KR102264642B1 - 단백질 스크리닝 및 검출 방법 - Google Patents

단백질 스크리닝 및 검출 방법 Download PDF

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KR102264642B1
KR102264642B1 KR1020197014386A KR20197014386A KR102264642B1 KR 102264642 B1 KR102264642 B1 KR 102264642B1 KR 1020197014386 A KR1020197014386 A KR 1020197014386A KR 20197014386 A KR20197014386 A KR 20197014386A KR 102264642 B1 KR102264642 B1 KR 102264642B1
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tag
library
polypeptide
detection tag
nucleic acid
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KR20190104312A (ko
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마르쿠스 제거
파스칼 에글로프
이반 침머만
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유니베르시태트 취리히
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/22—Immunoglobulins specific features characterized by taxonomic origin from camelids, e.g. camel, llama or dromedary
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/22—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a Strep-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/24—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a MBP (maltose binding protein)-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/41—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a Myc-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/43—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a FLAG-tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/119—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being proteinic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/185—Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
KR1020197014386A 2016-10-31 2017-10-30 단백질 스크리닝 및 검출 방법 Active KR102264642B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP16196571.0 2016-10-31
EP16196571 2016-10-31
PCT/EP2017/077816 WO2018078167A1 (en) 2016-10-31 2017-10-30 Protein screening and detection method

Publications (2)

Publication Number Publication Date
KR20190104312A KR20190104312A (ko) 2019-09-09
KR102264642B1 true KR102264642B1 (ko) 2021-06-15

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Country Link
US (2) US12054708B2 (https=)
EP (2) EP4123022A1 (https=)
JP (1) JP7185929B2 (https=)
KR (1) KR102264642B1 (https=)
CN (1) CN110225973B (https=)
AU (1) AU2017351810B2 (https=)
CA (1) CA3041406A1 (https=)
DK (1) DK3532612T3 (https=)
EA (1) EA201991019A1 (https=)
ES (1) ES2926548T3 (https=)
HU (1) HUE059755T2 (https=)
IL (1) IL266270B2 (https=)
PL (1) PL3532612T3 (https=)
PT (1) PT3532612T (https=)
SG (1) SG11201903550TA (https=)
WO (1) WO2018078167A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012024289A2 (en) 2010-08-16 2012-02-23 Smith & Nephew Inc. Systems and methods for altering the surface of a bone
JP6683867B1 (ja) * 2019-07-22 2020-04-22 植田　充美 モノクローナル抗体の酵母による製造方法およびスクリーニング方法
WO2024026026A1 (en) * 2022-07-27 2024-02-01 Trustees Of Tufts College High throughput in vivo screening of lipid nanoparticles

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997007132A1 (en) * 1995-08-15 1997-02-27 Commonwealth Scientific And Industrial Research Organisation Epitope tagging system
JP2007502417A (ja) 2003-08-11 2007-02-08 モノグラム バイオサイエンシズ，インコーポレーテッド 分子複合体の検出および特徴づけ
KR100810699B1 (ko) 1998-11-20 2008-03-07 커먼웰쓰 사이언티픽 앤드 인더스트리얼 리서치 오가니제이션 항원 유전자 서열의 동정 방법
JP2010538618A (ja) 2007-09-11 2010-12-16 シー レーン バイオテクノロジーズ， エルエルシー ドナー特異的抗体ライブラリー

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60029572T2 (de) 1999-03-23 2007-07-26 Biovation Ltd. Isolierung und analyse von proteinen
US20090233806A1 (en) * 1999-07-06 2009-09-17 Carr Francis J Protein isolation and analysis
GB0217402D0 (en) * 2002-07-26 2002-09-04 Roslin Inst Edinburgh Multi-reporter gene model for toxicological screening
JP2004329040A (ja) * 2003-05-01 2004-11-25 Daiichi Fine Chemical Co Ltd ポリセラーゼ−ｉ及びその利用
US20060073506A1 (en) 2004-09-17 2006-04-06 Affymetrix, Inc. Methods for identifying biological samples
EP2024393A2 (en) 2006-05-15 2009-02-18 Sea Lane Biotechnologies,llc. Neutralizing antibodies to influenza viruses
EP2436766A1 (en) * 2010-09-29 2012-04-04 Deutsches Krebsforschungszentrum Means and methods for improved protein interaction screening
WO2014139130A1 (en) * 2013-03-14 2014-09-18 Adagene Inc. An integrated system for library construction, affinity binder screening and expression thereof
US11339434B2 (en) * 2016-07-29 2022-05-24 The Regents Of The University Of California Methods for determining gene functions
CN108802162B (zh) * 2016-11-25 2020-08-18 北京毅新博创生物科技有限公司 通过内部标准物质谱检测微生物的方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997007132A1 (en) * 1995-08-15 1997-02-27 Commonwealth Scientific And Industrial Research Organisation Epitope tagging system
KR100810699B1 (ko) 1998-11-20 2008-03-07 커먼웰쓰 사이언티픽 앤드 인더스트리얼 리서치 오가니제이션 항원 유전자 서열의 동정 방법
JP2007502417A (ja) 2003-08-11 2007-02-08 モノグラム バイオサイエンシズ，インコーポレーテッド 分子複合体の検出および特徴づけ
JP2010538618A (ja) 2007-09-11 2010-12-16 シー レーン バイオテクノロジーズ， エルエルシー ドナー特異的抗体ライブラリー

Also Published As

Publication number Publication date
KR20190104312A (ko) 2019-09-09
EP4123022A1 (en) 2023-01-25
IL266270A (en) 2019-06-30
DK3532612T3 (da) 2022-09-19
EP3532612A1 (en) 2019-09-04
PT3532612T (pt) 2022-07-26
JP2020502493A (ja) 2020-01-23
US20240327825A1 (en) 2024-10-03
IL266270B1 (en) 2024-09-01
JP7185929B2 (ja) 2022-12-08
AU2017351810A1 (en) 2019-05-16
AU2017351810B2 (en) 2023-07-20
US12054708B2 (en) 2024-08-06
ES2926548T3 (es) 2022-10-26
US20190276819A1 (en) 2019-09-12
IL266270B2 (en) 2025-01-01
EP3532612B1 (en) 2022-06-29
EA201991019A1 (ru) 2020-01-22
CN110225973A (zh) 2019-09-10
CA3041406A1 (en) 2018-05-03
HUE059755T2 (hu) 2022-12-28
CN110225973B (zh) 2024-02-13
WO2018078167A1 (en) 2018-05-03
SG11201903550TA (en) 2019-05-30
PL3532612T3 (pl) 2022-10-31

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