KR101615417B1 - Korean herb cosmetic composition with the anti-oxidant, whitening and ant-inflammatory effect - Google Patents

Abstract

The present invention relates to a herbal cosmetic composition for antioxidant, whitening and anti-inflammation, and more particularly to a herbal cosmetic composition having antioxidant, whitening and anti-inflammatory properties, Wherein the composition comprises at least one extract selected from the group consisting of seeds, yamamatsu, corn oil, linseed oil, ground oil,

Description

TECHNICAL FIELD [0001] The present invention relates to a herbal cosmetic composition for antioxidant, whitening and anti-inflammation,

The present invention relates to a herbal cosmetic composition for antioxidant, whitening and anti-inflammation comprising at least one extract selected from the group consisting of green tea leaves, green tea seeds, yamamatsu, marine oil, bingo jar, ground oil,

Skin aging can be divided into two types: chronologic aging, which is accompanied by the progressive deterioration of the structure and function of the skin as time elapses, and the other is the exogenous aging (photoaging) And changes in skin texture due to long-term exposure to ultraviolet light. This aging process causes the skin to become dry, wrinkled and lose its elasticity. Free radicals and reactive oxygen species are the most important treatments in skin aging research. Although they are naturally produced in vivo, they are caused by exposure to ultraviolet light or contact with various chemicals. These free radicals and reactive oxygen species add oxidative stress to the skin cells and ultimately promote the production of melanin and wrinkles in the skin.

In addition, pigmentation in the skin is caused by melanogenesis or melanocyte proliferation, and such pigmentation can be inhibited by various methods. Commonly used methods include various methods such as suppression of tyrosinase activity, an important enzyme of melanin synthesis, reduction of melanocyte function, reduction of melanin production through inhibition of autoxidation reaction, and suppression of inflammatory reaction such as erythema have.

As a result, in order to prevent aging of the skin, it is necessary to inhibit the production of excess reactive oxygen species and efficiently remove the produced active oxygen species, and to inhibit enzymes that cause skin wrinkles, It is necessary to have an associated system.

On the other hand, inflammation is a series of defenses aimed at minimizing the response of a cell or tissue to damage by any cause and restoring the damaged area to the original state. The inflammation is caused by physical, physical, Factors, chemical factors due to chemicals, and immunological factors due to antibody responses, as well as blood vessel and hormone imbalances. Removal of inflammation to eliminate the cause of inflammation, vital reaction and symptomatic action is called anti-inflammatory action.

In order to block the oxidation, delay the aging caused by the oxidation, inhibit pigmentation of the skin, and search for a component showing anti-inflammatory activity, natural natural plants were searched and found to have free radical scavenging effect and melanin Production inhibitory effect and anti-inflammatory activity effect by inhibition of TNF-α production. Thus, the present invention was confirmed by confirming the possibility of its application as a natural material for cosmetics for antioxidation, whitening and anti-inflammation.

It is intended to provide a natural plant extract which inhibits pigment deposition or aging of skin caused by various factors proceeding from the skin, has antioxidative and antiinflammatory effects, and does not cause other side effects, and a cosmetic composition containing it as an active ingredient have.

The present invention provides antioxidant, whitening and anti-inflammatory herbal cosmetic compositions comprising one or more extracts selected from the group consisting of green tea leaves, green tea seeds, yamamatsu, marine oils, bingo jars, ground oils,

The present invention provides an antioxidant, whitening and anti-inflammatory herbal cosmetic composition comprising green tea leaf extract and green tea seed extract.

The present invention provides an antioxidant, whitening and anti-inflammatory herbal cosmetic composition comprising a crude oil extract, an extract and a herbal extract.

The present invention provides an antioxidant, whitening and anti-inflammatory herbal cosmetic composition comprising an extract of a mustard extract and a extract of corn oil.

The present invention provides an antioxidant, whitening and anti-inflammatory herbal cosmetic composition comprising a green tea leaf extract, a green tea seed extract, a crude oil extract, a chrysanthemum extract, a yambaekisan extract and a corn oil extract.

In the present invention, the weight ratio of the green tea seed extract to the green tea leaf extract is 1: 0.3 to 3, the weight ratio of the extract to the crude oil extract is 1: 0.3 to 3, To 3 < / RTI >

In the present invention, the herbal cosmetic composition may be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a water lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisture cream, a hand cream, , A soap, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion and a body cleanser.

Hereinafter, the present invention will be described in detail.

The plant extract which is an effective ingredient of the antioxidant, whitening and anti-inflammatory herbal cosmetic composition of the present invention is a plant extract which is an effective ingredient of the antioxidant, whitening and anti-inflammatory composition of the present invention, which comprises a) green tea leaves, green tea seeds, (B) finely ground powder of each material, and then b) water containing purified water, C1 to C4 lower alcohol such as methanol, ethanol, etc., butylene glycol, propylene glycol, glycerin, At least one solvent selected from the group consisting of methanol, ethanol, isobutylene glycol, water-containing propylene glycol, hexane, ethyl acetate and the like is added and the mixture is subjected to warming extraction at 10 to 200 ° C for 1 to 10 times for 5 to 80 hours, , c) may be obtained by concentration under reduced pressure in a water bath to increase the component content of the filtered extract.

The antioxidant, whitening and anti-inflammatory herbal cosmetic composition of the present invention may contain green tea leaf extract and green tea seed extract, or may contain an extract of chrysanthemum, an extract of chrysanthemum, an extract of yamgwan, and a extract of marine oil or a green tea leaf extract, , An oil extract, an acerola extract, a yamaguchi extract, and a corn oil extract.

In the present invention, the weight ratio of the green tea seed extract to the green tea leaf extract is 1: 0.3 to 3, preferably 1: 0.6 to 1.5, and the weight ratio of the extract to the crude oil extract is 1: 0.3 to 3, : 0.6 to 1.5, and the weight ratio of corn oil extract to the gazebo extract is 1: 0.3 to 3, preferably 1: 0.6 to 1.5.

The herbal cosmetic composition of the present invention can be variously used for cosmetics and cleansing agents for antioxidant, whitening and anti-inflammatory effects of the skin. Examples of products to which the present composition can be added include cosmetics such as various creams, lotions, and skins, and cleansing, cleansing agents, soaps, treatments, and essences.

The cosmetic composition of the present invention may contain, in addition to the above extracts, a skin whitening agent such as a moisturizing agent, a thickener, a surfactant, a oil base agent, an antiseptic, an antioxidant, an alcohol, a flavor, a pH adjuster, And anti-aging ingredients can be compounded at a conventional use amount in the art.

In addition, the components that may be added are not limited thereto, and any of the above components may be compounded within a range that does not impair the objects and effects of the present invention.

The antioxidant, whitening and anti-inflammatory herbal cosmetic compositions of the present invention can be prepared into any of the formulations conventionally manufactured in the art, and can be used in various forms such as emulsion, cream, lotion, pack, foundation, lotion, . Specifically, the cosmetic composition of the present invention can be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a water lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisture cream, a hand cream, Packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

Since the present invention has free radical elimination, inhibition of tyrosinase activity, inhibition of melanin formation, and inhibition of TNF-a activity, it can be used as a cosmetic composition for antioxidant, whitening and anti-inflammation.

1 shows the DPPH free radical scavenging activity of green tea leaves and green tea seed extract.
Fig. 2 shows the DPPH free radical scavenging activity of the crude oil, crude oil and bingo extract.
Fig. 3 shows the DPPH free radical scavenging activity of the extracts of the gills, chrysanthemum, and yambaek.
Fig. 4 shows the DPPH free radical scavenging activity of the cinnabar, the uniaxial, and the squam cell extract.
Figure 5 shows the superoxide quenching activity of natural plant extracts.
Figure 6 shows the DPPH free radical scavenging activity of groups A to D;
Figure 7 shows the superoxide quenching activity of Groups A to D;
Figure 8 shows the xanthine originated superoxide quenching activities of the groups A to D.
Fig. 9 shows the tyrosinase inhibitory activity of Groups A to D. Fig.
Fig. 10 shows the melanin synthesis inhibitory effect of the groups A to D. Fig.
Fig. 11 shows the TNF-a secretion inhibiting effect of the groups A to D. Fig.
Figure 12 shows the cell viability of groups A to D;

Hereinafter, preferred embodiments of the present invention will be described in order to facilitate understanding of the present invention. However, the following examples are provided only for the purpose of easier understanding of the present invention, and the present invention is not limited by the examples.

< Example  1> Preparation of Plant Extract

600 g of each of green tea leaves, green tea seeds, yambaeknisan, corn oil, coriander, bingoji, jiuhei, Tianjin, After filtering (x3 times), the extract was concentrated under reduced pressure in a water bath to obtain methanol extract and used as a sample.

Target plant weight Extraction solvent Extraction temperature Extraction time One Green tea leaves 600g Methanol 50 ℃ 24 hours 2 Green tea seed 600g Methanol 50 ℃ 24 hours 3 A nightstand 600g Methanol 50 ℃ 24 hours 4 Corn oil 600g Methanol 50 ℃ 24 hours 5 Gill pulpit 600g Methanol 50 ℃ 24 hours 6 Bingo 600g Methanol 50 ℃ 24 hours 7 Oil 600g Methanol 50 ℃ 24 hours 8 Tianjin 600g Methanol 50 ℃ 24 hours 9 Cheongpyi 600g Methanol 50 ℃ 24 hours 10 Shake 600g Methanol 50 ℃ 24 hours 11 And seconds 600g Methanol 50 ℃ 24 hours

< Experimental Example  1> Measurement of antioxidative effect of single extract

1-1. Green tea leaves  And green tea seeds DPPH free Radical  Scavenging activity

DPPH free radical scavenging activity was measured by Malterud et al. DPPH solution (45 占 퐂 / ml methanol) was mixed with the extract extracted from Example 1, and the decrease in absorbance at 515 nm was measured at intervals of 30 seconds for 3 minutes. The free radical scavenging activity was expressed on the basis of 100% absorbance reduction of pyrogallol solution (125 μg / ml DMSO).

The DPPH free radical scavenging activities of the methanol extracts of green tea leaves and green tea seeds were 84.8% and 82.4%, respectively, and the inhibitory activities of vitamin C and BHA were 79.8% 76.1%, indicating that they have excellent antioxidative activity (Fig. 1).

1-2. Corn oil, Oil , Bingo DPPH free Radical  Scavenging activity

The DPPH free radical scavenging activities of the extracts of corn oil, oleoresin, bilberry, and methanol were 83.3% and 78.8%, respectively. The inhibitory activities of vitamin C and BHA were 80.5% , 77.5% and 74.4%, respectively, indicating that they have excellent antioxidative activities (FIG. 2).

1-3. Gill pulpit , And seconds , At the door DPPH free Radical  Scavenging activity

The DPPH free radical scavenging activity of methanol extracts of G. crassifolia, B. japonica and A. basalis showed 85.6% and 81.9% inhibition activity of the positive control, vitamin C and BHA, respectively, and 28.4% , 74.4%, and 76.2%, respectively, indicating that the supergene and yamancom had excellent antioxidative activities (Fig. 3).

1-4. Tianjin, Shake , Cheongpy DPPH free Radical  Scavenging activity

The DPPH free radical scavenging activity of methanol extracts from cinnabar, shaved and chewy plants were 86.4% and 83.2%, respectively, and positive control, vitamins C and BHA were 83.2%, 28.7% , 80.0%, and 37.6%, respectively, indicating that the anti-oxidation activity exhibited excellent anti-oxidation activity (Fig. 4).

1-5. Superoxide  Scavenging activity superoxide quenching activity )

Riboflavin is decomposed in light of about 5500 lux, producing singlet oxygen and superoxide anion. At this time, when EDTA is added, soluble chelate is formed with Cu and Fe in the aqueous solution to inhibit the change of riboflavin by the metal. The singlet oxygen removes methionine and obtains pure superoxide radicals. The superoxide thus produced is reduced to NBT (Nitro Blue tetrazolin - itself absorbs light at 260 nm in yellow) to produce blue formazane (absorbance = 560 nm), which is a reducing material of NBT, An experiment to suppress color change is an experiment of superoxide quenching activity.

The superoxide scavenging activities of methanol extracts of corn oil, oleaginous oil, linseed oil, flatulence, phyllotaxis, cheongyubi, panchromatic vein, and manure were similar to those of DPPH free radical scavenging activity (Fig. 5 ).

Through the experiments of 1-1 to 1-5, eight kinds of plant extracts composed of green tea leaves, green tea seeds, marine oil, jute oil, bingo, one side, .

< Example  2> Preparation of mixed extract

Among the eight plant extracts proven to have antioxidative effect in Experimental Example 1, the green tea leaf extract, the green tea seed extract, the crude oil extract, the chrysanthemum extract, the yambi extract and the marine oil extract were mixed at a weight ratio of 1: 1, , And C were prepared. Further, the mixed extracts of Groups A, B, and C were mixed again to prepare an extract of Group D.

Target plant Weight ratio Group A Green tea leaf extract Green tea seed extract 1: 1 Group B Oil extract Chrysanthemum extract 1: 1 Group C A nightmarish extract Corn oil extract 1: 1 Group D A mixture of Groups A, B and C

< Experimental Example  2> Antioxidative Effect of Mixed Extracts

2-1. DPPH free Radical  Scavenging activity

The DPPH free radical scavenging activity of the Group A to D mixed extracts showed excellent antioxidative effects in the group A to D mixed extracts as well as the activity of the positive control groups of vitamin C and BHA (86.2% and 80.7%, respectively) The whole mixture of Group D showed about 80%, showing very excellent effect (FIG. 6).

2-2. Superoxide  Scavenging activity superoxide quenching activity )

The superoxide scavenging activities of the mixed extracts of Groups A and D showed excellent antioxidative effects in the group A to D mixed extracts as well as the activities of the control groups of vitamin C and BHA (86.9% and 72.0%, respectively) The total mixture showed about 82.2%, showing very excellent effect (FIG. 8).

2-3. Xanthin Oxidase NBT assay

The Xanthine Oxidase NBT assay was performed to confirm the inhibitory effect of the mixed extract obtained in Example 2 on Xanthine oxidase activity. Xanthin is decomposed by Xanthin oxidase to produce uric acid + O ₂ ^- . O ₂ ^- reduces NBT to produce blue formazane (blue), which is a reducing agent of NBT, at which time the Xanthin oxidase NBT test inhibits the change in color.

Xanthine originated superoxide quenching activities of the mixed extracts of Groups A and D, as well as the activity of vitamin C and BHA (representing 102.8% and 81.5%, respectively) in the positive control groups, were also excellent And showed an excellent antioxidative effect, with about 101.6% of the total mixture of Group D (FIG. 8).

< Experimental Example  3> Whitening effect test of mixed extract

 3-1. Tyrosinase  Measurement of inhibitory activity

After adding 25 μl of 10 mM L-tyrosine (Sigma, USA) and 10 μl of the mixed extract of Example 2 to 150 μl of 0.1 M phosphate buffer (pH 6.5), 10 μl of tyrosinase (50 units / ml, After incubation at 36 ° C for 10 minutes, absorbance was measured at 490 nm. Kojic acid (Sigma, USA) was used as a positive control.

As a result of measuring the tyrosinase inhibitory effect of each group, group C, which is a mixed extract of yamamun and corn oil, exhibited a relatively excellent tyrosinase inhibitory effect, and the whole mixture, D, also showed excellent tyrosinase inhibitory effect (Fig. 9) .

3-2. Melanin formation inhibitory effect

The melanocytes (HEMn) were cultured and inoculated into 6 wells at 5 × 10 ⁴ cells / well. The extracts of Example 2 were treated in each well and cultured for 48 hours. After incubation, the cells were washed twice with phosphate buffered saline (PBS), and treated with trypsin to recover the cells. 1N NaOH (10% DMSO) was added to the recovered cells and allowed to stand in a 60 ° C thermostat for 1 hour to dissolve the melanin, and the absorbance was measured at 490 nm.

As a result of inhibition of melanin production, group D, which is the total mixture, showed the most excellent effect, and group C, which had the highest tyrosinase inhibitory effect, also had a significant concentration-dependent inhibitory effect on melanogenesis (FIG. 10) . This result suggests that it can be used as a natural whitening raw material due to its effect close to kojic acid as a control.

< Experimental Example  4> anti-inflammatory effect TNF -α secretion) measurement

To investigate the effect of TNF-α on the inflammatory cytokine secretion, RAW 264.7 macrophages were treated with LPS (10 μg / ml) alone or LPS and Group A to D mixed extracts at a concentration of 200 μg / TNF-α production was examined. As a result, it was found that all the extracts of Groups A to D at 200 ug / mL inhibited the secretion of TNF-α and thus had excellent anti-inflammatory action (FIG. 11).

< Experimental Example  5> Safety Test for Cytotoxicity ( MTT assay )

Toxicity measurements of the cells of Groups A to D were determined by 5- (3-carboxymethyl-oxyphenyl) -2H-tethra-zolium inner salt (MTS) assay. It measures the conversion of MTS to formazan by mitochondrial dehydrogenase. 10 ⁵ cells / well of RAW 264.7 cells were seeded in a 96-well plate and cultured for 18 hours. The combined extracts of Groups A and D were treated with 1 ug / ml, 10 ug / ml and 100 ug / ml, followed by incubation for 24 hours, and cytotoxicity was measured. Positive control kojic acid (Sigma, USA) was used.

The cell viability of the RAW 264.7 cell group A to D mixed extracts was measured and found to be not toxic at all concentrations of the mixed extracts of Groups A to D and was lower than that of positive control kojic acid It was confirmed that the safety was very excellent (Fig. 12).

< Formulation example  1> Production of lotion

Table 3 shows the constitutional constituents of the lotion containing the mixed extract of group C (mixed extract of gazebo and mountainous oil) prepared in Example 2 above. The preparation of the lotion was carried out according to conventional methods in the art.

Raw material Content (% by weight) One Mixed extract of group C 5.0 2 glycerin 3.0 3 Butylene glycol 2.0 4 Propylene glycol 2.0 5 Polyoxyethylene (60) Hardened castor oil 1.0 6 ethanol 10.0 7 Triethanolamine 0.1 8 antiseptic a very small amount 9 Pigment a very small amount 10 Spices a very small amount 11 Purified water Balance 100

< Formulation example  2> Manufacture of Lotion

Table 4 shows the ingredient composition of the lotion containing the mixed extract of group C prepared in Example 2 (the mixed extract of the yam door and the marine oil). The lotion was prepared according to conventional methods in the art.

Raw material Content (% by weight) One Plant mixture extract 5.0 2 Sitosterol 1.7 3 Polyglyceryl 2-oleate 1.5 4 Ceramide 0.7 5 Setares-4 1.2 6 cholesterol 1.5 7 Dicetyl phosphate 0.4 8 glycerin 5.0 9 Sunflower oil 10 10 Carboxyvinyl polymer 0.2 11 Xanthan gum 0.3 12 Triethanolamine 0.2 13 antiseptic a very small amount 14 Spices a very small amount 15 Purified water Balance 100

< Formulation example  3> Manufacture of cream

Table 5 shows the composition of the cream containing the mixed extract of group C (mixed extract of yamamatsu and marinated oil) prepared in Example 2 above. The cream was prepared according to conventional methods in the art.

Raw material Content (% by weight) One Plant mixture extract 5.0 2 Sitosterol 4.0 3 Polyglyceryl 2-oleate 3.0 4 Ceramide 0.7 5 Setares-4 2.0 6 cholesterol 3.0 7 Dicetyl phosphate 5.0 8 glycerin 5.0 9 Sunflower oil 22 10 Carboxyvinyl polymer 0.5 11 Triethanolamine 0.5 12 antiseptic a very small amount 13 Spices a very small amount 14 Purified water Balance 100

Claims (9)

An antioxidant, an antioxidant, an antioxidant, an antioxidant, an antioxidant, an antioxidant, an antioxidant, an antioxidant.
delete The composition according to claim 1, further comprising green tea leaves and green tea seed extract.
The herbal cosmetic composition for antioxidant, whitening and anti-inflammation according to claim 3, further comprising a crude oil extract.
delete The herbal cosmetic composition for antioxidant, whitening and anti-inflammation according to claim 4, further comprising bingo extract.
The herbal cosmetic composition for antioxidant, whitening and anti-inflammation according to claim 4, further comprising a monocotyledonous extract.
delete The method according to any one of claims 1, 3, 4, 6, and 7,
The composition may be in the form of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a water lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisture cream, a hand cream, a foundation, , A cleansing lotion, a cleansing cream, a body lotion, and a body cleanser.

Images