KR102170850B1 - A composition for treating acne or improving acne skin - Google Patents

Abstract

The present invention relates to a composition for treating acne, comprising: extracts of ginger, green tea, purslane, cucumber, Centella asiatica, and broccoli; and trehalose. More specifically, the present invention relates to a composition for treating acne containing natural extracts to minimize skin irritation.

Description

Composition for treating acne or improving skin {A composition for treating acne or improving acne skin}

The present invention relates to a composition for treating acne or improving skin.

Acne (acne vulgaris) is a common skin disease in adolescents and adults, and is an inflammatory disease in the sebaceous glands of the skin.

As a result of analyzing data related to medical care benefits paid by the National Health Insurance Corporation from 2010 to 2015, the number of people who received acne treatment as the main diagnosis was counted as 110,758 in 2015, compared to 100,935 in 2010. % Increase. Among the total number of acne treatments, 20s were the most common at 43.1%, followed by teens at 31.1%.

The causes and types of acne appear differently by gender and age group. Hormonal changes in adolescence are the main cause of acne, and they appear mainly in areas called T zones such as forehead and nose.

On the other hand, acne in adults is often caused by complex reasons such as stress, drinking, and irregular lifestyle habits, and occurs in various parts of the body such as cheeks, chin, forehead, and neck. Adult acne occurs in pores clogged by sweat or waste products, and the recurrence rate is high.

If acne occurs and is left unattended, various problems can occur. Acne is not just an inflammation, but also causes problems in the dermal layer of the skin, causing various secondary damage such as acne marks, acne scars, and enlarged pores.

Acne is often the main cause of the habit, so it is recommended to manage it consistently in daily life. Therefore, there is a need to develop a composition for treating acne or a composition for improving acne skin, which minimizes unnecessary skin irritation during washing, protects the skin from external irritants, and exhibits a substantial antibacterial effect.

Korean Patent Application Publication No. 1999-000085

The present invention is to provide a composition for treating acne or a composition for improving acne skin containing a natural extract so as to minimize skin irritation while showing an antibacterial effect against acne causative bacteria in order to solve the above problems.

The present invention relates to a composition for treating acne or a composition for improving acne skin, including a ginger extract, a green tea leaf extract, a purslane extract, a cucumber extract, a centella asiatica extract and a broccoli extract.

The composition of the present invention may further comprise trehalose.

In the composition of the present invention, the ginger extract, green tea leaf extract, purslane extract, cucumber extract, centella extract and broccoli extract may contain 0.5 to 5% by weight, respectively, based on the total composition. Alternatively, in the composition of the present invention, the total amount of the ginger extract, green tea leaf extract, purslane extract, cucumber extract, centella extract and broccoli extract may be included in an amount of 3 to 30% by weight of the total composition.

The ginger extract, green tea leaf extract, purslane extract, cucumber extract, centella extract and broccoli extract may be extracted from water, an organic solvent, or a mixture thereof. For example, ginger extract, green tea leaf extract, purslane extract, cucumber extract, centella extract, and broccoli extract are the group consisting of ginger, green tea leaf, purslane, cucumber, centella and broccoli, respectively, water, methanol, ethanol, ethyl acetate, and hexane It can be extracted by a method such as a cold precipitation method, a warm precipitation method, or a constant temperature vacuum extraction method using one or a mixture of two or more selected from as an extraction solvent. Preferably, water, ethanol, or a mixture of water and ethanol is used as the extraction solvent.

At this time, each extract is independently extracted from each natural product (i.e., ginger, green tea leaves, purslane, cucumber, centella asiatica and broccoli, respectively), or after mixing all of ginger, green tea leaves, purslane, cucumber, centella asiatica, and broccoli It can be extracted simultaneously from this mixture.

The composition of the present invention may be formulated as a liquid formulation, suspension or emulsion composition. It is preferably formulated as an emulsion composition.

The composition of the present invention may be used as an acne treatment drug or cosmetic or cleaning agent.

The composition of the present invention comprises the steps (a) of separately adding ginger, green tea leaves, purslane, cucumber, centella asiatica and broccoli into an extraction solvent, respectively, and extracting at warm, room temperature, or cold temperature; (B) drying the extract obtained from step (a) to obtain a solid extract; And mixing the solid extracts of ginger, green tea leaves, purslane, cucumber, centella asiatica and broccoli.

Here, as the extraction solvent, one or a mixture of two or more selected from the group consisting of water, methanol, ethanol, ethyl acetate, and hexane may be used.

Here, the step (c) includes dissolving the extracts of ginger, green tea leaves, purslane, cucumber, centella and broccoli in an organic solvent; And emulsifying an organic solvent in which extracts of ginger, green tea leaves, purslane, cucumber, centella and broccoli are dissolved in water together with a surfactant (for example, trehalose) to prepare an emulsion composition. .

In addition, the composition of the present invention includes the steps (a) of putting ginger, green tea leaves, purslane, cucumber, centella asiatica and broccoli all in an extraction solvent, and extracting at warm, room temperature or cold temperature; (B) drying the extract obtained from step (a) to obtain a solid extract; And it may be prepared including the step (c) of preparing a composition comprising the solid extract.

Here, the extraction solvent may be one or a mixture of two or more selected from the group consisting of water, methanol, ethanol, ethyl acetate, and hexane.

In addition, the step (c) here, the step of dissolving the solid extract of the ginger, green tea leaves, purslane, cucumber, centella and broccoli in an organic solvent; And emulsifying an organic solvent in which solid extracts of ginger, green tea leaves, purslane, cucumber, centella and broccoli are dissolved in water together with a surfactant (for example, trehalose, etc.) to prepare an emulsion composition. have.

Ginger (Zingiber officinale) is a perennial plant of the ginger family Ginger family, a monocotyledonous plant, and its roots are used for edible and medicinal purposes, and are known to be effective in promoting gastric juice secretion and enhancing digestive power.

Green tea leaves are the leaves of the tea tree (Camellia sinensis) and are known to have antioxidant effects.

Purslane (Portulaca oleracea) is a perennial plant of the family Purslane family, a dicotyledonous plant, and dried outpost is used for medicinal purposes, and is known to be effective in preventing arteriosclerosis.

Cucumi (Cucumis sativus) is a perennial vine plant in the family Plywood group of dicotyledons. The fruit is used for food and is known to be effective in discharging waste products from the body.

Centella asiatica is a perennial plant of the Aperaceae family of umbela in the dicotyledonous plant Ipanhwa group. Dried outposts are used for medicinal purposes and are known to have the effect of protecting the gastric mucosa.

Broccoli (Brassica oleracea var. italica) is an annual plant of the mustard family of the dicotyledonous plant Poppy.Italica is known to help bone health by promoting the absorption of calcium by using a small flower cluster attached to the end of the stem for food or medicinal purposes.

The composition according to the present invention contains a natural extract, and is safe because it can minimize skin irritation while showing an antibacterial effect against acne-causing bacteria.

1 is a photograph of the results of testing the antimicrobial activity of the composition according to the present invention by the disk diffusion method.
2 is a photograph and a graph taking the results of testing the antibacterial activity of the composition according to the present invention by optical analysis.

Hereinafter, the present invention will be described in detail by examples. However, the following examples are merely illustrative of the present invention, and the spirit and scope of the present invention are not limited by the following examples.

Example 1: natural product extraction

After washing 100 g of ginger with water, it is dried. The dried product is pulverized with a pulverizer (Pulverizer 2500A pulverizer manufactured by Yunbang, China). This pulverized product was put into 3 L of 70% ethanol, and leached for 5 days while stirring at room temperature. After centrifuging the leachate at 1000 rpm, the supernatant was collected and filtered, and the filtrate was dried to obtain 10 g of a solid extract.

For green tea leaves, purslane, cucumber, centella and broccoli, 9g, 10g, 11g, 9g and 9g of extracts were obtained through the same process as above.

Example 2: Identification of indicator substances

Each extract obtained in Example 1 was purified with hexane and ethyl acetate. For each of the purified extracts, an indicator substance was separated and confirmed using HPLC.

HPLC analysis conditions are as follows:

Column: SUPELCOSIL ^TM LC-NH2 HPLC column (Sigma-Aldrich) with a pore diameter of 5 μm in a stainless steel pipe with an inner diameter of about 3 mm and a length of 25 cm.

Mobile phase: methanol-3 distilled water (46:35, v/v) (degassifying)

Flow rate: 1.1 mL/min

Column temperature: 22 ℃

Injection volume: 50 µl

Detector and wavelength: UV absorbance photometer 310 ㎚ (Bio-Rad)

Each indicator substance is as follows:

Natural products Indicator material constitutional formula Chemical name ginger

6-gingerol Green tea leaves

(-)-epigallocatechin-3-gallate cucumber

β-sitosterol purslane

Daucosterol Centella

Asiatic acid broccoli

Glucoraphanin

Example 3: Preparation of emulsion composition

A composition of 100 ml volume is prepared as follows in the composition and content of Table 2 below:

First, each component of the oil phase is mixed to prepare an oil phase mixture. The active part (that is, each solid extract obtained in Example 1) was added thereto, heated to 80° C., and dissolved while stirring at low speed by applying a pressure of 220 bar.

Separately, the oil phase in which the solid extracts are dissolved is added to the aqueous phase mixture in which each component of the aqueous phase is mixed together with a surfactant, and then marked to be 100 ml with purified water while maintaining the temperature at 75°C. While maintaining this mixture at a temperature of 75° C., the mixture was stirred for 4 hours in a low-rotating stirrer to prepare an emulsion composition according to the present invention.

division ingredient Content (g/100ml) Active part Ginger solid extract 6 Green tea leaf solid extract 3 Purslane solid extract 6 Cucumber solid extract 4 Centella asiatica solid extract 4 Broccoli solid extract 5 Award Purified water Appropriate amount Triethanolamine 0.1 glycerin 3 Sorbitol One Propylene glycol 2 BHT 0.1 Allantoin 2 Hydrolyzed Collagen 1.5 Citric acid One Magnesium ascorbyl phosphate One Sodium hyaluronate 4 Paid part Myristic acid 18 Stearic acid 12 Beta-carotene 2.5 Tetrasodium EDTA 0.4 Spices 0.04 Surfactants Trehalose 9

Example 4: Antibacterial test against C. acnes

With respect to the emulsion composition of Example 3, an antibacterial test of C. acnes (Cutibacterium acnes), which is acne bacteria, was performed by a disk diffusion method as follows.

As a control, a composition containing a solid extract of ginger, a solid extract of green tea leaf, a solid extract of purslane, a solid extract of cucumber, a solid extract of centella asiatica, and a solid extract of broccoli (each prepared in Example 1) and an antibiotic (ampicillin) alone ( Comparative Examples 1 to 7) were used.

The composition of each control group (Comparative Examples 1 to 7) is shown in Table 3 below (unit of content (g/100ml)):

Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Ginger solid extract 6 - - - - - - Green tea leaf solid extract - 3 - - - - - Purslane solid extract - - 6 - - - - Cucumber solid extract - - - 4 - - - Centella asiatica solid extract - - - - 4 - - Broccoli solid extract - - - - - 5 - Ampicillin - - - - - - One Purified water Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Triethanolamine 0.1 0.1 0.1 0.1 0.1 0.1 - glycerin 3 3 3 3 3 3 - Sorbitol One One One One One One - Propylene glycol 2 2 2 2 2 2 - BHT 0.1 0.1 0.1 0.1 0.1 0.1 - Allantoin 2 2 2 2 2 2 - Hydrolyzed
Collagen 1.5 1.5 1.5 1.5 1.5 1.5 - Citric acid One One One One One One - Magnesium Ascorbyl
Phosphate One One One One One One - Sodium hyaluronate 4 4 4 4 4 4 - Myristic acid 18 18 18 18 18 18 - Stearic acid 12 12 12 12 12 12 - Beta-carotene 2.5 2.5 2.5 2.5 2.5 2.5 - Tetrasodium EDTA 0.4 0.4 0.4 0.4 0.4 0.4 - Spices 0.04 0.04 0.04 0.04 0.04 0.04 - Trehalose 9 9 9 9 9 9 -

In addition, a composition using sucrose instead of trehalose as a surfactant in the composition of Example 3 was used as a control (Comparative Example 8) (the content of each component was the same).

The composition of Comparative Example 8 is shown in Table 4 below:

division ingredient Content (g/100ml) Active part Ginger solid extract 6 Green tea leaf solid extract 3 Purslane solid extract 6 Cucumber solid extract 4 Centella asiatica solid extract 4 Broccoli solid extract 5 Award Purified water Appropriate amount Triethanolamine 0.1 glycerin 3 Sorbitol One Propylene glycol 2 BHT 0.1 Allantoin 2 Hydrolyzed Collagen 1.5 Citric acid One Magnesium ascorbyl phosphate One Sodium hyaluronate 4 Paid part Myristic acid 18 Stearic acid 12 Beta-carotene 2.5 Tetrasodium EDTA 0.4 Spices 0.04 Surfactants Sucrose 9

After inoculating the C. acnes strain in a plate medium mixed with Reinforced Clostridial Medium (RCM) and Bacteriological agar, 30 μl of any one of the compositions of Example 3 and Comparative Examples 1 to 8 was inoculated into each disc (diameter 8 mm). After loading, the plate was tightly wrapped with parafilm to block oxygen.

After culturing the RCM medium in a 37° C. incubator for 18 hours, the antimicrobial activity inhibition radius for the composition of Example 3 and the compositions of Comparative Examples 1 to 7 was measured. The experimental results are shown in Table 5 and FIG. 1 (here, the suppression radius is a value obtained by subtracting the disk area from the area of the circle, and the unit is mm ² ).

Test substance Suppression radius (unit mm ² ) Composition of Example 3 5 Antibiotic (Ampicillin) (Comparative Example 7) 7 Ginger extract (Comparative Example 1) 0.1 Green Tea Leaf Extract (Comparative Example 2) 0.1 Purslane extract (Comparative Example 3) 0.1 Cucumber Extract (Comparative Example 4) 0.1 Centella asiatica extract (Comparative Example 5) 0.1 Broccoli Extract (Comparative Example 6) 0.1 Use of sucrose (Comparative Example 8) 0.1

end. Comparison of antibacterial activity of each natural product extract

From the experimental results, when each extract was used alone (compositions of Comparative Examples 1 to 6), antibacterial activity against C. acnes was hardly observed, but it was confirmed that the composition of Example 5 according to the present invention showed antibacterial activity. Could

The composition of Example 3 according to the present invention slightly did not reach the antibacterial effect of the positive control, ampicillin antibiotic, but in view of the fact that side effects such as resistance development occur when antibiotics are administered, the composition of the present invention is a safe herbal extract It was found that it showed satisfactory antimicrobial activity with few side effects.

I. Difference in antibacterial activity according to surfactant component

When sucrose was used as the surfactant (Comparative Example 8), the antimicrobial activity against C. acnes was less than that of the composition of Example 3 using trehalose.

Example 5: Optical analysis for antibacterial activity

10 ml of RCM medium (dispersed in distilled water) in which the C. ances strain is present at a concentration of 10 ⁸ /ml is added to a test tube. Here, the composition of Example 3 and the composition of Comparative Example 8 were added and mixed so as to have a concentration of 10 μg/ml or 30 μg/ml with respect to the final mixture, respectively. The test tube containing this mixture was incubated for 18 hours in a shaking incubator at 37°C.

Then, the absorbance (OD 600nm) was optically analyzed to analyze the concentration of the strain. The analysis results are shown in FIG. 2.

As a result of the analysis, antimicrobial activity was observed for the composition of the present invention using trehalose, but the antimicrobial activity was hardly observed in the composition of the control group using sucrose.

This is presumed to be due to the fact that the antimicrobial activity was lowered due to poor emulsification when sucrose was used.

As described above, it was confirmed that trehalose was suitable as a surfactant in the composition of the present invention.

The composition of the present invention is a safe composition capable of minimizing skin irritation while exhibiting an antibacterial effect against acne causative bacteria.

Claims (14)

A pharmaceutical composition for the treatment of acne comprising each extract of ginger, green tea, purslane, cucumber, centella, broccoli and trehalose. delete The composition of claim 1, wherein the formulation is an emulsion. A composition for improving acne skin containing extracts of ginger, green tea, purslane, cucumber, centella asiatica, broccoli, and trehalose. The composition according to claim 4, which is used as a cosmetic. The composition according to claim 4, which is used for cleaning. delete The composition of claim 4, wherein the formulation is an emulsion. (A) adding ginger, green tea leaves, purslane, cucumber, centella asiatica, and broccoli into an extraction solvent, respectively, and extracting at warm, room temperature, or cold temperature;
(B) drying the extract obtained from step (a) to obtain a solid extract;
Including the step (c) mixing each solid extract of ginger, green tea leaves, purslane, cucumber, centella and broccoli,
Here, the step (c),
Dissolving the ginger, green tea leaves, purslane, cucumber, centella asiatica and broccoli extracts in an organic solvent; And
The composition or agent for treating acne of claim 1 comprising the step of preparing an emulsion composition by emulsifying an organic solvent in which the extracts of ginger, green tea leaves, purslane, cucumber, centella and broccoli are dissolved in water together with trehalose A method for preparing a composition for improving acne skin of claim 4. delete delete (A) adding ginger, green tea leaves, purslane, cucumber, centella asiatica and broccoli to the extraction solvent, and extracting at warm, room temperature or cold temperature;
(B) drying the extract obtained from step (a) to obtain a solid extract;
Including the step (c) of preparing a composition comprising the solid extract,
Here, the step (c),
Dissolving the solid extracts of the ginger, green tea leaves, purslane, cucumber, centella asiatica and broccoli in an organic solvent; And
The composition for treating acne of claim 1 comprising the step of preparing an emulsion composition by emulsifying an organic solvent in which solid extracts of ginger, green tea leaves, purslane, cucumber, centella and broccoli are dissolved in water together with trehalose and emulsified. A method for producing a composition for improving acne skin of claim 4. delete delete

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