complex extract of Comparative Example 1 using purified water as an extraction solvent had a specific odor and an opaque precipitate of yellowish brown color. However, the complex extract of Example 1 using a mixed solvent of water and butylene glycol showed a dark It was confirmed to be a clear yellowish brown liquid form. From the viewpoint of contamination, the complex extract of Comparative Example 1 was found to be easily contaminated with bacteria such as mold when left at room temperature for 1 to 2 days, while the compound extract of Example 1 showed no contamination even when left at room temperature for 30 days or more Respectively. Therefore, it is suggested that the herbal composition extract of Example 1, which is extracted using a mixed solvent of water and butyleneglycol, has better storage stability than the sensory part when applied to cosmetic compositions and cosmetics.

In addition, it was confirmed that the compound extract of Example 1 had remarkably excellent antimicrobial and antioxidative activity at a relatively low concentration compared to the compound extract of Comparative Example 1. This means that the extract of Example 1, which was extracted using a mixed solvent of water and butyleneglycol, Can be usefully used as a cosmetic composition for improving atopic dermatitis, antimicrobial, antioxidant and anti-inflammatory.

In the present invention, the oriental herb complex extract may comprise 20 to 80% by weight of purified water, 20 to 80% by weight of butylene glycol, 0.1 to 0.5% by weight of green tea leaves, 0.1 to 0.5% 0.01 to 0.1% by weight of a gardenia, 0.1 to 0.5% by weight of an apple, 0.05 to 0.25% by weight of a peppermint, 0.1 to 0.1% by weight of corn, 0.2 to 1.0% by weight of corn oil, 0.2 to 1.0% 0.05 to 0.5% by weight of cordierite, 0.1 to 0.5% by weight of odor, 0.1 to 0.5% by weight of burdock, 0.1 to 0.5% by weight of gold, 0.5 to 2% by weight of ginseng, 1 to 10% , 0.01 to 0.1% by weight of sulfur, 0.01 to 0.1% by weight of ginger, 0.1 to 0.5% by weight of licorice and 1 to 10% by weight of omelet. The specific content ratio of the herbal composition extract is shown in Table 1 below.

In the present invention, 'atopic dermatitis' refers to a chronic inflammatory skin disease that mainly causes itching and skin lesions. Further, it may cause symptoms such as blistering or scabbing due to secondary infection caused by bacteria Inflammatory skin disease. Although both environmental and genetic factors are known to be involved in the onset of atopic dermatitis, the exact cause of the disease has not been elucidated. Specifically, the bacterium may be, but not limited to, Staphylococcus aureus.

In the present invention, ' Staphylococcus aureus ' is a gram-positive bacterium (pseudomonas) having a grape cluster shape and a diameter of 0.8-1.0 μm, and is a bacterium existing in nasal mucosa and skin of human and animal. Staphylococcus aureus is present in the skin, but the skin of patients with atopic dermatitis breaks the balance of supernatant bacteria, the number of which rapidly increases, colonies form secondary infection and aggravation of atopic dermatitis to be. Therefore, when the Staphylococcus aureus is killed in the skin of the atopic dermatitis patient, the symptoms due to atopic dermatitis and secondary infection, such as reduction of itch and decrease of blisters and lesions, can be improved. In one embodiment of the present invention, the herb complex extract of the present invention showed remarkably excellent antibacterial activity as compared with the control group, gentamicin, and it was confirmed that the herb extract has higher synergistic antimicrobial activity than the herbal extract. This suggests that the herbal composition extract of the present invention can be usefully used for the prevention or improvement of atopic dermatitis and secondary infections thereof.

&Quot; Prevention " in the present invention means all actions for inhibiting or delaying atopic dermatitis by administration of the composition comprising the herbal composition extract.

In the present invention, 'improvement' means that the administration of the composition containing the herbal composition extract reduces the degree of atopic dermatitis symptoms, reduces the itching, the generation of skin lesions, or alleviates symptoms due to secondary infection of atopic dermatitis It means all the acts that you do.

The components included in the cosmetic composition of the present invention include components commonly used in cosmetic compositions, such as water, a surfactant, a moisturizer, a lower alcohol, a chelating agent, a bactericide, an antioxidant, , Coloring matters, and flavoring agents.

In addition, the cosmetic composition may be prepared in any conventional formulation and may be in the form of solutions, emulsions, suspensions, pastes, creams, lotions, gels, powders, sprays, surfactant-containing cleansing, oils, But are not limited to, liquid cleansing agents, bath salts, foundation, makeup base, essence, lotion, foam, pack, suppositories, sunscreen cream or sun oil.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, or tracant may be used.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters. In addition, the ingredients contained in the cosmetic composition may be included in an amount commonly used in the skin science field.

Another aspect of the present invention to attain the above object is to provide a cosmetic composition for skin antibiosis, anti-inflammation or antioxidation comprising the herbal composition extract. The herbal composition extract and the cosmetic composition are as described above.

In the present invention, 'antimicrobial' is an effect of inhibiting the growth or proliferation of microorganisms, specifically atopic dermatitis-related bacteria. More specifically, it means an effect of inhibiting the growth or proliferation of pathogenic microorganisms causing secondary infection of atopic dermatitis. But is not limited thereto.

In the present invention, the herbal composition extract may have antimicrobial activity against Staphylococcus aureus which is a causative agent of secondary infection of atopic dermatitis. In one embodiment of the present invention, the antimicrobial activity of each herbal extract and the herbal composition extract of Staphylococcus aureus used in the present invention was evaluated. As a result, it was found that the herbal composition extract showed synergistic antimicrobial activity and the antimicrobial activity was remarkably higher than that of the control group gentamycin. Respectively. This suggests that the herbal composition extract may be useful for preventing or improving secondary infection of atopic dermatitis and atopic dermatitis.

In the present invention, 'antioxidation' refers to inhibition of cellular oxidation by free radicals or reactive oxygen species (ROS), which are highly reactive depending on the oxidative stress caused by intracellular metabolism or ultraviolet rays Quot; refers to the removal of free radicals or reactive oxygen species, thereby reducing cellular damage. In one specific embodiment of the present invention, the antioxidant activity of the herbal composition extract of the present invention and ascorbic acid as a positive control group was evaluated using DPPH assay, and as a result, it was found that the herbal composition extract had the same or lower concentration than ascorbic acid It was confirmed that there is a similar level of antioxidant activity. This suggests that the herbal composition extract may be useful as a cosmetic composition for skin antioxidation.

In the present invention, " anti-inflammation " means all actions that inhibit the onset or deterioration of inflammation. The inflammation is one of defensive reactions of biological tissue against certain stimuli, and involves three kinds of tissue degeneration, circulatory disorder, Of the lesions. The anti-inflammatory activity can be confirmed by using Nitric oxide (NO), which is a powerful inflammatory mediator, as an indicator. In one specific example of the present invention, the ability of the herbal composition extract to inhibit nitric oxide (NO) production was evaluated. As a result, it was confirmed that the herbal composition extract showed excellent NO production inhibitory effect even at a low concentration of 0.5 to 1% . This suggests that the herbal composition extract may be useful as a cosmetic composition for skin anti-inflammation.

Another aspect of the present invention to attain the above object is to provide a food composition for prevention or improvement of atopic dermatitis comprising the herbal composition extract. The herbal composition extract and atopic dermatitis are as described above.

In the present invention, the term 'food' refers to a food such as meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, ice cream, dairy products, various soups, beverages, tea, A health food, and a health food, all of which include foods in a conventional sense.

The term "functional food" as used herein means the same term as "food for special health use" (FoSHU). In addition to nutrition, It means food. Here, 'function (surname)' refers to the structure and function of the human body to obtain a beneficial effect for health use such as controlling nutrients or physiological action. The food of the present invention can be prepared by a method commonly used in the art and can be prepared by adding raw materials and ingredients which are conventionally added in the art. In addition, the above-mentioned food formulations can be manufactured without limitations as long as they are formulations recognized as food. The composition for food of the present invention can be manufactured in various formulations, and unlike ordinary drugs, it has advantages of being free from side effects that may occur when a natural product is used as a raw material for a long period of time, and is excellent in portability. The food of the invention can be ingested as an adjuvant to promote the improvement effect of atopic dermatitis.

The health food refers to a food having an active health promotion or promotion effect compared with a general food, and a health supplement food refers to a food for health assistance. In some cases, the terms health functional foods, health foods, and health supplements are mixed.

Specifically, the health functional food is a food prepared by adding the compound of the present invention to a food material such as a beverage, a tea, a spice, a gum, confectionery, or the like, or encapsulated, powdered or suspended therein. But it has the advantage that there is no side effect that can occur when the food is used as a raw material and long-term use of the drug.

The food composition may further comprise a physiologically acceptable carrier. The type of carrier is not particularly limited, and any carrier conventionally used in the art can be used.

In addition, the food composition may contain additional components that are commonly used in food compositions and can improve odor, taste, visual appearance, and the like. For example, vitamins A, C, D, E, B1, B2, B6, B12, niacin, biotin, folate, panthotenic acid and the like. Minerals such as zinc (Zn), iron (Fe), calcium (Ca), chromium (Cr), magnesium (Mg), manganese (Mn), copper (Cu) and chromium (Cr); And amino acids such as lysine, tryptophan, cysteine, valine, and the like.

In addition, the food composition may further contain antiseptic agents (such as potassium sorbate, sodium benzoate, salicylic acid, sodium dehydroacetate), bactericides (Sodium nitrite), bleach (sodium sulfite), seasoning (sodium MSG glutamate, etc.), sweeteners (dicin, cyclamate, saccharin, etc.), coloring agents , Sodium, etc.), perfume (vanillin, lactones, etc.), swelling agents (alum, potassium hydrogen D-tartrate), emulsifiers, thickeners (foams), encapsulating agents, gum bases, foam inhibitors, solvents, And may include food additives. The additives may be selected and used in appropriate amounts depending on the type of food.

As an example of the food composition of the present invention, it can be used as a health beverage composition. In this case, various flavors or natural carbohydrates can be added as an additional ingredient like ordinary beverages. The above-mentioned natural carbohydrates include monosaccharides such as glucose and fructose; Disaccharides such as maltose, sucrose; Polysaccharides such as dextrin, cyclodextrin; Xylitol, sorbitol, erythritol, and the like. Sweeteners include natural sweeteners such as tau Martin and stevia extract; Synthetic sweetening agents such as saccharin and aspartame, and the like can be used. The ratio of the natural carbohydrate may be generally about 0.01 to 0.04 g, specifically about 0.02 to 0.03 g per 100 ml of the health beverage composition of the present invention.

In addition to the above, the health beverage composition may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid, salts of pectic acid, alginic acid, salts of alginic acid, organic acid, protective colloid thickener, pH adjuster, stabilizer, Alcohols or carbonating agents, and the like. It may also contain flesh for the production of natural fruit juices, fruit juice drinks, or vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not critical, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the health beverage composition of the present invention.

Another aspect of the present invention to attain the above object is to provide a quasi-drug composition for prevention or improvement of atopic dermatitis comprising the herbal composition extract.

In the present invention, the term 'quasi-drug' means a fiber, a rubber product or the like used for the purpose of treating, alleviating, treating or preventing a disease of a person or an animal, And similar products, products for use in sterilization, insecticide and similar uses for the prevention of infection, products for the purpose of diagnosis, treatment, alleviation, treatment or prevention of diseases of humans or animals, Machinery, or apparatus, and that is not an apparatus, machine, or apparatus of an article used for the purpose of imparting pharmacological effects to the structure or function of a person or animal. .

When the herbal composition complex extract of the present invention is added to a quasi-drug composition for the purpose of prevention or improvement of atopy, the complex extract may be added as it is or may be used together with other quasi-drugs, and may be suitably used according to a conventional method. The amount of the active ingredient to be mixed can be suitably determined according to the intended use (prevention, health or therapeutic treatment).

The quasi-drug composition includes, but is not limited to, personal hygiene products, external preparation for skin, disinfectant cleaner, shower foam, wet tissue, detergent soap, hand wash, mask or ointment. The external preparation for skin is not particularly limited, but may be manufactured and used in the form of an ointment, a lotion, a spray, a patch, a cream, a powder, a suspension, a gel or a gel. The personal hygiene product may be, for example, a soap, a wet tissue, a tissue paper, a shampoo, a toothpaste, a hair care product, an air freshner gel, or a cleaning gel.

Another aspect of the present invention to attain the above object is a food composition for skin antibiosis, anti-inflammation or antioxidation comprising the herbal composition extract; And a quasi-drug composition. The herbal composition extract, antibacterial, anti-inflammatory and antioxidant are as described above.

According to another aspect of the present invention, there is provided a pharmaceutical composition for preventing or treating atopic dermatitis, which comprises the herbal composition extract. The herbal composition extract and atopic dermatitis are as described above.

The pharmaceutical composition may further comprise a pharmaceutically acceptable carrier, excipient or diluent conventionally used in the manufacture of a pharmaceutical composition, and the carrier may comprise a non-naturally occuring carrier . Examples of the carrier, excipient and diluent include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, Cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil.

The pharmaceutical compositions may be formulated into tablets, pills, powders, granules, capsules, suspensions, solutions, emulsions, syrups, sterilized aqueous solutions, nonaqueous solutions, suspensions, emulsions, It can be formulated in the form of a percutaneous absorbent, a gel, a lotion, an ointment, a cream, a patch, a cataplasma, a paste, a spray, a skin emulsion, a skin suspension, a transdermal patch, a drug containing bandage or suppository .

The present invention relates to an antioxidant, antioxidant, antioxidant, antioxidant, antioxidant, antioxidant, antioxidant, antioxidant, antioxidant, antioxidant, antioxidant, antioxidant, antioxidant, antioxidant, antioxidant, antioxidant, It has been confirmed that the cosmetic composition comprising the complex extract has an excellent antimicrobial activity against anti-inflammation and Staphylococcus aureus. Therefore, the cosmetic composition comprising the complex extract can prevent or ameliorate skin moisturizing, anti-aging and atopic skin and secondary infection of atopic dermatitis Which can be used as a functional cosmetic for the purpose of

1 shows a process for preparing the herbal composition extract of the present invention.
Fig. 2 compares the properties of the herbal composition extract of Example 1 and Comparative Example 1. Fig.
Fig. 3 shows the antimicrobial activity against Staphylococcus aureus in the group of 200 占 퐇 of the herbal composition extract of Comparative Example 1. Fig.
Fig. 4 shows the results of confirming the antimicrobial activity against Staphylococcus aureus KACC10768 of each herbal ingredient and the herbal composition extract contained in the herbal composition extract of Example 1. Fig.
Specifically, C-gentamycin (10 mg / ml) in Figs. 4A to D; 1-green tea leaf extract; 2-thymus extract; 3 - Gardenia extract; 4- Ogaki extract; 5-mint extract; 6-Mosaic extract; 7-Corn oil extract; 8-Gujee Extract; 9 - Angelica gigantosa extract; 10-Gossam Extract; 11-elm roots extract; 12-white rot extract; 13- Chrysanthemum morifolium extract; 14-odi extract; 15-Burdock extract; 16-Golden extract; 17-turmeric extract; 18-ginger extract; 19-Licorice extract; 20-Omija extract.
Also, in Fig. 4E, C-gentamycin (10 mg / ml); 50 - 50% of herbal complex extract, 50 - 50% of herbal complex extract, 50 - 50% of herbal complex extract, 50 - 50 < / RTI >
Figure 5 The antioxidant activity of the herbal composition extract of Comparative Example 1 was shown by concentration.
6 shows the antioxidative activity of the herbal composition extract according to the concentration of Example 1. FIG.
Figure 7 shows the antioxidant activity of ascorbic acid as a positive control group.
8 shows the NO production inhibition rate of the herbal composition extract of Example 1 by concentration.
FIG. 9 shows the inhibition of NO production by the concentration of L-NMMA, which is a positive control group.
FIG. 10 shows the cell survival rate of human dermal fibroblasts according to the concentration of the herbal composition extract of Example 1. FIG.

Hereinafter, the constitution and effects of the present invention will be described in more detail with reference to Examples and Experimental Examples. These Examples and Experimental Examples are only for illustrating the present invention, but the scope of the present invention is not limited thereto.

Example 1: Preparation of a complex extract using a mixed solvent of purified water and butyleneglycol as an extraction solvent

20 kinds of domestic products made of green tea, ginger, gardenia, ginger, mint, mosquito, coriander, gugija, angelica, gossam, elm roots, white kingdom, oriental kingdom, burdock, golden turmeric, ginger, licorice, The herbal extracts were prepared from oriental herbs. The process for preparing the herbal composition extract was largely divided into washing, extraction and filtration steps. First, each herbal material is washed three times with flowing water, rinsed once with purified water, and then completely dried at 30 to 40 ° C for 2 to 3 hours. Thereafter, the weight of each dried herbal material is weighed, mixed according to the content ratio, and extracted at 80 to 90 ° C for 2 to 3 hours using a mixed solvent of 1: 1 of purified water and butyleneglycol . The content ratio of the extraction solvent and 20 kinds of herbal materials is shown in Table 1 below.

[Table 1]

Then, the obtained extract was cooled at 30 to 40 占 폚. Finally, the thus-cooled extract was recovered using a nonwoven fabric, and the recovered extract was firstly filtered using a housing filter (25 mu m) and then subjected to secondary filtration using a housing filter (1 mu m) The preparation of oriental herb complex extract was completed. The overall process for preparing the herbal composition extract is shown in Fig.

Comparative Example 1: Preparation of a complex extract using purified water as an extraction solvent and a property comparison with the complex extract of Example 1

The properties of the herbal composition extract (hereinafter, referred to as "Comparative Example 1") and the herbal composition extract of Example 1 were compared using the same 20 kinds of herbal materials as in Example 1, but using only purified water as an extraction solvent. The specific content ratios of the purified water and the herbal materials contained in Comparative Example 1 are shown in Table 2 below.

[Table 2]

The manufacturing process of the herbal composition extract of Comparative Example 1 was largely divided into washing, extraction and filtration steps. First, each herbal material was washed twice with running water, and rinsed once with purified water to remove water. Then, 20 kinds of herbal materials were added to the extraction tank containing purified water, extracted at 70 ± 5 for 12 hours, and further extracted at room temperature for 36 hours. The resultant complex extract was recovered using a nonwoven fabric, and the recovered complex extract was filtered using a housing filter (1 mu m) to complete the preparation.

As shown in FIG. 2, the complex extract of Comparative Example 1 had a specific odor and an opaque precipitate of yellowish brown color was present. However, the complex extract of Example 1 The extract was found to be a deep yellowish brown clear liquid form. From the viewpoint of contamination, the compound extract of Comparative Example 1 was found to be easily contaminated with bacteria such as mold when left at room temperature for 1 to 2 days, while the compound extract of Example 1 was maintained without contamination even when left at room temperature for 30 days or longer Respectively.

This indicates that the herbal composition extract of Example 1 is superior to the cosmetic composition and cosmetic composition of Comparative Example 1 in the sensory part and is also excellent in long-term storage property.

Experimental Example 1: Staphylococcus aureus of a herbal composition extract Staphylococcus aureus ) To evaluate the antimicrobial activity against

Experimental Example 1-1: Evaluation of antibacterial activity against Staphylococcus aureus of the herbal composition extract of Comparative Example 1

The antimicrobial activity against Staphylococcus aureus KACC10768, a pathogenic strain causing secondary infection of atopic dermatitis of the herbal composition extract prepared in Comparative Example 1, was evaluated according to the liquid culture method. 100 [mu] l of the above strain was inoculated into a liquid medium of soybean casein digest agar (15.0 g of casein peptone, 5.0 g of soybean peptone, and 5.0 g of sodium chloride), and the complex extract of Comparative Example 1 was inoculated at 0, 50, 100, 150, and 200 μl each were incubated for 48 hours with shaking at 37 ° C. The culture was incubated with UV-vis spectrophotometer to measure the absorbance at 660 nm. As a result, as shown in FIG. 3, about 50% of the antimicrobial activity was confirmed in the extract-treated medium at a concentration of 200 .mu.l compared to the medium not treated with the extract.

Experimental Example 1-2: Evaluation of Antimicrobial Activity against Staphylococcus aureus of the Oriental Composite Extract of Example 1

In order to evaluate the antimicrobial activity against Staphylococcus aureus KACC10768, a pathogenic strain responsible for secondary infection of atopic dermatitis of the herbal composition extract prepared in Example 1, gentamicin was used as a control group, The paper disc method was used. Each herbal composition was added according to the contents of Table 1 to prepare an extract according to the process of Example 1. The herbal composition extract containing all of the 20 kinds of herbal materials was prepared in the same manner as in Example 1 ≪ / RTI >

For the quantitative evaluation of the antimicrobial activity, on the soybean casein digest agar culture medium (0.7% of Tryptic soy agar, 15.0 g of casein peptone, 5.0 g of soybean peptone, 5.0 g of sodium chloride, 975 g of purified water, pH 7.2 ± 0.1) 50 μl of each herbal extract and 20%, 40%, 60%, and 80% of herbal extracts were diluted to 100 μl of Staphylococcus aureus strain (A600, OD 1.0) And 50 ㎕ each of the stock solution. Thereafter, the cells were cultured at 37 ° C for 48 hours, and the antibacterial activity was confirmed by converting the diameter of the antimicrobial activity range in which the strain failed to proliferate into units (the diameter of the antimicrobial activity range of gentamycin 10 mg / ml was defined as one unit) Respectively.

As a result, as can be seen from FIGS. 4A to E and Table 3 below, the diameter of the antimicrobial activity range of gentamicin, which is a control group, was 12 mm, which was defined as 1 unit. As a result, Showed no significant antimicrobial activity, but only 0.66 units of antimicrobial activity was observed only in acidified milk and ginseng extract.

On the other hand, in the case of the herbal composition extract of the present invention, 1.66 units in 20% extract, 1.75 units in 40% extract, 1.75 units in 60% extract, 1.75 units in 80% And the antimicrobial activity was remarkably superior to that of the herbal extracts. In addition, since it exhibits remarkably excellent antibacterial activity at a lower concentration than the compound extract of Comparative Example 1, the herbal composition extract of the present invention can be effectively used for the prevention or improvement of atopic dermatitis and secondary infection thereof Respectively.

[Table 3]

Experimental Example 2: Evaluation of antioxidative activity of the herbal composition extract

Experimental Example 2-1: Evaluation of antioxidative activity of the herb complex extract of Comparative Example 1

In order to evaluate the antioxidative activity of the herbal composition extract of Comparative Example 1, the antioxidative activity of the complex extract was measured by the concentration of vitamin C 1% aqueous solution as a control group and the degree of oxidation inhibition of pyrogallol. Specifically, 10 ul of the complex extract of Comparative Example 1, 150 μl of Tris-HCl (pH 8.5) buffer solution and 10 μl of 7.2 mM pyrogallol were added to a test tube, followed by reaction at 25 ° C. for 10 minutes. Then, 50 μl of 1 M HCl was added to stop the reaction. After the reaction, the absorbance was measured at 420 nm using an ELISA reader. Tris-HCL buffer (pH 8.5) was added instead of the complex extract as a blank. The concentration of the sample (IC50) when the active inhibition rate was 50% was calculated using a program.

As a result, as can be seen from FIG. 5, the antioxidative effect was observed at 23% at 20% concentration, 30% at 60% concentration, 53% at 80% concentration and 56% Respectively.

Experimental Example 2-2: Evaluation of antioxidative activity of the herbal composition extract of Example 1

The ascorbic acid was used as a positive control, and the antioxidative ability of the herbal composition extract of Example 1 was evaluated through DPPH assay. Each sample diluted by concentration and 0.15 mM DPPH solution were shaded at room temperature, and the absorbance of the reaction solution was measured at 540 nm using a microplate reader. The antioxidant activity (%) was calculated by substituting each measured absorbance value into the following equation (1), and the results for each concentration of the sample were the mean ± standard deviation Respectively.

[Equation 1]

Untreated group: methanol + ultrapure water

Negative control: 0.15 mM DPPH solution + ultrapure water

Sample treatment group: 0.15 mM DPPH solution + sample diluted by each concentration

As a result, as shown in FIG. 6, FIG. 7, and Table 4 and Table 5, the antioxidant ability of the herbal composition extract of the present invention showed 91.83% at 1% to 92.40% at the sample concentration of 0.5% , And ascorbic acid, which is a positive control, has similar antioxidative activity at the same or lower concentration. In addition, since it exhibits remarkably superior antioxidative activity at a lower concentration than the compound extract of Comparative Example 1, it was confirmed that the herbal composition extract of the present invention can be effectively used as a cosmetic composition for skin antioxidation.

[Table 4]

[Table 5]

Experimental Example 3: Evaluation of anti-inflammatory activity of the herbal composition extract

In order to measure the anti-inflammatory activity of the Chinese herb complex extract, L-NMMA was used as a positive control to evaluate nitric oxide (NO) production inhibitory activity. Subcultured RAW 264.7 cells were dispensed into 96-well plates and cultured for 24 hours in a 37-well incubator containing 5% carbon dioxide, after which the culture medium was replaced with serum free media. Then, each sample diluted by concentration and LPS were treated with cells, cultured for 24 hours, and the absorbance at 540 nm was measured by reacting the cell culture medium with a grease reagent. The experiment was repeated twice, and the NO generation inhibition rate (%) was calculated by substituting each measured absorbance value into the following equation (2). The results for each concentration of the sample were the average of two repeated experiments Respectively.

&Quot; (2) "

No treatment group: No reagent treatment

Negative control: culture + LPS

Sample treatment group: Each sample diluted with LPS + culture solution

As a result, as shown in FIGS. 8, 9, 6 and 7, the herbal composition extract of the present invention showed 27.46% inhibition of NO production at a sample concentration of 0.5%, and 56.17% at 1% It was confirmed that the anti - inflammatory activity was excellent even at low concentration.

[Table 6]

[Table 7]

Experimental Example 4: Evaluation of cytotoxicity of the herbal composition extract

In order to evaluate the cytotoxicity of the herbal composition complex extract, the cell survival rate of Human Dermal Fibroblasts (HDF) treated with the complex extract was measured.

Subcultured human dermal fibroblasts were plated in 96-well plates and cultured for 24 hours in a 37-well incubator containing 5% carbon dioxide, and then the culture medium was replaced with a serum-free culture medium. Thereafter, the herbal complex extracts diluted by the respective concentrations were treated and cultured for 24 hours under cell culture conditions. In the shade condition, the MTT solution was treated in each well and allowed to react for 2 hours, and then the supernatant was removed. (formazan) was completely dissolved in DMSO and absorbance was measured at 540 nm with a microplate reader. The experiment was repeated twice, and the cell survival rate (%) was calculated by substituting each measured absorbance value into the following equation (3). The results for each concentration of the sample were the mean ± standard deviation Respectively.

&Quot; (3) "

No treatment group: No reagent treatment

Sample treatment group: Each sample diluted with culture solution

As can be seen from FIG. 10 and Table 8, the herbal composition extract of the present invention showed 97.50%, 92.94%, 0.75% to 84.78%, and 1.0% to 81.65% , And its safety was confirmed when it was used in a human body as a cosmetic composition.

[Table 8]

Experimental Example 5: Confirmation of improvement effect of atopic dermatitis in cosmetics containing herbal composition extract

Experimental Example 5-1: Preparation of lotion containing herbal composition extract

The herbal composition extract-containing lotion was prepared according to the composition shown in Table 9 below. Specifically, the herbal composite extract prepared in Example 1 and purified water were heated and mixed at 80 ° C while mixing and stirring in a usual manner, and the mixture was added to the preparation section, and phenoxyethanol; Adenosine; Xanthan gum; Sodium hyaluronate; Carbomer; Sorbitol; Aloe vera leaf juice; And disodium iodide were added thereto and dissolved, and then the emulsifying machine was operated to obtain olive oil; Tocopheryl acetate; Stres-20; Phage-75 stearate; Glyceryl stearate; Cetyl alcohol; Ceteth-20; Dimethicone; Dimethicone / vinyl dimethicone crosspolymer; Cyclopentasiloxane; Cyclohexasiloxane; Stearic acid; And palmitic acid were heated and dissolved at 80 DEG C, and then charged into the production section to emulsify. After completion of the emulsification, the mixture of the materials was cooled to 50 캜 with stirring using an agitator, and then lemon peel oil; Lavender oil; Linseed oil; And rosemary leaf oil. Finally, triethanolamine was added to adjust the pH, and the mixture was cooled to 25 ° C and aged to complete the preparation of the herbal composition extract-containing lotion.

[Table 9]

Experimental Example 5-2: Improvement of atopic dermatitis in lotion containing herbal composition extract

The clinical trial subjects were those who were at least 3 years of age with atopic skin and 70 years of age or younger who were not taking the drug at present and were divided into a test group and a control group, .

Specifically, in the test group, the lotion containing the herbal composition extract prepared in Experimental Example 5-1 was applied twice daily (morning and evening) for about 2 weeks to the dry region due to the atopic region and the atopic skin, The lotion prepared in the same manner as in Experimental Example 5-1 was used in the same manner as the test group, except that the herbal composition extract in Table 8 was excluded. During the clinical trial, the use of drugs such as atopic treatments in the test group and the control group was prohibited.

Finally, the dermatologist evaluated the severity of atopy according to the criterion shown in Table 10 below to check the degree of improvement of atopy according to the use of the lotion in the test group and the control group, The results are shown in Tables 11 and 12 below. As a result, in the control group, the pre-test average atopy severity rating was 3.5, the after-treatment atopy severity rating was 3.3, and the average improvement degree was 0.2 ± 0.40. In other words, it was confirmed that the atopic severity evaluation in the control group was hardly changed (the confidence interval of the mean m of 95% confidence interval was 0.2 ± 0.25).

On the other hand, in the test group, the pre - test average atopy severity rating was 3.3, the after - treatment atopy severity rating was 2.4, and the average improvement degree was 0.9 ± 0.54. That is, the ATOF severity evaluation in the test group was improved from moderate to moderate to mild (95% confidence interval of m mean m was 0.9 ± 0.33), so that the herbal complex extract-containing lotion was effective in prevention of atopic dermatitis Or improvement in the improvement of the performance.

[Table 10]

[Table 11]

[Table 12]

From the above description, it will be understood by those skilled in the art that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. In this regard, it should be understood that the embodiments described above are illustrative in all aspects and not restrictive. The scope of the present application is to be interpreted as being within the scope of the present application, all changes or modifications derived from the meaning and scope of the appended claims and from their equivalents rather than the detailed description.

Claims (12)

Atopic dermatitis, including green tea leaves, herringbone, gardenia, ogapi, mint, mosquito, coriander, gugija, angelica, gosam, elm roots, A cosmetic composition for prevention or improvement.
The cosmetic composition according to claim 1, wherein the complex extract is extracted with a mixed solvent of water and butyleneglycol.
The cosmetic composition according to claim 2, wherein the mixed solvent is a mixture of water and butyleneglycol in a ratio of 1: 1.
The cosmetic composition according to claim 1, wherein the atopic dermatitis comprises a second infection symptom caused by Staphylococcus aureus .
The cosmetic composition according to claim 1, wherein the complex extract has antibacterial activity against Staphylococcus aureus.
The cosmetic composition according to claim 1, wherein the cosmetic composition is selected from the group consisting of a solution, an emulsion, a suspension, a paste, a cream, a lotion, a gel, a powder, a spray, a surfactant-containing cleansing oil, Wherein the cosmetic composition has any one of the formulations selected from the group consisting of essence, lotion, foam, pack, supple water, sunscreen cream and sun oil.
Skin antimicrobial agent including green tea leaves, oriental herb, gardenia, ginger, mint, mosquito, coriander, gugija, angelica, gosam, elm roots, white bamboo, oriental, audi, burdock, golden, turmeric, ginger, licorice, , Anti-inflammatory or antioxidative cosmetic composition.
The cosmetic composition according to claim 7, wherein the cosmetic composition has antibacterial activity against Staphylococcus aureus.
Atopic dermatitis, including green tea leaves, herringbone, gardenia, ogapi, mint, mosquito, coriander, gugija, angelica, gosam, elm roots, ≪ / RTI >
Atopic dermatitis, including green tea leaves, herringbone, gardenia, ogapi, mint, mosquito, coriander, gugija, angelica, gosam, elm roots, A quasi-composition for prevention or improvement.
Skin antimicrobial agent including green tea leaves, oriental herb, gardenia, ginger, mint, mosquito, coriander, gugija, angelica, gosam, elm roots, white bamboo, oriental, audi, burdock, golden, turmeric, ginger, licorice, , Anti-inflammatory or antioxidant food composition.
Atopic dermatitis, including green tea leaves, herringbone, gardenia, ogapi, mint, mosquito, coriander, gugija, angelica, gosam, elm roots, A quasi-composition for prevention or improvement.

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