KR20180072456A - Method for preparing extracts of Eastern prickly pear and cosmetic compositions and food composition comprising extracts of Eastern prickly pear prepared thereby - Google Patents

Abstract

The present invention provides a method for preparing an extract of Eastern prickly pear, and a functional cosmetic composition and a food composition comprising the same prepared thereby. According to the method according to the present invention, by using a composite process of low temperature and high pressure extraction and radiation irradiation, effective ingredients included in Eastern prickly pear are effectively extracted, and polysaccharide included in Eastern prickly pear is made to low molecules to reduce viscosity. Thus, it is possible to increase the content of Eastern prickly pear when producing cosmetics or food, and it is possible to prepare an extract of Eastern prickly pear having an excellent biological activity. The extract of Eastern prickly pear prepared by the method has a low viscosity, thereby being easily dispersed even when an excessive amount of 5% or more is added, and has low molecular weight polysaccharide, thereby being effectively used for preparing cosmetics or food by having an excellent biological activity.

Description

FIELD OF THE INVENTION The present invention relates to a method for preparing an extract of Chenille seeds, and a cosmetic composition and a food composition comprising the Chenille seed extract prepared by the method and a composition for extracting an extract from Eastern prickly pears,

The present invention relates to a method for producing a tsunami extract, a functional cosmetic composition comprising the tsunami extract, and a food composition.

Cacti are highly adaptable to dry climates and have long been used as a source of carbohydrates and vitamins, particularly in the deserts of many countries suffering from food shortages and food shortages. In the Orient, constipation, diuretic activity, For the purpose of treatment such as promotion, skin disease, rheumatism or burn, cactus fruit and stem were used.

In recent years, the value of cactus has been recognized and cultivation is encouraged, and processed foods such as jam, jelly, and juice using cactus have been actively developed.

Among the above-mentioned cacti, Eastern prickly pear ( Opuntia humifusa ) has a characteristic of maintaining its strong vitality without freezing by reducing its own moisture to less than half even at a temperature of minus 20 ° C or below. Polyphenols, flavonoids, Dietary fiber, vitamin C, calcium, minerals, amino acids, complex polysaccharides, and the like. Various studies have been carried out to utilize the millennium ginseng, as it is known that the millennium ginseng has various physiological activities.

For example, Korean Patent Laid-Open No. 10-2011-0117514 discloses a technical content of a natural emulsifier prepared by mixing a citrus extract and a vegetable oil.

However, the emulsifier prepared by the method disclosed in the above-mentioned document 1 has a problem that it is difficult to add a large amount of the citrus jujube extract due to the viscous material contained in large quantities in the millennia early stage, and the binding strength between the citrus extract and the vegetable oil is weak and the long-term stability is poor.

As another example, Korean Patent Laid-Open No. 10-2011-0130820 discloses a method for preparing a fermented cucumber extract, which comprises mixing a herbal cosmetics and a nitrogen source in a cucumber seedling, fermenting the microorganism after the fermentation, And a method for performing the method is disclosed.

However, the herbal cosmetics manufactured by the method disclosed in Document 2 have a long manufacturing process and cause problems such as contamination by microorganisms.

Therefore, there is a need for research on a method that can overcome such drawbacks.

Korean Patent Laid-Open No. 10-2011-0117514 (published on October 27, 2011) Korean Patent Laid-Open No. 10-2011-0130820 (Publication date: 2011.12.06) Korean Patent Publication No. 10-2016-0092287 (Publication date: 2016.08.04)

The present invention has been conceived to solve the problems of the prior art as described above, and it is an object of the present invention to provide a method of extracting various active ingredients contained in the early millennia by a high yield, And to provide a cosmetic composition and a food composition using the same.

In order to accomplish the above object, the present invention provides a method for producing a mixture of (a) preparing a mixture of a tillering starch raw material containing a tillering root and a tillering starch with water at 35 to 60 ° C and homogenizing the mixture; And (b) filtering the mixture of the tsunami seeds to produce a tsunami extract.

In addition, the step (a) may further comprise the step of pressurizing the mixture at a pressure of 30 to 100 MPa after preparing the mixture.

In addition, the step (b) may further include the step of irradiating the tubercanthopteran extract with radiation after preparing the tubercanchotissue extract.

In addition, the present invention provides a cosmetic composition comprising 5 to 20 parts by weight of a quinternial extract prepared by the above-described method, 70 to 90 parts by weight of water and 1 to 10 parts by weight of a moisturizer.

In addition, the present invention provides a ternary seedless food composition comprising 5 to 20 parts by weight of a tsunami extract prepared by the above-described method and 75 to 90 parts by weight of water.

According to the present invention, it is possible to effectively extract active ingredients contained in the early millennium and to lower the viscosity of the polysaccharide contained in the millennium by using a hybrid process of low temperature high pressure extraction and irradiation. Accordingly, it is possible to manufacture a tsunami extract having an excellent physical property that can improve the physiological activity by increasing the content of tsunami seeds in the production of cosmetics or foods.

The Chennaiensis extract prepared by the above method has a low viscosity and is easy to be dispersed even when added in an amount exceeding 5%, and can be effectively used for cosmetics or foods because of its excellent physiological activity including low molecular weight polysaccharides.

FIG. 1 is a process drawing showing a method of producing a perennial plant extract according to the method of Example 1. FIG.

Hereinafter, the present invention will be described in detail.

The present invention relates to a method for preparing a mixture of (a) a step of mixing a raw material of a perennial plant including a perennial plant stem and a perennial plant with water at 35 to 60 ° C and then homogenizing the plant to prepare a perennial plant mixture; And (b) filtering the mixture of the tsunami seeds to produce a tsunami extract.

The step (a) is a step of mixing the raw material of the millennia including the root of the perennial plant and the fruit of the perennial plant with water at 35 to 60 ° C and then homogenizing the mixture to prepare a mixture of the perennial plant. In this step, After mixing with the raw material, the mixture can be homogenized using a homogenization treatment apparatus to prepare a mixture of the two materials.

The preparation of the mixture may be carried out using a conventional food shredding apparatus, preferably using a crushing apparatus equipped with a strong motor having a power of 2 hp or more and a double-edged sword, To 300-400 mesh average particle size and homogenously milled in millennia due to mixing with heated water to produce a mixed ternary starter mixture.

The mixture prepared by the above method was stirred at a high speed in a mixing tank equipped with an impeller to homogenize the mixture, and the water content was adjusted so as not to cause agglomeration of the milled product, . At this time, it is preferable to use water having a temperature of 35 to 60 DEG C, more preferably 40 to 50 DEG C, so as to improve the extraction yield and not cause destruction of the active ingredient.

In this step, the mixture of water and the water is mixed at a weight ratio of 1:20 to 1: 5 to prepare the mixture.

In addition, the present step further comprises a step of preparing the above-mentioned mixture of the above-mentioned ternary premixes, followed by the step of pressurizing the mixture of the above-mentioned ternary premixes to induce damage or stimulation to the plant cell walls of the ternary- It can be extracted effectively. At this time, since the water extract is contained as a solvent, the extraction efficiency by pressurization is higher by applying uniform pressure to the tissue of the millennium.

Specifically, the high-pressure treatment as described above is an extraction technique that can be most effectively utilized in the intake of nutrients and non-nutrient phytochemicals in the field of food, and it can prevent destruction of various active ingredients contained in the manufactured water- It is possible to extract various active ingredients contained in the millennia early in a short period of time by simultaneously applying a process of pressurizing at a pressure of 30 to 100 MPa and heating at a temperature of 40 to 60 ° C in an environment in which the outside and gas circulation are blocked And has an advantage of being able to easily obtain a high-purity ternary-plant component since there are few impurities.

When the pressure is lower than 30 MPa, there is a problem that the pressure efficiency is lowered and the extraction rate of the effective component is not greatly improved. When the pressure exceeds 100 MPa, the temperature control becomes difficult and the effective component may be destroyed .

The step (b) is a step of filtering the mixture of the perennial plant and producing the perennial plant extract. In this step, the perennial plant containing a large amount of the active ingredient of the perennial plant is filtered through a 300-400 mesh filtration membrane Can be prepared.

The extract of Chennai Seed extract obtained by the above method contains a large amount of active ingredients such as polyphenols, flavonoids, dietary fibers, vitamin C, calcium, minerals and amino acids contained in the perennial plant, and provides whitening, antioxidation, skin moisturizing, Etc., and can have significant effects on prevention of adult diseases, anticancer and antioxidation even when ingested.

However, the citrus extracts contain a large amount of pectin-based high-molecular polysaccharide having mucilage.

Specifically, the polysaccharide contained in the citrus extract may contain reducing sugars such as glucose, arabinose, galactose, and xylose, and may be galacturonic acid, The present invention includes the pectin, which is a dietary fiber of polysaccharides such as arabinogalactan, rhamnose, etc. as a main ingredient, whereby the above-mentioned Tianyucho extract has rapid viscosity change and time-dependence of non-Newtonian flow behavior flow behavior. That is, when the polysaccharide is contained in polysaccharides as described above, the polysaccharide is excessively mixed with water when it is used in the production of cosmetics or foods, the volume rapidly expands or the polysaccharide exhibits excessive viscosity, Or an excessive amount of quinine-annual herb extract is added to food, there is a problem that the effect due to the introduction of quinine-annual herb extract is somewhat deteriorated.

Particularly, when the extract is used in cosmetics or foods, the viscosity of the extract is increased by 5% (w / v) or more, and the solubility of the extract is decreased, The content thereof is low, so that it is impossible to achieve a superior physiological activity function.

Therefore, in the present invention, by irradiating the radiopharmaceuticals produced by the microorganism fermentation process or the acid treatment process, which are different from the conventional microbiological fermentation process or the acid treatment process, the various high-molecular polysaccharides contained in the tsunami seed extract can be made low molecular weight, have.

Specifically, when the irradiation of the gamma ray (?) Is applied to the extract of Ganoderma lucidum, the free radicals are formed by the decomposition of water in the Ganoderma lucidum extract, the free radicals formed result in a chain reaction, And the polysaccharide becomes low molecular weight, so that the viscosity of the perilla seed extract decreases. As described above, the tsunami extract containing the polysaccharide having reduced viscosity can significantly increase the addition amount when used in the production of cosmetics or foods, and it is possible to carry a large amount of various active ingredients contained in the tsunami extract, The function can be further improved.

For example, irradiation of radiation of 5 to 20 kilograms (kGy) intensity for 1 to 20 hours to the above-described T. acutissimum extract is performed so that the total absorption amount becomes 10 to 20 kilograms (kGy) to lower the polysaccharide to low molecular weight, The extract of the perennial herb including polysaccharides is filtered with an ultrafiltration membrane to include a low molecular weight polysaccharide having a molecular weight ranging from 0.5 to 5 kDa to obtain a perennial herb extract having a remarkably reduced viscosity.

The perennial plant extract thus prepared effectively decomposes pectin, which is a dietary fiber which is difficult to be decomposed by enzymatic treatment, acid treatment or heat treatment, which is conventionally known, and can minimize the destruction of the active ingredient, However, it can effectively remove various microorganisms and use them stably.

Further, in order to further improve the efficiency of the irradiation step, the catalyst may be added at a concentration of 1,000 to 10,000 ppm to further improve the low molecular weight of the polysaccharide, thereby further reducing the viscosity of the citron extract have.

Typical examples of such a catalyst include peroxides, peroxyacetic acid, and titanium dioxide (TiO ₂ ). Preferably, the catalyst is decomposed into water and oxygen upon irradiation with radiation, By using acetic acid, the effect of reducing polysaccharides by irradiation can be further improved.

As described above, the Phellinus linteus extract that has undergone the radiation irradiation process has a low molecular weight pectin polysaccharide, which can remarkably improve the absorption ability of the polysaccharide due to skin introduction or ingestion, while lowering the viscosity, It is possible to manufacture cosmetic products or foods having excellent palatability and physical properties.

According to the method of the present invention, it is possible to efficiently extract the active ingredients contained in the millennium and to lower the viscosity of the polysaccharide contained in the millennium by using a combined process of low temperature high pressure extraction and irradiation. It is possible to produce an insecticide extract having excellent physical properties capable of improving the physiological activity by increasing the content of the perennial plant in the production of cosmetics or foods.

The Chennaiensis extract prepared by the above method has a low viscosity and is easy to be dispersed even when added in an amount exceeding 5%, and can be effectively used for cosmetics or foods because of its excellent physiological activity including low molecular weight polysaccharides.

In addition, the present invention provides a technical content of a cosmetic composition containing a tsunami extract prepared by the above method.

In particular, the cosmetic composition of the present invention may contain 5 to 50 parts by weight of the extract of Chenopodium albumol, 70 to 90 parts by weight of water, and 1 to 10 parts by weight of a moisturizer.

The moisturizing agent may be exemplified by glycerin, squalene, glucose, and the like. When the moisturizing agent is added, the dispersion stability of the cosmetic composition can be further increased, the preservative effect can be enhanced, It is possible to prevent the skin from becoming dry.

In addition, the cosmetic composition of the present invention may further comprise a solvent such as 1,2-hexanediol, butyleneglycol, ethylhexanediol and the like in order to improve the dispersion of the perennial herb extract and to further increase the content thereof, May be mixed in a proportion of 1 to 5% by weight to control the physical properties of the cosmetic composition.

In addition, the cosmetic composition of the present invention may further comprise additives such as vitamins, oil fractions, surfactants, whitening agents, sedatives, chelating agents, pigments, fatty acids, antioxidants, fragrances, preservatives, neutralizing agents, The cosmetic composition of the present invention can be prepared in various forms such as serum, lotion, oil, cream, essence, and the like.

Furthermore, the present invention provides a technical content of a tsunami-fresh food composition comprising the tsunami seed extract prepared by the above-mentioned method, wherein the tsunami seed preparation comprises 5 to 20 parts by weight of the Tsunamiecaster extract and 75 to 90 parts by weight of water .

In addition, the food composition of the present invention can be used to produce food having improved taste and flavor by addition of natural essential oil, and to be used for manufacturing various functional health foods such as drinks, capsules, and rings for inner beauty through oral administration .

Hereinafter, the present invention will be described in more detail with reference to examples.

The embodiments presented are only a concrete example of the present invention and are not intended to limit the scope of the present invention.

≪ Example 1 >

The extract of Chenille seeds was prepared by the method shown in Fig.

First, the raw material was washed with water using the stem and the fruit as a raw material, the washed raw material was put into a visible removal device, and the visibility was removed for 40 minutes. In order to remove the visible light from the raw material, a visible removing device capable of forming a vacuum in the internal accommodating space is used.

In the same manner as described above, the raw material without visible was washed with water and then passed through a drying device equipped with a conveyor belt, a circular tunnel-shaped housing and an air injection part to remove moisture, and the moisture-removed raw material was supplied to the pulverizer.

The mixture was prepared by milling the mill base material for 15 minutes by supplying water at 40 캜 to the grinder supplied with the raw material of the millennium, and the prepared millennium mixture was supplied to the mixing tank and continuously stirred to prepare a homogeneous mixture. The mixture was heated to a temperature of 40 to 50 캜 in the preparation of the mixture, and the viscosity was confirmed and water was additionally supplied to prevent aggregation formation. The crushing was carried out using a motor capable of high-speed rotation of 2 horsepower and a crusher equipped with a double-edged knife, and a mixing tank equipped with two impellers was used for the preparation of the mixture.

The mixture prepared in the above manner was fed to a coldstostatic press and then subjected to a high pressure treatment at a temperature of 50 ° C. and a pressure of 80 MPa. The mixture was filtered to obtain a 300 mesh filter net To obtain a tsunami extract.

The obtained extract was applied to a Co-60 gamma ray irradiation system (point source AECL, IR-79), and then a 2.5 MeV transformer type electron accelerator (ELV-8Model, EB Tech.) Was irradiated with electron beam at 14.5mA beam current. The absorbed dose of radiation was operated at 10kGy to lower the molecular weight of the polysaccharide and the low-molecular-weight treatment was applied to the ultrafiltration with a 150 mesh filter membrane. To obtain a citrus extract. The obtained extract was stored in a refrigerator at -20 ° C to prevent deterioration.

≪ Example 2 >

The extract was prepared in the same manner as in Example 1 except that the acetic acid was added at a concentration of 3,000 ppm and then irradiated with radiation.

≪ Comparative Example 1 &

The high-pressure pretreated mixture of the perennial plant of Example 1 was filtered through a 300-mesh screen to obtain a perennial extract.

≪ Comparative Example 2 &

The mixture of high-pressure and high-pressure water was treated by the same method as in Example 1, and filtered through a 300-mesh filter net to obtain a tsunami extract.

≪ Experimental Example 1 >

In order to analyze the extraction efficiency of the active ingredient contained in the manufactured tsunami extract, the components of the polyphenols and flavonoids contained in the Ts extract prepared by the method according to Example 1 and Comparative Example 1 were analyzed, Lt; / RTI > Component analysis was carried out using the Maksimovic method. The polyphenol component analysis was carried out using garlic acid (Sigma Aldrich) as a standard substance, and the absorbance was measured by measuring light with a wavelength of 725 nm. The analysis of flavonoid components was carried out by adding aluminum chloride hexahydrate to the extract of Hanwoo chrysanthemum extract and measuring absorbance by light of 430 nm wavelength.

Polyphenol (mg / g) Flavonoid (mg / g) Example 1 4.79 ± 0.19 1.23 + 0.07 Comparative Example 1 3.36 ± 0.21 0.94 + 0.11

As shown in Table 1, the content of the flavonoid and polyphenol contained in the quinternial plant extract of Example 1 prepared by the high-pressure extraction method was lower than that of the polyphenol and flavonoids by the method according to Comparative Example 1 And it was confirmed that the extraction efficiency was remarkably improved by the high pressure extraction.

<Experimental Example 2> Viscosity analysis and molecular weight analysis

In order to analyze the viscosity of the mushroom extract, the yield strength of the mushroom extract prepared by the method according to Example 1, Example 2, and Comparative Examples 1 and 2 was analyzed, and the results are shown in Table 2 below. The yield strength analysis was performed by measuring the shear stress at 20 ° C with varying shear rate from 0 to 300 s ^-1 at 20 ° C using a viscosity analyzer.

Yield (Pa) Example 1 16.39 ± 0.47 Example 2 13.27 ± 0.36 Comparative Example 1 21.09 ± 0.49 Comparative Example 2 22.67 ± 0.53

As shown in Table 2, all of the manufactured tsunami extracts showed the non-viscous flow behavior which was not influenced by Newton's law, with the fluidity index (n) of 0 <n <1, Was found to have the highest yielding power as compared to the Chenille seed extract of Comparative Example 1. As a result, it was confirmed that the viscosity was the highest.

On the other hand, it was confirmed that the yield strength of the mushroom extract of Example 2 was lower than that of Example 1, and it was confirmed that the viscosity decreased remarkably due to the reduction of the yield strength. Compared with Comparative Examples 1 and 2 It was confirmed that the viscosity was remarkably lowered.

As a result of the above-mentioned results, it was confirmed that the low molecular weight was promoted by irradiation and the viscosity was decreased, and it was confirmed that addition of the catalyst can further promote the low molecular weight.

In addition, the perilla seed extract prepared by the method according to Example 1, Example 2, and Comparative Examples 1 and 2 was lyophilized to prepare a millet seed powder, and 10 to 30 g of the powder was mixed with 100 mL of water at 20 DEG C And then vigorously stirred for 5 minutes to confirm the solubility. As a result, in the case of the method according to Comparative Example 1, agglomeration was observed from the case where 10 g of the powder was added, and when the method according to Comparative Example 2 Agglomeration was confirmed when 8 g of the powder was added. On the contrary, in the case of mixing the extract of the miltr. Chinensis prepared by the method according to Example 1, aggregation was confirmed when the amount of the extract was more than 16% by weight, but in the case of miltr. It is possible to confirm that coagulation occurs from the case of the coagulation.

From the above results, it was confirmed that the polymer was converted into a low molecular weight through irradiation with the irradiation of the method according to Example 1, and the viscosity was remarkably decreased. The solubility of the Cheonnyuncho extract prepared by the method according to Example 2 was remarkably high , Respectively.

As a result of the above-mentioned results, it was found that the extract of Chenille Seil extract prepared by the method of the present invention contains a higher concentration of the effective ingredient contained in the millenniace, due to the combined process of low temperature high pressure extraction and irradiation, It was confirmed that the polysaccharide contained in the millenniace is low-molecular-weight and low in viscosity, so that it can be effectively used in manufacturing cosmetics or foods.

Claims (5)

(a) mixing a raw material of a silkworm plant containing a perennial plant stem and a perennial plant fruit with water at 35 to 60 ° C, and then homogenizing the plant to prepare a perennial plant mixture; And
and (b) filtering the mixture of the tsunami seeds to prepare a tsunami extract. The method according to claim 1,
Wherein the step (a) further comprises the step of pressurizing the mixture at a pressure of 30 to 100 MPa after preparing the mixture. 3. The method of claim 2,
Wherein the step (b) further comprises the step of irradiating the tubercanthopteran extract with radiation after preparing the tubercantholate extract. A cosmetic composition according to any one of claims 1 to 3, which comprises 5 to 20 parts by weight of an extract of Cheilinaceae, 70 to 90 parts by weight of water and 1 to 10 parts by weight of a moisturizer. A composition according to any one of claims 1 to 3, wherein the composition comprises 5 to 20 parts by weight of a quinch-mill extract and 75 to 90 parts by weight of water.

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