KR102151045B1 - Cosmetic Composition Having Anti-Inflammation and Skin-Irritation Mitigating Effect Containing Catalpa ovata and Vitex trifolia L. Mixed Extract - Google Patents

Abstract

The present invention relates to a cosmetic composition having anti-inflammatory and skin irritation alleviating effects containing a mixed extract of Catalpa ovata and Vitex trifolia L. as an active ingredient, and more specifically, Catalpa ovata And it relates to a cosmetic composition containing a 1:1 mixed extract of Manhyeongja (Vitex trifolia L.) as an active ingredient.

Description

Cosmetic Composition Having Anti-Inflammation and Skin-Irritation Mitigating Effect Containing Catalpa ovata and Vitex trifolia L. Mixed Extract}

The present invention relates to a cosmetic composition having anti-inflammatory and skin irritation alleviating effects containing a mixed extract of Catalpa ovata and Vitex trifolia L. as an active ingredient, and more specifically, Catalpa ovata And it relates to a cosmetic composition containing a 1:1 mixed extract of Manhyeongja (Vitex trifolia L.) as an active ingredient.

Cosmetics are applied to the skin by a normal person for beauty and health, and there are dozens of types of cosmetics. There are also various types of cosmetics that are used by ordinary people until they wake up and go to bed, and since they are constantly covering their skin while they are active, they have a very close relationship with humans.

In recent years, the ozone layer is destroyed due to global warming, climate change occurs frequently, and the human skin is also becoming sensitive due to the residential environment due to interiors containing toxic chemicals, and the eating habits that prefer instant food due to busy daily life.

As the number of people with sensitive skin increases, the demand for irritating cosmetics is also increasing. Cosmetics contain as few as 20 to 50 ingredients, but recently, as the functionality is emphasized rather than the safety of the ingredients, the ingredients used may cause problems such as irritation or inflammation on sensitive skin.

Looking specifically at skin problems that can occur due to the use of cosmetics, there are irritation sensations such as itching, tingling, and burning, and inflammatory problems such as erythema and edema.

The cause of skin side effects caused by cosmetics is that cosmetics penetrate into the skin and interact with epidermal or dermal cells, thereby activating the immune system and causing irritation and inflammatory reactions.

Such inflammation is a defense action of biological tissues against foreign substances, and physiological reactions such as activation of inflammatory mediators and immune cells present in local blood vessels or body fluids, secretion of inflammatory mediators, tissue destruction, and erythema, swelling, fever, and pain. It appears as an external symptom such as the back.

Regarding external stimuli, major substances that mediate the inflammatory response include nitric oxide (NO), histamine secreted from mast cells, and prostaglandin. Several cytokines are involved in generating these inflammatory mediators. Meanwhile, interleukin-6 and 8 (interleukin-6, interleukin-8: IL-6, IL-8) are inflammatory cytokines in mononuclear cells, fibroblasts, endothelial cells, and keratinocytes, and may induce histamine release in mast cells. .

The inventors of the present invention are conducting research to develop a cosmetic that is effective in relieving skin inflammation and irritation by not causing irritation to the skin and at the same time suppressing the secretion of inflammatory mediators by using natural products with guaranteed safety, Manhyeongja (Vitex trifolia L .) And it was confirmed that the mixed extract of catalpa ovata has a very excellent skin inflammation and irritation relief effect, and the present invention was completed.

It is an object of the present invention to provide a cosmetic composition having an excellent anti-inflammatory effect from natural materials with few side effects.

Another object of the present invention is to provide a cosmetic composition having excellent skin irritation alleviation effect from natural materials with few side effects.

All of the above and other objects of the present invention can be achieved by the present invention described in detail below.

The cosmetic composition according to the present invention is characterized in that it contains a mixed extract of Catalpa ovata and Manhyeongja (Vitex trifolia L.) as an active ingredient.

The mixed extract includes a 1:1 mixture of Catalpa ovata vs. Manhyeongja (Vitex trifolia L.).

The present invention has the effect of the invention to provide a cosmetic composition containing a mixed extract of Catalpa ovata and Manhyeongja (Vitex trifolia L.) as an active ingredient.

The present invention has the effect of the present invention to provide a cosmetic composition having excellent skin inflammation alleviation effect.

In addition, the present invention has the effect of the invention to provide a cosmetic composition having excellent skin irritation alleviation effect.

A cosmetic composition according to a preferred embodiment of the present invention includes a mixed extract of Catalpa ovata and Manhyeongja (Vitex trifolia L.) as an active ingredient. The extract is obtained by extracting from various organs or parts of each plant, and includes all extracts such as leaves, flowers, roots, stems, branches, shells, and seeds.

Hereinafter, the present invention will be described in more detail.

Catalpa ovata is a tree of the Jacaranda family and is also called Japanese cypress. It is native to China and is planted in gardens in various places in Korea. It grows up to 20m tall, flowers bloom around June, and long, narrow, linear fruits bear.

Paulownia tree is known as a kind of plant that produces iridoids such as catalposide and catalpol, and it has been found to have anti-inflammatory effects in oriental medicine, and is used to treat rheumatoid arthritis (J Ethno pharmacol. 2003;88:287-291).

Manhyeongja (Vitex trifolia L.) is the fruit of the sprout tree of the family Verbena. Sunbigi tree, the plant of the origin of Manhyeongja, has a unique and strong scent, and has been used as a material for bathing or as a moisture absorbent and antiseptic to remove indoor moisture. It was said that the private sector used it as a pillow.

In addition, in oriental medicine, including Korea, Chinese and Japanese, full-length people are cold, soothing, analgesic, anti-inflammatory because they are cold, chronic otitis media, tinnitus, hearing loss, vision impairment, headache, neuralgia, eczema. And various inflammatory and allergic diseases (Park JH and Lee CK (2000) The Encyclopedia of Medicinal Plants. Shinilbooks. 183-184, 248-249).

Recently, a paper was published that verified the anti-inflammatory effect by separating iridoid glucoside (J Nat Med. 2018 Feb: 1-6).

As described above, a number of natural compounds of the Iridoid family are contained in Paulownia and Manhyeongja, and the anti-inflammation effect on these substances has also been proven, but it has been added to cosmetics so far to relieve inflammation and irritation. Thesis or patents cannot be found.

The paulownia and barnacles used in the present invention can be purchased in Korea or China. In the present invention, the extract of the paulownia tree may be an extract of outpost, bark, root or leaf, preferably an extract of bark.

On the other hand, the Manhyeongja extract may be an extract of a fruit, a peel, a root or a leaf, and is preferably an extract of a fruit.

The mixed extract of Vitex trifolia L. and Catalpa ovata according to the present invention is used after cleanly washing the bark and fruit of bark of Paulownia with purified water, and may be used as it is, depending on the extraction method. It is preferable to use it by crushing.

The mixed extract of Vitex trifolia L. and Catalpa ovata of the present invention is a conventional method known in the art using water, a lower alcohol or glycol having 1 to 6 carbon atoms, or a mixed solvent thereof. It may be extracted according to, but preferably, it can be extracted by the following method.

After crushing the dried bark of Paulownia and the fruit of Manhyeongja finely, they are mixed in a weight ratio of 1:1, and washed thoroughly with purified water. The washed mixture was added 10 to 15% by weight to 40% 1,3-butylene glycol aqueous solution, and extracted for 2 to 10 hours with an ultrasonic extractor at room temperature.

The extract can be obtained according to a method known in the art, for example, Whatman filter paper, a filter device with 100 to 400 mesh, etc. attached, a vacuum filter device, etc., or agitation or rotation by a centrifugal separator. It can be obtained after separating the solid by a method such as.

According to the present invention, the mixed extract of Vitex trifolia L. and Catalpa ovata is an inducible monoxide that mediates the skin inflammatory response and relieves cytotoxicity by lactic acid, a substance that is a commonly used skin irritant. It showed excellent effect in inhibiting the secretion of nitrogen, IL-6, IL-8, PGE ₂ and histamine. The skin irritation relief effect was also excellent in the results of the human skin patch test.

Therefore, the mixed extract of Manhyeongja (Vitex trifolia L.) and Catalpa ovata of the present invention can be used in a cosmetic composition having an anti-inflammatory effect and an anti-irritation effect.

Meanwhile, the mixed extract of Manhyeongja (Vitex trifolia L.) and Catalpa ovata of the present invention may contain 0,01 to 100.0% by weight with respect to the entire cosmetic composition, and preferably 0.01 to 30% by weight. have. This is because if it is less than 0.01%, there is no effect, and if it exceeds 30.0%, the stability of the formulation decreases.

In addition to the mixed extract of Vitex trifolia L. and Catalpa ovata, which are active ingredients, the cosmetic composition of the present invention includes ingredients commonly used in cosmetic compositions, such as antioxidants, stabilizers, solubilizers , Conventional auxiliaries such as vitamins, pigments and flavors, and carriers.

The cosmetic composition according to the present invention is not particularly limited in its formulation, and is formulated as an emulsion, cream, essence, serum, gel, lipstick, foundation, gel, shampoo, conditioner, treatment, body cleanser, sunscreen, etc. Can be manufactured.

Hereinafter, the present invention will be described in more detail through Examples, Test Examples and Formulation Examples, but the scope of the present invention is not limited to the following Examples and Experimental Examples.

Example 1. Preparation of mixed extract of Paulownia and Manhyeongja

After crushing 50 g of dried dog paulownia bark and 50 g of bark fruit finely, mix, and wash thoroughly with purified water. The washed mixture was added to 1000 ml of 40% 1,3-butylene glycol aqueous solution, and extracted for 3 hours with an ultrasonic extractor at room temperature. After separating the precipitate using a centrifuge, the filtrate was filtered again with a 100 mesh filter to obtain a mixed extract.

Experimental Example 1. Soothing effect from skin cell stimulation by lactic acid

In order to confirm whether the extract of Paulownia and Manhyeongja of the present invention has a soothing effect on skin irritation, a cell stimulation alleviation experiment with lactic acid was performed.

Human skin cells, fibroblasts, were cultured in a T-75 flask (Falcon, USA) containing DMEM (sigma) until 80% growth, and then again in a 96-well plate containing DMEM (Falcon, USA). Incubated for 12 hours until 1 × 10 5 cells/well. After culturing the cells, it was confirmed through a microscope whether the cells were completely attached to form well, and the experiment was conducted as follows using 0.2% lactic acid as a skin cell irritant.

At the concentration of lactic acid, all normal skin cells are generally killed. 200 μl of DMEM containing 0.2% lactic acid was added to each of 96 wells of the plate in which fibroblasts were cultured, followed by 50, 100, 200, 500, and 1,000 μg of Paulownia and Manhyeongja extracts prepared in Example 1, respectively. /Ml was added.

As a control group, none of the extracts of Paulownia and Manhyeongja were added, and those containing only lactic acid and only the mixed extract of Paulownia and Manhyeongja were separately prepared as a control group.

After 12 hours of addition of the test substance, 10 μg of MTT ([3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) reagent was added to each well to compare the viability of cells. After each addition and leaving for 4 hours, the absorbance of each well was measured using an ELISA reader (Molecular Dervices), and the cell viability was measured by Equation 1 as follows, and the results are shown in Table 1 below. .

Concentration of mixed extract
(㎍/mL) Cell viability (%) Mixed extract Mixed extract + lactic acid Lactic acid 50 97 35 0 100 98 50 0 200 97 65 0 500 98 89 0 1000 98 92 0

As shown in Table 1, the extracts of Paulownia and Manhyeongja of the present invention had little cell stimulation and did not affect the survival of cells. In addition, the death of all cells by lactic acid increased the survival rate of cells as the treatment concentration of the extracts of Paulownia and Manhyeongja increased.

Accordingly, it can be confirmed that the extract of Paulownia and Manhyeongja of the present invention has the effect of suppressing skin cell death by mitigating skin cell stimulation by lactic acid.

Experimental Example 2. PGE ₂ And IL-6, IL-8 production inhibition measurement.

Human fibroblasts were inoculated in a 6-well culture plate at a concentration of 1 x 10 ⁵ cells and cultured in a 37°C 5% CO ₂ incubator for 24 hours. H ₂ O ₂ 500 μm was treated in a well to stimulate for 24 hours, and then the mixed extract of the present invention (Example 1) was treated by concentration, reacted for 48 hours, and the culture solution was collected and analyzed by ELISA.

PGE ₂ was used as a kit from AssayDesign, and for IL-6 and IL-8, a kit from Endogen was used. Experiments were conducted according to the manuals of each company.

The measurement of the inhibitory effect of this experimental example was calculated by Equation 2 below. The results are shown in Table 2. The control was a group treated with only the extraction solvent.

Configuration PGE ₂ pg/ml
(Inhibiting effect) IL-6 pg/ml
(Inhibiting effect) IL-8 pg/ml
(Inhibiting effect) Control 78.5 29.7 38.3 H ₂ O ₂ (500㎛) 315.1 184.2 329.7 H ₂ O ₂ (500㎛) + Example 1 (25㎍) 200.3 (48.5%) 118.5 (42.5%) 182.0 (50.7%) H ₂ O ₂ (500㎛) + Example 1 (50㎍) 153.9 (68.1%) 93.5 (58.7%) 100.9 (78.5%) H ₂ O ₂ (500㎛) + Example 1 (100㎍) 102.8 (89.7%) 43.3 (91.2) 59.3 (92.8%)

As a result of the experiment, it was confirmed that the amount of inflammatory mediators PGE ₂ , IL-6, and IL-8 was significantly reduced by the addition of the mixed extract, showing a high inhibitory effect. In particular, when the concentration of the mixed extract 100 ㎍ / ㎖ treatment, it was confirmed that the PGE ₂ , IL-6 and IL-8 showed an inhibitory effect of about 90%.

Experimental Example 3. Measurement of NO secretion inhibitory effect

In order to evaluate the skin inflammation alleviation effect of the mixed extract according to the present invention, the change in the secretion amount of nitric oxide (NO) was measured.

Raw 264.7 cells, which are macrophages from mice, were added to DMEM (Dulbecco's Modified Eagle's Medium) medium with 10% FBS (fetal bovineserum), 100 units/ml penicillin and 100 µg/ml streptomycin in an incubator (37°C). , 5% CO ₂ ).

When the cells grew to 80% of the bottom of a 100 mm dish, in order to induce an inflammatory response of the cells, a lipopolysaccharide was added to a DMEM culture medium containing 1% FBS at a concentration of 100 ng/ml.

Raw 264.7 cells were cultured for 24 hours by adding the extract or lipid polysaccharide obtained in Example 1, and the culture solution was collected and the amount of NO secretion was measured with a Griess reagent system (Kim JK et al., Biochem Biophys). ResCommun., 345, pp1215-1223, 2006), and the obtained results are shown in Table 3 below.

Mixed extract concentration (㎍/㎖) Control 10 50 100 200 NO secretion (% compared to control group) 100 69 51 25 12

As shown in Table 3, when the extract of the present invention was treated, NO production induced by lipopolysaccharide was reduced in a concentration-dependent manner. Through these results, it is expected that the use of the extract of the present invention can effectively alleviate skin inflammation.

Experimental Example 4. Evaluation of suppression of histamine secretion by mixed extract

Beta-hexosaminidase (β-hexosaminidase) is a substance constituting the granules of mast cells, and histamine secretion by degranulation of mast cells is proportional to the amount of beta-hexosaminidase released. Therefore, the inhibitory effect of the mixed extract on histamine secretion was confirmed by measuring the release amount of beta-hexosaminidase in RBL-2H3 cells, which is a rat mast cell line.

After suspending RBL-2H3 cells in DMEM containing 10% FBS, 2×10 ⁵ cells per well were dispensed into a 24 well plate, and then sensitized with 0.5 μg/ml DNP-IgE for each well and 5% CO ₂ Incubated overnight in an incubator. Then, the cells of each well were washed twice with Siraganian buffer (119 mM NaCl, 5 mM KCl, 5.6 mM glucose, 0.4 mM MgCl2, 25 mM HEPES, 40mM NaOH, 1 mM CaCl ₂ , 0.1% BSA, pH 7.2), and then Pre-reacted with Siraganian buffer at 37° C. for 10 minutes, and reacted again for 10 minutes after adding the test substance. Thereafter, cells were degranulated by treatment with antigen (DNP-BSA, 10 μg/ml) at 37° C. for 30 minutes, then placed on ice for 10 minutes to terminate the reaction, and 20 μl of the supernatant was transferred to a 96-well plate and then 1 mM After adding p-nitrophenyl-N-acetyl-β-D-glucosaminide and incubating for 1 hour, stop solution (0.1 M Na2CO3/NaHCO3) was added, and the absorbance was measured at 405 nm by ELISA and quantified.

As shown in Table 4 below, it was observed that when the extract of the present invention was treated, β-hexosaminidase was reduced in a concentration-dependent manner, and release was suppressed to about 63% at a concentration of 100 μM.

Mixed extract concentration (㎍/㎖) Control 10 20 50 100 Beta-hexosaminidase secretion
(% of the control group) 100 81 75 53 37

Experimental Example 5: Measurement of skin irritation relief effect through skin patch test

In order to evaluate the skin irritation alleviating effect of the mixed extract according to the present invention, a cosmetic composition containing sodium dodecyl sulfate (SDS), a surfactant known as a skin irritation-causing substance, was prepared as shown in Table 5 below. The induction of irritation and skin reactivity were measured through the patch experiment, and the effect of reducing skin irritation by the surfactant was compared and measured.

Furtherance Formulation Example 1 (% by weight) Comparative Formulation Example 1 (% by weight) Control example (% by weight) Mixed extract 10 Sodium dodecyl sulfate 1.5 1.5 Sodium hyaluronate 5 5 5 Trihalose One One One Glycerol 3 3 3 Carbomer 0.3 0.3 0.3 Tocopheryl Acetate 1.5 1.5 1.5 lecithin 2 2 2 Aracel 165 1.5 1.5 1.5 Beeswax One One One Shea Butter 3 3 3 Stearic acid 3 3 3 Camellia oil 3 3 3 Distilled water To 100 To 100 To 100

Twenty women in their 20s to 30s who have never had hypersensitivity reactions to skin irritation in the past and who do not currently have skin diseases or skin allergies were studied. First, the test area was wiped with 70% ethanol and then dried. The prepared test material was dropped into a pin chamber (Finn chamber, 100×10, EPITEST, Finland) by 15 μg, and then hermetically patched on the inner part of the forearm of the test subject. After the patch was applied for 24 hours, the patch was removed, and the test site was marked with a marker pen. After 1 hour and 24 hours, respectively (1 hour, 2 hours after patch removal = 24 hours, 48 hours including patch attachment), using a magnifying glass (8MC-150, DAZOR, United States of America) to observe the presence or absence of erythema and edema I did.

The skin reaction was determined according to the regulations of the International Contact Dermatitis Research Group (ICDRG) (Table 6 below), and the average skin reactivity was calculated according to Equation 3 below, and the results are shown in Table 6.

sign score Evaluation standard - 0.0 No reaction ± 0.5 Faint or mild erythema + 1.0 Erythema, swelling, and papules with distinct but weak ++ 2.0 Pronounced erythema, papules and vesicles +++ 3.0 Severe erythema, blisters, crust formation

division
24 hours 48 hours Average skin reactivity
(n=20) ± + ++ +++ ± + ++ +++ Contrast One 0.42 Comparative Formulation Example 1 10 One 3 One 7.08 Formulation Example 1 6 2 3.33

As can be seen from Table 7 above, in the skin patch experiment, strong skin irritation was observed for Comparative Formulation Example 1 containing sodium dodecyl sulfate, and Formulation Example 1 containing the extract of the present invention showed an irritation relief effect.

Experimental Example 6. Skin soothing effect

After dividing the groups into 30 people per evaluation group for volunteers with severe skin problems, the nutritional creams of Formulation Example 2 and Comparative Formulation Example 2 in Table 8 below were used twice daily for 2 weeks for each group, as shown in Table 9 below. Based on the same score, skin trouble relief and soothing effects were evaluated.

Furtherance Formulation Example 2 (% by weight) Comparative Formulation Example 2 (% by weight) Mixed extract 10 Sodium hyaluronate 5 5 Trihalose One One Glycerol 3 3 Beta glucan 2 2 Carbomer 0.3 0.3 Tocopheryl Acetate 1.5 1.5 lecithin 2 2 Aracel 165 1.5 1.5 Beeswax One One Shea Butter 3 3 Stearic acid 3 3 Camellia oil 3 3 Distilled water To 100 To 100

Skin condition evaluation criteria grade 1. Skin trouble relief effect
Skin problems such as acne, pimples, and atopy worsen
Skin problems such as acne, pimples, and atopy worsen slightly
Skin problems such as acne, pimples, and atopy are the same as before use
Skin problems such as acne, pimples, and atopy are slightly improved
Skin problems such as acne, pimples, and atopy are greatly improved.
One
2
3
4
5 2. Skin soothing effect
Skin conditions such as erythema, dryness, itchiness, and foreign bodies become very sensitive
Skin conditions such as erythema, dryness, itchiness, and foreign body sensations become slightly sensitive
Skin conditions such as erythema, dryness, itchiness, and foreign body sensation are the same as before use
Skin conditions such as erythema, dryness, itchiness, and foreign body sensation are slightly calmed
Skin conditions such as erythema, dryness, itchiness, and foreign body sensation are very calming
One
2
3
4
5

Skin trouble relief effect Skin soothing effect Formulation Example 2 4.2 3.9 Comparative Formulation Example 2 3.3 2.8

As shown in Table 10, it was confirmed that the cosmetic composition containing the mixed extract of the present invention has excellent skin soothing and trouble relief effects compared to the cosmetic composition not containing the mixed extract.

As described above, the present invention has been described with reference to a preferred embodiment, but simple modifications or changes of the present invention can be easily implemented by those of ordinary skill in the art, and all such modifications or changes are the present invention. It can be seen as being included in the domain of.

Claims (6)

As an active ingredient containing a mixed extract of 1:1 weight ratio of Catalpa ovata and Manhyeongja (Vitex trifolia L.),
The Catalpa ovata extract is a bark extract, and the Vitex trifolia L. extract is a fruit extract,
The mixed extract is a cosmetic composition, characterized in that the extraction by adding a mixture of the crushed Paulownia bark and Manhyeongja fruit to 1,3-butylene glycol aqueous solution. The method of claim 1,
The cosmetic composition is a cosmetic composition, characterized in that the skin inflammation and skin irritation relief effect. delete The method of claim 1,
The mixed extract is a cosmetic composition, characterized in that 0.01 to 30% by weight based on the total weight of the cosmetic composition. delete The method of claim 1,
The cosmetic composition is a cosmetic composition, characterized in that any one formulation selected from the group consisting of emulsion, cream, essence, gel, lipstick, foundation, gel, shampoo, conditioner, treatment, body cleanser and sunscreen.