KR102155897B1 - Methods for extracting compound byproduct of Rubus coreans miquel and their composition containing compound byproducts - Google Patents

Abstract

The present invention relates to a method for stirring and extracting a byproduct extract of Rubus coreanus Miquel for scalp care and prevention or mitigation of dandruff and hair loss, and a composition containing the same. More specifically, the present invention relates to: a method for extracting a byproduct extract of Rubus coreanus Miquel, which uses a solvent of water/ethanol = 50/50 wt% and comprises a drying step, a grinding step, an ethanol addition step, an extraction step, a filtration step, a concentration step, a freeze-drying step, and a pulverization step; and to a composition containing the byproduct extract of Rubus coreanus Miquel. According to the present invention, a raw material stirring method comprises a step of preparing raw materials and a step of acquiring a composition containing the byproduct extract of Rubus coreanus Miquel from the stirred raw materials.

Description

Method for stirring and extracting bokbunja by-product extract, and composition containing the bokbunja byproduct extract {Methods for extracting compound by product of Rubus coreans miquel and their composition containing compound byproducts}

The present invention relates to a method for stirring and extracting the bokbunja by-product extract, and a composition containing the bokbunja by-product extract, and more particularly, drying, grinding, and ethanol addition using a solvent of water/ethanol=50/50,wt% It relates to a method for extracting a bokbunja by-product extract comprising the steps of fruit, extraction, filtration, concentration, lyophilization and crushing, and a composition containing the bokbunja by-product extract.

The present invention also improves the working environment in performing the process of stirring and processing various raw materials (e.g., related materials for manufacturing bokbunja, cheonnyeoncho, etc.) more effectively and stably, and performing the process of inputting and processing such raw materials in large quantities or repeatedly. And, it relates to a method of stirring raw materials that allows effective maintenance and maintenance by imparting a seismic effect to the stirring means itself.

The method of stirring the bokbunja by-product extract may be a raw material Gyobang method.

Research and development of various ingredients are being conducted to improve health-related issues. One of them is bokbunja. Modern people are always exposed to oxidative stress caused by oxygen, and this oxidative stress is normally removed by the in vivo antioxidant system. However, since industrialization, various environmental pollutants, smoking, alcohol, and radiation are increasing stress on the human body, and if these stresses are not removed, arteries due to damage to biological membranes, modification and loss of function of polymer proteins and DNA, etc. Since various diseases such as sclerosis, cancer, neurodegenerative diseases, and aging can be caused, interest in antioxidants for solving stress is focused.

Rubus coreanum Miquel is a deciduous shrub belonging to the Rosaceae family. It grows wild in the sunny areas of relatively high mountains in China, Japan, the southern regions of Korea, and Jeju Island and the central region. Fruits mature in June-July and turn dark red. Changes. The fruit of this bokbunja strawberry is called bokbunja (Rubi Fructus), and it is used for food like alcohol, Korean confectionery, and other beverages, and it has been collected when the fruit is immature and slightly boiled in boiling water and then used as a medicinal herb. .

In herbal medicine, bokbunja is known to have the effect of protecting the sinjeong (腎精), birth (胎生), eumwi (陰.), the sin (身), and the liver (肝) and brightening the eyes. Bokbunja contains ingredients such as total sugar (173%), reducing sugar (86%), crude protein (106%), crude meal (45%), crude fat (31%) and crude fiber (39%), and contains amyl alcohol. 11 types of alcohols, acids including valeric acid, 20 types of carbonyls including hexanal, 5 types of hydrocarbons including 2-heptanone, and methyl palmitate. It contains three kinds of esters of fragrance components. Recently, due to changes in diet and environment, and an increase in various stresses, the number of adults suffering from dandruff, hair loss or thin hair is increasing. Dandruff and alopecia are diseases that are lost in the area where hair should normally exist, and mainly occur on the head, and the cause is not yet clearly identified, but the genetic predisposition, endocrine abnormalities, mental causes, autoimmune reactions, other infectious diseases, It is estimated that trauma, atopy and seborrheic dermatitis are involved. Human hair becomes contaminated with contaminants upon contact with the surrounding atmosphere and is contaminated by sebum secreted from the scalp itself. Shampoo removes contaminants or sebum. Shampoos or conditioners, which are active ingredients composed of chemical components such as surfactants or silicones, cause seborrheic dermatitis or inflammatory diseases in people with sensitive skin, which not only causes hair loss, but also causes dandruff and itchiness due to dead skin cells. There is a problem indicating the side effects of. Therefore, efforts have been made to solve this problem by using natural materials. In recent years, research and development of shampoos having a protective function and nutrition supply function for the scalp and hair are being actively conducted. In particular, various methods of preparing shampoos have been proposed by adding plant extracts obtained from nature to shampoo raw materials (a raw material in which additives such as surfactants and foam enhancers are mixed). However, the shampoo or conditioner composition including most of the natural ingredients is in most cases selectively containing only one or two natural ingredients depending on the purpose.

On the other hand, there is also a problem in the absence of a stirring device capable of operating and performing more stable and comfortable processing in the technology for stirring the above-described components.

Republic of Korea Patent Publication No. 10-2009-0061218

In the present invention, various raw materials are stirred and processed more effectively and stably, and in performing the process of inputting and processing such raw materials in large quantities or repeatedly, the present invention improves the working environment and imparts a seismic effect to the stirring means itself to effectively manage maintenance. It is an object of the present invention to provide a raw material agitation method to enable it.

The present invention is to solve the problems of the prior art described above, using a solvent of water / ethanol = 50 / 50, wt%, drying, grinding, ethanol addition, extraction, filtration, concentration, freeze drying and crushing steps. It provides a method for extracting bokbunja by-product extract made of bokbunja and a composition containing the bokbunja by-product extract to provide a composition containing bokbunja extract capable of preventing or treating scalp care, dandruff prevention, and hair loss without irritation to the scalp. There is a purpose.

The present invention, in the raw material stirring method, the steps of preparing the raw material; It provides a raw material stirring method comprising the step of obtaining a composition containing the bokbunja by-product extract based on the stirred raw material.

According to another aspect of the present invention, the present invention provides a method for stirring raw materials, comprising: mixing water and ethanol at room temperature to extract bokbunja by-product extract and purifying for 24 hours; And agitating so that the mixed solution is evenly mixed through a stirring device, wherein the stirring device includes a base panel 90 provided on a lower surface of a plurality of support bars, and a stand 80 provided on the base panel 90. ) Fixedly installed at the bottom of the tank 10 with an outlet, and a rotating rod 20 provided to be able to move up and down along the inside of the tank 10 and rotate at the same time, and the rotating rod 20 A driving motor 30 for driving, an impeller 40 formed at a lower end of the rotating rod 20 to agitate the contents, and a mounting unit 100 mounted on the base panel 90, wherein the mounting unit 100, a lower body 110 under the base panel 90, a first central body 111 provided on an upper portion of the lower body 110, and. A 1-1 front-rear flow body 112 provided to be able to flow in one direction on the first central body 111 and through which the first support bar 1001 of the support bars is mounted vertically, and the first central body 111 ) It is provided so as to be able to flow back and forth from the top to the other side, and the second support bar (1002) of the support bar is mounted vertically through the first-2 front-rear fluid (113), and the first provided on the top of the first central body (111). 2 with the central body (120). On the second central body 120, a 2-1 front-rear fluid 121 is provided so as to be able to flow back and forth in one direction and the first support bar 1001 is mounted vertically, and on the second central body 120 It is provided so as to be able to flow back and forth in the other direction, and is located at the upper end of the 2-2 front-rear fluid 122 and the 2-1 front-rear fluid 121 to which the second support rod 1002 is mounted vertically. 2 The clothes of the 3-1 front-rear fluid 123 and the 2-2 front-rear fluid 122 are provided so as to be able to flow back and forth in one direction on the central body 120 and the first support rod 1001 is mounted vertically through the top and bottom. It is located at the end, is provided so as to flow back and forth on the second central body 120 to the other side, and includes a 3-2 forward and backward fluid 124 through which the second support rod 1002 is mounted vertically, and the second 1 The support rod 1001 includes a first-first-stage mode in which a pulling force is applied inward by the first-first front-rear fluid 112 and the second-first-rear fluid ( 121) in the second-stage mode, which is applied in the second stage, and in the second-stage mode, in which the 3-1 front-rear fluid (123) applies a third force that is pulled inwardly. The second support rod 1002 is fixed based on the 3-1 stage mode, and the second support rod 1002 includes a 1-2 stage mode in which a pulling force is applied inward by the backward movement of the 1-2 second front and rear fluid 113, and the In the 1-2 stage mode, the 2-2 stage mode is pulled inward by the reversing of the 2-2 front and rear fluid 122, and the 3-2 stage 2 is applied in the 2nd stage mode. It is fixed based on the 3-2 stage mode in which the force being pulled inward by the backward movement of the front and rear fluid 124 is applied in a third order, and the mounting unit 100 flows between the upper and lower sides in the lower body 110 The 1-1 vertical fluid 125 is in contact with the top surface of the 1-1 front and rear fluid 112 and one end of the 2-1 front and rear fluid 121 from the 1-1 vertical fluid 125 To be provided on the side The 1-1 extension body 126, the 1-2 up and down fluid 127 flowing between the upper and lower sides in the lower body 110, and the 1-2 in the 1-2 up and down fluid 127 It further includes a 1-2 extension body 128 provided on the side so as to be in contact with the upper surface of the front and rear fluid 113 and one end of the 2-2 front and rear fluid 122, and the 1-1 vertical fluid 125 The 1-1 extension body 126 depresses the 1-1 front-rear fluid body 112 based on the descent of, and the 1-2 extension body is based on the descent of the 1-2 up and down fluid body 127. 128 depresses the 1-2 second front-rear fluid 113, and the mounting unit 100 is located outside the 1-1 up-down fluid 125 in the lower body 110, and The 2-1 vertical fluid 131 flowing downward, the top surface of the 1-1 extension body 126 and the top surface of the 1-1 extension body 126 in the 2-1 vertical fluid body 131 The 2-1 extension body 132 provided on the side to be in contact with the end of the 3-1 front-rear fluid 123, and located outside the 1-2 vertical fluid body 127 in the lower body 110 And the upper surface of the 1-2 extension body 128 and the 1-2 extension body 128 in the 2-2 vertical fluid 133 flowing between the upper and lower fluids and the 2-2 vertical fluid 133 It provides a raw material stirring method further comprising a second-second extension body 134 provided on the side so as to be in contact with the upper surface of and the end of the 3-2 front and rear fluid 124.

According to another aspect of the present invention, the present invention provides a method for stirring raw materials, comprising: preparing raw materials; It provides a raw material stirring method comprising the step of obtaining a composition containing the cheonnyeoncho extract based on the stirred raw material.

According to another aspect of the present invention, the present invention includes the step of configuring a raw material stirring method to stir and homogenize a mixture of Cheonnyeoncho using a stirring device, wherein the stirring device includes a plurality of support rods provided on a lower surface thereof. The base panel 90 and the tank 10 fixedly installed on the stand 80 provided on the base panel 90 and having an outlet at the bottom, and the tank 10 can be moved up and down along the inside of the tank 10 And the rotating rod 20 provided to be rotated at the same time, the driving motor 30 for driving the rotating rod 20, the impeller 40 formed at the lower end of the rotating rod 20 to agitate the contents, and the Including a mounting unit 100 mounted on the base panel 90, the mounting unit 100, the lower body 110 under the base panel 90, and provided on the upper portion of the lower body 110 The first central body 111 and. A 1-1 front-rear flow body 112 provided to be able to flow in one direction on the first central body 111 and through which the first support bar 1001 of the support bars is mounted vertically, and the first central body 111 ) It is provided so as to be able to flow back and forth from the top to the other side, and the second support bar (1002) of the support bar is mounted vertically through the first-2 front-rear fluid (113), and the first provided on the top of the first central body (111). 2 with the central body (120). On the second central body 120, a 2-1 front-rear fluid 121 is provided so as to be able to flow back and forth in one direction and the first support bar 1001 is mounted vertically, and on the second central body 120 It is provided so as to be able to flow back and forth in the other direction, and is located at the upper end of the 2-2 front-rear fluid 122 and the 2-1 front-rear fluid 121 to which the second support rod 1002 is mounted vertically. 2 The clothes of the 3-1 front-rear fluid 123 and the 2-2 front-rear fluid 122 are provided so as to be able to flow back and forth in one direction on the central body 120 and the first support rod 1001 is mounted vertically through the top and bottom. It is located at the end, is provided so as to flow back and forth on the second central body 120 to the other side, and includes a 3-2 forward and backward fluid 124 through which the second support rod 1002 is mounted vertically, and the second 1 The support rod 1001 includes a first-first-stage mode in which a pulling force is applied inward by the first-first front-rear fluid 112 and the second-first-rear fluid ( 121) in the second-stage mode, which is applied in the second stage, and in the second-stage mode, in which the 3-1 front-rear fluid (123) applies a third force that is pulled inwardly. The second support rod 1002 is fixed based on the 3-1 stage mode, and the second support rod 1002 includes a 1-2 stage mode in which a pulling force is applied inward by the backward movement of the 1-2 second front and rear fluid 113, and the In the 1-2 stage mode, the 2-2 stage mode is pulled inward by the reversing of the 2-2 front and rear fluid 122, and the 3-2 stage 2 is applied in the 2nd stage mode. It is fixed based on the 3-2 stage mode in which the force being pulled inward by the backward movement of the front and rear fluid 124 is applied in a third order, and the mounting unit 100 flows between the upper and lower sides in the lower body 110 The 1-1 vertical fluid 125 is in contact with the top surface of the 1-1 front and rear fluid 112 and one end of the 2-1 front and rear fluid 121 from the 1-1 vertical fluid 125 To be provided on the side The 1-1 extension body 126, the 1-2 up and down fluid 127 flowing between the upper and lower from the lower body 110, and the 1-2 in the 1-2 vertical fluid 127 Further comprising a 1-2 extension body (128) provided in the side so as to contact the upper surface of the front and rear fluid (113) and one end of the 2-2 front and rear fluid (122), the 1-1 vertical fluid body (125) The 1-1 extension body 126 depresses the 1-1 front and rear fluid 112 based on the descent of the 1-2, and the 1-2 extension body is based on the descent of the 1-2 up and down fluid body 127. 128 depresses the 1-2 second front-rear fluid 113, and the mounting unit 100 is located outside the 1-1 vertical fluid 125 in the lower body 110, and the upper and The 2-1 vertical fluid 131 flowing downward, the top surface of the 1-1 extension body 126 and the top surface of the 1-1 extension body 126 in the 2-1 vertical fluid body 131 The 2-1 extension body 132 provided on the side to be in contact with the end of the 3-1 front-rear fluid 123, and located outside the 1-2 vertical fluid body 127 in the lower body 110 And the upper surface of the 1-2 extension body 128 and the 1-2 extension body 128 in the 2-2 vertical fluid 133 flowing between the upper and lower flow bodies 133 and the 2-2 vertical fluid 133 It provides a raw material agitation method further comprising a 2-2 extension body 134 provided on the side to be in contact with the upper surface and the end of the 3-2 front and rear fluid 124 of the.

According to the present invention, in performing agitation for an object, by optimizing the work environment at the site where the agitation is performed in the process of mass and repetitive input and agitation of the object By preventing exposure to shock and other negative factors, the occurrence of industrial accidents and occupational diseases of workers can be prevented. In addition, there is an effect of ensuring the seismic performance of the stirring means in the stirring process itself.

1 is a diagram showing a process of extracting bokbunja;
Figure 2 shows the scalp condition of a patient with hair loss symptoms before using a product containing the ingredients of the present invention,
3 shows the state of the scalp of a patient with hair loss symptoms after using the product containing the component of the present invention for 1 month.
Figure 4 is a perspective view showing a stirring device according to the present invention.
5 is a cross-sectional view showing a stirring device according to the present invention.
6 is a view showing the operating state of the stirring device according to the present invention.
7 to 9 are views schematically showing a stirring device according to another embodiment of the present invention.

- 실험결과에서 보듯이 복분자 부산물 중 주류 부산물(찌꺼기)를 이용하여 추출 방법 중 에탄올/물=50/50,wt% 에서 가장 좋은 추출을 얻을 수 있다.
- 특히, 복분자 주류 부산물에서 폴리페놀 수치가 제일 높음을 알 수 있으며, 이에 따라 두피케어 및 비듬방지에 탁월함을 알 수 있다.
3. 복분자 부산물 추출물 추출과정(제2 예)
1) 복분자 준비단계
(1) 냉동단계
재료(복분자, 오렌지, 양파) 각각을 약 -30℃의 온도로 30분간 급냉시키는 단계이다. 이 냉동단계에서는 재료가 동결되면서 냉동건조로 수분을 일부 제거된다.
(2) 분쇄단계
냉동된 재료 각각을 분쇄기로 분쇄하는 단계이다.
(3) 사과단계
재료 분쇄물 각각을 체(Sieve)로 걸러 일정 크기 이하의 입자로 선별하는 단계이다. 이 사과단계에서는 다음단계의 원활한 진행을 위해 선별된 재료 분쇄물은 그 입자가 48mesh 정도가 적당하다.
(4) 교반단계
재료 분쇄물의 입자가 고르게 섞이도록 교반하는 단계이다.
(5) 냉침단계
교반된 재료 분쇄물을 실온(18±2℃)에서 5~7일(day)간 냉침하는 단계이다. 실온에서 충분히 냉침되는 과정에서 재료 분쇄물은 수분이 녹으면서 침전물이 액체의 하부로 침전이 진행된다.
(6) 원심분리단계
냉침된 재료 각각을 원심분리기로 원심분리(1200~1500rpm, 1분~1분30초)하여 복분자추출액을 얻는 단계이다.
(7) 동결건조단계
원심분리된 추출액 각각을 -30℃의 온도로 급속 냉각시켜 동결건조시키는 단계이다. 동결건조된 추출물은 함수율이 약 5%정도로 건조된다.
(8) 분쇄단계
동결건조된 추출 고형물을 분쇄기로 분쇄하는 단계이다.
(9) 제균 및 사과단계
분쇄된 추출물을 살균하고 일정 크기 이하의 입자크기의 추출분말을 얻는 단계이다. 추출분말은 150~200mesh의 입자 크기를 갖는 것이 바람직하다.
2) 추출물 추출단계
물/에탄올=50/50wt%의 용매 3kg에 분쇄한 복분자분말을 10 ~50%을 혼합한다.
이후 혼합물 1kg에 정제수 5배수를 가하여 70℃에서 8시간 동안 침지한 후 저온에서 원심분리를 한 후 감압 농축하여 당유자 추출물을 추출한다.
4. 당유자 추출물 추출과정
1) 당유자 준비단계
(1) 냉동단계
먼저, 당유자의 과육과 껍질을 분리한다.
재료를 약 -30℃의 온도로 30분간 급냉시키는 단계이다. 이 냉동단계에서는 재료가 동결되면서 냉동건조로 수분을 일부 제거된다.
(2) 분쇄단계
냉동된 재료 각각을 분쇄기로 분쇄하는 단계이다.
(3) 사과단계
재료 분쇄물을 체(Sieve)로 걸러 일정 크기 이하의 입자로 선별하는 단계이다. 이 사과단계에서는 다음 단계의 원활한 진행을 위해 선별된 재료 분쇄물은 그 입자가 48mesh 정도가 적당하다.
(4) 교반단계
재료 분쇄물의 입자가 고르게 섞이도록 교반하는 단계이다.
(5) 냉침단계
교반된 재료 분쇄물을 실온(18±2℃)에서 5~7일(day)간 냉침하는 단계이다. 실온에서 충분히 냉침되는 과정에서 재료 분쇄물은 수분이 녹으면서 침전물이 액체의 하부로 침전이 진행된다.
(6) 원심분리단계
냉침된 재료 각각을 원심분리기로 원심분리(1200~1500rpm, 1분~1분30초)하여 당유자추출액을 얻는 단계이다.
(7) 제균 및 여과단계
원심분리된 추출액 각각을 살균시키고 여과하는 단계이다. 살균은 원심분리된 추출액을 복사열로 가열하여 이루어지고, 여과는 여과지로 화선지를 사용하여 원심분리된 추출액을 실온에서 3일(day) 정도 여과하여 이루어진다.
2) 추출물 추출단계
물/에탄올=50/50wt%의 용매 3kg에 분쇄한 당유자분말을 10 ~50%을 혼합한다.
이후 혼합물 1kg에 정제수 5배수를 가하여 70℃에서 8시간 동안 침지한 후 저온에서 원심분리를 한 후 감압 농축하여 당유자 추출물을 추출한다.
5. 조성물 제조과정
1) 혼합단계
상기 추출된 당유자 추출물에 복분자 부산물 추출물을 10 ~ 50% 혼합한다.
2) 교반단계
상기 혼합물을 고르게 섞이도록 교반하는 단계이다.
3) 냉침단계
교반된 재료 분쇄물을 실온(18±2℃)에서 5~7일(day)간 냉침하는 단계이다. 실온에서 충분히 냉침되는 과정에서 침전물이 액체의 하부로 침전이 진행된다.
4) 원심분리단계
냉침된 재료 각각을 원심분리기로 원심분리(1200~1500rpm, 1분~1분30초)하여 혼합액을 얻는 단계이다.
5) 동결건조단계
원심분리된 혼합액 각각을 -30℃의 온도로 급속 냉각시켜 동결건조시키는 단계이다. 동결건조된 추출물은 함수율이 약 5%정도로 건조된다.
6) 분쇄단계
동결건조된 추출 고형물을 분쇄기로 분쇄하는 단계이다.
7) 제균 및 사과단계
분쇄된 추출물을 살균하고 일정 크기 이하의 입자크기의 추출분말을 얻는 단계이다. 추출분말은 150~200mesh의 입자 크기를 갖는 것이 바람직하다.
상기 조성물에 의하면 두피에 자극을 주지 않으면서 두피케어를 할 수 있는 장점이 있다.
특히, 도 3에서 보는 바와 같이 본 조성물을 샴푸에 혼합하여 사용한 결과 탈모 방지, 비듬개선, 두피 가려움 개선, 발모 촉진 및 피지 제거 등 모발 건강의 유지에 도움되는 효과가 있음을 알 수 있다.
이때 상기 당유자 추출물 및 복분자 부산물 추출물 각각에 백출, 감초, 대황, 당귀, 작약, 방풍, 황금등을 수침엑스한 것을 50:50의 비율로 혼합하고 증류수를 첨가하여 3시간 정도 끊이면 알레르겐이 게거되는 데, 보다 상세하게는 추출한 당유자 추출물 및 복분자 부산물 추출물 각각에 800mg에 감초 600mg, 대황 500mg, 당귀 400mg, 작약 400mg, 방풍 500mg, 황금 600mg를 수침 엑스한 800mg을 혼합하여 1-3 시간 증류시키고 여과하여 여액을 감압농축시켜 알레르겐이 제거된 항산화물질을 함유한 당유자, 복분자 부산물 추출물을 제조할 수 있다.
그리고 상기 당유자 추출물 및 복분자 부산물 추출물 각각 80% 중량부에 숙지황, 산약, 산수유, 백복령, 목단피 및 택사를 각각 5 내지 8 % 중량부, 오미자, 천문동, 맥문동, 패모, 과루인, 행인, 반하, 지실, 길경, 황금, 황연을 각각 4 내지 6 % 중량부 및 감초를 2 내지 4 % 중량부를 더 포함할 수 있다.
이에 따른 조성물은 임상투여시에 경구 투여가 가능하며 일반적인 의약품 제제의 형태로 사용될 수 있다.
본 발명에 따른 조성물은 실제 임상투여시에 경구의 여러 가지 제형으로 투여될 수 있는데, 제제화 할 경우에는 보통 사용하는 충진제, 증량제, 결합제, 습윤제, 붕해제, 계면활성제 등의 희석제 또는 부형제를 사용하여 조제된다.
경구투여를 위한 고형제제에는 정제, 환제, 산제, 과립제, 캡슐제 등이 포함되며, 이한 고형제제는 본 발명에 따른 항염증 조성물에 적어도 하나 이상의 부형제 예를 들면, 전분, 칼슘카보네이트(Calcium carbonate), 수크로스(Sucrose) 또는 락토오스(Lactose), 젤라틴 등을 섞어 조제된다.
또한 단순한 부형제 이외에 마그네슘 스티레이트 탈크 같은 윤활제들도 사용된다. 경구를 위한 액상제제로는 현탁제, 내용액제, 유제, 시럽제 등이 해당되는데 흔히 사용되는 단순희석제인 물,리퀴드 파라핀 이외에 여러 가지 부형제, 예를 들면 습윤제, 감미제, 방향제,보존제 등이 포함될 수 있다.
그리고 탈모방지, 발모촉진, 육모, 비듬 제거 및 예방, 또는 흑모생성 촉진을 위하여 ⅰ) 세륨(Ce), 프라세오디뮴(Pr), 프로메튬(Pm), 유로퓸(Eu), 테르븀(Tb), 디스프로슘(Dy), 홀뮴(Ho), 에르븀(Er), 툴륨(Tm), 이테르븀(Yb), 스칸듐(Sc), 이트륨(Y), 란탄(La), 네오디뮴(Nd), 사마륨(Sm), 가돌리늄(Gd) 또는 루테튬(Lu)으로부터 선택되는 하나 이상의 원소, 또는 그의 염 또는 산화물과, ⅱ) 피토스테롤, 프로폴리스 또는 이들의 혼합물을 상기 당유자 추출물 및 복분자 부산물 추출물 각각 80% 중량부에 10 ~ 30% 중량부를 더 포함할 수 있다.
이들 혼합물 간의 상승작용에 의해 현저하게 우수한 TGF-β 발현 감소 및 VEGF 발현 촉진, 및 5α-리덕타아제(5α-reductase) 억제에 의한 탈모방지, 발모촉진, 육모, 비듬제거 및 예방 효과는 물론, 티로시나아제 활성 및 발현 촉진, 및 멜라닌 생성 촉진에 의한 흑모생성 촉진 효과를 나타낼 수 있다.
또한, 본 조성물은 인체에 무해하고 안전하며, 피부 흡수력이 높고 끈적이지 않아 피부 감촉이 우수하며, 두피에 부작용이 없다.
Ⅱ. 원재료 교반방법 1
한편, 전술한 내용을 기반으로 하는 원재료 교반방법은 원재료를 준비하는 단계; 교반된 원재료를 기반으로 복분자 부산물 추출물을 함유하는 조성물을 획득하는 단계를 포함한다.
또한, 전술한 내용을 기반으로 하는 원재료 원재료 교반방법은 복분자 부산물 추출물의 추출하기 위하여 상온의 물과 에탄올을 혼합하여 24시간 동안 정제하는 단계; 및 교반장치를 통해 혼합액을 고르게 섞이도록 교반하는 단계를 포함한다. 여기서 교반장치는 보다 구체적으로 후술하기로 한다.
Ⅲ. 천년초 추출물을 포함하는 나노에멀전 화장료 조성물
1. 제1 추출물인 천년초 추출물의 제조
(a) 천년초 줄기 및 천년초 열매를 포함하는 천년초 원재료를 35 내지 60℃의 물과 혼합한 후, 균질화 처리하여 천년초 혼합물을 제조하는 단계; 및 (b) 상기 천년초 혼합물을 여과하여 천년초 추출물을 제조하는 단계;를 포함하는 천년초 추출물의 제조방법을 제공한다.
상기 단계 (a)는 천년초 줄기 및 천년초 열매를 포함하는 천년초 원재료를 35 내지 60℃의 물과 혼합한 후, 균질화 처리하여 천년초 혼합물을 제조하는 단계로서, 본 단계에서는 적정 온도로 가열한 물을 천년초 원재료와 혼합한 후, 균질화 처리 장치를 이용해 균질화 처리하여 천년초 혼합물을 제조할 수 있다.
상기 천년초 혼합물의 제조는, 통상적인 식품 파쇄장치를 이용해 수행할 수 있며, 바람직하게는, 2마력 이상의 동력을 갖는 강한 모터와 양날의 칼이 구비된 파쇄장치를 이용하여 천년초를 물과 혼합한 상태에서 300 내지 400 메쉬의 평균 입자크기를 갖도록 파쇄하고, 가열된 물과의 혼합으로 인해 균질하게 천년초가 분쇄되어 혼합된 천년초 혼합물을 제조할 수 있다.
상기와 같은 방법으로 제조한 천년초 혼합물은 임펠러(impeller)가 구비된 혼합조에서 고속으로 교반하여 균질화하도록 구성하고, 천년초 분쇄물의 응집이 발생되지 않도록 물의 함량을 조절하여 천년초의 유효성분의 추출효율을 높일 수 있다. 이때, 상기 물은 추출수율을 향상시키고, 유효성분의 파괴가 유발되지 않도록 온도가 35 내지 60℃인 것을 사용하는 것이 바람직하며, 보다 바람직하게는, 40 내지 50℃의 물을 사용할 수 있다.
본 단계에서는, 천년초 원재료 5 내지 20kg을 35 내지 60℃의 물 70 내지 90kg과 혼합하여 상기 천년초 혼합물을 제조할 수 있다.
또한, 본 단계에서는 상기와 같이 상기 천년초 혼합물을 제조한 후, 상기 천년초 혼합물을 가압하는 단계를 추가로 포함하도록 구성하여 천년초의 식물 세포벽에 손상 또는 자극을 유도해 천년초에 포함된 각종 유효성분을 더욱 효과적으로 추출할 수 있다. 이때, 상기 천년초 추출물은 물을 용매로 포함하기 때문에, 천년초의 조직에 균일한 압력을 가해 가압에 의한 추출효율의 향상 효과가 더욱 높다.
구체적으로, 상기와 같은 고압 처리는 최근 식품분야에서 영양소와 비영양성의 식물성 화학물질 섭취에 있어 가장 효율적으로 활용될 수 있는 추출기술로서, 제조한 천년초 혼합물에 포함된 각종 유효성분의 파괴를 방지할 수 있도록, 외부와 기체 유통이 차단된 환경에서 30 내지 100MPa의 압력으로 가압하고, 40 내지 60 ℃의 온도로 가열하는 단계을 동시에 적용하여 천년초에 포함된 각종 유효성분을 단시간에 추출하는 것이 가능하며, 불순물이 거의 없어 고순도의 천년초 성분을 손쉽게 수득할 수 있는 장점을 갖는다.
상기 가압이 30MPa 미만의 압력을 진행될 경우, 가압효율이 떨어져 유효성분의 추출률이 크게 향상되지 않는 문제가 있고, 100MPa의 압력을 초과할 경우에는 온도 제어가 힘들고, 유효성분이 파괴될 수 있는 문제가 있다.
상기 단계 (b)는, 상기 천년초 혼합물을 여과하여 천년초 추출물을 제조하는 단계로서, 본 단계에서는 상기와 같은 추출방법으로 천년초의 유효성분을 다량 포함하는 천년초 혼합물을 300 내지 400 메쉬의 여과막으로 여과하여 천년초 추출물을 제조할 수 있다.
상기와 같은 방법으로 추출한 천년초 추출물은 천년초에 포함된 폴리페놀, 플라보노이드, 식이섬유, 비타민 C, 칼슘, 무기질, 아미노산 등의 유효성분을 다량 포함하여, 피부도입시 미백, 항산화, 피부보습, 주름 개선 등과 같은 피부 미용 개선효과를 가지며, 섭취시에도 성인병 예방, 항암 및 항산화 등에 유의적인 효과를 나타낼 수 있다.
하지만, 상기 천년초 추출물은 점액성을 띄는 펙틴계 고분자 다당류를 다량 포함한다.
구체적으로, 상기 천년초 추출물에 포함된 고분자 다당류는 글루코오스(glucose), 아라비노오스(arabinose), 갈락토오스(galactose), 자일로오스(xylose) 등의 환원당을 포함하며, 갈락투론산(galacturonic acid), 아라비노갈락탄(arabinogalactan), 람노오스(rhamnose) 등과 같은 다당류의 식이섬유소인 펙틴을 주요 성분으로 포함하는데, 이에 의해, 상기 천년초 추출물은 급격한 점성 변화 및 시간 의존성의 비관성 흐름 거동(non-Newtonian flow behavior)을 나타내는 강한 점도를 나타내게 된다. 즉, 상기 천년초 추출물은 상기와 같은 다당류를 포함함에 따라, 화장품 또는 식품 제조에 활용시 물에 과량 혼합될 경우, 부피가 급격히 팽창하거나, 과도한 점도를 나타내게 되어 화장품으로 사용시 기호성 및 사용감이 떨어지고, 화장품 또는 식품에 천년초 추출물을 과량 첨가하기 힘든 문제가 있어, 천년초 추출물의 도입으로 인한 효과가 다소 떨어진다는 문제가 있다.
특히, 상기 천년초 추출물을 이용해 화장품 또는 식품제조에 활용시, 천년초 추출물의 [0033] 농도가 5%(w/v) 이상으로 과량 첨가되면, 점도가 비례적으로 증가하고, 천년초 추출물의 용해도는 감소되어 천년초 추출물의 포함함량이 낮아, 우수한 생리활성 기능을 달성할 수 없게 된다.
이에 본 발명에서는, 종래에 사용되었던 미생물 발효단계 또는 산처리 단계 등과 달리 제조한 천년초 추출물에 방사선을 조사하여 천년초 추출물에 포함된 각종 고분자 다당류를 저분자화시켜, 천년초 추출물의 점도를 획기적으로 저감시킬 수 있다.
구체적으로, 상기와 같은 방사선 조사 단계은 감마선(γ)을 천년초 추출물에 조사하게 되면, 천년초 추출물에 물이 분해되어 자유 라디칼이 형성되고, 형성된 자유 라디칼이 연쇄반응을 일으며, 다당류의 분자의 변화를 유도하고, 다당류가 저분자화되어 천년초 추출물의 점성이 감소하게 된다. 상기와 같이 점성이 감소된 다당류를 포함하는 천년초 추출물은 화장품 또는 식품 제조에 활용시 첨가량을 획기적으로 증가시킬 수 있으며, 천년초 추출물에 포함된 각종 유효성분을 다량 담지할 수 있게되어, 종국에는 생리활성 기능이 보다 향상될 수 있다.
일례로, 상기 천년초 추출물에 5 내지 20 킬로그래이(kGy) 강도의 방사선을 3 내지 24시간 동안 조사하여 총 흡수량이 12 내지 20 킬로그래이(kGy)가 되도록 조절하여 다당류를 저분자화하고, 저분자화된 다당류를 포함하는 천년초 추출물을 한외여과막(Ultrafiltration Membrane)으로 여과하여 05 내지 5 kDa 범위의 분자량을 갖는 저분자화 처리된 다당류를 포함시킴에 따라 점도가 획기적으로 감소된 천년초 추출물을 수득할 수 있다.
상기와 같이 제조한 천년초 추출물은 종래에 알려진, 효소 처리, 산 처리 또는 가열 처리 등으로는 분해가 힘든 식이섬유인 펙틴을 효과적으로 분해하고, 유효성분의 파괴를 최소화할 수 있어 성분의 변성이 없을 뿐만 아니라, 각종 미생물을 효과적으로 제거하여 안정하게 사용할 수 있다.
나아가, 본 단계에서는 상기 방사선 조사 단계을 효율을 더욱 향상시키기 위해서, 800 내지 10,000ppm의 농도로 촉매를 첨가하도록 구성하여 다당류의 저분자화를 더욱 향상시켜, 천년초 추출물의 점도를 더욱 획기적으로 저감시킬 수 있다.
상기와 같은 역할을 하는 촉매는, 과산화물, 과초산(peroxyacetic acid), 이산화 티탄(TiO2) 등을 대표적인 예로 들수 있으며, 바람직하게는, 천년초 추출물에 방사선 조사시 물과 산소로 분해되어 친환경적인 과초산을 사용하여 방사선 조사에 의한 다당류의 저감효과를 더욱 향상시킬 수 있다.
상기와 같이, 방사선 조사 단계을 거친 천년초 추출물은 펙틴계 다당류가 저분자화되어 피부 도입 또는 섭취로 인한 다당류의 흡수력을 현저히 향상시킬 수 있으면서도, 점도를 낮추어 화장품 또는 식품 제조시 천년초 추출물을 과량 첨가하여도 우수한 기호성 및 물성을 갖는 천년초 화장품 또는 식품을 제조할 수 있게 된다.
상기한 바와 같은 본 발명에 따른 천년초 추출물의 제조방법에 따르면, 저온 고압 추출 및 방사선 조사의 복합단계을 이용하여, 천년초에 포함된 유효성분을 효과적으로 추출하고 천년초에 포함된 다당류를 저분자화시켜 점도를 저감시킴에 따라, 화장품 또는 식품 등의 제조시 천년초의 포함함량을 증가시켜 생리활성을 향상시킬 수 있는 우수한 물성의 천년초 추출물을 제조할 수 있다.
상기 방법으로 제조된 천년초 추출물은 점도가 낮아 10% 이상의 과량 첨가시에도 분산이 용이하고, 저분자화된 다당류를 포함함에 따라 생리활성이 우수하여 화장품 또는 식품 제조에 효과적으로 이용이 가능하다.
또한, 천년초 추출물의 분산을 향상시키고, 포함함량을 더욱 증가시키기 위해서, 1,2-헥산디올, 부틸렌글라이콜, 에칠헥산디올 등과 같은 용제를 추가적으로 포함할 수 있으며, 상기 용제를 1 내지 5 kg 혼합하여 천년초 추출물의 물성을 조절할 수 있다.
아울러, 상기 천년초 추출물은 비타민, 유분, 계면활성제, 미백제, 진정제, 킬레이트제, 색소, 지방산, 산화방지제, 향료, 방부제, 중화제 또는 이들의 혼합물로 이루어진 첨가제를 추가로 포함하도록 구성하여 다양한 목적에 맞는 적합한 화장품 제조에 활용이 가능하며, 상기 천년초 추출물은 세럼, 화장수, 오일, 크림, 에센스 등의 다양한 형태로 제조할 수 있다.
이하, 본 발명을 실시예를 들어 더욱 상세히 설명하도록 한다.
제시된 실시예는 본 발명의 구체적인 예시일 뿐이며, 본 발명의 범위를 제한하기 위한 것은 아니다.
<실시예 1>
도 1에 나타낸 바와 같은 방법을 이용해 천년초 추출물을 제조하였다.
먼저, 천년초 줄기 및 열매를 원재료로 하여 원재료를 물로 세척한 후, 세척한 원재료를 가시제거 장치에 투입하고, 40분 동안 가시를 제거하였다. 원재료에 가시를 제거하기 위해서, 브러쉬가 고정형성되고, 내부 수용공간에 진공을 형성시킬 수 있는 가시제거 장치를 사용하였다.
상기와 같은 방법으로 가시를 제거한 원재료를 물로 세척한 후, 컨베이어 밸트, 원형 터널 형태의 하우징 및 공기 분사부가 구비된 건조 장치에 통과시켜 습기를 제거하였으며, 습기가 제거된 원재료를 분쇄기에 공급하였다.
천년초 원재료가 공급된 분쇄기에 40℃의 물을 공급하여 15분 동안 천년초 원재료를 분쇄하여 천년초 혼합물을 제조하고, 제조한 천년초 혼합물을 혼합조에 공급한 후, 지속적으로 교반하여 균질한 혼합물을 제조하였으며, 혼합물의 제조시 40 내지 50℃의 온도로 가열하고, 점도를 확인하여 물을 추가 공급하여 응집 형성을 방지하였다. 상기 분쇄는 2마력의 고속회전이 가능한 모터 및 양날칼이 구비된 분쇄기를 사용하였고, 혼합물의 제조를 위해서 2개의 임펠러가 구비된 혼합조를 사용하였다.
상기와 같은 방법으로 제조된 천년초 혼합물을 고압균질기(coldisostatic press)에 공급한 후, 50℃의 온도 및 80MPa의 압력으로 고압처리를 수행하였으며, 고압처리한 천년초 혼합물을 여과하여 300 메쉬의 여과망으로 체걸음(sieving)하여 천년초 추출물을 수득하였다.
수득한 천년초 추출물을 Co-60 감마선 조사 시스템(point sourceAECL,IR-79)에 장입한 후, 시간당 10 kGy의 선량율로 10kGy의 총 흡수선량을 얻도록 25 MeV 변압형 전자가속기(ELV-8Model,EB Tech)를 이용하여 145mA의 빔전류로 전자선을 조사하였으며, 조사선의 흡수선량을 10kGy로 운전하여 다당류를 저분자화시키고, 저분자화처리한 천년초 추출물을 150 메쉬의 여과막으로 한외여과 처리하여 최종적으로 천년초 추출물을 수득하였다. 수득한 천년초 추출물은 변질을 방지하기 위해서, - 20℃의 냉장고에 보관하였다.
<실시예 2>
제조한 천년초 추출물에 과초산을 내지 3,000 ppm의 농도로 첨가한 후, 방사선을 조사하는 것을 제외하고는 실시예 1과 동일한 방법으로 천년초 추출물을 제조하였다.
<비교예 1>
실시예 1의 고압처리전 천년초 혼합물을 300 메쉬의 여과망으로 여과하여 천년초 추출물을 수득하였다.
<비교예 2>
실시예 1과 동일한 방법으로 고압처리한 천년초 혼합물을 300 메쉬의 여과망으로 여과하여 천년초 추출물을 수 득하였다.
<실험예 1> 유효성분의 성분 분석
제조한 천년초 추출물에 포함된 유효성분의 추출효율을 분석하기 위해서, 실시예 1 및 비교예 1에 따른 방법으로 제조한 천년초 추출물에 포함된 폴리페놀 및 플라보노이드의 성분분석을 수행하였으며, 그 결과를 하기의 표1에 나타내었다. 성분 분석은 막시모비치(Maksimovic) 방법을 수행하여 측정하였으며, 폴리페놀 성분 분석은 표준 물질로 갈릭산(garlic acid, Sigma Aldrich)를 사용하였고, 725 nm 파장의 빛으로 흡광도를 측정하여 평가하였고, 플라보노이드 성분 분석은 염화알루미늄 6화물을 천년초 추출물에 첨가하여 430nm 파장의 빛으로 흡광도를 측정하여 평가하였다.
폴리페놀(mg/g) 플라보노이드(mg/g) 실시예 1 479 ± 019 123 ± 007 비교표 1 336 ± 021 094 ± 011
표 1에 나타낸 바와 같이, 비교예 1에 따른 방법으로 제조한 천년초 추출물은 폴리페놀 및 플라보노이드의 함량에 비해, 고압 추출법으로 제조한 실시예 1의 천년초 추출물에 포함된 플라보노이드 및 폴리페놀은 포함함량이 보다 높은 것으로 확인되었으며, 이를 통해, 고압 추출에 의해 추출효율이 현저히 향상되었다는 사실을 확인할 수 있었다.<실험예 2> 점도 분석 및 분자량 분석제조한 천년초 추출물의 점도를 분석하기 위해서, 실시예 1, 실시예 2 및 비교예 1 및 2에 따른 방법으로 제조한 천년초 추출물의 항복력을 분석하였으며, 그 결과를 하기의 표 2에 나타내었다. 항복력 분석은 점도분석장치를 이용하여 20℃에서 농도에 따른 천년초 물추출물을 전단속도를 0 내지 300s-1까지 변화시키면서 전단응력을 측정하여 평가하였다.
항복력(Pa) 실시예 1 1639 ± 047 실시예 2 1327 ± 036 비교예 1 21,09 ± 049 비교예 2 2267 ± 053
표 2에 나타낸 바와 같이, 제조한 천년초 추출물은 모두 유동성 지수(n)가 0 < n <1의 조건을 보여 뉴턴의 법칙에 지배받지 않는 비관성 흐름 거동을 나타내는 것으로 확인되었으며, 특히, 비교예 2의 천년초 추출물의 경우가 비교예 1의 천년초 추출물에 비해 항복력이 가장 높은 것으로 확인되어 점도가 가장 높은 것을 확인할 수 있었다.반면에, 실시예 2의 천년초 추출물의 경우에는 실시예 1에 비하여도 항복력이 낮은 것으로 확인되었으며, 이와 같은 항복력 감소를 통해 점도가 현격히 감소하였음을 확인할 수 있었고, 비교예 1 및 2에 비해 점도가 현저히 낮아졌음을 확인할 수 있었다.상기한 바와 같은 결과를 통해, 방사선 조사를 통한 저분자화가 촉진되어 점도가 감소하였음을 확인할 수 있었으며, 촉매 첨가가 저분자화를 더욱 촉진시킬 수 있다는 사실을 확인할 수 있었다.또한, 실시예 1, 실시예 2 및 비교예 1 및 2에 따른 방법으로 제조한 천년초 추출물을 동결건조하여 천년초 분말을 제조하고, 제조한 분말을 10 내지 30g을 20℃의 물 100 mL과 혼합한 후, 5분 동안 격렬하게 교반하여 용해도를 확인한 결과, 비교예 1에 따른 방법으로 제조한 경우에는 10g의 천년초 분말을 첨가한 경우부터 응집이 확인되었고, 비교예 2에 따른 방법으로 제조한 경우에는 8g의 천년초 분말을 첨가한 경우부터 응집이 확인되었다. 상기와 같은 결과를 통해서, 실시예 1에 따른 방법으로 방사선 조사 처리를 통해, 고분자가 저분자화되어 점성이 현저히 감소하였음을 확인할 수 있었고, 실시예 2에 따른 방법으로 제조한 천년초 추출물은 용해도가 현격히 증가하였음을 확인할 수 있었다.
상기한 바와 같은 결과를 통해서, 본 발명에 따른 천년초 추출물의 제조방법으로 제조한 천년초 추출물은, 저온 고압 추출 및 방사선 조사의 복합 단계으로 인해, 천년초에 포함된 유효성분을 보다 높은 농도로 포함하고, 천년초에 포함된 다당류가 저분자화되어 점도가 낮아 화장품 또는 식품 등의 제조시 효과적으로 이용할 수 있다는 사실을 확인할 수 있었다.
2. 나노에멀져 화장품 조성물 제조
1. 1차 단계
a) 글리셀린 10 내지 20kg과 하이드로제네이트드레시틴 0.1 내지 3kg을 75℃에서 호모믹서를 이용하여 200rpm에서 10분간 용해
*b) 정제수 20 내지 30kg과 천년초 추출물 0.1 내지 1kg과, 에탄올 0.1kg 내지 50kg과, 다이프로릴렌글라이콜10 내지 20 kg을 상온에서 완전용해
c) 카프릴릭/카프릭트라이글리세라이드 20 내지 30kg에 네오펜틸글라이콜다이카프레이트 0.5 내지 내지lkg과, 스테아릭애씨드 0.5 내지 2%kg과, C12-20알킬글루코사이드 0.1 내지 1kg과, C14-22알코올 0.1 내지 1kg을 75℃에서 호모믹서를 이용하여 1500rpm에서 5분간 용해
2. 2차 단계
상기 1차 단계에서 제조된 추출물을 High prossure Homogenizer기기를 이용하여 1 내지 3회 통과시켜 150 내지 200나노미터가 되도록 제조
3. 3차 단계
상기 2차 단계을 통하여 제조된 혼합물에 50℃로 가열된 1,2-헥산디올 1 내지 2k을 혼합
4. 4차 고정
상기 제1차 단계에서 제조된 제조물 a 및 c 및 제3차 단계에서 제조된 혼합물을 혼합하여 나노에멀젼 화장료 조성물을 제조
3. 나도에멀젼 화장료 조성물 입도 분석 보고서
1. 실험방법
시료를 증류수로 1000~2000배 희석하여 진행하였다. Malvern Zetasizer Nano S90를 이용하여 입자분석을 실시하였다.
2. 실험결과
실험결과는 아래와 같다. 화장품 A는 A-1, A-2, A-3모두 비슷한 입자 사이즈를 보였으며 입자 크기는 260nm정도로 측정되었다. 화장품 B의 경우 B-3의 입자 사이즈가 141.2nm로 가장 작은 사이즈를 보였다. 또한 PdI값이 0.055로 분산도도 낮아 입자 크기가 고르게 제조된 것을 확인하였다.
1) A-1

2) A-2

3) A-3

4) B-1

5) B-2

6) B-3

본 발명의 화장료조성물은 기초화장(스킨, 로션, 크림, 에센스, 아이크림 등) 후 눈, 입가의%잔주름 부위나 여드름 흔적이 있는 부위에 01g - 02g 정도의 내용물을 덜어내어 피부의 요철부위를 메꾸어 주듯이 꼭꼭눌러 발라준 후 손가락을 이용하여 두드리듯 마무리하여 완전히 건조할 때까지 기다린 후 필요에 따라 색조화장을 한다. 이 때 피부의 요철 부위가 약간 깊은 경우에는 얇게 1분 간격으로 2-3회 연속적으로 바르고 완전히 마른 후 필요하다면 색조 화장하는 것이 바람직하다.
Ⅳ. 원재료 교반방법 2
한편, 전술한 내용을 기반으로 하는 원재료 교반방법은 원재료를 준비하는 단계; 교반된 원재료를 기반으로 천년초 추출물분자 부산물 추출물을 함유하는 조성물을 획득하는 단계를 포함할 수 있다. 또한, 전술한 내용을 기반으로 하는 원재료 교반방법은 교반장치를 이용하여 천년초 혼합물을 교반하여 균질화하도록 구성하는 단계를 포함하며 천년초 추출물의 제조할 수 있다.
이하에서는 전술한 구성적 특징중 교반을 수행하기 위한 교반장치에 대해서 설명하기로 한다. 도 4 내지 도 6을 참조하면 교반장치는 스탠드(80)에 고정설치되며 하부에 배출구(12)가 형성된 탱크(10)와, 상기 탱크(10)의 내부를 상하방향으로 승강하고 동시에 회전이 가능하도록 구비된 회전봉(20)과, 상기 회전봉(20)을 구동시키는 구동모터(30)와, 상기 회전봉(20)의 하단부에 형성되어 내용물을 교반시키는 임펠러(40)로 이루어진다. 탱크(10)는 상방이 개방되고 바닥은 중앙의 배출구(12)를 중심으로 타원형으로 요입된다. 상기 탱크(10)는 부식을 방지하기 위하여 스테인레스(Stainless)스틸로 이루어지는 것이 바람직하다. 상기 탱크(10)에서 혼합이 완료된 대상물(예 : 혼합액 등)은 배출구(12)를 통하여 외부로 배출된다. 회전봉(20)은 탱크(10)의 내부를 상항방향으로 승강이 가능하도록 구비된다. 따라서 상기 회전봉(20)의 일측에는 상기 회전봉(20)을 안내하도록 승강가이드(82)가 설치된다. 상기 회전봉(20)의 상부에는 상기 회전봉(20)을 회전시키는 구동모터(30)가 구비된다. 상기 회전봉(20)은 구동모터와 연결되는 상부회전봉(22)과 상기 임펠러(40)가 설치되는 하부회전봉(24)으로 구비된다. 여기서 상기 상부회전봉(22)과 상기 하부회전봉(24) 사이에는 임펠러(40)의 교환이 용이하도록 축이음(26)에 의해 연결된다. 구동모터(30)는 상기 회전봉(20)의 상부에 구비되며 전원을 인가하여 회전력을 발생시킨다. 상기 구동모터(30)는 상기 회전봉(20)과의 사이에 회전속도를 감속시키고 토오크를 향상시키기 위해서 감속기(32)가 설치된다. 임펠러(40)는 회전봉(20)의 하단부에 형성되며 임펠러(40)의 블레이드면(42)은 임펠러(40)의 회전에 의해 대상물이 하방으로 유동되도록 회전봉(20)의 회전방향으로 하향 경사지게 구비되는 것이 바람직하다. 여기서 상기 임펠러(40)의 블레이드면(42)은 45°로 하향경사지게 구비되는 것이 더욱 바람직하다. 또한 상기 임펠러(40)의 단부에는 대상물의 흐름이 반경방향으로 이탈하지 않도록 상기 회전봉(20)을 중심으로 회전방향에 접선으로 별도의 가이드(44)가 형성되는 것이 바람직하다.
도 7 내지 도 9는 본 발명의 다른 실시예에 따른 교반장치를 개략적으로 도시한 도면들이다. 이하에서는 전술한 내용들을 기반으로 하되 구성적 특징이 있는 부분을 중심으로 설명하기로 한다. 도 7 내지 도 9를 참조하면, 상기 교반장치는, 다수의 지지봉이 하면에 구비되는 베이스패널(90)과, 상기 베이스패널(90)상에 구비되는 스탠드(80)에 고정설치되며 하부에 배출구가 형성된 탱크(10)와, 상기 탱크(10)의 내부를 따라 상하방향으로 승강이 가능하고 동시에 회전이 가능하도록 구비된 회전봉(20)과, 상기 회전봉(20)을 구동시키는 구동모터(30)와, 상기 회전봉(20)의 하단부에 형성되어 내용물을 교반시키는 임펠러(40)와, 상기 베이스패널(90)에 장착되는 장착유닛(100)을 포함한다.
여기서 상기 장착유닛(100)은, 상기 장착유닛(100)은, 상기 베이스패널(90) 하부의 하방본체(110)와, 상기 하방본체(110)의 상부에 구비되는 제1 중앙본체(111)와. 상기 제1 중앙본체(111)상에서 일방으로 전후유동 가능하도록 구비되며 상기 지지봉 중 제1 지지봉(1001)이 상하 관통하여 장착되며,장착된 상기 제1 지지봉(1001)을 수평방향으로 당기거나 밀어 가압 고정하기위한 제1-1 전후유동체(112)와, 상기 제1 중앙본체(111)상에서 타방으로 전후유동 가능하도록 구비되며 상기 지지봉 중 제2 지지봉(1002)이 상하 관통하여 장착되며, 장착된 상기 제2 지지봉(1002)을 수평방향으로 당기거나 밀어 가압 고정하기위한 제1-2 전후유동체(113)와, 상기 제1 중앙본체(111)의 상부에 구비되는 제2 중앙본체(120)와. 상기 제2 중앙본체(120)상에서 일방으로 실린더모듈 기반으로 슬라이딩방식으로 전후방향으로 구동되며 상기 제1 지지봉(1001)이 상하 관통하여 장착되며, 장착된 상기 제1 지지봉(1001)을 수평방향으로 당기거나 밀어 가압 고정하기위한 제2-1 전후유동체(121)와, 상기 제2 중앙본체(120)상에서 타방으로 실린더모듈 기반으로 슬라이딩방식으로 전후방향으로 구동되며 상기 제2 지지봉(1002)이 상하 관통하여 장착되며, 장착된 상기 제2 지지봉(1002)을 수평방향으로 당기거나 밀어 가압 고정하기위한 제2-2 전후유동체(122)와, 상기 제2-1 전후유동체(121)의 상단에 위치되어, 상기 제2 중앙본체(120)상에서 일방으로 실린더모듈 기반으로 슬라이딩방식으로 전후방향으로 구동되며 상기 제1 지지봉(1001)이 상하 관통하여 장착되며, 장착된 상기 제1 지지봉(1001)을 수평방향으로 당기거나 밀어 가압 고정하기위한 제3-1 전후유동체(123)와, 상기 제2-2 전후유동체(122)의상단에 위치되어, 상기 제2 중앙본체(120)상에서 타방으로 실린더모듈 기반으로 슬라이딩방식으로 전후방향으로 구동되며 상기 제2 지지봉(1002)이 상하 관통하여 장착되며, 장착된 상기 제2 지지봉(1002)을 수평방향으로 당기거나 밀어 가압 고정하기위한 제3-2 전후유동체(124)를 포함할 수 있다.
여기서, 상기 제1 지지봉(1001)은, 상기 제1-1 전후유동체(112)에 의하여 내측으로 당겨짐 힘이 가해지는 제1-1단모드와, 상기 제1-1단모드에서 상기 제2-1 전후유동체(121)에 의하여 내측으로 당겨짐힘이 2차로 가해지는 제2-1단모드와, 상기 제2-1단모드에서 상기 제3-1 전후유동체(123)에 의하여 내측으로 당겨짐힘이 3차로 가해지는 제3-1단모드에 기반하여 고정될 수 있다.
상기 제2 지지봉(1002)은, 상기 제1-2 전후유동체(113)의 후진에 의하여 내측으로 당겨짐 힘이 가해지는 제1-2단모드와, 상기 제1-2단모드에서 상기 제2-2 전후유동체(122)의 후진에 의하여 내측으로 당겨짐힘이 2차로 가해지는 제2-2단모드와, 상기 제2-2단모드에서 상기 제3-2 전후유동체(124)의 후진에 의하여 내측으로 당겨짐힘이 3차로 가해지는 제3-2단모드에 기반하여 고정될 수 있다.
또한, 상기 장착유닛(100)은, 상기 하방본체(110)에서 실린더모듈을 기반으로 승강 또는 하강 유동되는 제1-1 상하유동체(125)와, 상기 제1-1 상하유동체(125)에서 상기 제1-1 전후유동체(112)의 상면과 상기 제2-1 전후유동체(121)의 일단에 접하도록 측면으로 구비되는 제1-1 연장체(126)와, 상기 하방본체(110)에서 실린더모듈을 기반으로 승강 또는 하강 유동되는 제1-2 상하유동체(127)와, 상기 제1-2 상하유동체(127)에서 상기 제1-2 전후유동체(113)의 상면과 상기 제2-2 전후유동체(122)의 일단에 접하도록 측면으로 구비되는 제1-2 연장체(128)를 포함할 수 있다.
여기서, 상기 제1-1 상하유동체(125)의 하강에 기반하여 상기 제1-1 연장체(126)는 상기 제1-1 전후유동체(112)를 내리누르며, 상기 제1-2 상하유동체(127)의 하강에 기반하여 상기 제1-2 연장체(128)는 상기 제1-2 전후유동체(113)를 내리누를 수 있다. 상기 장착유닛(100)은, 상기 하방본체(110)에서 상기 제1-1 상하유동체(125)의 외측에 위치되며 승강 또는 하강 유동되는 제2-1 상하유동체(131)와, 상기 제2-1 상하유동체(131)에서 상기 제1-1 연장체(126)의 상면과 상기 제1-1 연장체(126)의 상면 및 상기 제3-1 전후유동체(123)의 단부에 접하도록 측면으로 구비되는 제2-1 연장체(132)와, 상기 하방본체(110)에서 상기 제1-2 상하유동체(127)의 외측에 위치되며 승강 또는 하강 유동되는 제2-2 상하유동체(133)와, 상기 제2-2 상하유동체(133)에서 상기 제1-2 연장체(128)의 상면과 상기 제1-2 연장체(128)의 상면 및 상기 제3-2 전후유동체(124)의 단부에 접하도록 측면으로 구비되는 제2-2 연장체(134)를 포함할 수 있다.
상기 제2-1 상하유동체(131)의 하강에 기반하여 상기 제2-1 연장체(132)는 상기 제1-1 연장체(126)의 상면과 상기 제1-1 연장체(126)의 상면을 내리누르며, 상기 제2-2 상하유동체(133)의 하강에 기반하여 상기 제2-2 연장체(134)는 상기 제1-2 연장체(128)의 상면과 상기 제1-2 연장체(128)의 상면을 내리누르며, 상기 제1-1 연장체(126) 및 상기 제1-2 연장체(128)는 상기 제1-1 상하유동체(125)와 상기 제1-2 상하유동체(127)상에 내장 설치된 모터를 기반으로서 각각 수직방향 축회전 구동되며, 상기 제2-1 연장체(132) 및 상기 제2-2 연장체(134)는 상기 제2-1 상하유동체(131)와 상기 제2-2 상하유동체(133)상에 내장 설치된 모터를 기반으로서 각각 수직방향 축회전 구동된다. 이러한 수직방향 축회전되는 구성부들은 사각형상으로 구비되며, 축회전을 기반으로 인접하여 접촉되는 구성들을 가압하여 눌러 고정시키는 고정힘을 발생시킬 수 있게된다.
상기 교반장치는, 제1 교반장치(1)와 제2 교반장치(2)로 복수 구비되며, 상기 제1 교반장치(1)의 하방본체(110)와 상기 제2 교반장치(2)의 제2 방본체(110) 사이에는 상기 하방본체(110)와 상기 제2 하방본체(110)를 연결시키는 제1 연결모듈(210)이 구비되며, 상기 하방본체(110)의 외측에는 제2 연결모듈(220)이 구비되며, 상기 제2 하방본체(110)의 외측에는 제3 연결모듈(230)이 구비되며, 상기 제1 교반장치(1) 및 상기 제2 교반장치(2)의 하방에 상기 제1 연결모듈(210) 내지 상기 제3 연결모듈(230)을 실린더모듈, 엑츄에이터 등을 기반으로 슬라이딩방식으로 좌우간에 당기거나 미는 방식으로 구동시키기 위한 베이스구동체(240)가 구비된다.
상기 제1 연결모듈(210)은, 상기 베이스구동체(240)에서 상하 유동 가능하게 구비되는 제1 기둥체(211)와, 상기 제1 기둥체(211)의 상단부에 구비되는 제1 구동영역부(212)와, 상기 제1 구동영역부(212)의 일측에서 상기 하방본체(110)의 일단부를 외삽 끼움방식으로 결속되는 제1-1 끼움부(213)와, 상기 제1 구동영역부(212)의 타측에서 상기 제2 하방본체(110)의 일단부를 외삽 끼움방식으로 결속되는 제1-2 끼움부(214)가 구비된다. 상기 제2 연결모듈(220)은, 상기 베이스구동체(240)에서 상하 유동 가능하게 구비되는 제2 기둥체(221)와, 상기 제2 기둥체(221)의 상단부에 구비되는 제2 구동영역부(222)와, 상기 제2 구동영역부(222)의 일측에서 상기 하방본체(110)의 타단부를 외삽 끼움방식으로 결속되는 제2 끼움부(223)가 구비되된다.
상기 제3 연결모듈(230)은, 상기 베이스구동체(240)에서 상하 유동 가능하게 구비되는 제3 기둥체(231)와, 상기 제3 기둥체(231)의 상단부에 구비되는 제3 구동영역부(232)와, 상기 제3 구동영역부(232)의 일측에서 상기 하방본체(110)의 타단부를 외삽 끼움방식으로 결속되는 제3 끼움부(233)가 구비되며, 상기 제1-1 끼움부(213)와 상기 제2 끼움부(223)는 상호간에 반대방향으로 회동되어 상기 하방본체(110)에 대한 회전 고정힘을 발생시키며, 상기 제1-2 끼움부(214)와 상기 제3 끼움부(233)는 상호간에 반대방향으로 회동되어 상기 제2 하방본체(110)에 대한 회전 고정힘을 발생시키며, 상기 제1 기둥체(211) 내지 상기 제3 기둥체(231)는 상기 베이스구동체(240)에서 좌우간에 슬라이딩방식으로 왕복유동 가능하게 구비된다.
이처럼 본 발명은 기재된 실시예에 한정되는 것이 아니고, 본 발명의 사상 및 범위를 벗어나지 않고 다양하게 수정 및 변형할 수 있음은 이 기술의 분야에서 통상의 지식을 가진 자에게 자명하다. 따라서 그러한 수정예 또는 변형예들은 본 발명의 청구범위에 속한다고 하여야 할 것이다. 한편, 전술한 전후유동 또는 상하 승강에 따른 구동방식은 실린더 모듈, Acturator(액츄에이터), 기타 적용 가능한 공지의 유동수단 등을 기반으로 구동하도록 구현되는 것으로서 신규한 동작방식의 고도화된 기술까지 요하지 않음은 통상의 당업자에게 자명하다 Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those of ordinary skill in the art may easily implement the present invention.
However, since the description of the present invention is merely an embodiment for structural or functional description, the scope of the present invention should not be construed as being limited by the embodiments described in the text.
For example, since the embodiments may be modified in various ways and may have various forms, the scope of the present invention should be understood as including equivalents capable of realizing the technical idea.
In addition, since the object or effect presented in the present invention does not mean that a specific embodiment should include all or only such effects, the scope of the present invention should not be understood as being limited thereto.
In the present specification, the present embodiment is provided to complete the disclosure of the present invention, and to fully inform a person of ordinary skill in the art to which the present invention belongs. And the invention is only defined by the scope of the claims.
Therefore, in some embodiments, well-known components, well-known operations, and well-known techniques have not been described in detail in order to avoid obscuring interpretation of the present invention.
Meanwhile, the meaning of the terms described in the present invention is not limited to the dictionary meaning, and should be understood as follows.
All terms used herein have the same meaning as commonly understood by one of ordinary skill in the field to which the present invention belongs, unless otherwise defined.
Terms defined in a commonly used dictionary should be construed as having the meanings of the related technology, and cannot be construed as having an ideal or excessively formal meaning unless explicitly defined in the present invention.
Ⅰ. Method for extracting bokbunja by-product extract and composition containing the bokbunja by-product extract
Hereinafter, a process for preparing a composition containing the extract of bokbunja by-product is as follows.
1. Solvent extraction process
1) Purification step
This is a step of purifying for 24 hours by mixing water and ethanol at room temperature.
2) Stirring step
This is the step of stirring so that the mixed solution is evenly mixed.
3) Cold settling step
This is a step of cold-settling the agitated pulverized material at room temperature (18±2°C) for 5 to 7 days. In the process of sufficiently cold precipitation at room temperature, the precipitate proceeds to the bottom of the liquid.
4) Centrifugation step
Each of the cold-precipitated materials is centrifuged with a centrifuge (1200 to 1500 rpm, 1 minute to 1 minute and 30 seconds) to extract the solvent.
2. Extraction process of bokbunja by-product extract (Example 1)
(1) drying step
Completely dry in a dry oven (60℃) by taking the skins excluding the fruit of sugar citron.
(2) grinding step
Completely grind using a blender after drying
(3) ethanol addition step
After weighing the sample, add 50% EtOH 14 times the volume
(4) Extraction step
Mix thoroughly and extract (24h)
(5) filtration step
The extracted extract is filtered using a vacuum filter.
(6) Concentration step
Vacuum rotary concentration (40℃, 40rpm)7
(7) drying step
After concentration, dry using a freeze dryer
<Test result>

-As shown in the experimental results, the best extraction can be obtained in ethanol/water=50/50,wt% of the extraction method using liquor by-products (seeds) among by-products of bokbunja.
-In particular, it can be seen that the polyphenol level is the highest in bokbunja liquor by-products, and accordingly, it is excellent in scalp care and dandruff prevention.
3. Extraction process of bokbunja by-product extract (2nd example)
1) Preparation stage of bokbunja
(1) freezing step
In this step, each of the ingredients (bokbunja, orange, onion) is quenched at a temperature of about -30℃ for 30 minutes. In this freezing step, while the material is frozen, some moisture is removed by freeze drying.
(2) grinding step
In this step, each of the frozen materials is pulverized with a grinder.
(3) apology stage
In this step, each pulverized material is filtered through a sieve and sorted into particles of a certain size or less. In this apple stage, the particle size of the selected pulverized material is appropriate for the smooth progress of the next stage.
(4) Stirring step
This is a step of stirring so that the particles of the pulverized material are evenly mixed.
(5) Cold settling step
This is a step of cold-settling the agitated pulverized material at room temperature (18±2°C) for 5 to 7 days. In the process of sufficiently cold sedimentation at room temperature, the material pulverized material dissolves moisture and the precipitate proceeds to the bottom of the liquid.
(6) Centrifugation step
This is a step to obtain a bokbunja extract by centrifuging each of the chilled materials with a centrifuge (1200~1500rpm, 1 minute to 1 minute 30 seconds).
(7) Freeze drying step
In this step, each of the centrifuged extracts is rapidly cooled to a temperature of -30°C and freeze-dried. The lyophilized extract is dried to a moisture content of about 5%.
(8) grinding step
This is a step of pulverizing the lyophilized extracted solid with a grinder.
(9) Sterilization and apple stage
This is a step of sterilizing the pulverized extract and obtaining an extract powder having a particle size of less than a certain size. It is preferable that the extracted powder has a particle size of 150 to 200 mesh.
2) Extract extraction step
Mix 10-50% of crushed bokbunja powder in 3 kg of a solvent of water/ethanol=50/50wt%.
Thereafter, 5 times of purified water was added to 1 kg of the mixture, immersed at 70° C. for 8 hours, centrifuged at low temperature, and concentrated under reduced pressure to extract the sugar citron extract.
4. Extraction process of sugar citron extract
1) preparation stage
(1) freezing step
First, separate the flesh and skin of the sugar citron.
This is a step of quenching the material to a temperature of about -30°C for 30 minutes. In this freezing step, while the material is frozen, some moisture is removed by freeze drying.
(2) grinding step
In this step, each of the frozen materials is pulverized with a grinder.
(3) apology stage
In this step, the pulverized material is filtered through a sieve and sorted into particles of a certain size or less. In this apple step, the particle size of the selected material pulverized material is appropriate for the smooth progress of the next step.
(4) Stirring step
This is a step of stirring so that the particles of the pulverized material are evenly mixed.
(5) Cold settling step
This is a step of cold-settling the agitated pulverized material at room temperature (18±2°C) for 5 to 7 days. In the process of sufficiently cold sedimentation at room temperature, the material pulverized material dissolves moisture and the precipitate proceeds to the bottom of the liquid.
(6) Centrifugation step
This is a step to obtain sugar citron extract by centrifuging each of the cold-precipitated materials with a centrifuge (1200 to 1500 rpm, 1 minute to 1 minute 30 seconds).
(7) Sterilization and filtration step
This is a step of sterilizing and filtering each of the centrifuged extracts. Sterilization is performed by heating the centrifuged extract with radiant heat, and filtration is performed by filtering the centrifuged extract at room temperature for about 3 days using Hwaseon paper as a filter paper.
2) Extract extraction step
10-50% of the pulverized sugar citron powder is mixed with 3 kg of a solvent of water/ethanol=50/50wt%.
Thereafter, 5 times of purified water was added to 1 kg of the mixture, immersed at 70° C. for 8 hours, centrifuged at low temperature, and concentrated under reduced pressure to extract the sugar citron extract.
5. Composition manufacturing process
1) Mixing step
10-50% of bokbunja by-product extract is mixed with the extracted sugar citron extract.
2) Stirring step
This is a step of stirring so that the mixture is evenly mixed.
3) Cold settling step
This is a step of cold-settling the agitated pulverized material at room temperature (18±2°C) for 5 to 7 days. In the process of sufficiently cold precipitation at room temperature, the precipitate proceeds to the bottom of the liquid.
4) Centrifugation step
This is a step to obtain a mixed solution by centrifuging each of the cold-precipitated materials with a centrifuge (1200 to 1500 rpm, 1 minute to 1 minute 30 seconds).
5) Freeze drying step
In this step, each of the centrifuged mixtures is rapidly cooled to a temperature of -30°C and freeze-dried. The lyophilized extract is dried to a moisture content of about 5%.
6) Crushing step
This is a step of pulverizing the lyophilized extracted solid with a grinder.
7) Sterilization and apple stage
This is a step of sterilizing the pulverized extract and obtaining an extract powder having a particle size of less than a certain size. It is preferable that the extracted powder has a particle size of 150 to 200 mesh.
According to the composition, there is an advantage in that scalp care can be performed without irritation to the scalp.
In particular, as shown in FIG. 3, as a result of using the composition mixed with shampoo, it can be seen that there is an effect of helping to maintain hair health such as preventing hair loss, improving dandruff, improving scalp itching, promoting hair growth, and removing sebum.
At this time, the mixture of Baekchul, licorice, rhubarb, angelica, peony, windbreak, gold, etc. to each of the sugar citron extract and bokbunja by-product extract in a 50:50 ratio, added distilled water, and boiled for about 3 hours. In more detail, 800mg of licorice 600mg, rhubarb 500mg, angelica 400mg, peony 400mg, windbreak 500mg, gold 600mg in each of the extracted sugar citron extract and bokbunja by-product extract were mixed and distilled for 1-3 hours. By filtration, the filtrate is concentrated under reduced pressure to prepare an extract of sugar citron and bokbunja by-products containing antioxidants from which allergens have been removed.
And 5 to 8% by weight of Sukjihwang, Sanyak, Sansuyu, Baekbokryeong, Mokdanpi, and Taeksa, respectively, in 80% parts by weight of the sugar citron extract and bokbunja by-product extract, Omija, Cheonmundong, Maekmundong, Pamo, Guaruin, Passengin, Banha It may further include 4 to 6% by weight of jisil, gilgyeong, gold, and yellowyeon, respectively, and 2 to 4% by weight of licorice.
The composition according to this can be administered orally at the time of clinical administration and can be used in the form of a general pharmaceutical formulation.
The composition according to the present invention can be administered in various oral dosage forms at the time of actual clinical administration. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants, and surfactants are used. It is prepared.
Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and these solid preparations include at least one excipient, such as starch, calcium carbonate, in the anti-inflammatory composition according to the present invention. , Sucrose (Sucrose) or lactose (Lactose), is prepared by mixing, such as gelatin.
In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral use include suspensions, liquid solutions, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as wetting agents, sweetening agents, fragrances, and preservatives may be included. .
And to prevent hair loss, promote hair growth, remove and prevent hair growth, dandruff, or promote black hair production i) cerium (Ce), praseodymium (Pr), promethium (Pm), europium (Eu), terbium (Tb), dysprosium (Dy) ), holmium (Ho), erbium (Er), thulium (Tm), ytterbium (Yb), scandium (Sc), yttrium (Y), lanthanum (La), neodymium (Nd), samarium (Sm), gadolinium (Gd) ) Or at least one element selected from lutetium (Lu), or a salt or oxide thereof, and ii) phytosterol, propolis, or a mixture thereof, to 80% by weight of the sugar citron extract and bokbunja by-product extract, respectively, by 10 to 30% by weight May contain more wealth.
Remarkably excellent TGF-β expression reduction and VEGF expression promotion by synergy between these mixtures, and prevention of hair loss by inhibition of 5α-reductase, promotion of hair growth, hair growth, dandruff removal and prevention effects, as well as It may exhibit an effect of promoting tyrosinase activity and expression, and promoting melanogenesis by promoting melanogenesis.
In addition, the composition is harmless and safe to the human body, has high skin absorption power and is not sticky, so it has excellent skin texture and has no side effects on the scalp.
Ⅱ. Raw material stirring method 1
On the other hand, the raw material stirring method based on the above-described content comprises the steps of preparing raw materials; It includes the step of obtaining a composition containing the bokbunja by-product extract based on the stirred raw material.
In addition, a method of stirring raw materials based on the above description includes the steps of mixing water and ethanol at room temperature to extract bokbunja by-product extract and purifying for 24 hours; And agitating the mixed solution to be evenly mixed through a stirring device. Here, the stirring device will be described in more detail later.
Ⅲ. Nanoemulsion cosmetic composition containing Cheonnyeoncho extract
1. Preparation of the first extract, Cheonnyeoncho extract
(a) mixing the raw materials of Cheonnyeoncho, including the stems of Cheonnyeoncho and the fruits of Cheonnyeoncho, with water at 35 to 60°C, and homogenizing to prepare a Cheonnyeoncho mixture; And (b) filtering the mixture of cheonnyeoncho to prepare a cheonnyeoncho extract; provides a method for producing a cheonnyeoncho extract comprising.
The step (a) is a step of preparing a mixture of cheonnyeoncho by mixing raw materials including cheonnyeoncho stalks and cheonnyeoncho fruit with water at 35 to 60°C, and then homogenizing it to prepare a mixture of cheonnyeoncho. After mixing with the raw material, it can be homogenized using a homogenization treatment device to prepare a cheonnyeoncho mixture.
The preparation of the cheonnyeoncho mixture can be carried out using a conventional food crushing device, preferably, a state in which cheonnyeoncho is mixed with water using a crushing device equipped with a double-edged knife and a strong motor having a power of 2 horsepower or more It is crushed to have an average particle size of 300 to 400 mesh in, and by mixing with heated water, the cheonnyeoncho is homogeneously pulverized to prepare a mixed cheonnyeoncho mixture.
The Cheonnyeoncho mixture prepared by the above method is configured to be homogenized by stirring at high speed in a mixing tank equipped with an impeller, and the content of water is adjusted so that the pulverized material of Cheonnyeoncho does not occur, so that the extraction efficiency of the active ingredient of Cheonnyeoncho is improved. You can increase it. In this case, it is preferable to use the water having a temperature of 35 to 60° C. so as to improve the extraction yield and not cause destruction of the active ingredient, and more preferably, water of 40 to 50° C. may be used.
In this step, 5 to 20 kg of raw materials of Cheonnyeoncho may be mixed with 70 to 90kg of water at 35 to 60°C to prepare the Cheonnyeoncho mixture.
In addition, in this step, after preparing the cheonnyeoncho mixture as described above, it is configured to further include the step of pressing the cheonnyeoncho mixture to induce damage or irritation to the plant cell walls of cheonnyeoncho to further increase the various active ingredients contained in the cheonnyeoncho. It can be extracted effectively. At this time, since the Cheonnyeoncho extract contains water as a solvent, the effect of improving the extraction efficiency by pressurization is higher by applying a uniform pressure to the tissue of Cheonnyeoncho.
Specifically, the high-pressure treatment as described above is an extraction technology that can be most efficiently utilized in the intake of nutrients and non-nutritional plant chemicals in the recent food field, and prevents the destruction of various active ingredients contained in the prepared cheonnyeoncho mixture. In order to be able to, it is possible to extract various active ingredients contained in Cheonnyeoncho in a short time by simultaneously applying the step of pressurizing at a pressure of 30 to 100 MPa in an environment in which gas flow is blocked from the outside and heating to a temperature of 40 to 60°C. It has the advantage of being able to easily obtain high-purity cheonnyeoncho components because there are few impurities.
When the pressurization is performed under a pressure of less than 30 MPa, there is a problem that the extraction rate of the active ingredient is not significantly improved due to a decrease in pressurization efficiency, and when the pressure exceeds 100 MPa, temperature control is difficult and there is a problem that the active ingredient may be destroyed. .
The step (b) is a step of preparing a Cheonnyeoncho extract by filtering the mixture of Cheonnyeoncho, in this step, filtering the Cheonnyeoncho mixture containing a large amount of the active ingredient of Cheonnyeoncho by the above extraction method with a filter membrane of 300 to 400 mesh Cheonnyeoncho extract can be prepared.
Cheonnyeoncho extract extracted by the above method contains a large amount of active ingredients such as polyphenols, flavonoids, dietary fiber, vitamin C, calcium, minerals, amino acids, etc. contained in Cheonnyeoncho, whitening, antioxidant, skin moisturizing, and wrinkle improvement upon introduction of the skin. It has an effect of improving skin beauty, such as, and even when ingested, it can exhibit significant effects such as prevention of adult diseases, anti-cancer and antioxidant.
However, the Cheonnyeoncho extract contains a large amount of pectin-based polymer polysaccharides exhibiting mucous properties.
Specifically, the polymer polysaccharides contained in the Cheonnyeoncho extract include reducing sugars such as glucose, arabinose, galactose, and xylose, and galacturonic acid, Pectin, a dietary fiber of polysaccharides such as arabinogalactan, rhamnose, etc., is included as a main component, whereby the cheonnyeoncho extract has rapid viscosity change and time-dependent non-inertial flow behavior (non-Newtonian flow behavior). That is, as the Cheonnyeoncho extract contains the polysaccharides as described above, when used in cosmetics or food production, when excessively mixed with water, the volume rapidly expands or exhibits excessive viscosity, resulting in poor palatability and feeling of use when used as cosmetics. Or there is a problem in that it is difficult to add an excessive amount of Cheonnyeoncho extract to food, and there is a problem that the effect due to the introduction of Cheonnyeoncho extract is somewhat inferior.
In particular, when using the Cheonnyeoncho extract in cosmetics or food manufacturing, when the concentration of the Cheonnyeoncho extract is added in excess of 5% (w/v) or more, the viscosity increases proportionally, and the solubility of the Cheonnyeoncho extract decreases As a result, the content of the Cheonnyeoncho extract is low, so that excellent physiologically active functions cannot be achieved.
Therefore, in the present invention, unlike the microbial fermentation step or acid treatment step used in the prior art, by irradiating the prepared Cheonnyeoncho extract with radiation to reduce the molecular weight of various high molecular polysaccharides contained in the Cheonnyeoncho extract, the viscosity of the Cheonnyeoncho extract can be drastically reduced. have.
Specifically, in the irradiation step as described above, when gamma rays (γ) are irradiated to the Cheonnyeoncho extract, water is decomposed in the Cheonnyeoncho extract to form free radicals, and the formed free radicals cause a chain reaction, and the change in the molecule of the polysaccharide is prevented. Induction, the polysaccharide becomes low molecular weight, and the viscosity of the extract of Cheonnyeoncho decreases. Cheonnyeoncho extract containing polysaccharides with reduced viscosity as described above can dramatically increase the amount of added when used in cosmetics or food manufacturing, and can support a large amount of various active ingredients contained in the Cheonnyeoncho extract, and eventually physiological activity The function can be further improved.
As an example, the cheonnyeoncho extract was irradiated with radiation having an intensity of 5 to 20 kilograms (kGy) for 3 to 24 hours, and the total absorption amount was adjusted to be 12 to 20 kilograms (kGy) to reduce polysaccharides and reduce molecular weight. Cheonnyeoncho extract containing a polysaccharide is filtered through an ultrafiltration membrane to contain a polysaccharide treated with low molecular weight having a molecular weight in the range of 05 to 5 kDa, so that the viscosity of Cheonnyeoncho extract can be obtained.
The cheonnyeoncho extract prepared as described above effectively decomposes pectin, a dietary fiber that is difficult to decompose by enzyme treatment, acid treatment, or heat treatment, and can minimize the destruction of active ingredients, so there is no denaturation of ingredients. In addition, it can be used stably by effectively removing various microorganisms.
Furthermore, in this step, in order to further improve the efficiency of the irradiation step, the catalyst is added at a concentration of 800 to 10,000 ppm to further improve the low molecular weight of polysaccharides, and thus the viscosity of the extract of Cheonnyeoncho can be further significantly reduced. .
The catalysts that serve as described above are representative examples of peroxide, peroxyacetic acid, titanium dioxide (TiO2), and the like, and preferably, when irradiated with radiation to the extract of Cheonnyeoncho, it is decomposed into water and oxygen, which is an eco-friendly peracetic acid. By using, the effect of reducing polysaccharides by irradiation can be further improved.
As described above, the extract of Cheonnyeoncho, which has undergone the irradiation step, has a low molecular weight of pectin-based polysaccharides, which can significantly improve the absorption of polysaccharides caused by skin introduction or ingestion, while lowering the viscosity so that it is excellent even when an excessive amount of Cheonnyeoncho extract is added in the manufacture of cosmetics or food. It is possible to manufacture Cheonnyeoncho cosmetics or foods having palatability and physical properties.
According to the method for preparing the Cheonnyeoncho extract according to the present invention as described above, by using a combination step of low-temperature and high pressure extraction and irradiation, the active ingredient contained in Cheonnyeoncho is effectively extracted, and the polysaccharide contained in Cheonnyeoncho is reduced in molecular weight to reduce viscosity. According to this method, it is possible to prepare a Cheonnyeoncho extract with excellent physical properties that can improve physiological activity by increasing the content of Cheonnyeoncho when manufacturing cosmetics or foods.
The Cheonnyeoncho extract prepared by the above method has a low viscosity and is easy to disperse even when an excess of 10% or more is added, and since it contains a low-molecularized polysaccharide, it has excellent physiological activity and can be effectively used in the manufacture of cosmetics or foods.
In addition, in order to improve the dispersion of the Cheonnyeoncho extract and further increase the content thereof, a solvent such as 1,2-hexanediol, butylene glycol, ethylhexanediol, etc. may be additionally included, and 1 to 5 kg of the solvent By mixing, the physical properties of the Cheonnyeoncho extract can be adjusted.
In addition, the Cheonnyeoncho extract is configured to additionally contain additives consisting of vitamins, oils, surfactants, whitening agents, soothing agents, chelating agents, coloring agents, fatty acids, antioxidants, flavoring agents, preservatives, neutralizing agents, or mixtures thereof to suit various purposes. It can be used in the manufacture of suitable cosmetics, and the Cheonnyeoncho extract can be prepared in various forms such as serum, lotion, oil, cream, essence, and the like.
Hereinafter, the present invention will be described in more detail by way of examples.
The examples presented are only specific examples of the present invention, and are not intended to limit the scope of the present invention.
<Example 1>
Cheonnyeoncho extract was prepared using the method as shown in FIG.
First, the raw materials were washed with water using the stems and fruits of Cheonnyeoncho as raw materials, and then the washed raw materials were put into a thorn removal device, and the thorns were removed for 40 minutes. In order to remove thorns from the raw material, a brush is fixedly formed, and a thorn removal device capable of forming a vacuum in the interior receiving space was used.
The raw material from which the thorns were removed was washed with water in the same way as described above, and then passed through a drying device equipped with a conveyor belt, a circular tunnel-shaped housing, and an air injection unit to remove moisture, and the moisture-removed raw material was supplied to a grinder.
Water at 40°C was supplied to the mill to which the raw materials of Cheonnyeoncho were supplied, and the raw materials of Cheonnyeoncho were crushed for 15 minutes to prepare a mixture of Cheonnyeoncho, and after supplying the prepared Cheonnyeoncho mixture to a mixing tank, a homogeneous mixture was prepared by continuously stirring. When preparing the mixture, the mixture was heated to a temperature of 40 to 50° C., and the viscosity was checked, and water was additionally supplied to prevent aggregation. For the pulverization, a pulverizer equipped with a motor capable of high-speed rotation of 2 horsepower and a double-edged knife was used, and a mixing bath provided with two impellers was used to prepare a mixture.
After supplying the cheonnyeoncho mixture prepared by the above method to a high-pressure homogenizer (coldisostatic press), high-pressure treatment was performed at a temperature of 50°C and a pressure of 80 MPa, and the high-pressure treated cheonnyeoncho mixture was filtered through a 300 mesh filter network. Sieve (sieving) to obtain a Cheonnyeoncho extract.
After charging the obtained Cheonnyeoncho extract into a Co-60 gamma ray irradiation system (point source AEC, IR-79), a 25 MeV transformer type electron accelerator (ELV-8Model, EB) to obtain a total absorbed dose of 10 kGy at a dose rate of 10 kGy per hour. Tech) was used to irradiate an electron beam with a beam current of 145mA, and the absorbed dose of the irradiation was operated at 10kGy to make the polysaccharide low in molecular weight, and the low-molecularized Cheonnyeoncho extract was subjected to ultrafiltration with a 150 mesh filtration membrane. Was obtained. The obtained Cheonnyeoncho extract was stored in a refrigerator at -20°C to prevent deterioration.
<Example 2>
After adding peracetic acid to the prepared Cheonnyeoncho extract at a concentration of 3,000 ppm, a Cheonnyeoncho extract was prepared in the same manner as in Example 1 except for irradiation with radiation.
<Comparative Example 1>
The mixture of Cheonnyeoncho before high-pressure treatment of Example 1 was filtered through a 300 mesh filter to obtain a Cheonnyeoncho extract.
<Comparative Example 2>
The mixture of Cheonnyeoncho, subjected to high pressure treatment in the same manner as in Example 1, was filtered through a 300 mesh filter network to obtain a Cheonnyeoncho extract.
<Experimental Example 1> Analysis of ingredients of active ingredients
In order to analyze the extraction efficiency of the active ingredient contained in the prepared Cheonnyeoncho extract, component analysis of the polyphenols and flavonoids contained in the Cheonnyeoncho extract prepared by the method according to Example 1 and Comparative Example 1 was performed, and the results are shown below. It is shown in Table 1. The component analysis was measured by performing the Maksimovic method, and the polyphenol component analysis was evaluated by measuring the absorbance with light at a wavelength of 725 nm, using garlic acid (Sigma Aldrich) as a standard material. The flavonoid component analysis was evaluated by adding aluminum chloride hexachloride to the Cheonnyeoncho extract and measuring the absorbance with light having a wavelength of 430 nm.
Polyphenol (mg/g) Flavonoids (mg/g) Example 1 479 ± 019 123 ± 007 Comparison Table 1 336 ± 021 094 ± 011
As shown in Table 1, compared to the contents of polyphenols and flavonoids, the Cheonnyeoncho extract prepared by the method according to Comparative Example 1 contained flavonoids and polyphenols contained in the Cheonnyeoncho extract of Example 1 prepared by the high pressure extraction method. It was confirmed to be higher, and through this, it could be confirmed that the extraction efficiency was significantly improved by high pressure extraction. <Experimental Example 2> Viscosity Analysis and Molecular Weight Analysis In order to analyze the viscosity of the prepared Cheonnyeoncho extract, Example 1, The yield strength of the Cheonnyeoncho extract prepared by the method according to Example 2 and Comparative Examples 1 and 2 was analyzed, and the results are shown in Table 2 below. Yield analysis was evaluated by measuring the shear stress while changing the shear rate from 0 to 300s-1 for the water extract of Cheonnyeoncho according to the concentration at 20°C using a viscosity analyzer.
Yield force (Pa) Example 1 1639 ± 047 Example 2 1327 ± 036 Comparative Example 1 21,09 ± 049 Comparative Example 2 2267 ± 053
As shown in Table 2, all of the prepared Cheonnyeoncho extracts showed a condition of 0 <n <1 with a fluidity index (n), and it was confirmed that they exhibit a non-inertial flow behavior that is not governed by Newton's law, in particular Comparative Example 2 In the case of the Cheonnyeoncho extract of Comparative Example 1, it was confirmed that the yield power was the highest compared to the Cheonnyeoncho extract of Comparative Example 1, and it was confirmed that the viscosity was the highest. On the other hand, the Cheonnyeoncho extract of Example 2 yielded even compared to Example 1 It was confirmed that the strength was low, and it was confirmed that the viscosity was significantly reduced through such a reduction in yield strength, and it was confirmed that the viscosity was significantly lowered compared to Comparative Examples 1 and 2. Through the results as described above, radiation Through the irradiation, it was confirmed that the viscosity decreased due to the promotion of low molecular weight, and it was confirmed that the addition of the catalyst can further promote the low molecular weight. In addition, according to Examples 1 and 2 and Comparative Examples 1 and 2 Cheonnyeoncho powder was prepared by lyophilizing the Cheonnyeoncho extract prepared by the method, and 10 to 30g of the prepared powder was mixed with 100 mL of water at 20°C, and then vigorously stirred for 5 minutes to check the solubility, Comparative Example 1 In the case of manufacturing by the method according to, aggregation was confirmed from the case of adding 10g of Cheonnyeoncho powder, and when prepared by the method according to Comparative Example 2, aggregation was confirmed from the case of adding 8g of Cheonnyeoncho powder. Through the results as described above, it was confirmed that the viscosity of the polymer was reduced significantly due to the low molecular weight through the irradiation treatment in the method according to Example 1, and the solubility of the Cheonnyeoncho extract prepared by the method according to Example 2 was markedly It could be confirmed that it increased.
Through the results as described above, the Cheonnyeoncho extract prepared by the method for preparing the Cheonnyeoncho extract according to the present invention contains the active ingredient contained in Cheonnyeoncho at a higher concentration due to the complex step of low temperature and high pressure extraction and irradiation, It was confirmed that polysaccharides contained in Cheonnyeoncho have low molecular weight and thus can be effectively used in the manufacture of cosmetics or foods because of their low viscosity.
2. Nano-emulsion cosmetic composition manufacturing
1. Step 1
a) Dissolve 10 to 20 kg of glyceline and 0.1 to 3 kg of hydrogenate lecithin at 75°C using a homomixer at 200 rpm for 10 minutes
*b) Completely dissolved 20 to 30 kg of purified water, 0.1 to 1 kg of Cheonnyeoncho extract, 0.1 kg to 50 kg of ethanol, and 10 to 20 kg of dipropylene glycol at room temperature
c) Caprylic/Capric triglyceride 20 to 30 kg neopentyl glycol dicaprate 0.5 to lkg, stearic acid 0.5 to 2% kg, C12-20 alkyl glucoside 0.1 to 1 kg, C14 -22 Alcohol 0.1 to 1kg dissolved at 75℃ using a homomixer at 1500rpm for 5 minutes
2. Second stage
The extract prepared in the first step is passed through 1 to 3 times using a High Prossure Homogenizer device to produce 150 to 200 nanometers.
3. Step 3
Mixing 1,2-hexanediol 1 to 2k heated to 50° C. to the mixture prepared through the second step
4. 4th fixed
Prepare a nanoemulsion cosmetic composition by mixing the preparations a and c prepared in the first step and the mixture prepared in the third step
3. Nado emulsion cosmetic composition particle size analysis report
1. Experiment method
The sample was diluted 1000 to 2000 times with distilled water and proceeded. Particle analysis was performed using Malvern Zetasizer Nano S90.
2. Experiment result
The experimental results are as follows. Cosmetic A showed similar particle sizes in all of A-1, A-2, and A-3, and the particle size was measured to be about 260nm. In the case of cosmetic B, the particle size of B-3 was 141.2 nm, showing the smallest size. In addition, it was confirmed that the PdI value was 0.055 and the dispersion degree was low, so that the particle size was uniformly prepared.
1) A-1

2) A-2

3) A-3

4) B-1

5) B-2

6) B-3

After basic makeup (skin, lotion, cream, essence, eye cream, etc.), the cosmetic composition of the present invention removes about 01g-02g of the contents of the eyes and mouth to the areas with traces of acne, After applying firmly as if filling, finish as if tapping with your fingers, wait for it to dry completely, and apply color makeup as needed. In this case, if the uneven areas of the skin are slightly deep, it is desirable to apply a thin layer of 2-3 times in a row at 1-minute intervals, dry completely, and apply color makeup if necessary.
IV. Raw material stirring method 2
On the other hand, the raw material stirring method based on the above-described content comprises the steps of preparing raw materials; It may include the step of obtaining a composition containing the by-product extract of cheonnyeoncho extract molecule based on the stirred raw material. In addition, the raw material stirring method based on the above-described contents includes the step of configuring to homogenize the mixture of cheonnyeoncho by stirring using a stirring device, and can prepare a cheonnyeoncho extract.
Hereinafter, a stirring device for performing stirring will be described among the above-described constitutive features. 4 to 6, the stirring device is fixedly installed on the stand 80, and the tank 10 with the outlet 12 formed at the bottom, and the inside of the tank 10 can be lifted up and down and rotated at the same time. It consists of a rotating rod 20 provided so as to, a driving motor 30 for driving the rotating rod 20, and an impeller 40 formed at the lower end of the rotating rod 20 to agitate the contents. The tank 10 is opened at the top and the bottom is concave in an elliptical shape around the central outlet 12. The tank 10 is preferably made of stainless steel to prevent corrosion. The object (eg, mixed liquid, etc.) that has been mixed in the tank 10 is discharged to the outside through the discharge port 12. The rotating rod 20 is provided so as to be able to lift the inside of the tank 10 in an upward direction. Accordingly, a lifting guide 82 is installed on one side of the rotating rod 20 to guide the rotating rod 20. A driving motor 30 for rotating the rotating rod 20 is provided above the rotating rod 20. The rotating rod 20 is provided with an upper rotating rod 22 connected to a driving motor and a lower rotating rod 24 on which the impeller 40 is installed. Here, the upper rotating rod 22 and the lower rotating rod 24 are connected by a shaft joint 26 to facilitate exchange of the impeller 40. The driving motor 30 is provided on the top of the rotating rod 20 and generates a rotational force by applying power. The drive motor 30 is provided with a reducer 32 in order to reduce the rotational speed and improve the torque between the rotating rod 20. The impeller 40 is formed at the lower end of the rotating rod 20, and the blade surface 42 of the impeller 40 is provided to be inclined downward in the rotational direction of the rotating rod 20 so that the object flows downward by the rotation of the impeller 40. It is desirable to be. Here, the blade surface 42 of the impeller 40 is more preferably provided to be inclined downward at 45°. In addition, it is preferable that a separate guide 44 is formed at the end of the impeller 40 in a tangential direction to the rotational direction around the rotating rod 20 so that the flow of the object does not deviate in the radial direction.
7 to 9 are views schematically showing a stirring device according to another embodiment of the present invention. Hereinafter, a description will be made based on the above-described contents, but focusing on a portion having constitutive characteristics. 7 to 9, the stirring device is fixedly installed on a base panel 90 provided on a lower surface of a plurality of support rods, and a stand 80 provided on the base panel 90, and an outlet at the bottom. A tank 10 is formed, a rotating rod 20 provided to be able to move up and down along the inside of the tank 10 and rotate at the same time, and a drive motor 30 for driving the rotating rod 20 , An impeller 40 formed at the lower end of the rotating rod 20 to stir the contents, and a mounting unit 100 mounted on the base panel 90.
Here, the mounting unit 100 includes a lower body 110 under the base panel 90 and a first central body 111 provided on an upper portion of the lower body 110 Wow. It is provided so as to flow back and forth in one direction on the first central body 111, the first support bar 1001 of the support bars is mounted vertically, and the installed first support bar 1001 is pulled or pushed in the horizontal direction. The 1-1 front-rear fluid body 112 for fixing, and the first central body 111 are provided so as to flow back and forth in the other direction, and the second support bar 1002 of the support bars is mounted vertically through the top and bottom, and the mounted A 1-2 front-rear fluid (113) for pressing or pressing the second support rod (1002) in a horizontal direction, and a second central body (120) provided on the upper portion of the first central body (111). The second central body 120 is driven forward and backward in a sliding manner based on a cylinder module in one direction, and the first support rod 1001 is mounted vertically, and the mounted first support rod 1001 is mounted in a horizontal direction. The 2-1 front-rear fluid 121 for pulling or pushing and pressing and fixing, and the second central body 120 are driven forward and backward in a sliding manner based on a cylinder module, and the second support rod 1002 is vertically It is mounted through, and is located at the upper end of the 2-2 front and rear fluid 122 and the 2-1 front and rear fluid 121 for pulling or pushing the mounted second support rod 1002 in the horizontal direction The second central body 120 is driven forward and backward in a sliding manner based on a cylinder module in one direction, and the first support rod 1001 is mounted vertically, and the mounted first support rod 1001 is horizontally It is located on the upper end of the 3-1 front-rear fluid 123 and the 2-2 front-rear fluid 122 for pulling or pushing in the direction, and the cylinder module-based on the other side of the second central body 120 It is driven in the front-rear direction in a sliding manner, and the second support rod 1002 is mounted vertically, and the 3-2 front-rear fluid for pressing or pushing the mounted second support rod 1002 in a horizontal direction ( 124).
Here, the first support rod 1001 is a 1-1 stage mode in which a pulling force is applied inward by the 1-1 first front-rear fluid 112, and the 2-second stage 2 in the 1-1 stage mode. 1 In the second-stage mode in which the inward pulling force is secondly applied by the front-rear fluid 121 and the inward pulling force by the 3-1 front-rear fluid 123 in the second-stage mode It may be fixed based on the 3-1 stage mode applied in the third order.
The second support rod 1002 includes a 1-2 stage mode in which a pulling force is applied inward due to the backward movement of the 1-2nd front-rear fluid 113, and the 2-second stage in the 1-2 stage mode. 2 In the second-stage mode in which the pulling force is applied to the inside by reversing the front and rear fluid 122, and in the second-stage mode by the backward movement of the 3-2 forward-rear fluid 124 The pulling force can be fixed based on the 3-2 stage mode applied in the third order.
In addition, the mounting unit 100, the 1-1 up and down flow body 125 that is moved up and down based on the cylinder module in the lower body 110, and the 1-1 up and down fluid in the A 1-1 extension body 126 provided on the side to be in contact with the upper surface of the 1-1 front and rear fluid 112 and one end of the 2-1 front and rear fluid 121, and a cylinder module in the lower body 110 The 1-2 vertical fluid 127 that moves up or down based on, and the upper surface of the 1-2 front and rear fluid 113 in the 1-2 vertical fluid 127 and the 2-2 front and rear fluid It may include a 1-2 extension body 128 provided to the side so as to contact one end of (122).
Here, based on the descending of the 1-1th up and down fluid 125, the 1-1th extension 126 depresses the 1-1st front and rear fluid 112, and the 1-2nd up and down fluid 127 ), the 1-2 extension body 128 may press down the 1-2 forward/rear fluid 113. The mounting unit 100 includes a 2-1 up and down fluid 131 that is located outside the 1-1 up and down fluid 125 in the lower body 110 and is moved up or down, and the 2- 1 In the vertical fluid 131, the top surface of the 1-1 extension body 126, the top surface of the 1-1 extension body 126, and the side surface of the 3-1 front and rear fluid body 123 A 2-1 extension body 132, a 2-2 up and down fluid body 133 located outside the 1-2 up and down fluid 127 in the lower body 110 and flowing up or down, and the second From the 2-2 vertical fluid 133 to the top surface of the 1-2 extension body 128, the top surface of the 1-2 extension body 128, and the end of the 3-2 front-rear fluid body 124 It may include a 2-2 extension body 134 provided.
Based on the descending of the 2-1 vertical fluid 131, the 2-1 extension body 132 lowers the top surface of the 1-1 extension body 126 and the top surface of the 1-1 extension body 126. Pressing, and based on the descending of the 2-2 vertical fluid 133, the 2-2 extension body 134 is the upper surface of the 1-2 extension body 128 and the upper surface of the 1-2 extension body 128 Pressing down, the 1-1 extension body 126 and the 1-2 extension body 128 is a motor built-in on the 1-1 up and down fluid body 125 and the 1-2 up and down fluid body 127 Based on each vertical axis rotation drive, the 2-1 extension body 132 and the 2-2 extension body 134 is the 2-1 vertical fluid 131 and the 2-2 vertical fluid ( 133) on the basis of the built-in motor, each vertical axis rotation drive. These vertically axially rotated components are provided in a quadrangular shape, and can generate a fixing force that presses and presses adjacent components based on the axial rotation.
The stirring device is provided with a plurality of the first stirring device (1) and the second stirring device (2), the lower body 110 of the first stirring device (1) and the second stirring device (2). 2 Between the upper body 110, a first connection module 210 for connecting the lower body 110 and the second lower body 110 is provided, and a second connection module outside the lower body 110 220 wherein the lower portion of the outer side, the third connection module (230) are provided, the first agitator (1) and the second stirrer (2) of the second lower body 110 is provided A base driving body 240 for driving the first connection module 210 to the third connection module 230 by pulling or pushing between the left and right in a sliding manner based on a cylinder module, an actuator, etc. is provided.
The first connection module 210 includes a first column 211 provided to be vertically movable in the base driving body 240, and a first driving region provided at an upper end of the first column 211 A portion 212, a 1-1 fitting portion 213 coupled to one end of the lower body 110 at one side of the first driving area portion 212 by an extra fitting method, and the first driving area portion On the other side of the second lower body 110, a 1-2 second fitting portion 214 is provided that is coupled to one end portion of the second lower body 110 by an extra fitting method. The second connection module 220 includes a second column body 221 provided to be vertically movable in the base driving body 240 and a second driving region provided at an upper end of the second column body 221 A portion 222 and a second fitting portion 223 are provided at one side of the second driving area portion 222 to engage the other end of the lower body 110 by an extra fitting method.
The third connection module 230 includes a third pillar body 231 provided to be vertically flowable from the base driving body 240 and a third driving region provided at an upper end of the third pillar body 231 A portion 232 and a third fitting portion 233 coupled to the other end of the lower body 110 by an extra fitting method at one side of the third driving area portion 232 are provided, and the first-1 The fitting portion 213 and the second fitting portion 223 are rotated in opposite directions to each other to generate a rotation fixing force for the lower body 110, and the 1-2 fitting portion 214 and the second fitting portion 214 3 The fitting portions 233 are rotated in opposite directions to each other to generate a rotation fixing force for the second lower body 110, and the first to third pillars 211 to 231 It is provided to enable reciprocating flow in a sliding manner between the left and right in the base driving body 240.
As described above, the present invention is not limited to the described embodiments, and it is apparent to those of ordinary skill in the art that various modifications and variations can be made without departing from the spirit and scope of the present invention. Therefore, it should be said that such modifications or variations belong to the claims of the present invention. On the other hand, the above-described driving method according to the forward and backward flow or up and down movement is implemented to be driven based on a cylinder module, an actuator, and other applicable known flow means, and does not require advanced technology of a new operation method. It is obvious to a person skilled in the art

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Claims (2)

In the raw material stirring method,
Purifying for 24 hours by mixing water and ethanol at room temperature to extract the extract of bokbunja by-product; And including the step of stirring so that the mixture is evenly mixed through a stirring device,
The stirring device,
A base panel 90 provided on a lower surface of a plurality of support rods,
A tank 10 fixedly installed on the stand 80 provided on the base panel 90 and having an outlet at the bottom thereof,
A rotating rod 20 provided to be able to move up and down along the inside of the tank 10 and rotate at the same time,
A driving motor 30 for driving the rotating rod 20, an impeller 40 formed at the lower end of the rotating rod 20 to agitate the contents,
It includes a mounting unit 100 mounted on the base panel 90,
The mounting unit 100,
A lower body 110 under the base panel 90,
A first central body 111 provided on an upper portion of the lower body 110 and.
It is provided so as to flow back and forth on the first central body 111 in one direction, and the first support bar 1001 of the support bars is mounted vertically, and the mounted first support bar 1001 is pulled or pushed in a horizontal direction. 1-1 front and rear fluid for fixing (112) and,
It is provided so as to flow back and forth on the first central body 111 in the other direction, and the second support bar 1002 of the support bars is mounted vertically, and the installed second support bar 1002 is pulled or pushed in the horizontal direction. A 1-2 front and rear fluid 113 for fixing ,
A second central body 120 provided on an upper portion of the first central body 111 and.
The second central body 120 is driven forward and backward in a sliding manner based on a cylinder module in one direction, and the first support rod 1001 is mounted vertically, and the mounted first support rod 1001 is mounted in a horizontal direction. A 2-1 front and rear fluid 121 for pressing or pressing and fixing by pulling or pushing ,
The second support rod 1002 is driven forward and backward in a sliding manner based on a cylinder module on the other side of the second central body 120, and the second support rod 1002 is mounted vertically, and the mounted second support rod 1002 is mounted horizontally. A 2-2 front and rear fluid 122 for pressing or pressing and fixing by pulling or pushing ,
It is located at the upper end of the 2-1 front-rear fluid 121, is driven forward and backward in a sliding manner based on a cylinder module on the second central body 120, and the first support rod 1001 penetrates up and down. A 3-1 front and rear fluid 123 for pressing or pressing the mounted first support rod 1001 in a horizontal direction ,
It is located at the upper end of the 2-2 front-rear fluid 122, is driven in the front- rear direction in a sliding manner based on a cylinder module on the second central body 120 to the other side, and the second support rod 1002 penetrates up and down. It is mounted, and includes a 3-2 front and rear fluid 124 for pressing or pressing the mounted second support rod 1002 in a horizontal direction ,
The first support bar 1001,
The 1-1 stage mode in which a pulling force is applied inward by the 1-1 front and rear fluid 112, and the 2-1 front and rear flow body 121 pulls inward in the 1-1 stage mode In the 2-1 stage mode in which the force is applied secondarily, and in the 3-1 stage mode in which the force pulled inward by the 3-1 front and rear fluid 123 in the 2-1 stage mode is applied in the third stage. Is fixed based on
The second support rod 1002,
In the 1-2nd stage mode in which a pulling force is applied inward by the retraction of the 1-2nd front-rear fluid 113, and the 2-2nd front-rear fluid 122 in the 1-2nd stage mode A second-stage mode in which a pulling force is applied to the inside by the second stage, and a third inward pulling force is applied thirdly by the backward movement of the 3-2 front and rear fluid 124 in the second-stage mode. Fixed based on 3-2 step mode,
The mounting unit 100,
In the lower body 110, the first-first vertical fluid 125 is moved up or down based on the cylinder module, and the first-first front-rear fluid 112 in the first-first vertical fluid 125 A 1-1 extension body 126 provided on the side to be in contact with the top surface and one end of the 2-1 front and rear fluid 121,
From the lower body 110 to the 1-2 up and down fluid 127 that moves up or down based on the cylinder module , and the 1-2 up and down fluid 127 to the 1-2 front and rear fluids 113 It further includes a 1-2 extension body 128 provided on the side to be in contact with the upper surface and one end of the 2-2 front and rear fluid 122,
Based on the descending of the first-first vertical fluid 125, the first-first extension 126 pushes down the first-first front and rear fluid 112,
Based on the descending of the 1-2 vertical fluid 127, the 1-2 extension body 128 pushes down the 1-2 front and rear fluid 113,
The mounting unit 100,
A 2-1 up and down fluid 131 located outside the 1-1 up and down fluid 125 in the lower body 110 and flowing up or down,
In the 2-1 vertical fluid 131, the top surface of the 1-1 extension body 126, the top surface of the 1-1 extension body 126, and the ends of the 3-1 front-rear fluid body 123 are in contact with each other. The 2-1 extension body 132 provided as a side,
A 2-2 up and down fluid 133 located outside the 1-2 up and down fluid 127 in the lower body 110 and flowing up or down,
In the 2-2 vertical fluid 133, the top surface of the 1-2 extension body 128, the top surface of the 1-2 extension body 128, and the end of the 3-2 front-rear fluid body 124 are in contact with each other. Raw material stirring method further comprising a 2-2 extension body 134 provided to the side.
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