KR101099664B1 - Composition for prevention of hair loss or hair restoration containing extracts of the sepal of strawberry - Google Patents

Abstract

PURPOSE: A cosmetic composition containing strawberry sepal extract is provided to promote hair growth and to prevent alopecia without side effect. CONSTITUTION: A cosmetic composition for promoting hair growth or preventing male pattern alopecia contains 0.1-50 wt% of strawberry sepal extract. The strawberry sepal extract is prepared using water, alcohol of 1-5 carbon atoms or diol of 2-5 carbon atoms. The cosmetic composition further contains strawberry flesh extract.

Description

Composition for prevention of hair loss or hair restoration containing extracts of the sepal of strawberry}

The present invention relates to a cosmetic composition for preventing hair loss or promoting hair growth. More specifically, the present invention relates to a composition containing a strawberry calyx extract, that is, a strawberry faucet extract, to prevent hair loss or promote hair growth.

The human hair is about 10,000 to 150,000 pieces, each hair has a different hair cycle to grow, degenerative, resting periods are generated, grown and dropped out. As a result, about 15% of the hair is placed in the resting and degenerative stages, so that on average 50-100 hairs fall out and regenerate normally.

In general, alopecia refers to a period in which the growth phase of the hair cycle is shortened due to various causes, so that the ratio of degenerative and resting hair increases relatively, so that the number of hair dropouts is higher than the average value. The major causes of alopecia include the theory of genetic androgen hormones, nutritional disorders caused by regional blood flow disorders, autonomic nervous system disorders, autoimmune disorders, and endocrine disorders. In particular, many studies on androgenic alopecia, which is thought to be the main cause of testosterone, have been conducted at home and abroad, but there is no clear cause for hair loss.

The mechanism of action of androgenetic alopecia is as follows. Testosterone, one of the male hormones, is converted to dehydrotestosterone, an active male hormone, by 5 alpha-reductase. The abnormal overaction of this hormone is a substance that causes male hair loss. Therefore, inhibiting the activity of 5-alpha-reductase will effectively prevent and treat male-type hair loss.

In recent years, the population that causes abnormal hair loss due to changes in diet, lifestyle changes, and stress caused by social environment is gradually increasing. In particular, there is a growing population of young women, the elderly and women's baldness.

Minoxidil is a drug for promoting hair growth. Minoxidil was used as an antihypertensive agent. Minoxidil is known to promote hair growth by increasing blood flow in the scalp where the hair follicles are located. However, when minoxidil is used as a hair growth promoter for a long time, its effect may be halved, and side effects due to chemical vasodilation action have been reported as asthenia, increased sebum. In addition, hair loss treatment or hair growth based on chemical drugs has a problem that can cause new side effects that are not known.

Therefore, there is a need for the development of a composition having a bio-friendly hair loss prevention or hair growth promoting effect that is not dependent on chemical drugs.

It is an object of the present invention to provide a 5 alpha-reductase inhibitor which contains a strawberry faucet extract derived from natural products and exhibits 5 alpha-reductase inhibitory activity.
It is another object of the present invention to provide a cosmetic composition for preventing hair loss or promoting hair growth containing a strawberry faucet extract having the 5 alpha-reductase inhibitory activity.
Another object of the present invention is to provide a composition for preventing hair loss or promoting hair growth that has no side effect of preventing hair loss or promoting hair growth.

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The present invention relates to a 5 alpha-reductase inhibitor containing strawberry tap extract.
The present invention also relates to a cosmetic composition for preventing hair loss or promoting hair growth containing the strawberry stem extract.
In the present invention, the composition may be particularly for improving male alopecia.
The 5 alpha-reductase inhibitors of the present invention may further contain strawberry pulp extract.
In one embodiment, the composition for preventing hair loss or promoting hair growth may contain 0.1-50% by weight of the strawberry nipple extract in the total composition.

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In one embodiment, the strawberry nipple extract can be extracted from the strawberry nipple using one or more extraction solvents selected from the group consisting of water, monoalcohols of 1-5 carbon atoms and diols of 2-5 carbon atoms.

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The present invention provides a composition for preventing hair loss or promoting hair growth having a hair loss prevention or hair growth promoting effect derived from a natural product. In addition, the present invention provides a composition for preventing hair loss or hair growth which has no side effect of preventing hair loss or promoting hair growth.

1 relates to the inhibition experiment of 5 alpha reductase. The lower band represents testosterone and the upper band represents dihydrotestosterone. In Figure 1 column 1 extract 0.1%, column 2 extract 0.5%, column 3 extract 1%, column 4 extract 2%, column 5 shows the radioactive results of the control. Higher treatment concentrations of the extract indicate that production of dihydrotestosterone is inhibited.
Figure 2 shows the free radical scavenging activity according to the concentration of the strawberry spigot extract, the X-axis shows the concentration (%) of the strawberry spigot extract, the Y-axis shows the free radical scavenging activity (%).
Figure 3 shows the cell activity according to the extraction solvent (DW: purified water, EtOH: ethanol, 1,2PD: 1,2-pentanediol, 1,3BG: 1,3-butylene glycol) and the concentration of the strawberry nipple extract.

The 5 alpha-reductase inhibitors of the present invention and the composition for preventing hair loss or promoting hair growth contain strawberry calyx extract, that is, strawberry faucet extract.

Strawberry Calyx Extract

Strawberry calyx extract refers to an extract, ie, a strawberry faucet extract, manufactured using only a strawberry faucet portion from which the pulp portion of the strawberry is removed. In the present specification, 'strawberry nipple' means a portion excluding the pulp portion of the strawberry when the strawberry is purchased, and is derived from the calyx of the strawberry flower. Strawberry faucet extract may be contained in 0.1-50% by weight of the composition for preventing hair loss or promoting hair growth. Preferably, the strawberry faucet extract may be contained in 0.1-25% by weight, more preferably 0.1-5% by weight in the composition for preventing hair loss or promoting hair growth.

Strawberry faucet extracts can be prepared using conventional extraction methods. For example, although the extraction method using an organic solvent, the hot water extraction method, the extraction method using supercritical carbon dioxide, etc. can be used, it is not limited to these. Preferably, the method may be extracted by an extraction method using an organic solvent or a hydrothermal extraction method.

The extraction solvent is not particularly limited, but for example, one or more extraction solvents selected from the group consisting of water, mono alcohols having 1 to 5 carbon atoms and diols having 2 to 5 carbon atoms can be used. For example, one or more extraction solvents selected from the group consisting of water, alcohols having 1-3 carbon atoms, 1,3-butylene glycols having 4-5 carbon atoms, and 1,2-pentanediol can be used. Preferably, one or more solvents selected from the group consisting of water, ethanol, 1,3-butylene glycol and 1,2-pentanediol can be used.

The extraction method may be prepared by adding the extraction solvent to the strawberry nipple itself or the pulverized product obtained after drying the strawberry nipple and extracting at 40-110 ° C. for 1-10 hours, but is not limited thereto.

Strawberry faucet extracts can inhibit hair loss or promote hair growth by inhibiting the activity and production of 5 alpha-reductase that promotes the production of dihydrotestosterone. In addition, free radicals can be overproduced with free radicals against external stimuli to destroy the skin's moisturizing layer, resulting in dry skin and an immune response sensitive to external stimuli. Therefore, free radical scavenging activity is required for hair loss prevention or hair growth promoting effect. Strawberry faucet extract may have a free radical scavenging activity to help prevent hair loss or promote hair growth. In addition, strawberry faucet extract is not derived from chemicals but from natural products, and is non-irritating and cytotoxic to the skin including the scalp.

The 5 alpha-reductase inhibitors of the present invention and the composition for preventing hair loss or promoting hair growth contain strawberry stem extract. Strawberry faucet extract may also be contained in liquid, solid or mixed form of liquid and solid. Although the content of the strawberry nipple extract in the composition for preventing hair loss or promoting hair growth is not particularly limited, it is preferably contained in 1-50% by weight.

The 5 alpha-reductase inhibitors of the present invention and the composition for preventing hair loss or promoting hair growth may further contain an extract of the strawberry pulp portion, in addition to the extract of the strawberry stem. The extract of the strawberry pulp portion may be extracted using the same extraction solvent and extraction method as the strawberry nipple extract, but is not limited thereto. Extract of the strawberry pulp portion may be included in 0.1-20% by weight of the composition.

The The composition may comprise, in addition to the strawberry nipple extract, a conventional solvent which can be used in the preparation of the composition as the balance of the composition. The solvent may include water, but is not limited thereto.

Hair loss prevention or hair growth promoting composition of the present invention can be used as a pharmaceutical composition or cosmetic composition for the purpose of hair loss prevention or hair growth promotion. When used as a pharmaceutical composition, it can be used in various formulations such as topical application such as ointment, cream, lotion, liquid, spray, etc. in use. Preferably, it is effective to formulate into topical application preparations such as ointments, lotions, sprays and the like and apply them directly to the hair loss site. The pharmaceutical composition can be used for the prevention or treatment of baldness or alopecia. In addition, when used as a cosmetic composition, it can be formulated into any formulation that can be applied to conventional scalp, such as shampoos, hair conditioners, hair lotions, liquid hair growth promoter types, and the like.

The composition for preventing hair loss or promoting hair growth of the present invention may further include one or more known herbal ingredients known to have hair loss preventing or hair growth promoting effects as necessary. For example, it may be in the form of an oil or extract extracted from baekbaek, baekbaek, ginseng, licorice, cloves, safflower, dermis, cheonho, green tea, dried branches, dermis, flaxseed, pomegranate, cornus, wolfberry, and the like. When the herbal ingredient is included in the hair loss preventing or hair growth promoting composition of the present invention, the content is preferably 1-50% by weight.

The composition for preventing hair loss or promoting hair growth of the present invention may include conventional mixing ingredients such as hinokithiol, capsicum tink, tocopherol acetate, nico acid amide, nico acid benzine, pyroxtonolamine, salicylic acid, and l-mentone.

Hereinafter, the configuration and operation of the present invention through the preferred embodiment of the present invention will be described in more detail. However, this is presented as a preferred example of the present invention and in no sense can be construed as limiting the present invention.

Details that are not described herein will be omitted since those skilled in the art can sufficiently infer technically.

Example  1: Preparation of strawberry calyx extract (strawberry extract)

After collecting the strawberry nipple material, it was washed with a detergent to remove foreign substances. After the strawberry nipple material was dried, the prepared dried material was ground using a grinder. 7 g of the strawberry pulverized dry powder was put in 250 g of purified water, heated at 80 ° C. for 4 hours, and cooled. Two days later, the extract obtained was filtered and concentrated to obtain a strawberry faucet extract.

Example  2: Preparation of Strawberry Tap Extract

The same method as in Example 1 was performed except that 250 g of 95% ethanol was used instead of purified water in Example 1 to obtain a strawberry stem extract.

Example  3: Preparation of Strawberry Tap Extract

Except that 250 g of 1,3-butylene glycol aqueous solution in Example 1 (volume ratio of purified water: 1.3-butylene glycol is 7: 3) was carried out in the same manner as in Example 1 to obtain a strawberry faucet extract .

Example  4: Preparation of Strawberry Tap Extract

Except that 250g of 1,2-pentanediol aqueous solution (volume ratio of purified water: 1,2-pentanediol is 7: 3) in Example 1 was carried out in the same manner as in Example 1 to obtain a strawberry faucet extract .

Experimental Example  1: 5 Alpha- of Strawberry Calyx Extract (Strawberry Tap Extract) Reductase  Activity inhibition assessment

5 alpha-reductase activity used in the evaluation of inhibition of 5 alpha-reductase activity was used an enzyme produced by epidermal fibroblasts. Fibroblasts isolated from skin cells were seeded at 1 x 10 ⁴ per microplate hole and incubated at 37 ° C for 24 hours. In each hole, testosterone radiolabeled with ³ H was added at 0.1 mCiC, and the extract prepared in Example 1 was added at a concentration of 0.1-2%. The fibroblasts were cultured at 37 ° C. to determine whether the fibroblasts used testosterone. After culturing for 24 hours, fibroblast supernatant was obtained, and steroid was obtained with 1 ml of 1: 1 (volume ratio) extraction solvent of ethyl acetate-cyclohexane. The obtained steroid was placed on a thin layer chromatography plate and developed with a developing solvent (mixture of chloroform / methanol, 98/1, volume ratio). The conversion of testosterone to dihydrotestosterone was calculated by measuring the radioactivity of the points corresponding to testosterone and dihydrotestosterone using a densitometer.

The conversion rate of testosterone to dihydrotestosterone was calculated using the same method as the comparative example, except that purified water was used without using the extract prepared in Examples 1-4.

Using this, the activity inhibitory ability of 5 alpha-reductase was calculated by the following formula (1).

<Equation 1>

Inhibitory Activity (%) = (A-B) / A x 100

(Wherein A represents the conversion rate of testosterone to dihydrotestosterone when no extract is added, and B represents the conversion rate of testosterone to dihydrotestosterone when the extract is added).

The results are shown in Table 1 and FIG. 1.

Concentration of extract of Example 1 Comparative example
12.5 0.1% 0.5% One% 2% Inhibition
(%) 15.3 21.2 56.0 62.2 Concentration of extract of Example 2 0.1% 0.5% One% 2% Inhibition
(%) 11.1 15.8 34.6 48.5 Concentration of extract of Example 3 0.1% 0.5% One% 2% Inhibition
(%) 18.7 26.6 48.3 55.6 Concentration of extract of Example 4. 0.1% 0.5% One% 2% Inhibition
(%) 13.3 17.9 30.2 42.2

As shown in Table 1 and Figure 1, it can be seen that the activity inhibitory activity of 5 alpha-reductase is higher than that of the comparative example when the strawberry calyx extract is added. This indicates that the strawberry calyx extract has an anti-hair loss or hair promoting effect.

Experimental Example  2: Freedom of Strawberry Calyx Extract (Strawberry Tap Extract) Radical  Scavenging activity assessment

1 mM DPPH (1,1-diphenyl-2-picryl hydrazyl, sigma D9132-1G, USA) is known to generate free radicals in ethanol. In 0.75 ml of 0.1 mM DPPH solution prepared with ethanol, the extract prepared in Example 1 was diluted to 0-100 wt%, 0.15 ml was added, and 0.6 ml of ethanol was added. The mixture was stirred vigorously for 10 seconds, stored in a cool dark place for 30 minutes, and the absorbance was measured at 520 nm using an ELISA reader (Thermo, Multiskan EX).

As a negative control, 0.15 ml of purified water was added instead of the extract prepared in Example 1, and the absorbance was measured by the same method. As a positive control, 25 μg / ml of ascorbic acid was added instead of the extract prepared in Example 1, and the absorbance was measured by the same method. Free radical scavenging activity can be evaluated by the following formula 2, the results are shown in Table 2 and FIG.

<Equation 2>

% Free radical scavenging activity = 100-((absorbance of experimental group) / (absorbance of negative control)) x 100

% Concentration of extract of Example 1 Positive control 0.04375 0.0875 0.175 0.35 0.7 1.4 2.8 % Free radical scavenging activity 1.2 12.7 7.7 45.3 44.7 41.6 42.8 49.6

As shown in Table 2, the strawberry tap extract of the present invention shows similar results to ascorbic acid, which is known to have antioxidant activity by removing free radical scavenging activity. Therefore, strawberry teats were found to have good antioxidant activity.

Experimental Example  3: Cytotoxicity Test of Strawberry Calyx Extract (Strawberry Tap Extract)

Make sure that the strawberry faucet extract is nonirritating and nontoxic to the scalp. Human keratinocytes HaCat (ACTT, CLS 300493, USA) were counted using a hemacytometer equally at 5 x 10 ³ cells / well in 96-well plates, aliquoted, and then aliquoted at 37 ° C., 5% Incubated for 24 hours in carbon dioxide conditions. After culturing for 24 hours, the obtained cell culture solution was removed, and 100 μl of the extract obtained in Example 1-4 was added to each well at 0.1, 0.5, 1 and 2 (weight / volume)% in DMEM medium at 37 ° C., 5 Incubated for 24 hours at% carbon dioxide conditions. Thereafter, 10 μl of WST, a cell proliferation reagent, was added to each well and further incubated for 4 hours under the same conditions. Thereafter, the absorbance was measured at 420 nm with an ELISA reader (Thermo, Multiskan EX).

In the above, absorbance was measured by the same method except that the same amount of purified water was used instead of the extract. The absorbance was measured using the same amount of DMEM (10% FBS) instead of the extract as a positive control and the same amount of DMSO instead of the extract as the negative control.

The degree of cytotoxicity can be expressed by Equation 3 below, and the results are shown in Table 3 and FIG. 3.

<Equation 3>

% Cytotoxicity = (absorbance of experimental group / absorbance of positive control group) X 100

Concentration of Extracts of Examples 1-4 Negative control
0.1% 0.5% One% 2% Cytotoxicity (%) 111.66 101.86 102.68 103.62 22.31

As shown in Table 3 it can be seen that the cytotoxicity is significantly lower than the negative control.

Experimental Example  4: of strawberry calyx extract (strawberry extract) Bacteriostatic  Experiment

In order to check the number of viable bacteria in the extract, the extract prepared in Example 1-4 was left at 25 ° C. for 30 days, and then the number of viable bacteria was checked. 0.1 ml of the extracted extract was plated in LB agar medium and incubated for 48 hours in an incubator at 37 ° C. Culture was confirmed the presence of bacteria. No bacterial growth was confirmed in any of the extracts.

Formulation example  1: prevent hair loss or promote hair Hair lotion

The hair lotion was prepared by the conventional method using the component of Table 4 below.

ingredient Content (% by weight) Extract of Example 1 1.0 Cetostearyl alcohol 2.0 Stearyl Triethyl Ammonium Chloride 2.0 Hydroxyethyl cellulose 0.5 Pyrotonolamine 0.1 Spices 0.5 Purified water Balance system 100.0

Formulation example  2: hair tonic that prevents hair loss or promotes hair

The hair tonic was prepared by the conventional method using the component of Table 5 below.

ingredient Content (% by weight) Extract of Example 2 1.0 ethanol 55.0 Castor oil 5.0 glycerin 3.0 Pyrotonamine 0.1 Spices, pigments 0.01 Purified water Balance system 100.0

Claims (4)

Cosmetic composition for preventing male hair loss or promoting hair growth in male hair loss, containing strawberry faucet extract, which prevents male hair loss or promotes hair growth in male hair loss by inhibiting 5 alpha-reductase activity by the strawberry faucet extract. .
The cosmetic composition for preventing hair loss or promoting hair growth in male hair loss according to claim 1, wherein the strawberry stem extract is contained in an amount of 0.1-50% by weight in the total composition.
The method of claim 1, wherein the strawberry faucet extract is characterized in that the extract from the strawberry faucet using at least one extraction solvent selected from the group consisting of water, C1-5 alcohols and C2-5 diols, Cosmetic composition for preventing hair loss or promoting hair growth in male hair loss.
The cosmetic composition for preventing hair loss or promoting hair growth in male hair loss, according to claim 1, wherein the composition further comprises a strawberry pulp extract.

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