KR102147764B1 - Spingoliposome cosmetic composition with effect of proliferation of skin cells - Google Patents

Abstract

The present invention relates to a sphingo liposome cosmetic composition containing various physiologically active ingredients, and more particularly, to a sphingo liposome cosmetic composition having excellent skin cell proliferation effect by containing human stem cell culture solution or lactic acid bacteria fermentation extract as an active substance. will be.

Description

Spingoliposome cosmetic composition with effect of proliferation of skin cells}

The present invention relates to a sphingoliposome cosmetic composition containing various physiologically active ingredients, and more particularly, to a sphingoliposome cosmetic composition having excellent skin cell proliferation effect by containing human stem cell culture solution or lactic acid bacteria fermentation extract as an active substance. will be.

Stem cells are divided into embryonic stem cells derived from the inner cell mass of the blastocyst embryo, which is the early stage of development, and adult stem cells contained in each tissue of the adult body. Stem cell culture media are known to contain components that promote skin cell growth and regeneration, such as fibroblast growth factor (FGF) (Korean Patent No. 10-1032271).

Fermentation is known to increase safety by decomposing various harmful ingredients, and to increase the active ingredient and absorption rate by generating digestive products.In particular, in the case of lactic acid bacteria fermentation, vitamins B1, B2, pantothenic acid, B6, folic acid, which provide skin moisturizing and whitening effects, It is rich in carbohydrates, amino acids, and various minerals, and various natural enzymes such as saccharification enzymes and proteases produced during fermentation are known to increase the production and absorption of effective substances, thereby providing antioxidant, anti-aging, whitening, and skin moisturizing effects. .

It is important that these active ingredients are well delivered to the skin cells. In the case of cosmetics, it is common to solubilize or emulsify a hydrophobic component and a hydrophilic component using a surfactant. Technology development is required to increase the efficiency of delivering specific bioactive substances to skin cells.

Accordingly, the inventors of the present invention were researching to manufacture a cosmetic with excellent skin cell proliferation effect, and the fermented extract prepared by adding Astragalus, soybean containing active ingredients such as isoflavone and saponin during fermentation of lactic acid bacteria has effective skin cell proliferation. It was found that this lactic acid bacterium fermented extract or human stem cell culture medium was used as a main component of sphingosine, a cell membrane component, and a cosmetic composition made of sphingoliposomes had excellent efficacy in proliferating human skin cells. The invention was completed.

Accordingly, it is an object of the present invention to provide a cosmetic composition for sphingoliposomes having a human skin cell proliferation effect.

In order to achieve the above object, the present invention provides a sphingoliposome cosmetic composition containing as an active ingredient an extract fermented with lactic acid bacteria by adding Hawanggi or soybeans or a human stem cell culture solution.

The cosmetic composition according to the present invention is a sphingoliposome composition containing an extract fermented with lactic acid bacteria by adding Astragalus or soybeans or a human stem cell culture medium as an active ingredient and exhibits high skin cell proliferation efficacy.

1 is a graph showing an increase in the number of cells when a sphingo liposome composition comprising a fermented extract of lactic acid bacteria according to the content of Astragalus extracts as an active ingredient was investigated for the effect on the proliferation of human fibroblasts.
FIG. 2 is a graph showing an increase in the number of cells when a sphingoliposome composition comprising fermented lactic acid bacteria fermented extracts fermented for each type of lactic acid bacteria as an active ingredient was investigated for the proliferation of human skin fibroblasts.

Hereinafter, the present invention will be described in detail.

In the present invention, 1) preparing a human stem cell culture medium or lactic acid bacteria fermented extract as an active ingredient; And 2) through the step of making sphingo liposomes containing an active ingredient, it was confirmed that the proliferation effect of fibroblast cells, which is skin cells.

In the above step 1), in the case of a human stem cell culture solution as an active ingredient, a solution obtained by culturing human embryonic stem cells or adult stem cells is used.

In the above step 1), in the case of the fermented extract of lactic acid bacteria as an active ingredient, astragalus or soybeans are added and fermented with lactic acid bacteria to be extracted and used. In the case of lactic acid bacteria, Lactobacillus bifidum , including Lactobacillus-based lactic acid bacteria or Streptococcus thermopilus , may be used alone or in a mixture of two or more. Lactobacillus fermentation uses a medium containing milk, glucose, and yeast extract as the basic medium, and adds between 1% to 99% by weight of the extract extracted by mixing one or two types of purified water and ethanol for Astragalus or soybeans. It is used by adding, preferably between 25% by weight and 75% by weight, and most preferably between 50% by weight and 75% by weight. The extraction temperature of Astragalus or soybeans is preferably between 10°C and 121°C, and can be extracted for 15 minutes to 24 hours. Astragalus or soybean extract is added to the basic medium to inoculate lactic acid bacteria and fermented for about 1 to 1 year, preferably for 2 days, so that sufficient fermentation can occur between 4°C and 40°C. As a solvent for extracting the fermentation broth, one selected from the group consisting of purified water, 1,3-butylene glycol, ethanol, and glycerin may be used alone or in combination of two or more. Is preferably between 10 ℃ and 121 ℃, it can be extracted for between 15 minutes to 24 hours.

In step 2) above, spingosine, lecithin, and glycerin are mixed. Their weight ratio is 1-10:1-10:1-10, respectively, in order, and most preferably 5: 3:2. The mixture is heated and stirred between 70° C. and 80° C. for 20 to 30 minutes. It is prepared by adding between 10% by weight to 99% by weight of the human stem cell culture solution or the lactic acid bacteria fermentation extract prepared in step 1) to the mixture prepared by stirring.

Hereinafter, the present invention will be described in detail through non-limiting examples.

(Example 1) Preparation of Astragalus extract

After washing 1 kg of Jecheon-san Hwanggi with purified water, 10 times of purified water is added and heated at 121°C for 15 minutes. It was allowed to stand at room temperature, the temperature was lowered to 40°C or less, and filtered under reduced pressure using a paper filter paper to prepare an Astragalus extract that passed through the filter paper.

(Example 2) Preparation of lactic acid bacterium fermented extract containing Astragalus extract

Lactic acid bacteria used in fermentation proceeded to groups A, B, and C. Lactobacillus A group includes three types of Streptococcus thermopilus , Lactobacillus bulgaricus , and Lactobacillus fermentum , and Lactobacillus bifidum B group is Lactobacillus bifidum . Lactobacillus acidophilus ( Lactobacillus acidophilus ), Lactobacillus casei , Lactobacillus plantarum ( Lactobacillus plantarum ), Lactobacillus bulgaricus ( Lactobacillus bulgaricus dermophilus ) Rus ( Streptococcus thermopilus ) 5 kinds were mixed and used. With respect to the weight of the medium prepared in Example 1 using 20% by weight of milk, 2% by weight of glucose, and 0.3% by weight of yeast extract as a basic medium, the Astragalus extract was 25% by weight, 50% by weight, and 75% by weight. The lactic acid bacteria A, B, and C group lactic acid bacteria were inoculated at 0.1% by weight based on the weight of the medium in a medium prepared by adding the remainder to 100% by weight with purified water and fermented at 36°C for 48 hours. Purified water in the same ratio was added to the fermentation broth, filtered under reduced pressure using paper filter paper, and passed through the filter paper to prepare a fermented extract of lactic acid bacteria.

(Example 3) Preparation of sphingo liposomes containing fermented extract of lactic acid bacteria as an active ingredient

50g of phytospingosine, 30g of lecithin, and 20g of glycerin were mixed and stirred while heating at 75°C for 25 minutes. The mixture prepared by stirring was added to 900 g of the lactic acid bacteria fermented extract prepared in Example 2 with stirring at 200 rpm for 20 minutes, and prepared.

(Example 4) Preparation of sphingo liposomes containing human stem cell culture medium as an active ingredient

Instead of the lactic acid bacteria fermented extract in Example 3, 900 g of human stem cell culture solution was prepared in the same manner as in Example 3.

(Example 5) Effect of sphingo liposome composition containing fermented lactic acid bacteria extract as an active ingredient on proliferation of human skin fibroblasts

Human dermal fibroblasts that exist in the dermal layer of the skin to form collagen were cultured with DMEM (Gibco) maintenance culture solution added with 8% FBS, and then 10000 pieces were added to 96-well plates, and the sphingo prepared in Example 3 The liposome composition was added and incubated for 72 hours. 10 μl of EZ-Cytox (Enhanced cell viability assay kit) solution was added to each well, and absorbance was measured at 450 nm after 1 hour.

1 is a result of investigating the effect of a sphingo liposome composition containing a fermented extract of lactic acid bacteria according to the amount of Astragalus extract as an active ingredient on the proliferation of human skin fibroblasts. This is a graph showing an increase in the number of cells that is more than 30% higher than ascorbic acid used as a control.

Figure 2 is a result of investigating the effect of the sphingo liposome composition containing fermented lactic acid bacteria fermented extracts fermented for each type of lactic acid bacteria as an active ingredient on the proliferation of human skin fibroblasts, all cells higher than 30% of ascorbic acid used as a positive control It showed an increase in the number, and in particular, a sphingo-liposome composition containing a fermented extract of lactic acid bacteria fermented with Lactobacillus bifidum , a group B, as an active ingredient, is a graph showing the highest increase in cell number.

(Example 6) Preparation of a cream formulation cosmetic containing sphingo liposomes containing human stem cell culture medium as an active ingredient

Purified water 26.46g, human stem cell culture medium containing sphingo-liposome 10g, cyclopentasiloxane 1g, PEG/PP-18/18 dimethicone 1g, butylene glycol 1g, niacinamide 5g, diphenylsiloxyphenyltrimethicone 1g, Dimethicone/Vinyl Dimethicone Crosspolymer 1g, Dimethicone 1g, Sodium Chloride 1g, Sepil PEG/PP-10/1 Dimethicone 1g, Acetylhexapeptide-8 1g, Ginseng Callus Culture Extract 1g, Golden Callus Culture Solution 1g, baecho flavor callus culture solution 1g, camphor ginseng extract 1g, hydrolyzed placenta extract 1g, hollyhock root extract 1g, aloe vera leaf extract 1g, RH-polypepnide-7 1g, SH-oligopeptide-1 1g, copper tree Peptide-1 1g, SH-polypeptide-2 1g, Oligopeptide-28 1g, Oligopeptide-29 1g, Oligopeptide-30 1g, Oligopeptide-31 1g, SH-decapeptide-7 1g, SH-decapeptide 1g , SH-oligopeptide -9 1g, SH-pentapeptide-19 1g, Octapeptide-7 1g, Decapeptide-6 1g, Propolis extract 1g, Acaija extract 1g, Lactobacillus/bean fermentation extract 1g, White willow peel Extract 1g, Broiler Extract 1g, Oregano Leaf Extract 1g, Cypress Tree Leaf Extract 1g, Golden Extract 1g, Portulaca oleracea extract 1g, Alanine 1g, Glycine 1g, Arginine 1g, Serine 1g, Leucine 1g, Isoleucine 1g, Dipotassium Glycyrrhase Thurs 1g, Aloe Vera Leaf Juice Powder 1g, Adenosine 0.04g, Centellar Extract 1g, Octagonal Fennel Extract 1g, Tocopheryl Acetate 1g, Sorbitan Sesquioleate 1g, Caprylyl Glycol 1g, 1,2-hexanediol 1g, It was prepared by mixing 1 g of disodium EDTA, 1 g of sodium hyaluronate, 1 g of allantoin, and 0.5 g of fragrance.

Claims (4)

Contains sphingo liposomes prepared by stirring and adding a mixture of spingosine, lecithin, and glycerin to the fermented extract of lactic acid bacteria,
The mixture is a mixture of sphingosine, lecithin and glycerin in a weight ratio of 5:3:2,
The lactic acid bacteria fermented extract is a cosmetic composition for improving wrinkles, characterized in that fermented with Lactobacillus bifidum by adding 25 to 75% by weight of Astragalus extract.
delete delete The method of claim 1,
The cosmetic composition,
A cosmetic composition for improving wrinkles, characterized in that it has a formulation of suspension, emulsion, paste, gel, cream, lotion, powder, wax or spray.

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