KR102033260B1 - Food composition for improving fine dust stimulated respiratory diseases with seaweed ear extract and process for preparing the same - Google Patents

Abstract

The present invention relates to a food composition for alleviating fine dust-stimulated respiratory diseases including Undaria pinnatifida Sporophyll and a method for preparing the same and, more specifically, to a food composition for alleviating fine dust-stimulated respiratory diseases including Undaria pinnatifida Sporophyll, which has doubled functionality and palatability of products by processing Undaria pinnatifida Sporophyll, immersing the processed Undaria pinnatifida Sporophyll in a mixed vegetable extract, and aging the same, and a method for preparing the same. According to the present invention, functionality and palatability for alleviating respiratory diseases caused by fine dust are improved by processing Undaria pinnatifida Sporophyll, immersing the processed Undaria pinnatifida Sporophyll in a mixed vegetable extract and aging the same, and thus the food composition of the present invention can be useful for functional food for preventing or alleviating the respiratory diseases caused by fine dust.

Description

Food composition for improving fine dust stimulated respiratory diseases with seaweed ear extract and process for preparing the same}

The present invention relates to foods for improving fine dust stimulation respiratory disease, including seaweed ears, and more particularly, a method for producing and processing the seaweed ears and then immersing and ripening them in a mixed plant extract, the functionality and palatability of the product The present invention relates to a food for improving fine dust-stimulated respiratory disease and a method for producing the same, including seaweed ears having this doubled property.

In Korea, the environment has been continuously deteriorated due to rapid industrialization, industrialization and urban concentration over the last few decades. In particular, environmental pollution in large cities is serious. Due to environmental policy enhanced regulation contaminants such as supply-up of the use of natural fuel SO _2, CO, floating dust concentration, but is gradually decreased, due to the rapid increase in the car fine dust, ozone, nitrogen oxides, hydrocarbons water pollutant Rather, it is increasing.

In addition, China's dependence on coal is more than 70%, so the smog phenomenon continues to increase, and fine dust is expected to occur due to the combination of yellow dust, smog, and high-density dust from Mongolia.

Fine particles are substances suspended in the atmosphere having very complex components such as sulfur oxides, nitrogen oxides, lead, ozone, and carbon monoxide, and are known to be mostly generated from automobile exhaust gas and road dust.

The size and composition of fine dust are very complex and diverse, PM10 is called fine dust with particle size (aerodynamic diameter) of 10㎛ or less, PM2.5 is called ultra fine dust with particle size of 2.5㎛ or less, and PM1.0 is particle size 1.0 It is a fine dust of less than or equal to μm, and the particle size, surface area, and chemical composition are known to determine health effects.

Respiratory effects of fine dust are mainly caused by inflammatory reactions in the bronchioles, such action is to cause or worsen asthma, chronic bronchitis, airway obstruction.

In addition, fine dust may cause respiratory infections by interfering with the inactivation or removal of bacteria in lung tissue. Recently, fine dust is an important risk factor for cardiovascular diseases such as myocardial infarction, stroke, heart rate abnormality and sudden death. It is accepted.

Exposure to fine dust is associated with increased mortality as well as incidence of respiratory and cardiovascular diseases. By age group, the effects of fine dust and yellow dust in preschool children with physically weak immunity and elderly people over 70 This turned out to be bigger.

Respiratory diseases caused by fine dust include cold, bronchitis, pneumonia, tuberculosis, lung cancer and chronic bronchitis. A common cold is a runny nose and stuffy nose in all age groups. Bronchitis is a viral bronchitis that spreads to the lower airway when a viral infection occurs in the upper respiratory tract. Tuberculosis is an infectious disease caused by the infection of Mycobacterium tuberculosis. Chronic bronchitis refers to a disease in which the bronchial mucosa becomes narrow due to fibrotic inflammatory reactions due to the inflammation of the capillary bronchus and the secretion of mucus from the mucosal cells increases, causing airway obstruction.

Therefore, there is a need to prevent and ameliorate diseases caused by such fine dust by providing functional foods and the like using the beneficial ingredients of natural products.

Korea Patent Registration No. 10-1466316 (November 21, 2014) Korean Patent Registration No. 10-1516057 (April 22, 2015)

The present inventors have diligently researched to develop useful products by searching for components effective for fine dust using natural products. As a result, the food produced by processing seaweed ear as described below and then immersing and ripening them in a mixed plant extract is fine. The present invention has been accomplished by discovering that it can be usefully used for patients or consumers who need it by improving the functionality and palatability of improving respiratory diseases caused by dust.

Therefore, an object of the present invention, in one aspect,

Foods for improving fine dust stimulation respiratory diseases, including seaweed ears with improved functionality and palatability to improve respiratory diseases caused by fine dust by processing the seaweed ears and then immersing and ripening them in a mixed plant extract; To provide.

The object of the present invention as described above

a) pretreatment of the collected seaweed;

b) freezing the pretreated seaweed to −30 to −40 ° C .;

c) thawing by immersing the frozen seaweed in wash water;

d) spraying and washing air into seaweed at the same time as the thawing;

e) screening seaweed ears by removing the washing water and foreign matter contained therein;

f) supplying the wash water to seaweed ears to perform the second washing;

g) cutting the washed seaweed into a certain size;

h) blanching the cut seaweed;

i) cooling the poached seaweed slice;

j) fermenting and aging by immersing the cooled seaweed slice in a complex plant extract;

k) packaging the seaweed and complex plant extracts which have been matured into a unit of product; And

l) sterilizing the packaged product;

The complex plant extract is to be achieved by a method for producing a food for the improvement of fine dust stimulation respiratory disease, characterized in that it is produced by extracting a mixed plant comprising seaweed, shiitake mushroom or millilis cordyceps sinensis, gilyeong, windproof and cypress Can be.

According to the present invention for improving the fine dust stimulation respiratory disease and a method for producing the same, the processing and processing of seaweed ears and then immersing and ripening them in a mixed plant extract to improve the functionality and palatability of the respiratory disease caused by fine dust It can be usefully used as a functional food to improve or prevent it.

1 is a manufacturing process diagram of a method for producing a food for the improvement of micro-dust stimulation respiratory disease comprising the seaweed of the invention.
Figure 2 is a graph showing the results of inhibition of fine dust-induced lipid peroxidation of seaweed and mixed plant extracts.
Figure 3 is a graph showing the effect on protection of PM-induced cell death against Raw264.7 cell line of seaweed products.

In one aspect, the present invention,

a) pretreatment of the collected seaweed;

b) freezing the pretreated seaweed to −30 to −40 ° C .;

c) thawing by immersing the frozen seaweed in wash water;

d) spraying and washing air into seaweed at the same time as the thawing;

e) screening seaweed ears by removing the washing water and foreign matter contained therein;

f) supplying the wash water to seaweed ears to perform the second washing;

g) cutting the washed seaweed into a certain size;

h) blanching the cut seaweed;

i) cooling the poached seaweed slice;

j) fermenting and aging by immersing the cooled seaweed slice in a complex plant extract;

k) packaging the seaweed and complex plant extracts which have been matured into a unit of product; And

l) sterilizing the packaged product;

The complex plant extract provides a method for producing a food for improving the fine dust irritation respiratory disease, characterized in that the extract is prepared by extracting a mixed plant, including seaweed, shiitake mushroom or militaris Cordyceps sinensis, gilyeong, windproof and cypress.

In another aspect, the present invention,

The complex plant extract is

j1) individually pretreatment of plants including seaweed, shiitake mushrooms or militaris cordyceps, gilyeong, windproof and cypress;

j2) drying the pretreated plant;

j3) mixing the dried seaweed 40 to 60% by weight, 15 to 30% of shiitake mushrooms or Militaris cordyceps, 15 to 30% by weight, windproof 5 to 12% by weight, and 3 to 10% by weight of cypress;

j4) adding a solvent of 5 to 25 times the weight of the mixed plant by weight to obtain a extract by primary extraction for 7 to 48 hours at 0.5 to 0.6 atm and a temperature of 60 to 80 ℃;

j5) filtering the obtained extract to filter foreign matter;

j6) filtering foreign matter and adding 5 to 25 times the solvent by weight to the remaining solids to obtain an extract for 2 to 3 hours at 0.8 to 0.9 atm and a temperature of 80 to 95 ° C. for extraction;

j7) filtering the obtained extract to filter foreign matter;

j8) combining the extracts obtained by the first and second extraction and concentrating the brix ° of solids to 40 or more for 4 to 8 hours under a reduced pressure of -0.08 to -0.09 MPA at a temperature of 50 to 65 ° C; It provides a method for producing a food for the improvement of fine dust stimulation respiratory disease characterized in that it is produced by.

In another further aspect of the present invention,

In the extraction process, the extraction solvent provides water, alcohol, methanol and ethanol provides a method for producing a food for improving the fine dust stimulation respiratory disease, characterized in that at least one selected from the group consisting of.

In yet another aspect, the present invention,

It provides a food for the improvement of fine dust stimulation respiratory disease characterized in that it is produced by the above-described method.

Hereinafter, with reference to the accompanying drawings, the food for improving the fine dust stimulation respiratory disease, including the seaweed ear according to the present invention and a manufacturing method thereof will be described in more detail.

Hereinafter, the main material used for this invention is demonstrated.

Seaweed ear is a year-old seaweed belonging to the balsam family of brown algae, and contains minerals and physiologically active ingredients such as calcium, potassium, iron, and iodine. Especially, the viscous substance and polysaccharide have the effect of absorbing and excreting heavy metals from food contaminated with pesticides. In addition, alginic acid, an important component of brown algae cell membrane, is a copolymer of D-mannuronic acid and L-glucuronic acid.

Shiitake mushrooms (Lentinus edodes) belongs to the genus Pleurotus eryngii, which is a parasitic fungi parasitic in hardwoods such as oak trees. It has a flavor and pharmacological effect and is widely used for food and medicinal purposes in Korea.

Shiitake mushroom extract is listed as a food supplement for health functional food standard by the Ministry of Food and Drug Safety, but there is no data on the respiratory protection effect on the above material.

Cordyceps sinensis, along with wild ginseng and deer antler, is one of the three major medicinal herbs around the world, and about 300 species are distributed around the world. Among them, Cordyceps militaris has been reported to contain biomodulators such as Cordycepin, which exhibits antimicrobial activity, and also has been shown to be effective in improving anti-obesity, anti-allergy and liver function. It is attracting attention as a material for functional foods, health supplement foods, and new drugs.

However, the number of wild fruiting bodies of Militaris cordyceps is limited, and economical artificial cultivation methods for live insects have not been developed, and therefore, they are limitedly produced using brown rice, which is a vegetable material. Militaris cordyceps, produced on living insect hosts, has been reported to contain abundant functional substances because it occurs through metabolic processes very similar to the principle of cordyceps in nature. In other words, the fruiting body corresponding to mushrooms is rich in substances that are excellent in anti-obesity, anti-allergy, liver function improvement effect, and the host pupa contains a large amount of immune enhancing substances.

Gilico (Platicodon grandiflorum A DC), also called bellflower, dorat, gilkyungchae, white medicine, plantain, and mountain bellflower, is a perennial herb that grows in mountains and has a height of 40-100 cm. In spring and autumn, roots are taken and eaten raw or as herbs.

The pathogen of the herbal medicine is peeled or dried as root, and about 2% of saponin at the root, 3-O-β-glucosylplaticodigenin, α-spinasterol, coumarin, Resin, oil, a small amount of alkaloids, a large amount of inulin, Platicodinin, etc., and the bellflower saponin has a hemolytic action, and in the civilian use is used for head pain, scarlet fever, cholera, gastritis, redness.

Windbreak (Saposhnikoviae divaricata Schiskin) is a perennial herb, belonging to the Umbelliferae family, which is a perennial herb that is called perennial wind, mountain wind, screen sprout, mountain wind tree, wind root, etc. It is widely distributed in Korea, China, Vietnam and Mongolia. The main effect of the windproof by oriental medicine is sweating, antipyretic, analgesic, diuretic, palsy, limb paralysis, Jinhae, expectorant, toothache, neuralgia, rheumatism, etc., and is known to have antibacterial and viral inhibitory effects.

Chamaecyparis obtusa is an evergreen tree of the Cypressaceae family, also known as blackwood, hinoki and cypress. Its height is 30 ~ 40m, its width is about 1 ~ 2m, the bark is reddish brown, and small needle-shaped leaves grow in the branches. It is distributed in Japan and southern Korea, and contains γ-cardinene, α-terpineol and borneol, and leaves and wood contain 1% essential oil. . It is used as a natural insecticide to fight the house dust mite, which acts to produce antibodies to the antigen to improve the body's cotton softness, causing allergic diseases such as atopy, asthma and rhinitis. It is good to use extracts of leaves, stems and branches. The wind and cypress extracts may be used alone or in the form of a mixture.

These complex plant extracts, including seaweed, shiitake mushroom or militaris Cordyceps sinensis, Giltyeong, windbreak, and cypress, are in harmony with each other to protect the liver and stomach of the patient, neutralize toxicity, and be caused by fine dust. It can provide various benefits such as being effectively used for the improvement of respiratory diseases including bronchitis.

Method for producing a food for the improvement of fine dust stimulation respiratory disease, including the seaweed ear of the present invention is largely as shown in Figure 1 seaweed ear pretreatment step, freezing step, thawing step, washing step, screening step, secondary washing step, cutting It may comprise a step, a decimation step, a cooling step, a fermentation ripening step, a sterilization step and a packaging step, wherein the plant mixture extract comprises a pretreatment step, a drying step, a mixing step, an extraction step, a filtration step, a repeating step and a concentration step Can be done. The following describes the individual steps in detail.

a) seaweed pretreatment step

In general, plant samples can cause extract loss and major component content change due to washing, so it is recommended to use a process that minimizes damage to plant tissue when washing raw materials. It is a good idea to clean and filter out foreign objects. In particular, seaweed ears need to be washed several times in running water to remove foreign substances and salts from the surface. However, this step can be omitted in situations where urgent processing is required.

b) freezing step

Pre-treated or untreated seaweeds are stored in freezer for -30 ~ -40 ℃ to store for several days to 1 year.

c) thawing stage

The frozen seaweed is removed from the freezer and thawed by soaking it in washing water containing 10-20 ℃ purified water or groundwater. In this process, the active ingredients are present in the seaweed ears, and various suspended matters attached to the ice film and the salts contained in the inside of the seaweed tissue are desalted to remove the salts of the seaweed ears.

d) washing step

Simultaneously with the thawing, the air is blown into the seaweed ear using an air blower to wash the seaweed ear to help desorption of salts and foreign substances coming out in the thawing process.

Simply washing the seaweed ear removes the salt only on the surface of the seaweed, so even if washed several times, the salt does not desorb inside the seaweed, there is a significant salinity in the commercialization. The present invention is technically characterized in that the active ingredients including alginic acid and the like present in the seaweed ears are removed to the extent necessary for the salinity inside the seaweed ears by the thawing and washing steps.

e) screening step

From the seaweed, which has been washed, removes the water containing foreign substances and salt, and selects and removes the foreign substances.

f) second washing step

Washing water is sprayed on the seaweed ear after the screening process, the seaweed ear is washed again, cleaned, and dried.

g) cutting step

The dried seaweed ears are sliced into thin slices and cut into small sizes that can be conveniently used by consumers using an automatic cutting device. It is not necessary to limit the size, but it is sufficient for the consumer to use it as a side dish or a favorite food, and it may be desirable to cut the slice into 3 to 10 cm in length and 2 to 6 mm in thickness.

h) needle stage

A step of softening the tissue while maintaining the texture by scrubbing the sliced seaweed slices, while boiling the sliced seaweed in hot water or boiling water for 10 to 40 seconds, or supplying steam at 85-95 ° C to an external tube. By passing through the inner tube of the double tube to be sequentially can be carried out for 2 to 5 minutes.

i) cooling stage

The cooling step may be performed for 2 to 5 minutes by naturally cooling the poached seaweed slice or by sequentially passing through the inner tube of the double tube continuously connected to the double tube and supplied with cooling water to the outer tube.

j) fermentation and ripening steps

Subsequently, the cooled seaweed ear is immersed in the complex plant extract for fermentation and maturation. The fermentation and aging step is further subdivided into j1) immersion step, j2) microbial inoculation step, j3 fermentation step, j4) enzyme addition step and j5) aging. It may be desirable to consist of steps.

j1) immersion step

First, boiled seaweed ears are immersed in the complex plant extract so that the seaweed ears are sufficiently submerged.

j2) microbial inoculation step,

Subsequently, the fermentation strain is inoculated at a concentration of 0.1 to 3% by weight in the complex plant extract immersed in seaweed.

The complex plant extract is prepared by extracting a mixed plant including seaweed, shiitake mushroom, militaris Cordyceps sinensis, Giltyeong, windbreak, and cypress, which will be described in detail later.

Preferred fermentation strains for inoculation at this stage may be preferably used as long as the microorganisms are beneficial for improving the functionality of seaweed and complex plant extracts and facilitate the absorption of useful components in the form of polymers.

For such a fermentation strain may preferably be used to Bacillus (Bacillus) genus Lactobacillus bacteria (Lactobacillus), A saccharide in my process (Saccharamyces) genus Pseudomonas or the genus Cellulofine (Cellulomonas).

In particular, Bacillus subtilis , Bacillus natto , Bacillus amyloliquefaciens , Lactobacillus acidophilus , Lactobacillus plantarum , Lactobacillus plantarum Lactobacillus brevis (Lactobacillus brevis), Lactobacillus casei (Lactobacillus casei), Lactobacillus buffer momentum (Lactobacillus fermentum), Lactobacillus ramno suspension (Lactobacillus rhamnosus), Lactobacillus ruteri (Lactobacillus reuteri), saccharose in my process three Levy jiae (Saccharomyces cerevisiae), saccharose in my process ellipsis Soy Ranges (Saccharomyces ellipsoideus), saccharose in my process formate Mohsen sheath (Saccharomyces formosensis), a saccharide My process car's Bergen sheath (Saccharomyces carlsbergensis), coarse My process mandrel Shurian a saccharide ( Saccharomyces mandshuricus ) and Saccharomyces corea nus ), Cellulomonas cellulans , Streptococcus thermophilus and Leuconostoc mesenteroides , Phototrophic bacteria , Yeast, EM bacteria (Useful microorganisms) may be used in combination, or commercially available strains may be used.

In addition, those deposited with microorganisms can be obtained and used, such as Bacillus subtilis (KCTC 3239), Lactobacillus acidophilus (KCTC 3111), Lactobacillus Lactobacillus plantarum (KCTC 3104), Lactobacillus acidophilus NCFM (ATCC Accession No. SD5221), Lactobacillus salivarius Ls-33 ( Lactobacillus salivarius Ls-33, ATCC Accession No. SD5208) and Lactobacillus Bacillus plantarum Lp-115 ( Lactobacillus plantarum Lp-115, ATCC Accession No. SD5209), Saccharomyces cerevisiae (KCTC 7915) strains.

j3) fermentation step,

The inoculum is then fermented at 25-43 ° C. for 3-10 days. Fermentation may be carried out with stirring or if necessary on a rotary fermenter.

j4) enzyme addition step

In addition, the fermentation step is laccase, cellulase, pectinase, xylanase, polygalacturonase, termamyl, amylase And one or more enzymes selected from the group consisting of glucoamylase and saccharides including monosaccharides, disaccharides, or polysaccharides, and thus may be efficient in reducing and degrading useful components such as carbohydrates. You may use the said commercially available enzyme.

The sugars may include monosaccharides, disaccharides, polysaccharides, and examples thereof include glucose, fructose, maltose, starch and the like. In addition, sugar, starch syrup or honey may be used. The addition amount of the enzyme is preferably used in 1 to 5% by weight based on the fermentation broth.

j5) ripening step

After fermentation is completed, fermented products are aged at room temperature in a well-ventilated shade for 3 to 10 days. Through this aging process, fermentation is sufficiently matured, and useful functional ingredients are dissolved in fermentation stock and various enzymes, coenzymes, and vitamins are used. In addition to containing a large amount of active ingredients, such as organic acids, minerals, it may be desirable to be in a state in which the functionality is increased, the flavor and taste are increased, and the digestion and absorption is good.

k) packing step

After maturation, seaweed and complex plant extracts are packaged into products as standard units. The finished product in this step is subdivided, weighed, filled and sealed to contain a certain amount.

The filling and sealing process may use an automatic packaging machine or a pouch packing machine, a band sealer, or a rotary type automatic machine.

l) sterilization step

The packaged product can be sterilized in a conventional manner to ensure shelf life and shelf life of the product. For example, the package is sterilized by retort sterilization, ultraviolet sterilization, or wet sterilization as necessary to increase the shelf life.

Subsequently, in accordance with the standards and specifications, the properties, foreign matters, moisture, bacteria count, E. coli, etc. are inspected and shipped only to suitable products.

Hereinafter, it demonstrates with respect to manufacture of the said complex plant extract. The plant mixture extract may comprise a pretreatment step, drying step, mixing step, extraction step, filtration step, repetition step and concentration step.

j1) pretreatment step

As a step of individually pretreating plants including seaweed ears, shiitake mushrooms, Militaris Cordyceps sinensis, Gilkyung, windproof and cypresses, the soil and foreign substances are removed by pretreatment according to the pretreatment of seaweed ears.

In addition, when purchasing a raw material, it is necessary to confirm that it satisfies the standards and standards set forth in the Food Code, Food Additive Code, and the like.

j2) drying step

After washing, it is preferable to dry the plant materials in a well-ventilated place, either naturally or by hot air drying at a temperature of 55-65 ° C. using low temperature drying or a dryer. At this time, the dry state is sufficient if the water is removed.

j3) mixing step

Dried plant materials are mixed at the rate of 40 ~ 60% by weight seaweed, 15-30% by shiitake mushroom or Militaris cordyceps, 15-30% by length, windproof 5-12% by weight and cypress 3-10% by weight As necessary, it is also possible to omit this step as necessary and to extract and use the plant materials individually, but in view of increasing the functionality, in the present invention, a mixed extraction was used in consideration of physical conditions such as processing time.

When the amount of seaweed ear is less than 40% by weight, the effect of improving respiratory disease is insignificant, and when it exceeds 60% by weight, there is no synergistic effect depending on the amount added. In addition, the shiitake mushroom or Militaris Cordyceps sinensis may have a weak synergistic effect at 15 wt% or less, and if it exceeds 30 wt%, the synergistic effect of the combination with each component may be inferior. In addition, the gilyeong extract, windproof root extract or cypress extract is less than a predetermined weight percent respiratory disease improvement effect, if exceeded, it is not economical because it is not balanced with the overall composition, stable harmony of the overall composition within the above set range And various functions can be exhibited optimally.

j4) complex plant extraction step

5 to 25 times the number of solvents are added to the mixed plants in a certain ratio, and the extract is obtained by first extracting at a temperature of 0.5 to 0.6 atm and a temperature of 60 to 80 ° C. for 7 to 48 hours.

Experimental results of setting the temperature conditions for standardizing the temperature conditions for extracting the mixed plants showed that the extraction of the mixed plants was most effective for 7 to 48 hours at 0.5 to 0.6 atm, 60 ℃ -70 ℃.

Meanwhile, as the extraction solvent, one or more selected from the group consisting of water, alcohol, methanol, and ethanol may be used, and the amount of the solvent shows good results when extracted with 5 to 25 times the results of experiments for standardizing solvent usage. In comparison with economics, the solvent may be most effective to extract by adding 10 times.

Therefore, for mass production, the mixed plant extraction solvent may be 10 times, the extraction temperature is 65 ℃, the extraction time may be the most desirable 24 hours.

j5) filtration step

After the extraction step is completed, the solids are first filtered using a filtration membrane, paper filter paper, or nonwoven fabric. Since the nonwoven fabric is filtered to fine dust, clay, and foreign matter, it is preferable to filter with a nonwoven fabric without using other cloths or objects. .

j6) and j7) repeat steps

Filter out foreign matter and add 5 ~ 25 times of solvent by weight to the remaining solids, extract for 2 to 3 hours at 0.8 ~ 0.9 atm and 80 ~ 95 ℃ to obtain extract and filter it to filter out foreign matter. . At this stage, useful components which are not extracted by the primary extraction conditions may be further extracted and used.

j8) concentration step

After combining the extracts obtained by the first and second extraction, the brix ° of the solid content is concentrated to 40 or more for 4 to 8 hours under a reduced pressure of -0.08 to -0.09 MPa at a temperature of 50 to 65 ℃. The yield was the best when concentrated under reduced pressure of -0.08 ~ -0.09 MPa using a vacuum concentrator and the color was dark brown liquid. In this step, a rotary vacuum evaporator may be used.

The food for improving the fine dust stimulation respiratory disease according to the present invention inhibited the lipid peroxidation reaction caused by the fine dust in the MDA production inhibition rate test, as confirmed in the test example described below, and further, by virtue of the fine dust treatment through the MTT assay, cell viability As a result, the synergistic effect on the inhibitory effect on apoptosis was confirmed in the group treated with Wakame complex plant extract.

Therefore, the food containing seaweed ears according to the present invention is considered to be possible to use as an active ingredient of a medicinal or health functional food for preventing or improving respiratory diseases caused by fine dust.

In the present invention, the fine dust-induced respiratory diseases include respiratory inflammatory lung disease, chronic obstructive pulmonary disease, sinusitis, allergic rhinitis, lower respiratory tract infection, acute bronchitis, emphysema, pneumonia, asthma, bronchitis, bronchodilation, pharyngitis, tonsillitis, and laryngitis It may include one or more diseases selected from the group consisting of.

The medicine of the present invention can be administered by various routes. Various modes of administration can be envisaged, for example, by oral, rectal or intravenous, intramuscular, or subcutaneous injection.

In addition, the pharmaceutical composition may be administered in a variety of oral and parenteral formulations during actual clinical administration, and when formulated, diluents or excipients such as fillers, weights, binders, wetting agents, disintegrating agents, surfactants, etc. that are commonly used. Can be used.

Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, which include, for example, starch, calcium carbonate, sucrose or lactose, It is prepared by mixing gelatin. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. .

Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and the suspension solvent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used.

Dose of the seaweed-containing food composition of the present invention may vary depending on the patient's worm, age, sex, health status, diet, administration time, administration method, excretion rate and the severity of the disease, depending on the judgment of the doctor or pharmacist It may be administered once or several times a day at intervals of time. For example, the daily dose may be 0.001 to 500 mg / kg, preferably 0.1 to 200 mg / kg, based on the active ingredient content.

In addition, the functional food composition according to the present invention may be provided in a formulation including, for example, powder, granules, pills, tablets, capsules, pouches, liquid or beverage forms according to conventional methods in the art.

The intake amount of the food of the present invention is appropriately set according to the form of the preparation, the method of administration, the purpose of use, and the age, weight, and symptoms of the individual to which it is applied, and the amount of the active ingredient contained in the preparation is generally not constant. Per day, such as from 0.001 mg / kg to 500 mg / kg. Of course, since the dose varies depending on various conditions, an amount smaller than the intake amount may be sufficient, or it may be necessary beyond the range.

In addition, when the composition of the present invention is prepared as a functional food (health functional food and general food) composition may include ingredients that are commonly added to food, for example, proteins, carbohydrates, fats, sweeteners, such as For example, licorice, vitamin C, citric acid, nicotinic acid, sodium benzoate, aspartame, saccharin, pectin, malitol, sorbitol, xylitol, guar gum, skim milk powder and oligosaccharides can be added to enhance palatability or taste. You can.

They are suitably used at about 0.01 to 20% by weight based on the total weight of the composition of the present invention.

Drinking water is, for example, put the seaweed ear containing fermented matured at 0.01 to 20% by weight, licorice 0.01-2% by weight, citric acid 0.01-2% by weight, malic acid 0.01-2% by weight, taurine 0.1-2% by weight , 0.01 to 2% by weight of vitamin C, 0.01 to 2% by weight of vitamin B1, 0.01 to 2% by weight of biotin, 0.01 to 20% by weight of liquid fructose, 0.1 to 3% by weight of polydextrose, 0.1 to 1% by weight of pear concentrate, 0.1 to 0.5 of honey Weight%, calcium lactate 0.01 to 1% by weight, fragrance 0.1 to 1% by weight, pigment 0.01 to 0.05% by weight, sodium citrate 0.01 to 0.5% by weight, stebitene fresh 0.01 to 0.5% by weight, nicotinic acid amide 0.01 to 0.5% by weight, Royal jelly extract 0.01 ~ 0.5% by weight, L- menthol 0.0001 ~ 0.5% by weight or the like can be added to prepare a functional beverage.

<Example>

Hereinafter, the present invention is described in more detail by the following representative examples, but the present invention is not limited in any way by these examples. Each value is the mean ± standard deviation of three replicates.

Example  1: Preparation of Complex Plant Extracts

Plants including seaweed, shiitake mushrooms, militaris cordyceps, gilyeong, windproof and cypress are individually washed to remove foreign substances, and left in a well-ventilated place for 12 hours to dry naturally and mixed in the mixing ratio shown in Table 1 below. 100 Kg of a composite plant was put in a 1 Ton extractor, and 5 times of alcohol was added thereto, followed by extraction at 0.5 atmosphere and a temperature of 65 ° C. for 10 hours to obtain a primary extract. After filtering it with a nonwoven fabric, 600 liters of purified water was added to the remaining solid, and then extracted for 2 hours at 0.8 atm and 85 ° C. for 2 hours to filter the solids. The extract obtained by the first and second extraction was 1: 1. Combined by weight ratio and concentrated for 4 hours under reduced pressure of -0.08 MPa at a temperature of 55 ° C. Solid brix was measured between 53 and 56.

Ingredient Content (kg) Example 1 Example 2 Example 3 Example 4 Example 5 Comparative example Seaweed Ear 40 45 50 40 60 35 Shiitake mushrooms - 30 - 15 - - Militaris Cordyceps Sinensis 30 - 15 - 15 - Street view 15 17 20 30 15 20 Windproof 10 5 5 12 5 25 White 5 3 10 3 5 20 Sum 100 100 100 100 100 100

Example  6: Preparation of food containing seaweed ears

After washing 100Kg of the collected seaweeds in running water, freeze it at -35 ℃ and store it for 3 months, then desorb it by spraying an air blower while it is immersed in 13 ~ 15 ℃ washing water. After discarding the washing water and sprayed again with fresh new spinal cord, and then dried to some extent cut to a length of 3cm, it was passed through the inside of the double pipe flowing steam to the outside and then cooled through the same double pipe. Then, after immersing in 250Kg of the composite plant extract prepared in Example 3, the fermentation strain was inoculated with 1Kg and then fermented at 30 ° C for 3 days. In the fermentation step, 5 Kg of cellulase and sugar were added. Fermentation is completed fermentation was aged for 3 days at room temperature in a well-ventilated shade, sealed packaging in small units and then retort sterilized to prepare a product. In the following test example, the aged fermented product was ground.

Test Example

In the following test examples, in vitro efficacy in respiratory tissues and tests for suppressing the inflammatory response of bronchial epithelial cells and macrophages were performed.

In all experiments, the efficacy of the product of Example 6 and that of the comparative example was evaluated. Fine dust was added at a concentration of 50 µg / mL which does not significantly affect cell survival. Each value represents the mean ± SD of three measurements.

Test Example  1: Preparation of experimental animal lung tissue

Experimental animals (ICR mouse, male, 8 weeks) were fasted for 24 hours before dissection, and then opened under ether anesthesia, and lung tissue was separated and stored at -70 ° C until analysis. Lung tissue was washed several times with sterile physiological saline, lysis buffer was added, lysis and crushing were centrifuged (10,000 x g), and lung tissue homogenates were prepared. Proteins were quantified by BCA kit, and the samples were titrated (100 ㎍-1,000 ㎍), and the samples were treated with lung tissue homogenate for 1 hour or fine dust + sample, followed by in vitro testing.

Test Example  2: Lipid Peroxidation Analysis of Food Ingredients Containing Seaweed in Lung Tissue

In order to analyze the antioxidant activity of the seaweed ear containing food according to Example 6 after the fine dust stimulation was evaluated the lipid peroxidation inhibitory ability in the lung tissue. Lipid peroxidation was analyzed through MDA production, and MDA production in tissue homogenates of samples and controls was measured and compared.

The vehicle was used instead of the sample as a general control group, and the negative control group was used to give only fine dust stimulation (50㎍ / mL) to the lung tissue homogenate and to stimulate only FeSO ₄ , and the positive control group was ascorbic acid (10). ㎍) + fine dust stimulation (50 μg / mL), gallic acid (10 μg) as polyphenol + fine dust stimulation (50 μg / mL), and naringin (10 μg) as flavonoid + fine dust stimulation (50 μg / mL) Was performed. The experimental group was carried out at the concentration of 5 μg, 10 μg, 20 μg + fine dust stimulation (50 μg / mL) in the seaweed-containing food (powder-containing liquid) of Example 6. Figure 2 is a graph showing the results of inhibiting fine dust-induced lipid peroxidation of seaweeds containing foods.

As a result, the amount of MDA produced by the normal control treated with the buffer in which the sample was dissolved was 3.6273 ± 0.1076 nmol MDA / g, and the amount of MDA produced by the microcontrolled negative control was 4.5625 ±± 0.1267 nmol MDA / g and negatively stimulated with FeSO ₄ . MDA production in the control group was 5.1724 ± 0.1356 nmol MDA / g, in the positive control ascorbic acid + fine dust 4.0632 ± 0.2172 nmol MDA / g, in the gallic acid + fine dust 4.4205 ± 0.1363 nmol MDA / g, and in the positive control naringin + fine dust 4.4302 ± 0.2727 nmol MDA / g, food containing seaweed ear, experimental group 4.3266 ± 0.2691 nmol MDA / g, 10μg + 3.9653 ± 0.2126 nmol MDA / g, 20μg + fine dust The production amount of nmol MDA / g was shown.

These results indicate that the amount of MDA produced during stimulation of fine dust in lung tissue was lower than the amount of MDA produced during FeSO ₄ stimulation. Indicates that MDA production was inhibited.

Experimental Example  3: bronchial inflammation inhibition test

In order to confirm whether the food prepared in Example 6 has an inhibitory effect on bronchoinflammatory inflammation in the asthma and chronic obstructive pulmonary disease model, the leukocyte increase response of bronchiole caused by exposing the antigen to sensitized mice was confirmed (Pharmacological Research, 2010, 61, 288-297; Biochemical Pharmacology, 2010, 79, 888-896).

To this end, 10 μl of egg albumin (Ovalbumin, OVA, sigma) and aluminum hydroxide (4 mg, aluminum hydroxide, Alum, Pierce) 1: 1 mixed solution in experimental animals intraperitoneally on days 0, 7, and 14 Sensitized by administration. After 8 days and 10 days after the final sensitization, 10% egg albumin was aerosolized using high-pressure compressed air and sprayed for 50 minutes to induce airway inflammation. Administration of each test substance (00 mg / kg, bid) was challenged by oral administration twice a day in the afternoon from 21 to 23 days after the first sensitization. Bronchoalveolar lavage was collected by bronchoalveolar lavage using 15 ml of phosphate buffer (pH 7.2) 24 hours after the last challenge. White blood cell counts in the washes were counted using a hematology analyzer (Hematology analyzer, Drew Scientific Inc, HEMAVET HV950FS, M-950HV). The results are shown in Table 2 by calculating the inhibition rate of bronchial inflammation based on the leukocyte cell count of the negative control group (1% CMC [carboxymethyl cellulose] administration group).

Inflammation inhibition rate (%) Example 6 32.8 ± 1.06 Comparative example 5.52 ± 0.27

As shown in Table 2, the food according to the embodiment of the present invention was found to have an excellent effect of inhibiting the white blood cell count of the bronchoalveolar lavage fluid, it was confirmed that the extract is excellent in the inhibition of inflammation.

Test Example  4: Determination of Cell Viability by Fine Dust of Seaweeds MTT  assay

MTT assay was performed using Raw264.7 cell line (Korea Cell Line Bank, 40071) in order to investigate the inhibitory effect of the reduction of cell viability of seaweed ear containing products by the toxicity of fine dust.

Raw264.7 cells were added with PM10 (NIST, 1648a, 50μg / ml) when cells were cultured at 90-100% after 24 hours in RPMI-1640 media containing 10% fetal bovine serum (FBS). Was performed only in cell culture and not treated with PM10.

After dispensing cells into 96-well plates and incubating for 12 hours, each sample was treated with cells and incubated for 24 hours, after which MTT solution (final concentration: 0.5 mg / mL) was added and further 4 hours at 37 ° C. The culture was removed to prevent formazan from culturing by reducing MTT, and 100 μl of DMSO was mixed for 10 minutes, and then absorbance was measured at 540 nm.

The results are shown in FIG. Figure 3 is a graph showing the effect on protection of PM-induced cell death against Raw264.7 cell line of seaweed products.

As a result, the survival rate was reduced to about 43% in the microdust-treated group, while the samples containing seaweeds were 55, 66, 76, 82, and 89 in the 10, 20, 30, 50, and 100 μg / ml concentrations, respectively. Cell viability was shown as%, cell viability increased with increasing concentration. From these results, it was confirmed that the microdust-induced cell protective effect of seaweeds containing products.

Test Example  5: sensory evaluation

As for the sensory test on the seaweed return product prepared according to Example 6, 30 sensory test personnel with sensory discrimination ability were selected, and the evaluation scale of the quality was 7-point scale (7 points: very good, 6 points: good. : A little good, 4 points: normal, 3 points: a little bad, 2 points: bad, 1 point: very bad).

The sensory test items were evaluated by five sensory items such as texture, texture, flavor, and overall acceptability. The results were verified by Duncan's multiple range test at 5% level using ANOVA. After scoring the result, it was averaged and shown in Table 3 (mean value + standard deviation).

Item Example 6 Comparative example Texture 6.85 ± 1.26 5.51 ± 1.22 Umami 6.72 ± 0.35 5.23 ± 1.53 incense 6.43 ± 1.05 5.62 ± 1.10 Overall preference 6.65 ± 0.87 5.35 ± 0.82

As a result, the product of Example 6 was very excellent in texture and texture, aroma and overall acceptability, and showed a significantly better result than the comparative example in all items. As a result of Table 3, it was confirmed that the products of the embodiments of the present invention are comprehensively superior to the comparative product regardless of age group.

As confirmed in the above examples and test examples, the seaweed ear containing food according to the present invention is determined that the extract can be used as a health functional product for preventing or improving respiratory diseases caused by fine dust.

As mentioned above, although an exemplary embodiment of the present invention has been described with reference to the accompanying drawings, those skilled in the art to which the present invention pertains may change to other specific forms without changing the technical spirit or essential features of the present invention. It will be appreciated that it may be practiced. Therefore, it should be understood that one embodiment described above is illustrative in all respects and not restrictive.

Claims (4)

a) pretreatment of the collected seaweed;
b) freezing the pretreated seaweed to −30 to −40 ° C .;
c) thawing by immersing the frozen seaweed in wash water;
d) spraying and washing air into seaweed at the same time as the thawing;
e) screening seaweed ears by removing the washing water and foreign matter contained therein;
f) supplying the wash water to seaweed ears to perform the second washing;
g) cutting the washed seaweed into a certain size;
h) blanching the cut seaweed;
i) cooling the poached seaweed slice;
j) fermenting and aging by immersing the cooled seaweed slice in a complex plant extract;
k) packaging the seaweed and complex plant extracts which have been matured into a unit of product; And
l) sterilizing the packaged product;
The complex plant extract is prepared by extracting a mixed plant comprising seaweed, shiitake mushroom or militaris Cordyceps sinensis, Giltyeong, windbreak and cypresses. The method according to claim 1,
The complex plant extract is
j1) individually pretreatment of plants including seaweed, shiitake mushrooms or militaris cordyceps, gilyeong, windproof and cypress;
j2) drying the pretreated plant;
j3) mixing at a rate of 40 ~ 60% by weight seaweed, 15-30% of shiitake mushrooms or Militaris Cordyceps sinensis, 15-30% by weight, windproof 5-12% by weight and cypress 3-10% by weight;
j4) adding a solvent of 5 to 25 times the weight of the mixed plant by weight to obtain a extract by primary extraction for 7 to 48 hours at 0.5 to 0.6 atm and a temperature of 60 to 80 ℃;
j5) filtering the obtained extract to filter foreign matter;
j6) filtering foreign matter and adding 5 to 25 times the solvent by weight to the remaining solids to obtain an extract for 2 to 3 hours at 0.8 to 0.9 atm and a temperature of 80 to 95 ° C. for extraction;
j7) filtering the obtained extract to filter foreign matter;
j8) combining the extracts obtained by the first and second extraction and concentrating the brix ° of solids to 40 or more for 4 to 8 hours under a reduced pressure of -0.08 to -0.09 MPA at a temperature of 50 to 65 ° C; Method for producing a food for the improvement of fine dust stimulation respiratory disease characterized in that it is produced by. The method according to claim 2,
Extraction solvent in the extraction process is water, alcohol, methanol and ethanol is a method for producing a food for improving the fine dust stimulation respiratory disease, characterized in that at least one selected from the group consisting of. Food for improvement of fine dust stimulation respiratory disease, characterized in that produced by the method according to claim 1.

Images