KR102060237B1 - A cosmetic composition comprising fermented dendrobium candidum and method for preparing the same - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising the fermentation of Dendrobium candidum fermented with Bacillus bacteria or lactic acid bacteria, having a skin wrinkle improvement and whitening effect, and a method for preparing the same, the composition of the present invention promotes collagen secretion And inhibitory effect on pigment production.

Description

Cosmetic composition comprising iron corrugated fermented product and preparation method thereof {A COSMETIC COMPOSITION COMPRISING FERMENTED DENDROBIUM CANDIDUM AND METHOD FOR PREPARING THE SAME}

The present invention relates to iron corrugated grains fermented with Bacillus or lactic acid bacteria, cosmetic composition comprising the same, and a method for preparing such compositions, compared to powders or extracts of iron corrugated grains and fertilized grains fermented with Bacillus or lactic acid bacteria skin wrinkles and Characterized by the improvement of whitening efficacy.

In the East, there are old feeds for preventing, preventing and treating diseases from various plants. Iron bark grain is also known as one of various stone grains. With the recent development of analytical techniques, the potency components of the plant are known, and researches are being actively conducted. However, most of these plant-derived natural products are often present as unactivated forms in the case of polymers bound to polysaccharides. In general, the various ways to take advantage of the body metabolism is taking advantage of the situation.

Dendrobium candidum (鐵皮 石斛) is a monocotyledonous plant belonging to the orchid family Orchidaceae, an evergreen perennial grass that grows on rocks or dead tree trunks, and has a lot of thick roots from its roots. Iron corrugated grains are mainly distributed in China and Korea, and it is reported that polyphenols and dendrocandins in the form of polysaccharides and glycols are contained. Also, dendrocandines C, D and E are known as indicators or active substances. In Chinese and Korean medical texts, it is said to be effective in eliminating or lowering the heat in the stomach, producing essences and quenching thirst, strengthening the musculature, and helping with diabetes, night blindness, and high coordination. Iron corrugated grain was an expensive herb before plant molecule biotechnology became active, but recently, it is produced through cell culture.

Skin is also known as a second body organ, and in the most common case, it is common for foods to be consumed by humans to decompose, synthesize, and deliver blood vessels through the blood vessels. Accordingly, in the case of cosmetics directly applied to the skin, fermented products of various materials have recently been developed under the assumption that a substance that is decomposed and synthesized by microorganisms in the human body will be more effective.

Thus, in the present invention, the decomposition and change by the digestive metabolism of conventionally known cortical granules was carried out by culturing under artificial conditions of Bacillus bacteria or lactic acid bacteria derived from the intestine, and attempted to obtain the maximum skin effect of the cortical granules. The fermentation by Bacillus or lactic acid bacteria to produce or decompose into a more active active material to develop a cosmetic composition effective for skin health to complete the present invention.

It is an object of the present invention to provide a cosmetic composition and a method for producing the same, which exhibits skin wrinkle improvement and whitening efficacy through fermentation of Bacillus or lactobacillus of Cortex.

The present invention aims to search for a skin maximizing composition of fermented grain fermentation and to secure a process capable of mass production.

The composition according to the present invention promotes collagen secretion and has an inhibitory effect on the production of pigments, thus showing an excellent effect on skin wrinkle improvement and whitening.

1 is a block diagram showing a method for producing Bacillus bacteria or lactic acid bacteria fermentation containing 5% iron cortex grains in accordance with the present invention.
FIG. 2 shows Bacillus caldolyticus, Bacillus subtilis, Bacillus coagulans, Bacillus laevolactic according to the control group (untreated group), and Example 1 ), L. pylori fermentation fermented with Lactobacillus acidophilus, Bifidobacterium infantis, Streptococcus thermophiles and Bifidobacterium longum, respectively. Collagen secretion (compared to control) in the treated group treated with human-derived fibroblasts by 5%.
Figure 3 is the control group (untreated group), and collagen secretion in the treated group treated with human dermal fibroblasts 1%, 5% and 10% fermented with Bacillus subtilis according to Example 2 (control) Contrast).
FIG. 4 shows Bacillus caldorticus, Bacillus subtilis, Bacillus coagulans, Bacillus rabolactic, Lactobacillus acidphylis, containing 5% iron cortex as substrate as a control (untreated group), and Example 3; , 5% of fermented products fermented with Bifidobacterium inpentis, Streptococcus thermophilis, and Bifidobacterium longgum, respectively, to rat-derived pigment cells to show the inhibitory activity of pigment formation (compared to control). .
Figure 5 is a control group (untreated group), and 1%, 5% and 10% fermented barley grain fermentation fermented with Bacillus coagulans according to Example 4 treated group in the treatment group treated with rat-derived pigment cells (Compared to the control).
FIG. 6 is a photograph showing the improvement of wrinkles and skin tone in the treatment group treated with the formulation containing about 10% of the cortical grain fermentation fermented with Bacillus subtilis and Bacillus coagulans according to Example 5. FIG. It is shown.

EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated in detail.

The present invention provides a cortical granules cosmetic composition fermented with Bacillus bacteria or lactic acid bacteria. The present invention also provides a method of preparing such a composition.

Specifically, the composition according to the present invention comprises iron corrugated fermentation 0.01 to 50% by weight, based on the total weight of the composition.

The method for producing a composition according to the present invention comprises (1) preparing dry corrugated granules, (2) pulverizing the dry matter, (3) culturing Bacillus or lactic acid bacteria prepared in advance, and (4) powdering The iron corrugated grains were put into a Bacillus or lactic acid bacteria culture medium containing Bacillus or lactic acid bacteria, and then (5) the cultures were cultured for at least 48 hours, and (6) the culture solution was filter paper, 0.45 μm and 0.2 μm size filters. Sequential filtration, followed by (7) securing the thus filtered stock solution and preservative treatment, thereby obtaining a composition which ensures the content of the indicator substance in the active form.

In one embodiment, the Bacillus or lactic acid bacteria in the above (3) and (4) step is isolated in the human intestine. In another embodiment, the fermentation conditions of Bacillus or lactic acid bacteria comprises 20 to 35 ℃ and general Bacillus or lactic acid bacteria fermentation medium.

Hereinafter, the method for obtaining the composition of the present invention will be described in detail in the following Examples. The following examples are merely to illustrate the invention, the present invention can be implemented in a number of different forms and of course the content of the present invention is not limited by the following examples.

The test was carried out as in Example 1 below to produce iron barley grain fermentation of the present invention, Bacillus caldorticus, Bacillus subtilis, Bacillus coagulans, Bacillus rabolactic, Lactobacillus acidphyllis, Bifidobacte Leeum Iron cortex was fermented using Inpentis, Streptococcus thermophilis, and Bifidobacterium longgum, and the fermented product was treated with human-derived fibroblasts. It was confirmed that it promotes secretion, and in particular, showed the maximum effect in Bacillus subtilis, it was confirmed that the maximum collagen secretion effect in the test mixed with fermentation products of each Bacillus or lactic acid bacteria.

In Example 2, the Bacillus subtilis fermented product, which showed the maximum effect in Example 1, was treated to human-derived fibroblasts at concentrations of 1%, 5%, and 10%. It was confirmed to promote.

In addition, as in Example 3, Bacillus caldorticus, Bacillus subtilis, Bacillus coagulans, Bacillus rabolactic, Lactobacillus acidphylis, Treatment with fermentation with Bifidobacterium inpentis, Streptococcus thermophilis, and Bifidobacterium longgum showed significant inhibitory effects on hormone-induced plastids, especially Bacillus coagulans Showed the maximum effect.

In Example 4, the Bacillus coagulans fermented product showing the maximum effect in Example 3 was treated with 1%, 5%, and 10% concentrations in mouse-derived pigment cells, and it was confirmed that all of them had inhibitory effect on the production of the pigment.

In addition, as in Example 5, as a result of human testing with a formulation containing about 10% of the cortical grain fermentation fermented with Bacillus subtilis and Bacillus coagulans, fine wrinkles were improved and skin tone was brightened.

Example 1

Human-derived fibroblasts were cultured and the amount of collagen secreted from the cortical granules fermentation using each Bacillus or lactic acid bacteria was measured.

As a basic culture method proposed in the art, after culturing Bacillus or lactic acid bacteria for 24 hours to produce a Bacillus or lactic acid bacteria fermentation product containing 5% iron cortex as a substrate as shown in Figure 1, each fermented product 5% The results of comparing collagen secretion treated to human-derived fibroblasts with the control group are shown in Table 1 and FIG. 2 below.

Strain name Collagen Secretion Rate (%) Control 100 Bacillus caldorticus
(Bacillus caldolyticus) 94.2 Bacillus subtilis
(Bacillus subtilis) 201.0 Bacillus coagullans
(Bacillus coagulans) 101.3 Bacillus Rabolactic
(Bacillus laevolactic) 97.1 Lactobacillus acidphilis
(Lactobacillus acidophilus) 135.0 Bifidobacterium Inpentis
(Bifidobacterium infantis) 153.6 Slip Litococcus Thermophilis
(Streptococcus thermophiles) 122.8 Bifidobacterium longgum
(Bifidobacterium longum) 173.5

Example 2

Human-derived fibroblasts were cultured and the amount of collagen secreted from the cortical granule fermentation using Bacillus subtilis was measured.

Fermented products using Bacillus subtilis were produced in the same manner as in Example 1, and collagen secretion in human-derived fibroblasts treated at concentrations of 1%, 5% and 10% was compared with the control group. And FIG. 3.

Collagen Secretion Rate (%) Control 100 Fermentation product concentration One% 5% 10% Bacillus subtilis
(Bacillus subtilis) 151.6 194.3 215.8

Example 3

Rat-derived pigment cells were cultured, and the effect of inhibiting the pigment production of iron cortical grain fermentation using each Bacillus or lactic acid bacteria was measured.

After culturing each Bacillus bacteria or lactic acid bacteria for 24 hours by the basic culture method proposed in the art to produce a Bacillus or lactic acid bacteria fermentation product containing 5% iron cortex as a substrate as shown in Fig. 5% treatment was performed to determine the effect of inhibiting the production of the pigment. In the cell test, the chromosomes were induced through hormonal treatment, and the inhibition of chromosomal production compared to the control is shown in Table 3 and FIG. 4 below.

Strain name Inhibition degree of pigment formation (%) Bacillus caldorticus
(Bacillus caldolyticus) 25 Bacillus subtilis
(Bacillus subtilis) 37 Bacillus coagullans
(Bacillus coagulans) 44 Bacillus Rabolactic
(Bacillus laevolactic) 15 Lactobacillus acidphilis
(Lactobacillus acidophilus) 16 Bifidobacterium Inpentis
(Bifidobacterium infantis) 25 Slip Litococcus Thermophilis
(Streptococcus thermophiles) 28 Bifidobacterium longgum
(Bifidobacterium longum) 30

Example 4

Rat-derived pigment cells were cultured, and the inhibitory effect on the pigment production of the cortical granule fermentation using Bacillus coagulans was measured.

Fermentation products using Bacillus coagulans were produced in the same manner as in Example 3, and the inhibition of the pigment production of the mouse-derived pigment cells treated at concentrations of 1%, 5% and 10% is shown in Tables 4 and 5 below. .

Inhibition degree of pigment formation (%) Fermentation product concentration One% 5% 10% Bacillus coagullans
(Bacillus coagulans) 36 45 44

Example 5

For each of the five male and female volunteers, a formulation containing approximately 10% of the cortical granules fermented with Bacillus subtilis and Bacillus coagulans was used twice daily for four weeks. Fine wrinkles and skin tone were measured. The measured value was obtained by using the Janus facial diagnosis system device after 4 weeks as fine wrinkles and skin tone as a percentage of the area. The improvement rates before and after using the five formulations are shown in Table 5 and FIG. 6 below as average values.

process male female Fine wrinkles Treatment group (%) 14 8 Skin tone improvement Treatment group (%) 16 9

Based on the results of Examples 1 to 5 above, the composition containing the fermented cortical grain fermented with Bacillus or lactic acid bacteria of the present invention can be used as a material for effective wrinkle improvement and whitening cosmetics.

The above description is merely illustrative of the technical idea of the present invention, and those skilled in the art may make various modifications and changes without departing from the essential characteristics of the present invention. In addition, the embodiments disclosed in the present invention are not intended to limit the technical idea of the present invention but to describe the present invention, and the scope of the technical idea of the present invention is not limited by these embodiments. The scope of protection of the present invention should be interpreted by the following claims, and all technical ideas within the scope equivalent thereto should be construed as being included in the scope of the present invention.

Claims (4)

As a method of producing a cosmetic composition for improving skin wrinkles and whitening, the production method
(1) prepare the dry corrugated granules,
(2) powdering the dried product,
(3) culturing Bacillus subtilis, Bacillus coagulans or a combination thereof prepared in advance to prepare a culture solution,
(4) the powdered iron corrugated grains were added to the culture solution to prepare a corrugated grain mixed culture solution,
(5) incubating the iron corrugated mixed culture solution for at least 48 hours at a temperature of 20 to 35 ℃,
(6) filtering the cultured iron corrugated mixed culture solution,
(7) comprising the step of securing the thus-prepared stock solution, antiseptic treatment. The method according to claim 1, wherein the Bacillus subtilis or Bacillus coagulans in step (3) is isolated in the human intestine. According to claim 1, Bacillus caldolyticus (Bacillus caldolyticus), Bacillus laevolactic (Bacillus laevolactic), Lactobacillus acidphillus (Labtobacillus acidophilus), Bifidobacterium infantis (Bifidobacterium infantis), The method of claim 1, wherein at least one selected from Streptococcus thermophiles and Bifidobacterium longum is added. A cosmetic composition for improving skin wrinkles and whitening prepared by the method according to any one of claims 1 to 3.

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