KR102128957B1 - 조절가능한 프로모터 - Google Patents

조절가능한 프로모터 Download PDF

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KR102128957B1
KR102128957B1 KR1020197005781A KR20197005781A KR102128957B1 KR 102128957 B1 KR102128957 B1 KR 102128957B1 KR 1020197005781 A KR1020197005781 A KR 1020197005781A KR 20197005781 A KR20197005781 A KR 20197005781A KR 102128957 B1 KR102128957 B1 KR 102128957B1
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promoter
seq
nucleotide sequence
poi
pastoris
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KR20190025048A (ko
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디트하드 마타노비치
브리지트 개서
마이클 마우러
롤랜드 프리엘호퍼
요아힘 클라인
자나 웽거
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론자 리미티드
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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • CCHEMISTRY; METALLURGY
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0008Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
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    • C12YENZYMES
    • C12Y102/00Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01012Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) (1.2.1.12)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55Fab or Fab'
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

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  • Health & Medical Sciences (AREA)
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  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
KR1020197005781A 2011-10-07 2012-10-05 조절가능한 프로모터 Active KR102128957B1 (ko)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201161544451P 2011-10-07 2011-10-07
EP11184323 2011-10-07
US61/544,451 2011-10-07
EP11184323.1 2011-10-07
EP12171006 2012-06-06
EP12171006.5 2012-06-06
PCT/EP2012/069757 WO2013050551A1 (en) 2011-10-07 2012-10-05 Regulatable promoter

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KR1020147009284A Division KR101954389B1 (ko) 2011-10-07 2012-10-05 조절가능한 프로모터

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KR20190025048A KR20190025048A (ko) 2019-03-08
KR102128957B1 true KR102128957B1 (ko) 2020-07-02

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KR1020197005781A Active KR102128957B1 (ko) 2011-10-07 2012-10-05 조절가능한 프로모터
KR1020147009284A Active KR101954389B1 (ko) 2011-10-07 2012-10-05 조절가능한 프로모터

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US (4) US9512432B2 (enExample)
EP (2) EP2764016B1 (enExample)
JP (1) JP6113736B2 (enExample)
KR (2) KR102128957B1 (enExample)
CN (3) CN103998460A (enExample)
AR (1) AR088252A1 (enExample)
AU (1) AU2012320421B2 (enExample)
BR (1) BR112014008152A2 (enExample)
CA (1) CA2849856C (enExample)
DK (1) DK3508494T3 (enExample)
ES (1) ES2913825T3 (enExample)
IL (1) IL231859B (enExample)
IN (1) IN2014DN03419A (enExample)
PL (1) PL2764016T3 (enExample)
SG (2) SG11201400811QA (enExample)
TW (1) TWI560272B (enExample)
WO (1) WO2013050551A1 (enExample)

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WO2013050551A1 (en) * 2011-10-07 2013-04-11 Lonza Ltd Regulatable promoter
CN104195165A (zh) * 2014-09-01 2014-12-10 北京爱必信生物技术有限公司 包含葡萄糖启动子的载体、宿主菌以及重组牛源胰蛋白酶的制备方法
WO2016077457A1 (en) 2014-11-11 2016-05-19 Clara Foods Co. Methods and compositions for egg white protein production
WO2017021541A1 (en) * 2015-08-05 2017-02-09 Lonza Ltd Promoter variants
WO2017149065A1 (en) 2016-03-02 2017-09-08 Lonza Ltd Improved fermentation process
CN110088279A (zh) * 2016-12-15 2019-08-02 株式会社钟化 新型宿主细胞及使用了其的目标蛋白质的制造方法
WO2019154686A1 (en) 2018-02-12 2019-08-15 Lonza Ltd Host cell for producing a protein of interest
EP3536784A1 (en) 2018-03-05 2019-09-11 ACIB GmbH Host cell engineered for improved metabolite production
US11866714B2 (en) 2018-06-07 2024-01-09 Basf Se Promoter for yeast
DK3874047T4 (da) 2019-01-11 2025-02-03 Lonza Ag Karbonkilde-reguleret proteinproduktion i en rekombinant værtscelle
DK3690052T5 (da) * 2019-01-29 2024-08-26 Clariant Produkte Deutschland Gmbh Kimær promotor til anvendelse i metabolsk manipulation
WO2020200414A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Protein production in mut-methylotrophic yeast
EP3947695B1 (en) 2019-04-01 2023-09-06 Universität für Bodenkultur Wien Mut- methylotrophic yeast
WO2020200415A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Mut- methylotrophic yeast
DK3997118T3 (da) 2019-07-11 2025-03-24 Clara Foods Co Drikkevaresammensætning, der indeholder rekombinant ovomucoid-protein
US12096784B2 (en) 2019-07-11 2024-09-24 Clara Foods Co. Protein compositions and consumable products thereof
US10927360B1 (en) 2019-08-07 2021-02-23 Clara Foods Co. Compositions comprising digestive enzymes
AU2021240417A1 (en) 2020-03-26 2022-10-20 AskBio Inc. Forskolin-inducible promoters and hypoxia-inducible promoters
ES3034258T3 (en) 2020-04-01 2025-08-14 Lonza Ag Helper factors for expressing proteins in yeast
EP4136237A1 (en) 2020-04-15 2023-02-22 Asklepios Biopharmaceutical, Inc. Forskolin-inducible promoters and hypoxia-inducible promoters
EP4222266A1 (en) 2020-09-30 2023-08-09 Lonza Ltd. Host cells overexpressing translational factors
US20240141363A1 (en) 2021-02-12 2024-05-02 Boehringer Ingelheim Rcv Gmbh & Co Kg Signal peptides for increased protein secretion
WO2024133538A1 (en) 2022-12-20 2024-06-27 Lonza Ltd Host cells with pat1 knockout for increased specific protein productivity
WO2025247966A1 (en) 2024-05-29 2025-12-04 Mybios Gmbh Process for protein production in methylotrophic yeast host cell.

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WO2006089329A2 (en) 2005-02-23 2006-08-31 Technische Universität Graz Mutant aox 1 promoters

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WO2006089329A2 (en) 2005-02-23 2006-08-31 Technische Universität Graz Mutant aox 1 promoters

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Nucleic Acids Research. Vol. 36, No. 12, e76 (2008.06.06.)

Also Published As

Publication number Publication date
IN2014DN03419A (enExample) 2015-05-22
KR101954389B1 (ko) 2019-03-06
JP6113736B2 (ja) 2017-04-12
TW201317350A (zh) 2013-05-01
US11976284B2 (en) 2024-05-07
SG10201602673PA (en) 2016-05-30
AU2012320421A1 (en) 2014-03-20
DK3508494T3 (da) 2022-05-23
EP2764016A1 (en) 2014-08-13
EP3508494B1 (en) 2022-02-23
IL231859B (en) 2020-08-31
CN110055248A (zh) 2019-07-26
WO2013050551A1 (en) 2013-04-11
AR088252A1 (es) 2014-05-21
US20220259608A1 (en) 2022-08-18
KR20140069112A (ko) 2014-06-09
BR112014008152A2 (pt) 2017-04-18
US20170037418A1 (en) 2017-02-09
US9512432B2 (en) 2016-12-06
SG11201400811QA (en) 2014-08-28
US10301634B2 (en) 2019-05-28
US11401523B2 (en) 2022-08-02
JP2014530016A (ja) 2014-11-17
CN113788884B (zh) 2024-07-23
CA2849856A1 (en) 2013-04-11
TWI560272B (en) 2016-12-01
US20190185866A1 (en) 2019-06-20
EP2764016B1 (en) 2019-03-13
US20140242636A1 (en) 2014-08-28
PL2764016T3 (pl) 2019-08-30
KR20190025048A (ko) 2019-03-08
ES2913825T3 (es) 2022-06-06
CA2849856C (en) 2023-03-14
IL231859A0 (en) 2014-05-28
CN103998460A (zh) 2014-08-20
CN113788884A (zh) 2021-12-14
EP3508494A1 (en) 2019-07-10
AU2012320421B2 (en) 2017-03-02

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