KR102294559B1 - Functional saccharide feed composition for bee - Google Patents

Abstract

The present invention relates to a bee functional sugar solution feed composition.
The bee functional sugar solution feed composition of the present invention is characterized in that it contains sugars, amino acids, and trace elements.
According to the present invention, as the expression of the antibacterial protein that enhances the physiological function of bees is increased, it has the effect of preventing the decrease in the number of bees due to climate change and the like.

Description

Bee functional saccharide feed composition {Functional saccharide feed composition for bee}

The present invention relates to a bee functional sugar solution feed composition, and more particularly, to a bee functional sugar solution feed composition that enhances the physiological function of bees.

Honey bees are one of the oldest insects to survive without evolution. These bees collect honey, pollen, royal jelly, propolis, etc. from nature and use them as a material to protect the species and the external environment. Honey bees fed by humans provide honey to humans, and humans prepare a feeding solution diluted with sugar and water 1:1 and provide it as food for bees.

However, while honey contains various components such as micronutrients and vitamins, the feed solution does not contain any nutrients other than sugar, which lowers the immunity of bees, which has a negative effect on honey production and pollen activity. is reaching

In recent years, damage such as heat waves and low temperatures due to climate change has occurred in beekeeping farms. In addition, problems such as growth disorders and resistance to honey branching occurred in the case of wheat source water, which is greatly affected by weather conditions. In addition, the bees could not collect honey due to bad weather (strong wind, low temperature, rainfall) during the flowering period of wheat raw water, so the productivity of beekeepers decreased significantly (Acacia honey produced 30% compared to the previous year).

Due to this effect, bees cannot consume natural honey with excellent nutritional value, which causes problems such as a decrease in colonies due to the occurrence of diseases.

1. Republic of Korea Patent No. 10-1311192 (Title of the invention: Method for preparing a composition for preventing and treating bee infectious diseases and nutritional composition for bees by this method, Applicant: Bioten Co., Ltd.) 2. Republic of Korea Patent Registration No. 10-1691364 (Title of the invention: Auxiliary feed composition for bees containing rice bran and method for manufacturing the same, Applicant: Iljin F&P Co., Ltd.)

An object of the present invention is to solve the problems described above, as a functional sugar solution feed composition that can improve the physiological function of bees by adding it to the existing sugar solution (sugar solution), containing sugars, various amino acids, trace elements, etc. An object of the present invention is to provide a feed composition that is effective in preventing a decrease in the number of bees due to climate change, etc. by using the composition.

The honey bee functional sugar solution feed composition of the present invention for achieving the above object is characterized in that it contains sugars, amino acids, and trace elements, and more specifically, 80 to 90 wt% of the sugar, 5 to 15 wt% of the amino acid, and It is characterized in that it is composed of 0.1 to 5% by weight of trace elements.

In addition, the saccharide is characterized in that it is composed of dextrose, maltose, trehalose, fructose, stevia sugar, and saccharin.

In addition, the amino acids are methionine, tryptophan, arginine, lysine, histidine, phenylalanine, isoleucine, threonine, leucine ) and valine.

In addition, the trace elements are characterized by being composed of calcium (Ca), potassium (K), magnesium (Mg), manganese (Mn), phosphorus (P), sodium (Na), and zinc (Zn).

In addition, the composition is malic acid, taurine, vitamin B, vitamin B2, vitamin B3, vitamin B5, vitamin B7, vitamin B8, vitamin B12, vitamin B6, vitamin C, vitamin E, caffeine (caffeine) , It is characterized in that it further comprises mint powder.

Another aspect of the present invention, bee functional sugar solution feed, is characterized in that it is composed of a mixture of sugar water and the above-mentioned honey bee functional sugar solution feed composition.

In addition, the sugar water is characterized in that the sugar is made up of 70 to 80 wt %, and the feed composition is characterized in that 10 to 20 parts by weight are mixed based on 100 parts by weight of the sugar water, and in particular, the feed is It is characterized in that it has the effect of enhancing the expression of antibacterial protein in contrast.

The technical problems to be achieved by the present invention are not limited to the technical problems mentioned above, and other technical problems not mentioned can be clearly understood by those of ordinary skill in the art to which the present invention belongs from the description below. There will be.

According to the present invention, as the expression of the antibacterial protein that enhances the physiological function of bees is increased, it has the effect of preventing the decrease in the number of bees due to climate change and the like.

1 is a graph showing the feeding preference when feeding the bee functional sugar solution feed composition of the present invention by Hamrang.
control-control
Composition 10% (Example 1- 10 parts by weight of feed composition)
Composition 20% (Example 2 - 20 parts by weight of feed composition)
Composition 30% (Comparative Example 1- 30 parts by weight of feed composition)
Composition 40% (Comparative Example 2 - 40 parts by weight of feed composition)
Composition 50% (Comparative Example 3 - 50 parts by weight of feed composition)
2 is a graph showing the expression level of antibacterial proteins expressing Apideacin and Dedensin, which are bioactive substances of bees, when feeding the bee functional sugar solution feed composition of the present invention.
con-control
Composition 10% (Example 1- 10 parts by weight of feed composition)
Composition 20% (Example 2 - 20 parts by weight of feed composition)
3 is a graph showing the expression level of the antibacterial protein expressing the bee physiologically active substances Hymenoptacin and Abaecin when the bee functional sugar solution feed composition of the present invention is fed.
control-control
Composition 10% (Example 1- 10 parts by weight of feed composition)
Composition 20% (Example 2 - 20 parts by weight of feed composition)

Hereinafter, the present invention will be described in detail, and detailed descriptions of well-known functions and configurations that may unnecessarily obscure the gist of the present invention will be omitted.

The present invention relates to a bee functional sugar solution feed composition, and more particularly, to provide a bee functional sugar solution feed composition comprising sugars, amino acids, and trace elements to enhance the expression of an antibacterial protein that enhances the physiological function of bees is characterized. More specifically, normal honey, which is the staple food of bees, contains 70 to 80% of glucose and fructose, and about 20% of moisture, sucrose, oligosaccharides, minerals, organic acids, enzymes, and the like. However, such honey is difficult to obtain in winter or when flowers are scarce, so food that can replace honey is needed, and the inventors of the present invention develop it.

In particular, when a feed solution containing sugar is simply supplied, a compound that is toxic to bees digesting sugar or shortens the lifespan is contained. In the present invention, it is economically suitable without being toxic to bees and It is a great feature to provide specific components and specific contents that can also enhance the physiological function of

In other words, as the saccharide is an essential component for survival and reproduction of bees, sugar, fructose, glucose, and raw sugar are often selectively used as saccharides, but in the present invention, dextrose among various saccharides , maltose (maltose), trehalose (trehalose), fructose (fructose), stevia sugar, characterized in that applying a saccharide composed of a mixture of saccharin, more preferably the dextrose (dextrose), maltose ( maltose), trehalose, fructose, stevia sugar, and saccharin 0.2-0.5: 0.2-0.5: 0.2-0.5: 0.4-0.5: It is preferable to mix in a weight ratio of 1:1. . This is an essential condition that honeybees do not show toxicity in digesting sugar, and if this is exceeded, there is a concern that some of the bees are not suitable for digesting sugar, which may increase the mortality rate of bees.

The amino acids are substances added to enhance the immunity of bees, and their specific components and their specific content also include methionine, Amino acids composed of tryptophan, arginine, lysine, histidine, phenylalanine, isoleucine, threonine, leucine, and valine are applied characterized in that, more preferably the methionine, tryptophan, arginine, lysine, histidine, phenylalanine, isoleucine, threonine , leucine, valine in a weight ratio of 1-2: 1: 2-3: 2-3: 1-2: 1-2: 3-4: 2-3: 4-5: 4-5 It is better to be composed by mixing. This is an essential condition for enhancing immunity without affecting the mortality rate of bees.

The trace elements are also essential components for enhancing the immunity of bees, like the amino acids. Among the various trace elements, calcium (Ca), potassium (K), magnesium (Mg), manganese (Mn), phosphorus (P), sodium ( Na), characterized in that trace elements composed of zinc (Zn) are applied, more preferably the calcium (Ca), potassium (K), magnesium (Mg), manganese (Mn), phosphorus (P), sodium ( Na) and zinc (Zn) are preferably mixed in a weight ratio of 1: 4-5: 0.2-0.5: 0.005-0.05: 1-2: 2-3: 0.01-0.05 by weight, which also affects the mortality rate of bees. It is an essential condition to increase immunity without being too weak.

The composition for bee functional sugar solution feed configured in this way is characterized in that it provides a feed mixed with sugar water at the time of feeding the bees, thereby having an effect of enhancing the expression of antibacterial protein compared to before feed feeding.

Hereinafter, the bee functional sugar solution feed according to the present invention will be described in detail as follows.

Step 1) Preparation of bee functional sugar solution feed composition of the present invention

In this step, a bee functional sugar solution feed composition is prepared, and the feed composition is composed of sugars, amino acids, and trace elements.

In this case, it is preferable that the sugar content is 80-90 wt%, the amino acid content is 5-15 wt%, and the trace element is 0.1-5 wt% based on the total feed composition.

This is an essential condition for improving only the physiological function of bees without affecting the mortality rate of bees.

In addition, in this step, the composition may be configured to further include an additional material to obtain a more elevated immune synergistic effect. B3, vitamin B5, vitamin B7, vitamin B8, vitamin B12, vitamin B6, vitamin C, vitamin E, caffeine (caffeine), mint powder is characterized in that it is composed more, these additional substances are also very important in the mixing ratio will work More preferably, the malic acid, taurine, vitamin B, vitamin B2, vitamin B3, vitamin B5, vitamin B7, vitamin B8, vitamin B12, vitamin B6, vitamin C, vitamin E, caffeine (caffeine) ), mint powder (Candy powder) 1: 0.0005-0.001: 0.0001-0.0005: 0.0001-0.0005: 0.0001-0.0005: 0.0001-0.0005: 0.0001-0.0005: 0.0001-0.0005: 0.0001-0.0005: 0.0001-0.0005: 0.2- 0.5: 1-2: 0.002-0.005: It is preferable to mix in a weight ratio of 0.5 to 1, and the additional substances constituted in this way are mixed in the feed composition in an amount of 4 to 10% by weight based on the total weight of the feed composition. Also, as an essential condition for improving only the physiological function of bees without affecting the mortality rate of bees, there is a risk that the digestive power of the bees or the activity of the bees may be decreased if it is out of the above weight range. In particular, it can be seen that the survival rate of bees is maintained longer due to the addition of the mint powder, and thus it can be seen that the additional material exhibits higher efficacy.

Step 2) Manufacture of final honey bee functional sugar solution

In this step, the bee functional sugar solution feed composition is mixed with sugar water to prepare a feed. In this case, the feed composition is preferably mixed in an amount of 10 to 20 parts by weight based on 100 parts by weight of the sugar solution.

This is to allow the bees to balance the nutrition and energy of the bees without side effects and to improve immunity and at the same time to make it available to consumers without burdening economically, and the content of the feed composition is less than 10 parts by weight. When it is contained as a, it is difficult to obtain the desired efficacy of the present invention, and when it exceeds 20 parts by weight, there is a problem in that the number of dead insects increases without exhibiting a higher expected effect.

In addition, the sugar water is characterized in that the sugar is contained in an amount of 70 to 80% by weight, where when the content of the sugar is less than 70% by weight, the moisture content is relatively large, so that the feed, which is a basic characteristic of bees, is consumed (the queen bee is A large amount of energy is required for basic tasks such as spawning or storing honey, aquaculture, and pollen) and generating wind with wings to concentrate moisture content to less than 20% and then store and ripen. Therefore, the lifespan of the bees is shortened, and when it exceeds 80% by weight, it is difficult to obtain a more elevated effect, and it is not economically suitable.

When the honey bee functional sugar solution feed of the present invention configured in this way is fed to adult bees, it is economically suitable, so that consumers can easily use it, and at the same time, the number of dead bees is low, and in particular, Apideacin, a bee bioactive substance, As the expression of antibacterial proteins expressing Dedensin, Hymenoptacin, and Abaecin is increased, the immunity of bees is enhanced, thereby preventing the decrease in the number of bees due to climate change.

Hereinafter, the present invention will be described in more detail by way of examples, but these examples are for illustrative purposes only and are not intended to limit the protection scope of the present invention.

<Examples 1,2 and Comparative Examples 1,2,3> Preparation of bee functional sugar solution feeds

The honey bee functional sugar solution feed composition of the present invention was prepared by mixing according to the composition ratio of Table 1 below (the materials in Table 1 were purchased commercially).

sugars dextrose 31.0g maltose 31.0g trehalose 31.0g fructose 50.0g Stevia Sugar 100.0g saccharin 100.0g amino acid methionine 1.5g tryptophan 1.0g arginine 3.0g lysine 3.0g histidine 1.5g phenylalanine 1.5g isoleucine 4.0g threonine 3.0g leucine 4.5g valine 4.0g trace elements ca 107.0mg K 441.0mg Mg 40.0mg Mn 1.0mg p 129.0mg Na 251.0mg Zn 3.0mg etc
additive malic acid 6.0g taurine 5.0mg vitamin B 1.0mg B2 1.2mg B3 2.0mg B5 2.0mg B7 2.0mg B8 2.0mg B12 2.0mg pyridoxamine (B6) 2.0mg vitamin C 3.0g vitamin E 10.0g caffeine 30.0mg Park Ha-Boon 3.9g

The feed composition thus constituted was mixed with 75% (w/w) sugar water to prepare a feed, but 10-50 parts by weight (Examples 1 and 2 and comparison) based on 100 parts by weight of the sugar water in 10 liters of the sugar water Examples 1,2,3) were mixed so as to include bee functional sugar solution feeds.

<Experimental Example 1> Investigation of food preference for bee functional sugar solution composition

1. Experimental method

Worker bees of a certain age are collected from the hive, put 100 each in breeding cages, and fed with sugar substitute solution and sugar water 3 times each in the cage.

Food preference was compared by measuring changes in the contents of substitute sugar solution and sugar water fed daily, and it was confirmed that the test group with more food consumption had a higher food preference.

2. Experimental results

As shown in the experimental result in Figure 1, the food preference of the composition mixing test group did not show any significant difference by weight compared to the sugar water (control), so economically 10 to 20 parts by weight (Examples 1 and 2) It has been found that the composition is suitable for use.

<Experimental Example 2> Investigation of dead insect numbers for bee functional sugar solution composition

1. Experimental method

Worker bees of a certain age are collected from the hive, put 100 each in breeding cages, and fed with sugar substitute solution and sugar water 3 times each in the cage.

The number of dead bees in cages fed with the replacement sugar solution and sugar water daily were measured.

2. Experimental results

As a result of examining the number of dead insects by weight of the composition, the number of dead insects per day was 7 at 30 parts by weight (Comparative Example 1), 10 at 40 parts by weight (Comparative Example 2), and 13 at 50 parts by weight (Comparative Example 3). On the other hand, the composition of 10 parts by weight and 20 parts by weight (Examples 1 and 2) showed a small number of caterpillars (number of dead bees) of about 1 or 2 insects, especially the lowest number of caterpillars at 10% by weight. was confirmed to be visible.

<Experimental Example 3> Investigation of the expression level of bee bioactive substances in the bee functional sugar solution composition

1. Experimental method

Honey bees fed with alternative feed and bees fed with sugar solution were collected from cages and ground using liquid nitrogen to isolate total RNA. Using the isolated RNA as a template, cDNA was synthesized. PCR was performed using primers for quantitative analysis of Dedensin, Hymenopteacin, and Abaecin. To confirm the accuracy of the experiment, after isolation of total RNA, the expression levels of Apideacin, Dedensin, Hymenopteacin, and Abaecin were confirmed by Northern blotting.

2. Experimental results

2 and 3, it was confirmed that bees fed the bee functional sugar solution increased the expression of antibacterial proteins expressing the bee bioactive substances Apideacin, Dedensin, Hymenoptacin, and Abaecin.

As mentioned above, when the comprehensive results of the present invention are derived, it can be seen that by the present invention, as the expression of the antibacterial protein that enhances the physiological function of bees is increased, it has the effect of preventing the decrease in the number of bees due to climate change, etc. can

The present invention has been reviewed based on preferred embodiments and experimental examples, and those of ordinary skill in the art to which the present invention pertains will implement other forms than the detailed description of the present invention within the essential technical scope of the present invention Examples may be implemented. Here, the essential technical scope of the present invention is indicated in the claims, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Claims (4)

i) sugars including dextrose, maltose, trehalose, fructose, stevia sugar, and saccharin;
ii) methionine, tryptophan, arginine, lysine, histidine, phenylalanine, isoleucine, threonine, leucine, valine valine);
iii) trace elements including calcium (Ca), potassium (K), magnesium (Mg), manganese (Mn), phosphorus (P), sodium (Na) and zinc (Zn); and
iv) malic acid, taurine, vitamin B, vitamin B2, vitamin B3, vitamin B5, vitamin B7, vitamin B8, vitamin B12, vitamin B6, vitamin C, vitamin E, caffeine and mint powder ; Feed composition comprising (a); and
As a feed composition for enhancing the expression of bees antibacterial protein and reducing the mortality rate of bees, containing 75% (w/w) of sugar water (b),
The feed composition (a) is characterized in that 10 or 20 parts by weight is mixed with 100 parts by weight of the sugar water (b),
The component of the feed composition (a) is included in the content shown in Table 1 below, feed composition for enhancing the expression of bees antibacterial protein and reducing the mortality rate of bees
[Table 1]

.
According to claim 1, wherein the antibacterial protein of bees, apideacin (apideacin), didensin (dedensin), hymenopteacin (hymenopteacin) and abaecin (abaecin) antibacterial protein expression of bees, characterized in that A feed composition for promoting and reducing the mortality rate of bees.
1) i) sugars including dextrose, maltose, trehalose, fructose, stevia sugar, and saccharin;
ii) methionine, tryptophan, arginine, lysine, histidine, phenylalanine, isoleucine, threonine, leucine, valine valine);
iii) trace elements including calcium (Ca), potassium (K), magnesium (Mg), manganese (Mn), phosphorus (P), sodium (Na) and zinc (Zn); and
iv) malic acid, taurine, vitamin B, vitamin B2, vitamin B3, vitamin B5, vitamin B7, vitamin B8, vitamin B12, vitamin B6, vitamin C, vitamin E, caffeine and mint powder ; to prepare a feed composition by mixing; and
2) mixing the feed composition prepared in step 1) in an amount of 10 or 20 parts by weight based on 100 parts by weight of 75% (w/w) sugar water; A method for producing a feed composition for enhancing the expression of bees antibacterial protein and reducing the mortality rate of bees, comprising:
The component of the feed composition of step 1) is included in the content shown in Table 1 below, Method for producing a feed composition for enhancing the expression of bees antibacterial protein and reducing the mortality rate of bees
[Table 1]

. Feeding the bees with the feed composition for enhancing the expression of the antibacterial protein of the bees of claim 1 or 2 and reducing the mortality rate of bees; A method for enhancing the expression of antibacterial proteins in bees and reducing the mortality rate of bees, comprising a.

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