KR102258773B1 - An animal feed form with poultry productivity improvement - Google Patents

Abstract

The present invention relates to animal feed for improving poultry productivity.

Description

Feed composition for improving the productivity of poultry {AN ANIMAL FEED FORM WITH POULTRY PRODUCTIVITY IMPROVEMENT}

The present invention relates to the effect of improving the poultry productivity of a feed composition using mugwort extract.

In order to improve the productivity of livestock in the prior art, various additives for promoting intestinal development such as feed additives (probiotics, etc.) and antibiotics for promoting growth have been continuously fed. In particular, growth-promoting antibiotics promote vitamin synthesis by adjusting the intestinal microflora and increase nutrient utilization by improving the gastrointestinal mucosa (Groschke, 1950; Thayer et al., 1950). In addition, it inhibits the colonization and proliferation of microorganisms in the gastrointestinal tract to improve the utilization of nutrients in feed (Dibner and Richards, 2005), and suppresses the proliferation of Clostridium perfringens , the causative agent of necrotizing enterocolitis, to suppress the induction of intestinal inflammation (Lev and Forbs, 1959). ). In addition, various effects such as reducing intestinal mucosa damage, abnormal nutrient absorption, growth retardation, etc. by inhibiting microbial urease, and increasing the nutrient absorption rate by promoting the differentiation of small intestine mucosal cells and villi (Parker, 1990), are the most commonly used. has been

Republic of Korea Patent No. 1994-0000065 discloses the effect of using antibiotics in animal feed on livestock health promotion and weight gain. Korean Patent No. 1995-0009944 discloses an animal feed or beverage additive containing an antibiotic mixture of gentamicin, lincomycin or clindamycin for prevention and treatment of infectious diseases and weight gain of carnivores. Republic of Korea Patent Registration No. 921620 claims a feed additive for animals for growth promotion, including yacon outcrops, yacon leaves, yacon stems, sulfur and loess powder. Korean Patent No. 906838 claims a method for producing pelletized animal feed using an enzyme solution for animals. Republic of Korea Patent No. 1008463 discloses a veterinary composition for preventing and treating digestive diseases of pigs using an extract of baeja baeja having antibacterial and antiviral activity.

In the case of antibiotics used for the treatment of bacterial diseases, when used in animals, the wound healing speed is fast, the price is low, and there are advantages such as promoting growth.

However, according to the 'Antibiotic Sales Performance by Year' published by the Korea Veterinary Science and Quarantine Service from 2001 to 2004 and the data analyzing foreign data, in the 'Policy Report on the Misuse and Abuse of Antibiotics in Korea's Livestock Industry', 1,500 tons of livestock production is 16 times that of Denmark (94 tons per year), which is 1.2 times our livestock production.” This figure claimed that “Korea’s use of antibiotics relative to production is the highest in the world”. In fact, according to livestock, the average number of antibiotics used from 2001 to 2003 was pigs (871,741 kg), chickens (359,75 kg), aquatic products (192,699 kg), and cattle (109,500 kg).

Even if the use of these antibiotics is not a big problem in the beginning, there is a problem that resistance occurs due to an immune response if it is continuously used. That is, due to this, there is a problem in that a larger amount must be used in the future, but the medicinal effect is exerted. In addition, in the case of humans, even if they have never taken antibiotics, it is easy to think that the same problem will eventually occur if they eat meat grown while eating antibiotics.

In order to solve this problem, the government regulated the amount of use in compounded feed. As a result, the productivity of livestock decreased (feed intake decreased, mortality increased, flock uniformity decreased, weight gain decreased, feed efficiency decreased), environmental pollution increased, and disease incidence increased. From an economic standpoint, the production cost is expected to increase by more than KRW 1 trillion per year due to regulations on the use of antibiotics (Veterinary Medicine Association, 2010). In particular, in the case of poultry, necrotizing enterocolitis (a bacterial disease caused by Welch bacteria, which causes necrosis in the intestines and damages the decrease in growth rate and increase in pessa rates), coccidiosis (parasitic protozoa, Eimeria genus Coccidia protozoa, parasitizes in the intestinal tract of chickens) This is a disease that causes diarrhea, bloody stool, and bleeding from organ tissues. It proliferates in intestinal cells, invades intestinal mucosa and submucosa and destroys these tissues), ammonia poisoning (damage to intestinal mucosa cells throughout the digestive tract due to increased ammonia in livestock) may occur. Due to these results, the increase in mortality rate and the slow growth of broilers eventually lead to a decrease in productivity, which further increases the damage to livestock farms. Accordingly, various approaches have been studied in order to solve the problem of increasing animal diseases caused by the regulation of the use of antibiotics.

For example, in Korean Patent Registration No. 877800, a study was conducted on improving feed efficiency, improving meat quality and increasing weight by including bamboo leaves or bamboo charcoal, and using leaves or extracts of red pine trees with antioxidant activity in the Republic of Korea In Patent Registration No. 804722, it was intended to contribute to the increase of the income of livestock farmers by shortening the breeding period, reducing feed cost, and increasing immunity. Similarly, Korean Patent Registration No. 777833 uses Schisandra extract, Korean Patent No. 881033 uses cockscomb extract, and Korean Patent Registration No. 406158 uses extracts of natural herbal medicines such as honeysuckle, honeysuckle, red ginseng, honeysuckle, Gyunmyung tea, licorice, and horseradish. It has been reported that the anti-inflammatory effect can be obtained even in animals by removing the toxicity. However, the prior literature reported so far is nothing more than adding a large amount of natural herbal medicines to the feed without any special processing, either by drying them and adding them to the feed as it is, or by adding a plurality of natural herbal medicines to the feed through a simple operation, promoting animal growth or In order to increase the weight gain, an approach for each individual component present in the extract has not been performed, and in particular, there is no disclosure of a means for achieving the desired purpose while keeping the amount of the animal feed additive blended in the feed extremely small.

Accordingly, the present inventors have researched a novel additive for animal feed that has not been reported before, which can promote the growth of animals, reduce the mortality rate of livestock, increase productivity, and replace the animal feed formulated with antibiotics. came to the invention.

To this end, in the present invention, there is provided a feed composition for promoting animal growth comprising a mugwort extract.

In addition, in the feed composition of the present invention, the mugwort extract is extracted using C1-6 alcohol including ethanol, 1-propanol, 2-propanol, 2-butanol, 1-butanol or 2-methyl-1propanol. There is provided a feed composition, characterized in that.

Furthermore, in the feed composition of the present invention, mugwort is yellow sea mugwort (Artemisia argyi), mugwort (A. princeps var. orientalis, Al asiatica), wild mugwort (A. montana), wormwood (A. mongolica) and Korean herbal medicines outside the Korean Pharmacopoeia. A feed composition is provided, characterized in that it is selected from plants and animals defined in the (crude drug) standard.

In addition, in the feed composition of the present invention, there is provided a feed composition characterized in that the mugwort is extracted by an immersion method, a reflux method, an ice-chilling method, or an ultrasonic method.

In addition, there is provided a method for producing the feed composition of claim 1, wherein the method for producing the feed composition of the present invention comprises mixing the soft algae extract obtained including the process of filtering and concentrating the mugwort extract and animal feed. .

As another feature of the present invention, there is provided a method for promoting animal growth, comprising administering a mugwort extract containing eupatylin and zeosidine obtained by extracting mugwort with lower alcohol.

In addition, in the animal growth promotion method, the lower alcohol is a C1-6 alcohol is provided, characterized in that the animal growth promotion method.

Furthermore, in the method for promoting animal growth, there is provided a method for promoting animal growth, characterized in that the dosage of the mugwort extract is 0.3 mg per kg of animal or less.

Hereinafter, a feed composition for promoting animal growth comprising mugwort extract, which is the first feature of the present invention, will be described.

Mugwort used in the present invention is yellow sea mugwort (Artemisia argyi), mugwort (A. princeps var. orientalis, A. asiatica), wild mugwort (A. montana), wormwood (A. mongolica), and Korean herbal medicine (crude medicine) standards outside the Korean Pharmacopoeia. Includes defined allografts. Mugwort is called ayeop in oriental medicine and is known to have anti-inflammatory, antipyretic, hemostasis, and diuretic effects. On the other hand, mugwort is also marketed as a drug intended for human administration, for example, there may be a styrene tablet containing a leaf extract. In addition to this, medicines for human administration using medicinal plants include Shinbaro capsule containing Chuna drug, Wiryongseon, Joins tablet containing Gwalugeun Hagocho, and Mesima EX acid containing Sanghwang mycelium body extract. However, it has been reported that when mugwort extract is administered to the human body, there is a gastric mucosal protective effect through gastrointestinal protection, but it is not known what effect appears when the mugwort extract is formulated in feed and administered to animals. It has not been reported on any correlation between the extract and animal growth promotion and mortality reduction. Accordingly, the present inventors came to the present invention by focusing on reducing the mortality rate and showing a high production index when a very small amount of mugwort extract was formulated in feed while researching a method of using various medicinal plants as an alternative to antibiotics. That is, in the present invention, the dosage of mugwort extract is set to be 0.3 mg per kg of body weight or less as a daily dose, which corresponds to 10% or less of 3 mg per kg set as an effective amount in the aebyeop extract used in styrene. . However, the ingredients confirmed to have pharmacological action in Article 12 (5) No. 2 of the Regulations on Approval, Reporting, and Review of Drugs (No. 2010-103, December 31, 2010) as notified by the Korea Food and Drug Administration should be used at the lowest daily dose. In the case of using less than 1/5, it is stipulated that it can be used as an additive for purposes other than the active ingredient. It indicates that it is difficult to expect. However, despite the fact that the amount of mugwort extract used in the present invention was 0.3 mg per kg or less, which is 10% or less of 3 mg per kg of the permitted amount of styrene tablets, included in the animal feed, effective animal weight gain effect, mortality rate reduction, and production index increase Such unexpected results were obtained, leading to the present invention.

In order to prepare the mugwort extract of the present invention, a lower alcohol may be used, and it is preferable to use a lower alcohol having C1 to C6 carbon atoms. Specific examples of the solvent may include ethanol, n-propanol, isopropanol, and alcohols having 4 carbon atoms, that is, 2-butanol, 1-butanol, and 2-methyl-1-propanol. In the present invention, it is possible to use a soft extract extracted from mugwort with lower alcohol, and in this case, 1 g of the soft extract can be adjusted to correspond to about 20 g of mugwort.

In the present invention, when mugwort is extracted with lower alcohol, eupatylin and zagiosidine are extracted at high concentrations. Specifically, when the mugwort extract is prepared by the extraction method of the present invention, the mugwort extract may contain eupatylin in an amount of 0.80 to 2.40% by weight and zagiosidine in an amount of 0.25 to 0.75% by weight. In the present invention, the mugwort extract containing eupatylin and azaosidine obtained by extracting mugwort with lower alcohol as described above can be blended with animal feed to promote animal growth and reduce mortality. Eupatylline and zeosidine are known to have a gastric mucosal protective effect when administered to the human body, but in the present invention, a novel use of promoting the growth of animals and reducing mortality by administering them to animals in very small amounts is disclosed. In addition to administering the mugwort extract, the technical idea of the present invention includes a method of promoting the growth of the animal by administering to the animal eupatylin and/or zeosidine extracted from mugwort using a lower alcohol.

As the extraction method used in the present invention, a conventional herbal extraction method, that is, an immersion method, a reflux method, a cold acupuncture method, or an ultrasonic method may be used.

To provide an additive for animal feed that can replace animal feed containing antibiotics by reducing the mortality rate of livestock and improving productivity.

Example 1-1> Preparation of mugwort extract

Add 1 kg of mugwort (A. princeps var. orientalis, A. asiatica) to 10 kg of 1-propanol (weight ratio 1:10) and then re-extract by adding 1-propanol to the residue. Thereafter, the extract was filtered and concentrated under reduced pressure at 65 degrees or less to prepare about 50 g of soft extract. 50 g of the prepared soft extract was quantified using liquid chromatography, and it was confirmed that upatilin was 1.4% by weight and zeosidine was 0.63% by weight.

<Example 1-2> Preparation of mugwort extract

1Kg of mugwort (A. princeps var. orientalis, A. asiatica) was added to 13Kg of 2-propanol (weight ratio 1:13), followed by re-extraction by adding 2-propanol to the residue. Thereafter, the extract was filtered and concentrated under reduced pressure at 65 degrees or less to prepare about 50 g of soft extract. 50 g of the prepared soft-shell extract was quantified using liquid chromatography, and it was confirmed that upatilin was 1.6 wt% and zeosidine was 0.68 wt%.

<Example 1-3> Preparation of mugwort extract

After extraction by adding 1 kg of Artemisia argyi to 10 kg of 1-propanol (weight ratio 1:10), 1-propanol is additionally added to the residue for re-extraction. Then, the extract was filtered and concentrated under reduced pressure at 65 degrees or less to prepare 50 g of soft extract. 50 g of the prepared soft-shell extract was quantified using liquid chromatography, and it was confirmed that upatilin was 1.3 wt% and zeosidine was 0.48 wt%.

<Example 1-4> Preparation of mugwort extract

1Kg of Artemisia argyi is added to 13Kg of 2-propanol (weight ratio 1:13), and after extraction, additional 2-propanol is added to the residue for re-extraction. Then, the extract was filtered and concentrated under reduced pressure at 65 degrees or less to prepare 50 g of soft extract. 50 g of the prepared soft algae extract was quantified using liquid chromatography, and it was confirmed that upatilin was 1.2 wt% and zeosidine was 0.5 wt%.

<Example 2> Preparation of mugwort extract mixture

After diluting 1 kg of the mugwort extract prepared in Example 1-2 with 4 kg wheat flour to 5 kg, a mixture of 5 kg wheat flour and mugwort extract was further added to 65 kg wheat flour and 30 kg calcium carbonate to make 100 kg. .

ingredient Starter (3wks) Finisher (2wks) yellow corn 47.29 59.13 Wheat 8.00 - Soybean meal 31.64 28.95 Corn gluten meal 3.88 3.74 Tallow 5.55 5.00 LysineHCl (78.5%) - 0.03 Dicalcium phosphate 1.90 1.40 DL-methionine (98%) 0.11 0.05 Limestone 1.03 1.12 Choline chloride (50%) 0.08 0.08 Salt 0.32 0.30 Vit. mixture 0.10 0.10 Min. mixture 0.10 0.10 Total 100.00 100.00 TMEn, kcal/kg 3,150 3,190 Crude protein, % 21.50 20.00 Ca, % 1.00 0.90 Available P, % 0.45 0.35 Lysine, % 1.10 1.05 Met + Cys, % 0.85 0.75

100 kg of the mugwort extract mixture prepared in this way and the general animal feed having the composition shown in Table 1 were mixed, and the final animal feed containing the mugwort extract was 200,000 kg, and fed to the animals. In the composition of Table 1, Vit. The mixture is a mixture of vitamin A 40,000 IU, vitamin D3 8,000 IU, vitamin E 10 IU, vitamin K3 4 mg, vitamin B1 4 mg, vitamin B2 12 mg, vitamin B6 6 mg, vitamin B12 20 μg, pantothenic acid 20 mg, folic acid 2 mg, nicotinic acid 60 mg. In addition, the mineral mixture contains Fe 30mg, Zn 25mg, Mn 20mg, Co 0.15mg, Cu 5mg, Se 0.1mg. A general animal feed containing no mugwort extract having the composition of Table 1 above was used as Comparative Example 1, and Example 2 and Comparative Example 1 were each fed for up to 5 weeks. <Experimental Example 1> Hepatotoxicity and weight gain test

Using the animal feed mixture of Example 2 and Comparative Example 1, a feeding experiment was carried out for a total of 21 days using 9,000 birds in Example 2 and 20,500 birds in Comparative Example 1 targeting 1-day-old broiler chicks, respectively. At this time, the daily dose of mugwort extract per kg was adjusted to an average of 0.25 mg (0.0020 mg/kg as eupatylin).

Comparative Example 1 Example 2 *P-value GOT 110.88 118.15 0.4680 GPT 10.55 9.50 0.6956

*P-value was calculated at 95% confidence interval.

Table 3 shows the results of analysis of liver function-related enzyme activity in broiler plasma fed with mugwort extract. The level of GOT in the blood can be used as an indicator to indicate the degree of damage to the liver and tissues in poultry, and can be an indicator to verify the safety when introducing a new alternative raw material or functional additive in the feed. Therefore, through the results in Table 3, it was confirmed that the supplementary feeding of mugwort extract did not have a negative effect on broilers.

Evaluation items Comparative Example 1 Example 2 *P-value Body weight (g) 929.25 991.13 0.057

Table 4 shows the results of measuring the weight gain of broilers fed with mugwort extract. It was confirmed that the weight gain of Example 2 was higher than that of Comparative Example 1. <Experimental Example 2> Productivity comparison

The number of 85,000 broiler chicks was compared to 38,000 in Comparative Example 1 and 53,000 in Example 2, respectively, and the productivity through feeding was investigated after a breeding experiment for a total of 33 days. At this time, the daily bathing amount of mugwort extract per kg was adjusted to an average of 0.26 mg (0.0021 mg/kg as eupatylin).

Item Comparative Example 1 Example 2 Mortality (%) 2.42 0.11 Average weight (kg) 1.460 1.507 production index 246 266

Table 5 shows the effect of mugwort extract on broiler productivity. In the case of Example 2 including the mugwort extract, the mortality rate was improved by about 95% or more compared to Comparative Example 1 without the mugwort extract, and the average body weight was also increased. In addition, in the case of Example 2 in the production index representing an objective relative indicator, it was seen that the result was increased by about 8% compared to Comparative Example 1. Based on the above results, only 0.26 mg of mugwort extract per kg could improve animal mortality, increase average weight, and maximize production index. It was confirmed that it can be used as a substitute and can contribute to the improvement of farm households' income.

Claims (11)

delete delete delete delete It is prepared by mixing the soft seaweed extract obtained including the process of filtering and concentrating the mugwort extract, and the mugwort extract mixture mixed with wheat flour and calcium carbonate, and animal feed,
The mugwort extract is extracted by adding 1-propanol or 2-propanol at least 10 times the weight of mugwort, followed by re-extraction by adding 1-propanol or 2-propanol to the residue; and
It is prepared by filtering the extract obtained by the re-extraction and concentrating under reduced pressure to prepare a mugwort extract in the form of soft algae extract,
The prepared mugwort extract contains 0.8 to 2.4% by weight of eupatylin and 0.25 to 0.75% by weight of zagiosidine,
The animal feed is corn (Yellow corn), wheat (Wheat), soybean meal (Soybean meal), corn gluten meal (Corn gluten meal), tallow (Tallow), lysine hydrochloride (LysineHCl), dibasic calcium phosphate (Dicalcium phosphate), DL-methionine (DL-methionine), limestone (Limestone), choline chloride (Choline chloride), salt (Salt), a vitamin mixture (Vit. mixture) and a feed composition comprising a mineral mixture (Min. mixture) manufacturing method.
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