KR102294091B1 - Cosmetic composition comprising artichoke leaf and illite as an active ingredient, and a method of manufacturing the same - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising artichoke leaves and illite as active ingredients, which have antioxidant, skin whitening, skin elasticity enhancement, anti-inflammatory and anti-pollution effects by repurifying a mixture obtained by refining artichoke leaves and illite, respectively, and It relates to a manufacturing method thereof.

Description

Cosmetic composition comprising artichoke leaf and illite as an active ingredient, and a method of manufacturing the same

The present invention relates to a cosmetic composition comprising artichoke leaves and illite as active ingredients, and a method for manufacturing the same, and more particularly, to a mixture obtained by refining each of the artichoke leaves and illite to provide antioxidant, skin whitening, It relates to a cosmetic composition comprising, as active ingredients, artichoke leaves and illite having skin elasticity enhancement, anti-inflammatory and anti-pollution effects, and a method for preparing the same.

Human skin is constantly aging as we age. Skin aging is largely divided into natural aging and external aging. Natural aging is difficult to artificially control because it is influenced by genetic factors, but external aging is relatively easy to artificially control because it is influenced by environmental factors. . UV rays are a typical external aging factor, and the most prominent aging phenomenon is wrinkle formation, which also affects skin elasticity. Therefore, research on a cosmetic composition capable of slowing skin aging is actively being conducted.

In addition, problems such as aging or inflammation occurs in the skin exposed to contaminants, and studies on techniques for neutralizing or removing contaminants attached to the skin are being actively conducted.

On the other hand, skin exposed to UV rays produces melanin. The melanin is changed from tyrosine to dopa and dopaquinone by the action of tyrosinase present in pigment cells, and is produced through dopachrome. This melanin exists in the skin to protect the body from ultraviolet rays and has an important function in regulating the secretion of hormones in the body. However, when melanin is overproduced, it is known that it forms spots, freckles, etc., promotes skin aging, and plays an important role in inducing skin cancer, and research and development to prevent overproduction of melanin is being actively conducted.

In addition, inflammation caused by cells or inflammation such as acne occurring on the skin is a major factor that ruins the appearance of the skin. Effectively alleviating such inflammation has become an important task for skin improvement.

In order to complexly solve the above problems, research has been made on cosmetic compositions having antioxidant, skin whitening, skin elasticity enhancement, anti-inflammatory and anti-pollution effects, but conventional research has mainly been conducted using chemicals. . A cosmetic composition using a chemical may have side effects on the skin, and it is economically expensive and has problems in that it is not ecologically sustainable. Accordingly, studies on cosmetic compositions having antioxidant, skin whitening, skin elasticity enhancement, anti-inflammatory and anti-pollution effects using natural raw materials are in progress.

However, natural materials have high absorption and bioavailability in the body, but when an extract extracted from a natural material is applied to the skin, the absorption rate is low, and the antioxidant, skin whitening, skin elasticity enhancement, anti-inflammatory and anti-pollution effects do not appear satisfactorily. there was

Republic of Korea Patent Publication No. 10-1615417 (2016.04.25. Announcement), "Antioxidant, whitening and anti-inflammatory herbal cosmetic composition"

In order to solve the above problems, the present invention provides an artichoke leaf and a plant having antioxidant, skin whitening, skin elasticity enhancement, anti-inflammatory and anti-pollution effects by repurifying a mixture obtained by refining a mixture of artichoke leaves and illite, respectively. An object of the present invention is to provide a cosmetic composition comprising light as an active ingredient and a method for manufacturing the same.

In order to achieve the above object, the present invention provides an artichoke leaf extract obtained by extracting artichoke leaves with steam; A cosmetic composition comprising artichoke leaves and illite as active ingredients, characterized in that it has antioxidant, skin whitening, skin elasticity enhancement and anti-inflammatory effects by mixing and refining purified illite by grinding, heating, and solvent extraction provides

In addition, the composition is characterized in that it has an antifouling effect.

In addition, the artichoke leaf extract and purified illite are characterized in that they are mixed in a weight ratio of 30: 70 to 70: 30.

In addition, the present invention provides a steam generating step of heating purified water to generate steam (S10); an extraction steam production step (S20) of dissolving the extract from the artichoke leaves while passing the steam generated in the steam generating step (S10) between the artichoke leaves to form extraction steam (S20); an artichoke leaf extract preparation step (S30) of obtaining an artichoke leaf extract by cooling the extracted steam formed in the extracting steam manufacturing step (S20) with a cooler; Illite purification step (S40) of pulverizing illite, heating, and solvent extraction to obtain purified illite; preparing an artichoke leaf-illite mixture by mixing the artichoke leaf extract of step (S30) and the purified illite of step (S40) to prepare an artichoke leaf-illite mixture (S50); Artichoke leaf-illite mixed purified preparation step (S60) of purifying the artichoke leaf-illite mixture prepared in the step (S50) to prepare an artichoke leaf-illite mixed purified product (S60) It provides a method for manufacturing a cosmetic composition comprising artichoke leaves and illite as active ingredients.

In addition, the artichoke leaf extract and purified illite of the mixture preparation step (S50) are mixed in a weight ratio of 30:70 to 70:30.

The cosmetic composition comprising artichoke leaf and illite as active ingredients according to the present invention has antioxidant, skin whitening, skin elasticity enhancement, anti-inflammatory and anti-pollution effects by repurifying a mixture obtained by refining artichoke leaf and illite as active ingredients. has the effect of having

1 is a flowchart illustrating a manufacturing process of a cosmetic composition comprising artichoke leaves and illite as active ingredients according to the present invention.
2 is a graph showing the antioxidant effect of a cosmetic composition comprising artichoke leaves and illite as active ingredients according to the present invention.
3 is a graph showing the skin whitening effect of a cosmetic composition comprising artichoke leaves and illite as active ingredients according to the present invention.
Figure 4 is a graph showing the skin elasticity enhancing effect of the cosmetic composition comprising the artichoke leaf and illite according to the present invention as active ingredients.
5 is a graph showing the anti-inflammatory effect of a cosmetic composition comprising artichoke leaves and illite as active ingredients according to the present invention.
6 is a graph showing the antifouling effect on benzopyrene of a cosmetic composition comprising artichoke leaves and illite as active ingredients according to the present invention.
7 is a graph showing the antifouling effect on ammonium nitrate of a cosmetic composition comprising artichoke leaves and illite according to the present invention as active ingredients.
8 is a graph showing the antifouling effect on formaldehyde of a cosmetic composition comprising artichoke leaves and illite as active ingredients according to the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The following detailed description of the present invention is an embodiment in which the present invention may be practiced, and reference is made to the accompanying drawings shown by way of example of the embodiment. These embodiments are described in sufficient detail to enable those skilled in the art to practice the present invention. It should be understood that various embodiments of the present invention are different but need not be mutually exclusive. For example, certain shapes, structures, and characteristics described herein may be implemented in other embodiments without departing from the spirit and scope of the invention in relation to one embodiment. In addition, it should be understood that the position or arrangement of individual components in each described embodiment may be changed without departing from the spirit and scope of the present invention.

Accordingly, the detailed description set forth below is not intended to be taken in a limiting sense, and the scope of the present invention, if properly described, is limited only by the appended claims, along with all scopes equivalent to those claimed by the claims. Like reference numerals in the drawings refer to the same or similar functions throughout the various aspects.

The terms used in the present invention have been selected as currently widely used general terms as possible while considering the functions in the present invention, but these may vary depending on the intention or precedent of a person skilled in the art, the emergence of new technology, and the like. In addition, in a specific case, there is a term arbitrarily selected by the applicant, and in this case, the meaning will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term and the overall content of the present invention, rather than the name of a simple term.

In the present invention, when a part "includes" a certain component, it means that other components may be further included, rather than excluding other components, unless otherwise stated.

Hereinafter, a cosmetic composition comprising artichoke leaves and illite according to the present invention as active ingredients and a method for manufacturing the same will be described in detail.

A cosmetic composition comprising artichoke leaves and illite according to the present invention as active ingredients,

a steam generating step of heating purified water to generate steam (S10);

an extraction steam production step (S20) of dissolving the extract from the artichoke leaves while passing the steam generated in the steam generating step (S10) between the artichoke leaves to form extraction steam (S20);

an artichoke leaf extract preparation step (S30) of obtaining an artichoke leaf extract by cooling the extracted steam formed in the extracting steam manufacturing step (S20) with a cooler;

Illite purification step (S40) of pulverizing illite, heating, and solvent extraction to obtain purified illite;

preparing an artichoke leaf-illite mixture by mixing the artichoke leaf extract of step (S30) and the purified illite of step (S40) to prepare an artichoke leaf-illite mixture (S50);

and an artichoke leaf-illite mixed purified product preparation step (S60) of purifying the artichoke leaf-illite mixture prepared in the step (S50) to prepare an artichoke leaf-illite mixed purified product.

In the steam generating step (S10) of heating purified water to generate steam, purified water is placed in a first container and heated to 100° C. or higher to generate steam.

All materials may be used as long as the first container can heat purified water by transferring heat. However, it is appropriate to be formed of glass in order to check the inside.

The purified water refers to water from which all impurities such as dissolved ions, solid particles, organic matter, microorganisms and dissolved gas contained in water have been removed, and sterile distilled water may be appropriately used.

Next, an extraction steam production step (S20) of dissolving the extract from the artichoke leaves while passing the steam generated in the steam generating step (S10) through the artichoke leaves to form the extraction steam is performed.

In the steam extraction step (S20), the steam generated from the first container is moved to the second container in which the artichoke leaves are stored, and passes between the artichoke leaves of the second container.

At this time, the water vapor applies heat to the artichoke leaf, stimulates cells inside the artichoke leaf, and the extract is eluted from the artichoke leaf.

The artichoke ( Cynara scolymus ) is a dicotyledonous perennial plant of the order Asteraceae, native to the Mediterranean coast, and grows near the beach. The stem is 1.5 to 2 m high and resembles a thistle. The leaves are alternate phyllotaxis and deeply divided in pinnate shape. The surface of the leaf is green and the back side is densely covered with white cotton-like hairs. Flowers bloom in purple in summer and run in head-shaped flowers. The head flower was 15cm in diameter and consisted of a tubular flower. There are no thorns on the gun, and the pieces of the gun are thick. Because it likes a mild climate, it can only winter in the southern regions of Korea, and can be easily cultivated on the coast of southern regions of Korea or on Jeju Island. Before the flowers bloom, cut off the underside of the guns and eat them as vegetables or canned.

In the present invention, an artichoke leaf extract ( Cynara scolymus Leaf Extract ) is used.

The artichoke leaf contains cynacropicin, flavonoids, polyphenols, caffeoylquinic acids, sesquiterpene lactones, and the like.

In the present invention, the components inside the artichoke leaf, including the cinacrofin, flavonoids, polyphenols, caffeoylquinic acids, and sesquiterpene lactone, are purified through steam, and are purified once again together with purified illite. Antioxidant, skin whitening, skin elasticity enhancement, anti-inflammatory and anti-pollution effects can be enhanced.

In the step (S20), the artichoke leaves may be fresh leaves or dried artichoke leaves. This may be changed according to the practice of those skilled in the art, and both types of artichoke leaves may be included in the present invention.

The water vapor that has passed between the artichoke leaves in the step S20 is changed to the extracted vapor containing the artichoke leaf extract.

In the step (S20), the artichoke leaf may have undergone a fermentation process. For example, the artichoke leaves may be fermented using Saccharomyces Cerevisiae species. The strain may be commercially available as a publicly disclosed strain. When the fermentation process is performed on the artichoke leaves, the antioxidant, skin whitening, skin elasticity enhancement, anti-inflammatory and anti-pollution effects of the finished composition can be further increased.

In the step (S20), a weak rotational air flow may be applied to the water vapor. If a rotating airflow is applied to the water vapor, the water vapor will penetrate deep between the artichoke leaves, allowing the ingredients inside the artichoke leaves to come out more easily into the water vapor.

Next, an artichoke leaf extract preparation step (S30) of obtaining an artichoke leaf extract is performed by cooling the extracted steam formed in the extracting steam manufacturing step (S20) with a cooler.

In the step (S30), by moving the extracted steam to the cooler, the extracted steam is condensed by the cooling water flowing around the cooling pipe, and the extract can be prepared.

The cooler may use various types of products, and it will be appropriate that the cooling tube is spirally rolled in order to increase the cooling time of the extraction steam.

It is appropriate that the cooling water has a temperature of 1 to 10 °C. It is recommended to use low-temperature water for smooth extraction of the extraction steam.

Next, the illite purification step (S40) of pulverizing illite, heating, and solvent extraction to obtain purified illite is performed.

The illite refining step (S40) includes a first grinding step (S41) of pulverizing the collected illite; a second grinding step (S42) of heating the illite pulverized through the first pulverizing step to 500° C. and then pulverizing; A mixing step (S43) of mixing the illite pulverized through the second grinding step with ethanol in an amount of 50% by weight: 50% by weight; a purification step (S44) of purifying the illite and the ethanol mixed through the mixing step; Separation step of separating the purified illite and the ethanol (S45); It may include a drying step (S46) of drying the illite separated through the separation step.

The illite is a secondary mineral commonly found in the weatherings of heterogeneous sedimentary rocks, hydrothermal transition zones, and jupiter-acid rocks. The illite is ^{well formed in an environment rich in K +,} is easily formed from feldspar or mica, or is directly precipitated from a hot water solution. Illite is the most abundant clay mineral in shale and sandstone along with kaolinite and illite-smectite mixed layered minerals. In a general deposition environment, as the rapid action progresses, the clay minerals start from smectite, go through the I/S mixed lamellar minerals, and finally transition to illite.

In the present invention, the illite that has passed through the steps (S41) to (S46) is referred to as 'refined illite'.

Next, the artichoke leaf-illite mixture preparation step (S50) of preparing an artichoke leaf-illite mixture by mixing the artichoke leaf extract of step (S30) and the purified illite of the step (S40) is performed do.

In the step (S50), the artichoke leaf extract and purified illite are mixed in a weight ratio of 30:70 to 70:30 to prepare an artichoke leaf-illite mixture.

The weight ratio is an appropriate mixing value so that the cosmetic composition comprising the artichoke leaf and illite according to the present invention as an active ingredient has an optimal effect on antioxidant, skin whitening, skin elasticity enhancement, anti-inflammatory and anti-pollution effects.

Next, the artichoke leaf-illite mixed purified product preparation step (S60) of purifying the artichoke leaf-illite mixture prepared in the step (S50) to prepare an artichoke leaf-illite mixed purified product is performed .

In the step (S60), the antioxidant, skin whitening, skin elasticity enhancement, anti-inflammatory and anti-pollution effects of the composition of the present invention can be maximized by repurifying the artichoke leaves and illite, which were extracted or purified separately, once again. .

The step (S60) may be performed in a vacuum state. When the purification process is performed in a vacuum, antioxidant, skin whitening, skin elasticity enhancement, anti-inflammatory and anti-pollution effects can be further maximized.

After the above manufacturing process, a cosmetic composition comprising the artichoke leaf and illite of the present invention as an active ingredient is completed, and it can be applied by including it in a cosmetic formulation.

A cosmetic composition comprising artichoke leaves and illite as active ingredients according to the present invention comprises: an artichoke leaf extract obtained by extracting artichoke leaves with steam; By mixing and refining the purified Illite by grinding, heating, and solvent extraction, the artichoke leaf extract and the purified Illite are reacted through the reaction between the two substances to enhance antioxidant, skin whitening, and skin elasticity. It is characterized in that the anti-inflammatory effect is increased.

In addition, the cosmetic composition comprising the artichoke leaf and illite according to the present invention as active ingredients has an antifouling effect.

Hereinafter, a cosmetic composition comprising an artichoke leaf and illite according to the present invention as an active ingredient will be described in detail through Examples, Comparative Examples and Experimental Examples.

Examples 1 to 5. Artichoke leaf-Illite mixed tablet preparation

Through the following manufacturing process, artichoke leaf-illite mixed tablets were prepared.

Steam generation step (S10): purified water was heated to generate steam.

Extracted steam production step (S20): while passing the steam generated in the steam generating step (S10) between the artichoke leaves, the extract was dissolved from the artichoke leaves to form extracted steam.

Artichoke leaf extract preparation step (S30); The extracted steam formed in the steam extraction step (S20) was cooled with a cooler to obtain an artichoke leaf extract.

Illite purification step (S40): Illite was pulverized, heated, and solvent extracted to obtain purified Illite.

Artichoke leaf-illite mixture preparation step (S50): The artichoke leaf extract of step (S30) and the purified illite of step (S40) were mixed in the weight ratio shown in Table 1 below to prepare an artichoke leaf-illite mixture prepared.

Artichoke leaf-illite mixed purified preparation step (S60): The artichoke leaf-illite mixture prepared in the above step (S50) was purified to prepare an artichoke leaf-illite mixed purified product.

Weight ratio of artichoke leaf extract
(weight%) Weight ratio of purified illite
(weight%) Example 1 10 90 Example 2 30 70 Example 3 50 50 Example 4 70 30 Example 5 90 10

Comparative Example 1. Preparation of Artichoke Leaf Extract

Artichoke leaf extract was prepared through the following manufacturing process.

Steam generation step (S10): purified water was heated to generate steam.

Extracted steam production step (S20): while passing the steam generated in the steam generating step (S10) between the artichoke leaves, the extract was dissolved from the artichoke leaves to form extracted steam.

Artichoke leaf extract preparation step (S30); The extracted steam formed in the step (S20) of preparing the extracted steam was cooled with a cooler to obtain an artichoke leaf extract.

Comparative Example 2. Preparation of purified illite

Purified illite was manufactured through the following manufacturing process.

First crushing step (S41): The collected illite was crushed.

Second crushing step (S42): The illite pulverized through the first crushing step was heated to 500° C. and then pulverized.

Mixing step (S43): The illite pulverized through the second grinding step was mixed with ethanol in an amount of 50% by weight: 50% by weight.

Purification step (S44): The illite and the ethanol mixed through the mixing step were purified.

Separation step (S45): The purified illite and the ethanol were separated.

Drying step (S46): The illite separated through the separation step was dried.

Comparative Example 3. Preparation of artichoke leaf-illite mixture

An artichoke leaf-illite mixture was prepared through the following manufacturing process.

Steam generation step (S10): purified water was heated to generate steam.

Extracted steam production step (S20): while passing the steam generated in the steam generating step (S10) between the artichoke leaves, the extract was dissolved from the artichoke leaves to form extracted steam.

Artichoke leaf extract preparation step (S30); The extracted steam formed in the steam extraction step (S20) was cooled with a cooler to obtain an artichoke leaf extract.

Illite purification step (S40): Illite was pulverized, heated, and solvent extracted to obtain purified Illite.

Artichoke leaf-illite mixture preparation step (S50): The artichoke leaf extract of step (S30) and the purified illite of step (S40) were mixed in a 50:50 weight ratio to prepare an artichoke leaf-illite mixture did.

Experimental Example 1. Confirmation of DPPH radical scavenging ability

Free radical scavenging activity was measured according to the method of Blois (Blois MS. Nature 1958, 181, 1199-1202). In 0.8 ml of 0.4 mM DPPH solution, 0.2 ml of a liquid solution of Examples 1 to 5 and Comparative Examples 1 to 3 dissolved in distilled water at a concentration of 5 mg/ml was added, and the absorbance was measured at 540 nm after standing for 10 minutes. DPPH radical scavenging ability was calculated according to the following equation.

DPPH scavenging activity (%)=[(A ₀ -A ₁ )/A ₀ ]×100

A ₀ : Absorbance of the control group, A ₁ : Absorbance of the sample

DPPH scavenging activity (%) of 5 mg/ml of Examples 1 to 5 and Comparative Examples 1 to 3 Example 1 60.8 Example 2 75.4 Example 3 83.8 Example 4 73.2 Example 5 59.4 Comparative Example 1 32.3 Comparative Example 2 10.4 Comparative Example 3 52.8

As a result, the highest DPPH scavenging ability was confirmed in Example 3, in which the artichoke leaf extract and purified illite were mixed in a weight ratio of 50:50 and purified.

In addition, Example 2, which was purified by mixing artichoke leaf extract and purified illite in a weight ratio of 30:70, and Example 4, in which artichoke leaf extract and purified illite were mixed and purified in a weight ratio of 70:30, were also excellent. DPPH scavenging ability was confirmed.

However, Example 1, in which the artichoke leaf extract and purified illite were mixed in a weight ratio of 10:90, and Example 5, in which the artichoke leaf extract and purified illite were mixed and purified in a weight ratio of 90:10, were somewhat It showed a low level of DPPH scavenging ability.

Therefore, it was confirmed that the appropriate mixing ratio of the artichoke leaf extract of the present invention and the purified illite was 30:70 to 70:30 by weight.

On the other hand, Comparative Example 1, which is a single extract obtained by simply extracting artichoke leaves, and Comparative Example 2, which is only purified by illite, showed a low level of DPPH scavenging ability, and Comparative Example 3, which was a mixture of the two, also showed a low level of DPPH scavenging ability.

In particular, it was confirmed that the DPPH scavenging ability of Examples 1 to 5 was higher than the sum of the sum of the DPPH scavenging powers of Comparative Example 1 and Comparative Example 2.

Accordingly, the cosmetic composition comprising the artichoke leaf and illite of the present invention as an active ingredient is an artichoke leaf extract obtained by extracting artichoke leaves with steam and illite by grinding and heating the purified illite obtained by mixing and refining the solvent. It was confirmed that unexpected effects occurred during the process.

Therefore, the cosmetic composition comprising the artichoke leaf and illite of the present invention as an active ingredient removes at least 73.2% to a maximum of 83.8% of DPPH by mixing the artichoke leaf extract and purified illite in a weight ratio of 30: 70 to 70: 30 It is characterized in that it exhibits activity.

Experimental Example 2. Melanin production inhibitory ability test

After putting 5-6 ml of DMEM medium (glucose 4.5g/L 89%, serum 10%, antibiotic 1%) in a 25 cm 2 T-flask, cryopreserved mouse-derived B-16 melanoma (ATCC CRL 6323) cell line After inoculation and incubation for 24 hours under 37°C temperature and 5% CO ₂ conditions, the cells were separated by treatment with 0.05% trypsin containing 0.02% EDTA, and then inoculated into a 25 cm 2 T-flask and cultured for 48 hours. . As a result, the number of cells obtained was 5.76 × 10 ⁶ cells per flask. Here, 100 μg/ml of Examples 1 to 5 and Comparative Examples 1 to 3 were diluted in DMEM medium and treated with cultured melanoma cells and cultured at 37° C. for 5 days. After culture, all of the medium was removed, and cells were separated by treatment with 1 ml of saline-phosphate buffer (PBS) containing 0.02% EDTA and 0.05% trypsin, followed by centrifugation for 5 minutes to collect only the cells. The collected cells were treated with 5% trichloroacetate (TCA), stirred, and centrifuged to wash the precipitated melanin with a saline-phosphate buffer solution. Then, after dissolving the precipitated melanin by treatment with 1N NaOH, absorbance was measured at 475 nm, and the concentration of melanin was determined from the standard concentration curve of synthetic melanin (Sigma), and the inhibition of melanin production was shown in Table 3 below.

Melanin production inhibition rate (%) Example 1 43.4 Example 2 61.7 Example 3 72.5 Example 4 59.2 Example 5 42.5 Comparative Example 1 32.6 Comparative Example 2 24.3 Comparative Example 3 38.2

As a result, the highest melanin production inhibition rate was confirmed in Example 3, in which the artichoke leaf extract and purified illite were mixed in a weight ratio of 50:50 and purified.

In addition, Example 2, in which the artichoke leaf extract and purified illite were mixed in a weight ratio of 30:70, and Example 4, in which the artichoke leaf extract and purified illite were mixed and purified in a weight ratio of 70:30, were also excellent. The inhibition rate of melanin production was confirmed.

However, Example 1, in which the artichoke leaf extract and purified illite were mixed in a weight ratio of 10:90, and Example 5, in which the artichoke leaf extract and purified illite were mixed and purified in a weight ratio of 90:10, were somewhat It showed a low level of inhibition of melanogenesis.

Therefore, it was confirmed that the appropriate mixing ratio of the artichoke leaf extract of the present invention and the purified illite was 30:70 to 70:30 by weight.

On the other hand, Comparative Example 1, which is a single extract obtained by simply extracting artichoke leaves, and Comparative Example 2, which is only purified by illite, showed a low level of inhibition of melanin production, and Comparative Example 3, in which the two were mixed, also showed a low level of inhibition of melanogenesis. showed

Accordingly, the cosmetic composition comprising the artichoke leaf and illite of the present invention as an active ingredient is an artichoke leaf extract obtained by extracting artichoke leaves with steam and illite by grinding and heating the purified illite obtained by mixing and refining the solvent. It was confirmed that unexpected effects occurred during the process.

Therefore, the cosmetic composition comprising the artichoke leaf and illite of the present invention as an active ingredient produces a minimum of 59.2% to a maximum of 72.5% of melanin by mixing the artichoke leaf extract and the purified illite in a weight ratio of 30: 70 to 70: 30 It is characterized in that it represents the inhibition rate.

Experimental Example 3. Collagen biosynthesis promoting effect test

In order to examine the effects on the skin of Examples 1 to 5 and Comparative Examples 1 to 3, the effect of promoting collagen biosynthesis was examined by an in vitro test method. The amount of collagen synthesis was measured by PIP (Procollagen Type I C-peptide) EIA assay, and the most synthesized type I precursor collagen C-terminal peptide (PICP) was quantitatively measured.

^{1×10 4} cells were seeded in a 24-well plate and cultured for 24 hours. After culturing, the medium was replaced with a serum-free medium, and Examples 1 to 5 and Comparative Examples 1 to 3 were added at a concentration of 50 μg/ml, and cultured at 37° C., 5% CO 2 in an incubator for 3 days. As a control, vitamin C, which is known to promote collagen synthesis, was treated at a concentration of 50 μg/ml in each well as a control.

The culture supernatant medium was centrifuged and used in the kit (PIP EIA KIT). A peroxidase-labeled antibody-POD conjugate (MK1O1, TAKARA BIO INC.) was put into a 96-well plate coated with a mouse monoclonal antibody against PIP and reacted, and 20 μl/well of the culture solution was added thereto. added.

After reacting for 3 hours in an incubator at 37 °C and 5% CO2, 100 μl/well of ρ-phenylenediamine, a substrate solution, was added to develop color. After standing at room temperature for 15 minutes, 100 μl/well of the reaction stop solution was added to stop the reaction. Absorbance was measured at 450 nm within 5 minutes, and the amount of collagen was measured by making a graph with the PICP standard concentration, and the results are shown in Table 4 below.

Collagen synthesis capacity (ng/ml) Example 1 244 Example 2 375 Example 3 403 Example 4 363 Example 5 246 Comparative Example 1 164 Comparative Example 2 137 Comparative Example 3 228

As a result, the highest collagen synthesis ability was confirmed in Example 3, in which the artichoke leaf extract and purified illite were mixed in a weight ratio of 50:50 and purified.

In addition, Example 2, which was purified by mixing artichoke leaf extract and purified illite in a weight ratio of 30:70, and Example 4, in which artichoke leaf extract and purified illite were mixed and purified in a weight ratio of 70:30, were also excellent. Collagen synthesis ability was confirmed.

However, Example 1, in which the artichoke leaf extract and purified illite were mixed in a weight ratio of 10:90, and Example 5, in which the artichoke leaf extract and purified illite were mixed and purified in a weight ratio of 90:10, were somewhat It showed a low level of collagen synthesis ability.

Therefore, it was confirmed that the appropriate mixing ratio of the artichoke leaf extract of the present invention and the purified illite was 30:70 to 70:30 by weight.

On the other hand, Comparative Example 1, which is a single extract obtained by simply extracting artichoke leaves, and Comparative Example 2, which is a pure extract of Illite, showed a low level of collagen synthesis ability, and Comparative Example 3, in which the two were mixed, also showed a low level of collagen synthesis ability. showed

Accordingly, the cosmetic composition comprising the artichoke leaf and illite of the present invention as an active ingredient is an artichoke leaf extract obtained by extracting artichoke leaves with steam and illite by grinding and heating the purified illite obtained by mixing and refining the solvent. It was confirmed that unexpected effects occurred during the process.

Therefore, the cosmetic composition comprising the artichoke leaf and illite of the present invention as an active ingredient is obtained by mixing the artichoke leaf extract and the purified illite in a weight ratio of 30: 70 to 70: 30 by weight of a minimum of 363 ng/ml to a maximum of 403 ng/ml. It is characterized in that it exhibits collagen synthesis ability.

Experimental Example 4. Anti-inflammatory effect

In order to confirm the skin trouble alleviation function of the anti-inflammatory effect of the compositions of the present invention prepared in Examples 1 to 5 and Comparative Examples 1 to 5, a PGE2 production inhibitory effect test was performed. (Jeffrey, KH, Anglea, SW, Zoe) , S, and Rhia CM, Intracellular Measurement of Prostaglandin E2: Effect of antiinflammatory drugs on cyclooxygenase activity and prostanoid expression, Analytical Biochemistry, 1999, 271, 18-28). After subculture of macrophage-type RAW 264.7 cells several times, 1 X 10 ⁵ each was placed in a 24-well plate and cultured for 24 hours in a 5% CO2 thermostat. After removing the medium, 50 μg of the compositions of Examples 1 to 5 and Comparative Examples 1 to 3 and a new medium containing 450 μg of LPS (1 μg/ml) were simultaneously treated and cultured under the same conditions as the pre-culture. After 24 hours, the culture medium was centrifuged (12,000 rpm, 3 min) to measure PGE2 to obtain a supernatant. The measurement of PGE2 was quantified using Enzyme-Linked Immunosorbent Assay (ELISA) to determine the prostaglandin inhibitory effect of Examples and Comparative Examples, and the results are shown in Table 5.

Prostaglandin inhibition rate (%) Example 1 56.3 Example 2 78.6 Example 3 83.4 Example 4 76.2 Example 5 57.1 Comparative Example 1 23.4 Comparative Example 2 37.2 Comparative Example 3 53.1

As a result, the highest prostaglandin inhibition rate was confirmed in Example 3, in which the artichoke leaf extract and purified illite were mixed in a weight ratio of 50:50 and purified.

In addition, Example 2, which was purified by mixing artichoke leaf extract and purified illite in a weight ratio of 30:70, and Example 4, in which artichoke leaf extract and purified illite were mixed and purified in a weight ratio of 70:30, were also excellent. Prostaglandin inhibition was confirmed.

However, Example 1, in which the artichoke leaf extract and purified illite were mixed in a weight ratio of 10:90, and Example 5, in which the artichoke leaf extract and purified illite were mixed and purified in a weight ratio of 90:10, were somewhat It showed a low level of prostaglandin inhibition.

Therefore, it was confirmed that the appropriate mixing ratio of the artichoke leaf extract of the present invention and the purified illite was 30:70 to 70:30 by weight.

On the other hand, Comparative Example 1, which is a single extract obtained by simply extracting artichoke leaves, and Comparative Example 2, which is a pure extract of Illite, showed a low level of prostaglandin inhibition, and Comparative Example 3, which was a mixture of the two, also showed a low level of inhibition of prostaglandin.

Accordingly, the cosmetic composition comprising the artichoke leaf and illite of the present invention as an active ingredient is an artichoke leaf extract obtained by extracting artichoke leaves with steam and illite by grinding and heating the purified illite obtained by mixing and refining the solvent. It was confirmed that unexpected effects occurred during the process.

Therefore, the cosmetic composition comprising artichoke leaf and illite of the present invention as an active ingredient mixes the artichoke leaf extract and purified illite in a weight ratio of 30: 70 to 70: 30, so that a prostaglandin inhibition rate of at least 76.2% to a maximum of 83.4% characterized in that it represents

Experimental Example 5. Anti-Pollution Effect

In general, the degree of cell damage caused by pollutants is measured using skin keratinocytes that come in direct contact with pollutants, and Examples 1 to 5 and Comparative Examples 1 to 3 are treated by concentration, and the effect of inhibiting skin cell damage is obtained. Confirmed. The experiment was carried out according to a known method.

Human keratinocytes HaCaT at a ^{concentration of 5 X 10 3} cells/ml were dispensed in a 96-well plate and cultured for 24 hours, followed by benzopyrene (10 μM), ammonium nitrate (500 μg/ml), and formaldehyde (1.5 μg/ml). Each of Examples 1 to 5 and Comparative Examples 1 to 3 were treated, and further cultured for 48 hours. After that, the MTT solution of 5 mg/ml concentration was added and cultured for 3 hours. Then, the medium was removed, 150ul of dimethyl sulfoxide was added to each well, shaken for 10 minutes, and absorbance was measured at 570nm.

Benzopyrene (10 μM) cell growth rate Example 1 52.4 Example 2 62.7 Example 3 72.3 Example 4 65.3 Example 5 51.5 Comparative Example 1 19.1 Comparative Example 2 37.4 Comparative Example 3 48.7

Ammonium nitrate (500 μg/ml) cell growth rate Example 1 54.2 Example 2 64.1 Example 3 74.7 Example 4 67.3 Example 5 55.9 Comparative Example 1 17.7 Comparative Example 2 38.6 Comparative Example 3 53.3

Formaldehyde (1.5 μg/ml) cell growth rate Example 1 50.2 Example 2 68.3 Example 3 69.0 Example 4 63.7 Example 5 48.4 Comparative Example 1 12.1 Comparative Example 2 33.3 Comparative Example 3 46.4

As a result, the highest cell proliferation rate was confirmed in Example 3, in which the artichoke leaf extract and purified illite were mixed in a weight ratio of 50:50 and purified.

In addition, Example 2, which was purified by mixing artichoke leaf extract and purified illite in a weight ratio of 30:70, and Example 4, in which artichoke leaf extract and purified illite were mixed and purified in a weight ratio of 70:30, were also excellent. The cell proliferation rate was confirmed.

However, Example 1, in which the artichoke leaf extract and purified illite were mixed in a weight ratio of 10:90, and Example 5, in which the artichoke leaf extract and purified illite were mixed and purified in a weight ratio of 90:10, were somewhat It showed a low cell proliferation rate.

Therefore, it was confirmed that the appropriate mixing ratio of the artichoke leaf extract of the present invention and the purified illite was 30:70 to 70:30 by weight.

On the other hand, Comparative Example 1, which is a single extract obtained by simply extracting artichoke leaves, and Comparative Example 2, which is a pure extract of Illite, showed a low cell proliferation rate, and Comparative Example 3, in which the two were mixed, also showed a low cell proliferation rate.

Accordingly, the cosmetic composition comprising the artichoke leaf and illite of the present invention as an active ingredient is an artichoke leaf extract obtained by extracting artichoke leaves with steam and illite by grinding and heating the purified illite obtained by mixing and refining the solvent. It was confirmed that unexpected effects occurred during the process.

Therefore, the cosmetic composition comprising the artichoke leaf and illite of the present invention as an active ingredient contains benzopyrene, ammonium nitrate, and formaldehyde when the artichoke leaf extract and purified illite are mixed in a weight ratio of 30: 70 to 70: 30. It was confirmed that it has an anti-pollution effect on pollutants in the air, including

conclusion.

Through the Comparative Examples and Experimental Examples, the cosmetic composition comprising the artichoke leaf and illite of the present invention as an active ingredient was prepared by pulverizing, heating, and solvent-extracting the artichoke leaf extract and illite obtained by extracting the artichoke leaves with steam. It was confirmed that an unexpected effect occurred in the process of mixing and refining the purified illite.

In addition, it was confirmed that an appropriate mixing ratio of the artichoke leaf extract of the present invention and the purified illite was 30:70 to 70:30 by weight.

In addition, the cosmetic composition comprising artichoke leaf and illite of the present invention as an active ingredient removes at least 73.2% to max of 83.8% of DPPH by mixing artichoke leaf extract and purified illite in a weight ratio of 30: 70 to 70: 30 It was confirmed that the activity was shown. From this, the antioxidant effect could be inferred.

In addition, the cosmetic composition comprising the artichoke leaf and illite of the present invention as an active ingredient produces a minimum of 59.2% to a maximum of 72.5% of melanin by mixing the artichoke leaf extract and the purified illite in a weight ratio of 30: 70 to 70: 30 It was confirmed that the inhibition rate was shown. From this, the skin whitening effect could be inferred.

In addition, the cosmetic composition comprising artichoke leaf and illite of the present invention as an active ingredient is obtained by mixing artichoke leaf extract and purified illite in a weight ratio of 30: 70 to 70: 30 by weight ratio of at least 363 ng/ml to a maximum of 403 ng/ml. It was confirmed that the collagen synthesis ability was shown. From this, the skin elasticity enhancement effect could be inferred.

In addition, the cosmetic composition comprising artichoke leaf and illite of the present invention as an active ingredient mixes artichoke leaf extract and purified illite in a weight ratio of 30: 70 to 70: 30, so that a prostaglandin inhibition rate of at least 76.2% to a maximum of 83.4% It could be confirmed that the From this, the anti-inflammatory effect could be leaked.

In addition, the cosmetic composition comprising artichoke leaf and illite of the present invention as an active ingredient contains benzopyrene, ammonium nitrate, and formaldehyde when the artichoke leaf extract and purified illite are mixed in a weight ratio of 30: 70 to 70: 30. It was confirmed that it has an anti-pollution effect on pollutants in the air, including From this, it could be inferred that it had an antifouling effect.

Accordingly, the present invention specifies that a cosmetic composition comprising artichoke leaves and illite as active ingredients has been developed as a novel material having antioxidant, skin whitening, skin elasticity enhancement, anti-inflammatory and anti-pollution effects.

Although the present invention has been described with the accompanying drawings, this is only one embodiment of various embodiments including the gist of the present invention, and is intended to be easily implemented by those of ordinary skill in the art. For the purpose, it is clear that the present invention is not limited to the embodiments described above. Accordingly, the protection scope of the present invention should be construed by the following claims, and all technical ideas within the equivalent range by changes, substitutions, substitutions, etc. within the scope not departing from the gist of the present invention are in the right of the present invention. will be included In addition, it is clarified that some components of the drawings are provided in an exaggerated or reduced form than in reality for more clearly explaining the configuration.

Claims (5)

an artichoke leaf extract obtained by extracting artichoke leaves with steam; An artichoke leaf-illite mixture obtained by mixing purified illite by grinding, heating and solvent extraction; 30: 70 to 70: 30 by weight; A cosmetic composition comprising artichoke leaves and illite as active ingredients, characterized in that it has antioxidant, skin whitening, skin elasticity enhancement and anti-inflammatory effects, including water. According to claim 1,
The composition is a cosmetic composition comprising artichoke leaves and illite as active ingredients, characterized in that it has an antifouling effect. delete a steam generating step of heating purified water to generate steam (S10);
an extraction steam production step (S20) of dissolving the extract from the artichoke leaves while passing the steam generated in the steam generating step (S10) between the artichoke leaves to form extraction steam (S20);
An artichoke leaf extract preparation step (S30) of obtaining an artichoke leaf extract by cooling the extracted steam formed in the extracting steam manufacturing step (S20) with a cooler;
Illite purification step (S40) of pulverizing illite, heating, and solvent extraction to obtain purified illite;
Artichoke leaf-illite mixture prepared by mixing the artichoke leaf extract of step (S30) and the purified illite of step (S40) in a weight ratio of 30:70 to 70:30 to prepare an artichoke leaf-illite mixture manufacturing step (S50);
Including a; A method for manufacturing a cosmetic composition comprising artichoke leaves and illite as active ingredients, characterized in that delete

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