KR102124400B1 - Cosmetic composition for cleansing fine dust comprising bioconversion products of asparagus cochinchinensis and soybean embryoas by liquid cultivation of basidiomycetes mycelium - Google Patents

Abstract

The present invention relates to a cosmetic composition for cleansing skin, comprising a bioconversion product of Asparagus cochinchinensis Merr. and soybean gemmule by a liquid culture process of basidiomycota mycelium and, more specifically, to a cosmetic composition comprising a bioconversion product as an active ingredient, wherein the bioconversion product is excellent in cleaning power of fine dust and has excellent skin moisture content improvement and keratin improvement effects.

Description

COSMETIC COMPOSITION FOR CLEANSING FINE DUST COMPRISING BIOCONVERSION PRODUCTS OF ASPARAGUS COCHINCHINENSIS AND SOYBEAN EMBRYOAS BY LIQUID CULTIVATION OF MYCEDIOM

The present invention relates to a cosmetic composition for cleaning skin comprising bioconversion products of astronomical dong and soybean embryos by a liquid culture process of basidiomycete mycelia, more specifically, excellent in fine dust cleaning power, improving skin moisture content, improving efficacy and keratin improvement It relates to a cosmetic composition comprising the bioconversion product excellent in efficacy as an active ingredient.

Schizophyllum commune is a taxonomically classified fungus belonging to the genus Pleurotus eryngii and Skirt fungi according to The Agaricales in modern taxonomy (1975), Beta-1,6 -Branch-beta-1,3-glucan (β-1,6-branched-β-1,3-glucan) is a strain that produces extracellularly. Skirt mushrooms can be collected in the wild and can be identified by the following morphological characteristics or taxonomic indicators. The fruiting body of the skirt mushroom has no stand, the side of the lampshade is attached to the substrate, and the size is usually 1.0 to 3.0cm, the shape is fan-shaped or clam-shaped, wrinkled with bells, and the ends are irregularly cracked and covered with fine hairs. Wrinkles are white, but when they mature, they have a light gray or pale brown color, and the tissue contracts when it is dry and recovers when it absorbs moisture. The spores are white, and the spores are conical, 4~6 X 1.5~2㎛, smooth and white. Schizophyllum commune Fr. is a keratinous mushroom native to old trees, etc. More detailed regime and shape are described in detail in the Korean Mushroom Book and Singer's classification.

Skirt mushrooms were known to be edible only in China due to the rough texture, but the bioactivity of the polysaccharide called'Schizophyllan' of the β-1,6-branch-β-1,3-glucan structure present in this mushroom That is, as the immunological effects such as moisturizing effect, anti-tumor activity, macrophage stimulation, and anti-biotic activity (Shimizu et al. 1992; Komatsu et al. 1973) are known, it is effective to use drugs, cosmetics, etc. It is used in various ways as a functional ingredient. Schizophyllan derived from Schizophyllum is a glucan that has regular beta-1,6- residues in the main sugar chain of beta-1,3-glucan. The molecular weight of β-glucan produced from other mushrooms such as shiitake mushrooms (Lentinus edodes), oyster mushrooms (Pleurotus ostreatus), and Phellinus linteus is hundreds of thousands to 2 million, whereas the molecular weight of schizophyllan derived from skirt mushrooms It is considerably larger from 2 million to 5 million, and has a uniform and unique structure with a branched uniform and unique structure compared to β-glucan of other mushrooms having a heterogeneous sugar composition and structure. [Ref: Kenichi T., Saimei T. Synthesis of the repeating units of Schizophyllan. 1986].

As the function of beta-glucan, a polysaccharide derived from shiitake, is known, various approaches to pure separation tablets have been attempted. As a method for obtaining beta-glucan derived from skirt mushrooms, the mushroom fruiting body has a very long cultivation period, and there is a possibility that the composition is not uniform depending on the cultivation method, and the mycelium cultivation by the solid medium also has a long cultivation period and is not uniformly made. When extracting beta glucan, the influence of extraction by the medium component cannot be excluded. Accordingly, as a method for obtaining a large amount of beta glucan, a bioactive component of the shiitake, in a short period of time, the mycelium obtained from the fruiting body is liquid cultured. As a method for efficiently obtaining pure high-concentration beta-glucan using mushroom mycelia, a lot of increase in production by liquid culture has been studied, and in particular, research on beta-glucan generated extracellularly is actively conducted on situational mushrooms, ganoderma etc. It has been.

On the other hand, Asparagus cochinchinensis (Lour.) Merr. is a Liliaceae plant distributed in Korea, China, and Japan, and has been widely used for medicinal and medicinal purposes since ancient times. Various pharmacological effects are known, including action, antitussives, antibacterial, neurostable, salivation, and antipyretic.

Accordingly, it has been used for the treatment of inflammatory diseases related to hepatitis, dermatitis, asthma, diabetes, and brain diseases along with additional effects such as sedatives and stabilizers. 19 amino acids and polysaccharides, as well as 20 multifunctional compounds (β-sitosterol, daucosterol, n-ethatriacontanoic acid, palmitic acid, 9-heptacosylene, smilagenin, diosgenin, sarsasapogenin-3-O-β-D- glucoside feeding grapes imidacloprid, 5-methoxy methylfurfural, yame sapogenin, diosgenin-3-O-β-D imidacloprid feeding glucose glycosides, aspacochioside D, iso-agatharesinoside and seven steroidal saponins). In addition, the effect of inhibiting apoptosis and suppressing inflammation induced by the skin of rats was confirmed, and it is known to promote osteoblast differentiation and inhibit osteoclast production in relation to bone metabolism.

[Nutrition component of Tianmun-dong]

Soybeans are rich in protein and fat, and contain a lot of essential amino acids, lysine. Protein 20~45%, fat 18~22%, carbohydrate 22~29%, ash 4.5~5%, contains essential amino acids lysine, cystine, tryptophan, glutamic acid, etc., among them, most suitable for Soybean Embryo It contains a lot of isoflavones (2%). In addition, soybean contains a large amount of saponin, which prevents the formation of lipid peroxide in the body and participates in lipid metabolism to effectively prevent aging and obesity. Saponin contained in soybean has a total of 5 types (Soyasaponin Ⅰ, Ⅱ, Ⅲ) , A ₁ , A ₂ ).

In the present invention, to provide a cosmetic composition for cleaning the skin comprising a bioconversion product obtained through an efficient bioconversion process (microbial fermentation conversion) using natural raw materials having various effects, astronomical dong and soybean embryo as a culture substrate.

Biocosmetics that contain substances made by applying biotechnology, such as microbial fermentation conversion, as a main component, are safer for skin by utilizing organic substances derived from natural conditions, unlike conventional cosmetics that are artificially synthesized using chemical technology. It is considered. In a broad sense, it may be used in combination with cosmetics containing natural ingredients, but it is differentiated in that it uses microbial fermentation, cell fusion, mass replication technology, etc. to mass-produce active ingredients and enhance safety and efficacy.

Republic of Korea Patent Publication No. 10-2017-0025066

The present invention is a natural raw material having a variety of efficacy cheonmundong and soybean germ as a culture substrate, basidiomycete mycelium liquid culture process (bioconversion process (microbial fermentation conversion)) obtained through the bioconversion product obtained as an active ingredient skin Provided is a cosmetic composition for cleaning.

In addition, the present invention provides a cosmetic composition for cleaning fine dust comprising a bio-conversion product obtained by inoculating basidiomycetes mycelia in a culture medium containing astronomical dong and soybean embryos and incubating them.

In addition, the present invention provides a cosmetic composition for improving keratin comprising a bio-conversion product obtained by inoculating basidiomycete mycelium in a culture medium containing astronomical dong and soybean embryos and incubating them.

In addition, the present invention provides a cosmetic composition for improving skin moisture content, comprising as an active ingredient a bioconversion product obtained by inoculating a basidiomycete mycelium in a culture medium containing astronomical dong and soybean embryos.

However, the technical problem to be achieved by the present invention is not limited to the problems mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

The present invention provides a cosmetic composition for cleaning the skin comprising a bioconversion product obtained by inoculating basidiomycete mycelia in a culture medium containing astronomical dong and soybean embryos and incubating them.

The astronomical dong and soybean embryos may be used in powder form.

The basidiomycete mycelia may be cultured in a liquid medium.

The basidiomycete mycelia may be obtained by culturing in a liquid medium for stationary for 5 to 7 days, followed by shaking and culture for 1 to 3 days.

The culture medium may include glucose, astronomical dong, soybean embryo, sodium nitrate, potassium phosphate, and magnesium sulfate.

The culture medium contains 65 to 75 parts by weight of glucose, 72 to 82 parts by weight of astronomical dong, 0.5 to 5 parts by weight of soybean embryos, 15 to 25 parts by weight of sodium nitrate, 10 to 20 parts by weight of potassium phosphate, and 1 to 7 parts by weight of magnesium sulfate can do.

The culture can be carried out by inoculating 5-15% (v/v) of Basidiomycetes.

The culture may be performed by shaking culture at 25~35 ℃ for 3~5 days.

The basidiomycete mycelia may include one or more selected from the group consisting of skirt mushrooms, circumference mushrooms, chaga mushrooms, shiitake mushrooms, leafy mushrooms, wood ear mushrooms and snowflake mushrooms.

The bioconversion product may include beta glucan, amino acids and flavonoids.

The bioconversion product may be included in an amount of 0.0001 to 20% by weight based on the total weight of the cosmetic composition.

In addition, the present invention provides a cosmetic composition for cleaning fine dust comprising bioconversion products obtained by inoculating basidiomycete mycelium in a culture medium containing astronomical dongs and soybean embryos and incubating them.

In addition, the present invention provides a cosmetic composition for improving keratin comprising a bio-converted product obtained by inoculating basidiomycete mycelium in a culture medium containing astronomical dong and soybean embryos and incubating it.

In addition, the present invention provides a cosmetic composition for improving skin moisture content, comprising as an active ingredient a bioconversion product obtained by inoculating a basidiomycete mycelium in a culture medium containing astronomical dong and soybean embryos.

According to the present invention, it is possible to provide a cosmetic composition for cleaning skin comprising bioconversion products obtained through a bioconversion process using a liquid culture process of basidiomycete mycelium based on astronomical dong and soybean embryos. The cosmetic composition according to the present invention is excellent in fine dust cleaning power, and is excellent in improving keratin and improving skin moisture content.

1 is an alternating relationship graph showing the effect of media components on beta-glucan productivity (measured by EPS value) and alternating factors in the results of regression analysis of FFD (A: soybean embryo, B: astronomical cavity, C:NaNO ₃ , D: KH ₂ PO ₄ ).
FIG. 2 is a graph showing DCW, EPS, Final pH, and Yp/x analysis results according to RSM (Response Surface Method) trial number.
FIG. 3 is a graph illustrating the effects of media factors on productivity in a three-dimensional graph according to the central synthesis planning method for RSM experiments, and analyzing the effects and interactions of media with beta glucan from the viewpoint of production.
4 is a graph showing the results of analyzing the interaction between media and beta glucan productivity according to the increase in astronomical dong according to the One Factor At a Time (OFAT) method.
5 is a graph showing the result of analyzing the change in the content of total flavonoids with increasing astronomical dong according to the OFAT (One Factor At a Time) method.
Figure 6 shows the astronomical dong / soybean embryonic (fermentation) conversion process performed in a 5L incubator.
7 is a disk fish analyzing the antimicrobial effect of cheonmun-dong hot water extract (A) astronomical dong / soybean germ (fermentation) conversion product (B), astronomical dong / soybean germ not added fermentation culture (C), control (D) It shows the results of the Dis assay.
Figure 8 shows the results of comparison of the amino acid content before and after the bioconversion process (microbial fermentation conversion) using natural raw materials Astronomical dong and soybean embryo as a culture substrate (amino acid control, astronomical dong hydrothermal extract, astronomical dong/soybean embryonic organism in order from the top ( Fermentation) conversion products).
9 is a graph showing the results of analyzing the fine dust cleaning power of a cosmetic composition for cleaning skin containing bioconversion products of astronomical sinus and soybean embryos.
10 is a graph showing the results of an analysis of the effect of immediate keratin improvement of a cosmetic composition for cleaning skin containing bioconversion products of astronomical sinus and soybean embryos.
11 is a graph showing the results of analyzing the skin moisture content improvement efficacy of a cosmetic composition for cleaning skin comprising bioconversion products of astronomical dong and soybean embryo.

Hereinafter, the present invention will be described in more detail through examples. The objects, features, and advantages of the present invention will be readily understood through the following examples. The present invention is not limited to the embodiments described herein, and may be embodied in other forms. The embodiments introduced herein are provided to sufficiently convey the spirit of the present invention to those skilled in the art to which the present invention pertains. Therefore, the present invention should not be limited by the following examples.

The present invention is a cosmetic composition for cleaning the skin comprising bioconversion products (or fermentation conversion products) of astronomical dongs and soybean embryos obtained from cultures inoculated with basidiomycete mycelia in a culture medium containing astronomical dongs and soybean embryos as an active ingredient. Provided is a composition. In the present invention, bioconversion products and fermentation conversion products are used in the same sense.

The composition has excellent fine dust cleaning power, and excellent in improving keratin and improving skin moisture content. Therefore, it can be applied as a cosmetic composition for cleaning fine dust, a cosmetic composition for improving keratin, and a cosmetic composition for improving skin moisture content.

The content of the bioconversion product in the cosmetic composition of the present invention is not particularly limited, and may be included in 0.0001 to 20% by weight based on the total weight of the composition. When the bioconversion product is less than 0.0001% by weight in the cosmetic composition, the dose may be small and thus may not have the expected effect, and when it exceeds 20% by weight, the change in effect may be insignificant.

The term "cosmetic composition" used in the present invention is a composition containing the compound, and the formulation may be in any form. For example, in the case of such a formulation, the cosmetic product prepared using the composition includes nutrient cream, eye cream, massage cream, creams such as cleansing cream, packs, lotions such as nutrient lotions, essences, softening lotion, and lotion such as nutrient lotions Types, powders, foundations, makeup bases, and the like, and may be manufactured and commercialized in any form of these formulations to achieve the object of the present invention, and are not limited to the above examples. In addition, the cosmetic composition according to the present invention can be formulated in a conventional cosmetic manufacturing method.

Specifically, the cosmetic of the present invention is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, pack, mask pack, mask sheet , Exfoliant, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, press powder, rust powder and eye shadow.

The cosmetic composition of the present invention may include other additives such as excipients and carriers in addition to the above-mentioned active ingredient (bioconversion product), and it is possible to apply and mix the usual ingredients blended into general skin cosmetics as necessary.

Specifically, the cosmetic composition of the present invention may further include a transdermal penetration enhancer. The term used in the present invention, transdermal penetration enhancer is a composition that allows the desired component to penetrate into the vascular cells of the skin with a high absorption rate. Preferably, other phospholipid components, liposome components, and the like used in lecithin cosmetics are included, but are not limited thereto.

In addition, one or more selected from vegetable oils, mineral oils, silicone oils and synthetic oils may be used as oils that can be mainly used as the oil component. More specifically, mineral oil, cyclomethicone, squalane, octyldodecyl myristate, olive oil, bitis vinifera seed oil, macadamia nut oil, glyceryl octanoate, castor oil, ethylhexyl isononanoate, dime Chicon, cyclopentasiloxane and sunflower seed oil can be used.

In addition, 0.1 to 5% by weight of surfactant, higher alcohol, and the like may be added to reinforce the emulsifying ability. Conventional surfactants such as nonionic surfactants, anionic surfactants, cationic surfactants, amphoteric surfactants, and phospholipids can be used as such surfactants. Specifically, sorbitan sesquinolate, polysorbate 60 , Glyceryl stearate, lipophilic glyceryl stearate, sorbitan oleate, sorbitan stearate, die-cetyl phosphate, sorbitan stearate / sucrose cocoate, glyceryl stearate / polyethylene glycol-100 Stearate, ceteareth-6 oleate, arachidyl alcohol/behenyl alcohol/arachidyl glucoside, polypropylene glycol-26-butes-26/polyethylene glycol-40 hydrogenated castor oil, etc. can be used. have. As the higher alcohol, an alcohol having 12 to 20 carbon atoms such as cetyl alcohol, stearyl alcohol, octyldodecanol, isostearyl alcohol, or the like may be used alone or in combination of one or more.

The aqueous phase component may further add 0.001 to 5% by weight of one or more thickeners such as carbomer, xanthan gum, bentonite, magnesium aluminum silicate, cellulose gum, dextrin palmitate, etc. in order to adjust the viscosity or hardness of the aqueous phase.

In addition, the cosmetic composition of the present invention, if necessary, high-level fatty acids, vitamins, and other active ingredients, sunscreens, and antioxidants (butyl hydroxyanisole, propyl gallic acid, elisoric acid, tocopheryl acetate, butylated hydroxy Toluene, etc.), preservatives (methylparaben, butylparaben, propylparaben, phenoxyethanol, imidazolidinylurea, chlorphenesin, etc.), colorants, pH adjusters (triethanolamine, citric acid, citric acid, sodium citrate, malic acid, Sodium malate, fumaric acid, sodium malate, succinic acid, sodium succinate, sodium hydroxide, sodium hydrogen phosphate, etc., moisturizers (glycerin, sorbitol, propylene glycol, butylene glycol, hexylene glycol, diglycerin , Betaine, glycereth-26, methylglucose-20, etc.), lubricants, and other components can be further added.

In addition, the cosmetic composition of the present invention further includes a substance capable of supplementally providing essential nutrients to the skin, and may preferably contain auxiliaries including but not limited to natural, cosmetic, or herbal medicines. have.

The present invention develops an efficient bioconversion process (or fermentation conversion process) of natural raw materials having various effects, astronomical dong and soybean embryo, as a culture substrate, and β- produced by the effective components of astronomical dong and soybean embryo and skirt mushroom ( schizophyllum commune ). A biological (fermentation) conversion product, which is a composite functional material that maximizes the productivity of glucan (β-glucan), was prepared and applied to a cosmetic composition.

The bioconversion process (or fermentation conversion process) according to the present invention can maximize the productivity of β-glucan produced by Efficacy components and Basidiomycete mycelia of astronomical dong and soybean embryo. More specifically, through the bioconversion system that achieves chemical changes of substances using the physiological functions of microorganisms ( Schophophlumlum commune ), it improves the toxicity and efficacy of the substances contained in herbal natural products (astronomical dong and soybean embryos) while simultaneously improving the efficacy and biocultivation process By controlling the metabolite through, there is an advantage in that beta-glucan, an active ingredient produced by microorganisms, can be produced high.

Schizophyllum commune used in the present invention is a basidiomycete and has no toxicity and produces beta-1,6-branch-beta-1,3-glucan (schizophyllan), an extracellular biopolymer. Schizophyllan, which is a beta-glucan derived from skirt mushroom, is a nonionic water-soluble polysaccharide with an average molecular weight of 2 to 5 X 10 ⁶ Da, and has anti-cancer capacity, anti-vibration, growth factor promotion, moisturizing ability, collagen production promotion, and immune-enhancing functions. The chemical structure of Schizophyllan is as follows.

Skirt mushrooms can be applied to the pharmaceutical industry (immune modulators, anti-cancer agents, drug delivery materials, dermatitis/wound treatments, etc.), cosmetics industry (moisturizer, softener, hardener, etc.) or feed industry (immune-enhancing animal feed additives, etc.).

In the present invention, a biological (microbial fermentation) conversion medium capable of producing efficacious components of β-glucan and β-glucan with high efficiency was developed through statistical methods and various combination experiments.

In the case of the finally developed bioconversion process according to an embodiment of the present invention, it showed a beta-glucan productivity of about 20 g/L, which is 2 to 3 times higher than the control conditions in which astronomical dong and soybean embryo are not added, Yp/x, which shows productivity per unit cell, also increased more than 5 times.

Total amino acid content is about 1.5 times that of the same concentration of astronomical extract from Astronomical dong, tyrosine alanine threonine of 16 amino acids is about 1.3 to 1.7 times, leucine phenylalanine (Phenylalanine) glycine ( Glycine) increased by approximately 2.6 to 3 times, especially in the case of Methionine, which was 10 times higher than that of other amino acids, and the total flavonoid content was also noticeably increased.

This increase in amino acid content based on essential amino acids can be said to be the result of confirming the potential as a functional material to help treat specific diseases such as obesity and Alzheimer's disease, as well as improving health conditions such as immunity and hair and skin. .

Therefore, the biological (fermentation) conversion products manufactured according to the present invention can be applied not only to food and cosmetics, but also to quasi-drugs and pharmaceuticals as functional materials.

In order to achieve the above object, in one embodiment of the present invention, glucose 69.6 g/ℓ, astronomical dong dry powder 77 g/ℓ, soybean germ dry powder 1.5 g/ℓ, sodium nitrate 20 g/L, potassium phosphate 15 g/ℓ And it may provide a culture medium for biological (fermentation) conversion containing 2 g / ℓ of magnesium sulfate.

<Example> Liquid Culture Method of Skirt Mushroom Mycelium for Biological (Fermentation) Conversion Process

(1) Preparation stage of raw materials for astronomical dong and soybean embryo

Each of the raw materials that have been washed and cut is prepared by carrying out a drying operation in a hot air dryer capable of mass drying and then pulverizing and pulverizing the raw materials of each natural material.

(2) Cultivation stage of skirt mycelia mycelia for fermentation conversion

After collecting aseptically the mycelium grown using agar medium (potato starch 4g glucose 20g, agar 15g, distilled water 1ℓ) (using mycelium of high-producing beta-glucan strain Schizophyllum commune QG143-1 developed by Kyugen Biotech) The liquid medium (potato starch 4 g, glucose 20 g) was cultured at 28° C. for 6 days. The cultured cells were collected aseptically and inoculated to 10% (v/v) in the growth medium of the same component, stirred at 150 rpm, and shaken and cultured at 28°C for 2 days to be used as seed for the fermentation conversion process.

This culture for biological (fermentation) conversion process was inoculated with 10% (v/v) of seed germs and shake cultured at 28°C for 150 rpm for 4 days.

(3) Development stage of biological (fermentation) conversion process of astronomical dong and soybean embryo

Optimization conditions were determined based on statistical methods by adding astronomical dong and soybean embryos in order to optimize the culture medium of the shiitake mushroom for bio (fermentation) conversion. To this end, the medium factor optimization by the One Factor At a Time (OFAT) method and the Plackett-Burman design and the optimization of the medium concentration by the Full Factorial design (FFD) and Response surface method (RSM) are performed. Did.

In one embodiment of the present invention, through the OFAT (One Factor At a Time) method and the Plackett-Burman design, etc., the remaining source of the culture medium required for the bio (fermentation) conversion process of astronomical dong and soybean embryo (glucose) and Nitrogen sources (sodium nitrate) and inorganic salts (potassium phosphate, magnesium sulfate) were selected considering economics and productivity. Thereafter, through the use of full factorial design (FFD) and response surface method (RSM), which are statistical media optimization methods, the effectiveness of medicinal mushrooms is enhanced while enhancing the effectiveness of astronomical dong/soybean fermentation conversion products using skirt mushrooms. The optimal medium concentration for high production of the component beta-glucan was determined as follows: glucose 69.6 g/l, astronomical dong dry powder 77 g/l, soybean embryo dry powder 1.5 g/l, sodium nitrate 20 g/L, phosphoric acid 15 g/L potassium and 2 g/L magnesium sulfate.

1. Investigation of the composition of the skirt culture medium for the biological (fermentation) conversion process

To optimize the production medium of astronomical dong/soybean embryo fermentation conversion products, 4 carbon sources (Glucose, Maltose, Sucrose, Lactose), 5 nitrogen sources (soybean embryo, Yeast extract, Soytone, astronomical dong, NaNO3), 2 inorganic salts (KH ₂ A medium that positively affects the EPS (beta-glucan productivity), Yp/x, and total flavonoid values through PBD (Plackett-Burman Design) experiments with a total of 12 media components including PO ₄ and MgSO ₄ ) The ingredients were confirmed, and a combination experiment according to the One Factor At a Time (OFAT) method was performed to confirm a suitable media combination for low-cost industrial medium for mass production. Based on these results, the effect of each medium component and the interaction between the mediums were confirmed by using the FFD (Full Factorial Design) method, which is one of the statistical experimental methods, using glucose, an economical low-cost raw material, as a carbon source.

2. Investigation of the ingredients of the production medium of skirt mushroom-Astronomical dong through Full Factorial Design (FFD)

FFD (Full Factorial Design) is a representative experimental method that can confirm the interaction between each factor among statistical media optimization methods, and systematically affects the effect of media composition on beta-glucan productivity and total flavonoid value. Analysis.

FFD is a method of finding the optimal condition through the analysis of a first-order regression model that assumes a linear model of factors and factors and a sub-combination of factors and levels, where n is the number of levels and k is the factor. The number of experiments is determined by (nk). In one embodiment of the present invention, (1) low level and (+1) high level have two levels, soybean embryo, astronomical dong, NaNO ₃ , KH ₂ PO _{4 with} 4 factors (2 ^k )trials (Number of experiments), that is, 16(2 ⁴ )+2(center point) experiments were performed.

The result of ANOVA analysis is a very reliable level with a P value of <0.0001, which means that the experimental result is more than 99.999 accurate (Table 1).

[Table 1]

Looking at the interaction graph (Fig. 1) showing the effect of media components on beta-glucan productivity (measured by EPS value) and alternating factors in the FFD regression analysis results, regardless of the concentration of astronomical dong and soybean embryo, the the higher the concentration of NaNO ₃ NaNO ₃ and the higher the amount of addition of KH ₂ PO _4, and to increase the productivity, the concentration of the soybean embryo cheonmundong schedule was confirmed that the increase in productivity.

Looking at Table 2, combinations 1 to 4 in which both NaNO ₃ and KH ₂ PO ₄ were added at a low level and combinations 13 to 16 in which both NaNO ₃ and KH ₂ PO ₄ were added at a high level were considered as combinations 1 to 4 Silver pH 4.39 ~ 4.52, beta glucan productivity 10.4 ~ 11.75g / L 13 ~ 16 combinations of pH 3.99 ~ 4.23, 17.89 ~ 20.35g / L beta glucan productivity was confirmed that can be seen. Both of NaNO ₃ and KH ₂ PO ₄ are medium components with pH buffering function, and it is thought that the regulation of pH change when adding natural product-derived raw materials, astronomical dong and soybean embryo, affects beta-glucan productivity very closely. This can be said to be in line with the tendency shown in the results of the interaction graph presented above.

[Table 2]

Through these results, it was confirmed that NaNO ₃ and KH ₂ PO ₄ are important factors for correcting the pH decreased by astronomical dong added as a medium component, and accordingly, concentration adjustment and fermentation conversion of NaNO ₃ and KH ₂ PO ₄ Response Surface Method (RSM) was performed to optimize the medium concentration for bio (fermentation) conversion that can increase the active ingredient of the product.

Accordingly, RSM has a beta-glucan productivity (measured by EPS value) of 20.35 g/L and Yp/x value among combinations 13 to 16 in which NaNO ₃ and KH ₂ PO ₄ are added at a high level of 20 g/L. This was performed by setting the combination number 16, which was the highest with 2.84, as the center point.

3. Optimizing the production medium of skirt mushroom-cheonmun-dong through Response Surface Method (RSM)

The Response Surface Method (RSM) is used to find the optimal condition (optimal medium concentration) that represents the maximum response to the change of the dependent variable (EPS productivity) due to the interaction of various experimental factors, that is, the independent variable (medium component). It is a statistical analysis method used. With RSM, it is possible to quickly determine the optimal conditions and to understand the interaction in various conditions. In addition, the optimal point in the part that has not been tested by small-scale experiments has a predictable advantage.

The response surface analysis method analyzes the optimal conditions of the second-order regression model, which assumes a nonlinear model when finding the optimal conditions at the factor level for the response, that is, changes in the dependent variable (EPS productivity) due to the interaction of independent variables (medium components). This is a statistical analysis method used to find the optimal condition (optimal medium concentration) that represents the maximum response value. These RSMs have the advantage of being able to quickly determine the optimal conditions by predicting the optimum point in the non-experimental part as a small-scale experiment, and have the advantage of understanding the interaction in various conditions. It is subdivided into a central composite design method that adds the center point and the experimental point of the axis point to the factor experiment point of the Box-Behnken Design and Factorial Design method.

The researchers have used the response surface experimental design as to make the method easily center the 2 ^K factor experiments (center points) and the central composite design was added to the axis point (axial points) (central composite design ) (CCD). In the central synthesis planning method, the number of center points (n ₀ ) is not limited (four key points are used in this study), and the number of axial points (α) is 2k. Dog axial point experiments were performed. The total number of experiments is 2 ^K +2k+n ₀ =2 ⁴ +2*4+n ₀ , so the experiment was conducted under 28 selected conditions.

The results obtained by the central synthesis planning method are analyzed statistically using the Design-Expert 6.0 program to obtain a regression equation to examine the mutual effects on each medium component, and optimal culture conditions for beta-glucan productivity (measured by EPS value). Was obtained. At this time, the significance for the entire model is determined by the P value given in the ANOVA analysis, and is generally recognized when P<0.05. The optimum culture conditions and the optimum concentration of the biological (fermentation) conversion medium were analyzed using a 3D response surface map and a contour plot at the point where the f value of the second-order polynomial was maximized.

As a result, a significant result with a P value of 0.0001 was obtained, and a second-order polynomial that explained the effects and interactions of each medium component was obtained (Table 3).

[Table 3]

[Table 4]

As a result of the experiment, high productivity was confirmed in combinations 13 to 16 in which NaNO ₃ was added at a high level. Among them, the EPS content of combination 15 was the highest at 25 g/L, and in combination 14 The Yp/x value was as high as 3.97. Overall, the total flavonoid was found to be higher in the medium to which the astronomical sinus was added compared to the control medium to which no astronomical sinus was added.

From the perspective of beta-glucan production (measured by EPS value) through the graph confirmed through the second-order polynomial, soybean embryos and astronomical dongs interact inversely and affect productivity, and KH ₂ PO4 and When the concentration of NaNO ₃ was maintained, productivity increased as the amount of soybean germ added was low. In addition, as in the previous results, it was confirmed that astronomical dong and NaNO ₃ interact very closely with each other and directly affect productivity.

The contour plot of Nano ₃ and NaNO ₃ shows that NaNO ₃ is very sensitive to the concentration of astronomical sinus at concentrations lower than the central point, so the curve interval is very narrow, but at 20 g/L or higher, the interaction with astronomical sinus decreases. It was also found to have a small impact on productivity. Through this, the optimum concentration of NaNO ₃ , which is a nitrogen source and pH buffering, is expected to be between 0 and -1, and the maximum curved surface of the reaction surface map shows a wide pleading from -2 to +2, which increases the concentration of astronomical dong. It is necessary to check the productivity according to the.

Therefore, the interaction and productivity of the medium with increasing astronomical dong were confirmed by experiments of three conditions of NaNO _{3 with} a maximum concentration of 20 g/L and one factor at a time (OFAT) combination experiment with high concentration of astronomical dong.

17, 47, 77, 107, 137 g/L of astronomical dong and 10, 15, and 20 g/L of NaNO ₃ were planned in combination, and the remaining medium components were Glucose 69.6 g/L, KH ₂ PO ₄ 15 g/L, MgSO ₄ 2 g/L It was carried out by fixing with L. As a result, in the trial in which NaNO ₃ was added at 20 g/L, beta-glucan productivity was high, and the total flavonoid content tended to increase as the amount of astronomical copper increased. In particular, the total flavonoid content of the bio (fermentation) conversion product was significantly increased compared to the total flanoid content of the extract before performing the bio (fermentation) conversion process.

[Table 5]

By determining the combination of the 9th, which had a relatively high total flavonoid content of 0.75 among the 6th and 9th, which had the highest productivity of beta-glucan, comparing the productivity in the fermenter with the amino acid content before and after the bio (fermentation) conversion process and antibacterial effects The effectiveness of biological (fermentation) conversion products under optimized conditions was confirmed.

Through this process, the researchers were able to compare and analyze the culture characteristics in the incubator for astronomical dong/soybean embryonic (fermentation) conversion and before and after the process, and confirm the superiority of the development material as a biological (fermentation) conversion product. .

The mycelium of Schizophyllum commune QG143-1, a beta-glucan high-producing strain developed by QGEN Biotech, was grown and cultured in a PDB (Potato Dextrose Broth) medium to perform the process of converting astronomical/soybean embryos (fermentation) in a 5L culture medium After the culture medium for conversion of the biological (fermentation) provided in one embodiment of the present invention (glucose 69.6 g/ℓ, dry powder of astronomical dong 77 g/ℓ, dry powder of soybean germ 1.5 g/ℓ, sodium nitrate 20 g/L, phosphoric acid Inoculated in a bio-incubator containing 15 g/L of potassium and 2 g/L of magnesium sulfate). 3.5L, which is 70% of the bio-cultivator, was used as the operating volume, and maintained at 150 rpm for agitation speed and 1 vvm for gas purging rate, and cultured at 28° C. for 4 days (FIG. 6 ).

As a result, it was possible to confirm the reproducible beta-glucan productivity 2 to 3 times higher than the control condition, the Yp/x value representing the production yield per unit cell was 2.04, and the total flavonoid content was 0.94 g/L, compared to before the culture. It increased by about 36 times. These results of biological (fermentation) conversion products of astronomical dong/soybean embryos were also confirmed in various efficacy evaluation experiments.

In addition, it was confirmed through a disc assay experiment of Escherichia coli and Staphylococcus aureus that it has superior antibacterial effects compared to the conventional extract (hot water extract) and the control group (FIG. 7 ).

As a result of amino acid analysis, the total amino acid content of astronomical dong/soybean embryonic (fermented) conversion products increased by about 1.5 times compared to the existing extracts based on 16 primary amino acids, and tyrosine alanine threonine About 1.3 to 1.7 times, Leucine phenylalanine (Phenylalanine) Glycine (Glycine) increased approximately 2.6 to 3 times. In addition, in the case of Methionine, the increase rate was 10 times higher than other amino acids (Table 6, Table 7, Figure 8). This increase in amino acid content based on essential amino acids can be said to be the result of confirming the potential as a functional material to help treat specific diseases such as obesity and Alzheimer's disease, as well as improving health conditions such as immunity and hair and skin. . Amino acids are not only a raw material for skin and hair, but also play an important role in maintaining moisture in skin and hair. In skin health, amino acids are ① regeneration of the skin and regeneration of the epidermis, ② skin cells, collagen raw materials, and dermis regeneration. In addition, ③ acts as a keratin moisturizing ingredient that protects the skin, increases moisturizing power, and ④ prevents oxidation of the epidermis and dermis through antioxidant action.

[Table 6]

Derivatization reagents and methods for amino acid analysis conditions/amino acid analysis

[Table 7]

Analysis of amino acid content of amino acid standards and experimental samples

4. Evaluation of cleaning power of fine first

The dust is classified into TSP (Total Suspended Particles), which is 50㎛ or less, and Particulate Matter (PM), which has a very small particle size. The fine dust is further divided into fine dust (PM10) having a diameter less than 10 μm and fine dust (PM2.5) having a diameter less than 2.5 μm. If PM10 is about 1/5 to 1/7 smaller than a human hair diameter (50 to 70 µm), PM2.5 is very small, about 1/20 to 1/30 of the hair. As such, the fine dust stays in the air because it is so small that it is invisible to the eye, and may penetrate the lungs, etc. through the respiratory tract, or move into the body along the blood vessels, thereby adversely affecting health. The World Health Organization (WHO) has been providing air quality guidelines for fine dust (PM10, PM2.5) since 1987, and in 2013 the International Agency for Research on Cancer (IARC) under the World Health Organization. In the study, fine dust was designated as a group 1 carcinogen (Group 1) where carcinogenesis was confirmed in humans. The components that make up the fine dust may vary depending on the region, season, weather conditions, etc. where the fine dust has occurred. In general, it is composed of lumps (sulfate, nitrate, etc.) formed by the reaction of air pollutants in the air, and carbon generated from the process of burning fossil fuels such as coal and petroleum, and minerals generated from soot and ground dust.

The fine dust cleaning power of the cosmetic composition (Table 8, body wash composition) using bioconversion products of astronomical dong and soybean embryo obtained from the medium having the optimum concentration identified above was tested. After applying a fine dust substitute to the forearm of 20 subjects, it was performed by a method of cleaning using test samples and lukewarm water, and analyzed using Folliscope and Image-Pro Plus.

1) Cosmetic composition using bioconversion products of astronomical dong and soybean embryo

[Table 8]

Body wash cosmetic composition

2) Substitute for fine dust (super fine dust mimic and fine dust adsorption mimetic)

① Superfine dust mimetic component name: Carbon Black

-Manufacturer: Sensient Cosmetic Technologies (Batch C3950)

-INCI name: CI77266

-Cas number: 1333-86-4

-Average particle size: 0.22㎛

② Fine dust adsorption mimetic

: Carbon black with a particle diameter of 2.5 µm or less is stored in a dessicator, and carbon black and distilled water are mixed at a ratio of 1: 10 immediately before the test to prepare a fine dust adsorption material and used for testing.

3) Control group (standard sample): Ivory original soap (All ingredients list: fragrance, tetrasodium ID, glycerin, palm kernel acid, palm acid, sodium palm kernelate, sodium palmate, sodium tallowate, tallow acid , Sodium cocoate, coconut acid, purified water, sodium chloride)

4) Test order

-The subject washes the forearm on both sides with a standard cleaning agent, taps lightly with a paper towel, removes moisture, and is stable under constant temperature and humidity conditions (20 to 24°C, 40 to 60%RH) for 30 minutes.

-After dividing the 2cm X 2cm square test area on both forearms of the subject, designate them as the test site (washed with cosmetic composition using bioconversion products of astronomical sinus and soybean embryos), control site (soap wash), and water wash, respectively do.

-Using Folliscope and DSLR, take images of each part before applying fine dust substitute.

-After applying a quantity (20 µl) of fine dust substitute to each site, wait for 30 minutes to dry.

-Using Folliscope and DSLR, after applying fine dust substitute, take an image of each part.

-Each test site is rubbed about 20 times using test sample, control sample foam and water.

-After washing each part with running water for 10 seconds, wait for 20 minutes to dry.

-After cleaning using Folliscope and DSLR, images of each part are taken.

5) Evaluation method

① Efficacy evaluation of fine dust cleaning power using Folliscope and Image-Pro Plus

-After application and after cleaning, the adsorption amount of fine dust substitute material (△) is calculated using the pixel value analyzed by Image-Pro Plus as follows, and the amount of fine dust substitute material adsorption after cleaning of the test site and the control area (△ ) Verify the statistical significance of the differences.

Adsorption amount of fine dust substitute after application (△) = Residual area of fine dust substitute after application (Pixel)-Residual area of fine dust substitute before application (Pixel)

Adsorption amount of fine dust substitute after cleaning (△) = Residual area of fine dust substitute after cleaning (Pixel)-Residual area of fine dust substitute before application (Pixel)

-Calculate the percentage value of the cleaning effect of the sample using the following formula and verify the statistical significance of the difference (%) difference in improvement between the test site and the control site (soap).

② Safety evaluation by dermatologist

-Evaluate the occurrence of side effects (erythema, edema, swelling, itching, burning sensation, stiffness, tingling, and other abnormalities) caused by the use of the product during the test period.

6) Test result

[Table 9]

Fine dust cleaning power test result

(Test site: cleansing the cosmetic composition using bioconversion products of astronomical sinus and soybean embryo, control site: general soap cleaning)

As a result of washing the test site coated with the fine dust substitute material using the body wash cosmetic composition for 20 subjects, the adsorption amount of fine dust substitute material (△, Pixel) compared to the control site cleaned with soap (control sample) ) Is used as a statistically significant level (p<0.05), and the improvement rate (%) is 97.50% and 93.79%, respectively, in the test site and the control area, using test samples (cosmetic composition using bioconversion products of astronomy sinus and soybean embryos). It was confirmed that the test site washed with a statistically significant level (p<0.05) is higher than the control site cleaned using a control sample, and this cosmetic composition has excellent cleaning power (cleansing effect) against fine dust. Is determined (Table 9 and Figure 9). In addition, no adverse events were observed in all subjects during the test period, so it is considered a safe sample.

5. Evaluation of keratin improvement efficacy

The epidermis of human skin consists of several layers of keratinocytes that migrate to the superficial layer as they differentiate from the stratum basalis. As the keratinocytes finally differentiate, they lose their viability and are converted into corneocytes. The outermost layer of the epidermis formed by these dead cells is called the stratum corneum. The lower stratum corneum has a stronger binding capacity between keratinocytes than the upper stratum corneum and acts as an important barrier to prevent skin from penetrating foreign substances, protects skin from various physical forces, and also has antimicrobial peptides, causing infection by contaminating bacteria. It also acts to prevent. Since the keratinocytes constantly differentiate and flow into the stratum corneum, the cells of the upper stratum corneum are loosened from each other and easily detached from the skin due to weak external stimuli to maintain homeostasis of the skin. (desquamation) is a process and free keratinocytes appear clinically in the form of scale or commonly called keratin. Important mechanisms in the process of exfoliation of keratinocytes are as follows. First, there is a decomposition process of corneodesmosome that mediates binding between keratinocytes, which is mainly achieved by various proteolytic enzymes whose activity is controlled by pH in the stratum corneum. Second, the process of disintegration and decomposition of lipids between the stratum corneum is also closely related to the exfoliation process of keratinocytes, which is also related to skin barrier damage associated with the use of detergents. Lastly, in the process of hydration of the skin, there is a mechanism of exfoliation of the epidermis that occurs in the process of changing the surface area of the keratinocytes by volume, and it appears that the keratin is pushed away.

In this experiment, the effect of immediate keratin improvement of a cosmetic composition (Table 8, body wash composition) using bioconversion products of astronomical sinus and soybean embryo obtained from the medium having the optimum concentration identified above was tested. After using the test sample (cosmetic composition of Table 8) on the face of 21 subjects, it was analyzed using Corneofix F20 (Courage & Khazaka, Germany) and Visioscan VC98 (Courage & Khazaka, Germany).

1) Test order

-After washing using a standard cleaning agent, the subject was gently knocked with a paper towel to remove moisture, and then stabilized under constant temperature and humidity conditions (20 to 24°C, 40 to 60% PH) for 30 minutes.

-Designate the test site as follows so that the measurement can be made at the same site for each evaluation. → Test site: center of the forearm 15 cm from the wrist.

-Using Corneofix and Visiocan, measure the desquamation index before using the sample at the test site.

-Use the test sample once on the test site.

-Using Corneofix and Visiocan, measure the keratinization index at the same site in the same way as before use.

2) Evaluation and analysis method

① Evaluation of keratin improvement efficacy using Visioscan

-Before and after the test, the desquamation index is measured mechanically.

-The degree of improvement is derived as a% value as follows,

② Safety evaluation by dermatologist

-Evaluate the occurrence of side effects (redness, edema, swelling, itching, pain, burning sensation, stiffness, tingling, and other abnormalities) caused by the use of samples during the test period.

3) Test result

Table 10

Degree of improvement of keratin index (Desquamation index, %)

Significant probability : *p<0.05, **p<0.01, ***p<0.001

The improvement rate of the desquamation index of the test site measured using Corneofix and Visioscan was found to be 18.96%. This is a statistically significant level (p<0.05) after use of the test sample, a decrease in the keratinization index (Desquamation index), it is determined that the test sample has an immediate keratin improvement effect of the skin (Fig. 10). In addition, no adverse skin reactions were observed from all subjects during the test period, which is considered to be a safe sample.

6. Evaluation of skin moisture content improvement efficacy

Human skin is the primary barrier to protect the human body from various stimuli originating from the external environment. It is also a protective barrier that prevents moisture from the body, which is absolutely necessary to maintain bioactivity, from escaping to the outside. The human body is inevitably exposed to a relatively dry environment compared to the skin most of the time, and because of this, the skin, especially the stratum corneum, has various substances that store moisture and prevent evaporation, which plays a very important role in maintaining moisture in the body. The two most important factors for the moisturizing function of the stratum corneum are the intercellular lamellar lipids, which act as a film to prevent evaporation of moisture, and as another factor, the decomposition product of the filarggrin in the keratinocytes. There is a natural moisturizing factor composed mainly of ole, which functions to trap moisture in the stratum corneum. In various skin diseases or aged skin, the function of these two main factors is deteriorated and the skin's moisturizing condition deteriorates and dryness can easily occur. In this case, the moisturizer is a very important treatment to relieve pruritus or other skin abnormalities caused by dryness. It can be a means. In addition, the moisture in the stratum corneum can help maintain the skin barrier in a healthy state by helping maintain the activity of enzymes with various physiological activities, so the moisturizer serves to keep the skin of normal people healthy as well as dry skin. can do.

In this experiment, the sample through the improvement of the moisture content of the test site before and after the use of the cosmetic composition (Table 8, body wash composition, test substance) using bioconversion products of astronomical dong and soybean embryo obtained from the medium having the optimum concentration identified above The immediate skin moisture content improvement efficacy indicated by was tested. After using the test sample (cosmetic composition of Table 8) in the forearm of 22 subjects, it was analyzed using Corneometer CM 825 (Courage & Khazaka, Germany).

1) Test order

-After washing with a standard cleaner, tap lightly with a paper towel to remove moisture, and then stabilize under constant temperature and humidity conditions (20~24℃, 40~60%RH) for 30 minutes.

-For each evaluation, segment the measurement site and measure the device so that the measurement can be made at the same site.

-After measuring corneometer 5 times each, record the average of the measured values as the measured value.

-After using a test sample of 2mg/cm ^{2 at} the test site (forearm) and waiting for 30 minutes under constant temperature and humidity conditions (20~24℃, 40~60%RH), evaluate the device at the same site in the same way as before using the sample. Enforce.

-The dermatologist checks for abnormal reactions (irritation symptoms such as pruritus and erythema).

2) Evaluation method

① Evaluate the effect of improving moisture content using corneometer

-Before and after using the sample at the test site, measure the change in skin moisture content using a corneometer, and check the improvement rate as follows.

② Safety evaluation by dermatologist

-Evaluate the occurrence of side effects (redness, edema, swelling, itching, pain, burning sensation, stiffness, tingling, and other abnormalities) caused by the use of samples during the test period.

3) Test result

[Table 11]

Evaluation result of moisture content of stratum corneum

Significant probability : *p<0.05, **p<0.01, ***p<0.001

Corneometer measurement value (A.U.) indicating skin moisture content increased by 10.72% after use compared to before sample use, indicating that the moisture content of the stratum corneum at the test site was improved. The test site where the test sample was used once is statistically significant (p<0.05) higher than before use, so the moisture content of the stratum corneum layer is increased, so it is judged that the test sample has an immediate skin moisture content improvement effect. Also, no adverse reactions were observed in all subjects, so it is considered a safe sample.

Statistical analysis method

1) Check the significance using the Minitab 19 (Minitab2 19.2, Minitab Inc.) program.

-If the result is estimated to be a normal distribution through the normal-test (Ryan-Joiner Normality Test), check the significance through the parametric statistical method below.

-Comparison of the results before and after within the same group: The comparison of the measured values before and after the test uses a paired t-test. When repeated measurements are repeated three or more times, the significance level p <through repeated measurement ANOVA. Check the significance at the 0.05 level.

-Comparison of results between two or more different groups: Comparison of results between two groups uses Welch's t-test, and comparison of results between groups of three or more uses significance level p <0.05 through one-way ANOVA. Check the significance at the level.

-Comparison of the results of two or more different groups measured repeatedly: Repeated measure ANOVA confirms the significance at the significance level p <0.05, and the initial measurement values are significantly different between groups. The difference in results between groups is checked through analysis of covariance using covariates.

-When the normality is rejected in the Ryan-Joiner Normality Test, the significance is confirmed through the nonparametric statistical method below.

-Comparison of the results before and after within the same group: Wilcoxon signed rank test is used to compare the measured values before and after the test. When repeated measurements are repeated three or more times, the significance is checked at the significance level p <0.05 through the Friedman test. .

-Comparison of result values between two or more different groups: Mann-Whitney U test is used to compare the results between two groups, and comparison of result values between three or more groups is significant at the significance level p <0.05 through the Kruskal-Wallis test. To confirm.

-Comparison of the results of two or more different groups measured repeatedly: The Friedman test is used to check the difference in results between groups at the significance level p <0.05.

-All data are summarized as mean and standard deviation for continuous variables and frequency and percentage for categorical variables.

<Production Example>

Hereinafter, an example of manufacturing a cosmetic product containing the bioconversion product according to the present invention as an active ingredient will be described, but the present invention is not intended to limit it, but only to be specifically described.

1. Manufacture of body wash

-Body wash that helps to clean fine dust, improve keratin and moisturize

2. Manufacture of cleansing water (product for lightly acidic makeup (pH 6.0) and light makeup and waste products)

-Colorless and transparent liquid cleansing water containing moisturizing and skin care ingredients and appropriate ingredients to remove makeup and wastes

-After removing makeup and waste materials lightly and without stickiness, the skin is moist and shiny.

3. Preparation of weakly acidic foam cleanser

4. Preparation of cleanser

Claims (12)

As a cosmetic composition for cleaning the skin containing the bio-converted product obtained by inoculating and cultivating skirt mushrooms in a culture medium containing astronomical powder and soybean embryo powder as an active ingredient,
The culture medium is 65 to 75 parts by weight of glucose, 72 to 82 parts by weight of astronomical dong powder, 0.5 to 5 parts by weight of soybean embryo powder, 15 to 25 parts by weight of sodium nitrate, 10 to 20 parts by weight of potassium phosphate and 1 to 7 parts by weight of magnesium sulfate Includes wealth,
The bioconversion product cosmetic composition for cleaning the skin, characterized in that it comprises beta-glucan, amino acids and flavonoids.
delete The method according to claim 1,
The skirt mushroom is a cosmetic composition, characterized in that cultured in a liquid medium.
The method according to claim 1,
The skirt mushroom is a cosmetic composition, characterized in that obtained by incubating for 5 to 7 days in a liquid medium for stationary, and shaking culture for 1 to 3 days.
delete delete The method according to claim 1,
The culture is a cosmetic composition characterized in that 5 ~ 15% (v / v) of the shiitake mushroom was inoculated.
delete delete As a cosmetic composition for cleaning fine dust containing the bio-conversion product obtained by inoculating the skirt mushrooms in a culture medium containing astronomical dong powder and soybean embryo powder as an active ingredient,
The culture medium is 65 to 75 parts by weight of glucose, 72 to 82 parts by weight of astronomical dong powder, 0.5 to 5 parts by weight of soybean embryo powder, 15 to 25 parts by weight of sodium nitrate, 10 to 20 parts by weight of potassium phosphate and 1 to 7 parts by weight of magnesium sulfate Includes wealth,
The bioconversion product cosmetic composition for cleaning fine dust, characterized in that it comprises beta-glucan, amino acids and flavonoids.
As a cosmetic composition for keratin improvement comprising a bio-converted product obtained by inoculating a skirt mushroom in a culture medium containing astronomical dong powder and soybean embryo powder as an active ingredient,
The culture medium is 65 to 75 parts by weight of glucose, 72 to 82 parts by weight of astronomical dong powder, 0.5 to 5 parts by weight of soybean embryo powder, 15 to 25 parts by weight of sodium nitrate, 10 to 20 parts by weight of potassium phosphate and 1 to 7 parts by weight of magnesium sulfate Includes wealth,
The bioconversion product cosmetic composition for improving keratin, comprising beta-glucan, amino acid, and flavonoid.
As a cosmetic composition for improving the moisture content of the skin containing the bio-converted product obtained by inoculating the skirt mushrooms and incubating the culture medium containing astronomical powder and soybean embryo powder as an active ingredient,
The culture medium is 65 to 75 parts by weight of glucose, 72 to 82 parts by weight of astronomical dong powder, 0.5 to 5 parts by weight of soybean embryo powder, 15 to 25 parts by weight of sodium nitrate, 10 to 20 parts by weight of potassium phosphate and 1 to 7 parts by weight of magnesium sulfate Includes wealth,
The bioconversion product cosmetic composition for improving skin moisture content, comprising beta-glucan, amino acids and flavonoids.

Images