WO2021261971A1 - Composition for acne treatment or skin improvement - Google Patents

Abstract

A composition for acne treatment is disclosed.

Description

Composition for treating acne or improving skin

The present invention relates to a composition for treating acne or improving skin.

Acne vulgaris (acne vulgaris) is a common skin disease in adolescents and adults, and is an inflammatory disease that appears in the sebaceous glands of the skin.

As a result of analyzing the data related to the medical care benefits paid by the National Health Insurance Corporation from 2010 to 2015, the number of people who received acne treatment as the main diagnosis was 110,758 in 2015, which is about 10 compared to 100,935 in 2010. % will increase. Among the total number of people who received acne treatment, those in their 20s accounted for the most at 43.1%, followed by those in their teens with 31.1%.

The causes and types of acne vary by gender and age group. In adolescence, hormonal changes are the main cause of acne, and it mainly appears in areas called T-zone, such as the forehead and nose.

On the other hand, adult acne is mainly caused by complex reasons such as stress, drinking, and irregular lifestyle, and occurs in various parts of the body such as the cheeks, chin, forehead, and neck. Adult acne occurs in pores clogged with sweat or waste products, and the recurrence rate is high.

If acne is left unattended after it occurs, various problems can occur. Acne is not just a simple inflammation, but also causes problems in the dermis layer of the skin, causing various secondary damages such as acne marks, acne scars, and enlarged pores.

Since acne is often the main cause of lifestyle, it is recommended to manage it consistently in daily life. Therefore, there is a need to develop a composition for treating acne or a composition for improving acne prone skin that minimizes unnecessary skin irritation during washing, protects the skin from external irritants, and exhibits a substantial antibacterial effect.

In order to solve the above problems, the present invention is to provide a composition for treating acne or a composition for improving acne prone skin containing a natural extract to minimize skin irritation while exhibiting an antibacterial effect on acne-causing bacteria.

The present invention relates to a composition for treating acne or a composition for improving acne prone skin, comprising a ginger extract, green tea leaf extract, purslane extract, cucumber extract, centella asiatica extract and broccoli extract.

The composition of the present invention may further comprise trehalose.

In the composition of the present invention, the ginger extract, green tea leaf extract, purslane extract, cucumber extract, centella asiatica extract and broccoli extract may each contain 0.5 to 5% by weight based on the total composition. Alternatively, the total of the ginger extract, green tea leaf extract, purslane extract, cucumber extract, Centella asiatica extract and broccoli extract in the composition of the present invention may be included in an amount of 3 to 30% by weight of the total composition.

The ginger extract, green tea leaf extract, purslane extract, cucumber extract, centella asiatica extract and broccoli extract may be extracted from water, an organic solvent, or a mixture thereof. For example, ginger extract, green tea leaf extract, purslane extract, cucumber extract, centella asiatica extract and broccoli extract are each of ginger, green tea leaf, purslane, cucumber, centella asiatica and broccoli in water, methanol, ethanol, ethyl acetate and hexane. One or a mixture of two or more selected from the group consisting of an extraction solvent may be used for extraction by a method such as cold acupuncture, warm acupuncture, or constant temperature vacuum extraction. Preferably, water, ethanol, or a mixture of water and ethanol is used as the extraction solvent.

At this time, each extract is independently extracted from each natural product (that is, each of ginger, green tea leaf, purslane, cucumber, centella asiatica and broccoli), or after mixing all of ginger, green tea leaf, purslane, cucumber, centella asiatica and broccoli It can be extracted simultaneously from this mixture.

The composition of the present invention may be formulated as a liquid formulation, suspension or emulsion composition, and the like. Preferably formulated as an emulsion composition.

The composition of the present invention may be used as a pharmaceutical or cosmetic for treating acne or for cleaning.

The composition of the present invention, ginger, green tea leaves, purslane, cucumber, centella asiatica and broccoli separately in an extraction solvent, respectively, and extracting at warm, room temperature or cold temperature (a); drying the extract obtained from step (a) to obtain a solid extract (b); And it can be prepared including the step (c) of mixing each solid extract of ginger, green tea leaf, purslane, cucumber, centella asiatica and broccoli.

Here, as the extraction solvent, one or a mixture of two or more selected from the group consisting of water, methanol, ethanol, ethyl acetate and hexane may be used.

Here, the step (c) comprises dissolving each extract of ginger, green tea leaf, purslane, cucumber, centella asiatica and broccoli in an organic solvent; and emulsifying an organic solvent in which each extract of ginger, green tea leaf, purslane, cucumber, centella asiatica and broccoli is dissolved in water together with a surfactant (eg, trehalose, etc.) to prepare an emulsion composition. .

In addition, the composition of the present invention, ginger, green tea leaves, purslane, cucumber, centella asiatica and broccoli all put in an extraction solvent, and extracting at warm, room temperature or cold temperature (a); drying the extract obtained from step (a) to obtain a solid extract (b); And it may be prepared including the step (c) of preparing a composition comprising the solid extract.

Here, the extraction solvent may be one or a mixture of two or more selected from the group consisting of water, methanol, ethanol, ethyl acetate and hexane.

In addition, the step (c) here, the step of dissolving the solid extract of ginger, green tea leaf, purslane, cucumber, centella asiatica and broccoli in an organic solvent; and preparing an emulsion composition by emulsifying an organic solvent in which the solid extract of ginger, green tea leaves, purslane, cucumber, centella asiatica and broccoli is dissolved in water together with a surfactant (eg, trehalose, etc.) have.

Ginger (Zingiber officinale) is a monocotyledonous perennial plant of the Ginger family, and its root is used for food and medicine, and is known to be effective in promoting gastric juice secretion and enhancing digestion.

Green tea leaves are leaves of the tea tree (Camellia sinensis) and are known to have antioxidant effects.

Purslane (Portulaca oleracea) is an annual plant of the dicotyledonous purslane family, and dried out herbs are used medicinally and are known to be effective in preventing arteriosclerosis.

Cucumber (Cucumis sativus) is an annual vine of the dicotyledonous plywood family, Gourdaceae, and its fruits are used for food and are known to have the effect of excreting wastes from the body.

Centella asiatica is a dicotyledonous perennial plant of the Dicotyledonous family Umbilicalaceae family, and dried out plants are used medicinally and are known to have the effect of protecting the gastric mucosa.

Broccoli (Brassica oleracea var. italica) is an annual plant of the dicotyledonous papaveraceae mustard family, and small flower buds hanging from the tip of the stem are used for food or medicine, and it is known to promote calcium absorption and help bone health.

The composition according to the present invention contains a natural extract, and is safe because it can minimize skin irritation while exhibiting an antibacterial effect on acne-causing bacteria.

1 is a photograph of the results of testing the antimicrobial activity of a composition according to the present invention by a disk diffusion method.

2 is a photograph and graph of the results of testing the antimicrobial activity of the composition according to the present invention by optical analysis.

Hereinafter, the present invention will be described in detail by way of Examples. However, the following examples are merely illustrative of the present invention, and the spirit and scope of the present invention are not limited by the following examples.

Example 1: Natural product extraction

100 g of ginger is washed with water and dried. The dried ones are pulverized with a pulverizer (Pulverizer 2500A pulverizer manufactured by Yunbang, China). This pulverized product is placed in 3 liters of 70% ethanol and leached for 5 days while stirring at room temperature. After centrifuging the leachate at 1000 rpm, the supernatant was collected and filtered, and the filtrate was dried to obtain 10 g of a solid extract.

9g, 10g, 11g, 9g and 9g of extracts from green tea leaf, purslane, cucumber, centella asiatica and broccoli were obtained through the same process, respectively.

Example 2: Identification of indicator substances

Each extract obtained in Example 1 was purified with hexane and ethyl acetate. For each of the purified extracts, an indicator material was isolated and confirmed using HPLC.

HPLC analysis conditions are as follows:

^{Column: SUPELCOSIL TM} LC-NH2 HPLC column (Sigma-Aldrich) with a pore diameter of 5 μm in a stainless steel tube with an inner diameter of about 3 mm and a length of 25 cm (Sigma-Aldrich)

Mobile phase: methanol-3 distilled water (46:35, v/v) (degassifying)

Flow rate: 1.1 mL/min

Column temperature: 22 ℃

Injection volume: 50 μl

Detector and wavelength: UV absorbance meter 310 nm (Bio-Rad)

Each indicator substance is as follows:

natural product	indicator substance
	constitutional formula	chemical name
ginger		6-gingerol
green tea leaves		(-)-epigallocatechin-3-gallate
cucumber		β-sitosterol
purslane		Daucosterol
Centella asiatica		Asiatic acid
broccoli		Glucoraphanin

Example 3: Preparation of emulsion composition

A composition with a capacity of 100 ml was prepared as follows with the composition and content of Table 2 below:

First, each component of the oil phase is mixed to prepare an oil phase mixture. The active part (that is, each solid extract obtained in Example 1) was put there, heated to 80° C., and dissolved while stirring at low speed by applying a pressure of 220 bar.

Separately, the oil phase in which each solid extract is dissolved is added to the aqueous phase mixture in which each component of the aqueous phase is mixed together with a surfactant, and then marked with purified water to make 100 ml while maintaining the temperature at 75°C. While maintaining the mixture at a temperature of 75° C., the mixture was stirred on a low-speed stirrer for 4 hours to prepare an emulsion composition according to the present invention.

Example 4: Antibacterial test against C. acnes

With respect to the emulsion composition of Example 3, an antibacterial test for C. acnes (Cutibacterium acnes), which is an acne bacterium, was performed by the disk diffusion method as follows.

As a control, a composition containing a ginger solid extract, a green tea leaf solid extract, a purslane solid extract, a cucumber solid extract, a centella asiatica solid extract, and a broccoli solid extract (each prepared in Example 1 was used) and an antibiotic (ampicillin) alone ( Comparative Examples 1 to 7) were used.

The composition of each control group (Comparative Examples 1 to 7) is shown in Table 3 below (unit of content (g/100ml)):

In addition, in the composition of Example 3, a composition using sucrose instead of trehalose as a surfactant was used as a control (Comparative Example 8) (the content of each component is the same).

The composition of Comparative Example 8 is shown in Table 4:

After inoculating the C. acnes strain on a plate medium mixed with Reinforced Clostridial Medium (RCM) and Bacteriological agar, 30 μl of any one of the compositions of Examples 3 and Comparative Examples 1 to 8 therein for each disk (diameter 8 mm) After loading, the plate was tightly wrapped with parafilm to block oxygen.

After culturing the RCM medium in an incubator at 37° C. for 18 hours, the antibacterial activity inhibition radius for the composition of Example 3 and the compositions of Comparative Examples 1 to 7 was measured. The experimental results are shown in Table 5 and FIG. 1 (here, the suppression radius is a value obtained by subtracting the disk area from the area of the circle, and the unit is mm ² ).

go. Comparison of antibacterial activity of each natural extract

From the experimental results, when each extract was used alone (composition of Comparative Examples 1 to 6), antibacterial activity against C. acnes was hardly observed, but it was confirmed that the composition of Example 5 according to the present invention exhibited antibacterial activity. could

Although the composition of Example 3 according to the present invention did not achieve the antibacterial effect of the positive control, ampicillin antibiotic, side effects such as resistance expression occurred during administration of the antibiotic, the composition of the present invention was a safe herbal extract. It was found that it exhibits satisfactory antibacterial activity with few side effects.

me. Differences in antibacterial activity according to surfactant components

When sucrose was used as a surfactant (Comparative Example 8), the antibacterial activity against C. acnes was less than that of the composition of Example 3 using trehalose.

Example 5: Optical analysis for antibacterial activity

10 ml of RCM medium (dispersed in distilled water) in which the C. ances strain is ^{present at a concentration of 10 8 /ml is placed in a test tube.} Here, the composition of Example 3 and the composition of Comparative Example 8 are put and mixed to have a concentration of 10 μg/ml or 30 μg/ml with respect to the final mixture, respectively. The test tube containing this mixture was incubated for 18 hours in a shaking incubator at 37°C.

Then, by optically analyzing the absorbance (OD 600nm), the concentration of the strain was analyzed. The analysis results are shown in FIG. 2 .

As a result of the analysis, antibacterial activity was shown for the composition of the present invention using trehalose, but almost no antibacterial activity was shown in the composition of the control group using sucrose.

It is presumed that this is because, when sucrose is used, emulsification is not performed properly and the antibacterial activity is lowered.

As such, it was confirmed that trehalose was suitable as a surfactant in the composition of the present invention.

The composition of the present invention is a safe composition that can minimize skin irritation while exhibiting an antibacterial effect on acne-causing bacteria.

Claims (14)

1. A pharmaceutical composition for treating acne comprising extracts of ginger, green tea, purslane, cucumber, centella asiatica, and broccoli.
2. The composition of claim 1 , further comprising trehalose.
3. The composition of claim 1 , wherein the formulation is an emulsion.
4. A composition for improving acne skin comprising extracts of ginger, green tea, purslane, cucumber, centella asiatica, and broccoli.
5. The composition according to claim 4, wherein the composition is used as a cosmetic.
6. The composition according to claim 4, characterized in that it is used for cleaning.
7. 5. The composition of claim 4, further comprising trehalose.
8. 5. The composition of claim 4, wherein the formulation is an emulsion.
9. Ginger, green tea leaf, purslane, cucumber, centella asiatica and broccoli are separately put in an extraction solvent, respectively, and extracted at warm, room temperature or cold temperature (a);

   Drying the extract obtained from step (a) to obtain a solid extract (b);

   A method of producing a composition for treating acne or improving acne skin, comprising the step (c) of mixing each solid extract of ginger, green tea leaves, purslane, cucumber, centella asiatica and broccoli.
10. The method according to claim 9, wherein the extraction solvent is one or a mixture of two or more selected from the group consisting of water, methanol, ethanol, ethyl acetate and hexane.
11. The method of claim 9, wherein step (c) comprises:

    Dissolving each extract of ginger, green tea leaf, purslane, cucumber, centella asiatica and broccoli in an organic solvent;

    A manufacturing method comprising the step of preparing an emulsion composition by emulsifying an organic solvent in which each extract of ginger, green tea leaf, purslane, cucumber, centella asiatica and broccoli is dissolved in water together with a surfactant.
12. Ginger, green tea leaves, purslane, cucumber, centella asiatica and broccoli all put in an extraction solvent, and extracting at warm, room temperature or cold temperature (a);

    drying the extract obtained from step (a) to obtain a solid extract (b);

    A method of preparing a composition for treating acne or improving acne skin, comprising the step (c) of preparing a composition comprising the solid extract.
13. The method according to claim 12, wherein the extraction solvent is one or a mixture of two or more selected from the group consisting of water, methanol, ethanol, ethyl acetate and hexane.
14. The method of claim 12, wherein step (c) comprises:

    dissolving the solid extracts of ginger, green tea leaves, purslane, cucumber, centella asiatica and broccoli in an organic solvent;

    A manufacturing method comprising the step of preparing an emulsion composition by emulsifying an organic solvent in which the ginger, green tea leaves, purslane, cucumber, centella asiatica and broccoli solid extracts are dissolved in water together with a surfactant.

Images